Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,725,747	(C)8→9	coding (1254/1455 nt)	xylB ←	xylulokinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,725,739	1	.	C	85.9%	170.4 / 24.7	64	coding (1262/1455 nt)	xylB	xylulokinase
Reads supporting (aligned to +/- strand):  ref base . (2/7);  new base C (27/28);  total (29/35)
Fisher's exact test for biased strand distribution p-value = 1.66e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.85e-01

CTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGG‑‑CCC‑‑CA‑C‑A‑‑T‑‑CCCCC‑‑CCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCC  >  NC_000913/3725647‑3725821                                                                                                      |                                                                                      cTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGG‑‑CCC‑‑CA‑C‑A‑‑t                                                                                        >  1:3287730/1‑93 (MQ=255) cTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGG‑‑CCC‑‑CA‑C‑A‑‑t                                                                                        <  1:723482/93‑1 (MQ=255)  tAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGG‑‑CCC‑‑CA‑C‑A‑‑T‑‑c                                                                                     >  2:1673349/1‑93 (MQ=255)  tAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGG‑‑CCC‑‑CA‑C‑A‑‑T‑‑c                                                                                     >  2:2932576/1‑93 (MQ=255)  tAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGG‑‑CCC‑‑CA‑C‑A‑‑T‑‑c                                                                                     >  2:3299266/1‑93 (MQ=255)  tAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCAGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGG‑‑CCC‑‑CA‑C‑A‑‑T‑‑c                                                                                     >  1:1327921/1‑93 (MQ=255)   aaCGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGG‑‑CCC‑‑CA‑C‑A‑‑T‑‑cc                                                                                    >  2:2957500/1‑93 (MQ=255)    aCGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCCGCCTTGCTGCGCCCAGTGCTGG‑‑CCC‑‑CA‑C‑A‑‑T‑‑ccc                                                                                   >  1:2961727/1‑93 (MQ=255)    aCGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGG‑‑CCC‑‑CA‑C‑A‑‑T‑‑ccc                                                                                   >  2:657433/1‑93 (MQ=255)     cGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGG‑‑CCC‑‑CA‑C‑A‑‑T‑‑cccc                                                                                  >  1:1915957/1‑93 (MQ=255)     cGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGG‑‑CCC‑‑CA‑C‑A‑‑T‑‑cccc                                                                                  >  2:147920/1‑93 (MQ=255)       gTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGG‑‑CCC‑‑CA‑C‑A‑‑T‑‑ccccc‑‑c                                                                              >  1:1845408/1‑93 (MQ=255)       gTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGG‑‑CCC‑‑CA‑C‑A‑‑T‑‑ccccc‑‑c                                                                              <  2:3068541/93‑1 (MQ=255)       gTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGG‑‑CCC‑‑CA‑C‑A‑‑T‑‑ccccc‑‑c                                                                              >  1:1893691/1‑93 (MQ=255)       gTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGG‑‑CCC‑‑CA‑C‑A‑‑T‑‑ccccc‑‑c                                                                              >  1:2181050/1‑93 (MQ=255)          gTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGG‑‑CCC‑‑CA‑C‑A‑‑T‑‑ccccc‑‑cccc                                                                           <  2:3767173/93‑2 (MQ=255)          gTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGG‑‑CCC‑‑CA‑C‑A‑‑T‑‑ccccc‑‑cccc                                                                           >  1:2037365/1‑92 (MQ=255)          gTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGG‑‑CCC‑‑CA‑C‑A‑‑C‑‑ccccc‑‑cccc                                                                           >  2:3363473/1‑92 (MQ=255)           ttGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGG‑‑CCC‑‑CA‑C‑A‑‑T‑‑ccccc‑‑ccccc                                                                          >  2:4115099/1‑91 (MQ=255)           ttGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGG‑‑ACC‑‑CA‑C‑A‑‑T‑‑ccccc‑‑ccccc                                                                          >  1:373255/1‑91 (MQ=255)             gCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGG‑‑CCC‑‑CA‑C‑A‑‑T‑‑ccccc‑‑cccccgt                                                                        <  1:3779792/93‑5 (MQ=255)                  aacaaTTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGG‑‑CCC‑‑CA‑C‑A‑‑TC‑CCCCC‑‑CCCCTACGGTa                                                                    >  1:106043/1‑93 (MQ=255)                   acaaTTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGG‑‑CCC‑‑CA‑C‑A‑‑TCCCCCCC‑‑CCCGTACGGTa                                                                    >  2:4437149/1‑93 (MQ=255)                   acaaTTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCCCCAGCCTTGCTGCGCCCAGTTCTGG‑‑CCC‑‑CA‑C‑A‑‑TCCCCCCC‑‑CCCGTACGGTa                                                                    <  1:4136195/93‑1 (MQ=255)                       ttCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGG‑‑CCC‑‑CA‑C‑A‑‑TC‑CCCCC‑‑CCCGTACGGTAATCGa                                                               <  2:3188259/93‑1 (MQ=255)                       ttCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGG‑‑CCC‑‑CA‑C‑A‑‑TC‑CCCCC‑‑CCCCTACGGTAATTGa                                                               >  2:380092/1‑93 (MQ=255)                       ttCAATGAGCGATATCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGG‑‑CCC‑‑CA‑C‑A‑‑TC‑CCCCC‑‑CCCGTACGGTAATCGa                                                               <  1:2678715/93‑1 (MQ=255)                          aaTGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGG‑‑CCC‑‑CA‑C‑A‑‑TCCCCCCC‑‑CCCGTACGGTAATCGAgc                                                             >  2:2229547/1‑93 (MQ=255)                          aaTGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGG‑‑CCC‑‑CA‑C‑A‑‑TCCCCCCC‑‑CCCGTACGGTAATCGAgc                                                             <  1:4018573/93‑1 (MQ=255)                          aaTGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGG‑‑CCC‑‑CA‑C‑A‑‑TC‑CCCCC‑‑CCCGTACGGTAATCGAgct                                                            >  1:3132106/1‑93 (MQ=255)                            tGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGG‑‑CCC‑‑CA‑C‑A‑‑TCCCCCCC‑‑CCCGCACGGTAATCGAgctg                                                           >  1:1070663/1‑93 (MQ=255)                            tGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGG‑‑CCC‑‑CA‑C‑A‑‑TCCCCCCC‑‑CCCCCACAGTAATCGAgctg                                                           >  2:755066/1‑93 (MQ=255)                             gAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGG‑‑CCC‑‑CA‑C‑A‑‑TCCCCCCC‑‑CCCGTACGGTAATCGAgctgc                                                          >  1:4649719/1‑93 (MQ=255)                               gCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGG‑‑CCC‑‑CA‑C‑A‑‑TC‑CCCCC‑‑CCCGTACGGCACTCGAGCTGCTGa                                                       >  2:1924927/1‑93 (MQ=255)                                 gATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGG‑‑CCC‑‑CA‑C‑A‑‑TCCCCCCC‑‑CCCGTACGGGAATCGAGCGGCTGAc                                                      >  2:610587/1‑93 (MQ=255)                                 gATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGG‑‑CCC‑‑CA‑C‑A‑‑TCCCCCCC‑‑CCCGGACGGGAATCGGGCTGCTGAc                                                      >  2:461662/1‑93 (MQ=255)                                   tttCTCTGGATTCGCCGCGATCGGCGCCAGCCTTGCTGCGCCCAGTGCTGG‑‑CCC‑‑CA‑C‑A‑‑TC‑CCCCC‑‑CCCGTACGGTAATCGAGCTGCTGACCGc                                                   <  2:1254102/93‑1 (MQ=255)                                     tctcTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGG‑‑CCC‑‑CA‑C‑A‑‑TCCCCCCC‑‑CCCGTACGGTAATCGAGCTGCTGACCGCt                                                  >  2:802756/1‑93 (MQ=255)                                      ctctGGCTTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGG‑‑CCC‑‑CA‑C‑ACCC‑‑CCCCC‑‑CCCGTACGGTAATCGAGCTGCTGACCGCTg                                                 <  1:1847185/93‑1 (MQ=255)                                       tctGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGG‑‑CCC‑‑CA‑C‑A‑‑TCCCCCCC‑‑CCCGTACGGTAATCAAGCTGCCGAGCGCTGa                                                >  2:1508745/1‑93 (MQ=255)                                       tctGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGG‑‑CCC‑‑CA‑C‑A‑‑TCCCCCCC‑‑CCCGTACGGCAATCGAGccgctgaccgctga                                                >  1:1563163/1‑93 (MQ=255)                                        ctGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGG‑‑CCC‑‑CA‑C‑A‑‑TC‑CCCCC‑‑CCCGTACGGTAATCGAGCTGCTGACCGCTGata                                              <  2:208489/93‑1 (MQ=255)                                         tGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGG‑‑CCC‑‑CA‑C‑A‑‑TCCCCCCC‑‑CCCGTACGGTAATCGAGCTGCTGCCCGCTGata                                              >  2:1138526/1‑93 (MQ=255)                                         tGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGG‑‑CCC‑‑CA‑C‑A‑‑TCCCCCCC‑‑CCCGTACGGTAATCGAGCTGCTGACCGCTGata                                              <  2:482219/93‑1 (MQ=255)                                         tGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGG‑‑CCC‑‑CA‑C‑A‑‑TCCCCCCC‑‑CCCGTACGGTAATCGAGCTGCTGACCGCTGata                                              <  2:3721298/93‑1 (MQ=255)                                         tGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGG‑‑CCC‑‑CA‑C‑A‑‑TCCCCCCC‑‑CCCGCACGGTAATCGAGCTGCTGACCGCTGcta                                              >  1:1291810/1‑93 (MQ=255)                                          ggATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGATCTGG‑‑CCC‑‑CA‑C‑A‑‑TC‑CCCCC‑‑CCCGTACGGTAATCGAGCTGCTGACCGCTGATATc                                            >  1:3395671/1‑93 (MQ=255)                                          ggATTCGCCGCGACCTGCGCCAGCCTTGCTGCGCCCAGTGCTGG‑‑CCC‑‑CA‑C‑A‑‑TCCCCCCC‑‑CCCGTACGGTAATCGAGCTGCTGACCGCTGatat                                             <  2:23953/93‑1 (MQ=255)                                           gATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGG‑‑CCC‑‑CA‑C‑A‑‑TCCCCCCC‑‑CCCGTACGGTAATCGAGCTGCTGACCGCTGATATc                                            <  1:3749524/93‑1 (MQ=255)                                            aTTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGG‑‑CCC‑‑CA‑C‑A‑‑TCCCCCCC‑‑CCCGTACGGTAATCGAGCTGCTGACCGCTGATATcc                                           <  2:1297631/93‑1 (MQ=255)                                             ttCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGG‑‑CCC‑‑CA‑C‑A‑‑T‑‑CCCCC‑‑CCCGTACGGTAATCGAGCTGCTGACCGCTGATATCcccc                                        >  1:2568395/1‑93 (MQ=255)                                               cgccgcGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGG‑‑CCC‑‑CA‑C‑A‑‑TCCCCCCC‑‑CCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGcc                                        <  1:4179814/93‑1 (MQ=255)                                                ccctcgATCTGCCCCAGCCTTGCTGCGCCCAGGGCGGG‑‑CCC‑‑AA‑CAA‑‑C‑‑CCCCC‑‑CCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAg                                      <  2:1823428/89‑1 (MQ=255)                                                  cgcgATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGG‑‑CCC‑‑CA‑C‑A‑‑TCCCCCCC‑‑CCCGCAAGGGAAAAGAGCTGCGGAGCGCTGATATCCGCCAGc                                     >  1:204169/1‑93 (MQ=255)                                                   gcgATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGG‑‑CCC‑‑CA‑C‑A‑‑TC‑CCCCC‑‑CCCGTACGGTAATCGAGCTGCTGACCGCCGATATCCGCCAGCac                                   >  1:908214/1‑92 (MQ=255)                                                   ccgATCTGCCCCAGCCTTGCTGCGCCCAGTGCTGG‑‑CCC‑‑CA‑C‑ACCC‑‑CCCCC‑‑CCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCa                                    <  1:2681696/92‑1 (MQ=255)                                                    cgATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGC‑‑CCC‑‑CA‑C‑A‑‑TC‑CCCCC‑‑CCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATc                                  <  1:1545206/93‑1 (MQ=255)                                                    cgATCTGCCCCAGCCTTGCTGCGCCCAGTGCTGG‑‑CCC‑‑CA‑C‑ACCC‑‑CCCCC‑‑CCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCAt                                   <  1:2838061/93‑1 (MQ=255)                                                     gATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGG‑‑CCC‑‑CA‑C‑A‑‑TCCCCCCC‑‑CCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATc                                  <  2:3123817/93‑1 (MQ=255)                                                     gATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGG‑‑CCC‑‑CA‑C‑A‑‑TC‑CCCCC‑‑CCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCt                                 <  1:198414/93‑1 (MQ=255)                                                     gATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGG‑‑CCC‑‑CA‑C‑A‑‑TC‑CCCCC‑‑CCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCt                                 <  1:102524/93‑1 (MQ=255)                                                     gATCTGCCCCAGCCTTGCTGCGCCCAGTGCTGG‑‑CCC‑‑CA‑C‑A‑‑TCCCCCCC‑‑CCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATc                                  <  1:3149896/93‑1 (MQ=255)                                                     caTCTGCGCCAGCCTTGCTGCGCCCAGTGCTGG‑‑CCC‑‑CA‑C‑A‑‑TCCCCCCC‑‑CCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATc                                  <  2:3077393/92‑1 (MQ=255)                                                     caTCTGCGCCAGCCTTGCTGCGCCCAGTGCTGG‑‑CCC‑‑CA‑C‑A‑‑TC‑CCCCC‑‑CCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCt                                 <  1:737831/92‑1 (MQ=255)                                                       tCTGCGCCAGCCTTGCTGCGCCCAGTGCTGG‑‑CCC‑‑CA‑C‑A‑‑TCCCCCCC‑‑CCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTg                                <  1:286522/93‑1 (MQ=255)                                                         cccgcCAGCCTTCCTGCGCCAAGTGCGGGCCCCC‑‑CA‑C‑C‑‑C‑‑CCCCC‑‑CCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTTAc                              <  2:2635251/91‑1 (MQ=255)                                                          tccccAGCCTTGCTTGCCCCAGGGCCGG‑‑CCC‑‑CACC‑ACCC‑‑CCCCC‑‑CCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGAc                              <  2:4219366/90‑1 (MQ=255)                                                          gcgcCAGCCTTGCTGCGCCCAGTGCTGG‑‑CCC‑‑CA‑C‑A‑‑TCCCCCCC‑‑CCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACg                             >  1:1580054/1‑93 (MQ=255)                                                           cgcCAGCCTTGCTGCGCCCAGTGCTGG‑‑CCC‑‑CA‑C‑A‑‑TCCCCCCC‑‑CCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGc                            <  1:1070519/93‑1 (MQ=255)                                                           cgcCAGCCTTGCTGCGCCCAGTGCTGG‑‑CCC‑‑CA‑C‑A‑‑TCCCCCCC‑‑CCCCCACGGGAATCGAGCGCCTGACCCCCGCGATACGCCCCCATCTGCCGc                            >  2:3515739/1‑93 (MQ=255)                                                            gcCAGCCTTGCTGCGCCCAGTGCTGG‑‑CCC‑‑CA‑C‑A‑‑TCCCCCCC‑‑CCCGTACGGAAATCGGCCTGCTGACCGCTGAAATAGGCCAGCATCTGATGca                           >  1:1596988/1‑92 (MQ=255)                                                            gcCAGCCTTGCTGCGCCCAGTGCTGG‑‑CCC‑‑CA‑C‑A‑‑T‑‑CCCCCCGCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGcc                           >  1:4170890/1‑93 (MQ=255)                                                             ccAGCCTTGCTGCGCCCAGTGCTGG‑‑CCC‑‑CA‑C‑A‑‑TCCCCCCC‑‑CCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCa                          <  1:3613474/93‑1 (MQ=255)                                                             ccAGCCTCGCTGCGCCCAGTGCTGG‑‑CCC‑‑CA‑C‑A‑‑TCCCCCCC‑‑CCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCa                          <  1:1098060/93‑1 (MQ=255)                                                               aGCCTTGCTGCGCCCAGTGCTGG‑‑CCC‑‑CA‑C‑A‑‑TC‑CCCCC‑‑CCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCGGCATCTGACGCCAGTa                       >  2:760270/1‑93 (MQ=255)                                                                gCCTTGCTGCGCCCAGTGCTGG‑‑CCC‑‑CA‑C‑A‑‑TCCCCCCC‑‑CCCGTCCGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTa                       >  1:718014/1‑93 (MQ=255)                                                                 ccTTGCTGCGCCCAGTGCTGG‑‑CCC‑‑CA‑C‑A‑‑TCCCCCCC‑‑CCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTAc                      <  1:538422/93‑1 (MQ=255)                                                                 ccTTGCTGCGCCCAGTGCTGG‑‑CCC‑‑CA‑C‑A‑‑TCCCCCCC‑‑CCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTAc                      <  2:1069973/93‑1 (MQ=255)                                                                 ccTTGCCACGACCAGTGCTGG‑‑CCC‑‑CA‑C‑AACC‑‑CCCCC‑‑CCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTAACGCCAGTAc                      <  2:4615528/93‑1 (MQ=255)                                                                  cTTGCTGCGCCCAGGGCGGG‑‑CCC‑‑CA‑C‑ACCC‑‑CCCCC‑‑CCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACt                     <  2:1140639/93‑1 (MQ=255)                                                                     gctgcGCCCAGTGCTGG‑‑CCC‑‑CC‑C‑A‑‑TCCCCCCC‑‑CCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCAc                  <  1:2990636/93‑1 (MQ=255)                                                                      ctgcGCCCAGTGCTGG‑‑CCC‑‑CA‑C‑A‑‑TCCCCCCC‑‑CCCCCACGGTAATCCAGCTGCTGACCCCTCAGATCCGCCCGCCACTCAGGCCCGTACTCAcc                 >  1:293715/1‑92 (MQ=255)                                                                         cgcCCAGTGCTGG‑‑CCC‑‑CA‑C‑A‑‑TCCCCCCC‑‑CCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTAcg              <  2:2784358/93‑1 (MQ=255)                                                                          gcCCAGTGCTGG‑‑CCC‑‑CA‑C‑A‑‑TC‑CCCCC‑‑CCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGGCAGTACTCACTAcgcg            >  1:3100746/1‑93 (MQ=255)                                                                          ccccAGTGCTGG‑‑CCCCTCA‑C‑C‑‑C‑‑CCCCC‑‑CCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTAcgc             <  1:1910371/92‑1 (MQ=255)                                                                             cAGTGCTGG‑‑CCC‑‑CC‑C‑A‑‑TCCCCCCC‑‑CCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGcgcc          <  1:157796/93‑1 (MQ=255)                                                                             cAGTGCTGC‑‑CCC‑‑CA‑C‑A‑‑TCCCCCCC‑‑CCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGcgcc          <  2:2380942/93‑1 (MQ=255)                                                                              cgTGCTGG‑‑CCC‑‑CG‑C‑A‑‑TCCCCCCC‑‑CCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGcgccc         <  1:1529304/92‑1 (MQ=255)                                                                              aGTGCTGG‑‑CCC‑‑CA‑C‑A‑‑TCCCCCCC‑‑CCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGcgccc         <  2:1814309/93‑1 (MQ=255)                                                                               gTGCTGG‑‑CCC‑‑CA‑C‑A‑‑TCCCCCCC‑‑CCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGcgcccc        <  1:2131747/93‑1 (MQ=255)                                                                               gTGCTGG‑‑CCC‑‑CA‑C‑A‑‑T‑‑CCCCCCTCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGcgcccc        <  1:179144/93‑1 (MQ=255)                                                                               cggCTGG‑‑CCC‑‑CA‑C‑A‑‑TCCCCCCC‑‑CCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGcgcccc        <  1:1290831/91‑1 (MQ=255)                                                                                tGCTGG‑‑CCC‑‑CA‑C‑A‑‑TCCCCCCC‑‑CCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGcgccccc       >  1:753293/1‑92 (MQ=255)                                                                                  cTGG‑‑CCC‑‑CA‑C‑ACCC‑‑CCCCC‑‑CCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGcgccccgcc     <  1:3163811/93‑1 (MQ=255)                                                                                   tGG‑‑CCC‑‑CA‑C‑A‑‑TCCCCCCC‑‑CCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGcgccccgccc    <  1:2090075/93‑1 (MQ=255)                                                                                    gg‑‑CCC‑‑CA‑C‑AC‑C‑‑CCCCC‑‑CCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGccccc  <  1:4224939/93‑1 (MQ=255)                                                                                                      |                                                                                      CTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGG‑‑CCC‑‑CA‑C‑A‑‑T‑‑CCCCC‑‑CCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCC  >  NC_000913/3725647‑3725821

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑