BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	547,835	+G	coding (255/261 nt)	b4507 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	547,831	1	.	G	98.6%	438.5 / ‑1.4	141	coding (251/261 nt)	b4507	hypothetical protein
Reads supporting (aligned to +/- strand): ref base . (0/2); new base G (76/63); total (76/65)
Fisher's exact test for biased strand distribution p-value = 2.11e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

Only 100 of 173 total aligned reads displayed.

GATGACCGGACCCATGAAAGGGGCGTGCGTGGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCA‑G‑GGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTATTACTTCTGCCAGTATGCCGATGCTGG  >  NC_000913/547739‑547916

gaTGACCGGACCCATGAAAGGGGCGTGCGTGGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCa                                                                                         >  2:581720/1‑93 (MQ=255)

gaTGACCGGACCCATGAAAGGGGCGTGCGTGGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCa                                                                                         <  2:229136/93‑1 (MQ=255)

 aTGACCGGACCCATGAAAGGGGCGTGCGTGGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCA‑g                                                                                       >  2:1309640/1‑93 (MQ=255)

 aTGACCGGACCCATGAAAGGGGCGTGCGTGGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCA‑g                                                                                       <  2:2384968/93‑1 (MQ=255)

   gACCGGACCCATGAAAGGGGCGTGCGTGGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCA‑g‑gg                                                                                    <  1:32894/93‑1 (MQ=255)

   gACCGGACCCATGAAAGGGGCGTGCGTGGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCA‑g‑gg                                                                                    >  1:4675625/1‑93 (MQ=255)

   gACCGGACCCATGAAAGGGGCGTGCGTGGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGACGGCAATACTGGAGCA‑g‑gg                                                                                    >  1:154878/1‑93 (MQ=255)

    aCCGGACCCATGAAAGGGGCGTGCGTGGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCA‑g‑ggg                                                                                   <  1:2690648/93‑1 (MQ=255)

    aCCGGACCCATGAAAGGGGCGTGCGTGGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCA‑g‑ggg                                                                                   <  2:3854162/93‑1 (MQ=255)

     ccGGACCCATGAAAGGGGCGTGCGTGGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCA‑g‑gggg                                                                                  <  1:1179184/93‑2 (MQ=255)

     ccGGACACATGAAAGGGGCGTGCGTGGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCA‑g‑gggg                                                                                  >  2:1601187/1‑92 (MQ=255)

       ggACCCATGAAAGGGGCGTGCGTGGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCA‑g‑ggggaa                                                                                <  2:4494806/93‑4 (MQ=255)

       ggACCCATGAAAGGGGCGTGCGTGGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCA‑g‑ggggaa                                                                                >  1:484660/1‑90 (MQ=255)

        gACCCATGAAAGGGGCGTGCGTGGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCA‑g‑ggggaag                                                                               <  1:1140870/93‑5 (MQ=255)

        gACCCATGAAAGGGGCGTGCGTGGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCA‑g‑ggggaag                                                                               >  2:2228530/1‑89 (MQ=255)

           ccATGAAAGGGGCGTGCGTGGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGG‑GGGAAGTGa                                                                             <  1:3712401/93‑1 (MQ=255)

             aTGAAAGGGGCGTGCGTGGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGG‑GGGAAGTGAAc                                                                           <  1:4418761/93‑1 (MQ=255)

             aTGAAAGGGGCGTGCGTGGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGG‑GGGAAGTGAAc                                                                           >  1:3611060/1‑93 (MQ=255)

             aTGAAAGGGGCGTGCGTGGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGG‑GGGAAGTGAAc                                                                           >  1:3035921/1‑93 (MQ=255)

              tGAAAGGGGCGTGCGTGGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGG‑GGGAAGTGAACt                                                                          >  2:3707138/1‑93 (MQ=255)

              tGAAAGGGGCGTGCGTGGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGG‑GGGAAGTCAACt                                                                          >  2:3921700/1‑93 (MQ=255)

                aaaGGGGCGTGCGTGGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGG‑GGGAAGTGAACTTc                                                                        >  2:853156/1‑93 (MQ=255)

                 aaGGGGCGTGCGTGGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGG‑GGGAAGTGAACTTCa                                                                       <  2:2495088/93‑1 (MQ=255)

                  aGGGGCGTGCGTGGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGG‑GGGAAGTGAACTTCAt                                                                      <  2:936361/93‑1 (MQ=255)

                  aGGGGCGTGCGTGGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGG‑GGGAAGTGAACTTCAt                                                                      >  1:2374029/1‑93 (MQ=255)

                   ggggCGTGCGTGGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGG‑GGGAAGTGAACTTCAtt                                                                     <  1:2553900/93‑1 (MQ=255)

                   ggggCGTGCGTGGGCGCATGTCTGTTCGAAGGGTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGG‑GGGAAGTGAACTTCAtt                                                                     >  2:1120150/1‑93 (MQ=255)

                    gggCGTGCGTGGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGG‑GGGAAGTGAACTTCATTc                                                                    <  2:3834977/93‑1 (MQ=255)

                     ggCGTGCGTGGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGG‑GGGAAGTGAACTTCATTcc                                                                   <  2:2609063/93‑1 (MQ=255)

                      gcgtgcgtGGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGG‑GGGAAGTGAACTTCATTCCt                                                                  >  1:776842/1‑93 (MQ=255)

                         tgcgtgGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGG‑GGGAAGTGAACTTCATTCCTTGt                                                               <  1:3863012/93‑1 (MQ=255)

                          gcgtgGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGG‑GGGAAGTGAACTTCATTCCTTGTc                                                              <  2:4214871/93‑1 (MQ=255)

                              gggCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGG‑GGGAAGTGAACTTCATTCCTTGTCACCa                                                          >  1:2649171/1‑93 (MQ=255)

                              gggCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGG‑GGGAAGTGAACTTCATTCCTTGTCACCa                                                          <  1:1360722/93‑1 (MQ=255)

                                 cgcATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGG‑GGGAAGTGAACTTCATTCCTTGTCACCAtgt                                                       <  2:2905923/93‑1 (MQ=255)

                                  gcATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGG‑GGGAAGTGAACTTCATTCCTTGTCACCAtgtg                                                      >  2:2823347/1‑93 (MQ=255)

                                  gcATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGG‑GGGAAGTGAACTTCATTCCTTGTCACCAtgtg                                                      >  2:3018680/1‑93 (MQ=255)

                                  gcATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGATGGCAATACTGGAGCAGG‑GGGAAGTGAACTTCATTCCTTGTCACCAtgtg                                                      >  1:2509455/1‑93 (MQ=255)

                                    aTGTCTGTTCGAAGGTTGGGCGAAAGATGAGGCGCAGGCGCTGGCAATACTGGAGCAGG‑GGGAAGTGAACTTCATTCCTTGTCACCATGTGaa                                                    <  2:3118236/93‑1 (MQ=255)

                                    aTGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGG‑GGGAAGTGAACTTCATTCCTTGTCACCATGTGaa                                                    <  2:4658136/93‑1 (MQ=255)

                                    aTGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGG‑GGGAAGTGAACTTCATTCCTTGTCACCATGTGaa                                                    <  1:215496/93‑1 (MQ=255)

                                    aTGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGG‑GGGAAGTGAACTTCATTCCTTGTCACCATGTGaa                                                    >  2:2018212/1‑93 (MQ=255)

                                     tGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGG‑GGGAAGTGAACTTCATTCCTTGTCACCATGTGAAt                                                   <  1:4558241/93‑1 (MQ=255)

                                     tGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGG‑GGGAAGTGAACTTCATTCCTTGTCACCATGTGAAt                                                   >  1:2022460/1‑93 (MQ=255)

                                     tGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGG‑GGGAAGTGAACTTCATTCCCTGTCACCATGTGAAt                                                   >  2:1684417/1‑93 (MQ=255)

                                      gTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGG‑GGGAAGTGAACTTCATTCCTTGTCACCATGTGAATg                                                  >  2:1686543/1‑93 (MQ=255)

                                       tCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGG‑GGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGc                                                 >  1:4073006/1‑93 (MQ=255)

                                        cTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGG‑GGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGcc                                                <  1:875128/93‑1 (MQ=255)

                                         tGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGG‑GGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGcct                                               >  1:968184/1‑92 (MQ=255)

                                         tGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGG‑GGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCg                                               >  2:4406138/1‑93 (MQ=255)

                                          gTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGG‑GGGAAGTGAACTTCATTCCTTGTCACTATGTGAATGCCGt                                              <  2:4502154/93‑1 (MQ=255)

                                          gTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGG‑GGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGt                                              >  1:2675824/1‑93 (MQ=255)

                                           ttCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCGATACTGGAGCAGG‑GGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTc                                             <  1:1526268/93‑1 (MQ=255)

                                           ttCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGG‑GGGAAGTGAACTTCATTCCTTGTCACCCTGTGAATGCCGTc                                             >  2:1234333/1‑93 (MQ=255)

                                             cGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGG‑GGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCgg                                           >  2:4205710/1‑93 (MQ=255)

                                             cGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGG‑GGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCgg                                           >  1:430649/1‑93 (MQ=255)

                                              gAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGG‑GGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCggg                                          >  1:1668669/1‑93 (MQ=255)

                                               aaGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGG‑GGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGc                                         <  2:917635/93‑1 (MQ=255)

                                                aGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGG‑GGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGcc                                        >  1:1411877/1‑93 (MQ=255)

                                                  gTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGG‑GGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCaa                                      <  2:2931137/93‑1 (MQ=255)

                                                  gTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGG‑GGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCaa                                      <  2:1399580/93‑1 (MQ=255)

                                                  gTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGG‑GGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCaa                                      >  2:1082786/1‑93 (MQ=255)

                                                   ttGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGG‑GGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAAt                                     <  2:2521767/93‑1 (MQ=255)

                                                   ttGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGG‑GGGAAGAGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAAt                                     <  1:3307204/93‑1 (MQ=255)

                                                   ttGGGCGAAAGATAAAGCGCAGGCGCTGGCAATACTGGAGCAGG‑GGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAAt                                     <  2:3561225/93‑1 (MQ=255)

                                                    tGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGG‑GGGAAGTGAACTTCATTCCTTATCACCATGTGAATGCCGTCGGGCCAATg                                    <  1:3104548/93‑1 (MQ=255)

                                                     gggCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGG‑GGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATgg                                   >  1:2694762/1‑93 (MQ=255)

                                                     gggCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGG‑GGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATgg                                   <  2:4522760/93‑1 (MQ=255)

                                                      ggCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGG‑GGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATggg                                  <  2:3441329/93‑1 (MQ=255)

                                                         gAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGG‑GGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCgg                               >  1:129476/1‑93 (MQ=255)

                                                         gAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGG‑GGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCgg                               >  1:3073731/1‑93 (MQ=255)

                                                          aaaGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGG‑GGGCAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGt                              >  1:734260/1‑93 (MQ=255)

                                                            aGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGG‑GGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTAt                            <  1:2209429/93‑1 (MQ=255)

                                                             gATGAAGCGCAGGCGCTGGCAATACTGGAGCAGG‑GGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTAtt                           >  2:241172/1‑93 (MQ=255)

                                                              aTGAAGCGCAGGCGCTGGCAATACTGGAGCAGG‑GGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTATTa                          >  2:2438584/1‑93 (MQ=255)

                                                               tGAAGCGCAGGCGCTGGCAATACTGGAGCAGG‑GGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTATTAc                         >  2:2455318/1‑93 (MQ=255)

                                                                gAAGCGCAGGCGCTGGCAATACTGGAGCAGG‑GGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTATTACt                        <  1:1834418/93‑1 (MQ=255)

                                                                 aaGCGCAGGCGCTGGCAATACTGGAGCAGG‑GGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTATTACtt                       <  2:1124918/93‑1 (MQ=255)

                                                                 aaGCGCAGGCGCTGGCAATACTGGAGCAGG‑GGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTATTACtt                       <  1:3828565/93‑1 (MQ=255)

                                                                  aGCGCAGGCGCTGGCAATACTGGAGCAGG‑GGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTATTACTTc                      >  1:1011447/1‑93 (MQ=255)

                                                                  aGCGCAGGCGCTGGCAATACTGGAGCAGG‑GGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTATTACTTc                      <  1:3870940/93‑1 (MQ=255)

                                                                  aGCGCAGGCGCTGGCAATACTGGAGCAGG‑GGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTATTACTTc                      >  2:2370834/1‑93 (MQ=255)

                                                                     gcAGGCGCTGGCAATACTGGAGCAGG‑GGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTATTACTTCTGc                   >  1:1886030/1‑93 (MQ=255)

                                                                       aGGCGCTGGCAATACTGGAGCAGG‑GGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTATTACTTCTGCCa                 <  1:533394/93‑1 (MQ=255)

                                                                        ggCGCTGGCAATACTGGAGCAGG‑GGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTATTACTTCTGCCAg                >  2:3221032/1‑93 (MQ=255)

                                                                           gcTGGCAATACTGGAGCAGG‑GGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTATTACTTCTGCCAGTAt             <  2:2848327/93‑1 (MQ=255)

                                                                            cTGGCAATACTGGAGCA‑GCGGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTATTACTTCTGCCAGTATg            <  2:1819760/93‑1 (MQ=255)

                                                                             tGGCAATACTGGAGCAGG‑GGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTATTACTTCTGCCAGTATGc           <  2:317855/93‑1 (MQ=255)

                                                                             tGGCAATACTGGAGCAGG‑GGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTATTACTTCTGCCAGTATGc           >  2:2150140/1‑93 (MQ=255)

                                                                              ggCAATACTGGAGCAGG‑GGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTATTACTTCTGCCAGTATGcc          <  1:4323601/93‑1 (MQ=255)

                                                                              ggCAATACTGGAGCAGG‑GGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTATTACTTCTGCCAGTATGcc          <  2:425932/93‑1 (MQ=255)

                                                                                 aaTACTGGAGCAGG‑GGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTATTACTTCTGCCAGTATGCCGAt       >  2:1250056/1‑93 (MQ=255)

                                                                                  aTACTGGAGCAGG‑GGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTATTACTTCTGCCAGTATGCCGATg      >  2:3370284/1‑93 (MQ=255)

                                                                                   tACTGGAGCAGG‑GGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTATTACTTCTGCCAGTATGCCGATGc     >  2:2075080/1‑93 (MQ=255)

                                                                                   tACTGGAGCAGG‑GGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTATTACTTCTGCCAGTATGCCGATGc     >  1:1357004/1‑93 (MQ=255)

                                                                                   tACTGGAGCAGG‑GGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTATTACTTCTGCCAGTATGCCGATGc     <  1:1743963/93‑1 (MQ=255)

                                                                                   tACTGGAGCAGG‑GGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTATTACTTCTGCCAGTATGCCGATGc     <  1:3514410/93‑1 (MQ=255)

                                                                                    aCTGGAGCAGG‑GGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTATTACTTCTGCCAGTATGCCGATGCt    >  1:1857126/1‑93 (MQ=255)

                                                                                      tGGAGCAGG‑GGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTATTACTTCTGCCAGTATGCCGATGCtgg  >  2:1983288/1‑93 (MQ=255)

                                                                                      tGGAGCAGG‑GGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTATTACTTCTGCCAGTATGCCGATGCtgg  >  2:2220454/1‑93 (MQ=255)

GATGACCGGACCCATGAAAGGGGCGTGCGTGGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCA‑G‑GGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTATTACTTCTGCCAGTATGCCGATGCTGG  >  NC_000913/547739‑547916

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`