BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,157,790	G→A	C457C (TGC→TGT)	ygiRQ ←	Fe‑S oxidoreductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,157,790	0	G	A	99.4%	1101.2 / ‑6.5	313	C457C (TGC→TGT)	ygiRQ	Fe‑S oxidoreductase
Reads supporting (aligned to +/- strand): ref base G (0/2); new base A (140/171); total (140/173)
Fisher's exact test for biased strand distribution p-value = 5.04e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 333 total aligned reads displayed.

TTTAATGCCTTTCAGATCACGCGCACGGCGATAGAGGTTGATCGTTGGTTCGTGGTTCGTGTCCATGTGCGGACAAATATCCGGATAAACGCACGACAAACGGCGACAAGTTTGTTCAGCGCGTGGCGATTTGCAGCGCAACATATACATGTTGGCAGTTGGCCCACCAAGATCGGAAAT  >  NC_000913/3157700‑3157879

tttAATGCCTTTCAGATCACGCGCACGGCGATAGAGGTTGATCGTTGGTTCGTGGTTCGTGTCCATGTGCGGACAAATATCCGGATAAAcaca                                                                                         <  2:4897663/93‑1 (MQ=255)

tttAATGCCTTTCAGATCACGCGCACGGCGATAGAGGTTGATCGTTGGTTCGTGGTTCGTGTCCATGTGCGGACAAATATCCGGATAAAcaca                                                                                         <  1:4077010/93‑1 (MQ=255)

 ttAATGCCTTTCAGATCACGCGCACGGCGATAGAGGTTGATCGTTGGTTCGTGGTTCGTGTCCATGTGCGGACAAATATCCGGATAAacacac                                                                                        >  2:1055812/1‑93 (MQ=255)

  tAATGCCTTTCAGATCACGCGCACGGCGATAGAGGTTGATCGTTGGTTCGTGGTTCGTGTCCATGTGCGGACAAATATCCGGATAAACACACg                                                                                       >  2:4999976/1‑93 (MQ=255)

  tAATGCCTTTCAGATCACGCGCACGGCGATAGAGGTTGATCGTTGGTTCGTGGTTCGTGTCCATGTGCGGACAAATATCCGGATAAACACACg                                                                                       >  2:2991668/1‑93 (MQ=255)

   aaTGCCTTTCAGATCACGCGCACGGCGATAGAGGTTGATCGTTGGTTCGTGGTTCGTGTCCATGTGCGGACAAATATCCGGATAAACACACGa                                                                                      <  1:2160361/93‑1 (MQ=255)

     tGCCTTTCAGATCACGCGCACGGCGATAGAGGTTGATCGTTGGTTCGTGGTTCGTGTCCATGTGCGGACAGATATCCGGATAAACACACGACa                                                                                    >  1:6659607/1‑93 (MQ=255)

     tGCCTTTCAGATCACGCGCACGGCGATAGAGGTTGATCGTTGGTTCGTGGTTCGTGTCCATGTGCGGACAAATATCCGGATAAACACACGACa                                                                                    >  1:7927253/1‑93 (MQ=255)

       ccTTTCAGATCACGCGCACGGCGATAGAGGTTGATCGTTGGTTCGTGGTTCGTGTCCATGTGCGGACAAATATCCGGATAAACACACGACaaa                                                                                  <  2:7483758/93‑1 (MQ=255)

       ccTTTCAGATCACGCGCACGGCGATAGAGGTTGATCGTTGGTTCGTGGTTCGTGTCCATGTGCGGACAAATATCCGGATAAACACACGACaaa                                                                                  <  2:5112048/93‑1 (MQ=255)

        cTTTCAGATCACGCGCACGGCGATAGAGGTTGATCGTTGGTTCGTGGTTCGTGTCCATGTGCGGACAAATATCCGGATAAACACACGACAAAc                                                                                 <  1:6211605/93‑1 (MQ=255)

         tttCAGATCACGCGCACGGCGATAGAGGTTGATCGTTGGTTCGTGGTTCGTGTCCATGTGCGGACAAATATCCGGATAAACACACGACAAACg                                                                                >  2:4173847/1‑93 (MQ=255)

         tttCAGATCACGCGCACGGCGATAGAGGTTGATCGTTGGTTCGTGGTTCGTGTCCATGTGCGGACAAATATCCGGATAAACACACGACAAACg                                                                                <  1:3234106/93‑1 (MQ=255)

         tttCAGATCACGCGCACGGCGATAGAGGTTGATCGTTGGTTCGTGGTTCGTGTCCATGTGCGGACAAATATCCGGATAAACACACGACAAACg                                                                                <  1:5169321/93‑1 (MQ=255)

           tCAGATCACGCGCACGGCGATAGAGGTTGATCGTTGGTTCGTGGTTCGTGTCCATGTGCGGACAAATATCCGGATAAACACACGACAAACGGc                                                                              >  1:5568435/1‑93 (MQ=255)

           tCAGATCACGCGCACGGCGATAGAGGTTGATCGTTGGTTCGTGGTTCGTGTCCATGTGCGGACAAATATCCGGATAAACACACGACAAACGGc                                                                              <  1:6609352/93‑1 (MQ=255)

             aGATCACGCGCACGGCGATAGAGGTTGATCGTTGGTTCGTGGTTCGTGTCCATGTGCGGACAAATATCCGGATAAACACACGACAAACGGCGa                                                                            >  1:4875004/1‑93 (MQ=255)

             aGATCACGCGCACGGCGATAGAGGTTGATCGTTGGTTCGTGGTTCGTGTCCATGTGCGGACAAATATACGGATAAACACACGACAAACGGCGa                                                                            >  2:568620/1‑93 (MQ=255)

                tCACGCGCACTGCGATAGAGGTTGATCGTTGGTTCGTGGTTCGTGTCCATGTGCGGACAAATATCCGGATAAACACACGACAAACGGCGACaa                                                                         <  2:2220561/93‑1 (MQ=255)

                 cACGCGCACGGCGATAGAGGTTGATCGTTGGTTCGTGGTTCGTGTCCATGTGCGGACAAATATCCGGATAAACACACGACAAACGGCGACAAg                                                                        <  1:5879896/93‑1 (MQ=255)

                       cACGGCGATAGAGGTTGATCGTTGGTTCGTGGTTCGTGTCCATGTGCGGACAAATATCCGGATAAACACACGACAAACGGCGACAAGTTTGtt                                                                  <  2:1734562/93‑1 (MQ=255)

                        aCGGCGATAGAGGTTGATCGTTGGTTCGTGGTTCGTGTCCATGTGCGGACAAATATCCGGATAAACACACGACAAACGGCGACAAGTTTGTTc                                                                 >  2:5606481/1‑93 (MQ=255)

                         cGGCGATAGAGGTTGATCGTTGGTTCGTGGTTCGTGTCCATGTGCGGACAAATATCCGGATAAACACACGACAAACGGCGACAAGTTTGTTCa                                                                <  2:873302/93‑1 (MQ=255)

                          ggCGATAGAGGTTGATCGTTGGTTCGTGGTTCGTGTCCATGTGCGGACAAATATCCGGATAAACACACGACAAACGGCGACAAGTTTGTTCAg                                                               <  1:7178095/93‑1 (MQ=255)

                          ggCGATAGAGGTTGATCGTTGGTTCGTGGTTCGTGTCCATGTGCGGACAAATATCCGGATAAACACACGACAAACGGCGACAAGTTTGTTCAg                                                               >  1:169032/1‑93 (MQ=255)

                           gCGATAGAGGTTGATCGTTGGTTCGTGGTTCGTGTCCATGTGCGGACAAATATCCGGATAAACACACGACAAACGGCGACAAGTTTGTTCAgc                                                              <  1:3935152/93‑1 (MQ=255)

                           gCGATAGAGGTTGATCGTTGGTTCGTGGTTCGTGTCCATGTGCGGACAAATATCCGGATAAACACACGACAAACGGCGACAAGTTTGTTCAgc                                                              <  1:2327930/93‑1 (MQ=255)

                              aTAGAGGTTGATCGTTGGTTCGTGGTTCGTGTCCATGTGCGGACAAATATCCGGATAAACACACGACAAACGGCGACAAGTTTGTTCAGcgcg                                                           >  1:4952392/1‑93 (MQ=255)

                              aTAGAGGTTGATCGTTGGTTCGTGGTTCGTGTCCATGTGCGGACAAATATCCGGATAAACACACGACAAACGGCGACAAGTTTGTTCAGcgcg                                                           >  2:4444325/1‑93 (MQ=255)

                               tAGAGGTTGATCGTTGGTTCGTGGTTCGTGTCCATGTGCGGACAAATATCCGGATAAACACACGACAAACGGCGACAAGTTTGTTCAGCGCGt                                                          >  1:4243069/1‑93 (MQ=255)

                               tAGAGGTTGATCGTTGGTTCGTGGTTCGTGTCCATGTGCGGACAAATATCCGGATAAACACACGACAAACGGCGACAAGTTTGTTCAGCGCGt                                                          <  2:2067714/93‑1 (MQ=255)

                               tAGAGGTTGATCGTTGGTTCGTGGTTCGTGTCCATGTGCGGACAAATATCCGGATAAACACACGACAAACGGCGACAAGTTTGTTCAGCGCGt                                                          <  1:2835460/93‑1 (MQ=255)

                               tAGAGGTTGATCGTTGGTTCGTGGTTCGTGTCCATGTGCGGACAAATATCCGGATAAACACACGACAAACGGCGACAAGTTTGTTCAGCGCGt                                                          <  2:7237758/93‑1 (MQ=255)

                                 gagGTTGATCGTTGGTTCGTGGTTCGTGTCCATGTGCGGACAAATATCCGGATAAACACACGACAAACGGCGACAAGTTTGTTCAGCGCGTgg                                                        >  1:6271445/1‑93 (MQ=255)

                                 gagGTTGATCGTTGGTTCGTGGTTCGTGTCCATGTGCGGACAAATATCCGGATAAACACACGACAAACGGCGACAAGTTTGTTCAGCGCGTgg                                                        >  2:6016745/1‑93 (MQ=255)

                                  agtttGATCGTTGGTTCGTGGTTCGTGTCCATGTGCGGACAAATATCCGGATAAACACACGACAAACGGCGACAAGTTTGTTCAGCGCGTGGc                                                       <  2:3373468/90‑1 (MQ=255)

                                  agGTTGATCGTTGGTTCGTGGTTCGTGTCCATGTGCGGACAAATATCCGGATAAACACACGACAAACTGCGACAAGTTTGTTCAGCGCGGGGc                                                       >  1:2536427/1‑93 (MQ=255)

                                  agGTTGATCGTTGGTTCGTGGTTCGTGTCCATGTGCGGACAAATATCCGGATAAACACACGACAAACGGCGACAAGTTTGTTCAGCGCGTGGc                                                       <  2:7408935/93‑1 (MQ=255)

                                  agGTTGATCGTTGGTTCGTGGTTCGTGTCCATGTGCGGACAAATATCCGGATAAACACACGACAAACGGCGACAAGTTTGTTCAGCGCGTGGc                                                       <  2:1465921/93‑1 (MQ=255)

                                     ttGATCGTTGGTTCGTGGTTCGTGTCCATGTGCGGACAAATATCCGGATAAACACACGACAAACGGCGACAAGTTTGTTCAGCGCGTGGCGAt                                                    <  1:7441600/93‑1 (MQ=255)

                                       gATCGTTGGTTCGTGGTTCGTGTCCATGTGCGGACAAATATCCGGATAAACACACGACAAACGGCGACAAGTTTGTTCAGCGCGTGGCGAttt                                                  >  1:1769620/1‑93 (MQ=255)

                                       gATCGTTGGTTCGTGGTTCGTGTCCATGTGCGGACAAATATCCGGATAAACACACGACAAACGGCGACAAGTTTGTTCAGCGCGTGGCGAttt                                                  >  2:1189125/1‑93 (MQ=255)

                                       gATCGTTGGTTCGTGGTTCGTGTCCATGTGCGGACAAATATCCGGATAAACACACGACAAACGGCGACAAGTTTGTTCAGCGCGTGGCGAttt                                                  >  2:6617697/1‑93 (MQ=255)

                                          cGTTGGTTCGTGGTTCGTGTCCACGTGCGGACAAATATCCGGATAAACACACGACAAACGGCGACAAGTTTGTTCAGCGCGTGGCGATTTGCa                                               <  2:4277833/93‑1 (MQ=255)

                                           gTTGGTTCGTGGTTCGTGTCCATGTGCGGACAAATATCCGGATAAACACACGACAAACGGCGACAAGTTTGTTCAGCGCGTGGCGATTTGCAg                                              <  2:2741063/93‑1 (MQ=255)

                                            ttGGTTCGTGGTTCGTGTCCATGTGCGGACAAATATCCGGATAAACACACGACAAACGGCGACAAGTTTGTTCAGCGCGTGGCGATTTGCAgc                                             <  2:3778741/93‑1 (MQ=255)

                                              ggttcgtggttcgtGTCCATGTGCGGACAAATATCCGGATAAACACACGACAAACGGCGACAAGTTTGTTCAGCGCGTGGCGATTTGCAgcgc                                           >  1:199032/1‑93 (MQ=255)

                                              ggttcgtggttcgtGTCCATGTGCGGACAAATATCCGGATAAACACACGACAAACGGCGACAAGTTTGTTCAGCGCGTGGCGATTTGCAgcgc                                           >  2:5033955/1‑93 (MQ=255)

                                              ggttcgtggttcgtGTCCATGTGCGGACAAATATCCGGATAAACACACGACAAACGGCGACAAGTTTGTTCAGCGCGTGGCGATTTGCAgcgc                                           <  2:1493096/93‑1 (MQ=255)

                                                ttcgtggttcgtgTCCATGTGCGGACAAATATCCGGATAAACACACGACAAACGGCGACAAGTTTGTTCAGCGCGTGGCGATTTGCAGCGCaa                                         >  2:6527649/1‑93 (MQ=255)

                                                ttcgtggttcgtgTCCATGTGCGGACAAATATCCGGATAAACACACGACAAACGGCGACAAGTTTGTTCAGCGCGTGGCGATTTGCAGCGCaa                                         >  1:7596578/1‑93 (MQ=255)

                                                  cgtggttcgtgTCCATGTGCGGACAAATATCCGGATAAACACACGACAAACGGCGACAAGTTTGTTCAGCGCGTGGCGATTTGCAGCGCAACa                                       <  2:1966610/93‑1 (MQ=255)

                                                   gtggttcgtgTCCATGTGCGGACAAATATCCGGATAAACACACGACAAACGGCGACAAGTTTGTTCAGCGCGTGGCGATTTGCAGCGCAACat                                      <  2:4642686/93‑1 (MQ=255)

                                                    tggttcgtgTCCATGTGCGGACAAATATCCGGATAAACACACGACAAACGGCGACAAGTTTGTTCAGCGCGTGGCGATTTGCAGCGCAACata                                     <  2:2271784/93‑1 (MQ=255)

                                                       ttcgtgTCCATGTGCGGACAAATATCCGGATAAACACACGACAAACGGCGACAAGTTTGTTCAGCGCGTGGCGATTTGCAGCGCAACATATAc                                  >  2:6227316/1‑93 (MQ=255)

                                                       ttcgtgTCCATGTGCGGACAAATATCCGGATAAACACACGACAAACGGCGACAAGTTTGTTCAGCGCGTGGCGATTTGCAGCGCAACATATAc                                  >  1:4634110/1‑93 (MQ=255)

                                                       ttcgtgTCCATGTGCGGACAAATATCCGGATAAACACACGACAAACGGCGACAAGTTTGTTCAGCGCGTGGCGATTTGCAGCGCAACATATAc                                  <  1:4356985/93‑1 (MQ=255)

                                                       ttcgtgTCCATGTGCGGACAAATATCCGGATAAACACACGACAAACGGCGACAAGTTTGTTCAGCGCGTGGCGATTTGCAGCGCAACATATAc                                  <  1:5673034/93‑1 (MQ=255)

                                                       ttcgtgTCCATGTGCGGACAAATATCCGGATAAACACACGACAAACGGCGACAAGTTTGTTCAGCGCGTGGCGATTTGCAGCGCAACATATAc                                  <  1:772539/93‑1 (MQ=255)

                                                        tcgtgTCCATGTGCGGACAAATATCCGGATAAACGCACGACAAACGGCGACAAGTTTGTTCAGCGCGTGGCGATTTGCAGCGCAACATATACa                                 <  1:5031931/93‑1 (MQ=255)

                                                         cgtgTCCATGTGCGGACAAATATCCGGATAAACACACGACAAACGGCGACAAGTTTGTTCAGCGCGTGGCGATTTGCAGCGCAACATATACAt                                >  2:1641085/1‑93 (MQ=255)

                                                           tgtCCATGTGCGGACAAATATCCGGATAAACACACGACAAACGGCGACAAGTTTGTTCAGCGCGTGGCGATTTGCAGCGCAACATATACATGt                              <  1:1103141/93‑1 (MQ=255)

                                                           tgtCCATGTGCGGACAAATATCCGGATAAACACACGACAAACGGCGACAAGTTTGTTCAGCGCGTGGCGATTTGCAGCGCAACATATACATGt                              <  1:7396453/93‑1 (MQ=255)

                                                            gtCCATGTGCGGCCACATATCCGGATAAACACACGACAAACGGCGACAAGTTTGTTCAGCGCGTGGCGATTTGCAGCGCAACATATACATGtt                             <  2:7025661/93‑1 (MQ=255)

                                                            gtCCATGTGCGGACAAATATCCGGATAAACACACGACAAACGGCGACAAGTTTGTTCAGCGCGTGGCGATTTGCAGCGCAACATATACATGtt                             <  1:7282104/93‑1 (MQ=255)

                                                            gtCCATGTGCGGACAAATATCCGGATAAACACACGACAAACGGCGACAAGTTTGTTCAGCGCGTGGCGATTTGCAGCGCAACATATACATGtt                             >  2:2342114/1‑93 (MQ=255)

                                                            gtCCATGTGCGGACAAATATCCGGATAAACACACGACAAACGGCGACAAGTTTGTTCAGCGCGTGGCGATTTGCAGCGCAACATATACATGtt                             <  2:4728830/93‑1 (MQ=255)

                                                             tCCATGTGCGGACAAATATCCGGATAAACACACGACAAACGGCGACAAGTTTGTTCAGCGCGTGGCGATTTGCAGCGCAACATATACATGTTg                            >  2:2460260/1‑93 (MQ=255)

                                                              ccATGTGCGGACAAATATCCGGATAAACACACGACAAACGGCGACAAGTTTGTTCAGCGCGTGGCGATTTGCAGCGCAACATATACATGTTgg                           <  1:875848/93‑1 (MQ=255)

                                                              ccATGTGCGGACAAATATCCGGATAAACACACGACAAACGGCGACAAGTTTGTTCAGCGCGTGGCGATTTGCAGCGCAACATATACATGTTgg                           <  1:4212976/93‑1 (MQ=255)

                                                               cATGTGCGGACAAATATCCGGATAAACACACGACAAACGGCGACAAGTTTGTTCAGCGCGTGGCGATTTGCAGCGCAACATATACATGTTGGc                          >  2:3945402/1‑93 (MQ=255)

                                                               cATGTGCGGACAAATATCCGGATAAACACACGACAAACGGCGACAAGTTTGTTCAGCGCGCGGCGATTTGCAGCGAAACATATACATGTTGGc                          >  1:7850003/1‑93 (MQ=255)

                                                                aTGTGCGGACAAATATCCGGATAAACACACGACAAACGGCGACAAGTTTGTTCAGCGCGTGGCGATTTGCAGCGCAACATATACATGTTGGCa                         >  2:360303/1‑93 (MQ=255)

                                                                 tgtgCGGACAAATATCCGGATAAACACACGACAAACGGCGACAAGTTTGTTCAGCGCGTGGCGATTTGCAGCGCAACATATACATGTTGGCAg                        >  1:2340274/1‑93 (MQ=255)

                                                                  gtgCGGACAAATATCCGGATAAACACACGACAAACGGCGACAAGTTTGTTCAGCGCGTGGCGATTTGCAGCGCAACATATACATGTTGGCAGt                       <  1:3435945/93‑1 (MQ=255)

                                                                  gtgCGGACAAATATCCGGATAAACACACGACAAACGGCGACAAGTTTGTTCAGCGCGTGGCGATTTGCAGCGCAACATATACATGTTGGCAGt                       <  2:4337235/93‑1 (MQ=255)

                                                                    gCGGACAAATATCCGGATAAACACACGACAAACGGCGACAAGTTTGTTCAGCGCGTGGCGATTTGCAGCGCAACATATACATGTTGGCAGTTg                     <  1:1546307/93‑1 (MQ=255)

                                                                    gCGGACAAATATCCGGATAAACACACGACAAACGGCGACAAGTTTGTTCAGCGCGTGGCGATTTGCAGCGCAACATATACATGTTGGCAGTTg                     <  2:6302581/93‑1 (MQ=255)

                                                                       gACAAATATCCGGATAAACACACGACAAACGGCGACAAGTTTGTTCAGCGCGTGGCGATTTGCAGCGCAACATATACATGTTGGCAGTTGGcc                  <  2:4089917/93‑1 (MQ=255)

                                                                        aCAAATATCCGGATAAACACACGACAAACGGCGACAAGTTTGTTCAGCGCGTGGCGATTTGCAGCGCAACATATACATGTTGGCAGTTGGccc                 <  2:3059503/93‑1 (MQ=255)

                                                                        aCAAATATCCGGATAAACACACGACAAACGGCGACAAGTTTGTTCAGCGCGTGGCGATTTGCAGCGCAACATATACATGTTGGCAGTTGGccc                 <  2:5817400/93‑1 (MQ=255)

                                                                          aaaTATCCGGATAAACACACGACAAACGGCGACAAGTTTGTTCAGCGCGTGGCGATTTGCAGCGCAACATATACATGTTGGCAGTTGGccccc               >  2:6800020/1‑91 (MQ=255)

                                                                          aaaTATCCGGATAAACACACGACAAACGGCGACAAGTTTGTTCAGCGCGTGGCGATTTGCAGCGCAACATATACATGTTGGCAGTTGGCccac               >  1:6549793/1‑93 (MQ=255)

                                                                          aaaTATCCGGATAAACACACGACAAACGGCGACAAGTTTGTTCAGCGCGTGGCGATTTGCAGCGCAACATATACATGTTGGCAGTTGGCccac               <  2:6966734/93‑1 (MQ=255)

                                                                             tctCCGGATAAACACACGACAAACGGCGGCAAGTTTGTTCAGCGCGTGGCGATTTGCAGCGCAACATATACATGTTGGCAGTTGGCCCACCaa            <  2:2404809/91‑1 (MQ=255)

                                                                             tatCCGGATAAACACACGACAAACGGCGACAAGTTTGTTCAGCGCGTGGCGATTTGCAGCGCAACATATACATGTTGGCAGTTGGCCCACCaa            >  1:7032515/1‑93 (MQ=255)

                                                                               tCCGGATAAACACACGACAAACGGCGACAAGTTTGTTCAGCGCGTGGCGATTTGCAGCGCAACATATACATGTTGGCAGTTGGCCCACCAAGa          <  1:2706430/93‑1 (MQ=255)

                                                                               tCCGGATAAACACACGACAAACGGCGACAAGTTTGTTCAGCGCGTGGCGATTTGCAGCGCAACATATACATGTTGGCAGTTGGCCCACCAAGa          >  1:367706/1‑93 (MQ=255)

                                                                                ccGGATAAACACACGACAAACGGCGACAAGTTTGTTCAGCGCGTGGCGATTTGCAGCGCAACATATACATGTTGGCAGTTGGCCCACCAAGAt         <  1:840567/93‑1 (MQ=255)

                                                                                ccGGATAAACACACGACAAACGGCGACAAGTTTGTTCAGCGCGTGGCGATTTGCAGCGCAACATATACATGTTGGCAGTTGGCCCACCAAGAt         <  1:5405234/93‑1 (MQ=255)

                                                                                ccGGATAAACACACGACAAACGGCGACAAGTTTGTTCAGCGCGTGGCGATTTGCAGCGCAACATATACATGTTGGCAGTTGGCCCACCAAGAt         <  2:5498943/93‑1 (MQ=255)

                                                                                   gATAAACACACGACAAACGGCGACAAGTTTGTTCAGCGCGTGGCGATTTGCAGCGCAACATATACATGTTGGCAGTTGGCCCACCAAGATCgg      >  1:1368081/1‑93 (MQ=255)

                                                                                    aTAAACACACGACAAACGGCGACAAGTTTGTTCAGCGCGTGGCGATTTGCAGCGCAACATATACATGTTGGCAGTTGGCCCACCAAGATCGGa     >  2:7885480/1‑93 (MQ=255)

                                                                                     tAAACACACGACAAACGGCGACAAGTTTGTTCAGCGCGTGGCGATTTGCAGCGCAACATATACATGTTGGCAGTTGGCCCACCAAGATCGGaa    >  2:5749001/1‑93 (MQ=255)

                                                                                     tAAACACACGACAAACGGCGACAAGTTTGTTCAGCGCGTGGCGATTTGCAGCGCAACATATACATGTTGGCAGTTGGCCCACCAAGATCGGaa    >  2:735658/1‑93 (MQ=255)

                                                                                     tAAACACACGACAAACGGCGACAAGTTTGTTCAGCGCGTGGCGATTTGCAGCGCAACATATACATGTTGGCAGTTGGCCCACCAAGATCGGaa    >  1:3018423/1‑93 (MQ=255)

                                                                                      aaaCACACGACAAACGGCGACAAGTTTGTTCAGCGCGTGGCGATTTGCAGCGCAACATATACATGTTGGCAGTTGGCCCACCAAGATCGGaaa   <  1:1019369/93‑1 (MQ=255)

                                                                                      aaaCACACGACAAACGGCGACAAGTTTGTTCAGCGCGTGGCGATTTGCAGCGCAACATATACATGTTGGCAGTTGGCCCACCAAGATCGGaaa   >  1:5523311/1‑93 (MQ=255)

                                                                                      aaaCACACGACAAACGGCGACAAGTTTGTTCAGCGCGTGGCGATTTGCAGCGCAACATATACATGTTGGCAGTTGGCCCACCAAGATCGGaaa   <  2:7818433/93‑1 (MQ=255)

                                                                                       aaCACACGACAAACGGCGACAAGTTTGTTCAGCGCGTGGCGATTTGCAGCGCAACATATACATGTTGGCAGTTGGCCCACCAAGATCGGAAAt  >  2:6667108/1‑93 (MQ=255)

TTTAATGCCTTTCAGATCACGCGCACGGCGATAGAGGTTGATCGTTGGTTCGTGGTTCGTGTCCATGTGCGGACAAATATCCGGATAAACGCACGACAAACGGCGACAAGTTTGTTCAGCGCGTGGCGATTTGCAGCGCAACATATACATGTTGGCAGTTGGCCCACCAAGATCGGAAAT  >  NC_000913/3157700‑3157879

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 28 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`