BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,079,142	A→G	G171G (GGT→GGC)	tktA ←	transketolase 1 isozyme

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,079,142	0	A	G	99.7%	947.3 / ‑5.3	302	G171G (GGT→GGC)	tktA	transketolase 1 isozyme
Reads supporting (aligned to +/- strand): ref base A (1/0); new base G (166/135); total (167/135)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.83e-01

Only 100 of 314 total aligned reads displayed.

GGTGTCGTCGGTGAACCAGCCTTCAACGTGACCATCGATAGAAATACCGTTGTCATCGTAGAATGCAATCAGTTTACCCAGCTTCAGCGTACCCGCCAGAGAGCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTG  >  NC_000913/3079052‑3079231

ggTGTCGTCGGTGAACCAGCCTTCAACGTGACCATCGATAGAAATACCGTTGTCATCGTAGAATGCAATCAGTTTACCCAGCTTCAGCGTGcc                                                                                         <  2:7275728/93‑1 (MQ=255)

ggTGTCGTCGGTGAACCAGCCTTCAACGTGACCATCGATAGAAATACCGTTGTCATCGTAGAATGCAATCAGTTTACCCAGCTTCAGCGTGcc                                                                                         <  1:6093491/93‑1 (MQ=255)

ggTGTCGTCGGTGAACCAGCCTTCAACGTGACCATCGATAGAAATACCGTTGTCATCGTAGAATGCAATCAGTTTACCCAGCTTCAGCGTGcc                                                                                         <  1:7296494/93‑1 (MQ=255)

 gtgtCGTCGGTGAACCAGCCTTCAACGTGACCATCGATAGAAATACCGTTGTCATCGTAGAATGCAATCAGTTTACCCAGCTTCAGCGTGccc                                                                                        <  2:4779309/93‑1 (MQ=255)

 gtgtCGTCGGTGAACCAGCCTTCAACGTGACCATCGATAGAAATACCGTTGTCATCGTAGAATGCAATCAGTTTACCCAGCTTCAGCGTGccc                                                                                        <  2:2969440/93‑1 (MQ=255)

   gtcgtcGGTGAACCAGCCTTCAACGTGACCATCGATAGAAATACCGTTGTCATCGTAGAATGCAATCAGTTTACCCAGCTTCAGCGTGCCCGc                                                                                      >  2:4213943/1‑93 (MQ=255)

   gtcgtcGGTGAACCAGCCTTCAACGTGACCATCGATAGAAATACCGTTGTCATCGTAGAATGCAATCAGTTTACCCAGCTTCAGCGTGCCCGc                                                                                      <  1:4359414/93‑1 (MQ=255)

    tcgtcgGTGAACCAGCCTTCAACGTGACCATCGATAGAAATACCGTTGTCATCGTAGAATGCAATCAGTTTACCCAGCTTCAGCGTGCCCGcc                                                                                     >  2:1466086/1‑93 (MQ=255)

    tcgtcgGTGAACCAGCCTTCAACGTGACCATCGATAGAAATACCGTTGTCATCGTAGAATGCAATCAGTTTACCCAGCTTCAGCGTGCCCGcc                                                                                     >  2:3237898/1‑93 (MQ=255)

    tcgtcgGTGAACCAGCCTTCAACGTGACCATCGATAGAAATACCGTTGTCATCGTAGAATGCAATCAGTTTACCCAGCTTCAGCGTGCCCGcc                                                                                     >  2:5231594/1‑93 (MQ=255)

      gtcgGTGAACCAGCCTTCAACGTGACCATCGATAGAAATACCGTTGTCATCGTAGAATGCAATCAGTTTACCCAGCTTCAGCGTGCCCGCCag                                                                                   <  1:400776/93‑1 (MQ=255)

      gtcgGTGAACCAGCCTTCAACGTGACCATCGATAGAAATACCGTTGTCATCGTAGAATGCAATCAGTTTACCCAGCTTCAGCGTGCCCGCCag                                                                                   >  1:6565489/1‑93 (MQ=255)

      gtcgGTGAACCAGCCTTCAACGTGACCATCGATAGAAATACCGTTGTCATCGTAGAATGCAATCAGTTTACCCAGCTTCAGCGTGCCCGCCag                                                                                   >  2:1639236/1‑93 (MQ=255)

       tcgGTGAACCAGCCTTCAACGTGACCATCGATAGAAATACCGTTGTCATCGTAGAATGCAATCAGTTTACCCAGCTTCAGCGTGCCCGCCaga                                                                                  >  2:2625239/1‑93 (MQ=255)

       tcgGTGAACCAGCCTTCAACGTGACCATCGATAGAAATACCGTTGTCATCGTAGAATGCAATCAGTTTACCCAGCTTCAGCGTGCCCGCCaga                                                                                  <  1:4816268/93‑1 (MQ=255)

       tcgGTGAACCAGCCTTCAACGTGACCATCGATAGAAATACCGTTGTCATCGTAGAATGCAATCAGTTTACCCAGCTTCAGCGTGCCCGCCaga                                                                                  <  2:2155857/93‑1 (MQ=255)

       tcgGTGAACCAGCCTTCAACGTGACCATCGATAGAAATACCGTTGTCATCGTAGAATGCAATCAGTTTACCCAGCTTCAGCGTGCCCGCCaga                                                                                  <  2:373034/93‑1 (MQ=255)

        cgGTGAACCAGCCTTCAACGTGACCATCGATAGAAATACCGTTGTCATCGTAGAATGCAATCAGTTTACCCAGCTTCAGCGTGCCCGCCagag                                                                                 >  1:1648558/1‑93 (MQ=255)

         ggTGAACCAGCCTTCAACGTGACCATCGATAGAAATACCGTTGTCATCGTAGAATGCAATCAGTTTACCCAGCTTCAGCGTGCCCGCCagaga                                                                                >  1:283401/1‑93 (MQ=255)

          gTGAACCAGCCTTCAACGTGACCATCGATAGAAATACCGTTGTCATCGTAGAATGCAATCAGTTTACCCAGCTTCAGCGTGCCCGCCagagag                                                                               >  2:5328324/1‑93 (MQ=255)

           tGAACCAGCCTTCAACGTGACCATCGATAGAAATACCGTTGTCATCGTAGAATGCAATCAGTTTACCCAGCTTCAGCGTGCCCGCCAGAGAGc                                                                              >  1:5251761/1‑93 (MQ=255)

            gAACCAGCCTTCAACGTGACCATCGATAGAAATACCGTTGTCATCGTAGAATGCAATCAGTTTACCCAGCTTCAGCGTGCCCGCCAGAGAGCa                                                                             <  2:178027/93‑1 (MQ=255)

              aCCAGCCTTCAACGTGACCATCGATAGAAATACCGTTGTCATCGTAGAATGCAATCAGTTTACCCAGCTTCAGCGTGCCCGCCAGAGAGCaaa                                                                           <  1:6890781/93‑1 (MQ=255)

              aCCAGCCTTCAACGTGACCATCGATAGAAATACCGTTGTCATCGTAGAATGCAATCAGTTTACCCAGCTTCAGCGTGCCCGCCAGAGAGCaaa                                                                           >  2:5792233/1‑93 (MQ=255)

                cAGCCTTCAACGTGACCATCGATAGAAATACCGTTGTCATCGTAGAATGCAATCAGTTTACCCAGCTTCAGCGTGCCCGCCAGAGAGCAAACt                                                                         >  2:5193675/1‑93 (MQ=255)

                  gCCTTCAACGTGACCATCGATAGAAATACCGTTGTCATCGTAGAATGCAATCAGTTTACCCAGCTTCAGCGTGCCCGCCAGAGAGCAAACTTc                                                                       <  2:6404886/93‑1 (MQ=255)

                  gCCTTCAACGTGACCATCGATAGAAATACCGTTGTCATCGTAGAATGCAATCAGTTTACCCAGCTTCAGCGTGCCCGCCAGAGAGCAAACTTc                                                                       <  1:6580521/93‑1 (MQ=255)

                    cTTCAACGTGACCATCGATAGAAATACCGTTGTCATCGTAGAATGCAATCAGTTTACCCAGCTTCAGCGTGCCCGCCAGAGAGCAAACTTCGt                                                                     >  2:5699677/1‑93 (MQ=255)

                     ttCAACGTGACCATCGATAGAAATACCGTTGTCATCGTAGAATGCAATCAGTTTACCCAGCTTCAGCGTGCCCGCCAGAGAGCAAACTTCGTg                                                                    >  2:6640262/1‑93 (MQ=255)

                     ttCAACGTGACCATCGATAGAAATACCGTTGTCATCGTAGAATGCAATCAGTTTACCCAGCTTCAGCGTGCCCGCCAGAGAGCAAACTTCGTg                                                                    <  1:1400980/93‑1 (MQ=255)

                      tCAACGTGACCATCGATAGAAATACCGTTGTCATCGTAGAATGCAATCAGTTTACCCAGCTTCAGCGTGCCCGCCAGAGAGCAAACTTCGTgg                                                                   <  2:4903859/93‑1 (MQ=255)

                        aaCGTGACCATCGATAGAAATACCGTTGTCATCGTAGAATGCAATCAGTTTACCCAGCTTCAGCGTGCCCGCCAGAGCGCAACCTTAGTGGga                                                                 >  1:6145551/1‑93 (MQ=255)

                        aaCGTGACCATCGATAGAAATACCGTTGTCATCGTAGAATGCAATCAGTTTACCCAGCTTCAGCGTGCCCGCCAGAGAGCAAACTTCGTGGga                                                                 <  1:6368520/93‑1 (MQ=255)

                        aaCGTGACCATCGATAGAAATACCGTTGTCATCGTAGAATGCAATCAGTTTACCCAGCTTCAGCGTGCCCGCCAGAGAGCAAACTTCGTGGga                                                                 >  2:7211246/1‑93 (MQ=255)

                          cGTGACCATCGATAGAAATACCGTTGTCATCGTAGAATGCAATCAGTTTACCCAGCTTCAGCGTGCCCGCCAGAGAGCAAACTTCGTGGgaga                                                               <  1:1179203/93‑1 (MQ=255)

                          cGTGACCATCGATAGAAATACCGTTGTCATCGTAGAATGCAATCAGTTTACCCAGCTTCAGCGTGCCCGCCAGAGAGCAAACTTCGTGGgaga                                                               <  2:1057377/93‑1 (MQ=255)

                           gTGACCATCGATAGAAATACCGTTGTCATCGTAGAATGCAATCAGTTTACCCAGCTTCAGTGTGCCCGCCAGAGAGCAAACTTCGTGGGAGAt                                                              <  2:54517/93‑1 (MQ=255)

                              aCCATCGATAGAAATACCGTTGTCATCGTAGAATGCAATCAGTTTACCCAGCTTCAGCGTGCCCGCCAGAGAGCAAACTTCGTGGGAGATGcc                                                           >  2:3508218/1‑93 (MQ=255)

                               ccATCGATAGAAATACCGTTGTCATCGTAGAATGCAATCAGTTTACCCAGCTTCAGCGTGCCCGCCAGAGAGCAAACTTCGTGGGAGATGCCt                                                          >  2:1932160/1‑93 (MQ=255)

                               ccATCGATAGAAATACCGTTGTCATCGTAGAATGCAATCAGTTTACCCAGCTTCAGCGTGCCCGCCAGAGAGCAAACTTCGTGGGAGATGCCt                                                          >  2:7143504/1‑93 (MQ=255)

                                 aTCGATAGAAATACCGTTGTCATCGTAGAATGCAATCAGTTTACCCAGCTTCAGCGTGCCCGCCAGAGAGCAAACTTCGTGGGAGATGCCTTc                                                        >  1:7347580/1‑93 (MQ=255)

                                 aTCGATAGAAATACCGTTGTCATCGTAGAATGCAATCAGTTTACCCAGCTTCAGCGTGCCCGCCAGAGAGCAAACTTCGTGGGAGATGCCTTc                                                        >  2:4472235/1‑93 (MQ=255)

                                 aTCGATAGAAATACCGTTGTCATCGTAGAATGCAATCAGTTTACCCAGCTTCAGCGTGCCCGCCAGAGAGCAAACTTCGTGGGAGATGCCTTc                                                        >  2:6083598/1‑93 (MQ=255)

                                 aTCGATAGAAATACCGTTGTCATCGTAGAATGCAATCAGTTTACCCAGCTTCAGCGTGCCCGCCAGAGAGCAAACTTCGTGGGAGATGCCTTc                                                        <  2:7861621/93‑1 (MQ=255)

                                 aTCGATAGAAATACCGTTGTCATCGTAGAATGCAATCAGTTTACCCAGCTTCAGCGTGCCCGCCAGAGAGCAAACTTCGTGGGAGATGCCTTc                                                        >  1:2988763/1‑93 (MQ=255)

                                   cGATAGAAATACCGTTGTCATCGTAGAATGCAATCAGTTTACCCAGCTTCAGCGTGCCCGCCAGAGAGCAAACTTCGTGGGAGATGCCTTCca                                                      <  1:1559499/93‑1 (MQ=255)

                                    gATAGAAATACCGTTGTCATCGTAGAATGCAATCAGTTTACCCAGCTTCAGCGTGCCCGCCAGAGAGCAAACTTCGTGGGAGATGCCTTCcat                                                     >  1:7608585/1‑93 (MQ=255)

                                     aTAGAAATACCGTTGTCATCGTAGAATGCAATCAGTTTACCCAGCTTCAGCGTGCCCGCCAGAGAGCAAACTTCGTGGGAGATGCCTTCcatc                                                    <  2:431227/93‑1 (MQ=255)

                                     aTAGAAATACCGTTGTCATCGTAGAATGCAATCAGTTTACCCAGCTTCAGCGTGCCCGCCAGAGAGCAAACTTCGTGGGAGATGCCTTCcatc                                                    <  1:5390955/93‑1 (MQ=255)

                                      tAGAAATACCGTTGTCATCGTAGAATGCAATCAGTTTACCCAGCTTCAGCGTGCCCGCCAGAGAGCAAACTTCGTGGGAGATGCCTTCcatca                                                   >  1:7172799/1‑93 (MQ=255)

                                        gAAATACCGTTGTCATCGTAGAATGCAATCAGTTTACCCAGCTTCAGCGTGCCCGCCAGAGAGCAAACTTCGTGGGAGATGCCTTCCATCATg                                                 >  2:4657143/1‑93 (MQ=255)

                                          aaTACCGTTGTCATCGTAGAATGCAATCAGTTTACCCAGCTTCAGCGTGCCCGCCAGAGAGCAAACTTCGTGGGAGATGCCTTCCATCATGCa                                               <  1:5626744/93‑1 (MQ=255)

                                            tACCGTTGTCATCGTAGAATGCAATCAGTTTACCCAGCTTCAGCGTGCCCGCCAGAGAGCAAACTTCGTGGGAGATGCCTTCCATCATGCAGc                                             >  1:5734512/1‑93 (MQ=255)

                                              ccGTTGTCATCGTAGAATGCAATCAGTTTACCCAGCTTCAGCGTGCCCGCCAGAGAGCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCg                                           <  2:6225896/93‑1 (MQ=255)

                                                 ttGTCATCGTAGAATGCAATCAGTTTACCCAGCTTCAGCGTGCCCGCCAGAGAGCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCg                                        >  2:7480345/1‑93 (MQ=255)

                                                 ttGTCATCGTAGAATGCAATCAGTTTACCCAGCTTCAGCGTGCCCGCCAGAGAGCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCg                                        >  2:4541129/1‑93 (MQ=255)

                                                  tGTCATCGTAGAATGCAATCAGTTTACCCAGCTTCAGCGTGCCCGCCAGAGAGCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGc                                       <  1:2689300/93‑1 (MQ=255)

                                                   gTCATCGTAGAATGCAATCAGTTTACCCAGCTTCAGCGTGCCCGCCAGAGAGCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGcc                                      >  1:1315566/1‑93 (MQ=255)

                                                     cATCGTAGAATGCAATCAGTTTACCCAGCTTCAGCGTGCCCGCCAGAGAGCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCa                                    <  1:786149/93‑1 (MQ=255)

                                                      aTCGTAGAATGCAATCAGTTTACCCAGCTTCAGCGTGCCCGCCAGAGAGCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCAt                                   <  1:3388252/93‑1 (MQ=255)

                                                      aTCGTAGAATGCAATCAGTTTACCCAGCTTCAGCGTGCCCGCCAGAGAGCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCAt                                   >  1:4652178/1‑93 (MQ=255)

                                                       tCGTAGAATGCAATCAGTTTACCCAGCTTCAGCGTGCCCGCCAGAGAGCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATg                                  >  1:317342/1‑93 (MQ=255)

                                                        cGTAGAATGCAATCAGTTTACCCAGCTTCAGCGTGCCCGCCAGAGAGCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGa                                 <  1:5128787/93‑1 (MQ=255)

                                                         gTAGAATGCAATCAGTTTACCCAGCTTCAGCGTGCCCGCCAGAGAGCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGaa                                >  1:4943349/1‑93 (MQ=255)

                                                          tAGAATGCAATCAGTTTACCCAGCTTCAGCGTGCCCGCCAGAGAGCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAg                               >  1:3574640/1‑93 (MQ=255)

                                                            gAATGCAATCAGTTTACCCAGCTTCAGCGTGCCCGCCAGAGAGCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGc                             >  2:2480348/1‑93 (MQ=255)

                                                            gAATGCAATCAGTTTACCCAGCTTCAGCGTGCCCGCCAGAGAGCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGc                             >  1:1428451/1‑93 (MQ=255)

                                                            gAATGCAATCAGTTTACCCAGCTTCAGCGTGCCCGCCAGAGAGCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGc                             <  1:3765417/93‑1 (MQ=255)

                                                              aTGCAATCAGTTTACCCAGCTTCAGCGTGCCCGCCAGAGAGCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGt                           >  1:821935/1‑93 (MQ=255)

                                                               tGCAATCAGTTTACCCAGCTTCAGCGTGCCCGCCAGAGAGCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTa                          <  1:4693432/93‑1 (MQ=255)

                                                               agCAATCAGTTTACCCAGCTTCAGCGTGCCCGCCAGAGAGCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTa                          <  2:1514518/92‑1 (MQ=255)

                                                                gCAATCAGTTTACCCAGCTTCAGCGTGCCCGCCAGAGAGCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAg                         <  1:2372244/93‑1 (MQ=255)

                                                                gCAATCAGTTTACCCAGCTTCAGCGTGCCCGCCAGAGAGCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAg                         <  1:6781812/93‑1 (MQ=255)

                                                                gCAATCAGTTTACCCAGCTTCAGCGTGCCCGCCAGAGAGCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAg                         >  1:6201899/1‑93 (MQ=255)

                                                                  aaTCAGTTTACCCAGCTTCAGCGTGCCCGCCAGAGAGCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGgt                       <  1:7523942/93‑1 (MQ=255)

                                                                   aTCAGTTTACCCAGCTTCAGCGTGCCCGCCAGAGAGCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGgtg                      >  2:501042/1‑93 (MQ=255)

                                                                   aTCAGTTTACCCAGCTTCAGCGTGCCCGCCAGAGAGCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGgtg                      >  1:6455965/1‑93 (MQ=255)

                                                                    tCAGTTTACCCAGCTTCAGCGTGCCCGCCAGAGAGCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGgtgt                     <  1:4202810/93‑1 (MQ=255)

                                                                      aGTTTACCCAGCTTCAGCGTGCCCGCCAGAGAGCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAg                   <  2:6719830/93‑1 (MQ=255)

                                                                       gTTTACCCAGCTTCAGCGTGCCCGCCAGAGAGCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGt                  <  1:6807151/93‑1 (MQ=255)

                                                                       gTTTACCCAGCTTCAGCGTGCCCGCCAGAGAGCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGt                  >  2:3053759/1‑93 (MQ=255)

                                                                        tttACCCAGCTTCAGCGTGCCCGCCAGAGAGCATACTTCGTGGGAGATGGCTTCCATCATGCAGCCGTAGTCCTTGAATGCGTCGGCGTAGTg                 >  2:7665253/1‑93 (MQ=255)

                                                                         ttACCCAGCTTCAGCGTGCCCGCCAGAGAGCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTgg                <  1:964576/93‑1 (MQ=255)

                                                                          tACCCAGCTTCAGCGTGCCCGCCAGAGAGCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGt               <  1:3334745/93‑1 (MQ=255)

                                                                            cccAGCTTCAGCGTGCCCGCCAGAGAGCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCg             >  2:6912852/1‑93 (MQ=255)

                                                                                gCTTCAGCGTGCCCGCCAGAGAGCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACaa         <  1:1751279/93‑1 (MQ=255)

                                                                                gCTTCAGCGTGCCCGCCAGAGAGCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACaa         <  2:4409882/93‑1 (MQ=255)

                                                                                gCTTCAGCGTGCCCGCCAGAGAGCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACaa         >  1:2605268/1‑93 (MQ=255)

                                                                                gCTTCAGCGTGCCCGCCAGAGAGCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACaa         <  1:3204030/93‑1 (MQ=255)

                                                                                 cTTCAGCGTGCCCGCCAGAGAGCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAAt        <  2:5252856/93‑1 (MQ=255)

                                                                                 cTTCAGCGGGCCCGCCAGAGAGCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAAt        <  1:2024081/93‑1 (MQ=255)

                                                                                  ttCAGCGTGCCCGCCAGAGAGCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATg       >  1:1936490/1‑93 (MQ=255)

                                                                                  ttCAGCGTGCCCGCCAGAGAGCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATg       >  1:1063510/1‑93 (MQ=255)

                                                                                   tCAGCGTGCCCGCCAGAGAGCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGt      <  2:5930932/93‑1 (MQ=255)

                                                                                   tCAGCGTGCCCGCCAGAGAGCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGt      >  1:1487131/1‑93 (MQ=255)

                                                                                   tCAGCGTGCCCGCCAGAGAGCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGt      <  2:4054115/93‑1 (MQ=255)

                                                                                   tCAGCGTGCCCGCCAGAGAGCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGt      >  2:1260468/1‑93 (MQ=255)

                                                                                   tCAGCGTGCCCGCCAGAGAGCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGt      >  2:7614463/1‑93 (MQ=255)

                                                                                    cAGCGTGCCCGCCAGAGAGCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTc     >  1:2765335/1‑93 (MQ=255)

                                                                                       cGTGCCCGCCAGAGAGCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTg  >  1:594164/1‑93 (MQ=255)

GGTGTCGTCGGTGAACCAGCCTTCAACGTGACCATCGATAGAAATACCGTTGTCATCGTAGAATGCAATCAGTTTACCCAGCTTCAGCGTACCCGCCAGAGAGCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTG  >  NC_000913/3079052‑3079231

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 23 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`