BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,603,784	(G)_8→9	noncoding (72/87 nt)	leuP ←	tRNA‑Leu

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,603,776	1	.	G	84.9%	307.4 / 59.5	127	noncoding (80/87 nt)	leuP	tRNA‑Leu
Reads supporting (aligned to +/- strand): ref base . (17/2); new base G (77/30); total (95/32)
Fisher's exact test for biased strand distribution p-value = 1.53e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.85e-01

Only 100 of 162 total aligned reads displayed.

AAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑‑GG‑GGGGGGACT‑TGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAG  >  NC_000913/4603684‑4603865

aaGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGa                                                                                               <  1:4237917/93‑1 (MQ=255)

aaGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGa                                                                                               <  2:4620762/93‑1 (MQ=255)

aaGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGa                                                                                               <  1:1530329/93‑1 (MQ=255)

 aGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑‑g                                                                                            <  2:3317596/93‑1 (MQ=255)

 aGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑‑g                                                                                            >  1:2050751/1‑93 (MQ=255)

  ggACACTAACCCCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑‑gg                                                                                           <  1:37362/93‑1 (MQ=255)

  ggACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑‑gg                                                                                           <  2:4009804/93‑1 (MQ=255)

    acacTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑‑gg‑gg                                                                                        <  1:1123706/93‑1 (MQ=255)

    acacTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑‑gg‑gg                                                                                        >  2:2540244/1‑93 (MQ=255)

    acacTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑‑gg‑gg                                                                                        <  2:3963921/93‑1 (MQ=255)

    acacTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑‑gg‑gg                                                                                        >  2:3427790/1‑93 (MQ=255)

     cacTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑‑gg‑ggg                                                                                       <  2:1055137/93‑1 (MQ=255)

      acTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑‑gg‑gggg                                                                                      >  2:2608382/1‑93 (MQ=255)

      acTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑‑gg‑gggg                                                                                      >  2:1476269/1‑93 (MQ=255)

       cTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑‑gg‑gggtg                                                                                     >  1:2640472/1‑91 (MQ=255)

       cTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑‑gg‑ggggg                                                                                     <  2:26743/93‑1 (MQ=255)

       cTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑‑gg‑ggggg                                                                                     >  1:884109/1‑93 (MQ=255)

        tAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑‑gg‑gggggg                                                                                    >  1:4457765/1‑93 (MQ=255)

         aaCACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑‑gg‑ggggggg                                                                                   >  1:806850/1‑92 (MQ=255)

         aaCACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑‑gg‑ggggggg                                                                                   <  2:1215996/93‑2 (MQ=255)

            acCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑‑gg‑gggggggggt                                                                                >  1:1095713/1‑89 (MQ=255)

                  aGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAG‑GG‑GGGGGGGCT‑TGAAcc                                                                          <  1:2841457/93‑1 (MQ=37)

                     tAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAG‑GG‑GGGGGGGCT‑TGAAccccc                                                                       <  2:625287/93‑1 (MQ=38)

                     tAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAG‑GG‑GGGGGGGCT‑TCAAccccc                                                                       >  2:648009/1‑93 (MQ=38)

                      aGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAG‑GG‑GGGGGGGCT‑TGAACCCCCa                                                                      <  2:695475/93‑1 (MQ=38)

                         gcgTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAG‑GG‑GGGGGGGCT‑TGAACCCCCACgc                                                                   >  1:1036462/1‑92 (MQ=39)

                         gcgTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAG‑GG‑GGGGGGGCT‑TGAACCCCCACGt                                                                   >  1:3314533/1‑93 (MQ=255)

                         gcgTCTACCAATCCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCCCGTTTGGTGGGAG‑GG‑GGGGGGGCT‑TGAACCCCCACGt                                                                   <  2:4441447/93‑1 (MQ=38)

                          cgTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAG‑GG‑GGGGGGGCT‑TGAACCCCCACGTc                                                                  <  1:3559278/93‑1 (MQ=39)

                           gTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAG‑GG‑GGGGGGGCT‑TGAACCCCCACGTcc                                                                 >  1:97255/1‑93 (MQ=255)

                           gTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAG‑GG‑GGGGGGGCT‑TGAACCCCCACGTcc                                                                 >  2:2366750/1‑93 (MQ=255)

                           gTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAG‑GG‑GGGGGGGCT‑TGAACCCCCACGTcc                                                                 >  1:983041/1‑93 (MQ=255)

                           gTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAG‑GG‑GGGGGGGCT‑TGAACCCCCACGTcc                                                                 >  2:3089501/1‑93 (MQ=255)

                           gTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAG‑GG‑GGGGGGGCT‑TGAACCCCCACGTcc                                                                 <  1:1778288/93‑1 (MQ=39)

                            tCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGCTGCGA‑‑GGTGCGGGGGGT‑TGAACCCCCACGTCCg                                                                >  1:363312/1‑93 (MQ=38)

                             cTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAG‑GG‑GGGGGGGCT‑TGAAACCCCACGTCCga                                                               >  2:2902517/1‑92 (MQ=38)

                               aCCAATTCCGCCCCCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAG‑GG‑GGGGGGGCT‑TGAACCCCCACGTCCGTaa                                                             <  1:1882379/93‑1 (MQ=38)

                                ccAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑‑GG‑GGGGGGGCT‑TGAACCCCCACGTCCGTAAGa                                                           >  1:880243/1‑93 (MQ=255)

                                 cAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAG‑GG‑GGGGGGGCT‑TGAACCCCCACGTCCGTAAGa                                                           >  2:2162199/1‑93 (MQ=255)

                                 cAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑‑GG‑GGGGGGGCT‑TGAACCCCCACGTCCGTAAGaa                                                          >  2:1644148/1‑93 (MQ=255)

                                  aaTTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAG‑GG‑GGGGGGGCT‑TGAACCCCCACGTCCGTAAGaa                                                          >  1:234276/1‑93 (MQ=255)

                                  aaTTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑‑GG‑GGGGGGGCT‑TGAACCCCCACGTCCGTAAGaaa                                                         >  2:2774573/1‑92 (MQ=255)

                                  aaTTCCGCCACCGTCGCACATTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAG‑GG‑GGGGGGGCT‑TGAACCCCCACGTCCGTAAGaa                                                          <  2:4600366/93‑1 (MQ=37)

                                          cACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAG‑GG‑GGGGGGGCT‑TGAACCCCCACGTCCGTAAGAACACTAacc                                                  >  1:4166724/1‑92 (MQ=37)

                                          cACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAG‑GG‑GGGGGGGCT‑TGAACCCCCACGTCCGTAAGAACACTAaca                                                  >  2:1651138/1‑93 (MQ=255)

                                          cACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑‑GG‑GGGGGGGCT‑TGAACCCCCACGTCCGTAAGAACACTAacac                                                 >  2:1891705/1‑93 (MQ=255)

                                           aCCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑‑GG‑GGGGGGGCT‑TGAACCCCCACGTCCGTAAGAACACTAACAcc                                                >  1:318407/1‑93 (MQ=255)

                                             cTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAG‑GG‑GGGGGGGCT‑TGAACCCCCACGTCCGTAAGAACACTAACACCt                                               <  2:1299143/93‑1 (MQ=255)

                                                cacacaGTCATTCTGCTTTTTTTGATTGCGCCTCGTTTGGTGCGAG‑GG‑GGGGGGGCT‑TGAACCCCCACGTCCGTAAGAACACTAACACCTGaa                                            <  1:2313467/91‑1 (MQ=18)

                                                cGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAG‑GG‑GGGGGGGCT‑TGAACCCCCACGTCCGTAAGAACACTAACACCTGaa                                            <  2:4219172/93‑1 (MQ=255)

                                                cGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAG‑GG‑GGGGGGGCT‑TGAACCCCCACGTCCGTAAGAACACTAACACCTGaa                                            <  2:4683192/93‑1 (MQ=255)

                                                 gCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAG‑GG‑GGGGGGGCT‑TGAACCCCCACGTCCGTAAGAACACTAACACCTGAAg                                           >  2:4403130/1‑93 (MQ=255)

                                                   acaGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAC‑GG‑GGGGGGGGTGTGAACCCCCACCTCCGTAAGAACACTAACACCTaaatc                                          >  1:2516554/1‑91 (MQ=255)

                                                    caGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGGGG‑GGGGGGGCT‑TGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCt                                         <  1:1209157/93‑1 (MQ=25)

                                                    caGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAG‑GG‑GGGGGGGGT‑TGAACCCCCACGTCCGTAAGAAAACTAACACCTGAAGCTa                                        >  2:651037/1‑93 (MQ=21)

                                                    caGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAG‑GG‑GGGGGGGCT‑TGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTa                                        >  1:2884865/1‑93 (MQ=255)

                                                    caGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAG‑GG‑GGGGGGGAT‑TGAACCCCCACGGCCGTAAGAACACTAACACCTGAAGATa                                        >  2:1326549/1‑93 (MQ=21)

                                                     aGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAG‑GG‑GGGGGGGCT‑TGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAg                                       <  2:719635/93‑1 (MQ=255)

                                                      gTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAG‑GG‑GGGGGGGCT‑TGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAgg                                      >  1:512311/1‑92 (MQ=21)

                                                       tCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAG‑GG‑GGGGGGGCT‑TGAACCCCCACGTCCGGAAGAACACTAACACCTGAAGCTAgcg                                     >  1:4676766/1‑93 (MQ=21)

                                                        cATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAG‑GG‑GGGGGGGCT‑TGAACCCCCACGTCCGTAAGAAAACTAACACCTGAAGCTaaccg                                    >  1:3837748/1‑89 (MQ=21)

                                                         aTCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAG‑GG‑GGGGGGGCT‑TGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGcgcg                                   >  2:3204636/1‑93 (MQ=255)

                                                         aTCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAG‑GG‑GGGGGGGCT‑TGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGcgcg                                   >  1:3352644/1‑93 (MQ=255)

                                                         aTCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAG‑GG‑GGGGGGGCT‑TGAACCCCCACGTCCGTAAGAACACTAACACCTGAAACTAGcgcg                                   >  2:1537465/1‑93 (MQ=21)

                                                          tCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAG‑GG‑GGGGGGGCT‑TGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGt                                  >  1:4180217/1‑93 (MQ=255)

                                                           cTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAG‑GG‑GGGGGGGCT‑TTAAACCCCACGGCCGCTAAAAAACTTAAAACTGGAGCTAGCGCGTc                                 >  1:1128775/1‑93 (MQ=16)

                                                           cTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAG‑GG‑GGGGGGGCT‑TGAGCCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTc                                 >  2:826572/1‑93 (MQ=18)

                                                           cTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAG‑GG‑GGGGGGGCT‑TGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTc                                 >  1:557116/1‑93 (MQ=255)

                                                            ttACTTTTTTTGATATCGCCTCGTTTGGTGCGAG‑GG‑GGGGGGGCT‑TGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCt                                >  2:2151808/1‑93 (MQ=255)

                                                            ttACTTTTTTTGATATCGCCTCGTTTGGTGCGAG‑GG‑GGGGGGGCT‑TGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCt                                >  1:1434715/1‑93 (MQ=255)

                                                             ttcTTTTTTTGTTATGACGTCGTTCGGTTGGAG‑GG‑GGGGGGGCG‑TGAACCCCCGCGTCCGTAAGAACACTAGCGCCTGAAGCTAGCGCGTCTa                               <  2:708338/91‑1 (MQ=9)

                                                              aCTTTTTTTGATATCGCCTCGTTTGGTGCGAG‑GG‑GGGGGGGCT‑TGAACCCCCCCGTCCCTAAGAACACTAACACCTGAAGCTAGCGCCTCTAc                              >  1:1564000/1‑93 (MQ=18)

                                                              aCTTTTTTTGATATCGCCTCGTTTGGTGCGAG‑GG‑GGGGGGGCT‑TGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTAc                              >  2:4523006/1‑93 (MQ=255)

                                                                tttttttGATATCGCCTCGTTTGGTGCGAG‑GG‑GGGGGGGCT‑TGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCa                            >  2:970469/1‑93 (MQ=255)

                                                                tttttttGATATCGCCTCGTTTGGTGCGAG‑GG‑GGGGGGGCT‑TGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCa                            >  1:4049608/1‑93 (MQ=255)

                                                                  tttttGATATCGCCTCGTTTGGTGCGAG‑GG‑GGGGGGGCT‑TGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAAt                          <  1:2381076/93‑1 (MQ=255)

                                                                      tGATATCGCCTCGTTTGGTGCAA‑‑GG‑GGGGGGACT‑TGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGc                     >  1:794469/1‑93 (MQ=255)

                                                                      tGAAATCGCCTCGTTTGGGGCGAG‑GG‑GGGGGGGCT‑TGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCg                      <  2:3674605/93‑1 (MQ=17)

                                                                       gATATCGCCTCGTTTGGTGCGAG‑GG‑GGGGGGGCT‑TGAACCCCCACGTCCGTAAGCACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGc                     >  1:1157610/1‑93 (MQ=14)

                                                                       gATATCGCCTCGTTTGGTGCGAG‑GG‑GGGGGGGCT‑TGAACCCCCACGCCCGTAAGAACACTAACACCTGAAGCTAGGGCGTCTAACAATTCCGc                     >  2:4674526/1‑93 (MQ=17)

                                                                         tatCGCCTCGTTTGGTGCGAGGGG‑GGGGGGGCT‑TGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGcc                    >  1:2273749/1‑93 (MQ=14)

                                                                         tatCGCCTCGTTTGGTGCGAG‑GG‑GGGGGGGCT‑TGAACCCCCACGTCCGTAAGAACACTAACACCTAAAGCTAGCGCGTCTACCAATTCCGccc                   >  2:2464440/1‑92 (MQ=14)

                                                                           tCGCCTCGTTTGGTGCGAG‑GG‑GGGGGGGCT‑TGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCAct                 >  2:3814161/1‑92 (MQ=14)

                                                                           tCGCCTCGTTTGGTGCGAG‑GG‑GGGGGGGCT‑TGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCAcc                 >  1:24837/1‑93 (MQ=255)

                                                                            cGCCTGGTTGGGTGGGGG‑GG‑GGGGGGGCT‑TGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCt                <  2:1734414/93‑1 (MQ=11)

                                                                            cGCCTCGTTTGGTGCGAG‑GG‑GGGGGGGCT‑TGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCt                <  1:4398499/93‑1 (MQ=255)

                                                                             gCCTCGTTTGGTGCGAG‑GG‑GGGGGGGCT‑TGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCtt               >  1:1824097/1‑93 (MQ=255)

                                                                             gCCTCGTTTGGTGCGAG‑GG‑GGGGGGGCT‑TGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCtt               >  2:7011/1‑93 (MQ=255)

                                                                               cTCGTTTGGTGCGAGGGG‑GGGGGGGCT‑TGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTc              >  1:435062/1‑93 (MQ=14)

                                                                               cTCGTTTGGTGCGAG‑GG‑GGGGGGGCT‑TGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCg             <  2:1579505/93‑1 (MQ=255)

                                                                               cTCGTTTGGTGCGAG‑GG‑GGGGGGGCT‑TGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCg             >  1:826919/1‑93 (MQ=255)

                                                                               cTCGTTTGGTGCGAG‑GG‑GGGGGGGCT‑TGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGCCTACCAATTCCGCCACCTTcc             >  1:2270793/1‑92 (MQ=14)

                                                                                tCGTTTGGTGCGAG‑GG‑GGGGGGGCT‑TGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCCCCTTCGc            >  1:1229480/1‑93 (MQ=11)

                                                                                tCGTTTGGTGCGAG‑GG‑GGGGGGGCT‑TGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGc            >  2:3922813/1‑93 (MQ=255)

                                                                                tCGTTTGGTGCGAG‑GG‑GGGGGGGCT‑TGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCCCCACCTTCGc            >  2:1275274/1‑93 (MQ=11)

                                                                                  gTTTGGTGCGAG‑GG‑GGGGGGGCT‑TGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGcca          >  2:1986628/1‑92 (MQ=11)

                                                                                   tttGGTGCGAG‑GG‑GGGGGGGCT‑TGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCa         >  1:2011341/1‑93 (MQ=255)

                                                                                       gTGCGAG‑GG‑GGGGGGGCT‑TGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGc     <  1:3496064/93‑1 (MQ=255)

                                                                                       gTGCGA‑‑GG‑GGGGGGGCT‑TGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCg    <  2:1092833/93‑1 (MQ=255)

                                                                                         gCGA‑‑GG‑GGGGGGGCT‑TGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAg  >  1:3614943/1‑93 (MQ=255)

AAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑‑GG‑GGGGGGACT‑TGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAG  >  NC_000913/4603684‑4603865

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 23 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`