BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,046,045	T→C	V339V (GTA→GTG)	gcvP ←	glycine decarboxylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,046,045	0	T	C	99.6%	868.1 / ‑5.4	275	V339V (GTA→GTG)	gcvP	glycine decarboxylase
Reads supporting (aligned to +/- strand): ref base T (0/1); new base C (127/146); total (127/148)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.85e-01

Only 100 of 286 total aligned reads displayed.

ATCGGTCAGACGGTGAATGCGGTTAGCGATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGTACCTGGGAAGTACAAATGTTGGAGTTCGCTTTCTCACGGCGGATATGTTGCTCGCGAGTCTGCATCGCCATGCGCAGCGCGGTATTGCC  >  NC_000913/3045955‑3046134

 tCGGTCAGACGGTGAATGCGGTTAGCGATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGCAcc                                                                                        >  1:901771/1‑93 (MQ=255)

 tCGGTCAGACGGTGAATGCGGTTAGCGATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGCAcc                                                                                        <  2:3710159/93‑1 (MQ=255)

 tCGGTCAGACGGTGAATGCGGTTAGCGATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGCAcc                                                                                        >  2:4236807/1‑93 (MQ=255)

  cGGTCAGACGGTGAATGCGGTTAGCGATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGCACCt                                                                                       <  1:3658741/93‑1 (MQ=255)

   ggTCAGACGGTGAATGCGGTTAGCGATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGCACCTg                                                                                      <  2:1596916/93‑1 (MQ=255)

   ggTCAGACGGTGAATGCGGTTAGCGATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGCACCTg                                                                                      >  2:1998996/1‑93 (MQ=255)

    gTCAGACGGTGAATGCGGTTAGCGATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGCACCtcg                                                                                     >  1:3490744/1‑91 (MQ=255)

    gTCAGACGGTGAATGCGGTTAGCGATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGCACCTgg                                                                                     <  1:2113762/93‑1 (MQ=255)

    gTCAGACGGTGAATGCGGTTAGCGATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGCACCTgg                                                                                     <  1:4671287/93‑1 (MQ=255)

       aGACGGTGAATGCGGTTAGCGATACGTTTCAGGCCAGCCGGGCCGGGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGCACCTGGGaa                                                                                  <  1:2055813/93‑1 (MQ=255)

       aGACGGTGAATGCGGTTAGCGATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGCACCTGGGaa                                                                                  <  1:1114534/93‑1 (MQ=255)

       aGACGGTGAATGCGGTTAGCGATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGCACCTGGGaa                                                                                  >  2:2971505/1‑93 (MQ=255)

       aGACGGTGAATGCGGTTAGCGATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGCACCTGGGaa                                                                                  <  2:3266970/93‑1 (MQ=255)

       aGACGGTGAATGCGGTTAGCGATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGCACCTGGGaa                                                                                  <  1:4209273/93‑1 (MQ=255)

        gACGGTGAATGCGGTTAGCGATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGCACCTGGGAAg                                                                                 >  2:68511/1‑93 (MQ=255)

           ggTGAATGCGGTTAGCGATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGCACCTGGGAAGTAc                                                                              >  2:3996353/1‑93 (MQ=255)

            gTGAATGCGGTTAGCGATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGCACCTGGGAAGTACa                                                                             <  1:7785152/93‑1 (MQ=255)

             tGAATGCGGTTAGCGATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGCACCTGGGAAGTACaa                                                                            <  2:167723/93‑1 (MQ=255)

             tGAATGCGGTTAGCGATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGCACCTGGGAAGTACaa                                                                            <  2:79440/93‑1 (MQ=255)

             tGAATGCGGTTAGCGATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGCACCTGGGAAGTACaa                                                                            >  1:1070572/1‑93 (MQ=255)

               aaTGCGGTTAGCGATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGCACCTGGGAAGTACAAAt                                                                          >  2:2994947/1‑93 (MQ=255)

                aTGCGGTTAGCGATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGCACCTGGGAAGTACAAATg                                                                         <  2:7129416/93‑1 (MQ=255)

                aTGCGGTTAGCGATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGCACCTGGGAAGTACAAATg                                                                         >  1:5452706/1‑93 (MQ=255)

                   cGGTTAGCGATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGCACCTGGGAAGTACAAATGTTg                                                                      <  1:455532/93‑1 (MQ=255)

                   cGGTTAGCGATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGCACCTGGGAAGTACAAATGTTg                                                                      <  1:736094/93‑1 (MQ=255)

                    ggTTAGCGATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGCACCTGGGAAGTACAAATGTTgg                                                                     >  1:5766032/1‑93 (MQ=255)

                    ggTTAGCGATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGCACCTGGGAAGTACAAATGTTgg                                                                     >  2:2353684/1‑93 (MQ=255)

                     gTTAGCGATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGCACCTGGGAAGTACAAATGTTGGa                                                                    <  2:7413291/93‑1 (MQ=255)

                     gTTAGCGATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGCACCTGGGAAGTACAAATGTTGGa                                                                    >  1:1919113/1‑93 (MQ=255)

                      ttAGCGATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGCACCTGGGAAGTACAAATGTTGGAg                                                                   >  1:2529822/1‑93 (MQ=255)

                        aGCGATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGCACCTGGGAAGTACAAATGTTGGAGtt                                                                 <  2:2176481/93‑1 (MQ=255)

                         gCGATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGCACCTGGGAAGTACAAATGTTGGAGTTc                                                                >  2:63297/1‑93 (MQ=255)

                         gCGATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGCACCTGGGAAGTACAAATGTTGGAGTTc                                                                <  2:3549344/93‑1 (MQ=255)

                         gCGATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGCACCTGGGAAGTACAAATGTTGGAGTTc                                                                >  1:3697126/1‑93 (MQ=255)

                          cGATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGCACCTGGGAAGTACAAATGTTGGAGTTCg                                                               <  2:6193177/93‑1 (MQ=255)

                           gATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGCACCTGGGAAGTACAAATGTTGGAGTTCGc                                                              >  1:437321/1‑93 (MQ=255)

                           gATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGCACCTGGGAAGTACAAATGTTGGAGTTCGc                                                              >  1:2306509/1‑93 (MQ=255)

                            aTACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGCACCTGGGAAGTACAAATGTTGGAGTTCGCt                                                             <  1:1370358/93‑1 (MQ=255)

                            aTACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGCACCTGGGAAGTACAAATGTTGGAGTTCGCt                                                             <  1:2685361/93‑1 (MQ=255)

                               cGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGCACCTGGGAAGTACAAATGTTGGAGTTCGCTTtc                                                          <  2:454062/93‑1 (MQ=255)

                                gTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGCACCTGGGAAGTACAAATGTTGGAGTTCGCTTtct                                                         >  2:669781/1‑93 (MQ=255)

                                gTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGCACCTGGGAAGTACAAATGTTGGAGTTCGCTTtct                                                         >  1:324210/1‑93 (MQ=255)

                                  ttCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGCACCTGGGAAGTACAAATGTTGGAGTTCGCTTTCTCa                                                       <  2:7231676/93‑1 (MQ=255)

                                   tCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGCACCTGGGAAGTACAAATGTTGGAGTTCGCTTTCTCAc                                                      <  1:2648355/93‑1 (MQ=255)

                                        ccAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGCACCTGGGAAGTACAAATGTTGGAGTTCGCTTTCTCAcggcgg                                                 <  1:5313306/93‑1 (MQ=255)

                                         cAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGCACCTGGGAAGTACAAATGTTGGAGTTCGCTTTCTCACGGCGGa                                                <  1:1575175/93‑1 (MQ=255)

                                          aaCCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGCACCTGGGAAGTACAAATGTTGGAGTTCGCTTTCTCACGGCGGat                                               <  1:6353936/93‑1 (MQ=255)

                                          aaCCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGCACCTGGGAAGTACAAATGTTGGAGTTCGCTTTCTCACGGCGGat                                               <  2:504568/93‑1 (MQ=255)

                                           aCCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGCACCTGGGAAGTACAAATGTTGGAGTTCGCTTTCTCACGGCGGata                                              <  2:731406/93‑1 (MQ=255)

                                           aCCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGCACCTGGGAAGTACAAATGTTGGAGTTCGCTTTCTCACGGCGGata                                              >  1:3969797/1‑93 (MQ=255)

                                            ccGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGCACCTGGGAAGTACAAATGTTGGAGTTCGCTTTCTCACGGCGGatat                                             <  1:4880232/93‑1 (MQ=255)

                                              gggCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGCACCTGGGAAGTACAAATGTTGGAGTTCGCTTTCTCACGGCGGATATGt                                           <  2:3868729/93‑1 (MQ=255)

                                              gggCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGCACCTGGGAAGTACAAATGTTGGAGTTCGCTTTCTCACGGCGGATATGt                                           >  2:911866/1‑93 (MQ=255)

                                               ggCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGCACCTGGGAAGTACAAATGTTGGAGTTCGCTTTCTCACGGCGGATATGtt                                          <  2:4323484/93‑1 (MQ=255)

                                               ggCAGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGCACCTGGGAAGTACAAATGTTGGAGTTCGCTTTCTCACGGCGGATATGtt                                          <  2:2744370/93‑1 (MQ=255)

                                                gCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGCACCTGGGAAGTACAAATGTTGGAGTTCGCTTTCTCACGGCGGATATGTTg                                         >  1:3365500/1‑93 (MQ=255)

                                                gCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGCACCTGGGAAGTACAAATGTTGGAGTCCGCTTTCTCACGGCGGATATGTTg                                         >  1:2856109/1‑93 (MQ=255)

                                                 gcGTGATAAACGGCATACAGGCTGGCTATGTTTGCCAGCAGCACCTGGGAAGTACAAATGTTGGAGTTCTCTTTCTCACGGCGGATATGTTGc                                        <  2:7702095/92‑1 (MQ=255)

                                                   gTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGCACCTGGGAAGTACAAATGTTGGAGTTCGCTTTCTCACGGCGGATATGTTGGTc                                      >  2:4791439/1‑93 (MQ=255)

                                                   gTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGCACCTGGGAAGTACAAATGTTGGAGTTCGCTTTCTCACGGCGGATATGTTGCTc                                      >  1:96510/1‑93 (MQ=255)

                                                   gTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGCACCTGGGAAGTACAAATGTTGGAGTTCGCTTTCTCACGGCGGATATGTTGCTc                                      >  2:5645001/1‑93 (MQ=255)

                                                   gTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGCACCTGGGAAGTACAAATGTTGGAGTTCGCTTTCTCACGGCGGATATGTTGCTc                                      >  1:5625056/1‑93 (MQ=255)

                                                       tAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGCACCTGGGAAGTACAAATGTTGGAGTTCGCTTTCTCACGGCGGATATGTTGCTCGCGa                                  >  2:1193291/1‑93 (MQ=255)

                                                       tAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGCACCTGGGAAGTACAAATGTTGGAGTTCGCTTTCTCACGGCGGATATGTTGCTCGCGa                                  <  1:1297997/93‑1 (MQ=255)

                                                       tAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGCACCTGGGAAGTACAAATGTTGGAGTTCGCTTTCTCACGGCGGATATGTTGCTCGCGa                                  <  1:6732518/93‑1 (MQ=255)

                                                         aaCGGCATACAGGCTGGCGATGTTTGCCAGCAGCACCTGGGAAGTACAAATGTTGGAGTTCGCTTTCTCACGGCGGATATGTTGCTCGCGAGt                                >  2:3151671/1‑93 (MQ=255)

                                                         aaCGGCATACAGGCTGGCGATGTTTGCCAGCAGCACCTGGGAAGTACAAATGTTGGAGTTCGCTTTCTCACGGCGGATATGTTGCTCGCGAGt                                >  2:189339/1‑93 (MQ=255)

                                                         aaCGGCATACAGGCTGGCGATGTTTGCCAGCAGCACCTGGGAAGTACAAATGTTGGAGTTCGCTTTCTCACGGCGGATATGTTGCTCGCGAGt                                <  2:6385897/93‑1 (MQ=255)

                                                          aCGGCATACAGGCTGGCGATGTTTGCCAGCAGCACCTGGGAAGTACAAATGTTGGAGTTCGCTTTCTCACGGCGGATATGTTGCTCGCGAGTc                               <  2:5675840/93‑1 (MQ=255)

                                                          aCGGCATACAGGCTGGCGATGTTTGCCAGCAGCACCTGGGAAGTACAAATGTTGGAGTTCGCTTTCTCACGGCGGATATGTTGCTCGCGAGTc                               <  2:5860804/93‑1 (MQ=255)

                                                          aCGGCATACAGGCTGGCGATGTTTGCCAGCAGCACCTGGGAAGTACAAATGTTGGAGTTCGCTTTCTCACGGCGGAAATGTTGTTTGCGAGTc                               >  2:5115647/1‑93 (MQ=255)

                                                           cGGCATACAGGCTGGCGATGTTTGCCAGCAGCACCTGGGAAGTACAAATGTTGGAGTTCGCTTTCTCACGGCGGATATGTTGCTCGCGAGTCt                              <  2:3950869/93‑1 (MQ=255)

                                                             gCATACAGGCTGGCGATGTTTGCCAGCAGCACCTGGGAAGTACAAATGTTGGAGTTCGCTTTCTCACGGCGGATATGTTGCTCGCGAGTCTGc                            <  1:3130462/93‑1 (MQ=255)

                                                               aTACAGGCTGGCGATGTTTGCCAGCAGCACCTGGGAAGTACAAATGTTGGAGTTCGCTTTCTCACGGCGGATATGTTGCTCGCGAGTCTGCAt                          <  1:3279214/93‑1 (MQ=255)

                                                               aTACAGGCTGGCGATGTTTGCCAGCAGCACCTGGGAAGTACAAATGTTGGAGTTCGCTTTCTCACGGCGGATATGTTGCTCGCGAGTCTGCAt                          >  1:7711171/1‑93 (MQ=255)

                                                                tACAGGCTGGCGATGTTTGCCAGCAGCACCTGGGAAGTACAAATGTTGGAGTTCGCTTTCTCACGGCGGATATGTTGCTCGCGAGTCTGCATc                         >  1:6790021/1‑93 (MQ=255)

                                                                tACAGGCTGGCGATGTTTGCCAGCAGCACCTGGGAAGTACAAATGTTGGAGTTCGCTTTCTCACGGCGGATATGTTGCTCGCGAGTCTGCATc                         <  1:2434109/93‑1 (MQ=255)

                                                                tACAGGCTGGCGATGTTTGCCAGCAGCACCTGGGAAGTACAAATGTTGGAGTTCGCTTTCTCACGGCGGATATGTTGCTCGCGAGTCTGCATc                         <  1:5915057/93‑1 (MQ=255)

                                                                   aGGCTGGCGATGTTTGCCAGCAGCACCTGGGAAGTACAAATGTTGGAGTTCGCTTTCTCACGGCGGATATGTTGCTCGCGAGTCTGCATCGcc                      >  1:7324787/1‑93 (MQ=255)

                                                                    ggCTGGCGATGTTTGCCAGCAGCACCTGGGAAGTACAAATGTTGGAGTTCGCTTTCTCACGGCGGATATGTTGCTCGCGAGTCTGCATCGCCa                     <  1:5568144/93‑1 (MQ=255)

                                                                    ggCTGGCGATGTTTGCCAGCAGCACCTGGGAAGTACAAATGTTGGAGTTCGCTTTCTCACGGCGGATATGTTGCTCGCGAGTCTGCATCGCCa                     >  2:1040922/1‑93 (MQ=255)

                                                                    ggCTGGCGATGTTTGCCAGCAGCACCTGGGAAGTACAAATGTTGGAGTTCGCTTTCTCACGGCGGATATGTTGCTCGCGAGTCTGCATCGCCa                     >  1:6509748/1‑93 (MQ=255)

                                                                     gCTGGCGATGTTTGCCAGCAGCACCTGGGAAGTACAAATGTTGGAGTTCGCTTTCTCACGGCGGATATGTTGCTCGCGAGTCTGCATCGCCAt                    >  2:2491820/1‑93 (MQ=255)

                                                                      cTGGCGATGTTTGCCAGCAGCACCTGGGAAGTACAAATGTTGGAGTTCGCTTTCTCACGGCGGATATGTTGCTCGCGAGTCTGCATCGCCATg                   <  2:3664722/93‑1 (MQ=255)

                                                                          cGATGTTTGCCAGCAGCACCTGGGAAGTACAAATGTTGGAGTTCGCTTTCTCACGGCGGATATGTTGCTCGCGAGTCTGCATCGCCATGCGCa               <  1:814252/93‑1 (MQ=255)

                                                                           gATGTTTGCCAGCAGCACCTGGGAAGTACAAATGTTGGAGTTCGCTTTCTCACGGCGGATATGTTGCTCGCGAGTCTGCATCGCCATGCGCAg              >  1:2620205/1‑93 (MQ=255)

                                                                            aTGTTTGCCAGCAGCACCTGGGAAGTACAAATGTTGGAGTTCGCTTTCTCACGGCGGATATGTTGCTCGCGAGTCTGCATCGCCATGCGCAgc             <  1:194933/93‑1 (MQ=255)

                                                                            aTGTTTGCCAGCAGCACCTGGGAAGTACAAATGTTGGAGTTCGCTTTCTCACGGCGGATATGTTGCTCGCGAGTCTGCATCGCCATGCGCAgc             >  2:2701436/1‑93 (MQ=255)

                                                                            aTGTTTGCCAGCAGCACCTGGGAAGTACAAATGTTGGAGTTCGCTTTCTCACGGCGGATATGTTGCTCGCGAGTCTGCATCGCCATGCGCAgc             >  1:5079898/1‑93 (MQ=255)

                                                                            aTGTTTGCCAGCAGCACCTGGGAAGTACAAATGTTGGAGTTCGCTTTCTCACGGCGGATATGTTGCTCGCGAGTCTGCATCGCCATGCGCAgc             >  2:5022507/1‑93 (MQ=255)

                                                                             tGTTTGCCAGCAGCACCTGGGAAGTACAAATGTTGGAGTTCGCTTTCTCACGGCGGATATGTTGCTCGCGAGTCTGCATCGCCATGCGCcgcg            >  2:7007987/1‑93 (MQ=255)

                                                                             tGTTTGCCAGCAGCACCTGGGAAGTACAAATGTTGGAGTTCGCTTTCTCACGGCGGATATGTTGCTCGCGAGTCTGCATCGCCATGCGCAgcg            >  2:7176708/1‑93 (MQ=255)

                                                                               tttGCCAGCAGCACCTGGGAAGTACAAATGTTGGAGTTCGCTTTCTCACGGCGGATATGTTGCTCGCGAGTCTGCATCGCCATGCGCAGcgcg          <  1:5172523/93‑1 (MQ=255)

                                                                               tttGCCAGCAGCACCTGGGAAGTACAAATGTTGGAGTTCGCTTTCTCACGGCGGATATGTTGCTCGCGAGTCTGCATCGCCATGCGCAGcgcg          >  2:7244529/1‑93 (MQ=255)

                                                                                  gCCAGCAGCACCTGGGAAGTACAAATGTTGGAGTTCGCTTTCTCACGGCGGATATGTTGCTCGCGAGTCTGCATCGCCATGCGCAGCGCGGTa       >  1:6083535/1‑93 (MQ=255)

                                                                                  gCCAGCAGCACCTGGGAAGTACAAATGTTGGAGTTCGCTTTCTCACGGCGGATATGTTGCTCGCGAGTCTGCATCGCCATGCGCAGCGCGGTa       <  1:6168492/93‑1 (MQ=255)

                                                                                    cagcagCACCTGGGAAGTACAAATGTTGGAGTTCGCTTTCTCACGGAGGATATGTTGCTCGCGAGTCTGCATCGCCATGCGCAGCGCGGTAtt     >  1:2392045/1‑93 (MQ=255)

                                                                                     agcagcACCTGGGAAGTACAAATGTTGGAGTTCGCTTTCTTACGGCGGATATGTTGCTCGCGAGTCTGCATCGCCATGCGCAGCGCGGTATTg    >  2:7658005/1‑93 (MQ=255)

                                                                                      gcagcaCCTGGGAAGTACAAATGTTGGAGTTCGCTTTCTCACGGCGGATATGTTGCTCGCGAGTCTGCATCGCCATGCGCAGCGCGGTATTGc   >  2:25683/1‑93 (MQ=255)

                                                                                      gcagcaCCTGGGAAGTACAAATGTTGGAGTTCGCTTTCTCACGGCGGATATGTTGCTCGCGAGTCTGCATCGCCATGCGCAGCGCGGTATTGc   >  2:1409832/1‑93 (MQ=255)

ATCGGTCAGACGGTGAATGCGGTTAGCGATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGTACCTGGGAAGTACAAATGTTGGAGTTCGCTTTCTCACGGCGGATATGTTGCTCGCGAGTCTGCATCGCCATGCGCAGCGCGGTATTGCC  >  NC_000913/3045955‑3046134

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`