BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,838,055	Δ1 bp	coding (509/1458 nt)	ascF →	PTS system enzyme IIBC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,838,055	0	G	.	100.0%	808.2 / NA	178	coding (509/1458 nt)	ascF	PTS system enzyme IIBC
Reads supporting (aligned to +/- strand): ref base G (0/0); new base . (81/97); total (81/97)

Only 100 of 180 total aligned reads displayed.

CAAAAGGATCGCCGACCTTAACCATTCTGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTCG‑C‑GCATCTGCCGCGCATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCATCGCGGGTGTGCTGGTACATCCGAGTTTTATTGAACTGATGGCGAAAG  >  NC_000913/2837968‑2838141

cAAAAGGATCGCCGACCTTAACCATTCTGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTCG‑CGGCATCTGCCGC‑CATCa                                                                                   >  2:1241889/1‑93 (MQ=255)

 aaaaGGATCGCCGACCTTAACCATTCTGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTCG‑CGGCATCTGCCGC‑CATCaa                                                                                  >  1:842496/1‑93 (MQ=255)

 aaaaGGATCGCCGACCTTAACCATTCTGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTCG‑CGGCATCTGCCGC‑CATCaa                                                                                  <  1:2584720/93‑1 (MQ=255)

  aaaGGATCGCCGACCTTAACCATTCTGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTCG‑CGGCATCTGCCGC‑CATCaaa                                                                                 <  1:2491635/93‑1 (MQ=255)

  aaaGGATCGCCGACCTTAACCATTCTGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTCG‑CGGCATCTGCCGC‑CATCaaa                                                                                 <  1:3456466/93‑1 (MQ=255)

  aaaGGATCGCCGACCTTAACCATTCTGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTCG‑CGGCATCTGCCGC‑CATCaaa                                                                                 <  2:4293148/93‑1 (MQ=255)

  aaaGGATCGCCGACCTTAACCATTCTGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTCG‑CGGCATCTGCCGC‑CATCaaa                                                                                 >  2:4218856/1‑93 (MQ=255)

  aaaGGATCGCCGACCTTAACCATTCTGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTCG‑CGGCATCTGCCGC‑CATCaaa                                                                                 >  1:1819619/1‑93 (MQ=255)

   aaGGATCGCCGACCTTAACCATTCTGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTCG‑CGGCATCTGCCGC‑CATCAAAt                                                                                <  2:2915184/93‑1 (MQ=255)

   aaGGATCGCCGACCTTAACCATTCTGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTCG‑CGGCATCTGCCGC‑CATCAAAt                                                                                >  1:3637057/1‑93 (MQ=255)

    aGGATCGCCGACCTTAGCCATTCTGAATGTGATTGGTGACGGTGCTTTCTTCTACCTGCCGCTGATGGTCG‑CGGCATCTGCTGC‑CATCAAAtt                                                                               <  2:1512284/93‑1 (MQ=255)

    aGGATCGCCGACCTTAACCATTCTGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTCG‑CGGCATCTGCCGC‑CATCAAAtt                                                                               <  2:1137714/93‑1 (MQ=255)

     ggATCGCCGACCTTAACCATTCTGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTCG‑CGGCATCTGCCGC‑CATCAAAttt                                                                              >  1:3122213/1‑93 (MQ=255)

      gATCGCCGACCTTAACCATTCTGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTCG‑CGGCATCTGCCGC‑CATCAAATTTa                                                                             <  2:3091120/93‑1 (MQ=255)

          gCCGACCTTAACCATTCTGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTCG‑CGGCATCTGCCGC‑CATCAAATTTAAAAc                                                                         >  2:2450106/1‑93 (MQ=255)

           ccGACCTTAACCATTCTGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTCG‑CGGCATCTGCCGC‑CATCAAATTTAAAAcc                                                                        <  1:2370326/93‑1 (MQ=255)

             gACCTTAACCATTCTGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTCG‑CGGCATCTGCCGC‑CATCAAATTTAAAACCaa                                                                      <  2:2826740/93‑1 (MQ=255)

             gACCTTAACCATTCTGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTCG‑CGGCATCTGCCGC‑CATCAAATTTAAAACCaa                                                                      <  2:4123628/93‑1 (MQ=255)

              aCCTTAACCATTCTGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTCG‑CGGCATCTGCCGC‑CATCAAATTTAAAACCAat                                                                     >  1:3545744/1‑93 (MQ=255)

              aCCTTAACCATTCTGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTCG‑CGGCATCTGCCGC‑CATCAAATTTAAAACCAat                                                                     <  2:2378767/93‑1 (MQ=255)

               ccTTAACCATTCTGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTCG‑CGGCATCTGCCGC‑CATCAAATTTAAAACCAata                                                                    <  1:2877181/93‑1 (MQ=255)

                cTTAACCATTCTGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTCG‑CGGCATCTGCCGC‑CATCAAATTTAAAACCAatat                                                                   >  2:1622475/1‑93 (MQ=255)

                cTTAACCATTCTGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTCG‑CGGCATCTGCCGC‑CATCAAATTTAAAACCAatat                                                                   >  1:1789433/1‑93 (MQ=255)

                cTTAACCATTCTGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTCG‑CGGCATCTGCCGC‑CATCAAATTTAAAACCAatat                                                                   <  1:3332365/93‑1 (MQ=255)

                cTTAACCATGCTGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTCG‑CGGCATCTGCCGC‑CATCAAATTTAAAACCAatat                                                                   <  2:537696/93‑1 (MQ=255)

                 ttAACCATTCTGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTCG‑CGGCATCTGCCGC‑CATCAAATTTAAAACCAATATg                                                                  >  2:3856362/1‑93 (MQ=255)

                 ttAACCATTCTGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTCG‑CGGCATCTGCCGC‑CATCAAATTTAAAACCAATATg                                                                  >  2:2698512/1‑93 (MQ=255)

                 ttAACCATTCTGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTCG‑CGGCATCTGCCGC‑CATCAAATTTAAAACCAATATg                                                                  >  1:1005117/1‑93 (MQ=255)

                  tAACCATTCTGAATGTGATTGGTGACGGTTCTTTCTTCTTCCTGCCGCTGATGGTCG‑CGGCATCTGCCGC‑CATCAAATTTAAAACCAATATGt                                                                 <  1:2125734/93‑1 (MQ=255)

                  tAACCATTCTGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTCG‑CGGCATCTGCCGC‑CATCAAATTTAAAACCAATATGt                                                                 <  1:1815947/93‑1 (MQ=255)

                  tAACCATTCTGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTCG‑CGGCATCTGCCGC‑CATCAAATTTAAAACCAATATGt                                                                 <  2:2988027/93‑1 (MQ=255)

                    aCCATTCTGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTCG‑CGGCATCTGCCGC‑CATCAAATTTAAAACCAATATGTCg                                                               >  2:546835/1‑93 (MQ=255)

                     ccATTCTGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTCG‑CGGCATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGc                                                              <  1:1019393/93‑1 (MQ=255)

                      cATTCTGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTCG‑CGGCATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCt                                                             <  2:3473038/93‑1 (MQ=255)

                      cATTCTGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTCG‑CGGCATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCt                                                             <  2:2602283/93‑1 (MQ=255)

                       aTTCTGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTCG‑CGGCATCTGCCTC‑CATCAAATTTAAAACCAATATGTCGCTg                                                            <  2:2860607/93‑1 (MQ=255)

                       aTTCTGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTCG‑CGGCATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTg                                                            >  1:2760363/1‑93 (MQ=255)

                       aTTCTGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTCG‑CGGCATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTg                                                            >  2:3684090/1‑93 (MQ=255)

                       aTTCTGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTCG‑CGGCATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTg                                                            >  1:4311535/1‑93 (MQ=255)

                        ttCTGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTCG‑CGGCATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTgg                                                           >  1:1513455/1‑93 (MQ=255)

                         tCTGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTCG‑CGGCATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGc                                                          <  1:133020/93‑1 (MQ=255)

                          atGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTCG‑CGGCATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCg                                                         <  1:4163223/92‑1 (MQ=255)

                            gAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTCG‑CGGCATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGAt                                                       <  2:3453085/93‑1 (MQ=255)

                            gAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTCG‑CGGCATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGAt                                                       <  2:3219356/93‑1 (MQ=255)

                             aaTGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTCG‑CGGCATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGAtt                                                      >  2:2389018/1‑93 (MQ=255)

                               tgtgATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTCG‑CGGCATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGATTGc                                                    >  1:2176712/1‑93 (MQ=255)

                                  gATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTCG‑CGGCATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCAt                                                 >  1:2313516/1‑93 (MQ=255)

                                  gATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTCG‑CGGCATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCAt                                                 >  2:4025856/1‑93 (MQ=255)

                                  gATGGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTCG‑CGGCATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCAt                                                 <  2:4560091/93‑1 (MQ=255)

                                   aTTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTCG‑CGGCATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCATc                                                >  1:812680/1‑93 (MQ=255)

                                    ttGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTCG‑CGGCATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCATcg                                               >  2:3161102/1‑93 (MQ=255)

                                     tGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTCG‑CGGCATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCATcgc                                              <  1:4516907/93‑1 (MQ=255)

                                     tGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTCG‑CGGCATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCATcgc                                              <  1:3689712/93‑1 (MQ=255)

                                       gTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTCG‑CGGCATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCATCGCgg                                            >  1:679789/1‑93 (MQ=255)

                                        tGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTCG‑CGGCATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCATCGCggg                                           <  1:2560839/93‑1 (MQ=255)

                                         gACGGTGATTTCTTCTTCCTGCCGCTGATGGTCGAG‑GCATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCATCGCgggg                                          >  2:3334668/1‑92 (MQ=255)

                                          aCGGTGCTTTCTTCTTCCTGCCGCTGATGGTCG‑CGGCATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCATCGCGGgtg                                         <  1:3877013/93‑1 (MQ=255)

                                           cGGTGCTTTCTTCTTCCTGCCGCTGATGGTCG‑CGGCATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCATCGCGGgtgt                                        <  2:4655390/93‑1 (MQ=255)

                                            ggTGCTTTCTTCTTCCTGCCGCTGATGGTCG‑CGGCATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCATCGCGGGtgtg                                       >  1:3763880/1‑93 (MQ=255)

                                             gTGCTTTCTTCTTCCTGCCGCTGATGGTCG‑CGGCATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCATCGCGGGTGTGc                                      <  1:3612083/93‑1 (MQ=255)

                                              tGCTTTCTTCTTCCTGCCGCTGATGGTCG‑CGGCATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCATCGCGGGTGTGCt                                     >  1:1707938/1‑93 (MQ=255)

                                                cTTTCTTCTTCCTGCCGCTGATGGTCG‑CGGCATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCATCGCGGGTGTGCTgg                                   >  2:564664/1‑93 (MQ=255)

                                                cTTTCTTCTTCCTGCCGCTGATGGTCG‑CGGCATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCATCGCGGGTGTGCTgg                                   <  1:3108698/93‑1 (MQ=255)

                                                 tttCTTCTTCCTGCCGCTGATGGTCG‑CGGCATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCATCGCGGGTGTGCTGGt                                  >  2:873498/1‑93 (MQ=255)

                                                 tttCTTCTTCCTGCCGCTGATGGTCG‑CGGCATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCATCGCGGGTGTGCTGGt                                  >  1:4446425/1‑93 (MQ=255)

                                                  ttcttcttcCTGCCGCTGATGGTCG‑CGGCATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCATCGCGGGTGTGCTGGTa                                 >  2:2096874/1‑93 (MQ=255)

                                                    cttcttcCTGCCGCTGATGGTCGTG‑GCATCTGCCGC‑CATCAAATTTGAAACCAATATGTCGCTGGCGATTGCCATCGCGGGTGTGCTGGTACa                               >  2:3780919/1‑93 (MQ=255)

                                                    cttcttcCTGCCGCTGATGGTCG‑CGGCATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCATCGCGGGTGTGCTGGTACa                               <  2:231621/93‑1 (MQ=255)

                                                      tcttcCTGCCGCTGATGGTCG‑CGGCATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCATCGCGGGTGTGCTGGTACATc                             >  1:1273711/1‑93 (MQ=255)

                                                         tcCTGCCGCTGATGGTCG‑CGGCATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCATCGCGGGTGTGCTGGTACATCCGa                          <  1:4148252/93‑1 (MQ=255)

                                                          ccTGCCGCTGATGGTCG‑CGGCATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCATCGCGGGTGTGCTGGTACATCCGAg                         <  2:813186/93‑1 (MQ=255)

                                                          ccTGCCGCTGATGGTCG‑CGGCATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCATCGCGGGTGTGCTGGTACATCCGAg                         <  1:4466840/93‑1 (MQ=255)

                                                           cTGCCGCTGATGGTCG‑CGGCATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCATCGCGGGTGTGCTGGTACATCCGAGt                        >  2:3541484/1‑93 (MQ=255)

                                                           cTGCCGCTGATGGTCG‑CGGCATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCATCGCGGGTGTGCTGGTACATCCGAGt                        <  2:1692545/93‑1 (MQ=255)

                                                            cgCCGCTGATGGTCG‑CGGCATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCATCGCGGGTGTGCTGGTACATCCGAGtt                       <  1:3415773/92‑1 (MQ=255)

                                                              ccGCTGATGGTCG‑CGGCATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCATCGCGGGTGTGCTGGTACATCCGAGtttt                     <  1:1055674/93‑1 (MQ=255)

                                                               cGCTGATGGTCG‑CGGCATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCATCGCGGGTGTGCTGGTACATCCGAGTTTTa                    <  2:2075669/93‑1 (MQ=255)

                                                               cGCTGATGGTCG‑CGGCATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCATCGCGGGTGTGCTGGTACATCCGAGTTTTa                    <  2:2741044/93‑1 (MQ=255)

                                                                gCTGATGGTCG‑CGGCATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCATCGCGGGTGTGCTGGTACATCCGAGTTTTAt                   >  1:1886469/1‑93 (MQ=255)

                                                                gCTGATGGGCG‑CGGCATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCATCGCGGGTGTGCTGGTACATCCGAGTTTTAt                   <  1:2536893/93‑1 (MQ=255)

                                                                 cTGATGGTCG‑CGGCATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCATCGCGGGTGTGCTGGTACATCCGAGTTTTAtt                  >  1:1339410/1‑93 (MQ=255)

                                                                  tGATGGTCG‑CGGCATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCATCGCGGGTGTGCTGGTACATCCGAGTTTTATTg                 >  1:1267194/1‑93 (MQ=255)

                                                                  tGATGGTCG‑CGGCATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCATCGCGGGTGTGCTGGTACATCCGAGTTTTATTg                 <  1:3096164/93‑1 (MQ=255)

                                                                  tGATGGTCG‑CGGCATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCATCGCGGGTGTGCTGGTACATCCGAGTTTTATTg                 >  1:3996217/1‑93 (MQ=255)

                                                                     tGGTCG‑CGGCATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCATCGCGGGTGTGCTGGTACATCCGAGTTTTATTGAAc              >  2:210759/1‑93 (MQ=255)

                                                                     tGGTCG‑CGGCATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCATCGCGGGTGTGCTGGTACATCCGAGTTTTATTGAAc              <  1:230048/93‑1 (MQ=255)

                                                                       gTCG‑CGGCATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCATCGCGGGTGTGCTGGTACATCCGAGTTTTATTGAACTg            >  1:4385021/1‑93 (MQ=255)

                                                                       gTCG‑CGGCATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCATCGCGGGTGTGCTGGTACATCCGAGTTTTATTGAACTg            >  2:1761677/1‑93 (MQ=255)

                                                                       gTCG‑CGGCATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCATCGCGGGTGTGCTGGTACATCCGAGTTTTATTGAACTg            <  1:3203729/93‑1 (MQ=255)

                                                                           gcggcATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCATCGCGGGTGTGCTGGTACATCCGAGTTTTATTGAACTGATg         <  1:2947331/90‑1 (MQ=255)

                                                                            cggcATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCATCGCGGGTGTGCTGGTACATCCGAGTTTTATTGAACTGATgg        >  1:4055264/3‑93 (MQ=255)

                                                                             ggcATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCATCGCGGGTGTGCTGGTACATCCGAGTTTTATTGCACTGATGGc       >  2:3067898/2‑93 (MQ=255)

                                                                             ggcATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCATCGCGGGTGTGCTGGTACATCCGAGTTTTATTGAACTGATGGc       >  2:1916037/2‑93 (MQ=255)

                                                                             ggcATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCATCGCGGGTGTGCTGGTACATCCGAGTTTTATTGAACTGATGGc       <  2:1990532/92‑1 (MQ=255)

                                                                             ggcATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCATCGCGGGTGTGCTGGTACATCCGAGTTTTATTGAACTGATGGc       >  2:2266979/2‑93 (MQ=255)

                                                                              gcATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCATCGCGGGTGTGCTGGTACATCCGAGTTTTATTGAACTGATGGCg      <  2:4135504/93‑1 (MQ=255)

                                                                                 tCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCATCGCGGGTGTGCTGGTACATCCGAGTTTTATTGAACTGATGGCGaaa   <  2:3991439/93‑1 (MQ=255)

                                                                                 tCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCATCGCGGGTGTGCTGGTACATCCGAGTTTTATTGAACTGATGGCGaaa   <  1:555959/93‑1 (MQ=255)

                                                                                 tCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCATCGCGGGTGTGCTGGTACATCCGAGTTTTATTGAACTGATGGCGaaa   <  2:624668/93‑1 (MQ=255)

                                                                                 tCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCATCGCGGGTGTGCTGGTACATCCGAGTTTTATTGAACTGATGGCGaaa   >  1:739015/1‑93 (MQ=255)

CAAAAGGATCGCCGACCTTAACCATTCTGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTCG‑C‑GCATCTGCCGCGCATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCATCGCGGGTGTGCTGGTACATCCGAGTTTTATTGAACTGATGGCGAAAG  >  NC_000913/2837968‑2838141

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`