BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,812,859	Δ1 bp	intergenic (‑105/+46)	ygaG ← / ← gshA	hypothetical protein/gamma‑glutamate‑cysteine ligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,812,858	0	C	.	100.0%	510.7 / NA	112	intergenic (‑104/+47)	ygaG/gshA	hypothetical protein/gamma‑glutamate‑cysteine ligase
Reads supporting (aligned to +/- strand): ref base C (0/0); new base . (75/37); total (75/37)

Only 100 of 125 total aligned reads displayed.

CGGTAATTTTTTTAAAAATTTTCTGAACTCTTTCTTCCCAGGCGAGTCTGAGTATATGAAAGACGCGCATTTGTTATCATCATCCCTGAATTCCAGAGATGAAATTTTGGCCACTCACGAGTGGCCTTTTTCTTTTCTGTCAGGCGTGTTTTTCCAGCCACACCGCAAACGGTTCGGTATC  >  NC_000913/2812766‑2812946

cGGTAATTTTTTTAAAAATTTTCTGAACTCTTTCTTCCCAGGCGAGTCTGAGTATATGAAAGACGCGCATTTGTTATCATCACCCCTGAATTc                                                                                          <  1:422996/93‑1 (MQ=255)

 ggTAATTTTTTTAAAAATTTTCTGAACTCTTTCTTCCCAGGCGAGTCTGAGTATATGAAAGACGCGCATTTGTTATCATCACCCCTGAATTca                                                                                         <  1:194789/93‑2 (MQ=255)

 ggTAATTTTTTTAAAAATTTTCTGAACTCTTTCTTCCCAGGCGAGTCTGAGTATATGAAAGACGCGCATTTGTTATCATCACCCCTGAATTca                                                                                         <  2:1904107/93‑2 (MQ=255)

 ggTAATTTTTTTAAAAATTTTCTGAACTCTTTCTTCCCAGGCGAGTCTGAGTATATGAAAGACGCGCATTTGTTATCATCACCCCTGAATTca                                                                                         >  1:2363640/1‑92 (MQ=255)

  gTAATTTTTTTAAAAATTTTCTGAACTCTTTCTTCCCAGGCGAGTCTGAGTATATGAAAGACGCGCATTTGTTATCATCACCCCTGAATTcag                                                                                        >  1:4591914/1‑91 (MQ=255)

  gTAATTTTTTTAAAAATTTTCTGAACTCTTTCTTCCCAGGCGAGTCTGAGTATATGAAAGACGCGCATTTGTTATCATCACCCCTGAATTcag                                                                                        >  1:180929/1‑91 (MQ=255)

  gTAATTTTTTTAAAAATTTTCTGAACTCTTTCTTCCCAGGCGAGTCTGAGTATATGAAAGACGCGCATTTGTTATCATCACCCCTGAATTcag                                                                                        >  2:580769/1‑91 (MQ=255)

  gTAATTTTTTTAAAAATTTTCTGAACTCTTTCTTCCCAGGCGAGTCTGAGTATATGAAAGACGCGCATTTGTTATCATCACCCCTGAATTcag                                                                                        >  2:3019251/1‑91 (MQ=255)

   tAATTTTTTTAAAAATTTTCTGAACTCTTTCTTCCCAGGCGAGTCTGAGTATATGAAAGACGCGCATTTGTTATCATCACCCCTGAATTcaga                                                                                       <  1:3410483/93‑4 (MQ=255)

    aaTTTTTTTAAAAATTTTCTGAACTCTTTCTTCCCAGGCGAGTCTGAGTATATGAAAGACGCGCATTTGTTATCATCACCCCTGAATTcagag                                                                                      <  2:2273054/93‑5 (MQ=255)

     aTTTTTTTAAAAATTTTCTGAACTCTTTCTTCCCAGGCGAGTCTGAGTATATGAAAGACGCGCATTTGTTATCATCACCCCTGAATT‑CAgaga                                                                                    >  2:1288271/1‑93 (MQ=255)

     aTTTTTTTAAAAATTTTCTGAACTCTTTCTTCCCAGGCGAGTCTGAGTATATGAAAGACGCGCATTTGTTATCATCACCCCTGAATT‑CAgaga                                                                                    >  2:423503/1‑93 (MQ=255)

      tttttttAAAAATTTTCTGAACTCTTTCTTCCCAGGCGAGTCTGAGTATATGAAAGACGCGCATTTGTTATCATCACCCCTGAATT‑CAGAGAt                                                                                   >  1:272960/1‑93 (MQ=255)

      tttttttAAAAATTTTCTGAACTCTTTCTTCCCAGGCGAGTCTGAGTATATGAAAGACGCGCATTTGTTATCATCACCCCTGAATT‑CAGAGAt                                                                                   >  2:1457204/1‑93 (MQ=255)

       ttttttAAAAATTTTCTGAACTCTTTCTTCCCAGGCGAGTCTGAGTATATGAAAGACGCGCATTTGTTATCATCACCCCTGAATT‑CAGAGATg                                                                                  <  2:86857/93‑1 (MQ=255)

       ttttttAAAAATTTTCTGAACTCTTTCTTCCCAGGCGAGTCTGAGTATATGAAAGACGCGCATTTGTTATCATCACCCCTGAATT‑CAGAGATg                                                                                  >  2:819841/1‑93 (MQ=255)

       ttttttAAAAATTTTCTGAACTCTTTCTTCCCAGGCGAGTCTGAGTATATGAAAGACGCGCATTTGTTATCATCACCCCTGAATT‑CAGAGATg                                                                                  <  2:3724447/93‑1 (MQ=255)

       ttttttAAAAATTTTCTGAACTCTTTCTTCCCAGGCGAGTCTGAGTATATGAAAGACGCGCATTTGTTATCATCACCCCTGAATT‑CAGAGATg                                                                                  >  1:223039/1‑93 (MQ=255)

        tttttAAAAATTTTCTGAACTCTTTCTTCCCAGGCGAGTCTGAGTATATGAAAGACGCGCATTTGTTATCATCACCCCTGAATT‑CAGAGATGa                                                                                 >  1:188871/1‑93 (MQ=255)

          tttAAAAATTTTCTGAACTCTTTCTTCCCAGGCGAGTCTGAGTATATGAAAGACGCGCATTTGTTATCNTCACCCCTGAATT‑CANAGATGaaa                                                                               >  2:7/1‑93 (MQ=255)

          tttAAAAATTTTCTGAACTCTTTCTTCCCAGGCGAGTCTGAGTATATGAAAGACGCGCATTTGTTATCATCACCCCTGAATT‑CAGAGATGaaa                                                                               >  1:1518560/1‑93 (MQ=255)

          tttAAAAATTTTCTGAACTCTTTCTTCCCAGGCGAGTCTGAGTATATGAAAGACGCGCATTTGTTATCATCACCCCTGAATT‑CAGAGATGaaa                                                                               >  2:435921/1‑93 (MQ=255)

          tttAAAAATTTTCTGAACTCTTTCTTCCCAGGCGAGTCTGAGTATATGAAAGACGCGCATTTGTTATCATCACCCCTGAATT‑CAGAGATGaaa                                                                               <  1:4628372/93‑1 (MQ=255)

           ttAAAAATTTTCTGAACTCTTTCTTCCCAGGCGAGTCTGAGTATATGAAAGACGCGCATTTGTTATCATCACCCCTGAATT‑CAGAGATGAAAt                                                                              >  1:2732279/1‑93 (MQ=255)

               aaaTTTTCTGAACTCTTTCTTCCCAGGCGAGTCTGAGTATATGAAAGACGCGCATTTGTTATCATCACCCCTGAATT‑CAGAGATGAAATTTTg                                                                          >  2:3226616/1‑93 (MQ=255)

               aaaTTTTCTGAACTCTTTCTTCCCAGGCGAGTCTGAGTATATGAAAGACGCGCATTTGTTATCATCACCCCTGAATT‑CAGAGATGAAATTTTg                                                                          >  2:3359489/1‑93 (MQ=255)

               aaaTTTTCTGAACTCTTTCTTCCCAGGCGAGTCTGAGTATATGAAAGACGCGCATTTGTTATCATCACCCCTGAATT‑CAGAGATGAAATTTTg                                                                          >  2:852833/1‑93 (MQ=255)

                aaTTTTCTGAACTCTTTCTTCCCAGGCGAGTCTGAGTATATGAAAGACGCGCATTTGTTATCATCACCCCTGAATT‑CAGAGATGAAATTTTgg                                                                         >  2:3834805/1‑93 (MQ=255)

                 aTTTTCTGAACTCTTTCTTCCCAGGCGAGTCTGAGTATATGAAAGACGCGCATTTGTTATCATCACCCCTGAATT‑CAGAGATGAAATTTTGGc                                                                        >  2:491940/1‑93 (MQ=255)

                    ttCTGAACTCTTTCTTCCCAGGCGAGTCTGAGTATATGAAAGACGCGCATTTGTTATCATCACCCCTGAATT‑CAGAGATGAAATTTTGGCCAc                                                                     >  1:1671518/1‑93 (MQ=255)

                       tGAACTCTTTCTTCCCAGGCGAGTCTGAGTATATGAAAGACGCGCATTTGTTATCATCACCCCTGAATT‑CAGAGATGAAATTTTGGCCACTCa                                                                  >  1:2759780/1‑93 (MQ=255)

                          aCTCTTTCTTCCCAGGCGAGTCTGAGTATATGAAAGACGCGCATTTGTTATCATCACCCCTGAATT‑CAGAGATGAAATTTTGGCCACTCACGa                                                               >  1:1561688/1‑93 (MQ=255)

                           ctctTTCTTCCCAGGCGAGTCTGAGTATATGAAAGACGCGCATTTGTTATCATCACCCCTGAATT‑CAGAGATGAAATTTTGGCCACTCACGAg                                                              <  2:886692/93‑1 (MQ=255)

                           ctctTTCTTCCCAGGCGAGTCTGAGTATATGAAAGACGCGCATTTGTTATCATCACCCCTGAATT‑CAGAGATGAAATTTTGGCCACTCACGAg                                                              >  1:4445204/1‑93 (MQ=255)

                            tctttcttCCCAGGCGAGTCTGAGTATATGAAAGACGCGCATTTGTTATCATCACCCCTGAATT‑CAGAGATGAAATTTTGGCCACTCACGAgg                                                             >  2:3478632/1‑92 (MQ=255)

                            tctttcttCCCAGGCGAGTCTGAGTATATGAAAGACGCGCATTTGTTATCATCACCCCTGAATT‑CAGAGATGAAATTTTGGCCACTCACGAgg                                                             >  2:2648559/1‑92 (MQ=255)

                            tctttcttCCCAGGCGAGTCTGAGTATATGAAAGACGCGCATTTGTTATCATCACCCCTGAATT‑CAGAGATGAAATTTTGGCCACTCACGAGt                                                             <  1:3536609/93‑1 (MQ=255)

                            tctttcttCCCAGGCGAGTCTGAGTATATGAAAGACGCGCATTTGTTATCATCACCCCTGAATT‑CAGAGATGAAATTTTGGCCACTCACGAGt                                                             >  2:1788544/1‑93 (MQ=255)

                             ctttcttCCCAGGCGAGTCTGAGTATATGAAAGACGCGCATTTGTTATCATCACCCCTGAATT‑CAGAGATGAAATTTTGGCCACTCACGAGTg                                                            >  2:3065990/1‑93 (MQ=255)

                              tttcttCCCAGGCGAGTCTGAGTATATGAAAGACGCGCATTTGTTATCATCACCCCTGAATT‑CAGAGATGAAATTTTGGCCACTCACGAGTgg                                                           >  2:327939/1‑93 (MQ=255)

                              tttcttCCCAGGCGAGTCTGAGTATATGAAAGACGCGCATTTGTTATCATCACCCCTGAATT‑CAGAGATGAAATTTTGGCCACTCACGAGTgg                                                           >  2:849772/1‑93 (MQ=255)

                               ttcttcCCAGGCGAGTCTGAGTATATGAAAGACGCGCATTTGTTATCATCACCCCTGAATT‑CAGAGATGAAATTTTGGCCACTCACGAGTGGc                                                          >  1:4427602/1‑93 (MQ=255)

                                 cttcCCAGGCGAGTCTGAGTATATGAAAGACGCGCATTTGTTATCATCACCCCTGAATT‑CAGAGATGAAATTTTGGCCACTCACGAGTGGCCt                                                        <  1:2179202/93‑1 (MQ=255)

                                 cttcCCAGGCGAGTCTGAGTATATGAAAGACGCGCATTTGTTATCATCACCCCTGAATT‑CAGAGATGAAATTTTGGCCACTCACGAGTGGCCt                                                        <  1:3508795/93‑1 (MQ=255)

                                  ttcCCAGGCGAGTCTGAGTATATGAAAGACGCGCATTTGTTATCATCACCCCTGAATT‑CAGAGATGAAATTTTGGCCACTCACGAGTGGCCtt                                                       >  2:2011778/1‑93 (MQ=255)

                                  ttcCCAGGCGAGTCTGAGTATATGAAAGACGCGCATTTGTTATCATCACCCCTGAATT‑CAGAGATGAAATTTTGGCCACTCACGAGTGGCCtt                                                       <  1:3233899/93‑1 (MQ=255)

                                    cccAGGCGAGTCTGAGTATATGAAAGACGCGCATTTGTTATCATCACCCCTGAATT‑CAGAGATGAAATTTTGGCCACTCACGAGTGGCCtttt                                                     >  2:534049/1‑93 (MQ=255)

                                    cccAGGCGAGTCTGAGTATATGAAAGACGCGCATTTGTTATCATCACCCCTGAATT‑CAGAGATGAAATTTTGGCCACTCACGAGTGGCCtttt                                                     >  1:4539166/1‑93 (MQ=255)

                                     ccAGGCGAGTCTGAGTATATGAAAGACGCGCATTTGTTATCATCACCCCTGAATT‑CAGAGATGAAATTTTGGCCACTCACGAGTGGCCttttt                                                    >  2:3467627/1‑93 (MQ=255)

                                     ccAGGCGAGTCTGAGTATATGAAAGACGCGCATTTGTTATCATCACCCCTGAATT‑CAGAGATGAAATTTTGGCCACTCACGAGTGGCCttttt                                                    >  2:3161064/1‑93 (MQ=255)

                                       aGGCGAGTCTGAGTATATGAAAGACGCGCATTTGTTATCATCACCCCTGAATT‑CAGAGATGAAATTTTGGCCACTCACGAGTGGCCTttttct                                                  >  1:674875/1‑93 (MQ=255)

                                        ggCGAGTCTGAGTATATGAAAGACGCGCATTTGTTATCATCACCCCTGAATT‑CAGAGATGAAATTTTGGCCACTCACGAGTGGCCTttttctt                                                 >  1:209571/1‑93 (MQ=255)

                                           gAGTCTGAGTATATGAAAGACGCGCATTTGTTATCATCACCCCTGAATT‑CAGAGATGAAATTTTGGCCACTCACGAGTGGCCTttttcttttc                                              <  2:3961338/93‑1 (MQ=255)

                                                tGAGTATATGAAAGACGCGCATTTGTTATCATCACCCCTGAATT‑CAGAGATGAAATTTTGGCCACTCACGAGTGGCCTTTTTCTTTTCTGTCa                                         >  1:2074462/1‑93 (MQ=255)

                                                tGAGTATATGAAAGACGCGCATTTGTTATCATCACCCCTGAATT‑CAGAGATGAAATTTTGGCCACTCACGAGTGGCCTTTTTCTTTTCTGTCa                                         <  2:4195000/93‑1 (MQ=255)

                                                 gAGTATATGAAAGACGCGCATTTGTTATCATCACCCCTGAATT‑CAGAGATGAAATTTTGGCCACTCACGAGTGGCCTTTTTCTTTTCTGTCAg                                        <  1:2917474/93‑1 (MQ=255)

                                                  aGTATATGAAAGACGCGCATTTGTTATCATCACCCCTGAATT‑CAGAGATGAAATTTTGGCCACTCACGAGTGGCCTTTTTCTTTTCTGTCAgg                                       >  2:3266536/1‑93 (MQ=255)

                                                   gTATATGAAAGACGCGCATTTGTTATCATCACCCCTGAATT‑CAGAGATGAAATTTTGGCCACTCACGAGTGGCCTTTTTCTTTTCTGTCAGGc                                      >  1:1600108/1‑93 (MQ=255)

                                                   gTATATGAAAGACGCGCATTTGTTATCATCACCCCTGAATT‑CAGAGATGAAATTTTGGCCACTCACGAGTGGCCTTTTTCTTTTCTGTCAGGc                                      >  2:4329801/1‑93 (MQ=255)

                                                   gTATATGAAAGACGCGCATTTGTTATCATCACCCCTGAATT‑CAGAGATGAAATTTTGGCCACTCACGAGTGGCCTTTTTCTTTTCTGTCAGGc                                      >  2:790659/1‑93 (MQ=255)

                                                    tataTGAAAGACGCGCATTTGTTATCATCACCCCTGAATT‑CAGAGATGAAATTTTGGCCACTCACGAGTGGCCTTTTTCTTTTCTGTCAGGCg                                     <  2:2585479/93‑1 (MQ=255)

                                                    tataTGAAAGACGCGCATTTGTTATCATCACCCCTGAATT‑CAGAGATGAAATTTTGGCCACTCACGAGTGGCCTTTTTCTTTTCTGTCAGGCg                                     <  2:2365103/93‑1 (MQ=255)

                                                    tataTGAAAGACGCGCATTTGTTATCATCACCCCTGAATT‑CAGAGATGAAATTTTGGCCACTCACGAGTGGCCTTTTTCTTTTCTGTCAGGCg                                     >  1:698038/1‑93 (MQ=255)

                                                     atatGAAAGACGCGCATTTGTTATCATCACCCCTGAATT‑CAGAGATGAAATTTTGGCCACTCACGAGTGGCCTTTTTCTTTTCTGTCAGGCgt                                    >  1:3073588/1‑93 (MQ=255)

                                                     atatGAAAGACGCGCATTTGTTATCATCACCCCTGAATT‑CAGAGATGAAATTTTAGCCACTCACGAGTGGCCTTTTTCTTTTCTGTCAGGCgt                                    >  2:1020538/1‑93 (MQ=255)

                                                      tatGAAAGACGCGCATTTGTTATCATCACCCCTGAATT‑CAGAGATGAAATTTTGGCCACTCACGAGTGGCCTTTTTCTTTTCTGTCAGGCgtg                                   >  1:2255441/1‑93 (MQ=255)

                                                        tGAAAGACGCGCATTTGTTATCATCACCCCTGAATT‑CAGAGATGAAATTTTGGCCACTCACGAGTGGCCTTTTTCTTTTCTGTCAGGCGTGtt                                 >  2:1351008/1‑93 (MQ=255)

                                                         gAAAGACGCGCATTTGTTATCATCACCCCTGAATT‑CAGAGATGAAATTTTGGCCACTCACGAGTGGCCTTTTTCTTTTCTGTCAGGCGTGttt                                <  2:804695/93‑1 (MQ=255)

                                                          aaaGACGCGCATTTGTTATCATCACCCCTGAATT‑CAGAGATGAAATTTTGGCCACTCACGAGTGGCCTTTTTCTTTTCTGTCAGGCGTGtttt                               <  1:2435053/93‑1 (MQ=255)

                                                           aaGACGCGCATTTGTTATCATCACCCCTGAATT‑CAGAGATGAAATTTTGGCCACTCACGAGTGGCCTTTTTCTTTTCTGTCAGGCGTGttttt                              <  2:2252061/93‑1 (MQ=255)

                                                           aaGACGCGCATTTGTTATCATCACCCCTGAATT‑CAGAGATGAAATTTTGGCCACTCACGAGTGGCCTTTTTCTTTTCTGTCAGGCGTGttttt                              >  2:1572438/1‑93 (MQ=255)

                                                            aGACGCGCATTTGTTATCATCACCCCTGAATT‑CAGAGATGAAATTTTGGCCACTCACGAGTGGCCTTTTTCTTTTCTGTCAGGCGTGTTTTTc                             >  2:4629356/1‑93 (MQ=255)

                                                            aGACGCGCATTTGTTATCATCACCCCTGAATT‑CAGAGATGAAATTTTGGCCACTCACGAGTGGCCTTTTTCTTTTCTGTCAGGCGTGTTTTTc                             >  1:719904/1‑93 (MQ=255)

                                                            aGACGCGCATTTGTTATCATCACCCCTGAATT‑CAGAGATGAAATTTTGGCCACTCACGAGTGGCCTTTTTCTTTTCTGTCAGGCGTGTTTTTc                             >  2:4068148/1‑93 (MQ=255)

                                                             gACGCGCATTTGTTATCATCACCCCTGAATT‑CAGAGATGAAATTTTGGCCACTCACGAGTGGCCTTTTTCTTTTCTGTCAGGCGTGttttttc                            >  2:1730736/1‑91 (MQ=255)

                                                             gACGCGCATTTGTTATCATCACCCCTGAATT‑CAGAGATGAAATTTTGGCCACTCACGAGTGGCCTTTTTCTTTTCTGTCAGGCGTGTTTTTcc                            <  2:538836/93‑1 (MQ=255)

                                                              aCGCGCATTTGTTATCATCACCCCTGAATT‑CAGAGATGAAATTTTGGCCACTCACGAGTGGCCTTTTTCTTTTCTGTCAGGCGTGTTTTTCCa                           >  1:3222085/1‑93 (MQ=255)

                                                               cgcgCATTTGTTATCATCACCCCTGAATT‑CAGAGATGAAATTTTGGCCACTCACGAGTGGCCTTTTTCTTTTCTGTCAGGCGTGTTTTTCCAg                          >  1:2472667/1‑93 (MQ=255)

                                                                gcgcATTTGTTATCATCACCCCTGAATT‑CAGAGATGAAATTTTGGCCACTCACGAGTGGCCTTTTTCTTTTCTGTCAGGCGTGTTTTTCCAGc                         <  1:3089408/93‑1 (MQ=255)

                                                                  gcATTTGTTATCATCACCCCTGAATT‑CAGAGATGAAATTTTAGCCAATCACGAGCGGACGTTTTCTTTTCTGTTAGGGGTGTTTTTTCAGTca                       >  2:2414327/1‑93 (MQ=255)

                                                                   cATTTGTTATCATCACCCCTGAATT‑CAGAGATGAAATTTTGGCCACTCACGAGTGGCCTTTTTCTTTTCTGTCAGGCGTGTTTTTCCAGCcac                      >  2:212449/1‑93 (MQ=255)

                                                                    aTTTGTTATCATCACCCCTGAATT‑CAGAGATGAAATTTTGGCCACTCACGAGTGGCCTTTTTCTTTTCTGTCAGGCGTGTTTTTCCAGCcaca                     <  1:4053345/93‑1 (MQ=255)

                                                                     tttGTTATCATCACCCCTGAATT‑CAGAGATGAAATTTTGGCCACTCACGAGTGGCCTTTTTCTTTTCTGTCAGGCGTGTTTTTCCAGCCacac                    <  1:4275378/93‑1 (MQ=255)

                                                                     tttGTTATCATCACCCCTGAATT‑CAGAGATGAAATTTTGGCCACTCACGAGTGGCCTTTTTCTTTTCTGTCAGGCGTGTTTTTCCAGCCacac                    <  2:3945368/93‑1 (MQ=255)

                                                                       tGTTATCATCACCCCTGAATT‑CAGAGATGAAATTTTGGCCACTCACGAGTGGCCTTTTTCTTTTCTGTCAGGCGTGTTTTTCCAGCCACACCg                  >  1:4549416/1‑93 (MQ=255)

                                                                        gTTATCATCACCCCTGAATT‑CAGAGATGAAATTTTGGCCACTCACGAGTGGCCTTTTTCTTTTCTGTCAGGCGTGTTTTTCCAGCCACACCGc                 <  2:4116152/93‑1 (MQ=255)

                                                                            tcatcaccCCTGAATT‑CAGAGATGAAATTTTGGCCACTCACGAGTGGCCTTTTTCTTTTCTGTCAGGCGTGTTTTTCCAGCCACACCGCAAAc             <  1:1846992/93‑1 (MQ=255)

                                                                             catcaccCCTGAATT‑CAGAGATGAAATTTTGGCCACTCACGAGTGGCCTTTTTCTTTTCTGTCAGGCGTGTTTTTCCAGCCACACCGCAAACg            <  2:3777013/93‑1 (MQ=255)

                                                                               tcaccCCTGAATT‑CAGAGATGAAATTTTGGCCACTCACGAGTGGCCTTTTTATTTTCTGTCAGGCGTGTTTTTCCAGCCACACCGCAAACGGt          >  2:4023689/1‑93 (MQ=255)

                                                                                 accccTGAATT‑CAGAGATGAAATTTTGGCCACTCACGAGTGGCCTTTTTCTTTTCTGTCAGGCGTGTTTTTCCAGCCACACCGCAAACGGTTc        >  1:2999234/3‑93 (MQ=255)

                                                                                  ccccTGAATT‑CAGAGATGAAATTTTGGCCACTCACGAGTGGCCTTTTTCTTTTCTGTCAGGCGTGTTTTTCCAGCCACACCGCAAACGGTTCg       >  1:4041018/2‑93 (MQ=255)

                                                                                   cccTGAATT‑CAGAGATGAAATTTTGGCCACTCACGAGTGGCCTTTTTCTTTTCTGTCAGGCGTGTTTTTCCAGCCACACCGCAAACGGTTCgg      >  1:2787302/1‑93 (MQ=255)

                                                                                    ccTGAATT‑CAGAGATGAAATTTTGGCCACTCACGAGTGGCCTTTTTCTTTTCTGTCAGGCGTGTTTTTCCAGCCACACCGCAAACGGTTCGGt     >  2:2877317/1‑93 (MQ=255)

                                                                                    ccTGAATT‑CAGAGATGAAATTTTGGCCACTCACGAGTGGCCTTTTTCTTTTCTGTCAGGCGTGTTTTTCCAGCCACACCGCAAACGGTTCGGt     >  2:363248/1‑93 (MQ=255)

                                                                                    ccTGAATT‑CAGAGATGAAATTTTGGCCACTCACGAGTGGCCTTTTTCTTTTCTGTCAGGCGTGTTTTTCCAGCCACACCGCAAACGGCTCGGt     >  2:3438463/1‑93 (MQ=255)

                                                                                    ccTGAATT‑CAGAGATGAAATTTTGGCCACTCACGAGTGGCCTTTTTCTTTTCTGTCAGGCGTGTTTTTCCAGCCACACCGCAAAAGGTTCgga     >  2:3934076/1‑92 (MQ=255)

                                                                                     cTGAATT‑CAGAGATGAAATTTTGGCCACTCACGAGTGGCCTTTTTCTTTTCTGTCAGGCGTGTTTTTCCAGCCACACCGCAAACGGTTCGGTa    >  2:4550379/1‑93 (MQ=255)

                                                                                     cTGAATT‑CAGAGATGAAATTTTGGCCACTCACGAGTGGCCTTTTTCTTTTCTGTCAGGCGTGTTTTTCCAGCCACACCGCAAACGGTTCGGTa    <  2:3398040/93‑1 (MQ=255)

                                                                                      tGAATT‑CAGAGATGAAATTTTGGCCACTCACGAGTGGCCTTTTTCTTTTCTGTCAGGCGTGTTTTTCCAGCCACACCGCAAACGGTTCGGTAt   <  2:2780784/93‑1 (MQ=255)

                                                                                       gAATT‑CAGAGATGAAATTTTGGCCACTCACGAGTGGCCTTTTTCTTTTCTGTCAGGCGTGTTTTTCCAGCCACACCGCAAACGGTTCGGTATc  <  1:3378630/93‑1 (MQ=255)

CGGTAATTTTTTTAAAAATTTTCTGAACTCTTTCTTCCCAGGCGAGTCTGAGTATATGAAAGACGCGCATTTGTTATCATCATCCCTGAATTCCAGAGATGAAATTTTGGCCACTCACGAGTGGCCTTTTTCTTTTCTGTCAGGCGTGTTTTTCCAGCCACACCGCAAACGGTTCGGTATC  >  NC_000913/2812766‑2812946

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`