BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,047,558	T→C	V4V (GTA→GTG)	gcvH ←	GcvH

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,047,558	0	T	C	99.7%	1151.0 / NA	366	V4V (GTA→GTG)	gcvH	GcvH
Reads supporting (aligned to +/- strand): ref base T (0/1); new base C (204/160); total (204/162)
Fisher's exact test for biased strand distribution p-value = 4.41e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 371 total aligned reads displayed.

CTGAGCATGTTCGGTAATACCAACGGTGTAAGTGCCGTCGGCTTCTTTACGCAGCCATTCGTGTTCTTTGCTGTATTTCAGTTCTGCTGGTACGTTGCTCATCAATCAATCTCCAAAAAAGTAAATCACGCGACGGCTTTGCCGTTACGCACAAAAACAGGTTTTGTCACTTTAACCGGC  >  NC_000913/3047468‑3047647

cTGAGCATGTTCGGTAATACCAACGGTGTAAGTGCCGTCGGCTTCTTTACGCAGCCATTCGTGTTCTTTGCTGTATTTCAGTTCTGCTGGCAc                                                                                         >  2:4054106/1‑93 (MQ=255)

   aGCATGTTCGGTAATACCAACGGTGTAAGTGCCGTCGGCTTCTTTACGCAGCCATTCGTGTTCTTTGCTGTATTTCAGTTCTGCTGGCACGtt                                                                                      <  2:5862387/93‑1 (MQ=255)

   aGCATGTTCGGTAATACCAACGGTGTAAGTGCCGTCGGCTTCTTTACGCAGCCATTCGTGTTCTTTGCTGTATTTCAGTTCTGCTGGCACGtt                                                                                      <  2:3097114/93‑1 (MQ=255)

    gCATGTTCGGTAATACCAACGGTGTAAGTGCCGTCGGCTTCTTTACGCAGCCATTCGTGTTCTTTGCTGAATTTCAGTTCTGCTGGCACGTTg                                                                                     >  2:5662441/1‑93 (MQ=255)

     cATGTTCGGTAATACCAACGGTGTAAGTGCCGTCGGCTTCTTTACGCAGCCATTCGTGTTCTTTGCTGTATTTCAGTTCTGCTGGCACGTTGc                                                                                    >  2:2528982/1‑93 (MQ=255)

       tGTTCGGTAATACCAACGGTGTAAGTGCCGTCGGCTTCTTTACGCAGCCATTCGTGTTCTTTGCTGTATTTCAGTTCTGCTGGCACGTTGCtc                                                                                  >  1:2414992/1‑93 (MQ=255)

       tGTTCGGTAATACCAACGGTGTAAGTGCCGTCGGCTTCTTTACGCAGCCATTCGTGTTCTTTGCTGTATTTCAGTTCTGCTGGCACGTTGCtc                                                                                  >  1:4793118/1‑93 (MQ=255)

        tttCGGTAATACCAACGGTGTAAGTGCCGTCGGCTTCTTTACGCAGCCATTCGTGTTCTTTGCTGTATTTCAGTTCTGCTGGCACGTTGCtca                                                                                 <  1:77500/92‑1 (MQ=255)

        gTTCGGTAATACCAACGGTGTAAGTGCCGTCGGCTTCTTTACGCAGCCATTCGTGTTCTTTGCTGTATTTCAGTTCTGCTGGCACGTTGCtca                                                                                 >  1:4166171/1‑93 (MQ=255)

        gTTCGGTAATACCAACGGTGTAAGTGCCGTCGGCTTCTTTACGCAGCCATTCGTGTTCTTTGCTGTATTTCAGTTCTGCTGGCACGTTGCtca                                                                                 <  2:7202372/93‑1 (MQ=255)

         ttCCGTAATACCAACGGTGTAAGTGCCGTCGGCTTCTTTACGCAGCCATTCGTGTTCTTTGCTGTATTTCAGTTCTGCTGGCACGTTGCtcat                                                                                <  1:5986686/93‑1 (MQ=255)

          tCGGTAATACCAACGGTGTAAGTGCCGTCGGCTTCTTTACGCAGCCATTCGTGTTCTTTGCTGTATTTCAGTTCTGCTGGCACGTTGCtcatc                                                                               <  1:2095200/93‑1 (MQ=255)

          tCGGTAATACCAACGGTGTAAGTGCCGTCGGCTTCTTTACGCAGCCATTCGTGTTCTTTGCTGTATTTCAGTTCTGCTGGCACGTTGCtcatc                                                                               <  1:6176776/93‑1 (MQ=255)

           cGGTAATACCAACGGTGTAAGTGCCGTCGGCTTCTTTACGCAGCCATTCGTGTTCTTTGCTGTATTTCAGTTCTGCTGGCACGTTGCtcatca                                                                              >  1:324710/1‑93 (MQ=255)

            ggTAATACCAACGGTGTAAGTGCCGTCGGCTTCTTTACGCAGCCATTCGTGTTCTTTGCTGTATTTCAGTTCTGCTGGCACGTTGCTCatcaa                                                                             >  1:6761043/1‑93 (MQ=255)

             gTAATACCAACGGTGTAAGTGCCGTCGGCTTCTTTACGCAGCCATTCGTGTTCTTTGCTGTATTTCAGTTCTGCTGGCACGTTGCTCatcaat                                                                            >  2:4246982/1‑93 (MQ=255)

             gTAATACCAACGGTGTAAGTGCCGTCGGCTTCTTTACGCAGCCATTCGTGTTCTTTGCTGTATTTCAGTTCTGCTGGCACGTTGCTCatcaat                                                                            >  2:777542/1‑93 (MQ=255)

              tAATACCAACGGTGTAAGTGCCGTCGGCTTCTTTACGCAGCCATTCGTGTTCTTTGCTGTATTTCAGTTCTGCTGGCACGTTGCTCatcaatc                                                                           <  2:3015511/93‑1 (MQ=255)

                 tACCAACGGTGTAAGTGCCGTCGGCTTCTTTACGCAGCCATTCGTGTTCTTTGCTGTATTTCAGTTCTGCTTGCACGTTGTTCCTccattaat                                                                        >  2:1454264/1‑93 (MQ=255)

                 tACCAACGGTGTAAGTGCCGTCGGCTTCTTTACGCAGCCATTCGTGTTCTTTGCTGTATTTCAGTTCTGCTGGCACGTTGCTCATcaatcaat                                                                        >  2:7887861/1‑93 (MQ=255)

                 tACCAACGGTGTAAGTGCCGTCGGCTTCTTTACGCAGCCATTCGTGTTCTTTGCTGTATTTCAGTTCTGCTGGCACGTTGCTCATcaatcaat                                                                        <  1:1753187/93‑1 (MQ=255)

                 tACCAACGGTGTAAGTGCCGTCGGCTTCTTTACGCAGCCATTCGTGTTCTTTGCTGTATTTCAGTTCTGCTGGCACGTTGCTCATcaatcaat                                                                        >  1:2246700/1‑93 (MQ=255)

                 tACCAACGGTGTAAGTGCCGTCGGCTTCTTTACGCAGCCATTCGTGTTCTTTGCTGTATTTCAGTTCTGCTGGCACGTTGCTCATcaatcaat                                                                        <  2:5235634/93‑1 (MQ=255)

                  aCCAACGGTGTAAGTGCCGTCGGCTTCTTTACGCAGCCATTCGTGTTCTTTGCTGTATTTCAGTTCTGCTGGCACGTTGCTCATCaatcaatc                                                                       <  2:1932493/93‑1 (MQ=255)

                    cAACGGTGTAAGTGCCGTCGGCTTCTTTACGCAGCCATTCGTGTTCTTTGCTGTATTTCAGTTCTGCTGGCACGTTGCTCATCAATCAAtctc                                                                     >  2:2129177/1‑93 (MQ=255)

                     aaCGGTGTAAGTGCCGTCGGCTTCTTTACGCAGCCATTCGTGTTCTTTGCTGTATTTCAGTTCTGCTGGCACGTTGCTCATCAATCAATCTcc                                                                    >  1:5079800/1‑93 (MQ=255)

                       cGGTGTAAGTGCCGTCGGCTTCTTTACGCAGCCATTCGTGTTCTTTGCTGTATTTCAGTTCTGCTGGCACGTTGCTCATCAATCAATCTCCaa                                                                  <  1:5265631/93‑1 (MQ=255)

                         gtgtAAGTGCCGTCGGCTTCTTTACGCAGCCATTCGTGTTCTTTGCTGTATTTCAGTTCTGCTGGCACGTTGCTCATCAATCAATCTCCaaaa                                                                >  1:6307048/1‑93 (MQ=255)

                          tgtAAGTGCCGTCGGCTTCTTTACGCAGCCATTCGTGTTCTTTGCTGTATTTCAGTTCTGCTGGCACGTTGCTCATCAATCAATCTCCaaaaa                                                               >  1:53176/1‑93 (MQ=255)

                             aaGTGCCGTCGGCTTCTTTACGCAGCCATTCGTGTTCTTTGCTGTATTTCAGTTCTGCTGGCACGTTGCTCATCAATCAATCTCCAAAAAAGt                                                            >  1:4861962/1‑93 (MQ=255)

                               gTGCCGTCGGCTTCTTTACGCAGCCATTCGTGTTCTTTGCTGTATTTCAGTTCTGCTGGCACGTTGCTCATCAATCAATCTCCAAAAAAGTaa                                                          <  2:6647701/93‑1 (MQ=255)

                                tGCCGTCGGCTTCTTTACGCAGCCATTCGTGTTCTTTGCTGTATTTCAGTTCTGCTGGCACGTTGCTCATCAATCAATCTCCAAAAAAGTaaa                                                         <  1:2775451/93‑1 (MQ=255)

                                 gCCGTCGGCTTCTTTACGCAGCCATTCGTGTTCTTTGCTGTATTTCAGTTCTGCTGGCACGTTGCTCATCAATCAATCTCCAAAAAAGTAAAt                                                        >  1:3960588/1‑93 (MQ=255)

                                  ccGTCGGCTTCTTTACGCAGCCATTCGTGTTCTTTGCTGTATTTCAGTTCTGCTGGCACGTTGCTCATCAATCAATCTCCAAAAAAGTAAATc                                                       >  2:2750017/1‑93 (MQ=255)

                                  ccGTCGGCTTCTTTACGCAGCCATTCGTGTTCTTTGCTGTATTTCAGTTCTGCTGGCACGTTGCTCATCAATCAATCTCCAAAAAAGTAAATc                                                       >  1:7076404/1‑93 (MQ=255)

                                   cGTCGGCTTCTTTACGCAGCCATTCGTGTTCTTTGCTGTATTTCAGTTCTGCTGGCACGTTGCTCATCAATCAATCTCCAAAAAAGTAAATCa                                                      <  2:3837511/93‑1 (MQ=255)

                                     ttggCTTCTTTACGCAGCCATTCGTGTTCTTTGCTGTATTTCAGTTCTGCTGGCACGTTGCTCATCAATCAATCTCCAAAAAAGTAAATCAcg                                                    >  2:4903400/3‑93 (MQ=255)

                                     tCGGCTTCTTTACGCAGCCATTCGTGTTCTTTGCTGTATTTCAGTTCTGCTGGCACGTTGCTCATCAATCAATCTCCAAAAAAGTAAATCAcg                                                    >  1:6623988/1‑93 (MQ=255)

                                      cGGCTTCTTTACGCAGCCATTCGTGTTCTTTGCTGTATTTCAGTTCTGCTGGCACGTTGCTCATCAATCAATCTCCAAAAAAGTAAATCAcgc                                                   >  2:6364307/1‑93 (MQ=255)

                                      cGGCTTCTTTACGCAGCCATTCGTGTTCTTTGCTGTATTTCAGTTCTGCTGGCACGTTGCTCATCAATCAATCTCCAAAAAAGTAAATCAcgc                                                   <  1:1817620/93‑1 (MQ=255)

                                      cGGCTTCTTTACGCAGCCATTCGTGTTCTTTGCTGTATTTCAGTTCTGCTGGCACGTTGCTCATCAATCAATCTCCAAAAAAGTAAATCAcgc                                                   <  2:2792688/93‑1 (MQ=255)

                                      cGGCTTCTTTACGCAGCCATTCGTGTTATTTGCTGTATTTCAGTTCTGCTGGCACGTTGCTCATCAATCAATCTCCAAAAAAGTAAATCAcgc                                                   >  2:646049/1‑93 (MQ=255)

                                       ggCTTCTTTACGCAGCCATTCGTGTTCTTTGCTGTATTTCAGTTCTGCTGGCACGTTGCTCATCAATCAATCTCCAAAAAAGTAAATCAcgcg                                                  >  1:4663898/1‑93 (MQ=255)

                                         cttcttTACGCAGCCATTCGTGTTCTTTGCTGTATTTCAGTTCTGCTGGCACGTTGCTCATCAATCAATCTCCAAAAAAGTAAATCACGCGAc                                                >  2:7148722/1‑93 (MQ=255)

                                         cttcttTACGCAGCCATTCGTGTTCTTTGCTGTATTTCAGTTCTGCTGGCACGTTGCTCATCAATCAATCTCCAAAAAAGTAAATCACGCGAc                                                <  1:589231/93‑1 (MQ=255)

                                          ttcttTACGCAGCCATTCGTGTTCTTTGCTGTATTTCAGTTCTGCTGGCACGTTGCTCATCAATCAATCTCCAAAAAAGTAAATCACGCGACg                                               >  2:3695760/1‑93 (MQ=255)

                                          ttcttTACGCAGCCATTCGTGTTCTTTGCTGTATTTCAGTTCTGCTGGCACGTTGCTCATCAATCAATCTCCAAAAAAGTAAATCACGCGACg                                               >  2:3940080/1‑93 (MQ=255)

                                          ttcttTACGCAGCCATTCGTGTTCTTTGCTGTATTTCAGTTCTGCTGGCACGTTGCTCATCAATCAATCTCCAAAAAAGTAAATCACGCGACg                                               >  1:5479312/1‑93 (MQ=255)

                                           tcttTACGCAGCCATTCGTGTTCTTTGCTGTATTTCAGTTCTGCTGGCACGTTGCTCATCAATCAATCTCCAAAAAAGTAAATCACGCGACgg                                              >  2:1000203/1‑93 (MQ=255)

                                           tcttTACGCAGCCATTCGTGTTCTTTGCTGTATTTCAGTTCTGCTGGCACGTTGCTCATCAATCAATCTCCAAAAAAGTAAATCACGCGACgg                                              >  1:388261/1‑93 (MQ=255)

                                              ttACGCAGCCATTCGTGTCCTTTGCTGTATTTCAGTTCTGCTGGCACGTTGCTCATCAATCAATCTCCAAAAAAGTAAATCACGCGACGGCtt                                           <  2:1354288/93‑1 (MQ=255)

                                                aCGCAGCCATTCGTGTTCTTTGCTGTATTTCAGTTCTGCTGGCACGTTGCTCATCAATCAATCTCCAAAAAAGTAAATCACGCGACGGCTTTg                                         >  1:1197810/1‑93 (MQ=255)

                                                aCGCAGCCATTCGTGTTCTTTGCTGTATTTCAGTTCTGCTGGCACGTTGCTCATCAATCAATCTCCAAAAAAGTAAATCACGCGACGGCTTTg                                         >  1:4980761/1‑93 (MQ=255)

                                                aCGCAGCCATTCGTGTTCTTTGCTGTATTTCAGTTCTGCTGGCACGTTGCTCATCAATCAATCTCCAAAAAAGTAAATCACGCGACGGCTTTg                                         <  2:2950394/93‑1 (MQ=255)

                                                 cGCAGCCATTCGTGTTCTTTGCTGTATTTCAGTTCTGCTGGCACGTTGCTCATCAATCAATCTCCAAAAAAGTAAATCACGCGACGGCTTTGc                                        <  2:511949/93‑1 (MQ=255)

                                                   cAGCCATTCGTGTTCTTTGCTGTATTTCAGTTCTGCTGGCACGTTGCTCATCAATCAATCTCCAAAAAAGTAAATCACGCGACGGCTTTGCCg                                      <  2:4720086/93‑1 (MQ=255)

                                                    aGCCATTCGTGTTCTTTGCTGTATTTCAGTTCTGCTGGCACGTTGCTCATCAATCAATCTCCAAAAAAGTAAATCACGCGACGGCTTTGCCGt                                     <  2:5461859/93‑1 (MQ=255)

                                                     gCCATTCGTGTTCTTTGCTGTATTTCAGTTCTGCTTGCACGTTGCTCATCAATCAATCTCCAAAAAAGTAAATCACGCGACGGCTTTGCCGtt                                    >  1:1385510/1‑93 (MQ=255)

                                                     gCCATTCGTGTTCTTTGCTGTATTTCAGTTCTGCTGGCACGTTGCTCATCAATCAATCTCCAAAAAAGTAAATCACGCGACGGCTTTGCCGtt                                    <  2:114249/93‑1 (MQ=255)

                                                      ccATTCGTGTTCCTTGCTGTATTTCAGTTCTGCTGGCACAGTGCTCATCAATCAATCTCCAAAAAAGTAAATCACGCGACGTCTTTGCCGTTa                                   >  2:556263/1‑93 (MQ=255)

                                                       cATTCGTGTTCTTTGCTGTATTTCAGTTCTGCTGGCACGTTGCTCATCAATCAATCTCCAAAAAAGTAAATCACGCGACGGCTTTGCCGTTAc                                  <  1:3700133/93‑1 (MQ=255)

                                                       cATTCGTGTTCTTTGCTGTATTTCAGTTCTGCTGGCACGTTGCTCATCAATCAATCTCCAAAAAAGTAAATCACGCGACGGCTTTGCCGTTAc                                  <  2:6221287/93‑1 (MQ=255)

                                                       cATTCGTGTTCTTTGCTGTATTTCAGTTCTGCTGGCACGTTGCTCATCAATCAATCTCCAAAAAAGTAAATCACGCGACGGCTTTGCCGTTAc                                  >  2:2655114/1‑93 (MQ=255)

                                                         ttCGTGTTCTTTGCTGTATTTCAGTTCTGCTGGCACGTTGCTCATCAATCAATCTCCAAAAAAGTAAATCACGCGACGGCTTTGCCGTTACGc                                >  1:7451303/1‑93 (MQ=255)

                                                         ttCGTGTTCTTTGCTGTATTTCAGTTCTGCTGGCACGTTGCTCATCAATCAATCTCCAAAAAAGTAAATCACGCGACGGCTTTGCCGTTACGc                                >  2:5756985/1‑93 (MQ=255)

                                                          tCGTGTTCTTTGCTGTATTTCAGTTCTGCTGGCACGTTGCTCATCAATCAATCTCCAAAAAAGTAAATCACGCGACGGCTTTGCCGTTACGca                               >  1:1962148/1‑93 (MQ=255)

                                                          tCGTGTTCTTTGCTGTATTTCAGGTCTGCTGGCACGTTGCTCATCAATCAATCTCCAAAAAAGTAAATCACGCGACGGCTTTGCCGTTACGca                               <  2:4282357/93‑1 (MQ=255)

                                                            gtgtTCTTTGCTGTATTTCAGTTCTGCTGGCACGTTGCTCATCAATCAATCTCCAAAAAAGTAAATCACGCGACGGCTTTGCCGTTACGcaca                             >  1:7593216/1‑93 (MQ=255)

                                                            gtgtTCTTTGCTGTATTTCAGTTCTGCTGGCACGTTGCTCATCAATCAATCTCCAAAAAAGTAAATCACGCGACGGCTTTGCCGTTACGcaca                             >  1:4090143/1‑93 (MQ=255)

                                                              gtTCTTTGCTGTATTTCAGTTCTGCTGGCACGTTGCTCATCAATCAATCTCCAAAAAAGTAAATCACGCGACGGCTTTGCCGTTACGCACaaa                           >  1:6588063/1‑93 (MQ=255)

                                                              gtTCTTTGCTGTATTTCAGTTCTGCTGGCACGTTGCTCATCAATCAATCTCCAAAAAAGTAAATCACGCGACGGCTTTGCCGTTACGCACaaa                           <  1:2967901/93‑1 (MQ=255)

                                                               ttCTTTGCTGTATTTCAGTTCTGCTGGCACGTTGCTCATCAATCAATCTCCAAAAAAGTAAATCACGCGACGGCTTTGCCGTTACGCACaaaa                          >  2:1979966/1‑93 (MQ=255)

                                                               ttCTTTGCTGTATTTCAGTTCTGCTGGCACGTTGCTCATCAATCAATCTCCAAAAAAGTAAATCACGCGACGGCTTTGCCGTTACGCACaaaa                          >  2:1397111/1‑93 (MQ=255)

                                                                  tttGCTGTATTTCAGTTCTGCTGGCACGTTGCTCATCAATCAATCTCCAAAAAAGTAAATCACGCGACGGCTTTGCCGTTACGCACAAAAACa                       <  1:1032035/93‑1 (MQ=255)

                                                                     gCTGTATTTCAGTTCTGCTGGCACGTTGCTCATCAATCAATCTCCAAAAAAGTAAATCACGCGACGGCTTTGCCGTTACGCACAAAAACAGGt                    <  1:45307/93‑1 (MQ=255)

                                                                     gCTGTATTTCAGTTCTGCTGGCACGTTGCTCATCAATCAATCTCCAAAAAAGTAAATCACGCGACGGCTTTGCCGTTACGCACAAAAACAGGt                    <  1:3598848/93‑1 (MQ=255)

                                                                        gTATTTCAGTTCTGCTGGCACGTTGCTCATCAATCAATCTCCAAAAAAGTAAATCACGCGACGGCTTTGCCGTTACGCACAAAAACAGGtttt                 >  2:7479853/1‑93 (MQ=255)

                                                                         tATTTCAGTTCTGCTGGCACGTTGCTCATCAATCAATCTCCAAAAAAGTAAATCACGCGACGGCTTTGCCGTTACGCACAAAAACAGGTTTTg                >  2:2436581/1‑93 (MQ=255)

                                                                         tATTTCAGTTCTGCTGGCACGTTGCTCATCAATCAATCTCCAAAAAAGTAAATCACGCGACGGCTTTGCCGTTACGCACAAAAACAGGTTTTg                >  2:6744249/1‑93 (MQ=255)

                                                                           tttCAGTTCTGCTGGCACGTTGCTCATCAATCAATCTCCAAAAAAGTAAATCACGCGACGGCTTTGCCGTTACGCACAAAAACAGGTTTTGTc              >  1:7626406/1‑93 (MQ=255)

                                                                           tttCAGTTCTGCTGGCACGTTGCTCATCAATCAATCTCCAAAAAAGTAAATCACGCGACGGATTTGCCGTTACGCACAAAAACAGGTTTTGTc              >  1:791695/1‑93 (MQ=255)

                                                                            ttCAGTTCTGCTGGCACGTTGCTCATCAATCAATCTCCAAAAAAGTAAATCACGCGACGGCTTTGCCGTTACGCACAAAAACAGGTTTTGTCa             >  1:5201401/1‑93 (MQ=255)

                                                                             tCAGTTCTGCTGGTACGTTGCTCATCAATCAATCTCCAAAAAAGTAAATCACGCGACGGCTTTGCCGTTACGCACAAAAACAGGTTTTGTCAc            <  2:3271245/93‑1 (MQ=255)

                                                                             tCAGTTCTGCTGGCACGTTGCTCATCAATCAATCTCCAAAAAAGTAAATCACGCGACGGCTTTGCCGTTACGCACAAAAACAGGTTTTGTCAc            <  2:260976/93‑1 (MQ=255)

                                                                               aGTTCTGCTGGCACGTTGCTCATCAATCAATCTCCAAAAAAGTAAATCACGCGACGGCTTTGCCGTTACGCACAAAAACAGGTTTTGTCACtt          >  2:6098336/1‑93 (MQ=255)

                                                                                gTTCTGCTGGCACGTTGCTCATCAATCAATCTCCAAAAAAGTAAATCACGCGACGGCTTTGCCGTTACGCACAAAAACAGGTTTTGTCACttt         >  2:6825630/1‑93 (MQ=255)

                                                                                  tCTGCTGGCACGTTGCTCATCAATCAATCTCCAAAAAAGTAAATCACGCGACGGCTTTGCCGTTACGCACAAAAACAGGTTTTGTCACTTTaa       >  2:4620066/1‑93 (MQ=255)

                                                                                  tCTGCTGGCACGTTGCTCATCAATCAATCTCCAAAAAAGTAAATCACGCGACGGCTTTGCCGTTACGCACAAAAACAGGTTTTGTCACTTTaa       <  2:4398622/93‑1 (MQ=255)

                                                                                   ctgctgGCACGTTGCTCATCAATCAATCTCCAAAAAAGTAAATCACGCGACGGCTTTGCCGTTACGCACAAATACAGGTTTTGTCACTTTAAc      <  2:7613951/93‑1 (MQ=255)

                                                                                   ctgctgGCACGTTGCTCATCAATCAATCTCCAAAAAAGTAAATCACGCGACGGCTTTGCCGTTACGCACAAAAACAGGTTTTGTCACTTTAAc      >  1:4325918/1‑93 (MQ=255)

                                                                                   ctgctgGCACGTTGCTCATCAATCAATCTCCAAAAAAGTAAATCACGCGACGGCTTTGCCGTTACGCACAAAAACAGGTTTTGTCACTTTAAc      >  1:3423586/1‑93 (MQ=255)

                                                                                   ctgctgGCACGTTGCTCATCAATCAATCTCCAAAAAAGTAAATCACGCGACGGCTTTGCCGTTACGCACAAAAACAGGTTTTGTCACTTTAAc      <  2:5309307/93‑1 (MQ=255)

                                                                                   ctgctgGCACGTTGCTCATCAATCAATCTCCAAAAAAGTAAATCACGCGACGGCTTTGCCGTTACGCACAAAAACAGGTTTTGTCACTTTAAc      <  1:5397995/93‑1 (MQ=255)

                                                                                    tgctgGCACGTTGCTCATCAATCAATCTCCAAAAAAGTAAATCACGCGACGGCTTTGCCGTTACGCACAAAAACAGGTTTTGTCACTTTAAcc     >  2:3343086/1‑93 (MQ=255)

                                                                                    tgctgGCACGTTGCTCATCAATCAATCTCCAAAAAAGTAAATCACGCGACGGCTTTGCCGTTACGCACAAAAACAGGTTTTGTCACTTTAAcc     >  1:6105261/1‑93 (MQ=255)

                                                                                    tgctgGCACGTTGCTCATCAATCAATCTCCAAAAAAGTAAATCACGCGACGGCTTTGCCGTTACGCACAAAAACAGGTTTTGTCACTTTAAcc     >  1:7837173/1‑93 (MQ=255)

                                                                                     gctgGCACGTTGCTCATCAATCAATCTCCAAAAAAGTAAATCACGCGACGGCTTTGCCGTTACGCACAAAAACAGGTTTTGTCACTTTAACCg    <  1:6910207/93‑1 (MQ=255)

                                                                                     gctgGCACGTTGCTCATCAATCAATCTCCAAAAAAGTAAATCACGCGACGGCTTTGCCGTTACGCACAAAAACAGGTTTTGTCACTTTAACCg    <  2:7366194/93‑1 (MQ=255)

                                                                                      ctgGCACGTTGCTCATCAATCAATCTCCAAAAAAGTAAATCACGCGACGGCTTTGCCGTTACGCACAAAAACAGGTTTTGTCACTTTAACCgg   <  1:5822557/93‑1 (MQ=255)

                                                                                       tgGCACGTTGCTCATCAATCAATCTCCAAAAAAGTAAATCACGCGACGGCTTTGCCGTTACGCACAAAAACAGGTTTTGTCACTTTAACCGGc  <  2:1723763/93‑1 (MQ=255)

CTGAGCATGTTCGGTAATACCAACGGTGTAAGTGCCGTCGGCTTCTTTACGCAGCCATTCGTGTTCTTTGCTGTATTTCAGTTCTGCTGGTACGTTGCTCATCAATCAATCTCCAAAAAAGTAAATCACGCGACGGCTTTGCCGTTACGCACAAAAACAGGTTTTGTCACTTTAACCGGC  >  NC_000913/3047468‑3047647

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 31 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`