BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	115,968	+G	coding (1132/1386 nt)	hofB ←	HofB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	115,964	1	.	G	99.3%	455.2 / ‑4.0	144	coding (1136/1386 nt)	hofB	HofB
Reads supporting (aligned to +/- strand): ref base . (1/0); new base G (65/78); total (66/78)
Fisher's exact test for biased strand distribution p-value = 4.58e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.92e-01

Only 100 of 171 total aligned reads displayed.

GACGAATGACCGGCGTTATGGGCAGAACTTCAAATAAGGCCGTACGACCATAAAAACCGTGGTAGCAATGTACACAACCGGGTGCCTGCCAGT‑G‑GGG‑CAGCGGCGATGGCCATACATTGTCTGGAATGTGGATGGGCTCCCCTTGCTGCCGGCGACAATGTGGGCAAAGTTTGCGTACCA  >  NC_000913/115872‑116051

  cGAATGACCGGCGTTATGGGCAGAACTTCAAATAAGGCCGTACGACCATAAAAACCGTGGTAGCAATGTACACAACCGGGTGCCTGCCAGT‑g‑g                                                                                        <  1:4180178/93‑1 (MQ=255)

  cGAATGACCGGCGTTATGGGCAGAACTTCAAATAAGGCCGTACGACCATAAAAACCGTGGTAGCAATGTACACAACCGGGTGCCTGCCAGT‑g‑g                                                                                        <  1:2983465/93‑1 (MQ=255)

  cGAATGACCGGCGTTATGGGCAGAACTTCAAATAAGGCCGTACGACCATAAAAACCGTGGTAGCAATGTACACAACCGGGTGCCTGCCAGT‑g‑g                                                                                        <  2:3055986/93‑1 (MQ=255)

  cGAATGACCGGCGTTATGAGCAGAACTTCAAATAAGGCCGTACGACCATAAAACCCGTGGTAGCAATGTACACAACCGGGTGCCTGCCAGT‑g‑g                                                                                        <  1:2526889/93‑1 (MQ=255)

  cGAATGACCGGCGTTAGGGGCAGAACTTCAAATAAGGCCGTACGACCATAAAAACCGTGGTAGCAATGTACACTACCGGGTGCCGGCCAGT‑g‑g                                                                                        <  2:4558564/93‑1 (MQ=255)

   gAATGACCGGCGTTATGGGCAGAACTTCAAATAAGGCCGTACGACCATAAAAACCGTGGTAGCAATGTACACAACCGGGTGCCTGCCAGT‑g‑gg                                                                                       <  1:2858112/93‑1 (MQ=255)

    aaTGACCGGCGTTATGGGCAGAACTTCAAATAAGGCCGTACGACCATAAAAACCGTGGTAGCAATGTACACAACCGGGTGCCTGCCAGT‑g‑ggg                                                                                      >  1:4499308/1‑93 (MQ=255)

      tGACCGGCGTTATGGGCAGAACTTCAAATAAGGCCGTACGACCATAAAAACCGTGGTAGCAATGTACACAACCGGGTGCCTGCCAGT‑g‑ggggc                                                                                    <  1:801693/93‑3 (MQ=255)

      tGACCGGCGTTATGGGCAGAACTTCAAATAAGGCCGTACGACCATAAAAACCGTGGTAGCAATGTACACAACCGGGTGCCTGCCAGT‑g‑ggggc                                                                                    <  1:4321073/93‑3 (MQ=255)

      tGACCGGCGTTATGGGCAGAACTTCAAATAAGGCCGTACGACCATAAAAACCGTGGTAGCAATGTACACAACCGGGTGCCTGCCAGT‑g‑ggggc                                                                                    <  2:3244940/93‑3 (MQ=255)

          cGGCGTTATGGGCAGAACTTCAAATAAGGCCGTACGACCATAAAAACCGTGGTAGCAATGTACACAACCGGGTGCCTGCCAGTGG‑GGG‑CAgcg                                                                                <  2:2311300/93‑1 (MQ=255)

          cGGCGTTATGGGCAGAACTTCAAATAAGGCCGTACGACCATAAAAACCGTGGTAGCAATGTACACAACCGGGTGCCTGCCAGTGG‑GGG‑CAgcg                                                                                <  1:3905145/93‑1 (MQ=255)

          cGGCGTTATGGGCAGAACTTCAAATAAGGCCGTACGACCATAAAAACCGTGGTAGCAATGTACACAACCGGGTGCCTGCCAGTGG‑GGG‑CAgcg                                                                                >  1:3501918/1‑93 (MQ=255)

           ggCGTTATGGGCAGAACTTCAAATAAGGCCGTACGACCATAAAAACCGTGGTAGCAATGTACACAACCGGGTGCCTGCCAGTGG‑GGG‑CAgcgg                                                                               <  2:240424/93‑1 (MQ=255)

              gTTATGGGCAGAACTTCAAATAAGGCCGTACGACCATAAAAACCGTGGTAGCAATGTACACAACCGGGTGCCTGCCAGTGG‑GGG‑CAGCGGCGa                                                                            >  1:4646406/1‑93 (MQ=255)

              gTTATGGGCAGAACTTCAAATAAGGCCGTACGACCATAAAAACCGTGGTAGCAATGTACACAACCGGGTGCCTGCCAGTGG‑GGG‑CAGCGGCGa                                                                            <  2:2369008/93‑1 (MQ=255)

              gTTATGGGCAGAACTTCAAATAAGGCCGTACGACCATAAAAACCGTGGTAGCAATGTACACAACCGGGTGCCTGCCAGTGG‑GGG‑CAGCGGCGa                                                                            <  2:1889146/93‑1 (MQ=255)

              gTTATGGGCAGAACTTCAAATAAGGCCGTACGACCATAAAAACCGTGGTAGCAATGTACACAACCGGGTGCCTGCCAGTGG‑GGG‑CAGCGGCGa                                                                            <  2:1568253/93‑1 (MQ=255)

               ttATGGGCAGAACTTCAAATAAGGCCGTACGACCATAAAAACCGTGGTAGCAATGTACACAACCGGGTGCCTGCCAGTGG‑GGG‑CAGCGGCGAt                                                                           >  1:1860985/1‑93 (MQ=255)

                tATGGGCAGAACTTCAAATAAGGCCGTACGACCATAAAAACCGTGGTAGCAATGTACACAACCGGGTGCCTGCCAGTGG‑GGG‑CAGCGGCGATg                                                                          >  2:2672179/1‑93 (MQ=255)

                tATGGGCAGAACTTCAAATAAGGCCGTACGACCATAAAAACCGTGGTAGCAATGTACACAACCGGGTGCCTGCCAGTGG‑GGG‑CAGCGGCGATg                                                                          >  1:3522535/1‑93 (MQ=255)

                 aTGGGCAGAACTTCAAATAAGGCCGTACGACCATAAAAACCGTGGTAGCAATGTACACAACCGGGTGCCTGCCAGTGG‑GGG‑CAGCGGCGATgg                                                                         <  2:707488/93‑1 (MQ=255)

                     gCAGAACTTCAAATAAGGCCGTACGACCATAAAAACCGTGGTAGCAATGTACACAACCGGGTGCCTGCCAGTGG‑GGG‑CAGCGGCGATGGCCAt                                                                     <  2:693174/93‑1 (MQ=255)

                       aGAACTTCAAATAAGGCCGTACGACCATAAAAACCGTGGTAGCAATGTACACAACCGGGTGCCTGCCAGTGG‑GGG‑CAGCGGCGATGGCCATAc                                                                   >  1:2724707/1‑93 (MQ=255)

                        gAACTTCAAATAAGGCCGTACGACCATAAAAACCGTGGTAGCAATGTACACAACCGGGTGCCTGCCAGTGG‑GGG‑CAGCGGCGATGGCCATACa                                                                  >  2:1393112/1‑93 (MQ=255)

                        gAACTTCAAATAAGGCCGTACGACCATAAAAACCGTGGTAGCAATGTACACAACCGGGTGCCTGCCAGTGG‑GGG‑CAGCGGCGATGGCCATACa                                                                  >  1:4554564/1‑93 (MQ=255)

                         aaCTTCAAATAAGGCCGTACGACCATAAAAACCGTGGTAGCAATGTACACAACCGGGTGCCTGCCAGTGG‑GGG‑CAGCGGCGATGGCCATACAt                                                                 <  2:2792325/93‑1 (MQ=255)

                         aaCTTCAAATAAGGCCGTACGACCATAAAAACCGTGGTAGCAATGTACACAACCGGGTGCCTGCCAGTGG‑GGG‑CAGCGGCGATGGCCATACAt                                                                 >  2:3117456/1‑93 (MQ=255)

                          aCTTCAAATAAGGCCGTACGACCATAAAAACCGTGGTAGCAATGTACACAACCGGGTGCCTGCCAGTGG‑GGG‑CAGCGGCGATGGCCATACAtt                                                                <  1:2232005/93‑1 (MQ=255)

                          aCTTCAAATAAGGCCGTACGACCATAAAAACCGTGGTAGCAATGTACACAACCGGGTGCCTGCCAGTGG‑GGG‑CAGCGGCGATGGCCATACAtt                                                                >  1:299185/1‑93 (MQ=255)

                          aCTTCAAATAAGGCCGTACGACCATAAAAACCGTGGTAGCAATGTACACAACCGGGTGCCTGCCAGTGG‑GGG‑CAGCGGCGATGGCCATACAtt                                                                <  2:1736465/93‑1 (MQ=255)

                           cTTCAAATAAGGCCGTACGACCATAAAAACCGTGGTAGCAATGTACACAACCGGGTGCCTGCCAGTGG‑GGG‑CAGCGGCGATGGCCATACATTg                                                               <  2:4174331/93‑1 (MQ=255)

                            ttCAAATAAGGCCGTACGACCATAAAAACCGTGGTAGCAATGTACACAACCGGGTGCCTGCCAGTGG‑GGG‑CAGCGGCGATGGCCATACATTGt                                                              >  1:646043/1‑93 (MQ=255)

                            ttCAAATAAGGCCGTACGACCATAAAAACCGTGGTAGCAATGTACACAACCGGGTGCCTGCCAGTGG‑GGG‑CAGCGGCGATGGCCATACATTGt                                                              <  1:1170283/93‑1 (MQ=255)

                             tCAAATAAGGCCGTACGACCATAAAAACCGTGGTAGCAATGTACACAACCGGGTGCCTGCCAGTGG‑GGG‑CAGCGGCGATGGCCATACATTGTc                                                             <  1:2773125/93‑1 (MQ=255)

                             tCAAATAAGGCCGTACGACCATAAAAACCGTGGTAGCAATGTACACAACCGGGTGCCTGCCAGTGG‑GGG‑CAGCGGCGATGGCCATACATTGTc                                                             <  1:2393394/93‑1 (MQ=255)

                             tCAAATAAGGCCGTACGACCATAAAAACCGTGGTAGCAATGTACACAACCGGGTGCCTGCCAGTGG‑GGG‑CAGCGGCGATGGCCATACATTGTc                                                             >  1:4568758/1‑93 (MQ=255)

                              cAAATAAGGCCGTACGACCATAAAAACCGTGGTAGCAATGTACACAACCGGGTGCCTGCCAGTGG‑GGG‑CAGCGGCGATGGCCATACATTGTCt                                                            <  1:2760501/93‑1 (MQ=255)

                               aaaTAAGGCCGTACGACCATAAAAACCGTGGTAGCAATGTACACAACCGGGTGCCTGCCAGTGG‑GGG‑CAGCGGCGATGGCCATACATTGTCTg                                                           <  2:895292/93‑1 (MQ=255)

                               aaaTAAGGCCGTACGACCATAAAAACCGTGGTAGCAATGTACACAACCGGGTGCCTGCCAGTGG‑GGG‑CAGCGGCGATGGCCATACATTGTCTg                                                           >  1:1882427/1‑93 (MQ=255)

                               aaaTAAGGCCGTACGACCATAAAAACCGTGGTAGCAATGTACACAACCGGGTGCCTGCCAGTGG‑GGG‑CAGCGGCGATGGCCATACATTGTCTg                                                           >  2:3616420/1‑93 (MQ=255)

                                aaTAAGGCCGTACGACCATAAAAACCGTGGTAGCAATGTACACAACCGGGTGCCTGCCAGTGG‑GGG‑CAGCGGCGATGGCCATACATTGTCTgg                                                          >  1:1970322/1‑93 (MQ=255)

                                  tAAGGCCGTACGACCATAAAAACCGTGGTAGCAATGTACACAACCGGGTGCCTGCCAGTGG‑GGG‑CAGCGGCGATGGCCATACATTGTCTGGaa                                                        <  2:1817044/93‑1 (MQ=255)

                                   aaGGCCGTACGACCATAAAAACCGTGGTAGCAATGTACACAACCGGGTGCCTGCCAGTGG‑GGG‑CAGCGGCGATGGCCATACATTGTCTGGAAt                                                       <  1:1359161/93‑1 (MQ=255)

                                    aGGCCGTACGACCATAAAAACCGTGGTAGCAATGTACACAACCGGGTGCCTGCCAGTGG‑GGG‑CAGCGGCGATGGCCATACATTGTCTGGAAtg                                                      >  2:2498768/1‑93 (MQ=255)

                                     ggCGGTACGACCATAAAAACCGTGGTAGCAATGTACACAACCGGGTGCCTGCCAGTGG‑GGG‑CAGCGGCGATGGCCATACATTGTCTGGAAtgt                                                     <  2:2202674/93‑1 (MQ=255)

                                     ggCCGTACGACCATAAAAACCGTGGTAGCAATGTACACAACCGGGTGCCTGCCAGTGG‑GGG‑CAGCGGCGATGGCCATACATTGTCTGGAAtgt                                                     >  1:696225/1‑93 (MQ=255)

                                     ggCCGTACGACCATAAAAACCGTGGTAGCAATGTACACAACCGGGTGCCTGCCAGTGG‑GGG‑CAGCGGCGATGGCCATACATTGTCTGGAAtgg                                                     >  1:533517/1‑92 (MQ=255)

                                      gCCGTACGACCATAAAAACCGTGGTAGCAATGTACACAACCGGGTGCCTGCCAGTGG‑GGG‑CAGCGGCGATGGCCATACATTGTCTGGAAtgtg                                                    <  1:1921533/93‑1 (MQ=255)

                                      gCCGTACGACCATAAAAACCGTGGTAGCAATGTACACAACCGGGTGCCTGCCAGTGG‑GGG‑CAGCGGCGATGGCCATACATTGTCTGGAAtgtg                                                    >  1:1801683/1‑93 (MQ=255)

                                        cGTACGACCATAAAAACCGTGGTAGCAATGTACACAACCGGGTGCCTGCCAGTGG‑GGG‑CAGCGGCGATGGCCATACATTGTCTGGAATGTGGa                                                  >  1:2258449/1‑93 (MQ=255)

                                         gTACGACCATAAAAACCGTGGTAGCAATGTACACAACCGGGTGCCTGCCAGTGG‑GGG‑CAGCGGCGATGGCCATACATTGTCTGGAATGTGGAt                                                 <  1:947340/93‑1 (MQ=255)

                                         gTACGACCATAAAAACCGTGGTAGCAATGTACACAACCGGGTGCCTGCCAGTGG‑GGG‑CAGCGGCGATGGCCATACATTGTCTGGAATGTGGAt                                                 >  2:1993714/1‑93 (MQ=255)

                                         gTACGACCATAAAAACCGTGGTAGCAATGTACACAACCGGGTGCCTGCCAGTGG‑GGG‑CAGCGGCGATGGCCATACATTGTCTGGAATGTGGAt                                                 <  1:2812041/93‑1 (MQ=255)

                                         gTACGACCATAAAAACCGTGGTAGCAATGTACACAACCGGGTGCCTGCCAGT‑GCGGG‑CAGCGGCGATGGCCATACATTGTCTGGAATGTGGAt                                                 >  2:437399/1‑93 (MQ=255)

                                          tACGACCATAAAAACCGTGGTAGCAATGTACACAACCGGGTGCCTGCCAGTGG‑GGG‑CAGCGGCGATGGCCATACATTGTCTGGAATGTGGATg                                                <  2:686702/93‑1 (MQ=255)

                                           aCGACCATAAAAACCGTGGTAGCAATGTACACAACCGGGTGCCTGCCAGTGG‑GGG‑CAGCGGCGATGGCCATACATTGTCTGGAATGTGGATgg                                               <  1:4479808/93‑1 (MQ=255)

                                            cGACCATAAAAACCGTGGTAGCAATGTACACAACCGGGTGCCTGCCAGTGG‑GGG‑CAGCGGCGATGGCCATACATTGTCTGGAATGTGGATggg                                              >  2:171832/1‑93 (MQ=255)

                                            cGACCATAAAAACCGTGGTAGCAATGTACACAACCGGGTGCCTGCCAGTGG‑GGG‑CAGCGGCGATGGCCATACATTGTCTGGAATGTGGATggg                                              >  1:1612307/1‑93 (MQ=255)

                                             gACCATAAAAACCGTGGTAGCAATGTACACAACCGGGTGCCTGCCAGTGG‑GGG‑CAGCGGCGATGGCCATACATTGTCTGGAATGTGGATGGGc                                             >  2:1173937/1‑93 (MQ=255)

                                             gACCATAAAAACCGTGGTAGCAATGTACACAACCGGGTGCCTGCCAGTGG‑GGG‑CAGCGGCGATGGCCATACATTGTCTGGAATGTGGATGGGc                                             <  2:2927012/93‑1 (MQ=255)

                                              aCCATAAAAACCGTGGTAGCAATGTACACAACCGGGTGCCTGCCAGTGG‑GGG‑CAGCGGCGATGGCCATACATTGTCTGGAATGTGGATGGGCt                                            <  1:2705498/93‑1 (MQ=255)

                                               ccATAAAGCCCGTGGTAGCAATGTACACAGCCGGTTGCCTGCCAGTGG‑GGG‑CAGCGGCGATGTCCATACATTGTCTGGAATGTGGATGGGCTc                                           <  2:4630272/93‑1 (MQ=255)

                                                  tAAAAACCGTGGTAGCAATGTACACAACCGGGTGCCTGCCAGTGG‑GGG‑CAGCGGCGATGGCCATACATTGTCTGGAATGTGGATGGGCTcccc                                        <  1:2445489/93‑1 (MQ=255)

                                                  tAAAAACCGTGGTAGCAATGTACACAACCGGGTGCCTGCCAGTGG‑GGG‑CAGCGGCGATGGCCATACATTGTCTGGAATGTGGATGGGCTcccc                                        >  1:2468671/1‑93 (MQ=255)

                                                  tAAAAACCGTGGTAGCAATGTACACAACCGGGTGCCTGCCAGTGG‑GGG‑CAGCGGCGATGGCCATACATTGTCTGGAATGTGGATGGGCTcccc                                        >  1:40289/1‑93 (MQ=255)

                                                  tAAAAACCGTGGTAGCAATGTACACAACCGGGTGCCTGCCAGTGG‑GGG‑CAGCGGCGATGGCCATACATTGTCTGGAATGTGGATGGGCTcccc                                        >  2:29116/1‑93 (MQ=255)

                                                  tAAAAACCGTGGTAGCAATGTACACAACCGGGTGCCTGCCAGTGG‑GGG‑CAGCGGCGATGGCCATACATTGTCTGGAATGTGGATGGGCTcccc                                        <  1:1713098/93‑1 (MQ=255)

                                                    aaaaCCGTGGTAGCAATGTACACAACCGGGTGCCTGCCAGTGG‑GGG‑CAGCGGCGATGGCCATACATTGTCTGGAATGTGGATGGGCTCCCCtt                                      >  2:1037414/1‑93 (MQ=255)

                                                    aaaaCCGTGGTAGCAATGTACACAACCGGGTGCCTGCCAGTGG‑GGG‑CAGCGGCGATGGCCATACATTGTCTGGAATGTGGATGGGCTCCCCtt                                      <  1:1011096/93‑1 (MQ=255)

                                                     aaaCCGTGGTAGCAATGTACACAACCGGGTGCCTGCCAGTGG‑GGG‑CAGCGGCGATGGCCATACATTGTCTGGAATGTGGATGGGCTCCCCTtg                                     >  2:2165240/1‑93 (MQ=255)

                                                      aaCCGTGGTAGCAATGTACACAACCGGGTGCCTGCCAGTGG‑GGG‑CAGCGGCGATGGCCATACATTGTCTGGAATGTGGATGGGCTCCCCTtgc                                    <  1:3136512/93‑1 (MQ=255)

                                                      aaCCGTGGTAGCAATGTACACAACCGGGTGCCTGCCAGTGG‑GGG‑CAGCGGCGATGGCCATACATTGTCTGGAATGTGGATGGGCTCCCCTtgc                                    <  2:1445559/93‑1 (MQ=255)

                                                       aCCGTGGTAGCAATGTACACAACCGGGTGCCTGCCAGTGG‑GGG‑CAGCGGCGATGGCCATACATTGTCTGGAATGTGGATGGGCTCCCCTtgct                                   <  1:2610068/93‑1 (MQ=255)

                                                       aCCGTGGTAGCAATGTACACAACCGGGTGCCTGCCAGTGG‑GGG‑CAGCGGCGATGGCCATACATTGTCTGGAATGTGGATGGGCTCCCCTtgct                                   >  2:1707346/1‑93 (MQ=255)

                                                       aCCGTGGTAGCAATGTACACAACCGGGTGCCTGCCAGTGG‑GGG‑CAGCGGCGATGGCCATACATTGTCTGGAATGCGGATGGGCTCCCCTtgct                                   >  2:1147181/1‑93 (MQ=255)

                                                          gTGGTAGCAATGTACACAACCGGGTGCCTGCCAGTGG‑GGG‑CAGCGGCGATGGCCATACATTGTCTGGAATGTGGATGGGCTCCCCTTGCTGcc                                >  1:3229822/1‑93 (MQ=255)

                                                           gggTAGCAATGTACACAACCGGGTGCCTGCCAGTGG‑GGG‑CAGCGGCGATGGCCATACATTGTCTGGAATGTGGATGGGCTCCCCTTGCTGCCg                               <  2:2347088/92‑1 (MQ=255)

                                                            ggTAGCAATGTACACAACCGGGTGCCTGCCAGTGG‑GGG‑CAGCGGCGATGGCCATACATTGTCTGGAATGTGGATGGGCTCCCCTTGCTGCCgg                              >  1:1354444/1‑93 (MQ=255)

                                                             gTAGCAATGTACACAACCGGGTGCCTGCCAGTGG‑GGG‑CAGCGGCGATGGCCATACATTGTCTGGAATGTGGATGGGCTCCCCTTGCTGCCGGc                             <  2:2111947/93‑1 (MQ=255)

                                                               aGCAATGTACACAACCGGGTGCCTGCCAGTGG‑GGG‑CAGCGGCGATGGCCATACATTGTCTGGAATGTGGATGGGCTCCCCTTGCTGCCGGCGa                           >  1:1033710/1‑93 (MQ=255)

                                                                  aaTGTACACAACCGGGTGCCTGCCAGTGG‑GGG‑CAGCGGCGATGGCCATACATTGTCTGGAATGTGGATGGGCTCCCCTTGCTGCCGGCGACaa                        >  1:1505891/1‑93 (MQ=255)

                                                                  aaTGTACACAACCGGGTGCCTGCCAGTGG‑GGG‑CAGCGGCGATGGCCATACATTGTCTGGAATGTGGATGGGCTCCCCTTGCTGCCGGCGACaa                        <  1:1825306/93‑1 (MQ=255)

                                                                   aTGTACACAACCGGGTGCCTGCCAGTGG‑GGG‑CAGCGGCGATGGCCATACATTGTCTGGAATGTGGATGGGCTCCCCTTGCTGCCGGCGACAAt                       >  1:3947893/1‑93 (MQ=255)

                                                                    tGTACACAACCGGGTGCCTGCCAGTGG‑GGG‑CAGCGGCGATGGCCATACATTGTCTGGAATGTGGATGGGCTCCCCTTGCTGCCGGCGACAAtg                      <  2:1302769/93‑1 (MQ=255)

                                                                     gTACACAACCGGGTGCCTGCCAGTGG‑GGG‑CAGCGGCGATGGCCATACATTGTCTGGAATGTGGATGGGCTCCCCTTGCTGCCGGCGACAAtgt                     <  1:2718067/93‑1 (MQ=255)

                                                                     gTACACAACCGGGTGCCTGCCAGTGG‑GGG‑CAGCGGCGATGGCCATACATTGTCTGGAATGTGGATGGGCTCCCCTTGCTGCCGGCGACAAtgt                     <  1:1686156/93‑1 (MQ=255)

                                                                       acacAACCGGGTGGCTGCCAGTGG‑GGG‑CAGCGGCGATGGCCATACATTGTCTGGCATGTGGATGGGCTCCCCTTGCTGCCGGCGACAATGTgg                   >  2:2701610/1‑93 (MQ=255)

                                                                         acaACCGGGTGCCTGCCAGTGG‑GGG‑CAGCGGCGATGGCCATACATTGTCTGGAATGTGGATGGGCTCCCCTTGCTGCCGGCGACAATGTGGGc                 <  2:3752292/93‑1 (MQ=255)

                                                                         acaACCGGGTGCCTGCCAGTGG‑GGG‑CAGCGGCGATGGCCATACATTGTCTGGAATGTGGATGGGCTCCCCTTGCTGCCGGCGACAATGTGGGc                 >  2:1087110/1‑93 (MQ=255)

                                                                            aCCGGGTGCCTGCCAGTGG‑GGG‑CAGCGGCGATGGCCATACATTGTCTGGAATGTGGATGGGCTCCCCTTGCTGCCGGCGACAATGTGGGCaaa              <  2:1527014/93‑1 (MQ=255)

                                                                            aCCGGGTGCCTGCCAGTGG‑GGG‑CAGCGGCGATGGCCATACATTGTCTGGAATGTGGATGGGCTCCCCTTGCTGCCGGCGACAATGTGGGCaaa              <  2:1846204/93‑1 (MQ=255)

                                                                                ggTGCCTGCCAGTGG‑GGG‑CAGCGGCGATGGCCATACATTGTCTGGAATGTGGATGGGCTCCCCTTGCTGCCGGCGACAATGTGGGCAAAGttt          <  1:3406557/93‑1 (MQ=255)

                                                                                   gcctgccAGTGG‑GGG‑CAGCGGCGATGGCCATACATTGTCTGGAATGTGGATGGGCTCCCCTTGCTGCCGGCGACAATGTGGGCAAAGTTTGCg       >  1:3727087/1‑93 (MQ=255)

                                                                                   gcctgccAGTGG‑GGG‑CAGCGGCGATGGCCATACATTGTCTGGAATGTGGATGGGCTCCCCTTGCTGCCGGCGACAATGTGGGCAAAGTTTGCg       >  1:1449851/1‑93 (MQ=255)

                                                                                   gcctgccAGTGG‑GGG‑CAGCGGCGATGGCCATACATTGTCTGGAATGTGGATGGGCTCCCCTTGCTGCCGGCGACAATGTGGGCAAAGTTTGCg       <  1:1998663/93‑1 (MQ=255)

                                                                                    cctgccAGTGG‑GGG‑CAGCGGCGATGGCCATACATTGTCTGGAATGTGGATGGGCTCCCCTTGCTGCCGGCGACAATGTGGGCAAAGTTTGCGt      <  2:3033142/93‑1 (MQ=255)

                                                                                     ctgctAGT‑G‑GGGTCAGCGGCGATGTCCATACATTGTCTGGAATGTGGATGGGCTCCCCTTGCTGCCGGCGACAATGTGGGCAAAGTTTGCGTa     <  2:4534866/93‑1 (MQ=255)

                                                                                     ctgccAGTGG‑GGG‑CAGCGGCGATGGCCATACATTGTCTGGAATGTGGATGGGCTCCCCTTGCTGCCGGCGACAATGTGGGCAAAGTTTGCGTa     <  1:3071966/93‑1 (MQ=255)

                                                                                        ccAGTGG‑GGG‑CAGCGGCGATGGCCATACATTGTCTGGAATGTGGATGGGCTCCCCTTGCTGCCGGCGACAATGTGGGCAAAGTTTGCGTACCa  <  2:2612871/93‑1 (MQ=255)

GACGAATGACCGGCGTTATGGGCAGAACTTCAAATAAGGCCGTACGACCATAAAAACCGTGGTAGCAATGTACACAACCGGGTGCCTGCCAGT‑G‑GGG‑CAGCGGCGATGGCCATACATTGTCTGGAATGTGGATGGGCTCCCCTTGCTGCCGGCGACAATGTGGGCAAAGTTTGCGTACCA  >  NC_000913/115872‑116051

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 19 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`