BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,974,162	(A)_6→5	coding (118/927 nt)	motB ←	MotB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,974,157	0	A	.	96.4%	478.4 / 9.5	110	coding (123/927 nt)	motB	MotB
Reads supporting (aligned to +/- strand): ref base A (2/2); new base . (58/48); total (60/50)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.06e-01

Only 100 of 123 total aligned reads displayed.

CCCGTAACCGCAGTCGCCAGTGGAGTCCGGAAGTACTCCGCAATCTGAATCAGCTCTTTTGGGCTGGAGATGGAGATCAGCCACATCACCAGAAAAAAGGCCATCATCGCAGTCATAAAGTCGGCATAAGCAATCTTCCACGATCCATGTGCTGCCCCGTGGCTTTTGGCTTTGCGTCGTTT  >  NC_000913/1974065‑1974246

cccGTAACCGCAGTCGCCAGTGGAGTCCGGAAGTACTCCGCAATCTGAATCAGCTCTTTTGGGATGGAGATGGAGATCAGCCACATCACCAGa                                                                                           >  2:2700378/1‑93 (MQ=255)

 ccGTAACCGCAGTCGCCAGCGGAGTCCGGAAGTACTCCGCAATCTGAATCAGCTCTTTTGGGCTGGAGATGGAGATCAGCCACATCACCAGaa                                                                                          >  2:4563252/1‑93 (MQ=255)

     acccGCAGTCGCCAGTGGAGTCCGGAAGTACTCCGCAATCTGAATCAGCTCTTTTGGGCTGGAGATGGAGATCAGCCACATCACCAGaaaaag                                                                                      <  2:1189714/91‑2 (MQ=255)

     aaCCGCAGTCGCCAGTGGAGTCCGGAAGTACTCCGCAATCTGAATCAGCTCTTTTGGGCTGGAGATGGAGATCAGCCACATCACCAGaaaaag                                                                                      <  2:3618474/93‑2 (MQ=255)

     aaCCGCAGTCCCCAGTGGAGTCCGGAAGTACTCCGCAATCTGAATCAGCTCTTTTGGGCTGGAGATGGAGATCAGCCACATCACCAGaaaaag                                                                                      >  2:1433210/1‑92 (MQ=255)

      aCCGCAGTCGCCAGTGGAGTCCGGAAGTACTCCGCAATCTGAATCAGCTCTTTTGGGCTGGAGATGGAGATCAGCCACATCACCAGaaaaagg                                                                                     >  2:1060483/1‑91 (MQ=255)

        cGCAGTCGCCAGTGGAGTCCGGAAGTACTCCGCAATCTGAATCAGCTCTTTTGGGCTGGAGATGGAGATCAGCCACATCACCAGaaaaaggcc                                                                                   >  1:731178/1‑89 (MQ=255)

        cGCAGTCGCCAGTGGAGTCCGGAAGTACTCCGCAATCTGAATCAGCTCTTTTGGGCTGGAGATGGAGATCAGCCACATCACCAGaaaaaggcc                                                                                   <  2:2673285/93‑5 (MQ=255)

         gCAGTCGCCAGTGGAGTCCGGAAGTACTCCGCAATCTGAATCAGCTCTTTTGGGCTGGAGATGGAGATCAGCCACATCACCAG‑AAAAAGGCca                                                                                 >  2:1128073/1‑93 (MQ=255)

         gCAGTCGCCAGTGGAGTCCGGAAGTACTCCGCAATCTGAATCAGCTCTTTTGGGCTGGAGATGGAGATCAGCCACATCACCAG‑AAAAAGGCca                                                                                 >  1:2584451/1‑93 (MQ=255)

            gTCGCCAGTGGAGTCCGGAAGTACTCCGCAATCTGAATCAGCTCTTTTGGGCTGGAGATGGAGATCAGCCACATCACCAG‑AAAAAGGCcatca                                                                              <  1:1450758/93‑1 (MQ=255)

            gTCGCCAGTGGAGTCCGGAAGTACTCCGCAATCTGAATCAGCTCTTTTGGGCTGGAGATGGAGATCAGCCACATCACCAG‑AAAAAGGCcatca                                                                              >  2:930324/1‑93 (MQ=255)

              cGCCAGTGGAGTCCGGAAGTACTCCGCAATCTGAATCAGCTCTTTTGGGCTGGAGATGGAGATCAGCCACATCACCAG‑AAAAAGGCCatcatc                                                                            <  1:1874560/93‑1 (MQ=255)

              cGCCAGTGGAGTCCGGAAGTACTCCGCAATCTGAATCAGCTCTTTTGGGCTGGAGATGGAGATCAGCCACATCACCAG‑AAAAAGGCCatcatc                                                                            <  2:4388071/93‑1 (MQ=255)

              cGCCAGTGGAGTCCGGAAGTACTCCGCAATCTGAATCAGCTCTTTTGGGCTGGAGATGGAGATCAGCCACATCACCAG‑AAAAAGGCCatcatc                                                                            >  2:1172735/1‑93 (MQ=255)

               gCCAGTGGAGTCCGGAAGTACTCCGCAATCTGAATCAGCTCTTTTGGGCTGGAGATGGAGATCAGCCACATCACCAG‑AAAAAGGCCATCATCg                                                                           >  1:1379744/1‑93 (MQ=255)

                ccAGTGGAGTCCGGAAGTACTCCGCAATCTGAATCAGCTCTTTTGGGCTGGAGATGGAGATCAGCCACATCACCAG‑AAAAAGGCCATCATCGc                                                                          >  2:4004569/1‑93 (MQ=255)

                ccAGTGGAGTCCGGAAGTACTCCGCAATCTGAATCAGCTCTTTTGGGCTGGAGATGGAGATCAGCCACATCACCAG‑AAAAAGGCCATCATCGc                                                                          >  2:1804018/1‑93 (MQ=255)

                 cAGTGGAGTCCGGAAGTACTCCGCAATCTGAATCAGCTCTTTTGGGCTGGAGATGGAGATCAGCCACATCACCAG‑AAAAAGGCCATCATCGCa                                                                         >  2:1220582/1‑93 (MQ=255)

                  aGTGGAGTCCGGAAGTACTCCGCAATCTGAATCAGCTCTTTTGGGCTGGAGATGGAGATCAGCCACATCACCAG‑AAAAAGGCCATCATCGCAg                                                                        <  1:2853123/93‑1 (MQ=255)

                  aGTGGAGTCCGGAAGTACTCCGCAATCTGAATCAGCTCTTTTGGGCTGGAGATGGAGATCAGCCACATCACCAG‑AAAAAGGCCATCATCGCAg                                                                        >  2:1313811/1‑93 (MQ=255)

                    tGGAGTCCGGAAGTACTCCGCAATCTGAATCAGCTCTTTTGGGCTGGAGATGGAGATCAGCCACATCACCAG‑AAAAAGGCCATCATCGCAGTc                                                                      >  1:3738391/1‑93 (MQ=255)

                     ggAGTCCGGAAGTACTCCGCAATCTGAATCAGCTCTTTTGGGCTGGAGATGGAGATCAGCCACATCACCAG‑AAAAAGGCCATCATCGCAGTCa                                                                     >  1:4070015/1‑93 (MQ=255)

                      gAGTCCGGAAGTACTCCGCAATCTGAATCAGCTCTTTTGGGCTGGAGATGGAGATCAGCCACATCACCAG‑AAAAAGGCCATCATCGCAGTCAt                                                                    <  2:3134154/93‑1 (MQ=255)

                      gAGTCCGGAAGTACTCCGCAATCTGAATCAGCTCTTTTGGGCTGGAGATGGAGATCAGCCACATCACCAG‑AAAAAGGCCATCATCGCAGTCAt                                                                    >  1:239700/1‑93 (MQ=255)

                       aGTCCGGAAGTACTCCGCAATCTGAATCAGCTCTTTTGGGCTGGAGATGGAGATCAGCCACATCACCAG‑AAAAAGGCCATCATCGCAGTCATa                                                                   >  1:1663345/1‑93 (MQ=255)

                        gTCCGGAAGTACTCCGCAATCTGAATCAGCTCTTTTGGGCTGGAGATGGAGATCAGCCACATCACCAG‑AAAAAGGCCATCATCGCAGTCATaa                                                                  >  2:592304/1‑93 (MQ=255)

                        gTCCGGAAGTACTCCGCAATCTGAATCAGCTCTTTTGGGCTGGAGATGGAGATCAGCCACATCACCAG‑AAAAAGGCCATCATCGCAGTCATaa                                                                  >  2:2001390/1‑93 (MQ=255)

                        gTCCGGAAGTACTCCGCAATCTGAATCAGCTCTTTTGGGCTGGAGATGGAGATCAGCCACATCACCAG‑AAAAAGGCCATCATCGCAGTCATaa                                                                  <  1:523164/93‑1 (MQ=255)

                         tCCGGAAGTACTCCGCAATCTGAATCAGCTCTTTTGGGCTGGAGATGGAGGTCAGCCACATCACCAG‑AAAAAGGCCATCATCGCAGTCATaaa                                                                 <  1:2516825/93‑1 (MQ=255)

                         tCCGGAAGTACTCCGCAATCTGAATCAGCTCTTTTGGGCTGGAGATGGAGATCAGCCACATCACCAG‑AAAAAGGCCATCATCGCAGTCATaaa                                                                 >  1:1740132/1‑93 (MQ=255)

                         gccGGAAGTACTCCGCAATCTGAATCAGCTCTTTTGGGCTGGAGATGGAGATCAGCCACATCACCAG‑AAAAAGGCCATCATCGCAGTCATaaa                                                                 <  2:3404913/92‑1 (MQ=255)

                           cGGAAGTACTCCGCAATCTGAATCAGCTCTTTTGGGCTGGAGATGGAGATCAGCCACATCACCAG‑AAAAAGGCCATCATCGCAGTCATAAAGt                                                               <  2:1344360/93‑1 (MQ=255)

                             gAAGTACTCCGCAATCTGAATCAGCTCTTTTGGGCTGGAGATGGAGATCAGCCACATCACCAG‑AAAAAGGCCATCATCGCAGTCATAAAGTCg                                                             >  2:3314868/1‑93 (MQ=255)

                                   cTCCGCAATCTGAATCAGCTCTTTTGGGCTGGAGATGGAGATCAGCCACATCACCAG‑AAAAAGGCCATCATCGCAGTCATAAAGTCGGCATaa                                                       >  2:2588887/1‑93 (MQ=255)

                                   cTCCGCAATCTGAATCAGCTCTTTTGGGCTGGAGATGGAGATCAGCCACATCACCAG‑AAAAAGGCCATCATCGCAGTCATAAAGTCGGCATaa                                                       <  2:3447485/93‑1 (MQ=255)

                                   cTCCGCAATCTGAATCAGCTCTTTTGGGCTGGAGATGGAGATCAGCCACATCACCAG‑AAAAAGGCCATCATCGCAGTCATAAAGTCGGCATaa                                                       >  2:3092652/1‑93 (MQ=255)

                                    tCCGCAATCTGAATCAGCTCTTTTGGGCTGGAGATGGAGATCAGCCACATCACCAG‑AAAAAGGCCATCATCGCAGTCATAAAGTCGGCATAAg                                                      >  1:4357218/1‑93 (MQ=255)

                                       gCAATCTGAATCAGCTCTTTTGGGCTGGAGATGGAGATCAGCCACATCACCAG‑AAAAAGGCCATCATCGCAGTCATAAAGTCGGCATAAGCaa                                                   >  2:73051/1‑93 (MQ=255)

                                       gCAATCTGAATCAGCTCTTTTGGGCTGGAGATGGAGATCAGCCACATCACCAG‑AAAAAGGCCATCATCGCAGTCATAAAGTCGGCATAAGCaa                                                   <  1:3737076/93‑1 (MQ=255)

                                        cAATCTGAATCAGCTCTTTTGGGCTGGAGATGGAGATCAGCCACATCACCAG‑AAAAAGGCCATCATCGCAGTCATAAAGTCGGCATAAGCAAt                                                  <  1:1022223/93‑1 (MQ=255)

                                        cAATCTGAATCAGCTCTTTTGGGCTGGAGATGGAGATCAGCCACATCACCAG‑AAAAAGGCCATCATCGCAGTCATAAAGTCGGCATAAGCAAt                                                  <  2:4182493/93‑1 (MQ=255)

                                        cAATCTGAATCAGCTCTTTTGGGCTGGAGATGGAGATCAGCCACATCACCAG‑AAAAAGGCCATCATCGCAGTCATAAAGTCGGCATAAGCAAt                                                  <  2:3629663/93‑1 (MQ=255)

                                           tCTGAATCAGCTCTTTTGGGCTGGAGATGGAGATCAGCCACATCACCAG‑AAAAAGGCCATCATCGCAGTCATAAAGTCGGCATAAGCAATCtt                                               >  2:3384353/1‑93 (MQ=255)

                                           tCTGAATCAGCTCTTTTGGGCTGGAGATGGAGATCAGCCACATCACCAG‑AAAAAGGCCATCATCGCAGTCATAAAGTCGGCATAAGCAATCtt                                               <  1:2672186/93‑1 (MQ=255)

                                            cTGACTCAGCTCTTTTGGGCTGGAGATGGAGATCAGCCACATCACCAG‑AAAAAGGCCATCATCGCAGTCATAAAGTCGGCATAAGCAATCTTc                                              <  2:1369760/93‑1 (MQ=255)

                                            cTGAATCAGCTCTTTTGGGCTGGAGATGGAGATCAGCCACATCACCAG‑AAAAAGGCCATCATCGCAGTCATAAAGTCGGCATAAGCAATCTTc                                              <  1:4513223/93‑1 (MQ=255)

                                             tGAATCAGCTCTTTTGGGCTGGAGATGGAGATCAGCCACATCACCAG‑AAAAAGGCCATCATCGCAGTCATAAAGTCGGCATAAGCAATCTTcc                                             >  1:223071/1‑93 (MQ=255)

                                              gAATCAGCTCTTTTGGGCTGGAGATGGAGATCAGCCACATCACCAG‑AAAAAGGCCATCATCGCAGTCATAAAGTCGGCATAAGCAATCTTCCa                                            <  1:647202/93‑1 (MQ=255)

                                              gAATCAGCTCTTTTGGGCTGGAGATGGAGATCAGCCACATCACCAG‑AAAAAGGCCATCATCGCAGTCATAAAGTCGGCATAAGCAATCTTCCa                                            <  2:1457522/93‑1 (MQ=255)

                                               aaTCAGCTCTTTTGGGCTGGAGATGGAGATCAGCCACATCACCAG‑AAAAAGGCCATCATCGCAGTCATAAAGTCGGCATAAGCAATCTTCCAc                                           >  1:2336502/1‑93 (MQ=255)

                                               aaTCAGCTCTTTTGGGCTGGAGATGGAGATCAGCCACATCACCAG‑AAAAAGGCCATCATCGCAGTCATAAAGTCGGCATAAGCAATCTTCCAc                                           >  2:539622/1‑93 (MQ=255)

                                               aaTCAGCTCTTTTGGGCTGGAGATGGAGATCAGCCACATCACCAG‑AAAAAGGCCATCATCGCAGTCATAAAGTCGGCATAAGCAATCTTCCAc                                           <  2:3746232/93‑1 (MQ=255)

                                               aaTCAGCTCTTTTGGGCTGGAGATGGAGATCAGCCACATCACCAG‑AAAAAGGCCATCATCGCAGTCATAAAGTCGGCATAAGCAATCTTCCAc                                           <  2:759429/93‑1 (MQ=255)

                                                 tCAGCTCTTTTGGGCTGGAGATGGAGATCAGCCACATCACCAG‑AAAAAGGCCATCATCGCAGTCATAAAGTCGGCATAAGCAATCTTCCACGa                                         >  1:4229196/1‑93 (MQ=255)

                                                  cAGCTCTTTTGGGCTGGAGATGGAGATCAGCCACATCACCAG‑AAAAAGGCCATCATCGCAGTCATAAAGTCGGCATAAGCAATCTTCCACGAt                                        <  2:679444/93‑1 (MQ=255)

                                                   aGCTCTTTTGGGCTGGAGATGGAGATCAGCCACATCACCAGAAAAAAGGCCATCATCGCAGTCATAAAGTCGGCATAAGCAATCTTCCACGAt                                        <  2:4050306/93‑1 (MQ=255)

                                                    gCTCTTTTGGGCTGGAGATGGAGATCAGCCACATCACCAG‑AAAAAGGCCATCATCGCAGTCATAAAGTCGGCATAAGCAATCTTCCACGATcc                                      >  2:1427111/1‑93 (MQ=255)

                                                    gCTCTTTTGGGCTGGAGATGGAGATCAGCCACATCACCAG‑AAAAAGGCCATCATCGCAGTCATAAAGTCGGCATAAGCAATCTTCCACGATcc                                      >  1:4470364/1‑93 (MQ=255)

                                                     ctctTTTGGGCTGGAGATGGAGATCAGCCACATCACCAG‑AAAAAGGCCATCATCGCAGTCATAAAGTCGGCATAAGCAATCTTCCACGATCCa                                     <  2:4340197/93‑1 (MQ=255)

                                                      tctTTTGGGCTGGAGATGGAGATCAGCCACATCACCAG‑AAAAAGGCCATCATCGCAGTCATAAAGTCGGCATAAGCAATCTTCCACGATCCAt                                    >  2:1028437/1‑93 (MQ=255)

                                                      tctTTTGGGCTGGAGATGGAGATCAGCCACATCACCAG‑AAAAAGGCCATCATCGCAGTCATAAAGTCGGCATAAGCAATCTTCCACGATCCAt                                    >  1:149161/1‑93 (MQ=255)

                                                       ctTTTGGGCTGGAGATGGAGATCAGCCACATCACCAG‑AAAAAGGCCATCATCGCAGTCATAAAGTCGGCATAAGCAATCTTCCACGATCCAtg                                   <  2:56303/93‑1 (MQ=255)

                                                       ctTTAGGGCTGAAGATGGAGATCAGCCACATCACCAG‑AAAAAGGCCATCATCGCAGTCATAAAGTCGGCATAAGCAATCTTCCACGATCCAtg                                   <  1:4424909/93‑1 (MQ=255)

                                                        ttttGGGCTGGAGATGGAGATCAGCCACATCACCAG‑AAAAAGGCCATCATCGCAGTCATAAAGTCGGCATAAGCAATCTTCCACGATCCAtgt                                  >  1:3628264/1‑93 (MQ=255)

                                                        ttttGGGCTGGAGATGGAGATCAGCCACATCACCAG‑AAAAAGGCCATCATCGCAGTCATAAAGTCGGCATAAGCAATCTTCCACGATCCAtgt                                  <  1:20786/93‑1 (MQ=255)

                                                         tttGGGCTGGAGATGGAGATCAGCCACATCACCAG‑AAAAAGGCCATCATCGCAGTCATAAAGTCGGCATAAGCAATCTTCCACGATCCAtgtg                                 >  1:2054406/1‑93 (MQ=255)

                                                          ttGGGCTGGAGATGGAGATCAGCCACATCACCAG‑AAAAAGGCCATCATCGCAGTCATAAAGTCGGCATAAGCAATCTTCCACGATCCATGtgc                                <  1:4191061/93‑1 (MQ=255)

                                                           tGGGCTGGAGATGGAGATCAGCCACATCACCAG‑AAAAAGGCCATCATCGCAGTCATAAAGTCGGCATAAGCAATCTTCCACGATCCATGtgct                               <  1:1592734/93‑1 (MQ=255)

                                                              gCTGGAGATGGAGATCAGCCACATCACCAG‑AAAAAGGCCATCATCGCAGTCATAAAGTCGGCATAAGCAATCTTCCACGATCCATGTGCTGcc                            <  1:3119347/93‑1 (MQ=255)

                                                              gCTGGAGATGGAGATCAGCCACATCACCAG‑AAAAAGGCCATCATCGCAGTCATAAAGTCGGCATAAGCAATCTTCCACGATCCATGTGCTGcc                            <  1:4066175/93‑1 (MQ=255)

                                                                    gatggagatCAGCCACATCACCAG‑AAAAAGGCCATCATCGCAGTCATAAAGTCGGCATAAGCAATCTTCCACGATCCATGTGCTGCCCCGtgg                      >  1:2801397/1‑93 (MQ=255)

                                                                      tggagatCAGCCACATCACCAG‑AAAAAGGCCATCATCGCAGTCATAAAGTCGGCATAAGCAATCTTCCACGATCCATGTGCTGCCCCGtggct                    <  1:4030082/93‑1 (MQ=255)

                                                                      tggagatCAGCCACATCACCAG‑AAAAAGGCCATCATCGCAGTCATAAAGTCGGCATAAGCAATCTTCCACGATCCATGTGCTGCCCCGtggct                    >  2:3347496/1‑93 (MQ=255)

                                                                       ggagatCAGCCACATCACCAG‑AAAAAGGCCATCATCGCAGTCATAAAGTCGGCATAAGCAATCTTCCACGATCCATGTGCTGCCCCGtggctt                   >  2:2950589/1‑93 (MQ=255)

                                                                         gaatCAACCACATCACCAG‑AAAAAGGCCATCATCGCAGTCATAAAGTCGGCATAAGCAATCTTCCACGATCCATGTGCTGCCCCGtggctttt                 <  1:1270016/91‑1 (MQ=255)

                                                                         agatCAGCCACATCACCAG‑AAAAAGGCCATCATCGCAGTCATAAAGTCGGCATAAGCAATCTTCCACGATCCATGTGCTGCCCCGtggctttt                 <  2:222147/93‑1 (MQ=255)

                                                                         agatCAGCCACATCACCAG‑AAAAAGGCCATCATCGCAGTCATAAAGTCGGCATAAGCAATCTTCCACGATCCATGTGCTGCCCCGtggctttt                 <  2:1905890/93‑1 (MQ=255)

                                                                         agagCAGCCACATCACC‑GGAAAAAGGCCATCATCGCAGTCATAAAGTCGGCATAAGCAATCTTCCACGATCCATGTGCTGCCCCGtggctttt                 <  1:3603691/93‑1 (MQ=255)

                                                                          gatCAGCCACATCACCAG‑AAAAAGGCCATCATCGCAGTCATAAAGTCGGCATAAGCAATCTTCCACGATCCATGTGCTGCCCCGtggcttttg                <  2:2511939/93‑1 (MQ=255)

                                                                          gatCAGCCACATCACCAG‑AAAAAGGCCATCATCGCAGTCATAAAGTCGGCATAAGCAATCTTCCACGATCCATGTGCTGCCCCGtggcttttg                <  2:4654568/93‑1 (MQ=255)

                                                                           atCAGCCACATCACCAG‑AAAAAGGCCATCATCGCAGTCATAAAGTCGGCATAAGCAATCTTCCACGATCCATGTGCTGCCCCGtggcttttgg               >  2:255034/1‑93 (MQ=255)

                                                                           atCAGCCACATCACCAG‑AAAAAGGCCATCATCGCAGTCATAAAGTCGGCATAAGCAATCTTCCACGATCCATGTGCTGCCCCGtggcttttgg               >  2:1047904/1‑93 (MQ=255)

                                                                              aGCCACATCACCAG‑AAAAAGGCCATCATCGCAGTCATAAAGTCGGCATAAGCAATCTTCCACGATCCATGTGCTGCCCCGtggcttttggctt            >  2:549282/1‑93 (MQ=255)

                                                                               gCCACATCACCAG‑AAAAAGGCCATCATCGCAGTCATAAAGTCGGCATAAGCAATCTTCCACGATCCATGTGCTGCCCCGtggcttttggcttt           <  2:1649765/93‑1 (MQ=255)

                                                                               gCCACATCACCAG‑AAAAAGGCCATCATCGCAGTCATAAAGTCGGCATAAGCAATCTTCCACGATCCATGTGCTGCCCCGtggcttttggcttt           >  2:1626286/1‑93 (MQ=255)

                                                                               gCCACATCACCAG‑AAAAAGGCCATCATCGCAGTCATAAAGTCGGCATAAGCAATCTTCCACGATCCATGTGCTGCCCCGtggcttttggcttt           >  1:841962/1‑93 (MQ=255)

                                                                               gCCACATCACCAG‑AAAAAGGCCATCATCGCAGTCATAAAGTCGGCATAAGCAATCTTCCACGATCCATGTGCTGCCCCGtggcttttggcttt           <  1:682438/93‑1 (MQ=255)

                                                                                ccACATCACCAG‑AAAAAGGCCATCATCGCAGTCATAAAGTCGGCATAAGCAATCTTCCACGATCCATGTGCTGCCCCGTGGCTTTTGGCTTTg          >  1:4218920/1‑93 (MQ=255)

                                                                                ccACATCACCAG‑AAAAAGGCCATCATCGCAGTCATAAAGTCGGCATAAGCAATCTTCCACGATCCATGTGCTGCCCCGTGGCTTTTGGCTTTg          >  1:2018734/1‑93 (MQ=255)

                                                                                  acaTCACCAG‑AAAAAGGCCATCATCGCAGTCATAAAGTCGGCATAAGCAATCTTCCACGATCCATGTGCTGCCCCGTGGCTTTTGGCTTTGcg        >  2:4153881/1‑93 (MQ=255)

                                                                                  acaTCACCAG‑AAAAAGGCCATCATCGCAGTCATAAAGTCGGCATAAGCAATCTTCCACGATCCATGTGCTGCCCCGTGGCTTTTGGCTTTGcg        >  2:207541/1‑93 (MQ=255)

                                                                                   caTCACCAGAAAAAAGGCCATCATCGCAGTCATAAAGTCGGCATAAGCAATCTTCCACGATCCATGTGCTGCCCCGTGGCTTTTGGCTTTGcg        <  1:3640465/93‑1 (MQ=255)

                                                                                    aTCACCAG‑AAAAAGGCCATCATCGCAGTCATAAAGTCGGCATAAGCAATCTTCCACGATCCATGTGCTGCCCCGTGGCTTTTGGCTTTGcgtc      >  2:4438841/1‑93 (MQ=255)

                                                                                     tCACCAGAAAAAAGGCCATCATCGCAGTCATAAAGTCGGCATAAGCAATCTTCCACGATCCATGTGCTGCCCCGTGGCTTTTGGCTTTGcgtc      >  2:2158068/1‑93 (MQ=255)

                                                                                     tCACCAG‑AAAAAGGCCATCATCGCAGTCATAAAGTCGGCATAAGCAATCTTCCACGATCCATGTGCTGCCCCGTGGCTTTTGGCTTTGcgtcg     >  2:1728405/1‑93 (MQ=255)

                                                                                     tCACCAG‑AAAAAGGCCATCATCGCAGTCATAAAGTCGGCATAAGCAATCTTCCACGATCCATGTGCTGCCCCGTGGCTTTTGGCTTTGcgtcg     >  1:4103029/1‑93 (MQ=255)

                                                                                      cACCAG‑AAAAAGGCCATCATCGCAGTCATAAAGTCGGCATAAGCAATCTTCCACGATCCATGTGCTGCCCCGTGGCTTTTGGCTTTGcgtcgt    <  1:2986185/93‑1 (MQ=255)

                                                                                       aCCAG‑AAAAAGGCCATCATCGCAGTCATAAAGTCGGCATAAGCAATCTTCCACGATCCATGTGCTGCCCCGTGGCTTTTGGCTTTGCGTCGtt   <  2:3914869/93‑1 (MQ=255)

                                                                                         cAGAAAAAAGGCCATCATCGCAGTCATAAAGTCGGCATAAGCAATCTTCCACGATCCATGTGCTGCCCCGTGGCTTTTGGCTTTGCGTCGttt  >  1:513423/1‑93 (MQ=255)

CCCGTAACCGCAGTCGCCAGTGGAGTCCGGAAGTACTCCGCAATCTGAATCAGCTCTTTTGGGCTGGAGATGGAGATCAGCCACATCACCAGAAAAAAGGCCATCATCGCAGTCATAAAGTCGGCATAAGCAATCTTCCACGATCCATGTGCTGCCCCGTGGCTTTTGGCTTTGCGTCGTTT  >  NC_000913/1974065‑1974246

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 23 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`