Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,751,646	(G)5→6	coding (209/2103 nt)	b1673 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,751,641	1	.	G	95.5%	262.3 / 5.7	88	coding (214/2103 nt)	b1673	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base . (1/3);  new base G (39/45);  total (40/48)
Fisher's exact test for biased strand distribution p-value = 6.23e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.28e-01

TTGCGCTGCAAAAAAGCCACCCATATGGGCATCGACGACTAAATTTCCTTTGGTAAAAGTAGAAAGTGAGGTGATATTTACGCGAGAACTACA‑GGGGGCACCAGATCCAGTTAATGGGCCGGTAGCAAAGACTAATTTATTCGCTTCATCGAAAGGTTTCGTGCCTGGCGGTACTTCGTCAT  >  NC_000913/1751549‑1751730                                                                                              |                                                                                          ttGCGCTGCAAAAAAGCCACCCATATGGGCATCGACGACTAAATTTCCTTTGGTAAAAGTAGAAAGTGAGGTGATATTTACGCGAGAACTACa                                                                                            >  1:1212909/1‑93 (MQ=255)  tGCGCTGCAAAAAAGCCACCCATATGGGCATCGACGACTAAATTTCCTTTGGTAAAAGTAGAAAGTGAGGTGATATTTACGCGAGAACTACA‑g                                                                                          <  1:547606/93‑1 (MQ=255)   gcgcTGCAAAAAAGCCACCCATATGGGCATCGACGACTAAATTTCCTTTGGTAAAAGTAGAAAGTGAGGTGATATTTACGCGAGAACTACA‑gg                                                                                         <  1:3630212/93‑1 (MQ=255)   gcgcTGCAAAAAAGCCACCCATATGGGCATCGACGACTAAATTTCCTTTGGTAAAAGTAGAAAGTGAGGTGATATTTACGCGAGAACTACA‑gg                                                                                         <  2:4421056/93‑1 (MQ=255)   gcgcTGCAAAAAAGCCACCCATATGGGCATCGACGACTAAATTTCCTTTGGTAAAAGTAGAAAGTGAGGTGATATTTACGCGAGAACTACA‑gg                                                                                         >  2:561738/1‑93 (MQ=255)   gcgcTACAAAAAAGCCACCCATATGGCCATCGACGACTAAATTTCCTTTGGTAAAAGTAGAAAGTGAGGTGATATTTACGCGAGAACTACA‑gg                                                                                         <  2:1858505/93‑1 (MQ=255)    cgcTGCAAAAAAGCCACCCATATGGGCATCGACGACTAAATTTCCTTTGGTAAAAGTAGAAAGTGAGGTGATATTTACGCGAGAACTACA‑ggg                                                                                        <  1:106112/93‑1 (MQ=255)     gcTGCAAAAAAGCCACCCATATGGGCATCGACGACTAAATTTCCTTTGGTAAAAGTAGAAAGTGAGGTGATATTTACGCGAGAACTACA‑gggg                                                                                       <  2:2836576/93‑1 (MQ=255)     gcTGCAAAAAAGCCACCCATATGGGCATCGACGACTAAATTTCCTTTGGTAAAAGTAGAAAGTGAGGTGATATTTACGCGAGAACTACA‑gggg                                                                                       <  1:2737003/93‑1 (MQ=255)        gCAAAAAAGCCACCCATATGGGCATCGACGACTAAATTTCCTTTGGTAAAAGTAGAAAGTGAGGTGATATTTACGCGAGAACTACA‑ggggggc                                                                                    >  1:2104425/1‑91 (MQ=255)            aaaaGCCACCCATATGGGCATCGACGACTAAATTTCCTTTGGTAAAAGTAGAAAGTGAGGTGATATTTACGCGAGAACTACAGGGGGGCACCa                                                                                 >  2:2208328/1‑93 (MQ=255)             aaaGCCACCCATATGGGCATCGACGACTAAATTTCCTTTGGTAAAAGTAGAAAGTGAGGTGATATTTACGCGAGAACTACA‑GGGGGCACCAGa                                                                               <  1:98414/93‑1 (MQ=255)              aaGCCACCCATATGGGCATCGACGACTAAATTTCCTTTGGTAAAAGTAGAAAGTGAGGTGATATTTACGCGAGAACTACAGGGGGGCACCAGa                                                                               <  2:3492844/93‑1 (MQ=255)                 ccACCCATATGGGCATCGACGACTAAATTTTCTTTGGTAAAAGTAGAAAGTGAGGTGATATTTACGCGAGAACTACAGGGGGGCACCAGATcc                                                                            >  2:4439378/1‑93 (MQ=255)                 ccACCCATATGGGCATCGACGACTAAATTTCCTTTGGTAAAAGTAGAAAGTGAGGTGATATTTACGCGAGAACTACAGGGGGGCACCAGATcc                                                                            >  2:3510267/1‑93 (MQ=255)                  cACCCATATGGGCATCGACGACTAAATTTCCTTTGGTAAAAGTAGAAAGTGAGGTGATATTTACGCGAGAACTACAGGGGGGCACCAGATCCa                                                                           >  2:2033865/1‑93 (MQ=255)                  cACCCATATGGGCATCGACGACTAAATTTCCTTTGGTAAAAGTAGAAAGTGAGGTGATATTTACGCGAGAACTACAGGGGGGCACCAGATCCa                                                                           <  2:381376/93‑1 (MQ=255)                   aCCCATATGGGCATCGACGACTAAATTTCCTTTGGTAAAAGTAGAAAGTGAGGTGATATTTACGCGAGAACTACAGGGGGGCACCAGCTCCAg                                                                          >  2:4141116/1‑93 (MQ=255)                   aCCCATATGGGCATCGACGACTAAATTTCCTTTGGTAAAAGTAGAAAGTGAGGTGATATTTACGCGAGAACTACAGGGGGGCACCAGATCCAg                                                                          >  1:963692/1‑93 (MQ=255)                    cccATATGGGCATCGACGACTAAATTTCCTTTGGTAAAAGTAGAAAGTGAGGTGATATTTACGCGAGAACTACAGGGGGGCACCAGATCCAGt                                                                         >  2:4664167/1‑93 (MQ=255)                      cATATGGGCATCGACGACTAAATTTCCTTTGGTAAAAGTAGAAAGTGAGGTGATATTTACGCGAGAACTACAGGGGGGCACCAGATCCAGTTa                                                                       >  2:1099077/1‑93 (MQ=255)                       atatGGGCATCGACGACTAAATTTCCTTTGGTAAAAGTAGAAAGTGAGGTGATATTTACGCGAGAACTACAGGGGGGCACCAGATCCAGTTat                                                                      >  1:1431346/1‑92 (MQ=255)                        tatGGGCATCGACGACTAAATTTCCTTTGGTAAAAGTAGAAAGTGAGGTGATATTTACGCGAGAACTACAGGGGGGCACCAGATCCAGTTAAt                                                                     <  2:1817201/93‑1 (MQ=255)                         atGGGCATCGACGACTAAATTTCCTTTGGTAAAAGTAGAAAGTGAGGTGATATTTACGCGAGAACTACAGGGGGGCACCAGATCCAGTTAATg                                                                    <  1:4275037/93‑1 (MQ=255)                          tGGGCATCGACGACTAAATTTCCTTTGGTAAAAGTAGAAAGTGAGGTGATATTTACGCGAGAACTACAGGGGGGCACCAGATCCAGTTAATgg                                                                   <  2:1844383/93‑1 (MQ=255)                          tGGGCATCGACGACTAAATTTCCTTTGGTAAAAGTAGAAAGTGAGGTGATATTTACGCGAGAACTACAGGGGGGCACCAGATCCAGTTAATgg                                                                   <  2:3014835/93‑1 (MQ=255)                               aTCGACGACTAAATTTCCTTTGGTAAAAGTAGAAAGTGAGGTGATATTTACGCGAGAACTACAGGGGGGCACCAGATCCAGTTAATGGGCCgg                                                              >  2:3540289/1‑93 (MQ=255)                                tCGACGACTAAATTTCCTTTGGTAAAAGTAGAAAGTGAGGTGATATTTACGCGAGAACTACAGGGGGGCACCAGATCCAGTTAATGGGCCGGt                                                             >  2:1951628/1‑93 (MQ=255)                                tCGACGACTAAATTTCCTTTGGTAAAAGTAGAAAGTGAGGTGATATTTACGCGAGAACTACAGGGGGGCACCAGATCCAGTTAATGGGCCGGt                                                             >  2:3038512/1‑93 (MQ=255)                                tCGACGACTAAATTTCCTTTGGTAAAAGTAGAAAGTGAGGTGATATTTACGCGAGAACTACAGGGGGGCACCAGATCCAGTTAATGGGCCGGt                                                             >  1:226639/1‑93 (MQ=255)                                 cgacgaCTAAATTTCCTTTGGTAAAAGTAGAAAGTGAGGTGATATTTACGCGAGAACTACAGGGGGGCACCAGATCCAGTTAATGGGCCGGTa                                                            <  2:2568822/93‑1 (MQ=255)                                  gacgacTAAATTTCCTTTGGTAAAAGTAGAAAGTGAGGTGATATTTACGCGAGAACTACAGGGGGGCACCAGATCCAGTTAATGGGCCGGTAg                                                           <  1:2225601/93‑1 (MQ=255)                                  gacgacTAAATTTCCTTTGGTAAAAGTAGAAAGTGAGGTGATATTTACGCGAGAACTACAGGGGGGCACCAGATCCAGTTAATGGGCCGGTAg                                                           >  1:302566/1‑93 (MQ=255)                                   acgacTAAATTTCCTTTGGTAAAAGTAGAAAGTGAGGTGATATTTACGCGAGAACTACAGGGGGGCACCAGATCCAGTTAATGGGCCGGTAGc                                                          >  1:3392233/1‑93 (MQ=255)                                   acgacTAAATTTCCTTTGGTAAAAGTAGAAAGTGAGGTGATATTTACGCGAGAACTACAGGGGGGCACCAGATCCAGTTAATGGGCCGGTAGc                                                          >  1:4324855/1‑93 (MQ=255)                                   acgacTAAATTGCCTTTGGTAAAAGTAGAAAGTGAGGTGATATTTACGCGAGAACTACAGGGGGGCACCAGATCCAGTTAATGGGCCGGTAGc                                                          <  2:1162606/93‑1 (MQ=255)                                     gacTAAATTTCCTTTGGTAAAAGTAGAAAGTGAGGTGATATTTACGCGAGAACTACAGGGGGGCACCAGATCCAGTTAATGGGCCGGTAGCaa                                                        <  2:1453683/93‑1 (MQ=255)                                     gacTAAATTTCCTTTGGTAAAAGTAGAAAGTGAGGTGATATTTACGCGAGAACTACAGGGGGGCACCAGATCCAGTTAATGGGCCGGTAGCaa                                                        >  1:999175/1‑93 (MQ=255)                                     gacTAAATTTCCTTTGGTAAAAGTAGAAAGTGAGGTGATATTTACGCGAGAACTACAGGGGGGCACCAGATCCAGTTAATGGGCCGGTAGCaa                                                        >  1:2231533/1‑93 (MQ=255)                                       cTAAATTTCCTTTGGTAAAAGTAGAAAGTGAGGTGATATTTACGCGAGAACTACAGGGGGGCACCAGATCCAGTTAATGGGCCGGTAGCAAAg                                                      <  1:2238621/93‑1 (MQ=255)                                        tAAATTTCCTTTGGTAAAAGTAGAAAGTGAGGTGATATTTACGCGAGAACTACAGGGGGGCACCAGATCCAGTTAATGGGCCGGTAGCAAAGa                                                     <  1:2214184/93‑1 (MQ=255)                                        tAAATTTCCTTTGGTAAAAGTAGAAAGTGAGGTGATATTTACGCGAGAACTACAGGGGGGCACCAGATCCAGTTAATGGGCCGGTAGCAAAGa                                                     >  1:2873637/1‑93 (MQ=255)                                         caaTTTCCTTGGGTAAAAGTAGAAATTGAGGTGATATTTGCGCGAGAACTACAGGGGGGCCCGAGATCCGGTTAATGGGCCGGTCGCAAacac                                                    <  2:1785373/92‑1 (MQ=255)                                          aaTTTCCTTTGGTAAAAGTAGAAAGTGAGGTGATATTTACGCGAGAACTACAGGGGGGCACCAGATCCAGTTAATGGGCCGGTAGCAAAGACt                                                   >  1:1530859/1‑93 (MQ=255)                                            tttCCTTTGGTAAAAGTAGAAAGTGAGGTGATATTTACGCGAGAACTACAGGGGGGCACCAGATCCAGTTAATGGGCCGGTAGCAAAGACTaa                                                 <  2:2852393/93‑1 (MQ=255)                                               ccTTTGGTAAAAGTAGAAAGTGAGGTGATATTTACGCGAGAACTACAGGGGGGCACCAGATCCAGTTAATGGGCCGGTAGCAAAGACTAAttt                                              >  2:4131437/1‑93 (MQ=255)                                                cTTTGGTAAAAGTAGAAAGTGAGGTGATATTTACGCGAGAACTACAGGGGGGCACCAGATCCATTTAATGGGCCGGTAGCAAAGACTAATTTa                                             <  1:3256944/93‑1 (MQ=255)                                                cTTTGGTAAAAGTAGAAAGTGAGGTGATATTTACGCGAGAACTACAGGGGGGCACCAGATCCAGTTAATGGGCCGGTAGCAAAGACTAATTTa                                             <  1:2474211/93‑1 (MQ=255)                                                cTTTGGTAAAAGTAGAAAGTGAGGTGATATTTACGCGAGAACTACAGGGGGGCACCAGATCCAGTTAATGGGCCGGTAGCAAAGACTAATTTa                                             <  2:2343251/93‑1 (MQ=255)                                                cTTTGGTAAAAGTAGAAAGTGAGGTAATATTTACGCGAGAACTACAGGGGGGCACCAGATCCAGTTAATGGGCCGGTAGCAAAGACTAATTTa                                             >  2:2800313/1‑93 (MQ=255)                                                 tttGGTAAAAGTAGAAAGTGAGGTGATATTTACGCGAGAACTACAGGGGGGCACCAGATCCAGTTAATGGGCCGGTAGCAAAGACTAATTTAt                                            >  1:2567742/1‑93 (MQ=255)                                                  ttGGTAAAAGTAGAAAGTGAGGTGATATTTACGCGAGAACTACAGGGGGGCACCAGATCCAGTTAATGGGCCGGTAGCAAAGACTAATTTAtt                                           >  1:99635/1‑93 (MQ=255)                                                    ggTAAAAGTAGAAAGTGAGGTGATATTTACGCGAGAACTACAGGGGGGCACCAGATCCAGTTAATGGGCCGGTAGCAAAGACTAATTTATTCg                                         >  1:324766/1‑93 (MQ=255)                                                     gTAAAAGTAGAAAGTGAGGTGATATTTACGCGAGAACTACAGGGGGGCACCAGATCCAGTTAATGGGCCGGTAGCAAAGACTAATTTATTCGc                                        <  2:109180/93‑1 (MQ=255)                                                     gTAAAAGTAGAAAGTGAGGTGATATTTACGCGAGAACTACAGGGGGGCACCAGATCCAGTTAATGGGCCGGTAGCAAAGACTAATTTATTCGc                                        <  1:3484767/93‑1 (MQ=255)                                                       aaaaGTAGAAAGTGAGGTGATATTTACGCGAGAACTACAGGGGGGCACCAGATCCAGTTAATGGGCCGGTAGCAAAGACTAATTTATTCGCtt                                      <  2:1964866/93‑1 (MQ=255)                                                        aaaGTAGAAAGTGAGGTGATATTTACGCGAGAACTACAGGGGGGCACCAGATCCAGTTAATGGGCCGGTAGCAAAGACTAATTTATTCGCTTc                                     <  2:1904134/93‑1 (MQ=255)                                                         aaGTAGAAAGTGAGGTGATATTTACGCGAGAACTACAGGGGGGCACCAGATCCAGTTAATGGGCCGGTAGCAAAGACTAATTTATTCGCTTCa                                    <  2:2955371/93‑1 (MQ=255)                                                         aaGTAGAAAGTGAGGTGATATTTACGCGAGAACTACAGGGGGGCACCAGATCCAGTTAATGGGCCGGTAGCAAAGACTAATTTATTCGCTTCa                                    <  1:514834/93‑1 (MQ=255)                                                         aaGTAGAAAGTGAGGTGATATTTACGCGAGAACTACAGGGGGGCACCAGATCCAGTTAATGGGCCGGTAGCAAAGACTAATTTATTCGCTTCa                                    <  2:1846647/93‑1 (MQ=255)                                                         aaGTAGAAAGTGAGGTGATATTTACGCGAGAACTACAGGGGGGCACCAGATCCAGTTAATGGGCCGGTAGCAAAGACTAATTTATTCGCTTCa                                    <  1:4644183/93‑1 (MQ=255)                                                          aGTAGAAAGTGAGGTGATATTTACGCGAGAACTACAGGGGGGCACCAGATCCAGTTAATGGGCCGGTAGCAAAGACTAATTTATTCGCTTCAt                                   >  1:4147793/1‑93 (MQ=255)                                                           gTAGAAAGTGAGGTGATATTTACGCGAGAACTACAGGGGGGCACCAGATCCAGTTAATGGGCCGGTAGCAAAGACTAATTTATTCGCTTCATc                                  <  2:351387/93‑1 (MQ=255)                                                           gTAGAAAGTGAGGTGATATTTACGCGAGAACTACAGGGGGGCACCAGATCCAGTTAATGGGCCGGTAGCAAAGACTAATTTATTCGCTTCATc                                  >  2:3531933/1‑93 (MQ=255)                                                             aGAAAGTGAGGTGATATTTACGCGAGAACTACAGGGGGGCACCAGATCCAGTTAATGGGCCGGTAGCAAAGACTAATTTATTCGCTTCATCGa                                >  1:2761171/1‑93 (MQ=255)                                                             aGAAAGTGAGGTGATATTTACGCGAGAACTACAGGGGGGCACCAGATCCAGTTAATGGGCCGGTAGCAAAGACTAATTTATTCGCTTCATCGa                                <  1:3670452/93‑1 (MQ=255)                                                              gAAAGTGAGGTGATATTTACGCGAGAACTACAGGGGGGCACCAGATCCAGTTAATGGGCCGGTAGCAAAGACTAATTTATTCGCTTCATCGaa                               >  2:1371068/1‑93 (MQ=255)                                                               aaaGTGAGGTGATATTTACGCGAGAACTACAGGGGGGCACCAGATCCAGTTAATGGGCCGGTAGCAAAGACTAATTTATTCGCTTCATCGaaa                              <  2:3028059/93‑1 (MQ=255)                                                                  gTGAGGTGATATTTACGCGAGAACTACAGGGGGGCACCAGATCCAGTTAATGGGCCGGTAGCAAAGACTAATTTATTCGCTTCATCGAAAGGt                           <  1:1366466/93‑1 (MQ=255)                                                                  gTGAGGTGATATTTACGCGAGAACTACAGGGGGGCACCAGATCCAGTTAATGGGCCGGTAGCAAAGACTAATTTATTCGCTTCATCGAAAGGt                           <  1:4285304/93‑1 (MQ=255)                                                                   tGAGGTGATATTTACGCGAGAACTACAGGGGGGCACCAGATCCAGTTAATGGGCCGGTAGCAAAGACTAATTTATTCGCTTCATCGAAAGGtt                          <  2:1363588/93‑1 (MQ=255)                                                                    gAGGTGATATTTACGCGAGAACTACAGGGGGGCACCAGATCCAGTTAATGGGCCGGTAGCAAAGACTAATTTATTCGCTTCATCGAAAGGttt                         <  1:3935224/93‑1 (MQ=255)                                                                    gAGGTGATATTTACGCGAGAACTACAGGGGGGCACCAGATCCAGTTAATGGGCCGGTAGCAAAGACTAATTTATTCGCTTCATCGAAAGGttt                         >  2:651720/1‑93 (MQ=255)                                                                    gAGGTGATATTTACGCGAGAACTACAGGGGGGCACCAGATCCAGTTAATGGGCCGGTAGCAAAGACTAATTTATTCGCTTCATCGAAAGGttt                         <  2:3333058/93‑1 (MQ=255)                                                                    gAGGTGATATTTACGCGAGAACTACA‑GGGGGCACCAGATCCAGTTAATGGGCCGGTAGCAAAGACTAATTTATTCGCTTCATCGAAAGGTTTc                        <  2:1931591/93‑1 (MQ=255)                                                                       gTGATATTTACGCGAGAACTACAGGGGGGCACCAGATCCAGTTAATGGGCCGGTAGCAAAGACTAATTTATTCGCTTCATCGAAAGGTTTCGt                      <  1:4319379/93‑1 (MQ=255)                                                                        tGATATTTACGCGAGAACTACAGGGGGGCACCAGATCCAGTTAATGGGCCGGTAGCAAAGACTAATTTATTCGCTTCATCGAAAGGTTTCGTg                     <  2:2022709/93‑1 (MQ=255)                                                                        tGATATTTACGCGAGAACTACAGGGGGGCACCAGATCCAGTTAATGGGCCGGTAGCAAAGACTAATTTATTCGCTTCATCGAAAGGTTTCGTg                     <  1:4630320/93‑1 (MQ=255)                                                                          atatTTACGCGAGAACTACAGGGGGGCACCAGATCCAGTTAATGGGCCGGTAGCAAAGACTAATTTATTCGCTTCATCGAAAGGTTTCGTGcc                   <  1:3895280/93‑1 (MQ=255)                                                                          atatTTACGCGAGAACTACAGGGGGGCACCAGATCCAGTTAATGGGCCGGTAGCAAAGACTAATTTATTCGCTTCATCGAAAGGTTTCGTGcc                   >  1:4187337/1‑93 (MQ=255)                                                                          atatTTACGCGAGAACTACAGGGGGGCACCAGATCCAGTTAATGGGCCGGTAGCAAAGACTAATTTATTCGCTTCATCGAAAGGTTTCGTGcc                   >  2:4329013/1‑93 (MQ=255)                                                                          atatTTACGCGAGAACTACAGGGGGGCACCAGATCCAGTTAATGGGCCGGTAGCAAAGACTAATTTATTCGCTTCATCGAAAGGTTTCGTGcc                   <  2:1104534/93‑1 (MQ=255)                                                                           tatTTACGCGAGAACTACAGGGGGGCACCAGATCCAGTTAATGGGCCGGTAGCAAAGACTAATTTATTCGCTTCATCGGAAGGGTTCGTGCCt                  >  2:3625826/1‑93 (MQ=255)                                                                           tatTTACGCGAGAACTACA‑GGGGGCACCAGATCCAGTTAATGGGCCGGTAGCAAAGACTAATTTATTCGCTTCATCGAAAGGTTTCGTGCCTg                 >  2:1724149/1‑93 (MQ=255)                                                                             tttACGCGAGAACTACAGGGGGGCACCAGATCCAGTTAATGGGCCGGTAGCAAAGACTAATTTATTCGCTTCATCGAAAGGTTTCGTGCCTgg                >  2:851919/1‑93 (MQ=255)                                                                              ttACGCGAGAACTACAGGGGGGCACCAGATCCAGTTAATGGGCCGGTAGCAAAGACTAATTTATTCGCTTCATCGAAAGGTTTCGTGCCTGGc               <  2:3471649/93‑1 (MQ=255)                                                                              ttACGCGAGAACTACAGGGGGGCACCAGATCCAGTTAATGGGCCGGTAGCAAAGACTAATTTATTCGCTTCATCGAAAGGTTTCGTGCCTGGc               <  1:1759012/93‑1 (MQ=255)                                                                              ttACGCGAGAACTACAGGGGGGCACCAGATCCAGTTAATGGGCCGGTAGCAAAGACTAATTTATTCGCTTCATCGAAAGGTTTCGTGCCTGGc               <  1:1728779/93‑1 (MQ=255)                                                                              ttACGCGAGAACTACAGGGGGGCACCAGATCCAGTTAATGGGCCGGTAGCAAAGACTAATTTATTCGCTTCATCGAAAGGTTTCGTGCCTGGc               <  2:2991549/93‑1 (MQ=255)                                                                               tACGCGAGAACTACAGGGGGGCACCAGATCCAGTTAATGGGCCGGTAGCAAAGACTAATTTATTCGCTTCATCGAAAGGTTTCGTGCCTGGCg              >  1:2144658/1‑93 (MQ=255)                                                                                 cgcgAGAACTACAGGGGGGCACCAGATCCAGTTAATGGGCCGGTAGCAAAGACTAATTTATTCGCTTCATCGAAAGGTTTCGTGCCTGGCGGt            <  1:3068584/93‑1 (MQ=255)                                                                                   cgAGAACTACAGGGGGGCACCAGATCCAGTTAATGGGCCGGTAGCAAAGACTAATTTATTCGCTTCATCGAAAGGTTTCGTGCCTGGCGGTAc          >  2:3804128/1‑93 (MQ=255)                                                                                     agaACTACAGGGGGGCACCAGATCCAGTTAATGGGCCGGTAGCAAAGACTAATTTATTCGCTTCATCGAAAGGTTTCGTGCCTGGCGGTACtt        >  2:3524352/1‑93 (MQ=255)                                                                                     agaACTACAGGGGGGCACCAGATCCAGTTAATGGGCCGGTAGCAAAGACTAATTTATTCGCTTCATCGAAAGGTTTCGTGCCTGGCGGTACtt        <  1:1899442/93‑1 (MQ=255)                                                                                      gaACTACAGGGGGGCACCAGATCCAGTTAATGGGCCGGTAGCAAAGACTAATTTATTCGCTTCATCGAAAGGTTTCGTGCCTGGCGGTACTTc       >  1:3652865/1‑93 (MQ=255)                                                                                      gaACTACAGGGGGGCACCAGATCCAGTTAATGGGCCGGTAGCAAAGACTAATTTATTCGCTTCATCGAAAGGTTTCGTGCCTGGCGGTACTTc       >  1:3723621/1‑93 (MQ=255)                                                                                      gaACTACAGGGGGGCACCAGATCCAGTTAATGGGCCGGTAGCAAAGACTAATTTATTCGCTTCATCGAAAGGTTTCGTGCCTGGCGGTACTTc       <  1:3801535/93‑1 (MQ=255)                                                                                        aCTACAGGGGGGCACCAGATCCAGTTAATGGGCCGGTAGCAAAGACTAATTTATTCGCTTCATCGAAAGGTTTCGTGCCTGGCGGTACTTCGt     <  1:737810/93‑1 (MQ=255)                                                                                          tACA‑GGGGGCACCAGATCCAGTTAATGGGCCGGTAGCAAAGACTAATTTATTCGCTTCATCGAAAGGTTTCGTGCCTGGCGGTACTTCGTcat  <  1:2461227/93‑1 (MQ=255)                                                                                              |                                                                                          TTGCGCTGCAAAAAAGCCACCCATATGGGCATCGACGACTAAATTTCCTTTGGTAAAAGTAGAAAGTGAGGTGATATTTACGCGAGAACTACA‑GGGGGCACCAGATCCAGTTAATGGGCCGGTAGCAAAGACTAATTTATTCGCTTCATCGAAAGGTTTCGTGCCTGGCGGTACTTCGTCAT  >  NC_000913/1751549‑1751730

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑