BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,032,284	T→C	D304G (GAT→GGT)	lysS ←	lysine tRNA synthetase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,032,284	0	T	C	99.4%	1042.2 / ‑3.5	331	D304G (GAT→GGT)	lysS	lysine tRNA synthetase
Reads supporting (aligned to +/- strand): ref base T (1/1); new base C (115/214); total (116/215)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.33e-01

Only 100 of 350 total aligned reads displayed.

TTGATCGCTTCACGCATGGTCAGTTTTTCGAACGGTTTACCGAAGTCCAGCGTCACGTCGCCGTAGGTCACTTCCGTCTTACCGAGAATATCCTGTGCCAGAGTACGGAACAGCGATTCGGTCAGCTCGATCAGATCTTTGTAATCTGCGTAAGCCATGTAGAGTTCCATCATGGTGAAC  >  NC_000913/3032194‑3032373

ttGATCGCTTCACGCATGGTCAGTTTTTCGAACGGTTTACCGAAGTCCAGCGTCACGTCGCCGTAGGTCACTTCCGTCTTACCGAGAATAccc                                                                                         >  2:7625962/1‑93 (MQ=255)

       cTTCACGCATGGTCAGTTTTTCGAACGGTTTACCGAAGTCCAGCGTCACGTCGCCGTAGGTCACTTCCGTCTTACCGAGAATACCCTGTGCCa                                                                                  >  1:1314830/1‑93 (MQ=255)

        ttCACGCATGGTCAGTTTTTCGAACGGTTTACCGAAGTCCAGCGTCACGTCGCCGTAGGTCGCTTCCGTCTTACCGAGAATACCCTGTGCCag                                                                                 >  2:3834895/1‑93 (MQ=255)

         tCACGCATGGTCAGTTTTTCGAACGGTTTACCGAAGTCCAGCGTCACGTCGCCGTAGGTCACTTCCGTCTTACCGAGAATACCCTGTGCCaga                                                                                <  1:7387209/93‑1 (MQ=255)

         tCACGCATGGTCAGTTTTTCGAACGGTTTACCGAAGTCCAGCGTCACGTCGCCGTAGGTCACTTCCGTCTTACCGAGAATACCCTGTGCCaga                                                                                <  2:164392/93‑1 (MQ=255)

          cACGCATGGTCAGTTTTTCGAACGGTTTACCGAAGTCCAGCGTCACGTCGCCGTAGGTCACTTCCGTCTTACCGAGAATACCCTGTGCCagag                                                                               <  1:1427783/93‑1 (MQ=255)

          cACGCATGGTCAGTTTTTCGAACGGTTTACCGAAGTCCAGCGTCACGTCGCCGTAGGTCACTTCCGTCTTACCGAGAATACCCTGTGCCagag                                                                               <  1:1768816/93‑1 (MQ=255)

          cACGCATGGTCAGTTTTTCGAACGGTTTACCGAAGTCCAGCGTCACGTCGCCGTAGGTCACTTCCGTCTTACCGAGAATACCCTGTGCCagag                                                                               <  1:4721052/93‑1 (MQ=255)

           aCGCATGGTCAGTTTTTCGAACGGTTTACCGAAGTCCAGCGTCACGTCGCCGTAGGTCACTTCCGTCTTACCGAGAATACCCTGTGCCAGAGt                                                                              <  2:5804184/93‑1 (MQ=255)

            cGCATGGTCAGTTTTTCGAACGGTTTACCGAAGTCCAGCGTCACGTCGCCGTAGGTCACTTCCGTCTTACCGAGAATACCCTGTGCCAGAGTa                                                                             <  2:3151550/93‑1 (MQ=255)

             gCATGGTCAGTTTTTCGAACGGTTTACCGAAGTCCAGCGTCACGTCGCCGTAGGTCACTTCCGTCTTACCGAGAATACCCTGTGCCAGAGTAc                                                                            <  1:1229260/93‑1 (MQ=255)

             gCATGGTCAGTTTTTCGAACGGTTTACCGAAGTCCAGCGTCACGTCGCCGTAGGTCACTTCCGTCTTACCGAGAATACCCTGTGCCAGAGTAc                                                                            <  1:4170696/93‑1 (MQ=255)

             gCATGGTCAGTTTTTCGAACGGTTTACCGAAGTCCAGCGTCACGTCGCCGTAGGTCACTTCCGTCTTACCGAGAATACCCTGTGCCAGAGTAc                                                                            >  2:6366794/1‑93 (MQ=255)

                  gTCAGTTTTTCGAACGGTTTACCGAAGTCCAGCGTCACGTCGCCGTAGGTCACTTCCGTCTTACCGAGAATTCCCTGTGCCAGAGTACGGAAc                                                                       <  2:683108/93‑1 (MQ=255)

                  gTCAGTTTTTCGAACGGTTTACCGAAGTCCAGCGTCACGTCGCCGTAGGTCACTTCCGTCTTACCGAGAATACCCTGTGCCAGAGTACGGAAc                                                                       <  2:4934715/93‑1 (MQ=255)

                  gTCAGTTTTTCGAACGGTTTACCGAAGTCCAGCGTCACGTCGCCGTAGGTCACTTCCGTCTTACCGAGAATACCCTGTGCCAGAGTACGGAAc                                                                       >  2:2782455/1‑93 (MQ=255)

                   tCAGTTTTTCGAACGGTTTACCGAAGTCCAGCGTCACGTCGCCGTAGGTCACTTCCGTCTTACCGAGAATACCCTGTGCCAGAGTACGGAACa                                                                      <  2:7233615/93‑1 (MQ=255)

                    cAGTTTTTCGAACGGTTTACCGAAGTCCAGCGTCACGTCGCCGTAGGTCACTTCCGTCTTACCGAGAATACCCTGTGCCAGAGTACGGAACAg                                                                     <  2:4648841/93‑1 (MQ=255)

                    cAGTTTTTCGAACGGTTTACCGAAGTCCAGCGTCACGTCGCCGTAGGTCACTTCCGTCTTACCGAGAATACCCTGTGCCAGAGTACGGAACAg                                                                     >  2:7470927/1‑93 (MQ=255)

                     aGTTTTTCGAATGGTTTACCGAAGTCCAGCGTCACGTCGCCGTAGGTCACTTCCGTCTTACCGAGAATACCCTGTGCCAGAGTACGGAACAGc                                                                    >  2:7801782/1‑93 (MQ=255)

                     aGTTTTTCGAACGGTTTACCGAAGTCCAGCGTCACGTCGCCGTAGGTCACTTCCGTCTTACCGAGAATACCCTGTGCCAGAGTACGGAACAGc                                                                    <  2:1666891/93‑1 (MQ=255)

                      gTTTTTCGAACGGTTTACCGAAGTCCAGCGTCACGTCGCCGTAGGTCACTTCCGTCTTACCGAGAATACCCTGTGCCAGAGTACGGAACAgtg                                                                   >  2:366442/1‑91 (MQ=255)

                      gTTTTTCGAACGGTTTACCGAAGTCCAGCGTCACGTCGCCGTAGGTCACTTCCGTCTTACCGAGAATACCCTGTGCCAGAGTACGGAACAGCg                                                                   >  2:6446868/1‑93 (MQ=255)

                       tttttCGAACGGTTTACCGAAGTCCAGCGTCACGTCGCCGTAGGTCACTTCCGTCTTACCGAGAATACCCTGTGCCAGAGTACGGAACAGCGa                                                                  <  1:678659/93‑1 (MQ=255)

                        ttttCGAACGGTTTACCGAAGTCCAGCGTCACGTCGCCGTAGGTCACTTCCGTCTTACCGAGAATACCCTGTGCCAGAGTACGGAACAGCGAt                                                                 <  2:2231390/93‑1 (MQ=255)

                         tttCGAACGGTTTACCGAAGTCCAGCGTCACGTCGCCGTAGGTCACTTCCGTCTTACCGAGAATACCCTGTGCCAGAGTACGGAACAGCGAtt                                                                >  2:1566594/1‑93 (MQ=255)

                           tCGAACGGTTTACCGAAGTCCAGCGTCCCGTCGCCGTAGGTCACTTCCGTCTTACCGAGAATACCCTGTGCCAGAGTACGGAACAGCGATTCg                                                              <  2:2942059/93‑1 (MQ=255)

                            cGAACGGTTTACCGAAGTCCAGCGTCACGTCGCCGTAGGTCACTTCCGTCTTACCGAGAATACCCTGTGCCAGAGTACGGAACAGCGATTCgg                                                             <  2:600092/93‑1 (MQ=255)

                             gAACGGTTTACCGAAGTCCAGCGTCGCGTCGCCGTAGGTCACTTCCGTCTTACCGAGAATACCCTGTGCCAGAGTACGGAACAGCGATTCGGt                                                            <  1:6688787/93‑1 (MQ=255)

                              aaCGGTTTACCGAAGTCCAGCGTCACGTCGCCGTAGGTCACTTCCGTCTTACCGAGAATATCCTGTGCCAGAGTACGGAACAGCGATTCGGTc                                                           <  2:7274086/93‑1 (MQ=255)

                              aaCGGTTTACCGAAGTCCAGCGTCACGTCGCCGTAGGTCACTTCCGTCTTACCGAGAATACCCTGTGCCAGAGTACGGAACAGCGATTCGGTc                                                           <  2:81135/93‑1 (MQ=255)

                              aaCGGTTTACCGAAGTCCAGCGTCACGTCGCCGTAGGTCACTTCCGTCTTACCGAGAATACCCTGTGCCAGAGTACGGAACAGCGATTCGGTc                                                           >  1:2363766/1‑93 (MQ=255)

                              aaCGGTTTACCGAAGTCCAGCGTCACGTCGCCGTAGGTCACTTCCGTCTTACCGAGAATACCCTGTGCCAGAGTACGGAACAGCGATTCGGTc                                                           >  1:5158536/1‑93 (MQ=255)

                               aCGGTTTACCGAAGTCCAGCGTCACGTCGCCGTAGGTCACTTCCGTCTTACCGAGAATACCCTGTGCCAGAGTACGGAACAGCGATTCGGTCa                                                          >  2:2586999/1‑93 (MQ=255)

                                  gTTTACCGAATTCCATCGTCACGCCGCCGTAGGTCACTTCCGTCTTACCGAGAATACCCTGTGCCAGAGTACGGAACAGCGATTCGGTCAGCt                                                       <  1:2003208/93‑1 (MQ=255)

                                  gTTTACCGAAGTCCAGCGTCACGTCGCCGTAGGTCACTTCCGTCTTACCGAGAATACCCTGTGCCAGAGTACGGAACAGCGATTCGGTCAGCt                                                       >  1:3987029/1‑93 (MQ=255)

                                  gTTTACCGAAGTCCAGCGTCACGTCGCCGTAGGTCACTTCCGTCTTACCGAGAATACCCTGTGCCAGAGTACGGAACAGCGATTCGGTCAGCt                                                       >  2:6219425/1‑93 (MQ=255)

                                   tttACCGAAGTCCAGCGTCACGTCGCCGTAGGTCACTTCCGTCTTACCGAGAATCCCCTGTGCCAGAGTACGGAACAGCGATTCGGTCAGCTc                                                      <  2:5130990/93‑1 (MQ=255)

                                   tttACCGAAGTCCAGCGTCACGTCGCCGTAGGTCACTTCCGTCTTACCGAGAATACCCTGTGCCAGAGTACGGAACAGCGATTCGGTCAGCTc                                                      <  1:2105600/93‑1 (MQ=255)

                                    ttACCGAAGTCCAGCGTCACGTCGCCGTAGGTCACTTCCGTCTTACCGAGAATACCCTGTGCCAGAGTACGGAACAACGATTCGGTCAGCTCg                                                     >  2:4410465/1‑93 (MQ=255)

                                       ccGAAGTCCAGCGTCACGTCGCCGTAGGTCACTTCCGTCTTACCGAGAATACCCTGTGCCAGAGTACGGAACAGCGATTCGGTCAGCTCGATc                                                  <  2:5207191/93‑1 (MQ=255)

                                        cGAAGTCCAGCGTCACGTCGCCGTAGGTCACTTCCGTCTTACCGAGAATACCCTGTGCCAGAGTACGGAACAGCGATTCGGTCAGCTCGATCa                                                 <  1:58466/93‑1 (MQ=255)

                                         gAAGTCCAGCGTCACGTCGCCGTAGGTCACTTCCGTCTTACCGAGAATACCCTGTGCCAGAGTACGGAACAGCGATTCGGTCAGCTCGATCAg                                                <  1:7110891/93‑1 (MQ=255)

                                          aaGTCCAGCGTCACGTCGCCGTAGGTCACTTCCGTCTTACCGAGAATACCCTGTGCCAGAGTACGGAACAGCGATTCGGTCAGCTCGATCAGa                                               >  1:5644077/1‑93 (MQ=255)

                                          aaGTCCAGCGTCACGTCGCCGTAGGTCACTTCCGTCTTACCGAGAATACCCTGTGCCAGAGTACGGAACAGCGATTCGGTCAGCTCGATCAGa                                               <  1:814040/93‑1 (MQ=255)

                                            gTCCAGCGTCACGTCGCCGTAGGTCACTTCCGTCTTACCGAGAATACCCTGTGCCAGAGTACGGAACAGCGATTCGGTCAGCTCGATCAGATc                                             <  1:3356476/93‑1 (MQ=255)

                                            gTCCAGCGTCACGTCGCCGTAGGTCACTTCCGTCTTACCGAGAATACCCTGTGCCAGAGTACGGAACAGCGATTCGGTCAGCTCGATCAGATc                                             <  1:350258/93‑1 (MQ=255)

                                             tCCAGCGTCTCGTCGCCTTAGGTCACTTCCGTCTTACCGAGAATGCCCTGTGCCAGAGTACGGAACAGCGATTCGGTCAGCTCGATCAGATCt                                            <  1:944145/93‑1 (MQ=255)

                                              ccAGCGTCACGTCGCCGTAGGTCACTTCCGTCTTACCGAGAATACCCTGTGCCAGAGTACGGAACAGCGATTCGGTCAGCTCGATCAGATCtt                                           <  1:5530416/93‑1 (MQ=255)

                                              ccAGCGTCACGTCGCCGTAGGTCACTTCCGTCTTACCGAGAATACCCTGTGCCAGAGTACGGAACAGCGATTCGGTCAGCTCGATCAGATCtt                                           >  1:1696185/1‑93 (MQ=255)

                                              ccAGCGTCACGTCGCCGTAGGTCACTTCCGTCTTACCGAGAATACCCTGTGCCAGAGTACGGAACAGCGATTCGGTCAGCTCGATCAGATCtt                                           <  1:2586882/93‑1 (MQ=255)

                                               cAGCGTCACGTCGCCGTAGGTCACTTCCGTCTTACCGAGAATACCCTGTGCCAGAGTACGGAACAGCGATTCGGTCAGCTCGATCAGATCttt                                          <  1:7508226/93‑1 (MQ=255)

                                                   gTCACGTCGCCGTAGGTCACTTCCGTCTTACCGAGAATACCCTGTGCCAGAGTACGGAACAGCGATTCGGTCAGCTCGATCAGATCTTTGTaa                                      >  2:7423527/1‑93 (MQ=255)

                                                    tCACGTCGCCGTAGGTCACTTCCGTCTTACCGAGAATACCCTGTGCCAGAGTACGGAACAGCGATTCGGTCAGCTCGATCAGATCTTTGTAAt                                     <  1:6159118/93‑1 (MQ=255)

                                                    tCACGTCGCCGTAGGTCACTTCCGTCTTACCGAGAATACCCTGTGCCAGAGTACGGAACAGCGATTCGGTCAGCTCGATCAGATCTTTGTAAt                                     <  2:3085072/93‑1 (MQ=255)

                                                     cACGTCGCCGTAGGTCACTTCCGTCTTACCGAGAATACCCTGTGCCAGAGTACGGAACAGCGATTCGGTCAGCTCGATCAGATCTTTGTAATc                                    <  1:5277510/93‑1 (MQ=255)

                                                      aCGTCGCCGTAGGTCACTTCCGTCTTACCGAGAATACCCTGTGCCAGAGTACGGAACAGCGATTCGGTCAGCTCGATCAGATCTTTGTAATCt                                   <  2:1434364/93‑1 (MQ=255)

                                                      aCGTCGCCGTAGGTCACTTCCGTCTTACCGAGAATACCCTGTGCCAGAGTACGGAACAGCGATTCGGTCAGCTCGATCAGATCTTTGTAATCt                                   >  1:3875824/1‑93 (MQ=255)

                                                       cGTCGCCGTAGGTCACTTCCGTCTTACCGAGAATACCCTGTGCCAGAGTACGGAACAGCGATTCGGTCAGCTCGATCAGATCTTTGTAATCTg                                  <  2:2027341/93‑1 (MQ=255)

                                                       cGTCGCCGTAGGTCACTTCCGTCTTACCGAGAATACCCTGTGCCAGAGTACGGAACAGCGATTCGGTCAGCTCGATCAGATCTTTGTAATCTg                                  <  1:366244/93‑1 (MQ=255)

                                                       cGTCGCCGTAGGTCACTTCCGTCTTACCGAGAATACCCTGTGCCAGAGTACGGAACAGCGATTCGGTCAGCTCGATCAGATCTTTGTAATCTg                                  >  2:4180655/1‑93 (MQ=255)

                                                       cGTCGCCGTAGGTCACTTCCGTCTTACCGAGAATACCCTGTGCCAGAGTACGGAACAGCGATTCGGTCAGCTCGATCAGATCTTTGTAATCTg                                  >  2:4250368/1‑93 (MQ=255)

                                                        gTCGCCGTAGGTCACTTCCGTCTTACCGAGAATACCCTGTGCCAGAGTACGGAACAGCGATTCGGTCAGCTCGATCAGATCTTTGTAATCTGc                                 <  1:4504112/93‑1 (MQ=255)

                                                        gTCGCCGTAGGTCACTTCCGTCTTACCGAGAATACCCTGTGCCAGAGTACGGAACAGCGATTCGGTCAGCTCGATCAGATCTTTGTAATCTGc                                 <  1:2682385/93‑1 (MQ=255)

                                                           gCCGTAGGTCACTTCCGTCTTACCGAGAATACCCTGTGCCAGAGTACGGAACAGCGATTCGGTCAGCTCGATCAGATCTTTGTAATCTGCGTa                              >  1:3290154/1‑93 (MQ=255)

                                                           gCCGTAGGTCACTTCCGTCTTACCGAGAATACCCTGTGCCAGAGTACGGAACAGCGATTCGGTCAGCTCGATCAGATCTTTGTAATCTGCGTa                              >  2:4729552/1‑93 (MQ=255)

                                                           gCCGTAGGTCACTTCCGTCTTACCGAGAATACCCTGTGCCAGAGTACGGAACAGCGATTCGGTCAGCTCGATCAGATCTTTGTAATCTGCGTa                              <  1:2168669/93‑1 (MQ=255)

                                                           gCCGTAGGTCACTTCCGTCTTACCGAGAATACCCTGTGCCAGAGTACGGAACAGCGATTCGGTCAGCTCGATCAGATCTTTGTAATCTGCGTa                              >  1:2994293/1‑93 (MQ=255)

                                                            ccGTAGGTCACTTCCGTCTTACCGAGAATACCCTGTGCCAGAGTACGGAACAGCGATTCGGTCAGCTCGATCAGATCTTTGTAATCTGCGTaa                             <  2:6714270/93‑1 (MQ=255)

                                                               tAGGTCACTTCCGTCTTACCGAGAATACCCTGTGCCAGAGTACGGAACAGCGATTCGGTCAGCTCGATCAGATCTTTGTAATCTGCGTAAGcc                          <  1:5424422/93‑1 (MQ=255)

                                                                aGGTCACTTCCGTCTTACCGAGAATACCCTGTGCCAGAGTACGGAACAGCGATTCGGTCAGCTCGATCAGATCTTTGTAATCTGCGTAAGCCa                         <  2:359374/93‑1 (MQ=255)

                                                                aGGTCACTTCCGTCTTACCGAGAATACCCTGTGCCAGAGTACGGAACAGCGATTCGGTCAGCTCGATCAGATCTTTGTAATCTGCGTAAGCCa                         <  1:6940599/93‑1 (MQ=255)

                                                                aGGTCACTTCCGTCTTACCGAGAATACCCTGTGCCAGAGTACGGAACAGCGATTCGGTCAGCTCGATCAGATCTTTGTAATCTGCGTAAGCCa                         >  2:6594665/1‑93 (MQ=255)

                                                                 ggTCACTTCCGTCTTACCGAGAATACCCTGTGCCAGAGTACGGAACAGCGATTCGGTCAGCTCGATCAGATCTTTGTAATCTGCGTAAGCCAt                        >  2:3787739/1‑93 (MQ=255)

                                                                   tCACTTCCGTCTTACCGAGAATACCCTGTGCCAGAGTACGGAACAGCGATTCGGTCAGCTCGATCAGATCTTTGTAATCTGCGTAAGCCATGt                      <  1:3141280/93‑1 (MQ=255)

                                                                    cACTTCCGTCTTACCGAGAATGCCCTGTGCCAGAGTACGGAACAGCGATTCGGTCAGCTCGATCAGATCTTTGTAATCTGCGTAAGCCATGTa                     <  1:4103459/93‑1 (MQ=255)

                                                                      cTTCCGTCTTACCGAGAATACCCTGTGCCAGAGTACGGAACAGCGATTCGGTCAGCTCGATCAGATCTTTGTAATCTGCGTAAGCCATGTaga                   <  2:3262416/93‑1 (MQ=255)

                                                                       ttCCGTCTTACCGAGAATCCCCTGTGCCAGAGTACGGAACAGCGATTCGGTCAGCTCGATCAGATCTTTGTAATCTGCGTAAGCCATGTagag                  <  1:1027795/93‑1 (MQ=255)

                                                                        tCCGTCTTACCGAGAATACCCTGTGCCAGAGTACGGAACAGCGATTCGGTCAGCTCGATCAGATCTTTGTAATCTGCGTAAGCCATGTAGAGt                 >  2:5724669/1‑93 (MQ=255)

                                                                         ccGTCTTACCGAGAATACCCTGTGCCAGAGTACGGAACAGCGATTCGGTCAGCTCGATCAGATCTTTGTAATCTGCGTAAGCCATGTAGAGtt                >  2:4001385/1‑93 (MQ=255)

                                                                             tttcccGAGAATACCCTGTGCCAGAATACGGAACAGCGATTCGGTCAGCTCGATCAGATCTTTGTAATCTGCTTAAGCCATGTAGAGTTCcat            <  1:6298257/89‑1 (MQ=37)

                                                                             cTTACCGAGAATATCCTGTGCCAGAGTACGGAACAGCGATTCGGTCAGCTCGATCAGATCTTTGTAATCTGCGTAAGCCATGTAGAGTTCcat            >  1:7759912/1‑93 (MQ=255)

                                                                             cTTACCGAGAATACCCTGTGCCAGAGTACGGAACAGCGATTCGGTCAGCTCGATCAGATCTTTGTAATCTGCGTAAGCCATGTAGAGTTCcat            <  2:1197016/93‑1 (MQ=255)

                                                                             cTTACCGAGAATACCCTGTGCCAGAGTACGGAACAGCGATTCGGTCAGCTCAATCAGATCTTTGTAATCTGCGTAAGCCATGTAGAGTTCcat            <  2:5301805/93‑1 (MQ=255)

                                                                               tACCGAGAATACCCTGTGCCAGAGTACGGAACAGCGATTCGGTCAGCTCGATCAGATCTTTGTAATCTGCGTAAGCCATGTAGAGTTCcatca          <  1:5099427/93‑1 (MQ=255)

                                                                               tACCGAGAATACCCTGTGCCAGAGTACGGAACAGCGATTCGGTCAGCTCGATCAGATCTTTGTAATCTGCGTAAGCCATGTAGAGTTCcatca          <  2:2134698/93‑1 (MQ=255)

                                                                               tACCGAGAATACCCTGTGCCAGAGTACGGAACAGCGATTCGGTCAGCTCGATCAGATCTTTGTAATCTGCGTAAGCCATGTAGAGTTCcatca          <  2:1066872/93‑1 (MQ=255)

                                                                               tACCGAGAATACCCTGTGCCAGAGTACGGAACAGCGATTCGGTCAGCTCGATCAGATCTTTGTAATCTGCGTAAGCCATGTAGAGTTCcatca          <  2:6790376/93‑1 (MQ=255)

                                                                                 ccGAGAATACCCTGTGCCAGAGTACGGAACAGCGATTCGGTCAGCTCGATCAGATCTTTGTAATCTGCGTAAGCCATGTAGAGTTCCATCATg        <  1:595458/93‑1 (MQ=255)

                                                                                 ccGAGAATACCCTGTGCCAGAGTACGGAACAGCGATTCGGTCAGCTCGATCAGATCTTTGTAATCTGCGTAAGCCATGTAGAGTTCCATCATg        <  2:2786820/93‑1 (MQ=255)

                                                                                 ccGAGAATACCCTGTGCCAGAGTACGGAACAGCGATTCGGTCAGCTCGATCAGATCTTTGTAATCTGCGTAAGCCATGTAGAGTTCCATCATg        <  2:5569669/93‑1 (MQ=255)

                                                                                  cGAGAATACCCTGTGCCAGAGTACGGAACAGCGATTCGGTCAGCTCGATCAGATCTTTGTAATCTGCGTAAGCCATGTAGAGTTCCATCATgg       <  1:877586/93‑1 (MQ=255)

                                                                                  cGAGAATACCCTGTGCCAGAGTACGGAACAGCGATTCGGTCAGCTCGATCAGATCTTTGTAATCTGCGTAAGCCATGTAGAGTTCCATCATgg       <  1:709261/93‑1 (MQ=255)

                                                                                   gagaATACCCTGTGCCAGAGTACGGAACAGCGATTCGGTCAGCTCGATCAGATCTTTGTAATCTGCGTAAGCCATGTAGAGTTCCATCATGGt      <  1:976605/93‑1 (MQ=255)

                                                                                   gagaATACCCTGTGCCAGAGTACGGAACAGCGATTCGGTCAGCTCGATCAGATCTTTGTAATCTGCGTAAGCCATGTAGAGTTCCATCATGGt      <  2:5582535/93‑1 (MQ=255)

                                                                                    agaATACCCTGTGCCAGAGTACGGAACAGCGATTCGGTCAGCTCGATCAGATCTTTGTAATCTGCGTAAGCCATGTAGAGTTCCATCATGGTg     <  1:7252618/93‑1 (MQ=255)

                                                                                     gaATACCCTGTGCCAGAGTACGGAACAGCGATTCGGTCAGCTCGATCAGATCTTTGTAATCTGCGTAAGCCATGTAGAGTTCCATCATGGTGa    >  2:1359848/1‑93 (MQ=255)

                                                                                      aaTACCCTGTGCCAGAGTACGGAACAGCGATTCGGTCAGCTCGATCAGATCTTTGTAATCTGCGTAAGCCATGTAGAGTTCCATCATGGTGaa   >  2:766456/1‑93 (MQ=255)

                                                                                       atacCCTGTGCCAGAGTACGGAACAGCGATTCGGTCAGCTCGATCAGATCTTTGTAATCTGCGTAAGCCATGTAGAGTTCCATCATGGTGAAc  <  1:6516396/93‑1 (MQ=255)

                                                                                       atacCCTGTGCCAGAGTACGGAACAGCGATTCGGTCAGCTCGATCAGATCTTTGTAATCTGCGTAAGCCATGTAGAGTTCCATCATGGTGAAc  >  2:1829681/1‑93 (MQ=255)

TTGATCGCTTCACGCATGGTCAGTTTTTCGAACGGTTTACCGAAGTCCAGCGTCACGTCGCCGTAGGTCACTTCCGTCTTACCGAGAATATCCTGTGCCAGAGTACGGAACAGCGATTCGGTCAGCTCGATCAGATCTTTGTAATCTGCGTAAGCCATGTAGAGTTCCATCATGGTGAAC  >  NC_000913/3032194‑3032373

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 21 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`