BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,212,918	A→G	V66A (GTC→GCC)	ygjF ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,212,918	0	A	G	99.4%	995.1 / ‑3.5	322	V66A (GTC→GCC)	ygjF	hypothetical protein
Reads supporting (aligned to +/- strand): ref base A (1/1); new base G (174/146); total (175/147)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.40e-01

Only 100 of 340 total aligned reads displayed.

TTTTTTCAATCAGCTTACGCCCGCCTGCGTGTAGCTCCTGCTTTGAAACTTCATTGGCTTGCACCGTTGGACGGTCTACCAGTTTGGTGACGCCACAACGATAATCCAGCAGATGCTGTGCCTCCTGCGGCTTCAACTGACGGTCGGTAAACCCGGCCTGATATATCACCTTCCAGAAG  >  NC_000913/3212829‑3213007

 tttttCAATCAGCTTACGCCCGCCTGCGTGTAGCTCCTGCTTTGAAACTTCATTGGCTTGCACCGTTGGACGGTCTACCAGGTTGGTgccgcc                                                                                       >  1:3089001/1‑93 (MQ=255)

 tttttCAATCAGCTTACGCCCGCCTGCGTGTAGCTCCTGCTTTGAAACTTCATTGGCTTGCACCGCTGGACGGTCTACCAGTTTGGTGGCGcc                                                                                       >  2:1322050/1‑93 (MQ=255)

   tttCAATCAGCTTACGCCCGCCTGCGTGTAGCTCCTGCTTTGAAACTTCATTGGCTTGCACCGTTGGACGGTCTACCAGTTTGGTGGCGCcac                                                                                     <  2:6303801/93‑1 (MQ=255)

   tttCAATCAGCTGACGCCCGCCTGCGTGTAGCTCCTGCTTTGAAACTTCATTGGCTTGCACCGTTGGACGGTCTACCAGTTTGGTGGCGCcac                                                                                     <  2:1635227/93‑1 (MQ=255)

    ttCAATCAGCTTACGCCCGCCTGCGTGTAGCTCCTGCTTTGAAACTTCATTGGCTTGCACCGTTGGACGGTCTACCAGTTTGGTGGCGCcaca                                                                                    <  1:4389849/93‑1 (MQ=255)

      cAATCAGCTTACGCCCGCCTGCGTGTAGCTCCTGCTTTGAAACTTCATTGGCTTGCACCGTTGGACGGTCTACCAGTTTGGTGGCGCCACAAc                                                                                  <  1:157806/93‑1 (MQ=255)

      cAATCAGCTTACGCCCGCCTGCGTGTAGCTCCTGCTTTGAAACTTCATTGGCTTGCACCGTTGGACGGTCTACCAGTTTGGTGGCGCCACAAc                                                                                  <  2:5794442/93‑1 (MQ=255)

      cAATCAGCTTACGCCCGCCTGCGTGTAGCTCCTGCTTTGAAACTTCATTGGCTTGCACCGTTGGACGGCGTACCACTTTGGTGGCGCCACAAc                                                                                  >  1:2658789/1‑93 (MQ=255)

       aaTCAGCTTACGCCCGCCTGCGTGTAGCTCCTGCTTTGAAACTTCATTGGCTTGCACCGTTGGACGGTCTACCAGTTTGGTGGCGCCCCAACg                                                                                 >  1:2421382/1‑93 (MQ=255)

       aaTCAGCTTACGCCCGCCTGCGTGTAGCTCCTGCTTTGAAACTTCATTGGCTTGCACCGTTGGACGGTCTACCAGTTTGGTGGCGCCACAACg                                                                                 >  1:3177937/1‑93 (MQ=255)

        aTCAGCTTACGCCCGCCTGCGTGTAGCTCCTGCTTTGAAACTTCATTGGCTTGCACCGTTGGACGGTCTACCAGTTTGGTGGCGCCACAACGa                                                                                <  1:1838528/93‑1 (MQ=255)

         tCAGCTTACGCCCGCCTGCGTGTAGCTCCTGCTTTGAAACTTGATTGGCTTGCACCGTTGGACGGTCTACCAGTTTGGTGGCGCCACGACGAt                                                                               >  1:1955037/1‑93 (MQ=255)

         tCAGCTTACGCCCGCCTGCGTGTAGCTCCTGCTTTGAAACTTCATTGGCTTGCACCGTTGGACGGTCTACCAGTTTGGTGGCGCCACAACGAt                                                                               <  2:1935909/93‑1 (MQ=255)

         tCAGCTTACGCCCGCCTGCGTGTAGCTCCTGCTTTGAAACTTCATTGGCTTGCACCGTTGGACGGTCTACCAGTTTGGTGGCGCCACAACGAt                                                                               >  2:2009629/1‑93 (MQ=255)

         tCAGCTTACGCCCGCCTGCGTGTAGCTCCTGCTTTGAAACTTCATTGGCTTGCACCGTTGGACGGTCTACCAGTTTGGTGGCGCCACAACGAt                                                                               >  2:5079669/1‑93 (MQ=255)

         tCAGCTTACGCCCGCCTGCGTGTAGCTCCTGCTTTGAAACTTCATTGGCTTGCACCGTTGGACGGTCTACCAGTTTGGTGGCGCCACAACGAt                                                                               <  1:5300390/93‑1 (MQ=255)

          cAGCTTACGCCCGCCTGCGTGTAGCTCCTGCTTTGAAACTTCATTGGCTTGCACCGTTGGACGGTCTACCAGGTTGACGGCGAGACAACGATa                                                                              >  1:3771078/1‑93 (MQ=255)

           aGCTTACGCCCGCCTGCGTGTAGCTCCTGCTTTGAGACTTCATTGGCTTGCACCGTTGGACGGTCTACCAGTTTGGTGGCGCCACAACGATaa                                                                             >  2:7830184/1‑93 (MQ=255)

           aGCTTACGCCCGCCTGCGTGTAGCTCCTGCTTTGAAACTTCATTGGCTTGCACCGTTGGACGGTCTACCAGTTTGGTGGCGCCACAACGATaa                                                                             <  2:2731439/93‑1 (MQ=255)

            gCTTACGCCCGCCTGCGTGTAGCTCCTGCTTTGAAACTTCATTGGCTTGCACCGTTGGACGGTCTACCAGTTTGGTGGCGCCACAACGATAAt                                                                            <  2:5309161/93‑1 (MQ=255)

            gCTTACGCCCGCCTGCGTGTAGCTCCTGCTTTGAAACTTCATTGGCTTGCACCGTTGGACGGTCTACCAGTTTGGTGGCGCCACAACGATAAt                                                                            >  2:4826601/1‑93 (MQ=255)

             cTTACGCCCGCCTGCGTGTAGCTCCTGCTTTGAAACTTCATTGGCTTGCACCGTTGGACGGTCTACCAGTTTGGTGGCGCCACAACGATAATc                                                                           <  1:7188867/93‑1 (MQ=255)

              ttACGCCCGCCTGCGTGTAGCTCCTGCTTTGAAACTTCATTGGCTTTCACCGATGGGCGTTCTTTCAGTTTGGTGGAGCCACAAAGATAATcc                                                                          >  1:750546/1‑93 (MQ=255)

              ttACGCCCGCCTGCGTGTAGCTCCTGCTTTGAAACTTCATTGGCTTGCACCGTTGGACGGTCTACCAGTTTGGTGGCGCCACAACGATAATcc                                                                          >  2:4983773/1‑93 (MQ=255)

              ttACGCCCGCCTGCGTGTAGCTCCTGCTTTGAAACTTCATTGGCTTGCACCGTTGGACGGTCTACCAGTTTGGTGGCGCCACAACGATAATcc                                                                          >  1:3531639/1‑93 (MQ=255)

               tACGCCCGCCTGCGTGTAGCTCCTGCTTTGAAACTTCATTGGCTTGCACCGTTGGACGGTCTACCAGTTTGGTGGCGCCACAACGATAATCca                                                                         <  2:2329409/93‑1 (MQ=255)

                 cgcccgccTGCGTGTAGCTCCTGCTTTGAAACTTCATTGGCTTGCACCGTTGGACGGTCTACCAGTTTGGTGGCGCCACAACGATAATCcagc                                                                       <  1:1251944/93‑1 (MQ=255)

                   cccgccTGCGTGTAGCTCCTGCTTTGAAACTTCATTGGCTTGCACCGTTGGACGGTCTACCAGTTTGGTGGCGCCACAACGATAATCcagcag                                                                     >  1:6981660/1‑93 (MQ=255)

                    ccgccTGCGTGTAGCTCCTGCTTTGAAACTTCATTGGCTTGCACCGTTGGACGGTCTACCAGTTTGGTGGCGCCACAACGATAATCCAGCAGa                                                                    >  2:2134854/1‑93 (MQ=255)

                    ccgccTGCGTGTAGCTCCTGCTTTGAAACTTCATTGGCTTGCACCGTTGGACGGTCTACCAGTTTGGTGGCGCCACAACGATAATCCAGCAGa                                                                    <  1:1668731/93‑1 (MQ=255)

                    ccgccTGCGTGTAGCTCCTGCTTTGAAACTTCATTGGCTTGCACCGTTGGACGGTCTACCAGTTTGGTGGCGCCACAACGATAATCCAGCAGa                                                                    >  1:7068422/1‑93 (MQ=255)

                    ccgccTGCGTGTAGCTCCTGCTTTGAAACTTCATTGGCTTGCACCGTTGGACGGTCTACCAGTTTGGTGGCGCCACAACGATAATCCAGCAGa                                                                    <  1:6941038/93‑1 (MQ=255)

                    ccgccTGCGTGTAGCTCCTGCTTCGAAACTTCATTGGCTTGCACCGTTGGACGGTCTACCAGTTTGGTGGCGCCACAACGATAATCCAGCAGa                                                                    <  2:4325285/93‑1 (MQ=255)

                     cgccTGCGTGTAGCTCCTGCTTTGAAACTTCATTGGCTTGCACCGTTGGACGGTCTACCAGTTTGGTGGCGCCACAACGATAATCCAGCAGAt                                                                   <  2:5221423/93‑1 (MQ=255)

                     cgccTGCGTGTAGCTCCTGCTTTGAAACTTCATTGGCTTGCACCGTTGGACGGTCTACCAGTTTGGTGGCGCCACAACGATAATCCAGCAGAt                                                                   >  2:678954/1‑93 (MQ=255)

                     cgccTGCGTGTAGCTCCTGCTTTGAAACTTCATTGGCTTGCACCGTTGGACGGTCTACCAGTTTGGTGGCGCCACAACGATAATCCAGCAGAt                                                                   >  1:6772043/1‑93 (MQ=255)

                       ccTGCGTGTAGCTCCTGCTTTGAAACTTCATTGGCTTGCACCGTTGGACGGTCTACCAGTTTGGTGGCGCCACAACGATAATCCAGCAGATGc                                                                 >  1:926189/1‑93 (MQ=255)

                        cTGCGTGTAGCTCCTGCTTTGAAACTTCATTGGCTTGCACCGTTGGACGGTCTACCAGTTTGGTGGCGCCACAACGATAATCCAGCAGATGCt                                                                <  1:2788159/93‑1 (MQ=255)

                         tGCGTGTAGCTCCTGCTTTGAAACTTCATTGGCTTGCACCGTTGGACGGTCTACCAGTTTGGTGGCGCCACAACGATAATCCAGCAGATGCtg                                                               >  1:2229115/1‑93 (MQ=255)

                         tGCGTGTAGCTCCTGCTTTGAAACTTCATTGGCTTGCACCGTTGGACGGTCTACCAGTTTGGTGGCGCCACAACGATAATCCAGCAGATGCtg                                                               >  1:104442/1‑93 (MQ=255)

                            gtgtAGCTCCTGCTTTGAAACTTCATTGGCTTGCACCGGTGGACGGTCTACCAGTTTGGTGGCGCCCCAACGATAATCCAGCAGATGCTGTGc                                                            >  2:4034909/1‑93 (MQ=255)

                             tgtAGCTCCTGCTTTGAAACTTCATTGGCTTGCACCGTTGGACGGTCTACCAGTTTGGTGGCGCCACAACGATAATCCAGCAGATGCTGTGcc                                                           >  2:1854226/1‑93 (MQ=255)

                              gtAGCTCCTGCTTTGAAACTTCATTGGCTTGCACCGTTGGACGGTCTACCAGTTTGGTGGCGCCACAACGATAATCCAGCAGATGCTGTGcct                                                          <  1:4653101/93‑1 (MQ=255)

                               tAGCTCCTGCTTTGAAACTTCATTGGCTTGCACCGTTGGACGGTCTACCAGTTTGGTGGCGCCCCCACGATAATCCAGCAGATGCTGTGcctc                                                         >  2:79070/1‑93 (MQ=255)

                               tAGCTCCTGCTTTGAAACTTCATTGGCTTGCACCGTTGGACGGTCTACCAGTTTGGTGGCGCCACAACGATAATCCAGCAGATGCTGTGcctc                                                         >  2:3787357/1‑93 (MQ=255)

                               tAGCTCCTGCTTTGAAACTTCATTGGCTTGCACCGTTGGACGGTCTACCAGTTTGGTGGCGCCACAACGATAATCCAGCAGATGCTGTGcctc                                                         <  2:2970218/93‑1 (MQ=255)

                                aGCTCCTGCTTTGAAACTTCATTGGCTTGCACCGTTGGACGGTCTACCAGTTTGGTGGCGCCACAACGATAATCCAGCAGATGCTGTGcctcc                                                        >  1:5920503/1‑93 (MQ=255)

                                aGCTCCTGCTTTGAAACTTCATTGGCTTGCACCGTTGGACGGGCTACCAGTTTGGTGGCGCCACAACGATAATTCAGCAGATGCTGTGcctcc                                                        >  2:5582400/1‑93 (MQ=255)

                                 gCTCCTGCTTTGAAACTTCATTGGCTTGCACCGTTGGACGGTCTACCAGTTTGGTGGCGCCACAACGATAATCCAGCAGATGCTGTGcctcct                                                       >  1:6893088/1‑93 (MQ=255)

                                   tCCTGCTTTGAAACTTCATTGGCTTGCACCGTTGGACGGTCTACCAGTTTGGTGGCGCCACAACGATAATCCAGCAGATGCTGTGCCTCCTGc                                                     >  1:856390/1‑93 (MQ=255)

                                   tCCTGCTTTGAAACTTCATTGGCTTGCACCGTTGGACGGTCTACCAGTTTGGTGGCGCCACAACGATAATCCAGCAGATGCTGTGCCTCCTGc                                                     <  2:7636768/93‑1 (MQ=255)

                                    ccTGCTTTGAAACTTCATTGGCTTGCACCGTTGGACGGTCTACCAGTTTGGTGGCGCCACAACGATAATCCAGCAGATGCTGTGCCTCCTGCg                                                    >  2:543747/1‑93 (MQ=255)

                                     cTGCTTTGAAACTTCATTGGCTTGCACCGTTGGACGGTCTACCAGTTTGGTGGCGCCACAACGATAATCCAGCAGATGCTGTGCCTCCTGCgg                                                   >  1:5231056/1‑93 (MQ=255)

                                       gCTTTGAAACTTCATTGGCTTGCACCGTTGTACGGTCTACCAGTTTGGTGGCGCCACAACGATAATCCAGCAGATGCTGTGCCTCCTGCGGCt                                                 <  2:7729696/93‑1 (MQ=255)

                                        cTTTGAAACTTCATTGGCTTGCACCGTTGGACGGTCTACCAGTTTGGTGGCGCCACAACGATAATCCAGCAGATGCTGTGCCTCCTGCGGCtt                                                >  2:4563583/1‑93 (MQ=255)

                                         tttGAAACTTCATTGGCTTGCACCGTTGGACGGTCTACCAGTTTGGTGGCGCCACAACGATAATCCAGCAGATGCTGTGCCTCCTGCGGCTTc                                               >  1:6650581/1‑93 (MQ=255)

                                          ttGAAACTTCATTGGCTTGCACCGTTGGACGGTCTACCAGTTTGGTGGCGCCACAACGATAATCCAGCAGATGCTGTGCCTCCTGCGGCTTCa                                              >  1:1770152/1‑93 (MQ=255)

                                            taaaCTTCATTGGCTTGCACCGTTGGACGGTCTACCAGTTTGGTGGCGCCACAACGATAATCCAGCAGATGCTGTGCCTCCTGCGGCTTCAAc                                            <  2:6342157/92‑1 (MQ=255)

                                            gAAACTTCATTGGCTTGCACCGTTGGACGGTCTACCAGTTTGGTGGCGCCACAACGATAATCCAGCAGATGCTGTGCCTCCTGCGGCTTCAAc                                            <  1:950705/93‑1 (MQ=255)

                                             aaaCTTCATTGGCTTGCACCGTTGGACGGTCTACCAGTTTGGTGGCGCCACAACGATAATCCAGCAGATGCTGTGCCTCCTGCGGCTTCAACt                                           <  2:6045028/93‑1 (MQ=255)

                                              aaCTTCATTGGCTTGCACCGTTGGACGGTCTACCAGGTTGGTGGCGCCACAACGATAATTCAGCAGATGCTGTGCCTCCTGCGGCTTCAACTg                                          >  1:2364568/1‑93 (MQ=255)

                                               aCTTCATTGGCTTGCACCGTTGGACGGTCTACCAGTTTGGTGGCGCCACAACGATAATCCAGCAGATGCTGTGCCTCCTGCGGCTTCAACTGa                                         <  2:3635120/93‑1 (MQ=255)

                                               aCTTCATTGGCTTGCACCGTTGGACGGTCTACCAGTTTGGTGGCACCACAACGATAATCCAGCAGATGCTGTGCCTCCTGCGGCTTCAACTGa                                         >  2:7308787/1‑93 (MQ=255)

                                                 ttCATTGGCTTGCACCGTTGGACGGTCTACCAGTTTGGTGGCGCCACAACGATAATCCAGCAGATGCTGTGCCTCCTGCGGCTTCAACTGAcg                                       >  2:2452958/1‑93 (MQ=255)

                                                 ttCATTGGCTTGCACCGTTGGACGGTCTACCAGTTTGGTGGCGCCACAACGATAATCCAGCAGATGCTGTGCCTCCTGCGGCTTCAACTGAcg                                       <  1:3412826/93‑1 (MQ=255)

                                                   cATTGGCTTGCACCGTTGGACGGTCTACCAGTTTGGTGGCGCCACAACGATAATCCAGCAGATGCTGTGCCTCCTGCGGCTTCAACTGAcggt                                     <  2:4631718/93‑1 (MQ=255)

                                                    aTTGGCTTGCACCGTTGGACGGTCTACCAGTTTGGTGGCGCCACAACGATAATCCAGCAGATGCTGTGCCTCCTGCGGCTTCAACTGAcggtc                                    <  1:7355649/93‑1 (MQ=255)

                                                    aTTGGCTTGCACCGTTGGACGGTCTACCAGTTTGGTGGCGCCACAACGATAATCCAGCAGATGCTGTGCCTCCTGCGGCTTCAACTGAcggtc                                    >  2:7378149/1‑93 (MQ=255)

                                                      tGGCTTGCACCGTTGGACGGTCTACCAGTTTGGTGGCGCCACAACGATAATCCAGCAGATGCTGTGCCTCCTGCGGCTTCAACTGAcggtcgg                                  >  1:4966802/1‑93 (MQ=255)

                                                       ggCTTGCACCGTTGGACGGTCTACCAGTTTGGTGGCGCCACAACGATAATCCAGCAGATGCTGTGCCTCCTGCGGCTTCAACTGAcggtcggt                                 <  2:929337/93‑1 (MQ=255)

                                                        gCTTGCACCGTTGGACGGTCTACCAGTTTGGTGGCGCCACAACGATAATCCAGCAGATGCTGTGCCTCCTGCGGCTTCAACTGACGGTCGGTa                                <  1:571852/93‑1 (MQ=255)

                                                        gCTTGCACCGTTGGACGGTCTACCAGTTTGGTGGCGCCACAACGATAATCCAGCAGATGCTGTGCCTCCTGCGGCTTCAACTGACGGTCGGTa                                >  1:3018090/1‑93 (MQ=255)

                                                           tGCACCGTTGGACGGTCTACCAGTTTGGTGGCGCCACAACGATAATCCAGCAGATGCTGTGCCTCCTGCGGCTTCAACTGACGGTCGGTAAAc                             <  2:4878997/93‑1 (MQ=255)

                                                           tGCACCGTTGGACGGTCTACCAGTTTGGTGGCGCCACAACGATAATCCAGCAGATGCTGTGCCTCCTGCGGCTTCAACTGACGGTAGGTAAAc                             >  2:3275789/1‑93 (MQ=255)

                                                            gCACCGTTGGACGGTCTACCAGTTTGGTGGCGCCACAACGATAATCCAGCAGATGCTGTGCCTCCTGCGGCTTCAACTGACGGTCGGTAAAcc                            <  2:4774744/93‑1 (MQ=255)

                                                             cACCGTTGGACGGTCTACCAGTTTGGTGGCGCCACAACGATAATCCAGCAGATGCTGTGCCTCCTGCGGCTTCAACTGACGGTCGGTAAAccc                           <  1:4554179/93‑1 (MQ=255)

                                                              aCCGTTGGACGGTCTACCAGTTTGGTGGCGCCACAACGATAATCCAGCAGATGCTGTGCCTCCTGCGGCTTCAACTGACGGTCGGTAAACCCg                          <  1:7607627/93‑1 (MQ=255)

                                                               ccGTTGGACGGTCTACCAGTTTGGTGGCGCCACAACGATAATCCAGCAGATGCTGTGCCTCCTGCGGCTTCAACTGACGGTCGGTAAACCCgg                         >  1:4191600/1‑93 (MQ=255)

                                                                 gTTGGACGGTCTACCAGTTTGGTGGCGCCACAACGATAATCCAGCAGATGCTGTGCCTCCTGCGGCTTCAACTGACGGTCGGTAAACCCGGcc                       >  1:1170953/1‑93 (MQ=255)

                                                                 gTTGGACGGTCTACCAGTTTGGTGGCGCCACAACGATAATCCAGCAGATGCTGTGCCTCCTGCGGCTTCAACTGACGGTCGGTAAACCCGGcc                       >  2:4102182/1‑93 (MQ=255)

                                                                 gTTGGACGGTCTACCAGTTTGGTGGCGCCACAACGATAATCCAGCAGATGCTGTGCCTCCTGCGGCTTCAACTGACGGTCGGTAAACCCGGcc                       >  1:1479255/1‑93 (MQ=255)

                                                                 gTTGGACGGTCTACCAGTTTGGTGGCGCCACAACGATAATCCAGCAGATGCTGTGCCTCCTGCGGCTTCAAATGACGGTCGGTAAACCCGGcc                       >  2:1784789/1‑93 (MQ=255)

                                                                   tGGACGGTCTACCAGTTGTGTGGCCCCACAACGATAATCCAGCAGATGCTGTGCCTCCTGCGGCTTCAACTGACGGTCGGTAAACCCGGCCTg                     <  1:5422476/93‑1 (MQ=255)

                                                                      aCGGTCTACCAGTTTGGTGGCGCCACAACGATAATCCAGCAGATGCTGTGCCTCCTGCGGCTTCAACTGACGGTCGGTAAACCCGGCCTGata                  <  1:745459/93‑1 (MQ=255)

                                                                      aCGGTCTACCAGTTTGGTGGCGCCACAACGATAATCCAGCAGATGCTGTGCCTCCTGCGGCTTCAACTGACGGTCGGTAAACCCGGCCTGata                  <  1:7767607/93‑1 (MQ=255)

                                                                         gTCTACCAGTTTGGTGGCGCCACAACGATAATCCAGCAGATGCTGTGCCTCCTGCGGCTTCAACTGACGGTCGGTAAACCCGGCCTGatatat               <  1:1331732/93‑1 (MQ=255)

                                                                         gTCTACCAGTTTGGTGGCGCCACAACGATAATCCAGCAGATGCTGTGCCTCCTGCGGCTTCAACTGACGGTCGGTAAACCCGGCCTGatatat               >  1:7896827/1‑93 (MQ=255)

                                                                         gTCTACCAGTTTGGTGGCGCCACAACGATAATCCAGCAGATGCTGTGCCTCCTGCGGCTTCAACTGACGGTCGGTAAACCCGGCCTGatatat               >  2:2053001/1‑93 (MQ=255)

                                                                          tCTACCATTTTGGTGGCGCCACAACAATAATCTAGCAGATGCAGTGCCTCCTGCGGCTTCAACTGACAGTCGGTAATCCCAGCCTGGTATATc              <  2:7004479/93‑1 (MQ=255)

                                                                          tCTACCAGTTTGGTGGCGCCACAACGATAATCCAGCAGATGCTGTGCCTCCTGCGGCTTCAACTGACGGTCGGTAAACCCGGCCTGATATATc              <  2:6194367/93‑1 (MQ=255)

                                                                            tACCAGTTTGGTGGCGCCACAACGATAATCCAGCAGATGCTGTGCCTCCTGCGGCTTCAACTGACGGTCGGTAAACCCGGCCTGATATATGAc            >  2:3436091/1‑93 (MQ=255)

                                                                            tACCAGTTTGGTGGCGCCACAACGATAATCCAGCAGATGCTGTGCCTCCTGCGGCTTCAACTGACGGTCGGTAAACCCGGCCTGATATATCAc            <  2:1140117/93‑1 (MQ=255)

                                                                                aGTTTGGTGGCGCCACAACGATAATCCAGCAGATGCTGTGCCTCCTGCGGCTTCAACTGACGGTCGGTAAACCCGGCCTGATATATCACCTTc        >  2:709445/1‑93 (MQ=255)

                                                                                aGTTTGGTGGCGCCACAACGATAATCCAGCAGATGCTGTGCCTCCTGCGGCTTCAACTGACGGTCGGTAAACCCGGCCTGATATATCACCTTc        >  2:2791823/1‑93 (MQ=255)

                                                                                 gTTTGGTGGCGCCACAACGATAATCCAGCAGATGCTGTGCCTCCTGCGGCTTCAACTGACGGTCGGTAAACCCGGCCTGATATATCACCTTcc       >  1:7299368/1‑93 (MQ=255)

                                                                                  tttGGTGGCGCCACAACGATAATCCAGCAGATGCTGTGCCTCCTGCGGCTTCAACTGACGGTCGGTAAACCCGGCCTGATATATCACCTTCCa      >  2:1375085/1‑93 (MQ=255)

                                                                                  tttGGTGGCGCCACAACGATAATCCAGCAGATGCTGTGCCTCCTGCGGCTTCAACTGACGGTCGGTAAACCCGGCCTGATATATCACCTTCCa      <  1:3349867/93‑1 (MQ=255)

                                                                                    tggtggCGCCACAACGATAATCCAGCAGATGCTGTGCCTCCTGCGGCTTCAACTGACGGTCGGTAAACCCGGCCTGATATATCACCTTCCAGa    <  2:6424023/93‑1 (MQ=255)

                                                                                    tggtggCGCCACAACGATAATCCAGCAGATGCTGTGCCTCCTGCGGCTTCAACTGACGGTCGGTAAACCCGGCCTGATATATCACCTTCCAGa    >  2:4407833/1‑93 (MQ=255)

                                                                                      gTGGCGCCACAACGATAATCCAGCAGATGCTGTGCCTCCTGCGGCTTCAACTGACGGTCGGTAAACCCGGCCTGATATATCACCTTCCAGAAg  >  1:3893601/1‑93 (MQ=255)

TTTTTTCAATCAGCTTACGCCCGCCTGCGTGTAGCTCCTGCTTTGAAACTTCATTGGCTTGCACCGTTGGACGGTCTACCAGTTTGGTGACGCCACAACGATAATCCAGCAGATGCTGTGCCTCCTGCGGCTTCAACTGACGGTCGGTAAACCCGGCCTGATATATCACCTTCCAGAAG  >  NC_000913/3212829‑3213007

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 19 ≤` `ATCG/ATCG` `< 28 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`