BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,175,525	(C)_6→7	coding (402/630 nt)	yqiE ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,175,519	1	.	C	92.5%	463.7 / 31.7	160	coding (408/630 nt)	yqiE	hypothetical protein
Reads supporting (aligned to +/- strand): ref base . (4/8); new base C (67/80); total (72/88)
Fisher's exact test for biased strand distribution p-value = 5.50e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.83e-01

Only 100 of 189 total aligned reads displayed.

GAATATCTTCGTTTTCATCAGCCAGACCGTGAATACCGCTTGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGGT‑G‑CCCCC‑CGGGCTTGCCAGGAAACTTAACACCGGTTTGGTCCGTTTGACTATCAGTCCCGCCTCTTCAATCGCTTCGCGACGGGCAACA  >  NC_000913/3175428‑3175606

   tctCTTCGTTTTCATCAGCCAGACCGTGAATACCGCTTGCGGTCTTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGGT‑G‑cccc                                                                                      <  2:4235167/91‑1 (MQ=255)

   tatCTTCTTTTTCACCAGCCAGACCGTGAATACCGCTTGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGGT‑G‑cccc                                                                                      <  2:2487000/93‑1 (MQ=255)

   tatCTTCGTTTTCATCAGCCAGACCGTGAATACCGCTTGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGGT‑G‑cccc                                                                                      >  1:4202206/1‑93 (MQ=255)

   tatCTTCGTTTTCATCAGCCAGACCGTGAATACCGCTTGCGGTCGTGGCGTCCACTTCGCCCACAATAATTGACGAACGCTCACTGGT‑G‑cccc                                                                                      >  1:1008051/1‑93 (MQ=255)

    atCTTCGTTTTCATCAGCCAGACCGTGAATACCGCTTGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGGT‑G‑ccccc                                                                                     >  1:2640044/1‑93 (MQ=255)

    atCTTCGTTTTCATCAGCCAGACCGTGAATACCGCTTGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGGT‑G‑ccccc                                                                                     >  2:1435799/1‑93 (MQ=255)

     tCTTCGTTTTCATCAGCCAGCCCGTGAATCCCGCTTGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGGT‑G‑ccccc‑c                                                                                   <  2:4446644/93‑1 (MQ=255)

     tCTTCGTTTTCATCAGCCAGCCCGTGAATACCGCTTGCGGTCGTGGCGTCCCCTTCGCCCACCATAATTGACGAACGCTCACTGGT‑G‑ccccc‑c                                                                                   <  2:1762207/93‑1 (MQ=255)

     tCTTCGTTTTCATCAGCCAGACCGTGAATCCCGCTTGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGGT‑G‑ccccc‑c                                                                                   <  2:462683/93‑1 (MQ=255)

     tCTTCGTTTTCATCAGCCAGACCGTGAATACCGCTTGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGGT‑G‑ccccc‑c                                                                                   >  1:3592131/1‑93 (MQ=255)

     tCTTCGTTTTCATCAGCCAGACCGTGAATACCGCTTGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGGT‑G‑ccccc‑c                                                                                   >  1:4213438/1‑93 (MQ=255)

      tttCGTTTTCATCAGCCAGACCGTGAATACCGCTTGCGGTCGTGGCGTCCTCTCCGCCCACCATAATTGACGAACGCTCACTGGT‑G‑ccccc‑cc                                                                                  <  2:3501783/92‑2 (MQ=255)

       ttCTTTTGCATCAGCCGGACCGTGAATCCCGCTTGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGGT‑G‑ccccc‑ccg                                                                                 <  1:4444684/93‑3 (MQ=255)

        tCGTTTTCATCAGCCAGACCGTGAATCCCGCTTGCGGTCGTGGCGTCCCCTCCGCCCACCATAATTGACGAACGCTCACTGGT‑G‑CCCCC‑Ccgg                                                                                <  2:1528486/93‑1 (MQ=255)

        tCGTTTTCATCAGCCAGACCGTGAATACCGCTTGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGGT‑G‑CCCCC‑Ccgg                                                                                <  1:4478989/93‑1 (MQ=255)

        tCGTTTTCATCAGCCAGACCGTGAATACCGCTTGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGGT‑G‑CCCCC‑Ccgg                                                                                >  2:4521689/1‑93 (MQ=255)

        tCGTTTTCATCAGCCAGACCGTGAATACCGCTTGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGGT‑G‑CCCCC‑Ccgg                                                                                >  2:585318/1‑93 (MQ=255)

        tCGTTTTCATCAGCCAGACCGTGAATACCGCTTGCGGTCGTGGCGTCCACTCCGCCCACCATAATTGACGAACGCTCACTGGT‑G‑CCCCC‑Ccgg                                                                                <  2:2657898/93‑1 (MQ=255)

          gTTTTCATCAGCCAGACCGTGAATCCCGCTTGCGGTCGTGGCTCCCACTTCGCCCACCATAATTGACGAACGCTCACTGGT‑G‑CCCCC‑Ccgggc                                                                              <  1:2983759/93‑3 (MQ=255)

           ttttCATCAGCCAGCCGGTGAATCCCGCTTGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGGT‑GCCCCCC‑CGGGCt                                                                              <  2:1788918/93‑1 (MQ=255)

           ttttCATCAGCCAGACCGTGAATACCGCTTGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGGT‑GCCCCCC‑CGGGCt                                                                              >  1:4429624/1‑93 (MQ=255)

              tcatcaGCCAGACCGTGAATACCGCTTGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGGT‑GCCCCCC‑CGGGCTTGc                                                                           >  1:1467217/1‑93 (MQ=255)

               catcaGCCAAACCTGAAATACCGCTTGGGTTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGGT‑G‑CCCCCGCGGGCTTGcc                                                                          <  2:2869650/93‑1 (MQ=255)

                atcaGCCAGACCGTGAATACCGCTTGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGGT‑GCCCCCC‑CGGGCTTGCCa                                                                         <  1:1491657/93‑1 (MQ=255)

                atcaGCCAGACCGTGAATACCGCTTGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGGT‑GCCCCCC‑CGGGCTTGCCa                                                                         >  1:1346020/1‑93 (MQ=255)

                 tcaGCCAGACCGTGAATACCGCTTGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGGT‑GCCCCCC‑CGGGCTTGCCAg                                                                        >  1:2783493/1‑93 (MQ=255)

                 tcaCCCAGCCGGTGAATACCGCTTGCGGTCGTGGCGTCCCCTTCGCCCACCATAATTGACGAACGCTCACTGGT‑GCCCCCC‑CGGGCTTGCCAg                                                                        <  1:3441169/93‑1 (MQ=255)

                    gCCAGACCGTGAATACCGCTTGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGGT‑GCCCCCC‑CGGGCTTGCCAGGaa                                                                     <  1:4643446/93‑1 (MQ=255)

                    accAGCCCGTGAAGCCCGCTTGCGGTCGTGGCGTCCCCTTCGCCCACCATAATTGACGAACGCTCACTGGT‑GCCCCCC‑CGGGCTTGCCAGGaa                                                                     <  2:2116582/92‑1 (MQ=255)

                      cAGACCGTGAATCCCGCTTGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGGT‑GCCCCCC‑CGGGCTTGCCAGGAAAc                                                                   <  1:1689270/93‑1 (MQ=255)

                      cAGACCGTGAATACCGCTTGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGGT‑GCCCCCC‑CGGGCTTGCCAGGAAAc                                                                   >  2:2793293/1‑93 (MQ=255)

                      cAGACCGTGAATACCGCTTGCGGTCGTGGCGCCCACTTCGCCCACCATAATTGACGAACGCTCACTGGT‑GCCCCCC‑CGGGCTTGCCAGGAAAc                                                                   <  1:787058/93‑1 (MQ=255)

                       aGACGGTGAATCCCGTTGGCGGTCGTGGCGTCCCCTTCGCCCACCATAATTGACGAACGCTCACTGGT‑GCCCCCC‑CGGGCTTGCCAGGAAACt                                                                  <  2:2691991/93‑1 (MQ=255)

                       aGACCGTGAATCCCGTTTGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGGT‑GCCCCCC‑CGGGCTTGCCAGGAAACt                                                                  <  1:2312760/93‑1 (MQ=255)

                        gACCGTGAATACCGCTTGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGGT‑GCCCCCC‑CGGGCTTGCCAGGAAACtt                                                                 >  1:3172295/1‑93 (MQ=255)

                        gACCGTGAATACCGCTTGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGGT‑GCCCCCC‑CGGGCTTGCCAGGAAACtt                                                                 >  2:485748/1‑93 (MQ=255)

                         aCCGTGAATACCGCTTGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGGT‑GCCCCCC‑CGGGCTTGCCAGGAAACTTa                                                                >  2:1179775/1‑93 (MQ=255)

                          cggTGAACGCCGCTCGCGGTCGTGGCGGCCACTCCGCCCACCATAATTGACGAACGCTCACTGGT‑GCCCCCC‑CGGGCTTGCCAGGAAACTTaa                                                               <  2:3563112/91‑1 (MQ=255)

                          ccGTGAATACCGCTTGCGGTCGTGGCGTCCACTTCGCCAACCATAATTGACGAACGCTCACTGGT‑GCCCCCC‑CGGGCTTGCCAGGAAACTTaa                                                               <  1:1996729/93‑1 (MQ=255)

                           cGTGAATACCGTTGGCGGTGGTGGCTTCCTCTCCGCCCACCATAATTGACGAACGCTCACTGGT‑GCCCCCC‑CGGGCTTGCCAGGAAACTTAac                                                              <  1:4023340/93‑1 (MQ=255)

                           cGTGAATACCGCTTGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGGT‑GCCCCCC‑CGGGCTTGCCAGGAAACTTAac                                                              >  2:1929397/1‑93 (MQ=255)

                           cGTGAATACCGCTTGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGGT‑GCCCCCC‑CGGGCTTGCCAGGAAACTTAac                                                              >  1:475379/1‑93 (MQ=255)

                           cGTGAATACCGCTTGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGGT‑GCCCCCC‑CGGGCTTGCCAGGAAACTTAac                                                              >  2:3032850/1‑93 (MQ=255)

                            gTGAATACCGCTTGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGGT‑GCCCCCC‑CGGGCTTGCCAGGAAACTTAaca                                                             >  2:1035339/1‑93 (MQ=255)

                             tGAATACCGCTTGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGGT‑GCCCCCC‑CGGGCTTGCCAGGAAACTTAacac                                                            >  1:1012293/1‑93 (MQ=255)

                              gAATACCGCTTGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGGT‑GCCCCCC‑CGGGCTTGCCAGGACACTTAACAcc                                                           >  2:828874/1‑93 (MQ=255)

                              gAATACCGCTTGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGGT‑GCCCCCC‑CGGGCTTGCCAGGAAACTTAACAcc                                                           >  2:1082742/1‑93 (MQ=255)

                                aTACCGCTTGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGGT‑GCCCCCC‑CGGGCTTGCCAGGAAACTTAACACCgg                                                         >  2:934116/1‑93 (MQ=255)

                                aTACCGCTTGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGGT‑GCCCCCC‑CGGGCTTGCCAGGAAACTTAACACCgg                                                         >  1:1837910/1‑93 (MQ=255)

                                 tcccGCTGGCGGTGGTGGCGTCCACTCCGCCCACCATAATTGACGAACGCTCACTGGT‑GCCCCCC‑CGGGCTTGCCAGGAAACTTAACACCGGt                                                        <  2:1838137/91‑1 (MQ=255)

                                  aCCGCTTGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGGT‑GCCCCCC‑CGGGCTTGCCAGGAAACTTAACACCGGtt                                                       >  2:1905701/1‑93 (MQ=255)

                                    ggCTTGCGTGCGTGGCGCCCACTTCGCCCACCATAATTGACGAACGCTCACTGGT‑GCCCCCC‑CGGGCTTGCCAGGAAACTTAACACCGGTTTg                                                     <  1:2132764/92‑1 (MQ=255)

                                    cGCTTGCGTGCGTGGCGACCACTTCCCCCACCATAATTGACGAACGCTCACTGGT‑GCCCCCC‑CGGGCTTGCCAGGAAACTTAACACCGGTTTg                                                     <  2:2173736/93‑1 (MQ=255)

                                     ccTTGGGGTCTTGTCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGGT‑GCCCCCC‑CGGGCTTGCCAGGAAACTTAACACCGGTTTgg                                                    <  1:2222416/92‑1 (MQ=255)

                                       ttGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGGT‑GCCCCCC‑CGGGCTTGCCAGGAAACTTAACACCGGTTTGGTc                                                  >  2:2254256/1‑93 (MQ=255)

                                       ttGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGGT‑GCCCCCC‑CGGGCTTGCCAGGAAACTTAACACCGGTTTGGTc                                                  >  1:1899701/1‑93 (MQ=255)

                                        tGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGGT‑GCCCCCC‑CGGGCTTGCCAGGAAACTTAACACCGGTTTGGTcc                                                 >  1:2472929/1‑93 (MQ=255)

                                             tCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGGT‑GCCCCCC‑CGGGCTTGCCAGGAAACTTAACACCGGTTTGGTCCGTTTg                                            >  2:3827827/1‑93 (MQ=255)

                                             ccGTGGCGCCCACTGCGCCCACCATAATTGACGAACGCTCACTGGT‑GCCCCCC‑CGGGCTTGCCAGGAAACTTAACACCGGTTTGGTCCGTTTg                                            <  1:885416/92‑1 (MQ=255)

                                                 ggCGTCCACTCCGCCCACCATAATTGACGAACGCTCACTGGT‑GCCCCCC‑CGGGCTTGCCAGGAAACTTAACACCGGTTTGGTCCGTTTGACTa                                        <  2:2005356/93‑1 (MQ=255)

                                                   cGTCCACTTCCCCCACCATAATTGACGAACGCGCACTGGT‑GCCCCCC‑CGGGCTTGCCAGGAAACTTAACACCGGTTTGGTCCGTTTGACTATc                                      <  1:1522198/93‑1 (MQ=255)

                                                    gTCCACTTCGCCCACCATAATTGACGAACGCTCACTGGT‑GCCCCCC‑CGGGCTTGCCAGGAAACTTAACACCGGTTTGGTCCGTTTGACTATCa                                     >  2:539802/1‑93 (MQ=255)

                                                    gTCCACTTCGCCCACCATAATTGACGAACGCTCACTGGT‑GCCCCCC‑CGGGCTTGCCAGGAAACTTAACACCGGTTTGGTCCGTTTGACTATCa                                     >  1:659744/1‑93 (MQ=255)

                                                     tCCACTTCGCCCACCATAATTGACGAACGCTCACTGGT‑GCCCCCC‑CGGGCTTGCCAGGAAACTTAACACCGGTTTGGTCCGTTTGACTATCAg                                    >  1:3846556/1‑93 (MQ=255)

                                                      ccccTTCGCCCACCATAATTGACGAACGCTCACTGGT‑GCCCCCC‑CGGGCTTGCCAGGAAACTTAACACCGGTTTGGTCCGTTTGACTATCAGt                                   <  1:2447944/90‑1 (MQ=255)

                                                      ccACTCCCCCCACCATAATTGACGAACGCTCACTGGT‑GCCCCCC‑CGGGCTTGCCAGGAAACTTAACACCGGTTTGGTCCGTTTGACTATCAGt                                   <  1:3377405/93‑1 (MQ=255)

                                                       cACTTCGCCCACCATAATTGACGAACGCTCACTGGT‑GCCCCCC‑CGGGCTTGCCAGGAAACTTAACACCGGTTTGGTCCGTTTGACTATCAGTc                                  <  1:4498921/93‑1 (MQ=255)

                                                         cTTCGCCCACCATAATTGACGAACGCTCACTGGT‑GCCCCCC‑CGGGCTTGCCAGGAAACTTAACACCGGTTTGGTCCGTTTGACTATCAGTccc                                >  2:3229805/1‑93 (MQ=255)

                                                          ctCGCCCACCATAATTGACGAACGCTCACTGGT‑GCCCCCC‑CGGGCTTGCCAGGAAACTTAACACCGGTTTGGTCCGTTTGACTATCAGTCCCg                               <  1:2582484/92‑1 (MQ=255)

                                                             ccccACCATAATTGACGAACGCTCACTGGT‑GCCCCCC‑CGGGCTTGCCAGGAAACTTAACACCGGTTTGGTCCGTTTGACTATCAGTCCCGCct                            <  2:2883740/92‑1 (MQ=255)

                                                              cccACCATAATTGACGAACGCTCACTGGT‑GCCCCCC‑CGGGCTTGCCAGGAAACTTAACACCGGTTTGGTCCGTTTGACTATCAGTCCCGCctc                           >  2:3152288/1‑93 (MQ=255)

                                                              cccACCATAATTGACGAACGCTCACTGGT‑GCCCCCC‑CGGGCTTGCCAGGAAACTTAACACCGGTTTGGTCCGTTTGACTATCAGTCCCGCctc                           >  1:2023583/1‑93 (MQ=255)

                                                               ccaccaTAATTGACTAACGCTCACTGGT‑GCCCCCC‑CGGGCTTGCCAGGAAACTTAACACCGGTTTGGTCCGTTTGACTATCAGTCCCGCctct                          <  1:4553171/93‑1 (MQ=255)

                                                                caccaTAATTGACGAACGCTCACTGGT‑GCCCCCC‑CGGGCTTGCCAGGAAACTTAACACCGGTTTGGTCCGTTTGACTATCAGTCCCGCCTCtt                         >  1:1567163/1‑93 (MQ=255)

                                                                caccaTAATTGACGAACGCTCACTGGT‑GCCCCCC‑CGGGCTTGCCAGGAAACTTAACACCGGTTTGGTCCGTTTGACTATCAGTCCCGCCTCtt                         >  1:964379/1‑93 (MQ=255)

                                                                caccaTAATTGACGAACGCTCACTGGT‑GCCCCCC‑CGGGCTTGCCAGGAAACTTAACACCGGTTTGGTCCGTTTGACTATCAGTCCCGCCTCtt                         >  1:4252039/1‑93 (MQ=255)

                                                                    aTAATTGACGAACGCTCACTGGT‑GCCCCCC‑CGGGCTTGCCAGGAAACTTAACACCGGTTTGGTCCGTTTGACTATCAGTCCCGCCTCTTCAAt                     <  2:3429387/93‑1 (MQ=255)

                                                                     tAATTGACGAACGCTCACTGGT‑GCCCCCC‑CGGGCTTGCCAGGAAACTTAACACCGGTTTGGTCCGTTTGACTATCAGTCCCGCCTCTTCAATc                    <  1:1969254/93‑1 (MQ=255)

                                                                     tAATTGACGAACGCTCACTGGT‑GCCCCCC‑CGGGCTTGCCAGGAAACTTAACACCGGGTTGGTCCGTTTGACTATCCGTCCCGCCTCTTCAATc                    >  1:4053408/1‑93 (MQ=255)

                                                                        ttGACGAACGCTCACTGGT‑GCCCCCC‑CGGGCTTGCCAGGAAACTTAACACCGGTTTGGTCCGTTTGACTATCAGTCCCGCCTCTTCAATCGCt                 <  1:3119357/93‑1 (MQ=255)

                                                                         tGACGAACGCTCACTGGT‑GCCCCCC‑CGGGCTTGCCAGGAAACTTAACCCCGGTTTGGTCCGTTTGACTATCAGTCCCGCCTCTTCAATCGCtt                <  2:4108264/93‑1 (MQ=255)

                                                                         tGACGAACGCTCACTGGT‑GCCCCCC‑CGGGCTTGCCAGGAAACTTAACACCGGTTTGGTCCGTTTGACTATCAGTCCCGCCTCTTCAATCGCtt                <  1:3128933/93‑1 (MQ=255)

                                                                         tGACGAACGCTCACTGGT‑GCCCCCC‑CGGGCTTGCCAGGAAACTTAACACCGGTTTGGTCCGTTTGACTATCAGTCCCGCCTCTTCAATCGCtt                <  2:3642747/93‑1 (MQ=255)

                                                                          gACGAACGCTCACTGGT‑GCCCCCC‑CGGGCTTGCCAGGAAACTTAACACCGGTTTGGTCCGTTTGACTATCAGTCCCGCCTCTTCAATCGCTTc               <  2:2073360/93‑1 (MQ=255)

                                                                            cGAACGCTCACTGGT‑GCCCCCC‑CGGGCTTGCCAGGAAACTTAACACCGGTTTGGTCCGTTTGACTATCAGTCCCGCCTCTTCAATCGCTTcgc             <  1:1827584/93‑1 (MQ=255)

                                                                             gAACGCTCACTGGT‑GCCCCCC‑CGGGCTTGCCAGGAAACTTAACCCCGGTTTGGTCCGTTTGACTATCAGTCCCGCCTCTTCAATCGCTTcgcg            <  2:2288193/93‑1 (MQ=255)

                                                                               aCGCTCACTGGT‑GCCCCCC‑CGGGCTTGCCAGGAAACTTAACACCGGTTTGGTCCGTTTGACTATCAGTCCCGCCTCTTCAATCGCTTCGCGAc          <  2:1353648/93‑1 (MQ=255)

                                                                               aCGCTCACTGGT‑GCCCCCC‑CGGGCTTGCCAGGAAACTTAACACCGGTTTGGTCCGTTTGACTATCAGTCCCGCCTCTTCAATCGCTTCGCGAc          <  1:3643412/93‑1 (MQ=255)

                                                                                cGCTCACTGGT‑GCCCCCC‑CGGGCTTGCCAGGAAACTTAACACCGGTTTGGTCCGTTTGCCTATCAGTCCCGCCTCTTCAATCGCTTCGCGACg         <  1:3263876/93‑1 (MQ=255)

                                                                                 gCTCACTGGT‑GCCCCCC‑CGGGCTTGCCAGGAAACTTAACACCGGTTTGGTCCTTTTGACTATCAGTCCCGCCTCTTCAATCGCTTCGCGACgg        <  1:3723567/93‑1 (MQ=255)

                                                                                 gCTCACTGGT‑GCCCCCC‑CGGGCTTGCCAGGAAACTTAACACCGGTTTGGTCCGTTTGACTATCAGTCCCGCCTCTTCAATCGCTTCGCGACgg        >  1:2913892/1‑93 (MQ=255)

                                                                                  cTCACTGGTCC‑CCCCC‑CGGGCTTGCCAGGAAACTTACCACCGGTTTGGTCCGTTTGACTATCAGTCCCGCCTCTTCAATCGCTTCGCGACggg       <  2:1624959/93‑1 (MQ=255)

                                                                                  cTCACTGGT‑GCCCCCC‑CGGGCTTGCCAGGAAACTTAACACCGGTTTGGTCCGTTTGACTATCAGTCCCGCCTCTTCAATCGCTTCGCGACggg       >  1:43461/1‑93 (MQ=255)

                                                                                   tCACTGGT‑GCCCCCC‑CGGGCTTGCCAGGAAACTTAACACCGGTTTGGTCCGTTTGACTATCAGTCCCGCCTCTTCAATCGCTTCGCGACGGGc      >  2:903947/1‑93 (MQ=255)

                                                                                    cACTGGT‑GCCCCCC‑CGGGCTTGCCAGGAAACTTAACACCGGTTTGGTCCGTTTGACTATCAGTCCCGCCTCTTCAATCGCTTCGCGACGGGCa     >  2:622902/1‑93 (MQ=255)

                                                                                     aCTGGT‑GCCCCCC‑CGGGCTTGCCAGGAAACTTAACACCGGTTTGGTCCGTTTGACTATCAGTCCCGCCTCTTCAATCGCTTCGCGACGGGCaa    <  1:1095450/93‑1 (MQ=255)

                                                                                      cTGGT‑GCCCCCC‑CGGGCTTGCCAGGAAACTTAACACCGGTTTGGTCCGTTTGACTATCAGTCCCGCCTCTTCAATCGCTTCGCGACGGGCAAc   >  1:929967/1‑93 (MQ=255)

                                                                                      cTGGT‑GCCCCCC‑CGGGCTTGCCAGGAAACTTAACACCGGTTTGGTCCGTTTGACTATCAGTCCCGCCTCTTCAATCGCTTCGCGACGGGCAAc   <  1:719190/93‑1 (MQ=255)

                                                                                       tGGT‑GCCCCCC‑CGGGCTTGCCAGGAAACTTAACACCGGTTTGGTCCGTTTGACTATCAGTCCCGCCTCTTCAATCGCTTCGCGACGGGCAACa  <  1:3868787/93‑1 (MQ=255)

                                                                                       tGGT‑GCCCCCC‑CGGGCTTGCCAGGAAACTTAACACCGGTTTGGTCCGTTTGACTATCAGTCCCGCCTCTTCAATCGCTTCGCGACGGGCAACa  <  1:641031/93‑1 (MQ=255)

GAATATCTTCGTTTTCATCAGCCAGACCGTGAATACCGCTTGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGGT‑G‑CCCCC‑CGGGCTTGCCAGGAAACTTAACACCGGTTTGGTCCGTTTGACTATCAGTCCCGCCTCTTCAATCGCTTCGCGACGGGCAACA  >  NC_000913/3175428‑3175606

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 16 ≤` `ATCG/ATCG` `< 24 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`