BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,980,297	(C)_8→9	noncoding (79/87 nt)	leuT →	tRNA‑Leu

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,980,289	1	.	C	89.1%	429.8 / 49.0	156	noncoding (71/87 nt)	leuT	tRNA‑Leu
Reads supporting (aligned to +/- strand): ref base . (4/13); new base C (42/97); total (46/110)
Fisher's exact test for biased strand distribution p-value = 7.79e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 202 total aligned reads displayed.

CATTATTAGAAGTTGTGACAATGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCC‑TTACGGACGTGGGGGTTCAAG‑T‑‑C‑CCC‑CCCCTCGCACCACGACTTTAAAG‑AATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAGCGCAGCTTGGTAGCGCAAC  >  NC_000913/3980197‑3980376

cATTATTAGAAGTTGTGACAATGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCC‑TTACGGACGTGGGGGTTCAAG‑t                                                                                              <  2:4393461/93‑1 (MQ=255)

cATTATTAAAAGTTGTGACAATACGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCC‑TTACGGACGTGGGGGTTCAAG‑t                                                                                              <  2:2882033/93‑1 (MQ=255)

 attattAGAAGTTGTGACAATGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCC‑TTACGGACGTGGGGGTTCAAG‑T‑‑c                                                                                           >  1:1134376/1‑93 (MQ=255)

 attattAGAAGTTGTGACAATGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCC‑TTACGGACGTGGGGGTTCAAG‑T‑‑c                                                                                           >  1:3640039/1‑93 (MQ=255)

  ttattaGAAGTTGTGACAATGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCC‑TTACGGACGTGGGGGGTCAAG‑T‑‑c‑c                                                                                         >  2:4306035/1‑93 (MQ=255)

   tattaGAAGTTGTGACAATGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCC‑TTACGGACGTGGGGGTTCAAG‑T‑‑c‑cc                                                                                        >  2:3054562/1‑93 (MQ=255)

     ttaGAAGTTGTGACAATGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCC‑TTACGGACGTGGGGGTTCAAG‑T‑‑c‑ccc‑c                                                                                     >  2:2052006/1‑93 (MQ=255)

     ttaGAAGTTGTGACAATGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCC‑TTACGGACGTGGGGGTTCAAG‑T‑‑c‑ccc‑c                                                                                     >  1:2215973/1‑93 (MQ=255)

       aGAAGTTGTGACAATGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCC‑TTACGGACGTGGGGGTTCAAG‑T‑‑c‑ccc‑ccc                                                                                   >  2:1040558/1‑93 (MQ=255)

        gAAGTTGTGACAATGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCC‑TTACGGACGTGGGGGGTCAAG‑C‑‑c‑ccc‑cccc                                                                                  >  2:3663035/1‑93 (MQ=255)

         aaGTTGTGACAATGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCC‑TTACGGACGTGGGGGTTCAAG‑T‑‑c‑gcc‑ccccc                                                                                 >  1:188325/1‑92 (MQ=255)

         aaGTTGTGACAATGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCC‑TTACGGACGTGGGGGTTCAAG‑T‑‑c‑ccc‑ccccc                                                                                 <  1:633616/93‑2 (MQ=255)

          aGTTGTGACAATGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCC‑TTACGGACGTGGGGGTTCAAA‑T‑‑c‑ccc‑ccccct                                                                                >  1:3391415/1‑91 (MQ=255)

           gTTGTGACAATGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCC‑TTACGGACGTGGGGGTTCAAG‑T‑‑c‑ccc‑ccccctc                                                                               >  1:4158779/1‑90 (MQ=255)

           gTTGTGACAATGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCC‑TTACGGACGTGGGGGGTTCAG‑T‑‑c‑gcc‑ccccccc                                                                               >  2:866720/1‑90 (MQ=14)

           attGTGACAATGCGAAGGTGCCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCC‑TTACGGACGTGGGGGTTCAAG‑T‑‑c‑ccc‑ccccctc                                                                               <  1:3920314/92‑4 (MQ=255)

                  cAATGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCC‑TTACGGACGTGGGGGTTCAAG‑TC‑C‑CCC‑CCCCTCCcaccac                                                                         >  2:1849711/1‑93 (MQ=255)

                  cAATGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCC‑TTACGGACGTGGGGGGTCAAG‑TC‑C‑CCC‑CCCCTCGcaccac                                                                         >  1:4023163/1‑93 (MQ=255)

                    aTGCGAAGGTGGCGGAATTGGTAGACGCTCTAGCTTCAGGTGTTAGTGTCC‑TTACGGACGTGGGGGTTCAAG‑TC‑C‑CCC‑CCCCTCGCACCACGa                                                                       <  1:442183/93‑1 (MQ=255)

                    aTGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCC‑TTACGGACGTGGGGGTTCAAG‑TC‑C‑CCC‑CCCCTCCCACCACgc                                                                       >  1:1542513/1‑92 (MQ=11)

                     tGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCC‑TTACGGACGTGGGGGTTCAAG‑TC‑C‑CCC‑CCCCTCGCACCACGAc                                                                      <  1:1585515/93‑1 (MQ=255)

                     tGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCC‑TTACGGACGTGGGGGTTCAAG‑TC‑C‑CCC‑CCCCTCGCACCACGAc                                                                      <  2:3412818/93‑1 (MQ=255)

                     tGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCC‑TTACGGACGTGGGGGTTCAAG‑TC‑C‑CCC‑CCCCTCGCACCACGAc                                                                      <  2:1783512/93‑1 (MQ=255)

                         aaGGTGGCGGAATGGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCC‑TTACGGACGTGGGGGTTCAAG‑TC‑C‑CCC‑CCCCTCGCACCACGACTTTa                                                                  <  2:4043615/93‑1 (MQ=255)

                          aGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCC‑TTACGGACGTGGGGGTTCAAG‑TC‑C‑CCC‑CCCCTCGCACCACGACTTTaa                                                                 >  2:3618974/1‑93 (MQ=255)

                           ggcggCGGAATGGGTAGGCGCGCCAGGTTCCGGTGTTTGTGTCC‑TTACGGACGTGGGGGTTCAAG‑TC‑C‑CCC‑CCCCTCGCACCACGACTTTaaa                                                                <  2:78021/90‑1 (MQ=14)

                           ggTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCC‑TTACGGACGTGGGGGTTCAAG‑TC‑C‑CCC‑CCCCTCGCACCACGACTTTaaa                                                                <  2:4270468/93‑1 (MQ=255)

                           ggTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCC‑TTACGGACGTGGGGGTTCAAG‑TC‑C‑CCC‑CCCCTCGCACCACGACTTTaaa                                                                <  1:2155763/93‑1 (MQ=255)

                           ggTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCC‑TTACGGACGTGGGGGTTCAAG‑TC‑C‑CCC‑CCCCTCGCACCACGACTTTaaa                                                                <  2:2557725/93‑1 (MQ=255)

                           ggTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCC‑TTACGGACGTGGGGGTTCAAG‑TC‑C‑CCC‑CCCCTCGCACCACGACTTTaaa                                                                <  1:4199348/93‑1 (MQ=255)

                              ggCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCC‑TTACGGACGTGGGGGTTCAAG‑TC‑C‑CCC‑CCCCTCGCACCACGACTTTAAAG‑aa                                                            <  1:4114913/93‑1 (MQ=255)

                               gCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCC‑TTACGGACGTGGGGGTTCAAG‑TC‑C‑CCC‑CCCCTCGCACCACGACTTTAAAG‑AAt                                                           >  2:3520810/1‑93 (MQ=255)

                                cGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCC‑TTACGGACGTGGGGGTTCAAG‑TC‑C‑CCC‑CCCCTCGCACCACGACTTTAAAG‑AAtt                                                          <  1:224311/93‑1 (MQ=255)

                                cGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCC‑TTACGGACGTGGGGGTTCAAG‑TC‑C‑CCC‑CCCCTCGCACCACGACTTTAAAG‑AAtt                                                          <  1:3663882/93‑1 (MQ=255)

                                cGGAATTGGTAGACGCGCTAGCTTCAGGAGTTAGTGTCC‑TTACGGACGTGGGGGTTCAAG‑TC‑C‑CCC‑CCCCTCGCACCACGACTTTAAAG‑AAtt                                                          <  1:4510495/93‑1 (MQ=255)

                                  gAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCC‑TTACGGACGTGGGGGTTCAAG‑TC‑C‑CCC‑CCCCTCGCACCACGACTTTAAAG‑AATTGa                                                        <  2:621531/93‑1 (MQ=255)

                                   aaTTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCC‑TTACGGACGTGGGGGTTCAAG‑TC‑C‑CCC‑CCCCTCGCACCACGACTTTAAAG‑AATTGaa                                                       >  2:3853407/1‑93 (MQ=255)

                                   aaTTGGTAGACGCGCTAGCTTCAGGCGTTAGTGTCC‑TTACGGACGTGGGGGTTCAAGTT‑‑C‑CCC‑CCCCTCGCACCACGACTTTAAAG‑AATTCaa                                                       >  1:4398586/1‑93 (MQ=17)

                                    aTTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCC‑TTACGGACGTGGGGGTTCAAG‑TC‑C‑CCC‑CCCCTCGCACCACGACTTTAAAG‑AATTGAAc                                                      >  2:2737507/1‑93 (MQ=255)

                                     ttGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCC‑TTACGGACGTGGGGGTTCAAG‑TC‑C‑CCC‑CCCCTCGCACCACGACTTTAAAG‑AATTGAACt                                                     <  2:2592402/93‑1 (MQ=255)

                                      tGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCC‑TTACGGACGTGGGGGTTCAAG‑TC‑C‑CCC‑CCCCTCGCACCACGACTTTAAAG‑AATTGAACTa                                                    <  1:2890702/93‑1 (MQ=255)

                                       tgTAGACGCGCTAGCTTCATGTGTTAGTGTCC‑TGACGGACGTGGGGGTTCAAG‑TC‑C‑CCC‑CCCCTCGCACCACGACTTTAAAG‑AATTGAACTaa                                                   <  1:510797/92‑1 (MQ=18)

                                       ggTAGACGCGCTAGCTTCAGGTGTTAGTGTCC‑TTACGGACGTGGGGGTTCAAG‑TC‑C‑CCC‑CCCCTCGCACCACGACTTTAAAG‑AATTGAACTaa                                                   <  1:2788687/93‑1 (MQ=255)

                                       ggTAGACGCGCTAGCTTCAGGTGTTAGTGTCC‑TTACGGACGTGGGGGTTCAAG‑TC‑C‑CCC‑CCCCTCGCACCACGACTTTAAAG‑AATTGAACTaa                                                   <  2:3940360/93‑1 (MQ=255)

                                         tAGACGCGCTAGTTTCAGGTGTTAGTGTCC‑TTATGGAAGTGGGGGTTCAAG‑TC‑C‑CCC‑CCCCTCGCACCACGACTTTAAAG‑AATTGAACTaaaa                                                 <  1:709464/93‑1 (MQ=18)

                                         tAGACGCGCTAGCTTCAGGTGTTAGTGTCC‑TTACGGACGTGGGGGTTCAAG‑TCCC‑CCC‑CCCCTCGCACCACGACTTTAAAG‑AATTGAACTaaa                                                  <  1:2364274/93‑1 (MQ=18)

                                            aCGCGCTTGGCTCCGGTGTTTGTGGCCTTTCCGGCCGTGGGGTTCCAGGCC‑‑C‑CCC‑CCCCTCGCACCACGACTTTAAAG‑AATTGAACTAAAAAt                                               <  1:1616677/93‑1 (MQ=255)

                                                gcTAGCTTCAGGTGTTAGTGTCC‑TTACGGACGTGGGGGTTCAAG‑TC‑C‑CCC‑CCCCTCGCACCACGACTTTAAAG‑AATTGAACTAAAAATTCaaa                                          >  2:711837/1‑93 (MQ=255)

                                                 cTAGCTTCAGGTGTTAGTGTCC‑TTACGGACGTGGGGGTTCAAGCC‑‑C‑CCC‑CCCCTCGCACCACGACTTTAAAG‑AATTGAACTAAAAATTCaaaa                                         <  2:3487762/93‑1 (MQ=255)

                                                 cTAGCTTCAGGTGTTAGTGTCC‑TTACGGACGTGGGGGTTCAAG‑TC‑C‑CCC‑CCCCTCGCACCACGACTTTAAAG‑AATTGAACTAAAAATTCaaaa                                         >  2:3771187/1‑93 (MQ=255)

                                                 cTAGCTTCAGGTGTTAGTGTCC‑TTACGGACGTGGGGGTTCAAG‑TC‑C‑CCC‑CCCCTCGCACCACGACTTTAAAG‑AATTGAACTAAAAATTCaaaa                                         <  2:647225/93‑1 (MQ=255)

                                                  tAGCTTCAGGTGTTAGTGTCC‑TTACGGACGTGGGGGTTCAAG‑TC‑C‑CCC‑CCCCTCGCACCACGCATTTAAAG‑AATTGAACTAAAAATTCaaaaa                                        >  1:1938878/1‑93 (MQ=18)

                                                  tAGCTTCAGGTGTTAGTGTCC‑TTACGGACGTGGGGGTTCAAG‑TC‑C‑CCC‑CCCCTCGCACCACGACTTTAAAG‑AATTGAACTAAAAATTCaaaaa                                        <  1:618812/93‑1 (MQ=255)

                                                  tAGCTTCAGGTGTTAGTGTCC‑TTACGGACGTGGGGGTTCAAG‑TC‑C‑CCC‑CCCCTCGCACCACGACTTTAAAG‑AATTGAACTAAAAATTCaaaaa                                        <  2:3448405/93‑1 (MQ=255)

                                                  tAGCTTCAGGTGTTAGTGTCC‑TTACGGACGTGGGGGTTCAAG‑TC‑C‑CCC‑CCCCTCGCACCACGACTTTAAAG‑AATTGAACTAAAAATTCaaaaa                                        <  2:3703904/93‑1 (MQ=255)

                                                  tAGCTTCAGGTGTTAGTGTCC‑TTACGGACGTGGGGGTTCAAG‑TC‑C‑CCC‑CCCCTCGCACCACGACTTTAAAG‑AATTGAACTAAAAATTCaaaaa                                        <  2:2141392/93‑1 (MQ=255)

                                                  tAGCTTCAGGTGTTAGTGTCC‑TTACGGACGTGGGGGGTCAAGAC‑‑C‑CCC‑CCCCTCGCCCCACGACCTTAAAA‑AAATGAAAGAAAAAATaaaaaa                                        >  2:1190695/1‑93 (MQ=9)

                                                    gCTTCAGGTGTTAGTGTCC‑TTACGGACGTGGGGGTTCAAG‑TC‑C‑CCC‑CCCCTCGCACCACGACTTTAAAG‑AATTGAACTAAAAATTCAAAAAGc                                      <  2:2623251/93‑1 (MQ=255)

                                                    gCTTCAGGTGTTAGTGTCC‑TTACGGACGTGGGGGTTCAAG‑TC‑C‑CCC‑CCCCTCGCACCACGACTTTAAAG‑AATTGAACTAAAAATTCAAAAAGc                                      <  1:1749733/93‑1 (MQ=255)

                                                     cTTCAGGTGTTAGTGTCC‑TTACGGACGTGGGGGTTCAAG‑TC‑C‑CCC‑CCCCTCGCACCACGACTTTAAAG‑AATTGAACTAAAAATTCAAAAAGCa                                     <  1:2740465/93‑1 (MQ=255)

                                                     cTTCAGGTGTTAGTGTCC‑TTACGGACGTGGGGGTTCAAG‑TC‑C‑CCC‑CCCCTCGCACCACGACTTTAAAG‑AATTGAACTAAAAATTCAAAAAGCa                                     <  1:1006900/93‑1 (MQ=255)

                                                      ttCAGGTGTTAGTGTAC‑TTACGGACGTGGGGGTTCAAGTT‑‑C‑CCC‑CCCCTCGCACCACGACTTTAAAG‑AATTGAACTAAAAATTCAAAAAGCAg                                    >  2:1579911/1‑93 (MQ=255)

                                                       tCAGGTGTTAGTGTCC‑TTACGGACGTGGGGGTTCAAG‑TC‑C‑CCC‑CCCCTCGCACCACGACTTTAAAG‑AATTGAACTAAAAATTCAAAAAGCAGt                                   <  1:1992194/93‑1 (MQ=255)

                                                       tCAGGTGTTAGTGTCC‑TTACGGACGTGGGGGTTCAAG‑TC‑C‑CCC‑CCCCTCGCACCACGACTTTAAAG‑AATTGAACTAAAAATTCAAAAAGCAGt                                   >  2:2874284/1‑93 (MQ=255)

                                                       tCAGGTGTTAGTGTCC‑TTACGGACGTGGGGGTTCAAG‑TC‑C‑CCC‑CCCCTCCCACCACGACCTTAAAGAAATTAAACAAAAAATTaaaaaacagt                                    >  2:3355572/1‑89 (MQ=17)

                                                        cAGGTGTTAGTGTCC‑TTACGGACGTGGGGGTTCAAG‑TC‑C‑CCC‑CCCCTCGCACCACGACTTTAAAG‑AATTGAACTAAAAATTCAAAAAGCAGTa                                  <  2:4176875/93‑1 (MQ=255)

                                                        cAGGTGTTAGTGTCC‑TTACGGACGTGGGGGTTCAAG‑TC‑C‑CCC‑CCCCTCGCACCACGACTTTAAAG‑AATTGAACTAAAAATTCAAAAAGCAGTa                                  <  1:2949359/93‑1 (MQ=255)

                                                        cAGGTGTTAGTGTCC‑TTACGGACGTGGGGGTTCAAG‑TC‑C‑CCC‑CCCCTCGCACCACGACTTTAAAG‑AATTGAACTAAAAATTCAAAAAGCAGTa                                  <  1:4633058/93‑1 (MQ=255)

                                                         aGGTGTTAGTGTCC‑TTACTGACGTGGGGGTTCAAG‑TC‑C‑CCC‑CCCCTCGCACCACGACTTTAAAG‑AATTGAACTAAAAATTCAAAAAGCAGTAt                                 <  2:243608/93‑1 (MQ=255)

                                                          ggTGTTAGTTTCC‑TTACGGACGTGGGGGTTCAAG‑TC‑C‑CCC‑CCCCTCGCACCACGACTTTAAAG‑AATTGAACTAAAAATTCAAAAAGCAGTAtt                                <  2:3289878/93‑1 (MQ=255)

                                                          ggTGTTAGTGTCC‑TTACGGACGTGGGGGTTCAAG‑TC‑C‑CCC‑CCCCTCGCACCACGACTTTAAAG‑AATTGAACTAAAAATTCAAAAAGCAGTAtt                                >  1:4335298/1‑93 (MQ=255)

                                                          ggTGTTAGTGTCC‑TTACGGACGTGGGGGTTCAAG‑TC‑C‑CCC‑CCCCTCGCACCACGACTTTAAAG‑AATTGAACTAAAAATTCAAAAAGCAGTAtt                                <  1:837898/93‑1 (MQ=255)

                                                           gtgtTAGTGTCC‑TTACGGACGTGGGGGTTCAAG‑TC‑C‑CCC‑CCCCTCGCACCACGACTTTAAAG‑AATTGAACTAAAAATTCAAAAAGCAGTAttt                               <  2:250238/93‑1 (MQ=255)

                                                           gtgtTAGTGTCC‑TTACGGACGTGGGGGTTCAAG‑TC‑C‑CCC‑CCCCCCGCACCCCGACTTTAAAA‑AATTGAACTAAAAATTCAAAAAACAGTAttt                               >  2:4099013/1‑93 (MQ=21)

                                                            tgtTAGTGTCC‑TTACGGACGTGGGGGTTCAAG‑TC‑C‑CCC‑CCCCTCGCACCACGACTTTAAAG‑AATTGAACTAAAAATTCAAAAAGCAGTATTTc                              <  1:3188882/93‑1 (MQ=255)

                                                            tgtTAGTGTCC‑TTACGGACGTGGGGGTTCAAG‑TC‑C‑CCC‑CCCCTCGCACCACGACTTTAAAG‑AATTGAACTAAAAATTCAAAAAGCAGTATTTc                              <  2:2028932/93‑1 (MQ=255)

                                                            tgtTAGGGTCC‑TTACGGACGTGGGGGTTCAAGCC‑‑C‑CCC‑CCCCTCGCACCACGACTTTAAAG‑AATTGAACTAAAAATTCAAAAAGCAGTATTTc                              <  2:4451718/93‑1 (MQ=37)

                                                             gtTAGTGTCC‑TTACGGACGTGGGGGTTCAAG‑TC‑C‑CCC‑CCCCTCGCACCACGACTTTAAAG‑AATTGAACTAAAAATTCAAAAAGCAGTATTTCg                             <  1:3493806/93‑1 (MQ=255)

                                                              ttAGTGTCC‑TTACGGACGTGGGGGTTCAAG‑TC‑C‑CCC‑CCCCTCGCACCACGACTTTAAAG‑AATTGAACTAAAAATTCAAAAAGCAGTATTTCgg                            >  1:2101058/1‑93 (MQ=255)

                                                                    tCC‑TTACGGACGTGGGGGTTCAAG‑TC‑C‑CCC‑CCCCTCGCACCACGACTTTAAAG‑AATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTa                      <  2:2226140/93‑1 (MQ=255)

                                                                    tCC‑TTACGGACGTGGGGGTTCAAG‑TC‑C‑CCC‑CCCCTCGCACCACGACTTTAAAG‑AATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTa                      >  2:1265822/1‑93 (MQ=255)

                                                                      c‑TTACGGACGTGGGGGTTCAAG‑TC‑C‑CCC‑CCCCTCGCACCACGACTTTAAAG‑AATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAgc                    <  2:4241163/93‑1 (MQ=255)

                                                                      c‑TTACGGACGTGGGGGTTCAAG‑TC‑C‑CCC‑CCCCTCGCACCACGACTTTAAAG‑AATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAgc                    <  2:496947/93‑1 (MQ=255)

                                                                         tACGGACGTGGGGGTTCAAG‑TC‑C‑CCC‑CCCCTCGCACCACGACTTTAAAG‑AATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAgcgc                  >  1:4542933/1‑93 (MQ=255)

                                                                           cGGACGTGGGGGTTCAAG‑TC‑C‑CCC‑CCCCTCGCACCACGACTTTAAAG‑AATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAGCGCAg                <  2:1521722/93‑1 (MQ=255)

                                                                            ggACGTAGGGGTAAAAG‑T‑‑C‑CCCTCCCCTCGCACCACGACTTTAAAG‑AATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAGCGCAGc               <  1:2167368/93‑1 (MQ=255)

                                                                            ggACGGGGGGGTTCAAG‑TC‑C‑CCC‑CCCCTCGCACCACGACTTTAAAG‑AATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAGCGCAGc               <  1:349720/93‑1 (MQ=255)

                                                                             gACGTGGGGGTTCAAGCC‑‑C‑CCC‑CCCCTCGCACCACGACTTTAAAG‑AATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAGCGCAGCt              <  1:2471170/93‑1 (MQ=255)

                                                                             gACGTGGGGGTTCAAG‑TC‑C‑CCC‑CCCCTCGCACCACGACTTTAAAG‑AATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAGCGCAGCt              >  2:1999677/1‑93 (MQ=255)

                                                                             gACGTGGGGGTTCAAG‑TC‑C‑CCC‑CCCCTCGCACCACGACTTTAAAG‑AATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAGCGCAGCt              >  2:2313599/1‑93 (MQ=255)

                                                                             gACGTGGGGAGTCAAG‑C‑‑CACCC‑CCCCTCGCACCACGACTATAAAG‑AATTGAACTAAAAATTCAAAAAGCACTATTTCGGCGAGTAGCGCAGCt              <  2:1974481/93‑1 (MQ=255)

                                                                              aCGTGGGGGTTCAAG‑TC‑C‑CCC‑CCCCTCGCACCACGACTTTAAAG‑AATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAGCGCAGCtt             >  1:4663774/1‑93 (MQ=255)

                                                                                gTGGGGGTTCAAG‑TC‑C‑CCC‑CCCCTCGCACCACGACTTTAAAG‑AATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAGCGCAGCTTgg           <  1:3042561/93‑1 (MQ=255)

                                                                                    gggTTCAAG‑TC‑C‑CCC‑CCCCTCGCACCACGACTTTAAAG‑AATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAGCGCAGCTTGGTAgc       <  2:270553/93‑1 (MQ=255)

                                                                                      gTTCAAG‑TC‑C‑CCC‑CCCCTCGCACCACGACTTTAAAG‑AATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAGCGCAGCTTGGTAgcgc     <  2:1736983/93‑1 (MQ=255)

                                                                                       ttCAAG‑T‑‑C‑CCC‑CCCCTCGCACCACGACTTTAAAG‑AATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAGCGCAGCTTGGTAGCGCaa   >  2:3167783/1‑93 (MQ=255)

                                                                                       ttCAAG‑T‑‑C‑CCC‑CCCCTCGCACCACGACTTTAAAG‑AATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAGCGCAGCTTGGGAGCGCaa   >  1:4265354/1‑93 (MQ=255)

                                                                                         cAAG‑TC‑C‑CCC‑CCCCTCGCACCACGACTTTAAAG‑AATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAGCGCAGCTTGGTAGCGCAAc  <  2:1460657/93‑1 (MQ=255)

                                                                                         cAAG‑TC‑C‑CCC‑CCCCTCGCACCACGACTTTAAAG‑AATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAGCGCAGCTTGGTAGCGCAAc  <  1:2987813/93‑1 (MQ=255)

                                                                                         cAAG‑TC‑C‑CCC‑CCCCTCGCACCACGACTTTAAAG‑AATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAGCGCAGCTTGGTAGCGCAAc  <  1:2592241/93‑1 (MQ=255)

CATTATTAGAAGTTGTGACAATGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCC‑TTACGGACGTGGGGGTTCAAG‑T‑‑C‑CCC‑CCCCTCGCACCACGACTTTAAAG‑AATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAGCGCAGCTTGGTAGCGCAAC  >  NC_000913/3980197‑3980376

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 16 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`