BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,435,684	+A	coding (400/501 nt)	ytfI →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,435,681	1	.	A	100.0%	656.0 / NA	177	Q133K (CAA→AAA)	ytfI	hypothetical protein
Reads supporting (aligned to +/- strand): ref base . (0/0); new base A (95/82); total (95/82)

Only 100 of 195 total aligned reads displayed.

GAGGTAGTGGATTTGAATACGAAGAAATACCCATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAAC‑AAACAGACGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAGGTTATATTCACCAGGCGAT  >  NC_000913/4435589‑4435768

     aGTGGATTTGAATACGAAGAAATACCCATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGaac‑aaaac                                                                                    <  2:3880182/93‑3 (MQ=255)

     aGTGGATTTGAATACGAAGAAATACCCATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGaac‑aaaac                                                                                    >  2:3518608/1‑91 (MQ=255)

      gTGGATTTGAATACGAAGAAATACCCATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGaac‑aaaaca                                                                                   <  1:335601/93‑4 (MQ=255)

      gTGGATTTGAATACGAAGAAATACCCATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGaac‑aaaaca                                                                                   <  1:1681015/93‑4 (MQ=255)

      gTGGATTTGAATACGAAGAAATACCCATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGaac‑aaaaca                                                                                   >  1:855054/1‑90 (MQ=255)

            ttGAATACGAAGAAATACCCATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAgatgat                                                                              <  1:382109/93‑1 (MQ=255)

              gAATACGAAGAAATACCCATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGATGATTg                                                                            <  1:3967912/93‑1 (MQ=255)

                aTACGAAGAAATACCCATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGATGATTGTa                                                                          <  2:376413/93‑1 (MQ=255)

                 tACGAAGAAATACCCATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGATGATTGTAt                                                                         >  2:2353866/1‑93 (MQ=255)

                 aaCGAAGAAATACCCATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGATGATTGTAt                                                                         <  2:1733602/92‑1 (MQ=255)

                   cGAAGAAATACCCATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGATGATTGTATGc                                                                       >  1:750753/1‑93 (MQ=255)

                    gaagaaATACCCATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGATGATTGTATGct                                                                      >  1:3929686/1‑92 (MQ=255)

                        aaaTACCCATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGATGATTGTATGCCTggg                                                                  >  1:283554/1‑93 (MQ=255)

                          aTACCCATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGATGATTGTATGCCTGGGat                                                                <  1:1731885/93‑1 (MQ=255)

                           tACCCATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGATGATTGTATGCCTGGGtta                                                               <  2:2935695/93‑1 (MQ=255)

                           tACCCATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGATGATTGTATGCCTGGGata                                                               <  1:2032626/93‑1 (MQ=255)

                             cccATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATAtt                                                             <  2:1493561/93‑1 (MQ=255)

                             cccATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATAtt                                                             >  1:3094186/1‑93 (MQ=255)

                              ccATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATATTc                                                            >  1:2889914/1‑93 (MQ=255)

                              ccATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATATTc                                                            >  2:4289001/1‑93 (MQ=255)

                               cATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATAttat                                                           >  1:3697354/1‑91 (MQ=255)

                                 tAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATATTCTAc                                                         >  1:2681605/1‑93 (MQ=255)

                                 tAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATATTCTAc                                                         >  2:751985/1‑93 (MQ=255)

                                 tAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATATTCTAc                                                         >  2:247388/1‑93 (MQ=255)

                                  aaCAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATATTCTACa                                                        <  2:4216899/93‑1 (MQ=255)

                                       tAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATATTCTACAGaaaa                                                   >  1:1027078/1‑93 (MQ=255)

                                       tAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATATTCTACAGaaaa                                                   >  2:3202509/1‑93 (MQ=255)

                                       tAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATATTCTACAGaaaa                                                   >  2:1802319/1‑93 (MQ=255)

                                         aaTGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATATTCTACAGaaaaaa                                                 <  1:2545054/93‑1 (MQ=255)

                                         aaTGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATATTCTACAGaaaaaa                                                 <  2:4615635/93‑1 (MQ=255)

                                         aaTGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATATTCTACAGaaaaaa                                                 >  1:142238/1‑93 (MQ=255)

                                          aTGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATATTCTACAGAAAAAAc                                                >  1:439065/1‑93 (MQ=255)

                                          aTGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATATTCTACAGAAAAAAc                                                <  1:2644880/93‑1 (MQ=255)

                                           tGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACa                                               >  2:2629792/1‑93 (MQ=255)

                                            gAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAg                                              >  1:3430049/1‑93 (MQ=255)

                                             aaTATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGt                                             <  1:1040275/93‑1 (MQ=255)

                                              atatAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTc                                            >  2:2678517/1‑93 (MQ=255)

                                              atatAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTc                                            <  2:578112/93‑1 (MQ=255)

                                              atatAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTc                                            <  2:1413008/93‑1 (MQ=255)

                                               tataACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCt                                           >  1:246486/1‑93 (MQ=255)

                                               tataACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCt                                           >  1:1883720/1‑93 (MQ=255)

                                               tataACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCt                                           <  1:1889585/93‑1 (MQ=255)

                                               tataACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCt                                           <  1:1504023/93‑1 (MQ=255)

                                               tataACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCt                                           <  1:2959085/93‑1 (MQ=255)

                                                 taACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGa                                         >  2:683864/1‑93 (MQ=255)

                                                  aaCTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGAc                                        >  1:2010672/1‑93 (MQ=255)

                                                   aCTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACa                                       <  2:284264/93‑1 (MQ=255)

                                                     tcattcatGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATa                                     >  1:2111329/1‑93 (MQ=255)

                                                      cattcatGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATAc                                    <  2:654225/93‑1 (MQ=255)

                                                      cattcatGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATAc                                    <  1:3675953/93‑1 (MQ=255)

                                                       attcatGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATAcc                                   <  2:4125525/93‑1 (MQ=255)

                                                       attcatGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATAcc                                   <  2:79315/93‑1 (MQ=255)

                                                        ttcatGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCg                                  >  1:521869/1‑93 (MQ=255)

                                                         tcatGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGa                                 >  2:1911394/1‑93 (MQ=255)

                                                          catGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAATCTAACATACCGaa                                >  2:3055162/1‑93 (MQ=255)

                                                           atGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGaaa                               >  2:2788810/1‑93 (MQ=255)

                                                           atGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGaaa                               >  2:3492355/1‑93 (MQ=255)

                                                           atGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGaaa                               >  1:4062030/1‑93 (MQ=255)

                                                            tGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGaaaa                              <  1:3509949/93‑1 (MQ=255)

                                                            tGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGaaaa                              <  2:4413295/93‑1 (MQ=255)

                                                             ggATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAg                             <  2:110321/93‑1 (MQ=255)

                                                             ggATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAg                             <  1:1218953/93‑1 (MQ=255)

                                                              gATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAgg                            >  1:4642839/1‑93 (MQ=255)

                                                               aTTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGa                           >  1:3203886/1‑93 (MQ=255)

                                                               aTTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGa                           >  2:1301530/1‑93 (MQ=255)

                                                                tttCAAAAACCGTGAATTTCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGAt                          >  1:1312078/1‑93 (MQ=255)

                                                                tttCAAAAACCGTGAATTTCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGAt                          <  2:2247964/93‑1 (MQ=255)

                                                                 ttCAAAAACCGTGAATTTCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGAtt                         <  2:1815564/93‑1 (MQ=255)

                                                                 ttCAAAAACCGTGAATTTCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGAtt                         <  2:2064156/93‑1 (MQ=255)

                                                                 ttCAAAAACCGTGAATTTCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGAtt                         <  1:112656/93‑1 (MQ=255)

                                                                  tCAAAAACCGTGAATTTCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTa                        <  1:4257709/93‑1 (MQ=255)

                                                                   cAAAAACCGTGAATTTCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTaa                       <  2:510773/93‑1 (MQ=255)

                                                                   cAAAAACCGTGAATTTCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTaa                       >  1:1576275/1‑93 (MQ=255)

                                                                    aaaaaCCGTGAATTTCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTaaa                      >  2:885846/1‑93 (MQ=255)

                                                                     aaaaCCGTGAATTTCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAg                     <  2:296939/93‑1 (MQ=255)

                                                                     aaaaCCGTGAATTTCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAg                     >  2:1131394/1‑93 (MQ=255)

                                                                     aaaaCCGTGAATTTCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAg                     <  1:3875278/93‑1 (MQ=255)

                                                                     aaaaCCGTGAATTTCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAg                     >  1:2954423/1‑93 (MQ=255)

                                                                      aaaCCGTGAATTTCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAgg                    <  1:1505502/93‑1 (MQ=255)

                                                                        aCCGTGAATTTCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAGGtt                  >  2:3269692/1‑93 (MQ=255)

                                                                         ccGTGAATTTCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAGGTta                 <  1:3850917/93‑1 (MQ=255)

                                                                         ccGTGAATTTCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAGGTta                 <  1:2434290/93‑1 (MQ=255)

                                                                          cGTGAATTTCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAACAGGATTAAAGGTtaa                >  2:186903/1‑92 (MQ=255)

                                                                          cGTGAATTTCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAGGTtat                <  1:4673338/93‑1 (MQ=255)

                                                                          cGTGAATTTCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAGGTtat                <  2:4019350/93‑1 (MQ=255)

                                                                          cGTGAATTTCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAGGTtat                <  1:214070/93‑1 (MQ=255)

                                                                           gTGAATTTCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAGGTtata               <  1:2578754/93‑1 (MQ=255)

                                                                           gTGAATTTCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAGGTtata               <  1:3894748/93‑1 (MQ=255)

                                                                            tGAATTTCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAGGTTatat              >  1:3230337/1‑93 (MQ=255)

                                                                              aaTTTCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAGGTTATATTc            <  1:2660420/93‑1 (MQ=255)

                                                                               aTTTCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAGGTTATATTCa           <  2:3341320/93‑1 (MQ=255)

                                                                                tttCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAGGTTATATTCAc          >  2:4497207/1‑93 (MQ=255)

                                                                                tttCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAGGTTATATTCAc          >  2:2106225/1‑93 (MQ=255)

                                                                                 ttCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAGGTTATATTCAcc         <  1:3708480/93‑1 (MQ=255)

                                                                                    cGTTAGAACAAAACAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAGGTTATATTCACCAgg      <  1:1343670/93‑1 (MQ=255)

                                                                                    cGTTAGAACAAAACAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAGGTTATATTCACCAgg      <  2:4060682/93‑1 (MQ=255)

                                                                                     gTTAGAACAAAACAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAGGTTATATTCACCAGGc     >  2:4373424/1‑93 (MQ=255)

                                                                                      ttAGAACAAAACAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAGGTTATATTCACCAGGCg    <  2:2288043/93‑1 (MQ=255)

                                                                                      ttAGAACAAAACAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAGGTTATATTCACCAGGCg    >  1:3021248/1‑93 (MQ=255)

                                                                                        aGAACAAAACAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAGGTTATATTCACCAGGCgct  >  2:2817197/1‑91 (MQ=255)

GAGGTAGTGGATTTGAATACGAAGAAATACCCATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAAC‑AAACAGACGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAGGTTATATTCACCAGGCGAT  >  NC_000913/4435589‑4435768

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 26 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`