BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,544,357	+G	coding (163/1314 nt)	gntT →	gluconate permease

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,544,356	1	.	G	99.4%	549.9 / NA	175	V54V (GTC→GTG)	gntT	gluconate permease
Reads supporting (aligned to +/- strand): ref base . (0/0); major base G (98/76); minor base T (1/0); total (99/76)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 186 total aligned reads displayed.

GCTTCATCGCTCTCGTCCTCGTGGCGCTTGCTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTC‑GC‑GGACG‑TCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCAGACTGCGGTGGCGCACAA  >  NC_000913/3544265‑3544437

  ttCATCGCTCTCGTCCTCGTGGCGCTTGCTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTc‑ggc                                                                                  >  1:4056639/1‑91 (MQ=255)

   tCATCGCTCTCGTCCTCGTGGCGCTTGCTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTc‑ggcg                                                                                 >  2:461339/1‑90 (MQ=255)

   tCATCGCTCTCGTCCTCGTGGCGCTTGCTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTc‑ggcg                                                                                 <  2:3847825/93‑4 (MQ=255)

                  tCGTGGCGCTTGCTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGGCGGGACGCTCGGTAGcc                                                                   <  2:4237415/93‑1 (MQ=255)

                  tCGTGGCGCTTGCTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGGCGGGACGCTCGGTAGcc                                                                   <  2:4193830/93‑1 (MQ=255)

                  tCGTGGCGCTTGCTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGGCGGGACGCTCGGTAGcc                                                                   >  2:3664150/1‑93 (MQ=255)

                    ggtgcgcTTGCTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGGCGGGACGCTCGGTAGCCtt                                                                 <  1:913828/90‑1 (MQ=255)

                    gTGGCGCTTGCTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGGCGGGACGCTCGGTAGCCtt                                                                 <  1:344648/93‑1 (MQ=255)

                     tGGCGCTTGCTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGGCGGGACGCTCGGTAGCCTTg                                                                <  1:3531934/93‑1 (MQ=255)

                     tGGCGCTTGCTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGGCGGGACGCTCGGTAGCCTTg                                                                >  2:155306/1‑93 (MQ=255)

                      ggCGCTTGCTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGGCGGGACGCTCGGTAGCCTTGc                                                               >  2:3917384/1‑93 (MQ=255)

                       gcgcTTGCTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGGCGGGACGCTCGGTAGCCTTGcc                                                              >  1:961876/1‑93 (MQ=255)

                       gcgcTTGCTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGGCGGGACGCTCGGTAGCCTTGcc                                                              >  2:1041893/1‑93 (MQ=255)

                        cgcTTGCTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGGCGGGACGCTCGGTAGCCTTGccc                                                             <  2:2613965/93‑1 (MQ=255)

                         gcTTGCTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGGCGGGACGCTCGGTAGCCTTGCCCt                                                            >  2:1655533/1‑93 (MQ=255)

                         gcTTGCTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGGCGGGACGCTCGGTAGCCTTGCCCt                                                            >  1:2380438/1‑93 (MQ=255)

                          cTTGCTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGGCGGGACGCTCGGTAGCCTTGCCCTg                                                           >  2:2576230/1‑93 (MQ=255)

                          cTTGCTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGGCGGGACGCTCGGTAGCCTTGCCCTg                                                           >  2:2741321/1‑93 (MQ=255)

                            tGCTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGGCGGGACGCTCGGTAGCCTTGCCCTGAt                                                         <  2:3061747/93‑1 (MQ=255)

                            tGCTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGGCGGGACGCTCGGTAGCCTTGCCCTGAt                                                         >  1:2207666/1‑93 (MQ=255)

                            tGCTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGGCGGGACGCTCGGTAGCCTTGCCCTGAt                                                         >  2:3083821/1‑93 (MQ=255)

                            tGCTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGGCGGGACGCTCGGTAGCCTTGCCCTGAt                                                         <  1:441007/93‑1 (MQ=255)

                             gCTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGGCGGGACGCTCGGTAGCCTTGCCCTGATc                                                        >  2:870751/1‑93 (MQ=255)

                              cTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGGCGGGACGCTCGGTAGCCTTGCCCTGATCa                                                       >  1:2337232/1‑93 (MQ=255)

                              cTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGGCGGGACGCTCGGTAGCCTTGCCCTGATCa                                                       <  1:46968/93‑1 (MQ=255)

                              cTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGGCGGGACGCTCGGTAGCCTTGCCCTGATCa                                                       <  2:2922078/93‑1 (MQ=255)

                               tGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGGCGGGACGCTCGGTAGCCTTGCCCTGATCAt                                                      <  1:3261377/93‑1 (MQ=255)

                               tGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGGCGGGACGCTCGGTAGCCTTGCCCTGATCAt                                                      >  1:761706/1‑93 (MQ=255)

                               tGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGGCGGGACGCTCGGTAGCCTTGCCCTGATCAt                                                      >  1:2919310/1‑93 (MQ=255)

                               tGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGGCGGGACGCTCGGTAGCCTTGCCCTGATCAt                                                      >  1:1916909/1‑93 (MQ=255)

                               tGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGGCGGGACGCTCGGTAGCCTTGCCCTGATCAt                                                      <  1:2081690/93‑1 (MQ=255)

                               tGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGGCGGGACGCTCGGTAGCCTTGCCCTGATCAt                                                      <  2:3078121/93‑1 (MQ=255)

                                gTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGGCGGGACGCTCGGTAGCCTTGCCCTGATCATg                                                     >  1:2822764/1‑93 (MQ=255)

                                gTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGGCGGGACGCTCGGTAGCCTTGCCCTGATCATg                                                     >  1:1848023/1‑93 (MQ=255)

                                gTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGGCGGGACGCTCGGTAGCCTTGCCCTGATCATg                                                     >  1:2476438/1‑93 (MQ=255)

                                gTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGGCGGGACGCTCGGTAGCCTTGCCCTGATCATg                                                     >  2:2946353/1‑93 (MQ=255)

                                gTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGGCGGGACGCTCGGTAGCCTTGCCCTGATCATg                                                     >  1:2778786/1‑93 (MQ=255)

                                 ttGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGGCGGGACGCTCGGTAGCCTTGCCCTGATCATgg                                                    >  1:1674786/1‑93 (MQ=255)

                                     aTTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGGCGGGACGCTCGGTAGCCTTGCCCTGATCATGGGttt                                                >  2:3726683/1‑93 (MQ=255)

                                     aTTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGGCGGGACGCTCGGTAGCCTTGCCCTGATCATGGGttt                                                >  2:1353914/1‑93 (MQ=255)

                                     aTTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGGCGGGACGCTCGGTAGCCTTGCCCTGATCATGGGttt                                                <  2:4113884/93‑1 (MQ=255)

                                      ttAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGGCGGGACGCTCGGTAGCCTTGCCCTGTTCATGGGtttt                                               >  2:3911158/1‑93 (MQ=255)

                                      ttAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGGCGGGACGCTCGGTAGCCTTGCCCTGATCATGGGtttt                                               >  2:2475478/1‑93 (MQ=255)

                                      ttAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGGCGGGACGCTCGGTAGCCTTGCCCTGATCATGGGtttt                                               >  2:1776139/1‑93 (MQ=255)

                                      ttAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGGCGGGACGCTCGGTAGCCTTGCCCTGATCATGGGtttt                                               >  1:1105909/1‑93 (MQ=255)

                                       tAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGGCGGGACGCTCGGTAGCCTTGCCCTGATCATGGGTTTTg                                              <  1:3258736/93‑1 (MQ=255)

                                         aTGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGGCGGGACGCTCGGTAGCCTTGCCCTGATCATGGGTTTTGgc                                            >  2:3570103/1‑93 (MQ=255)

                                         aTGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGGCGGGACGCTCGGTAGCCTTGCCCTGATCATGGGTTTTGgc                                            <  1:4488048/93‑1 (MQ=255)

                                         aTGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGGCGGGACGCTCGGTAGCCTTGCCCTGATCATGGGTTTTGgc                                            <  1:2970084/93‑1 (MQ=255)

                                         aTGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGGCGGGACGCTCGGTAGCCTTGCCCTGATCATGGGATTTGgc                                            >  2:4327467/1‑93 (MQ=255)

                                             aaGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGGCGGGACGCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCaa                                        <  1:415728/93‑1 (MQ=255)

                                              aGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGGCGGGACGCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAAt                                       <  1:941319/93‑1 (MQ=255)

                                              aGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGGCGGGACGCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAAt                                       >  1:553925/1‑93 (MQ=255)

                                              aGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGGCGGGACGCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAAt                                       <  1:2163505/93‑1 (MQ=255)

                                              aGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGGCGGGACGCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAAt                                       <  2:1794283/93‑1 (MQ=255)

                                               ggAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGGCGGGACGCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATg                                      >  2:4299924/1‑93 (MQ=255)

                                               ggAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGGCGGGACGCTCGGTAGCCTTGCCCTGATCATGGGGTTTGGCGGAATg                                      >  2:3610799/1‑93 (MQ=255)

                                                gAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGGCGGGACGCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGc                                     >  2:285672/1‑93 (MQ=255)

                                                gAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGGCGGGACGCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGc                                     >  1:1674847/1‑93 (MQ=255)

                                                 aaTGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGGCGGGACGCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCt                                    <  1:1183248/93‑1 (MQ=255)

                                                  aTGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGGCGGGACGCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTg                                   >  1:3340926/1‑93 (MQ=255)

                                                  aTGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGGCGGGACGCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTg                                   <  1:4683799/93‑1 (MQ=255)

                                                  aTGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGGCGGGACGCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTg                                   >  2:607116/1‑93 (MQ=255)

                                                  aTGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGGCGGGACGCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTg                                   <  1:998086/93‑1 (MQ=255)

                                                   tGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGGCGGGACGCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTgg                                  <  1:1334676/93‑1 (MQ=255)

                                                      cGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGGCGGGACGCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCa                               <  1:2720547/93‑1 (MQ=255)

                                                      cGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGGCGGGACGCTCGGTAGCCTTGCCCTGATCATGGGTTTTCGCGCAATGCTGGGCa                               >  2:744078/1‑93 (MQ=255)

                                                       gCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGGCGGGACGCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCaa                              >  1:4068175/1‑93 (MQ=255)

                                                       gCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGGCGGGACGCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCaa                              >  2:1598220/1‑93 (MQ=255)

                                                       gCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGGCGGGACGCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCaa                              >  2:3293537/1‑93 (MQ=255)

                                                       gCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGGCGGGACGCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCaa                              >  1:2115018/1‑93 (MQ=255)

                                                        cTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGGCGGGACGCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCaaa                             <  1:4581190/93‑1 (MQ=255)

                                                         tGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGGCGGGACGCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCaaaa                            <  1:1259065/93‑1 (MQ=255)

                                                         tGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGGCGGGACGCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCaaaa                            <  2:1527427/93‑1 (MQ=255)

                                                         tGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGGCGGGACGCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCaaaa                            >  2:767357/1‑93 (MQ=255)

                                                          ggATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGGCGGGACGCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAAt                           <  1:2840603/93‑1 (MQ=255)

                                                          ggATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGGCGGGACGCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAAt                           >  2:2354515/1‑93 (MQ=255)

                                                          ggATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGGCGGGACGCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAAt                           >  1:4327314/1‑93 (MQ=255)

                                                           gATAAATTTATTGGCTCCATCAAAGCCGGTGTCGGCGGGACGCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATg                          <  2:876233/93‑1 (MQ=255)

                                                            aTAAAGTTATTGGCTCCATCAAAGCCGGTGTCGGCGGGACGCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGc                         >  2:1719351/1‑93 (MQ=255)

                                                            aTAAAGTTATTGGCTCCATCAAAGCCGGTGTCGGCGGGACGCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGc                         <  2:3786462/93‑1 (MQ=255)

                                                             tAAAGTTATTGGCTCCATCAAAGCCGGTGTCGGCGGGACGCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCt                        <  2:3412009/93‑1 (MQ=255)

                                                               aaGTTATTGGCTCCATCAAAGCCGGTGTCGGCGGGACGCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTgg                      <  2:2224878/93‑1 (MQ=255)

                                                               aaGTTATTGGCTCCATCAAAGCCGGTGTCGGCGGGACGCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTgg                      >  1:3703496/1‑93 (MQ=255)

                                                                  ttATTGGCTCCATCAAAGCCGGTGTCGGCGGGACGCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCAg                   <  1:1016120/93‑1 (MQ=255)

                                                                    aTTGGCTCCATCAAAGCCGGTGTCGGCGGGACGCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCAGAc                 >  2:1132059/1‑93 (MQ=255)

                                                                     ttGGCTCCATCAAAGCCGGTGTCGGCGGGACGCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCAGACt                >  2:3169621/1‑93 (MQ=255)

                                                                       ggCTCCATCAAAGCCGGTGTCGGCGGGACGCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCAGACTgg              >  2:2835135/1‑92 (MQ=255)

                                                                        gCTCCATCAAAGCCGGTGTCGGCGGGACGCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCAGACTGCg             <  1:1458581/93‑1 (MQ=255)

                                                                        gCTCCATCAAAGCCGGTGTCGGCGGGACGCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCAGACTGCg             >  1:1230254/1‑93 (MQ=255)

                                                                          tCCATCAAAGCCGGTGTCGGCGGGACGCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCAGACTGCGGt           <  2:3403926/93‑1 (MQ=255)

                                                                          tCCATCAAAGCCGGTGTCGGCGGGACGCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCAGACTGCGGt           >  1:4387931/1‑93 (MQ=255)

                                                                           ccATCAAAGCCGGTGTCGGCGGGACGCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCAGACTGCGGTg          >  1:2254298/1‑93 (MQ=255)

                                                                           ccATCAAAGCCGGTGTCGGCGGGACGCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCAGACTGCGGTg          >  2:3267112/1‑93 (MQ=255)

                                                                            cATCAAAGCCGGTGTCGGCGGGACGCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCAGACTGCGGTgg         <  1:2035198/93‑1 (MQ=255)

                                                                              tCAAAGCCGGTGTCGGCGGGACGCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCAGACTGCGGTGgcg       <  1:2108631/93‑1 (MQ=255)

                                                                               cAAAGCCGGTGTCGGCGGGACGCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCAGACTGCGGTGgcgc      <  2:2049911/93‑1 (MQ=255)

                                                                                  aGCCGGTGTCGGCGGGACGCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCAGACTGCGGTGGCGcaca   <  1:2011048/93‑1 (MQ=255)

                                                                                  aGCCGGTGTCGGCGGGACGCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCAGACTGCGGTGGCGcaca   <  2:364621/93‑1 (MQ=255)

                                                                                   gCCGGTGTCGGCGGGACGCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCAGACTGCGGTGGCGCACaa  >  2:2560175/1‑93 (MQ=255)

GCTTCATCGCTCTCGTCCTCGTGGCGCTTGCTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTC‑GC‑GGACG‑TCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCAGACTGCGGTGGCGCACAA  >  NC_000913/3544265‑3544437

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`