Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,899,957 (C)5→6 coding (1373/1413 nt) bglB ← phospho‑beta‑glucosidase B

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,899,9521.C99.6% 765.7 / NA 247L460V (CTG→GTG) bglBphospho‑beta‑glucosidase B
Reads supporting (aligned to +/- strand):  ref base . (1/0);  major base C (114/131);  minor base A (1/0);  total (116/131)
Fisher's exact test for biased strand distribution p-value = 4.67e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 273 total aligned reads displayed.

ATGGTGCAAAGAAAGAATTAACGCATCGCATCCAGACTGTTCTGAATGCGACGATAATTAAGGTGCTTTAATGGTTA‑TTTTTTTTAATGACAG‑C‑CCCCGCGTCTTGATCACCTCTGCATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGCTTCCTTCGCCATTATCATCACGATC  >  NC_000913/3899860‑3900039
                                                                                              |                                                                                        
aTGGTGCAAAGAAAGAATTAACGCATCGCATCCAGACTGTTCTGAATGCGACGATAATTAAGGTGCTTTAATGGTTA‑TTTTTTTTAATGACAg                                                                                           >  1:3734793/1‑93 (MQ=255)
 tGGTGCAAAGAAAGAATTAACGCATCGCATCCAGACTGTTCTGAATGCGACGATAATTAAGGTGCTTTAATGGTTA‑TTTTTTTTAATGACAG‑c                                                                                         >  2:4468275/1‑93 (MQ=255)
   gTGCAAAGAAAGAATTAACGCATCGCATCCAGACTGTTCTGAATGCGACGATAATTAAGGTGCTTTAATGGTTATTTTTTTTTAATGACAG‑c‑c                                                                                       <  1:1044472/93‑1 (MQ=255)
   gTGCAAAGAAAGAATTAACGCATCGCATCCAGACTGTTCTGAATGCGACGATAATTAAGGTGCTTTAATGGTTA‑TTTTTTTTAATGACAG‑c‑cc                                                                                      <  1:4093037/93‑1 (MQ=255)
    tGCAAAGAAAGAATTAACGCATCGCATCCAGACTGTTCTGAATGCGACGATAATTAAGGTGCTTTAATGGTTA‑TTTTTTTTAATGACAG‑c‑ccc                                                                                     >  2:3851203/1‑93 (MQ=255)
     gCAAAGAAAGAATTAACGCATCGCATCCAGACTGTTCTGAATGCGACGATAATTAAGGTGCTTTAATGGTTA‑TTTTTTTTAATGACAG‑c‑cccc                                                                                    >  1:868127/1‑93 (MQ=255)
        aagaaagaATTAACGCATCGCATCCAGACTGTTCTGAATGCGACGATAATTAAGGTGCTTTAATGGTTA‑TTTTTTTTAATGACAG‑c‑cccccgc                                                                                 >  1:3895456/1‑90 (MQ=255)
           aaagaaTTAACGCATCGCATCCAGACTGTTCTGAATGCGACGATAATTAAGGTGCTTTAATGGTTA‑TTTTTTTTAATGACAGCC‑CCCCGCGTc                                                                               <  1:717988/93‑1 (MQ=255)
           aaagaaTTAACGCATCGCATCCAGACTGTTCTGAATGCGACGATAATTAAGGTGCTTTAATGGTTA‑TTTTTTTTAATGACAGCC‑CCCCGCGTc                                                                               <  1:2745191/93‑1 (MQ=255)
            aagaaTTAACGCATCGCATCCAGACTGTTCTGAATGCGACGATAATTAAGGTGCTTTAATGGTTA‑TTTTTTTTAATGACAGCC‑CCCCGCGTCt                                                                              <  1:3355156/93‑1 (MQ=255)
             agaaTTAACGCATCGCATCCAGACTGTTCTGAATGCGACGATAATTAAGGTGCTTTAATGGTTA‑TTTTTTTTAATGACAGCC‑CCCCGCGTCtt                                                                             >  2:1087079/1‑93 (MQ=255)
                aTTAACGCATCGCATCCAGACTGTTCTGAATGCGACGATAATTAAGGTGCTTTAATGGTTA‑TTTTTTTTAATGACAGCC‑CCCCGCGTCTTGAt                                                                          >  1:955582/1‑93 (MQ=255)
                aTTAACGCATCGCATCCAGACTGTTCTGAATGCGACGATAATTAAGGTGCTTTAATGGTTA‑TTTTTTTTAATGACAGCC‑CCCCGCGTCTTGAt                                                                          <  1:2168339/93‑1 (MQ=255)
                 ttAACGCATCGCATCCAGACTGTTCTGAATGCGACGATAATTAAGGTGCTTTAATGGTTA‑TTTTTTTTAATGACAGCC‑CCCCGCGTCTTGATc                                                                         <  1:2345369/93‑1 (MQ=255)
                 ttAACGCATCGCATCCAGACTGTTCTGAATGCGACGATAATTAAGGTGCTTTAATGGTTA‑TTTTTTTTAATGACAGCC‑CCCCGCGTCTTGATc                                                                         <  2:4577442/93‑1 (MQ=255)
                 ttAACGCATCGCATCCAGACTGTTCTGAATGCGACGATAATTAAGGTGCTTTAATGGTTA‑TTTTTTTTAATGACAGAC‑CCCCGCGTCTTGATc                                                                         >  2:372632/1‑93 (MQ=255)
                    aCGCATCGCATCCAGACTGTTCTGAATGCGACGATAATTAAGGTGCTTTAATGGTTA‑TTTTTTTTAATGACAGCC‑CCCCGCGTCTTGATCAcc                                                                      >  2:299594/1‑93 (MQ=255)
                    aCGCATCGCATCCAGACTGTTCTGAATGCGACGATAATCAAGGTGCTTTAATGGTTA‑TTTTTTTTAATGACAGCC‑CCCCGCGTCTTGATGAcc                                                                      <  2:4132984/93‑1 (MQ=255)
                         tcgcatcCAGACTGTTCTGAATGCGACGATAATTAAGGTGCTTTAATGGTTA‑TTTTTTTTAATGACAGCC‑CCCCGCGTCTTGATCACctctac                                                                 >  2:1984612/1‑91 (MQ=255)
                         tcgcatcCAGACTGTTCTGAATGCGACGATAATTAAGGTGCTTTAATGGTTA‑TTTTTTTTAATGACAGCC‑CCCCGCGTCTTGATCACctctac                                                                 <  2:4437492/93‑3 (MQ=255)
                         tcgcatcCAGACTGTTCTGAATGCGACGATAATTAAGGTGCTTTAATGGTTA‑TTTTTTTTAATGACAGCC‑CCCCGCGTCGTGATCACctctac                                                                 <  2:4606044/93‑3 (MQ=255)
                          cgcatcCAGACTGTTCTGAATGCGACGATAATTAAGGTGCTTTAATGGTTA‑TTTTTTTTAATGACAGCC‑CCCCGCGTCTTGATCCCCTCTACa                                                                <  2:3220065/93‑1 (MQ=255)
                          cgcatcCAGACTGTTCTGAATGCGACGATAATTAAGGTGCTTTAATGGTTA‑TTTTTTTTAATGACAGCC‑CCCCGCGTCTTGATCACCTCTACa                                                                <  1:739464/93‑1 (MQ=255)
                           gcatcCAGACTGTTCTGAATGCGACGATAATTAAGGTGCTTTAATGGTTA‑TTTTTTTTAATGACAGCC‑CCCCGCGTCTTGATCACCTCTACAt                                                               <  2:1587424/93‑1 (MQ=255)
                              tcCAGACTGTTCTGAATGCGACGATAATTAAGGTGCTTTAATGGTTA‑TTTTTTTTAATGACAGCC‑CCCCGCGTCTTGATCACCTCTACATAcc                                                            <  1:2116684/93‑1 (MQ=255)
                              tcCAGACTGTTCTGAATGCGACGATAATTAAGGTGCTTTAATGGTTA‑TTTTTTTTAATGACAG‑CACCCCGCGTCTTGATCACCTCTACATAcc                                                            >  2:2706434/1‑93 (MQ=255)
                                cAGACTGTTCTGAATGCGACGATAATTAAGGTGCTTTAATGGTTA‑TTTTTTTTAATGACAGCC‑CCCCGCGTCTTGATCACCTCTACATACCAt                                                          >  2:2571951/1‑93 (MQ=255)
                                cAGACTGTTCTGAATGCGACCATAATTAAGGTGCTTTAATGGTTA‑TTTTTTTTAATGACAGCC‑CCCCGCGTCTTGATCACCTCTACATACCAt                                                          <  2:4216438/93‑1 (MQ=255)
                                 aGACTGTTCTGAATGCGACGATAATTAAGGTGCTTTAATGGTTA‑TTTTTTTTAATGACAGCC‑CCCCGCGTCTTGATCACCTCTACATACCATc                                                         >  2:1542191/1‑93 (MQ=255)
                                  gACTGTTCTGAATGCGACGATAATTAAGGTGCTTTAATGGTTA‑TTTTTTTTAATGACAGCC‑CCCCGCGTCTTGATCACCTCTACATACCATcc                                                        >  1:1189871/1‑93 (MQ=255)
                                    cTGTTCTGAATGCGACGATAATTAAGGTGCTTTAATGGTTA‑TTTTTTTTAATGACAGCC‑CCCCGCGTCTTGATCACCTCTACATACCATCCGa                                                      >  1:2151114/1‑93 (MQ=255)
                                     tGTTCTGAATGCGACGATAATTAAGGTGCTTTAATGGTTA‑TTTTTTTTAATGACAGCC‑CCCCGCGTCTTGATCACCTCTACATACCATCCGaa                                                     <  1:1665871/93‑1 (MQ=255)
                                     tGTTCTGAATGCGACGATAATTAAGGTGCTTTAATGGTTA‑TTTTTTTTAATGACAGCC‑CCCCGCGTCTTGATCACCTCTACATACCATCCGaa                                                     >  1:2297562/1‑93 (MQ=255)
                                      gTTCTGAATGCGCCGATAATTAAGGTGCTTTAATGGTTA‑TTTTTTTTAATGACAGCC‑CCCCGCGTCTTGATCACCTCTACATACCATCCGAAg                                                    <  2:2127244/93‑1 (MQ=255)
                                       ttCTGAATGCGACGATAATTAAGGTGCTTTAATGGTTA‑TTTTTTTTAATGACAGCC‑CCCCGCGTCTTGATCACCTCTACATACCATCCGAAGc                                                   <  1:4650627/93‑1 (MQ=255)
                                        tCTGAATGCGACGATAATTAAGGTGCTTTAATGGTTATTTTTTTTTAATGACAGCC‑CCCCGCGTCTTGATCACCTCTACATACCATCCGAAGc                                                   >  2:434347/1‑93 (MQ=255)
                                          tGAATGCGACGATAATTAAGGTGCTTTAATGGTTA‑TTTTTTTTAATGACAGCC‑CCCCGCGTCTTGATCACCTCTACATACCATCCGAAGCttt                                                <  2:1403260/93‑1 (MQ=255)
                                          tGAATGCGACGATAATTAAGGTGCTTTAATGGTTA‑TTTTTTTTAATGACAGCC‑CCCCGCGTCCTTGTCACCCCTCCATACCATCCCAAACttt                                                >  2:866895/1‑93 (MQ=255)
                                            aaTGCGACGATAATTAAGGTGCTTTAATGGTTA‑TTTTTTTTAATGACAGCC‑CCCCGCGTCTTGATCACCTCTACATACCATCCGAAGCTTTTc                                              <  1:4155416/93‑1 (MQ=255)
                                            aaTGCGACGATAATTAAGGTGCTTTAATGGTTA‑TTTTTTTTAATGACAGCC‑CCCCGCGTCTTGATCACCTCTACATACCATCCGAAGCTTTTc                                              >  1:2389711/1‑93 (MQ=255)
                                             aTGCGACGATAATTAAGGTGCTTTAATGGTTA‑TTTTTTTTAATGACAGCC‑CCCCGCGTCTTGATCACCTCTACATACCATCCGAAGCTTTTCt                                             <  2:1897395/93‑1 (MQ=255)
                                              tGCGACGATAATTAAGGTGCTTTAATGGTTA‑TTTTTTTTAATGACAGCC‑CCCCGCGTCTTGATCACCTCTACATACCATCCGAAGCTTTTCtt                                            <  1:3970843/93‑1 (MQ=255)
                                                 gacgaTAATTAAGGTGCTTTAATGGTTA‑TTTTTTTTAATGACAGCC‑CCCCGCGTCTTGATCACCTCTACATACCATCCGAAGCTTTTCTTGCg                                         >  1:3088452/1‑93 (MQ=255)
                                                 gacgaTAATTAAGGTGCTTTAATGGTTA‑TTTTTTTTAATGACAGCC‑CCCCGCGTCTTGATCACCTCTACATACCATCCGAAGCTTTTCCTGCg                                         >  2:4147519/1‑93 (MQ=255)
                                                  acgaTAATTAAGGTGCTTTAATGGTTA‑TTTTTTTTAATGACAGCC‑CCCCGCGTCTTGATCACCTCTACATACCATCCGAAGCTTTTCTTGCgt                                        <  1:2572053/93‑1 (MQ=255)
                                                  acgaTAATTAAGGTGCTTTAATGGTTA‑TTTTTTTTAATGACAGCC‑CCCCGCGTCTTGATCACCTCTACATACCATCCGAAGCTTTTCTTGCgt                                        <  1:2720593/93‑1 (MQ=255)
                                                    gaTAATTAAGGTGCTTTAATGGTTA‑TTTTTTTTAATGACAGCC‑CCCCGCGTCTTGATCACCTCTACATACCATCCGAAGCTTTTCTTGCgtgt                                      <  2:3689286/93‑1 (MQ=255)
                                                    gaTAATTAAGGTGCTTTAATGGTTA‑TTTTTTTTAATGACAGCC‑CCCCGCGTCTTGATCACCTCTACATACCATCCGAAGCTTTTCTTGCgtgt                                      >  2:3650896/1‑93 (MQ=255)
                                                    gaTAATTAAGGTGCTTTAATGGTTA‑TTTTTTTTAATGACAGCC‑CCCCGCGTCTTGATCACCTCTACATAACATCCGAAGCTTTTCTTGCgtgt                                      >  1:2473415/1‑93 (MQ=255)
                                                     aTAATTAAGGTGCTTTAATGGTTA‑TTTTTTTTAATGACAGCC‑CCCCGCGTCTTGATCACCTCTACATACCATCCGAAGCTTTTCTTGCGTGtt                                     >  2:3115525/1‑93 (MQ=255)
                                                     aTAATTAAGGTGCTTTAATGGTTA‑TTTTTTTTAATGACAGCC‑CCCCGCGTCTTGATCACCTCTACATACCATCCGAAGCTTTTCTTGCGTGtt                                     >  2:3326156/1‑93 (MQ=255)
                                                      tAATTAAGGTGCTTTAATGGTTA‑TTTTTTTTAATGACAGCC‑CCCCGCGTCTTGATCACCTCTACATACCATCCGAAGCTTTTCTTGCGTGTTc                                    >  1:4417114/1‑93 (MQ=255)
                                                      tAATTAAGGTGCTTTAATGGTTA‑TTTTTTTTAATGACAGCC‑CCCCGCGTCTTGATCACCTCTACATACCATCCGAAGCTTTTCTTGCGTGTTc                                    <  2:1370884/93‑1 (MQ=255)
                                                      tAATTAAGGGGCTTTAATGGTTA‑TTTTTTTTAATGACAGCC‑CCCCGCGTCTTGATCACCTCTACATACCATCCGAAGATTTTCTTGCGTGTTc                                    >  1:2070209/1‑93 (MQ=255)
                                                       aaTTAAGGTGCTTTAATGGTTA‑TTTTTTTTAATGACAGCC‑CCCCGCGTCTTGATCACCTCTACATACCATCCGAAGCTTTTCTTGCGTGTTCt                                   >  1:1313158/1‑93 (MQ=255)
                                                       aaTTAAGGTGCTTTAATGGTTA‑TTTTTTTTAATGACAGCC‑CCCCGCGTCTTGATCACCTCTACATACCATCCGAAGCTTTTCTTGCGTGTTCt                                   <  1:356517/93‑1 (MQ=255)
                                                       aaTTAAGGTGCTTTAATGGTTA‑TTTTTTTTAATGACAGCC‑CCCCGCGTCTTGATCACCTCTACATACCATCCGAAGCTTTTCTTGCGTGTTCt                                   <  2:2650330/93‑1 (MQ=255)
                                                       aaTTAAGGTGCTTTAATGGTTA‑TTTTTTTTAATGACAGCC‑CCCCGCGTCTTGATCACCTCTACATAACATCCGAAGCTTTTCTTGCGTGTTCt                                   >  1:1619398/1‑93 (MQ=255)
                                                        aTTAAGGTGCTTTAATGGTTA‑TTTTTTTTAATGACAGCC‑CCCCGCGTCTTGATCACCTCTACATACCATCCGAAGCTTTTCTTGCGTGTTCtt                                  <  2:1032926/93‑1 (MQ=255)
                                                         ttAAGGTGCTTTAATGGTTA‑TTTTTTTTAATGACAGCC‑CCCCGCGTCTTGATCACCTCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTtg                                 <  2:2820495/93‑1 (MQ=255)
                                                         ttAAGGTGCTTTAATGGTTA‑TTTTTTTTAATGACAGCC‑CCCCGCGTCTTGATCACCTCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTtg                                 <  1:41703/93‑1 (MQ=255)
                                                         ttAAGGTGCTTTAATGGTTA‑TTTTTTTTAATGACAGCC‑CCCCGCGTCTTGATCACCTCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTtg                                 <  1:55942/93‑1 (MQ=255)
                                                           aaGGTGCTTTAATGGTTA‑TTTTTTTTAATGACAGCC‑CCCCGCGTCTTGATCACCTCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTtgtg                               <  2:1461615/93‑1 (MQ=255)
                                                            aGGTGCTTTAATGGTTA‑TTTTTTTTAATGACAGCC‑CCCCGCGTCTTGATCACCTCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTtgtgg                              >  2:1654547/1‑92 (MQ=255)
                                                            aGGTGCTTTAATGGTTA‑TTTTTTTTAATGACAGCC‑CCCCGCGTCTTGATCACCTCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGa                              >  1:3523057/1‑93 (MQ=255)
                                                             ggTGCTTTAATGGTTA‑TTTTTTTTAATGACAGCC‑CCCCGCGTCTTGATCACCTCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAg                             >  2:2206073/1‑93 (MQ=255)
                                                               tGCTTTAATGGTTA‑TTTTTTTTAATGACAGCC‑CCCCGCGTCTTGATCACCTCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGc                           >  2:1926979/1‑93 (MQ=255)
                                                                gCTTTAATGGTTA‑TTTTTTTTAATGACAGCC‑CCCCGCGTCTTGATCACCTCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGct                          >  2:496860/1‑93 (MQ=255)
                                                                gCTTTAATGGTTA‑TTTTTTTTAATGACAGCC‑CCCCGCGCCTTGATTACCTCTACATACCATCCGAAGCTTTTTTTGCGTGTTCTTGTGAGGct                          >  2:3283563/1‑93 (MQ=255)
                                                                  tttAATTGTTA‑TTTTTTTTAATGACAGCC‑CCCCGCGTCTTGATCCCCTCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGcttc                        <  1:1003761/93‑1 (MQ=255)
                                                                  tttAATGGTTA‑TTTTTTTTAATGACAGCC‑CCCCGCGTCTTGATCACCTCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGcttc                        >  1:788170/1‑93 (MQ=255)
                                                                   ttAATGGTTA‑TTTTTTTTAATGACAGCC‑CCCCGCGTCTTGATCACCTCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGcttcc                       <  2:2053619/93‑1 (MQ=255)
                                                                     taTGGTTA‑TTTTTTTTAATGACAGCC‑CCCCGCGTCTTGATCACCTCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGcttcctt                     <  1:461197/92‑1 (MQ=255)
                                                                       tGGTTA‑TTTTTTTTAATGACAGCC‑CCCCGCGTCTTGCTCACCTCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGCTTCCTTCg                   >  2:769766/1‑93 (MQ=255)
                                                                        ggTTA‑TTTTTTTTAATGACAGCC‑CCCCGCGTCTTGATCACCTCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGTGGCTTCCTTCGc                  >  1:4297172/1‑93 (MQ=255)
                                                                        ggTTA‑TTTTTTTTAATGACAGCC‑CCCCGCGTCTTGATCACCTCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGCTTCCTTCGc                  >  1:4540985/1‑93 (MQ=255)
                                                                        ggTTA‑TTTTTTTTAATGACAGCC‑CCCCGCGTCTTGATCACCTCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGCTTCCTTCGc                  >  2:3565208/1‑93 (MQ=255)
                                                                         gTTA‑TTTTTTTTAATGACAGCC‑CCCCGCGTCTTGATCACCTCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGCTTCCTTCGcc                 <  1:1407534/93‑1 (MQ=255)
                                                                          ttA‑TTTTTTTTAATGACAGCC‑CCCCGCGTCTTGATCACCTCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGCTTCCTTCGCCa                >  2:3390571/1‑93 (MQ=255)
                                                                          ttA‑TTTTTTTTAATGACAGCC‑CCCCGCGTCTTGATCACCTCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGCTTCCTTCGCCa                >  1:1357019/1‑93 (MQ=255)
                                                                          ttA‑TTTTTTTTAATGACAGCC‑CCCCGCGTCTTGATCACCTCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGCTTCCTTCGCCa                <  2:4020417/93‑1 (MQ=255)
                                                                          ttA‑TTTTTTTTAATGACAGCC‑CCCCGCGTCTTGATCACCTCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGCTTCCTTCGCCa                <  2:3858187/93‑1 (MQ=255)
                                                                            a‑TTTTTTTTAATGACAGCC‑CCCCGCGTCTTGATCACCTCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGCTTCCTTCGCCAtt              <  2:3099523/93‑1 (MQ=255)
                                                                            a‑TTTTTTTTAATGACAGCC‑CCCCGCGTCTTGATCACCTCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGCTTCCTTCGCCAtt              >  2:1216481/1‑93 (MQ=255)
                                                                            a‑TTTTTTTTAATGACAGCC‑CCCCGCGTCTTGATCACCTCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGCTTCCTTCGCCAtt              <  2:3494967/93‑1 (MQ=255)
                                                                            a‑TTTTTTTTAATGACAGCC‑CCCCGCGTCTTGATCACCTCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGCTTCCTTCGCCAtt              >  1:1628705/1‑93 (MQ=255)
                                                                              ttttttttAATGACAGCC‑CCCCGCGTCTTGATCACCTCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGCTTCCTTCGCCATTa             <  2:1712964/93‑1 (MQ=255)
                                                                               tttttttAATGACAGCC‑CCCCGCGTCTTGATCACCTCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGCTTCCTTCGCCATTat            <  1:3875543/93‑1 (MQ=255)
                                                                               tttttttAATGACAGCC‑CCCCGCGTCTTGATCACCTCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGCTTCCTTCGCCATTat            <  1:1767476/93‑1 (MQ=255)
                                                                                ttttttAATGACAGCC‑CCCCGCGTCTTGATCACCTCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGCTTCCTTCGCCATTatc           <  1:3039535/93‑1 (MQ=255)
                                                                                  ttttAATGACAGCC‑CCCCGCGTCTTGATCACCTCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGCTTCCTTCGCCATTatcat         <  1:3156492/93‑1 (MQ=255)
                                                                                    ttAATGACAGCC‑CCCCGCGTCTTGATCACCTCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGCTTCCTTCTCCATTAtcatca       >  2:1281559/1‑93 (MQ=255)
                                                                                    ttAATGACAGCC‑CCCCGCGTCTTGATCACCTCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGCTTCCTTCGCCATTAtcatca       <  2:2416575/93‑1 (MQ=255)
                                                                                    ttAATGACAGCC‑CCCCGCGTCTTGATCACCTCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGCTTCCTTCGCCATTAtcatca       >  2:582912/1‑93 (MQ=255)
                                                                                    ttAATGACAGCC‑CCCCGCGTCTTGATCACCTCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGCTTCCTTCGCCATTAtcatca       >  1:3654593/1‑93 (MQ=255)
                                                                                     tAATGACAGCC‑CCCCGCGTCTTGATCACCTCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGCTTCCTTCGCCATTATCATCAc      >  1:485158/1‑93 (MQ=255)
                                                                                      aaTGACAGCC‑CCCCGCGTCTTGATCACCTCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGCTTCCTTCGCCATTATCATCACg     <  1:2835904/93‑1 (MQ=255)
                                                                                      aaTGACAGCC‑CCCCGCGTCTTGATCACCTCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGCTTCCTTCGCCATTATCATCACg     >  1:1956762/1‑93 (MQ=255)
                                                                                      aaTGACAGCC‑CCCCGCGTCTTGATCACCTCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGCTTCCTTCGCCATTATCATCACg     >  1:1503407/1‑93 (MQ=255)
                                                                                       aTGACAGCC‑CCCCGCGTCTTGATCACCTCTACATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGCTTCCTTCGCCATTATCATCACGa    <  1:2367261/93‑1 (MQ=255)
                                                                                              |                                                                                        
ATGGTGCAAAGAAAGAATTAACGCATCGCATCCAGACTGTTCTGAATGCGACGATAATTAAGGTGCTTTAATGGTTA‑TTTTTTTTAATGACAG‑C‑CCCCGCGTCTTGATCACCTCTGCATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGCTTCCTTCGCCATTATCATCACGATC  >  NC_000913/3899860‑3900039

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑