BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	300,243	+G	coding (869/957 nt)	yagS ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	300,240	1	.	G	99.3%	427.7 / ‑3.8	139	coding (872/957 nt)	yagS	hypothetical protein
Reads supporting (aligned to +/- strand): ref base . (1/0); new base G (85/53); total (86/53)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.71e-01

Only 100 of 153 total aligned reads displayed.

TCAAATTTCATGCCTGTGCCCTCGCTTCAGCCAGTACGGAGGCAAGCGTTCGCTTCGCCAACAGGAGTTTAAAGGTGTTTTCAGCGGTGGGA‑T‑GGGCGCTGGCGAACAGCGTGTCATATACGGCCTGCGCCCCCTGGGATAGCTGAGCATCCGCAGCCTCAATGCGCCAGGGCTTATGTG  >  NC_000913/300148‑300327

tCAAATTTCATGCCTGTGCCCTCGCTTCAGCCAGTACGGAGGCAAGCGTTCGCTTCGCCAACAGGAGTTTAAAGGTGTTTTCAGCGGTGGGA‑t                                                                                          <  1:1215719/93‑1 (MQ=255)

tCAAATTTCATGCCTGTGCCCTCGCTTCAGCCAGTACGGAGGCAAGCGTTCGCTTCGCCAACAGGAGTTTAAAGGTGTTTTCAGCGGTGGGA‑t                                                                                          >  1:1798977/1‑93 (MQ=255)

 cAAATTTCATGCCTGTGCCCTCGCTTCAGCCAGTACGGAGGCAAGCGTTCGCTTCGCCAACAGGAGTTTAAAGGTGTTTTCAGCGGTGGGA‑T‑g                                                                                        <  1:3236184/93‑1 (MQ=255)

   aaTTTCATGCCTGTGCCCTCGCTTCAGCCAGTACGGAGGCAAGCGTTCGCTTCGCCAACAGGAGTTTAAAGGTGTTTTCAGCGGTGGGA‑T‑ggg                                                                                      >  1:3942782/1‑93 (MQ=255)

   aaTTTCATGCCTGTGCCCTCGCTTCAGCCAGTACGGAGGCAAGCGTTCGCTTCGCCAACAGGAGTTTAAAGGTGTTTTCAGCGGTGGGA‑T‑ggg                                                                                      >  2:113786/1‑93 (MQ=255)

     tttCATGCCTGTGCCCTCGCTTCAGCCAGTACGGAGGCAAGCGTTCGCTTCGCCAACAGGAGTTTAAAGGTGTTTTCAGCGGTGGGA‑T‑ggggc                                                                                    >  1:2857777/1‑91 (MQ=255)

     tttCATGCCTGTGCCCTCGCTTCAGCCAGTACGGAGGCAAGCGTTCGCTTCGCCAACAGGAGTTTAAAGGTGTTTTCAGCGGTGGGA‑T‑ggggc                                                                                    >  2:4281097/1‑91 (MQ=255)

         aTGCCTGTGCCCTCGCTTCAGCCAGTACGGAGGCAAGCGTTCGCTTCGCCAACAGGAGTTTAAAGGTGTTTTCAGCGGTGGGA‑TGGGGCGCTg                                                                                 <  2:2069607/93‑1 (MQ=255)

         aTGCCTGTGCCCTCGCTTCAGCCAGTACGGAGGCAAGCGTTCGCTTCGCCAACAGGAGTTTAAAGGTGTTTTCAGCGGTGGGA‑TGGGGCGCTg                                                                                 >  1:1685116/1‑93 (MQ=255)

          tGCCTGTGCCCTCGCTTCAGCCAGTACGGAGGCAAGCGTTCGCTTCGCCAACAGGAGTTTAAAGGTGTTTTCAGCGGTGGGA‑TGGGGCGCTgg                                                                                >  2:517162/1‑93 (MQ=255)

           gCCTGTGCCCTCGCTTCAGCCAGTACGGAGGCAAGCGTTCGCTTCGCCAACAGGAGTTTAAAGGTGTTTTCAGCGGTGGGA‑TGGGGCGCTGGc                                                                               >  1:4116556/1‑93 (MQ=255)

             cTGTGCCCTCGCTTCAGCCAGTACGGAGGCAAGCGTTCGCTTCGCCAACAGGAGTTTAAAGGTGTTTTCAGCGGTGGGA‑TGGGGCGCTGGCGa                                                                             >  1:2889975/1‑93 (MQ=255)

              tgtgCCCTCGCTTCAGCCAGTACGGAGGCAAGCGTTCGCTTCGCCAACAGGAGTTTAAAGGTGTTTTCAGCGGTGGGA‑TGGGGCGCTGGCGaa                                                                            >  1:1789736/1‑93 (MQ=255)

              tgtgCCCTCGCTTCAGCCAGTACGGAGGCAAGCGTTCGCTTCGCCAACAGGAGTTTAAAGGTGTTTTCAGCGGTGGGA‑TGGGGCGCTGGCGaa                                                                            >  2:3958819/1‑93 (MQ=255)

              tgtgCCCTCGCTTCAGCCAGTACGGAGGCAAGCGTTCGCTTCGCCAACAGGAGTTTAAAGGTGTTTTCAGCGGTGGGA‑TGGGGCGCTGGCGaa                                                                            >  2:1502833/1‑93 (MQ=255)

               gtgCCCTCGCTTCAGCCAGTACGGAGGCAAGCGTTCGCTTCGCCAACAGGAGTTTAAAGGTGTTTTCAGCGGTGGGA‑TGGGGCGCTGGCGAAc                                                                           <  1:30288/93‑1 (MQ=255)

               gtgCCCTCGCTTCAGCCAGTACGGAGGCAAGCGTTCGCTTCGCCAACAGGAGTTTAAAGGTGTTTTCAGCGGTGGGA‑TGGGGCGCTGGCGAAc                                                                           <  2:2810463/93‑1 (MQ=255)

                tgCCCTCGCTTCAGCCAGTACGGAGGCAAGCGTTCGCTTCGCCAACAGGAGTTTAAAGGTGTTTTCAGCGGTGGGA‑TGGGGCGCTGGCGAACa                                                                          <  1:3576736/93‑1 (MQ=255)

                 gCCCTCGCTTCAGCCAGTACGGAGGCAAGCGTTCGCTTCGCCAACAGGAGTTTAAAGGCGTTTTCAGCGGTGGGA‑TGGGGCGCTGGCGAACAg                                                                         >  1:2539384/1‑93 (MQ=255)

                  cccTCGCTTCAGCCAGTACGGAGGCAAGCGTTCGCTTCGCCAACAGGAGTTTAAAGGTGTTTTCAGCGGTGGGA‑TGGGGCGCTGGCGAACAGc                                                                        <  2:2323433/93‑1 (MQ=255)

                  cccTCGCTTCAGCCAGTACGGAGGCAAGCGTTCGCTTCGCCAACAGGAGTTTAAAGGTGTTTTCAGCGGTGGGA‑TGGGGCGCGGGCGAACAGc                                                                        >  1:3291887/1‑93 (MQ=255)

                   ccTCGCTTCAGCCAGTACGGAGGCAAGCGTTCGCTTCGCCAACAGGAGTTTAAAGGTGTTTTCAGCGGTGGGA‑TGGGGCGCTGGCGAACAGCg                                                                       >  1:4332528/1‑93 (MQ=255)

                   ccTCGCTTCAGCCAGTACGGAGGCAAGCGTTCGCTTCGCCAACAGGAGTTTAAAGGTGTTTTCAGCGGTGGGA‑TGGGGCGCTGGCGAACAGCg                                                                       <  2:2012927/93‑1 (MQ=255)

                    cTCGCTTCAGCCAGTACGGAGGCAAGCGTTCGCTTCGCCAACAGGAGTTTAAAGGTGTTTTCAGCGGTGGGA‑TGGGGCGCTGGCGAACAGCgt                                                                      >  2:1797818/1‑93 (MQ=255)

                    cTCGCTTCAGCCAGTACGGAGGCAAGCGTTCGCTTCGCCAACAGGAGTTTAAAGGTGTTTTCAGCGGTGGGA‑TGGGGCGCTGGCGAACAGCgg                                                                      >  2:4135979/1‑92 (MQ=255)

                    cTCGCTTCAGCCAGTACGGAGGCAAGCGTTCGCTTCGCCAACAGGAGTTTAAAGGTGTTTTCAGCGGAGGGA‑TGGGGCGCTGGCGAACAGCgg                                                                      >  2:729724/1‑92 (MQ=255)

                     tCGCTTCAGCCAGTACGGAGGCAAGCGTTCGCTTCGCCAACAGGAGTTTAAAGGTGTTTTCAGCGGTGGGA‑TGGGGCGCTGGCGAACAGCgtg                                                                     >  2:4430115/1‑93 (MQ=255)

                     tCGCTTCAGCCAGTACGGAGGCAAGCGTTCGCTTCGCCAACAGGAGTTTAAAGGTGTTTTCAGCGGTGGGA‑TGGGGCGCTGGCGAACAGCgtg                                                                     >  2:796585/1‑93 (MQ=255)

                        cTTCAGCCAGTACGGAGGCAAGCGTTCGCTTCGCCAACAGGAGTTTAAAGGTGTTTTCAGCGGTGGGA‑TGGGGCGCTGGCGAACAGCGTGTCa                                                                  >  1:3530911/1‑93 (MQ=255)

                        cTTCAGCCAGTACGGAGGCAAGCGTTCGCTTCGCCAACAGGAGTTTAAAGGTGTTTTCAGCGGTGGGA‑TGGGGCGCTGGCGAACAGCGTGTCa                                                                  <  1:2005580/93‑1 (MQ=255)

                        cTTCAGCCAGAACGGAGGCAAGCGTTCGCTTCGCCAACAGGAGTTTAAAGGTGTTTTCAGCGGTGGGA‑TGGGGCGCTGGCGAACAGCGTGTCa                                                                  >  2:2869908/1‑93 (MQ=255)

                         ttCAGCCAGTACGGAGGCAAGCGTTCGCTTCGCCAACAGGAGTTTAAAGGTGTTTTCAGCGGTGGGA‑TGGGGCGCTGGCGAACAGCGTGTCat                                                                 <  2:4031715/93‑1 (MQ=255)

                          tCAGCCAGTACGGAGGCAAGCGTTCGCTTCGCCAACAGGAGTTTAAAGGTGTTTTCAGCGGTGGGA‑TGGGGCGCTGGCGAACAGCGTGTCata                                                                <  2:1664444/93‑1 (MQ=255)

                          tCAGCCAGTACGGAGGCAAGCGTTCGCTTCGCCAACAGGAGTTTAAAGGTGTTTTCAGCGGTGGGA‑TGGGGCGCTGGCGAACAGCGTGTCata                                                                >  1:4145147/1‑93 (MQ=255)

                          tCAGCCAGTACGGAGGCAAGCGTTCGCTTCGCCAACAGGAGTTTAAAGGTGTTTTCAGCGGTGGGA‑TGGGGCGCTGGCGAACAGCGTGTCata                                                                <  2:3673602/93‑1 (MQ=255)

                          tCAGCCAGTACGGAGGCAAGCGTTCGCTTCGCCAACAGGAGTTTAAAGGTGTTTTCAGCGGTGGGA‑TGGGGCGCTGGCGAACAGCGTGTCata                                                                <  2:2604082/93‑1 (MQ=255)

                           cAGCCAGTACGGAGGCAAGCGTTCGCTTCGCCAACAGGAGTTTAAAGGTGTTTTCAGCGGTGGGA‑TGGGGCGCTGGCGAACAGCGTGTCatat                                                               <  1:854070/93‑1 (MQ=255)

                            aGCCAGTACGGAGGCAAGCGTTCGCTTCGCCAACAGGAGTTTAAAGGTGTTTTCAGCGGTGGGA‑TGGGGCGCTGGCGAACAGCGTGTCAtata                                                              >  1:96840/1‑93 (MQ=255)

                            aGCCAGTACGGAGGCAAGCGTTCGCTTCGCCAACAGGAGTTTAAAGGTGTTTTCAGCGGTGGCA‑TGGGGCGCTGGCGAACAGCGTGTCAtata                                                              <  2:3125149/93‑1 (MQ=255)

                             gCCAGTACGGAGGCAAGCGTTCGCTTCGCCAACAGGAGTTTAAAGGTGTTTTCAGCGGTGGGA‑TGGGGCGCTGGCGAACAGCGTGTCATATAc                                                             <  1:531632/93‑1 (MQ=255)

                              ccAGTACGGAGGCAAGCGTTCGCTTCGCCAACAGGAGTTTAAAGGTGTTTTCAGCGGTGGGA‑TGGGGCGCTGGCGAACAGCGTGTCATATACg                                                            <  2:4634925/93‑1 (MQ=255)

                              ccAGTACGGAGGCAAGCGTTCGCTTCGCCAACAGGAGTTTAAAGGTGTTTTCAGCGGTGGGA‑TGGGGCGCTGGCGAACAGCGTGTCATATACg                                                            >  2:2678836/1‑93 (MQ=255)

                                aGTACGGAGGCAAGCGTTCGCTTCGCCAACAGGAGTTTAAAGGTGTTTTCAGCGGTGGGA‑TGGGGCGCTGGCGAACAGCGTGTCATATACGGc                                                          >  2:2529911/1‑93 (MQ=255)

                                 gTACGGAGGCAAGCGTTCGCTTCGCCAACAGGAGTTTAAAGGTGTTTTCAGCGGTGGGA‑TGGGGCGCTGGCGAACAGCGTGTCATATACGGcc                                                         <  2:368270/93‑1 (MQ=255)

                                 gTACGGAGGCAAGCGTTCGCTTCGCCAACAGGAGTTTAAAGGTGTTTTCAGCGGTGGGA‑TGGGGCGCTGGCGAACAGCGTGTCATATACGGcc                                                         >  2:1890250/1‑93 (MQ=255)

                                 gTACGGAGGCAAGCGTTCGCTTCGCCAACAGGAGTTTAAAGGTGTTTTCAGCGGTGGGA‑TGGGGCGCTGGCGAACAGCGTGTCATATACGGcc                                                         >  2:1488675/1‑93 (MQ=255)

                                  tACGGAGGCAAGCGTTCGCTTCGCCAACAGGAGTTTAAAGGTGTTTTCAGCGGTGGGA‑TGGGGCGCTGGCGAACAGCGTGTCATATACGGCCt                                                        >  2:13564/1‑93 (MQ=255)

                                  tACGGAGGCAAGCGTTCGCTTCGCCAACAGGAGTTTAAAGGTGTTTTCAGCGGTGGGA‑TGGGGCGCTGGCGAACAGCGTGTCATATACGGCCt                                                        <  1:1758090/93‑1 (MQ=255)

                                  tACGGAGGCAAGCGTTCGCTTCGCCAACAGGAGTTTAAAGGTGTTTTCAGCGGTGGGA‑TGGGGCGCTGGCGAACAGCGTGTCATATACGGCCt                                                        <  2:1061080/93‑1 (MQ=255)

                                  tACGGAGGCAAGCGTTCGCTTCGCCAACAGGAGTTTAAAGGTGTTTTCAGCGGTGGGA‑TGGGGCGCTGGCGAACAGCGTGTCATATACGGCCt                                                        >  1:278672/1‑93 (MQ=255)

                                   aCGGAGGCAAGCGTTCGCTTCGCCAACAGGAGTTTAAAGGTGTTTTCAGCGGTGGGA‑TGGGGCGCTGGCGAACAGCGTGTCATATACGGCCTg                                                       <  1:608968/93‑1 (MQ=255)

                                      gAGGCAAGCGTTCGCTTCGCCAACAGGAGTTTAAAGGTGTTTTCAGCGGTGGGA‑TGGGGCGCTGGCGAACAGCGTGTCATATACGGCCTgcgc                                                    >  1:1962511/1‑93 (MQ=255)

                                            aGCGTTCGCTTCGCCAACAGGAGTTTAAAGGTGTTTTCAGCGGTGGGA‑TGGGGCGCTGGCGAACAGCGTGTCATATACGGCCTGCGCCCCCTg                                              <  2:1357186/93‑1 (MQ=255)

                                            aGCGTTCGCTTCGCCAACAGGAGTTTAAAGGAGTTTTCAGCGGTGGGA‑TGGGGCGCTGGCGAACAGCGTGTCATATACGGCCTGCGGCCCCTg                                              >  2:2431554/1‑93 (MQ=255)

                                             gCGTTCGCTTCGCCAACAGGAGTTTAAAGGTGTTTTCAGCGGTGGGA‑TGGGGCGCTGGCGAACAGCGTGTCATATACGGCCTGCCCCCCCTgg                                             >  1:1536256/1‑93 (MQ=255)

                                             gCGTTCCCTTCGCCAACAGGAGTTTAAAGGTGTTTTCAGCGGTGGAA‑TGGGGCGCTGGCGAACAGCTTGTCATATCCGGCCTGCGCCCCCTgg                                             <  2:3543138/93‑1 (MQ=255)

                                               gTTCGCTTCGCCAACAGGAGTTTAAAGGTGTTTTCAGCGGTGGGA‑TGGGGCGCTGGCGAACAGCGTGTCATATACGGCCTGCGCCCCCTGGGa                                           >  1:3234238/1‑93 (MQ=255)

                                               gTTCGCTTCGCCAACAGGAGTTTAAAGGTGTTTTCAGCGGCGGGA‑TGGGGCGCTGGCGAACAGCGTGTCATATACGGCCTGCGCCCCCTGGGa                                           >  2:4644667/1‑93 (MQ=255)

                                                ttcgcttcgcCAACAGGAGTTTAAAGGTGTTTTCAGCGGTGGGA‑TGGGGCGCTGGCGAACAGCGTGTCATATACGGCCTGCGCCCCCTGGGAt                                          >  1:1095326/1‑93 (MQ=255)

                                                ttcgcttcgcCAACAGGAGTTTAAAGGTGTTTTCAGCGGTGGGA‑TGGGGCGCTGGCGAACAGCGTGTCATATACGGCCTGCGCCCCCTGGGAt                                          >  1:1390188/1‑93 (MQ=255)

                                                ttcgcttcgcCAACAGGAGTTTAAAGGTGTTTTCAGCGGTGGGA‑TGGGGCGCTGGCGAACAGCGTGTCATATACGGCCTGCGCCCCCTGGGAt                                          >  1:974518/1‑93 (MQ=255)

                                                 tcgcttcgcCAACAGGAGTTTAAAGGTGTTTTCAGCGGTGGGA‑TGGGGCGCTGGCGAACAGCGTGTCATATACGGCCTGCGCCCCCTGGGATa                                         <  2:1895469/93‑1 (MQ=255)

                                                 tcgcttcgcCAACAGGAGTTTAAAGGTGTTTTCAGCGGTGGGA‑TGGGGCGCTGGCGAACAGCGTGTCATATACGGCCTGCGCCCCCTGGGATa                                         >  2:2986987/1‑93 (MQ=255)

                                                 tcgcttcgcCAACAGGAGTTTAAAGGTGTTTTCAGCGGTGGGA‑TGGGGCGCTGGCGAACAGCGTGTCATATACGGCCTGCGCCCCCTGGGATa                                         >  1:661762/1‑93 (MQ=255)

                                                     ttcgcCAACAGGAGTTTAAAGGTGTTTTCAGCGGTGGGA‑TGGGGCGCTGGCGAACAGCGTGTCATATACGGCCTGCGCCCCCTGGGATAGCTg                                     >  1:1735499/1‑93 (MQ=255)

                                                        gcCAACAGGAGTTTAAAGGTGTTTTCAGCGGTGGGA‑TGGGGCGCTGGCGAACAGCGTGTCATATACGGCCTGCGCCCCCTGGGATAGCTGAGc                                  >  2:3267237/1‑93 (MQ=255)

                                                        gcCAACAGGAGTTTAAAGGTGTTTTCAGCGGTGGGA‑TGGGGCGCTGGCGAACAGCGTGTCATATACGGCCTGCGCCCCCTGGGATAGCTGAGc                                  >  2:2125981/1‑93 (MQ=255)

                                                         ccAACAGGAGTTTAAAGGTGTTTTCAGCGGTGGGA‑TGGGGCGCTGGCGAACAGCGTGTCATATACGGCCTGCGCCCCCTGGGATAGCTGAGCa                                 <  2:4360221/93‑1 (MQ=255)

                                                         ccAACAGGAGTTTAAAGGTGTTTTCAGCGGTGGGA‑TGGGGCGCTGGCGAACAGCGTGTCATATACGGCCTGCGCCCCCTGGGATAGCTGAGCa                                 <  1:4616372/93‑1 (MQ=255)

                                                          cAACAGGAGTTTAAAGGTGTTTTCAGCGGTGGGA‑TGGGGCGCTGGCGAACAGCGTGTCATATACGGCCTGCGCCCCCTGGGATAGCTGAGCAt                                <  2:3382488/93‑1 (MQ=255)

                                                          cAACAGGAGTTTAAAGGTGTTTTCAGCGGTGGGA‑TGGGGCGCTGGCGAACAGCGTGTCATATACGGCCTGCGCCCCCTGGGATAGCTGAGCAt                                >  2:4254659/1‑93 (MQ=255)

                                                           aaCAGGAGTTTAAAGGTGTTTTCAGCGGTGGGA‑TGGGGCGCTGGCGAACAGCGTGTCATATACGGCCTGCGCCCCCTGGGATAGCTGAGCATc                               <  1:3850095/93‑1 (MQ=255)

                                                           aaCAGGAGTTTAAAGGTGTTTTCAGCGGTGGGA‑TGGGGCGCTGGCGAACAGCGTGTCATATAAGGCCTGCGCCCCCTGGGATAGCTGAGCATc                               >  1:1412841/1‑93 (MQ=255)

                                                            aCAGGAGTTTAAAGGTGTTTTCAGCGGTGGGA‑TGGGGCGCTGGCGAACAGCGTGTCATATACGGCCTGCGCCCCCTGGGATAGCTGAGCATcc                              >  2:275669/1‑93 (MQ=255)

                                                            aCAGGAGTTTAAAGGTGTTTTCAGCGGTGGGA‑TGGGGCGCTGGCGAACAGCGTGTCATATACGGCCTGCGCCCCCTGGGATAGCTGAGCATcc                              >  2:3920501/1‑93 (MQ=255)

                                                            aCAGGAGTTTAAAGGTGTTTTCAGCGGTGGGA‑TGGGGCGCTGGCGAACAGCGTGTCATATACGGCCTGCGCCCCCTGGGATAGCTGAGCATcc                              >  1:4575730/1‑93 (MQ=255)

                                                             cAGGAGTTTAAAGGTGTTTTCAGCGGTGGGA‑TGGGGCGCTGGCGACCAGCGTGTCATATACGGCCTGCGCCCCCTGGGATACATGAGCATCCg                             >  2:1162669/1‑93 (MQ=255)

                                                              aGGAGTTTAAAGGTGTTTTCAGCGGTGGGA‑TGGGGCGCTGGCGAACAGCGTGTCATATACGGCCTGCGCCCCCTGGGATAGCTGAGCATCCGc                            >  2:3555681/1‑93 (MQ=255)

                                                              aGGAGTTTAAAGGTGTTTTCAGCGGTGGGA‑TGGGGCGCTGGCGAACAGCGTGTCATATACGGCCTGCGCCCCCTGGGATAGCTGAGCATCCGc                            >  1:1312086/1‑93 (MQ=255)

                                                              aGGAGTTTAAAGGTGTTTTCAGCGGTGGGA‑TGGGGCGCTGGCGAACAGCGTGTCATATACGGCCTGCGCCCCCTGGGATAGCTGAGCATCCGc                            >  1:3633266/1‑93 (MQ=255)

                                                                gAGTTTAAAGGTGTTTTCAGCGGTGGGA‑TGGGGCGCTGGCGAACAGCGTGTCATATACGGCCTGCGCCCCCTGGGATAGCTGAGCATCCGCAg                          <  1:868752/93‑1 (MQ=255)

                                                                  gTTTAAAGGTGTTTTCAGCGGTGGGA‑TGGGGCGCTGGCGAACAGCGTGTCATATACGGCCTGCGCCCCCTGGGATAGCTGAGCATCCGCAGcc                        >  2:899508/1‑93 (MQ=255)

                                                                  gTTTAAAGGTGTTTTCAGCGGTGGGA‑TGGGGCGCTGGCGAACAGCGTGTCATATACGGCCTGCGCCCCCTGGGATAGCTGAGCATCCGCAGcc                        <  2:334658/93‑1 (MQ=255)

                                                                  gTTTAAAGGTGTTTTCAGCGGTGGGA‑TGGGGCGCTGGCGAACAGCGTGTCATATACGGCCTGCGCCCCCTGGGATAGCTGAGCATCCGCAGcc                        >  1:4664349/1‑93 (MQ=255)

                                                                   tttAAAGGTGTTTTCAGCGGTGGGA‑TGGGGCGCTGGCGAACAGCGTGTCATATACGGCCTGCGCCCCCTGGGATAGCTGAGCATCCGCAGCCt                       >  1:3080159/1‑93 (MQ=255)

                                                                     tAAAGGTGTTTTCAGCGGTGGGA‑TGGGGCGCTGGCGAACAGCGTGTCATATACGGCCTGCGCCCCCTGGGATAGGTAAGCATCCGCCGCCTCa                     >  1:74135/1‑93 (MQ=255)

                                                                     tAAAGGTGTTTTCAGCGGTGGGA‑TGGGGCGCTGGCGAACAGCGTGTCATATACGGCCTGCGCCCCCTGGGATAGCTGAGCATCCGCAGCCTCa                     >  1:3994878/1‑93 (MQ=255)

                                                                       aaGGTGTTTTCAGCGGTGGGA‑TGGGGCGCTGGCGAACAGCGTGTCATATACGGCCTGCGCCCCCTGGGATAGTTGGGAATCCCCAGCCTCaag                   >  1:1554293/1‑92 (MQ=255)

                                                                       aaGGTGTTTTCAGCGGTGGGA‑TGGGGCGCTGGCGAACAGCGTGTCATATACGGCCTGCGCCCCCTGGGATAGCTGAGCATCCGCAGCCTCAAt                   >  1:2747975/1‑93 (MQ=255)

                                                                        aGGTGTTTTCAGCGGTGGGACG‑GGGCGCTGGCGAACAGCGTGTCATATACGGCCTGCGGCCCCTGGGATAGCTGAGCATCCGCAGCCTCAATg                  >  1:3309462/1‑93 (MQ=255)

                                                                         ggTGTTTTCAGCGGTGGGA‑TGGGGCGCTGGCGAACAGCGTGTCATATACGGCCTGCGCCCCCTGGGATAGCTGAGCATCCGCAGCCTCAATgc                 >  2:523014/1‑93 (MQ=255)

                                                                            gtTTTCAGCGGTGGGA‑TGGGGCGCTGGCGAACAGCGTGTCATATACGGCCTGCGCCCCCTGGGATAGCTGAGCATCCGCAGCCTCAATGCGcc              <  2:4183373/93‑1 (MQ=255)

                                                                            gtTTTCAGCGGTGGGA‑TGGGGCGCTGGCGAACAGCGTGTCATATACGGCCTGCGCCCCCTGGGATAGCTGAGCATCCGCAGCCTCAATGCGcc              >  2:1961121/1‑93 (MQ=255)

                                                                             ttttCAGCGGTGGGA‑TGGGGCGCTGGCGAACAGCGTGTCATATACGGCCTGCGCCCCCTGGGATAGCTGAGCATCCGCAGCCTCAATGCGCCa             >  1:713212/1‑93 (MQ=255)

                                                                             ttttCAGCGGTGGGA‑TGGGGCGCTGGCGAACAGCGTGTCATATACGGCCTGCGCCCCCTGGGATAGCTGAGCATCCGCACCCTCAATgcgcaa             >  1:1015745/1‑91 (MQ=255)

                                                                              tttCAGCGGTGGGA‑TGGGGCGCTGGCGAACAGCGTGTCATATACGGCCTGCGCCCCCTGGGATAGCTGAGCATCCGCAGCCTCAATGCGCCAg            <  2:4060020/93‑1 (MQ=255)

                                                                               ttCAGCGGTGGGA‑TGGGGCGCTGGCGAACAGCGTGTCATATACGGCCTGCGCCCCCTGGGATAGCTGAGCATCCGCAGCCTCAATGCGCCAgg           <  2:2228462/93‑1 (MQ=255)

                                                                                tCAGCGGTGGGA‑TGGGGCGCTGGCGAACAGCGTGTCATATACGGCCTGCGCCCCCTGGGATAGCTGAGCATCCGCAGCCTCAATGCGCCAggg          >  1:1647790/1‑93 (MQ=255)

                                                                                     ggTGGGA‑TGGGGCGCTGGCGAACAGCGTGTCATATACGGCCTGCGCCCCCTGGGATAGCTGAGCATCCGCAGCCTCAATGCGCCAGGGCTTAt     <  2:3624713/93‑1 (MQ=255)

                                                                                        gggA‑TGGGGCGCTGGCGAACAGCGTGTCATATACGGCCTGCGCCCCCTGGGATAGCTGAGCATCCGCAGCCTCAATGCGCCAGGGCTTAtgtg  >  2:3993640/1‑93 (MQ=255)

TCAAATTTCATGCCTGTGCCCTCGCTTCAGCCAGTACGGAGGCAAGCGTTCGCTTCGCCAACAGGAGTTTAAAGGTGTTTTCAGCGGTGGGA‑T‑GGGCGCTGGCGAACAGCGTGTCATATACGGCCTGCGCCCCCTGGGATAGCTGAGCATCCGCAGCCTCAATGCGCCAGGGCTTATGTG  >  NC_000913/300148‑300327

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 18 ≤` `ATCG/ATCG` `< 26 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`