Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,162,333 A→G Y553H (TAT→CAT)  parC ← DNA topoisomerase IV subunit A

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,162,3330AG99.6% 804.1 / NA 253Y553H (TAT→CAT) parCDNA topoisomerase IV subunit A
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base G (77/175);  minor base C (0/1);  total (77/176)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 318 total aligned reads displayed.

CAACGGTCGCCCCAGGCGGCAACGTTAATTTGCCGGTGAGCGGCTCGCCCTGACCACGCGCCGACGGCAGCGTAATCGGGTCAATGGCATAGCTACGACCGGTGGAATCAACAAACACTACCGGTTGGTTGCTCTTACCTTTCACCGCCGCTTTGAAGCTATCACCCGCTTTATAATTCA  >  NC_000913/3162243‑3162422
                                                                                          |                                                                                         
  aCGGTCGCCCCAGGCGGCAACGTTAATTTGCCGGTGAGCGGCTCGCCCTGACCACGCGCCGACGGCAGCGTAATCGGGGCAATGGCAAGGCTa                                                                                       >  1:182691/1‑93 (MQ=255)
   cGGTCGCCCCAGGCGGCAACGTTAATTTGCCGGTGAGCGGCTCGCCCTGACCACGCGCCGACGGCAGCGTAATCGGGTCAATGGCATGGCTAc                                                                                      <  2:4168552/93‑1 (MQ=255)
   cGGTCGCCCCAGGCGGCAACGTTAATTTGCCGGAGAGCGGCTCGCCCTGACCACGCGCCGACGGCAGCGTAATCGGGTCAATGGCATGGCTAc                                                                                      <  1:7644651/93‑1 (MQ=255)
     gTCGCCCCAGGCGGCAACGTTAATTTGCCGGTGAGCGGCTCGCCCTGACCACGCGCCGACGGCAGCGTAATCGGGTCAATGGCATGGCTACGa                                                                                    >  2:2997874/1‑93 (MQ=255)
     gTCGCCCCAGGCGGCAACGTTAATTTGCCGGTGAGCGGCTCGCCCTGACCACGCGCCGACCGCCGCGTAATCGGGTCAATGGCATGGCTACGa                                                                                    >  1:4242104/1‑93 (MQ=255)
      tCGCCCCAGGCGGCAACGTTAATTTGCCGGTGAGCGGCTCGCCCTGACCACGCGCCGACGGCAGCGTAATCGGGTCAATGGCATGGCTACGAc                                                                                   <  2:4004349/93‑1 (MQ=255)
      gcGCCCCAGGCGGCAACGTTAATTTGCCGGTGAGCGGCTCGCCCTGACCACGCGCCGACGGCAGCGTAATCGGGTCAATGGCATGGCTACGAc                                                                                   <  1:5029021/92‑1 (MQ=255)
       cGCCCCAGGCGGCAACGTTAATTTGCCGGTGAGCGGCTCGCCCTGACCACGCGCCGACGGCAGCGTAATCGGGTCAATGGCATGGCTACGAcc                                                                                  <  2:6434856/93‑1 (MQ=255)
        gCCCCAGGCGGCAACGTTAATTTGCCGGTGAGCGGCTCGCCCTGACCACGCGCCGACGGCAGCGTAATCGGGGCAATGGCATGGGTACGGCCg                                                                                 >  2:3482421/1‑93 (MQ=255)
         ccccAGGCGGCAACGTTAATTTGCCGGTGAGCGGCTCGCCCTGACCACGCGCCGACGGCAGCGTAATCGGGTCAATGGCATGGCTACGACCgg                                                                                <  1:6724944/93‑1 (MQ=255)
         ccccAGGCGGCAACGTTAATTTGCCGGTGAGCGGCTCGCCCTGACCACGCGCCGACGGCAGCGTAATCGGGTCAATGGCATGGCTACGACCgg                                                                                <  1:2014314/93‑1 (MQ=255)
         ccccAGGCGGCAACGTTAATTTGCCGGTGAGCGGCTCGCCCTGACCACGCGCCGACGGCAGCGTAATCGGGGCAATGGCATGGCTAAGACCgg                                                                                >  1:1038965/1‑93 (MQ=255)
          cccAGGCGGCAACGTTAATTTGCCGGTGAGCGGCTCGCCCTGACCACGCGCCGACGGCAGCGTAATCGGGTCAATGGCATGGCTACGACCGGt                                                                               <  1:4450014/93‑1 (MQ=255)
          cccAGGCGGCAACGTTAATTTGCCGGTGAGCGGCTCGCCCTGACCACGCGCCGACGGCAGCGTAATCGGGTCAATGGCATGGCTACGACCGGt                                                                               <  1:7735252/93‑1 (MQ=255)
           ccAGGCGGCAACGTTAATTTGCCGGTGAGCGGCTCGCCCTGACCACGCGCCGACGGCAGCGTAATCGGGTCAATGGCATGGCTACGACCGGTg                                                                              <  2:1164190/93‑1 (MQ=255)
             aGGCGGCAACGTTAATTTGCCGGTGAGCGGCTCGCCCTGACCACGCGCCGACGGCAGCGTAATCGGGTCAATGGCATGGCTACGACCGGTGGa                                                                            <  1:6161098/93‑1 (MQ=255)
             aGGCGGCAACGTTAATTTGCCGGTGAGCGGCTCGCCCTGACCACGCGCCGACGGCAGCGTAATCGGGTCAATGGCATGGCTACGACCGGTGGa                                                                            <  1:5756862/93‑1 (MQ=255)
             aGGCGGCAACGTTAATTTGCCGGTGAGCGGCTCGCCCTGACCACGCGCCGACGGCAGCGTAATCGGGGCAATGGCATGGCTACGACCGGGGGa                                                                            >  1:1107040/1‑93 (MQ=255)
              ggcggcAACGTTAATTTGCCGGTGAGCGGCTCGCCCTGACCACGCGCCGACGGCAGCGTAATCGGGTCAATGGCATGGCTACGACCGggggga                                                                           >  2:6509784/1‑91 (MQ=255)
              ggcggcAACGTTAATTTGCCGGTGAGCGGCTCGCCCTGACCACGCGCCGACGGCAGCGTAATCGGGTCAATGGCATGGCTACGACCGGTGGaa                                                                           <  2:1812447/93‑1 (MQ=255)
               gcggcAACGTTAATTTGCCGGTGAGCGGCTCGCCCTGACCACGCGCCGACGGCAGCGTAATCGGGGCAATGGCCTGGCTACGACCGGTGGAAt                                                                          >  1:6843380/1‑93 (MQ=255)
                cggcAACGTTAATTTGCCGGTGAGCGGCTCGCCCTGACCACGCGCCGACGGCAGCGTAATCGGGTCAATGGCATGGCTACGACCGGTGGAATc                                                                         <  1:6457417/93‑1 (MQ=255)
                cggcAACGTTAATTTGCCGGTGAGCGGCTCGCCCTCACCACGCGCCGACGGCATCGTCATCCGGTAAATTGCATAATTATGACCGGGGGAATc                                                                         >  2:6795606/1‑93 (MQ=255)
                  gcAACGTTAATTTGCCGGTGAGCGGCTCGCCCTGACCACGCGCCGACGGCAGCGTAATCGGGGCAATGGCATGGCTACGACCGGTGGAATcaa                                                                       >  2:7222354/1‑93 (MQ=255)
                    aaCGTTAATTTGCCGGTGAGCGGCTCGCCCTGACCACGCGCCGACGGCAGCGTAATCGGGGCAATGGCATGGCTCCCACCGGGGGAATcaaca                                                                     >  2:1582759/1‑93 (MQ=255)
                    aaCGTTAATTTGCCGGTGAGCGGCTCGCCCTGACCACGCGCCGACGGCAGCGTAATCGGGGCAATGGCATGGCTACGACCGGGGGGATgaaca                                                                     >  1:3430738/1‑93 (MQ=255)
                     aCGTTAATTTGCCGGTGAGCGGCTCGCCCTGACCCCGCGCCGACGGCAGCGGAATCGGGGCAATGGCATGGCTACGACCGGGGGAATcaacaa                                                                    >  2:7488417/1‑93 (MQ=255)
                     aCGTTAATTTGCCGGTGAGCGGCTCGCCCTGACCACGCGCCGACGGCAGCGTAATCGGGTCAATGGCATGGCTACGACCGGTGGAATcaacaa                                                                    <  1:7456071/93‑1 (MQ=255)
                     aCGTTAATTTGCCGGTGAGCGGCTCGCCCTGACCACGCGCCGACGGCAGCGTAATCGGGTCAATGGCATGGCTACGACCGGTGGAATcaacaa                                                                    >  1:1372299/1‑93 (MQ=255)
                       gTTAATTTGCCGGTGAGCGGCTCGCCCTGACCACGCGCCGACGGCAGCGTAATCGGGTCAATGGCATGGCTACGACCGGTGGAATCaacaaac                                                                  <  2:7578058/93‑1 (MQ=255)
                       gTTAATTTGCCGGTGAGCGGCTCGCCCTGACCACGCGCCGACGGCAGCGTAATCGGGTCAATGGCATGGCTACGACCGGTGGAATCaacaaac                                                                  <  2:1346592/93‑1 (MQ=255)
                       gTTAATTTGCCGGTGAGCGGCTCGCCCTGACCACGCGCCGACGGCAGCGTAATCGGGTCAATGGCATGGCTACGACCGGGGGGATCaacaaac                                                                  >  2:2881883/1‑93 (MQ=255)
                       gTTAATTTGCCGGTGAGCGGCTCGCCCTGACCACGCGCCGACGGCAGCGTAATCGGGTCAATGGCATGGCTACGACCGGGGGGATCaacaaaa                                                                  >  1:3142382/1‑92 (MQ=255)
                        ttAATTTGCCGGTGAGCGGCTCGCCCTGACCACGCGCCGACGGCAGCGTAATCGGGTCAATGGCATGGCTACGACCGGTGGAATCaacaaaca                                                                 <  2:6748291/93‑1 (MQ=255)
                        ttAATTTGCCGGTGAGCGGCTCGCCCTGACCACGCGCCGACGGCAGCGTAATCGGGTCAATGGCATGGCTACGACCGGTGGAATCaacaaaca                                                                 <  2:1448756/93‑1 (MQ=255)
                           aTTTGCCGGTGAGCGGCTCGCCCTGACCACGCGCCGCCGGCAGCGTAATCGGGTCAATGGCATGGCTACGACCGGGGGAATCAACAAacacca                                                              >  1:7305055/1‑91 (MQ=255)
                           aTTTGCCGGTGAGCGGCTCGCCCTGACCACGCGCCGACGGCAGCGTAATCGGGTCAATGGCATGGCTACGACCGGTGGAATCAACAAACACTa                                                              <  2:1678902/93‑1 (MQ=255)
                            tttGCCGGTGAGCGGCTCGCCCTGACCACGCGCCGACGGCAGCGTAATCGGGTCAATGGCATGGCTACGACCGGTGGAATCAACAAACACTAc                                                             <  1:4809901/93‑1 (MQ=255)
                             ttGCCGGTGAGCGGCTCGCCCTGACCACGCGCCGACGGCAGCGTAATCGGGTCAATGGCATGGCTACGACCGGTGGAATCAACAAACACTAcc                                                            <  1:1953671/93‑1 (MQ=255)
                                ccGGTGAGCGGCTCGCCCTGACCACGCGCCGACGGCAGCGTAATCGGGTCAATGGCATGGCTACGACCCGTGGAATCAACAAAAACCACCggt                                                         >  1:7117720/1‑93 (MQ=255)
                                 cGGTGAGCGGCTCGCCCCGACCACGCGCCGACGGCAGCGGAATCGGGGCAATGGCATGGCTACGACCGGGGGGATCAAAAAAAACCACCgggt                                                        >  2:7023017/1‑91 (MQ=255)
                                  ggTGAGCGGCTCGCCCTGACCACGCGCCGACGGCAGCGTAATCGGGTCAATGGCATGGCTACGACCGGTGGAATCAACAAACACTACCggttg                                                       <  2:1906645/93‑1 (MQ=255)
                                  ggTGAGCGGCTCGCCCTGACCACGCGCCGACGGCAGCGTAATCGGGTCAATGGCATGGCTACGACCGGTGGAATCAACAAACACTACCggttg                                                       <  1:4095568/93‑1 (MQ=255)
                                    tGAGCGGCTCGCCCTGACCACGCGCCGACGGCAGCGTAATCGGGTCAATGGCATGGCTACGACCGGTGGAATCAACAAACACTACCggttggt                                                     >  1:5501878/1‑93 (MQ=255)
                                    tGAGCGGCTCGCCCTGACCACGCGCCGACGGCAGCGTAATCGGGTCAATGGCATGGCTACGACCGGTGGAATCAACAAACACTACCggttggt                                                     <  2:7694138/93‑1 (MQ=255)
                                    tGAGCGGCTCGCCCTGACCACGCGCCGACGGCAGCGTAATCGGGGCAATGGCATGGCTACGACCGGTGGGATCAAAAAACACTACCgggtggt                                                     >  2:3169580/1‑93 (MQ=255)
                                     gAGCGGCTCGCCCTGACCACGCGCCGACGGCAGCGTAATCGGGTCAATGGCATGGCTACGACCGGTGGAATCAACAAACACTACCggttggtt                                                    <  1:6487240/93‑1 (MQ=255)
                                       gCGGCTCGCCCCGACCACGCGCCGACGGCAGCGTAATCGGGGCAAAGGGATGGCTACGACCCGTGGGATCAACAAACACCACCCGGTGGGTGc                                                  >  2:42847/1‑93 (MQ=255)
                                          gCTCGCCCTGACCACGCGCCGACGGCAGCGTAATCGGGTCAATGGCATGGCTACGACCGGTGGAATCAACAAACACTACCGGTTGGTTGctct                                               >  2:2472584/1‑93 (MQ=255)
                                            tCGCCCTGACCACGCGCCGACGGCAGCGTAATCGGGTCAATGGCATGGCTACGACCGGTGGAATCAACAAACACTACCGGTTGGTTGCTCTTa                                             >  1:5293501/1‑93 (MQ=255)
                                            tCGCCCTGACCACGCGCCGACGGCAGCGTAATCGGGTCAATGGCATGGCTACGACCGGTGGAATCAACAAACACTACCGGTTGGTTGCTCTTa                                             <  2:6137810/93‑1 (MQ=255)
                                               cccTGACCACGCGCCGACGGCAGCGTAATCGGGTCAATGGCATGGCTACGACCGGTGGCATCAACAAACACTACCGGTTGGTTGCTCTTACCt                                          >  2:6848246/1‑93 (MQ=255)
                                                ccTGACCACGCGCCGACGGCAGCGTAATCGGGTCAATGGCATGGCTACGACCGGTGGAATCAACAAACACTACCGGTTGGTTGCTCTTACCtt                                         <  1:2064242/93‑1 (MQ=255)
                                                ccTGACCACGCGCCGACGGCAGCGTAATCGGGTCAATGGCATGGCTACGACCGGTGGAATCAACAAACACTACCGGTTGGTTGCTCTTACCtt                                         <  1:7005174/93‑1 (MQ=255)
                                                 cTGACCACGCGCCGACGGCAGCGTAATCGGGTCAATGGCATGGCTACGACCGGTGGAATCAACAAACACTACCGGTTGGTTGCTCTTACCttt                                        >  1:5370019/1‑93 (MQ=255)
                                                 cTGACCACGCGCCGACGGCAGCGTAATCGGGTCAATGGCATGGCTACGACCGGTGGAATCAACAAACACTACCGGTTGGTTGCTCTTACCttt                                        >  2:2765769/1‑93 (MQ=255)
                                                 cTGACCACGCGCCGACGGCAGCGTAATCGGGTCAATGGCATGGCTACGACCGGTGGAATCAACAAACACTACCGGTTGGTTGCTCTTACCttt                                        >  2:5286329/1‑93 (MQ=255)
                                                  tGACCACGCGCCGACGGCAGCGTAATCGGGTCAATGGCATGGCTACGACCGGTGGAATCAACAAACACTACCGGTTGGTTGCTCTTACCTTTc                                       >  2:2421594/1‑93 (MQ=255)
                                                  tGACCACGCGCCGACGGCAGCGGAATCGGGTCAATGGCATGGCTACGACCGGTGGCATCAACAAACACTACCGGTTGGTTGGTCTTCCCttta                                       >  2:6691169/1‑92 (MQ=255)
                                                    aCCACGCGCCGACGGCAGCGTAATCGGGTCAATGGCATGGCTACGACCGGTGGAATCAACAAACACTACCGGTTGGTTGCTCTTACCTTTCAc                                     <  1:1609312/93‑1 (MQ=255)
                                                    aCCACGCGCCGACGGCAGCGTAATCGGGTCAATGGCATGGCTACGACCGGTGGAATCAACAAACACTACCGGTTGGTTGCTCTTACCTTTCAc                                     >  2:5768267/1‑93 (MQ=255)
                                                    aCCACGCGCCGACGGCAGCGTAATCGGGTCAATGGCATGGCTACGACCGGTGGAATCAACAAACACTACCGGTTGGTTGCTCTTACCTTTCAc                                     <  1:302462/93‑1 (MQ=255)
                                                       aCGCGCCGACGGCAGCGTAATCGGGTCAATGGCATGGCTACGACCGGTGGAATCAACAAACACTACCGGTTGGTTGCTCTTACCTTTCAccgc                                  <  1:326403/93‑1 (MQ=255)
                                                       aCGCGCCGACGGCAGCGTAATCGGGTCAATGGCATGGCTACGACCGGTGGAATCAACAAACACTACCGGTTGGTTGCTCTTACCTTTCAccgc                                  <  1:3897559/93‑1 (MQ=255)
                                                        cgcgCCGACGGCAGCGTAATCGGGTCAATGGCATGGCTACGACCGGTGGAATCAACAAACACTCCCGGTTGGTTGCTCTTACCTTTCAccgcc                                 <  1:4981186/93‑1 (MQ=255)
                                                        cgcgCCGACGGCAGCGTAATCGGGTCAATGGCATGGCTACGACCGGTGGAATCAACAAACACTACCGGTTGGTTGCTCTTACCTTTCAccgcc                                 <  2:4680489/93‑1 (MQ=255)
                                                         gcgcCGACGGCAGCGTAATCGGGTCAATGGCATGGCTACGACCGGTGGAATCAACAAACACTACCGGTTGGTTGCTCTTACCTTTCAccgccg                                <  2:2655689/93‑1 (MQ=255)
                                                            ccGACGGCAGCGTAATCGGGTCAATGGCATGGCTACGACCGGTGGAATCAACAAACACTACCGGTTGGTTGCTCTTACCTTTCACCGCCGCtt                             <  1:3611675/93‑1 (MQ=255)
                                                             cGACGGCAGCGTAATCGGGTCAATGGCATGGCTACGACCGGTGGAATCAACAAACACTACCGGTTGGTTGCTCTTACCTTTCACCGCCGCttt                            <  2:5443432/93‑1 (MQ=255)
                                                              gACGGCAGCGTAATCGGGTCAATGGCATGGCTACGACCGGTGGAATCAAGAAACACTACCGGTTGGTTGCTCTTACCTTTCACCGCCGCTTTg                           >  2:783266/1‑93 (MQ=255)
                                                               aCGGCAGCGTAATCGGGTCAATGGCATGGCTACGACCGGTGGAATCAACAAACACTACCGGTTGGTTGCTCTTACCTTTCACCGCCGCTTTGa                          <  2:5949456/93‑1 (MQ=255)
                                                               aCGGCAGCGTAATCGGGTCAATGGCATGGCTACGACCGGTGGAATCAACAAACACTACCGGTTGGTTGCGCTTACCTTTCACCGCCGCTTTGa                          >  2:4362278/1‑93 (MQ=255)
                                                                 ggCAGCGTAATCGGGTCAATGGCATGGCTACGACCGGTGGAATCAACAAACACTACCGGTTGGTTGCTCTTACCTTTCACCGCCGCTTTGAAg                        >  2:3601579/1‑93 (MQ=255)
                                                                 ggCAGCGTAATCGGGTCAATGGCATGGCTACGACCGGTGGAATCAACAAACACTACCGGTTGGTTGCTCTTACCTTTCACCGCCGCTTTGAAg                        >  2:7308340/1‑93 (MQ=255)
                                                                    aGCGTAGTCGGTTCAATGGCATGGCTACGACCGGTGGAATCAACAAACACTACCGGTTGGTTGCTCTTACCTTTCACCGCCGCTTTGAAGCTa                     <  2:5111964/93‑1 (MQ=255)
                                                                    aGCGTAATCGGGTCAATGGCATGGCTACGACCGGTGGAATCAACAAACACTACCGGTTGGTTGCTCTTACCTTTCACCGCCGCTTTGAAGCTa                     >  2:5611013/1‑93 (MQ=255)
                                                                      cGTAATCGGGTCAATGGCATGGCTACGACCGGTGGAATCAACAAACACTACCGGTTGGTTGCTCTTACCTTTCACCGCCGCTTTGAAGCTATc                   <  1:2787681/93‑1 (MQ=255)
                                                                       gTAATCGGGTCAATGGCATGGCTACGACCGGTGGAATCAACAAACACTACCGGTTGGTTGCTCTTACCTTTCACCGCCGCTTTGAAGCTATCa                  <  2:3880947/93‑1 (MQ=255)
                                                                        tAATCGGGTCAATGGCATGGCTACGACCGGTGGAATCAACAAACACTACCGGTTGGTTGCTCTTACCTTTCACCGCCGCTTTGAAGCTATCAc                 <  1:4719718/93‑1 (MQ=255)
                                                                        tAATCGGGTCAATGGCATGGCTACGACCGGTGGAATCAACAAACACTACCGGTTGGTTGCTCTTACCTTTCACCGCCGCTTTGAAGCTATCAc                 <  2:7729328/93‑1 (MQ=255)
                                                                         aaTCGGGTCAATGGCATGGCTACGACCGGTGGAATCAACAAACACTACCGGTTGGTTGCTCTTACCTTTCACCGCCGCTTTGAAGCTATCAcc                >  2:2266981/1‑93 (MQ=255)
                                                                           tCGGGTCAATGGCATGGCTACGACCGGTGGAATCAACAAACACTACCGGTTGGTTGCTCTTACCTTTCACCGCCGCTTTGAAGCTATCACCCg              >  1:1529992/1‑93 (MQ=255)
                                                                             gggTCAATGGCATGGCTACGACCGGTGGAATCAACAAACACTACCGGTTGGTTGCTCTTACCTTTCACCGCCGCTTTGAAGCTATCACCCGCt            >  2:3833182/1‑93 (MQ=255)
                                                                             gggTCAATGGCATGGCTACGACCGGTGGAATCAACAAACACTACCGGTTGGTTGCTCTTACCTTTCACCGCCGCTTTGAAGCTATCACCCGCt            >  1:7260140/1‑93 (MQ=255)
                                                                                tCAATGGCATGGCTACGACCGGTGGAATCAACAAACACTACCGGTTGGTTGCTCTTACCTTTCACCGCCGCTTTGAAGCTATCACCCGCTTta         >  1:5602400/1‑93 (MQ=255)
                                                                                gcAATGGCATGGCTACGACCGGTGGAATCAACAAACACTACCGGTTGGTTGCTCTTACCTTTCACCGCCGCTTTGAAGCTATCACCCGCTTta         <  2:2092463/92‑1 (MQ=255)
                                                                                   atggcatggcTACGACCGGTGGAATCAACAAACACTACCGGTTGGTTGCTCTTACCTTTCACCGCCGCTTTGAAGCTATCACCCGCTTTATaa      >  2:4919397/1‑93 (MQ=255)
                                                                                   atggcatggcTACGACCGGTGGAATCAACAAACACTACCGGTTGGTTGCTCTTACCTTTCACCGCCGCTTTGAAGCTATCACCCGCTTTATaa      <  2:3371743/93‑1 (MQ=255)
                                                                                    tGGCATGGCTACGACCGGTGGAATCAACAAACACTACCGGTTGGTTGCTCTTACCTTTCACCGCCGCTTTGAAGCTATCACCCGCTTTATAAt     <  1:2365180/93‑1 (MQ=255)
                                                                                    tGGCATGGCTACGACCGGTGGAATCAACAAACACTACCGGTTGGTTGCTCTTACCTTTCACCGCCGCTTTGAAGCTATCACCCGCTTTATAAt     <  1:2672056/93‑1 (MQ=255)
                                                                                    tGGCATGGCTACGACCGGTGGAATCAACAAACACTACCGGTTGGTTGCTCTTACCTTTCACCGCCGCTTTGAAGCTATCACCCGCTTTATAAt     >  2:3257434/1‑93 (MQ=255)
                                                                                     ggCATGGCTACGACCGGTGGAATCAACAAACACTACCGGTTGGTTGCTCTTACCTTTCACCGCCGCTTTGAAGCTATCACCCGCTTTATAAtt    <  1:3110419/93‑1 (MQ=255)
                                                                                     ggCATGGCTACGACCGGTGGAATCAACAAACACTACCGGTTGGTTGCTCTTACCTTTCACCGCCGCTTTGAAGCTATCACCCGCTTTATAAtt    <  2:1088774/93‑1 (MQ=255)
                                                                                     ggCATGGCTACGACCGGTGGAATCAACAAACACTACCGGTTGGTTGCTCTTACCTTTCACCGCCGCTTTGAAGCTATCACCCGCTTTATAAtt    <  1:903031/93‑1 (MQ=255)
                                                                                      gCATGGCTACGACGGGTGTAATCAACAAACACTACCGGTTGGTTGCTCTTACCTTTCACCGCCGCTTTGAAGCTATCACCCGCTTTATAATTc   <  1:5450686/93‑1 (MQ=255)
                                                                                      gCATGGCTACGACCGGTGGAATCAACAAACACTACCGGTTGGTTGCTCTTACCTTTCACCGCCGCTTTGAAGCTATCACCCGCTTTATAATTc   <  1:4693378/93‑1 (MQ=255)
                                                                                      gCATGGCTACGACCGGGGGAATCAACAAACACTACCGGTTGGTTGCTCTTACCTTTCACCGCCGCTTTGAAGCTATCGCCCGCTTTATAATTc   <  2:2186076/93‑1 (MQ=255)
                                                                                       cATGGCTACGACCGGTGGAATCAACAAACCCTACCGGTTGGTTGCTCTTACCTTTCCCCGCCGCTTTGAAGCTATCACCCGCTTTATAATTCa  <  2:4547089/93‑1 (MQ=255)
                                                                                       cATGGCTACGACCGGTGGAATCAACAAACACTACCGGTTGGTTGCTCTTACCTTTCACCGCCGCTTTGAAGCTATCACCCGCTTTATAATTCa  <  1:2144162/93‑1 (MQ=255)
                                                                                       cATGGCTACGACCGGTGGAATCAACAAACACTACCGGTTGGTTGCTCTTACCTTTCACCGCCGCTTTGAAGCTATCACCCGCTTTATAATTCa  <  1:2277800/93‑1 (MQ=255)
                                                                                          |                                                                                         
CAACGGTCGCCCCAGGCGGCAACGTTAATTTGCCGGTGAGCGGCTCGCCCTGACCACGCGCCGACGGCAGCGTAATCGGGTCAATGGCATAGCTACGACCGGTGGAATCAACAAACACTACCGGTTGGTTGCTCTTACCTTTCACCGCCGCTTTGAAGCTATCACCCGCTTTATAATTCA  >  NC_000913/3162243‑3162422

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑