BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,184,181	(T)_5→6	intergenic (+200/‑22)	ygiL → / → yi215	putative fimbrial‑like protein/IS2 hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,184,176	1	.	T	100.0%	114.9 / NA	39	intergenic (+195/‑27)	ygiL/yi215	putative fimbrial‑like protein/IS2 hypothetical protein
Reads supporting (aligned to +/- strand): ref base . (0/0); new base T (34/5); total (34/5)

AAAATATTTATTGTTCATTATCCGTTATTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAG‑TTTTTAGACTAGTCATTGGAGAACA  >  NC_000913/3184084‑3184201

aaaaTATTTTTTTTTCATTATCCGTAATTAGACT‑GCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAG‑t                          <  2:3267083/93‑1 (MQ=17)

 aaaTATTTATTGTTCATTATCCGTTATTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAG‑t                          <  2:1344334/93‑1 (MQ=255)

  aaTATTTATTGTTCATTATCCGTTATTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAG‑tt                         <  1:669106/93‑1 (MQ=255)

  aaTATTTATTGTTCATTATCCGTTATTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAG‑tt                         >  2:3171548/1‑93 (MQ=255)

  aaTATTTATTGTTCATTATCCGTTATTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAG‑tt                         <  1:1763225/93‑1 (MQ=255)

  aaTATTTATTGTTCATTATCCGTTATTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAG‑tt                         >  2:2147158/1‑93 (MQ=255)

  aaTATTTATTGTTCATTATCCGTTATTAGACTGGCCCCCTCAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAG‑tt                         <  2:2643180/93‑1 (MQ=255)

    tatttattGTTCATTATCCGTTATTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAG‑tttt                       <  1:1586767/93‑1 (MQ=255)

    tatttattGTTCATTATCCGTTATTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAG‑tttt                       >  2:1293993/1‑93 (MQ=255)

    tatttattGTTCATTATCCGTTATTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAG‑tttt                       >  2:2394654/1‑93 (MQ=255)

      tttattGTTCATTATCCGTTATTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAG‑tttttt                     >  2:3506107/1‑92 (MQ=255)

       ttattGTTCATTATCCGTTATTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAG‑tttttta                    >  2:538137/1‑91 (MQ=255)

       ttattGTTCATTATCCGTTATTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAG‑tttttta                    <  2:2855126/93‑3 (MQ=255)

       ttattGTTCATTATCCGTTATTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAG‑tttttta                    <  1:3212041/93‑3 (MQ=255)

       ttattGTTCATTATCCGTTATTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAG‑tttttta                    >  2:1346493/1‑91 (MQ=255)

        tattGTTCATTATCCGTTATTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAG‑ttttttag                   <  2:886634/93‑4 (MQ=255)

         attGTTCATTATCCGTTATTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAG‑ttttttaga                  >  2:2186234/1‑89 (MQ=255)

         attGTTCATTATCCGTTATTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAG‑ttttttaga                  >  2:2777329/1‑89 (MQ=255)

         attGTTCATTATCCGTTATTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAG‑ttttttaga                  >  1:4607725/1‑89 (MQ=255)

           tGTTCATTATCCGTTATTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTTAGACt                 <  1:1390135/93‑1 (MQ=255)

           tGTTCATTATCCGTTATTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTTAGACt                 <  1:1426817/93‑1 (MQ=255)

            gTTCATTATCCGTTATTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTTAGACTa                >  1:2441267/1‑93 (MQ=255)

            gTTCATTATCCGTTATTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTTAGACTa                >  1:3932771/1‑93 (MQ=255)

            gTTCATTATCCGTTATTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTTAGACTa                >  1:711254/1‑93 (MQ=255)

            gTTCATTATCCGTTATTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTTAGACTa                >  1:2818288/1‑93 (MQ=255)

             ttCATTATCCGTTATTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTTAGACTAg               >  2:94523/1‑93 (MQ=255)

             ttCATTATCCGTTATTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTTAGACTAg               >  1:2650730/1‑93 (MQ=255)

             ttCATTATCCGTTATTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTTAGACTAg               >  1:2412433/1‑93 (MQ=255)

             ttCATTATCCGTTATTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTTAGACTAg               >  1:1682323/1‑93 (MQ=255)

              tCATTATCCGTTATTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTTAGACTAGt              >  2:1304292/1‑93 (MQ=255)

              tCATTATCCGTTATTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTTAGACTAGt              >  2:1002206/1‑93 (MQ=255)

                aTTATCCGTTATTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTTAGACTAGTct            >  2:504625/1‑92 (MQ=255)

                 ttATCCGTTATTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTTAGACTAGTCAt           >  2:3059092/1‑93 (MQ=255)

                 ttATCCGTTATTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTTAGACTAGTCAt           >  2:3229342/1‑93 (MQ=255)

                  tATCCGTTATTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTTAGACTAGTCAtt          >  1:264245/1‑93 (MQ=255)

                   aTCCGTTATTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTTAGACTAGTCATTg         <  2:2601440/93‑1 (MQ=255)

                   aTCCGTTATTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTTAGACTAGTCATTg         >  2:2431564/1‑93 (MQ=255)

                    tCCGTTATTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTTAGACTAGTCATTgg        >  1:3975990/1‑93 (MQ=255)

                    tCCGTTATTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTTAGACTAGTCATTgg        >  2:212901/1‑93 (MQ=255)

                    tCCGTTATTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTTAGACTAGTCATTgg        >  2:1859406/1‑93 (MQ=255)

                    tCCGTTATTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTTAGACTAGTCATTgg        >  2:1207051/1‑93 (MQ=255)

                     ccGTTATTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTTAGACTAGTCATTggg       >  2:869220/1‑92 (MQ=255)

                     ccGTTATTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTTAGACTAGTCATTGga       >  1:2379110/1‑93 (MQ=255)

                     ccGTTATTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTTAGACTAGTCATTGga       <  1:2024599/93‑1 (MQ=255)

                      cGTTATTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTTAGACTAGTCATTGgag      >  2:1718826/1‑93 (MQ=255)

                       gTTATTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTTAGACTAGTCATTGgaga     >  2:1260566/1‑93 (MQ=255)

                       gTTATTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTTAGACTAGTCATTGgaga     >  2:1171095/1‑93 (MQ=255)

                        ttattaGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTTAGACTAGTCATTGGAGaa    >  2:1338026/1‑93 (MQ=16)

                        ttattaGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTTAGACTAGTCATTGGAGaa    >  1:848050/1‑93 (MQ=16)

                        ttattaGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTTAGACTAGTCATTGGAGaa    >  2:4670554/1‑93 (MQ=16)

                        ttattaGACTAGCCCCCTGAATCTCCAAAAAAACAATATCACTTAAATAAGTGATAGTCTTAATAATAGTTTTTTAGACTAGTCATTGgagga    >  2:794359/1‑91 (MQ=11)

                         tattaGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTTAGACTAGTCATTGGAGAAc   >  1:2419783/1‑93 (MQ=12)

                         tattaGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTTAGACTAGTCATTGGAGAAc   >  1:1266060/1‑93 (MQ=12)

                         tattaGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTTAGACTAGTCATTGGAGAAc   >  1:3432842/1‑93 (MQ=12)

                         tattaGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTTAGACTAGTCATTGGAGAAc   >  1:539244/1‑93 (MQ=12)

                          attaGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTTAGACTAGTCATTGGAGAACa  >  2:4634366/1‑93 (MQ=9)

                          attaGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTTAGACTAGTCATTGGAGAACa  >  1:1604443/1‑93 (MQ=9)

                          attaGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTTAGACTAGTCATTGGAGAACa  <  1:1613467/93‑1 (MQ=9)

                          attaGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTTAGACTAGTCATTGGAGAACa  >  2:256564/1‑93 (MQ=9)

AAAATATTTATTGTTCATTATCCGTTATTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAG‑TTTTTAGACTAGTCATTGGAGAACA  >  NC_000913/3184084‑3184201

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 26 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`