Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,376,227 +G coding (1053/1671 nt) menD ← 2‑oxoglutarate decarboxylase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,376,2251.G99.3% 446.9 / ‑4.3 144coding (1055/1671 nt)menD2‑oxoglutarate decarboxylase
Reads supporting (aligned to +/- strand):  ref base . (0/1);  new base G (67/76);  total (67/77)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 161 total aligned reads displayed.

GTTCAGGCAGATAGTCGCAGATGCGATGCGCCAGTTGCGCTTCGCCAAACGCATCACGGCGGGCAATAACCGCCTGCATTGCCTGTTCCGCCA‑G‑G‑CGCGGGATTTCAACGCACCAGGGCTGGCGTTTTTCTGCCGGATGCAGCTCCAGCCAGTCGGCAATATTGGCAATTAAGCGACGT  >  NC_000913/2376133‑2376311
                                                                                             |                                                                                        
gTTCAGGCAGATAGTCGCAGATGCGATGCGCCAGTTGCGCTTCGCCAAACGCATCACGGCGGGCAATAACCGCCTGCATTGCCTGTTCCGCCa                                                                                           <  2:830333/93‑1 (MQ=255)
 ttCAGGCAGATAGTCGCAGATGCGATGCGCCAGTTGCGCTTCGCCAAACGCATCACGGCGGGCAATAACCGCCTGCATTGTCTGTTCCGCCA‑g                                                                                         <  2:1341724/93‑1 (MQ=255)
  tCAGGCAGATAGTCGCAGATGCGATGCGCCAGTTGCGCTTCGCCAAACGCATCACGGCGGGCAATAACCGCCTGCATTGCCTGTTCCGCCA‑g‑g                                                                                       <  2:3716195/93‑1 (MQ=255)
  tCAGGCAGATAGTCGCAGATGCGATGCGCCAGTTGCGCTTCGCCAAACGCATCACGGCGGGCAATAACCGCCTGCATTGCCTGTTCCGCCA‑g‑g                                                                                       <  1:3791318/93‑1 (MQ=255)
    aGGCAGATAGTCGCAGATGCGATGCGCCAGTTGCGCTTCGCCAAACGCATCACGGCGGGCAATAACCGCCTGCATTGCCTGTTCCGCCA‑g‑ggc                                                                                     >  2:4085197/1‑91 (MQ=255)
     ggCAGATAGTCGCAGATGCGATGCGCCAGTTGCGCTTCGCCAAACGCATCACGGCGGGCAATAACCGCCTGCATTGCCTGTTCCGCCA‑g‑ggcg                                                                                    >  1:1136389/1‑90 (MQ=255)
     ggCAGATAGTCGCAGATGCGATGCGCCAGTTGCGCTTCGCCAAACGCATCACGGCGGGCAATAACCGCCTGCATTGCCTGTTCCGCCA‑g‑ggcg                                                                                    >  2:1357499/1‑90 (MQ=255)
     ggCAGATAGTCGCAGATGCGATGCGCCAGTTGCGCTTCGCCAAACGCATCACGGCGGGCAATAACCGCCTGCATTGCCTGTTCCGCCA‑g‑ggcg                                                                                    >  1:1753348/1‑90 (MQ=255)
      gCAGATAGTCGCAGATGCGATGCGCCAGTTGCGCTTCGCCAAACGCATCACGGCGGGCAATAACCGCCTGCATTGCCTGTTCCGCCA‑g‑ggcgc                                                                                   <  1:3323210/93‑5 (MQ=255)
        aGATAGTCGCAGATGCGATGCGCCAGTTGCGCTTCGCCAAACGCATCACGGCGGGCAATAACCGCCTGCATTGCCTGTTCCGCCAGG‑G‑CGCgg                                                                                 <  2:4585618/93‑1 (MQ=255)
        aGATAGTCGCAGATGCGATGCGCCAGTTGCGCTTCGCCAAACGCATCACGGCGGGCAATAACCGCCTGCATTGCCTGTTCCGCCAGG‑G‑CGCgg                                                                                 >  1:458862/1‑93 (MQ=255)
          aTAGTCGCAGATGCGATGCGCCAGTTGCGCTTCGCCAAACGCATCACGGCGGGCAATAACCGCCTGCATTGCCTGTTCCGCCAGG‑G‑CGCGGGa                                                                               >  1:2339640/1‑93 (MQ=255)
           tAGTCGCAGATGCGATGCGCCAGTTGCGCTTCGCCAAACGCATCACGGCGGGCAATAACCGCCTGCATTGCCTGTTCCGCCAGG‑G‑CGCGGGAt                                                                              <  1:3510785/93‑1 (MQ=255)
              tCGCAGATGCGATGCGCCAGTTGCGCTTCGCCAAACGCATCACGGCGGGCAATAACCGCCTGCATTGCCTGTTCCGCCAGG‑G‑CGCGGGATTTc                                                                           <  1:1381503/93‑1 (MQ=255)
              tCGCAGATGCGATGCGCCAGTTGCGCTTCGCCAAACGCATCACGGCGGGCAATAACCGCCTGCATTGCCTGTTCCGCCAGG‑G‑CGCGGGATTTc                                                                           <  2:3041566/93‑1 (MQ=255)
                gCAGATGCGATGCGCCAGTTGCGCTTCGCCAAACGCATCACGGCGGGCAATAACCGCCTGCATTGCCTGTTCCGCCAGG‑G‑CGCGGGATTTCaa                                                                         >  2:1823646/1‑93 (MQ=255)
                gCAGATGCGATGCGCCAGTTGCGCTTCGCCAAACGCATCACGGCGGGCAATAACCGCCTGCATTGCCTGTTCCGCCAGG‑G‑CGCGGGATTTCaa                                                                         <  1:3459311/93‑1 (MQ=255)
                gCAGATGCGATGCGCCAGTTGCGCTTCGCCAAACGCATCACGGCGGGCAATAACCGCCTGCATTGCCTGTTCCGCCAGG‑G‑CGCGGGATTTCaa                                                                         <  1:2560732/93‑1 (MQ=255)
                gCAGATGCGATGCGCCAGTTGCGCTTCGCCAAACGCATCACGGCGGGCAATAACCGCCTGCATTGCCTGTTCCGCCAGG‑G‑CGCGGGATTTCaa                                                                         <  2:1914352/93‑1 (MQ=255)
                gCAGATGCGATGCGCCAGTTGCGCTTCGCCAAACGCATCACGGCGGGCAATAACCGCCTGCATTGCCTGTTCCGCCAGG‑G‑CGCGGGATTTCaa                                                                         <  2:2027227/93‑1 (MQ=255)
                 cAGATGCGATGCGCCAGTTGCGCTTCGCCAAACGCATCACGGCGGGCAATAACCGCCTGCATTGCCTGTTCCGCCAGG‑G‑CGCGGGATTTCAAc                                                                        >  1:3245316/1‑93 (MQ=255)
                 cAGATGCGATGCGCCAGTTGCGCTTCGCCAAACGCATCACGGCGGGCAATAACCGCCTGCATTGCCTGTTCCGCCAGG‑G‑CGCGGGATTTCAAc                                                                        >  1:2746493/1‑93 (MQ=255)
                 cAGATGCGATGCGCCAGTTGCGCTTCGCCAAACGCATCACGGCGGGCAATAACCGCCTGCATTGCCTGTTCCGCCAGG‑G‑CGCGGGATTTCAAc                                                                        >  1:2571470/1‑93 (MQ=255)
                 cAGATGCGATGCGCCAGTTGCGCTTCGCCAAACGCATCACGGCGGGCAATAACCGCCTGCATTGCCTGTTCCGCCAGG‑G‑CGCGGGATTTCAAc                                                                        <  2:3832534/93‑1 (MQ=255)
                   gatgcgatgcGCCAGTTGCGCTTCGCCAAACGCATCACGGCGGGCAATAACCGCCTGCATTGCCTGTTCCGCCAGG‑G‑CGCGGGATTTCAACGc                                                                      >  1:3193995/1‑93 (MQ=255)
                   gatgcgatgcGCCAGTTGCGCTTCGCCAAACGCATCACGGCGGGCAATAACCGCCTGCATTGCCTGTTCCGCCAGG‑G‑CGCGGGATTTCAACGc                                                                      >  1:4108461/1‑93 (MQ=255)
                        gatgcgCCAGTTGCGCTTCGCCAAACGCATCACGGCGGGCAATAACCGCCTGCATTGCCTGTTCCGCCAGG‑G‑CGCGGGATTTCAACGCACCAg                                                                 >  1:433234/1‑93 (MQ=255)
                          tgcgCCAGTTGCGCTTCGCCAAACGCATCACGGCGGGCAATAACCGCCTGCATTGCCTGTTCCGCCAGG‑G‑CGCGGGATTTCAACGCACCAggg                                                               >  1:227515/1‑93 (MQ=255)
                           gccccAGTTGCGCTTCGCCAAACGCATCACGGCGGGCAATAACCGCCTGCATTGCCTGTTCCGCCAGG‑G‑CGCGGGATTTCAACGCACCAGGGc                                                              <  2:4126271/90‑1 (MQ=255)
                            cgcCAGTTGCGCTTCGCCAAACGCATCACGGCGGGCAATAACCGCCTGCATTGCCTGTTCCGCCAGG‑G‑CGCGGGATTTCAACGCACCAGGGCt                                                             <  1:3895159/93‑1 (MQ=255)
                             gcCAGTTGCGCTTCGCCAAACGCATCACGGCGGGCAATAACCGCCTGCATTGCCTGTTCCGCCAGG‑G‑CGCGGGATTTCAACGCACCAGGGCTg                                                            <  2:2441658/93‑1 (MQ=255)
                              cgaGTTGCGCTTCGCTAAACGCATCACGGCGGGAAATAACCGCCTGCATTGCCTGTTCCGTCA‑GTG‑CGCGGTATTTCAACGCACCAGGGCTgg                                                           <  2:3491738/91‑1 (MQ=255)
                              ccAGTTGCGCTTCGCCAAACGCATCACGGCGGGCAATAACCGCCTGCATTGCCTGTTCCGCCAGG‑G‑CGCGGGATTTCAACGCACCAGGGCTgg                                                           >  1:4599351/1‑93 (MQ=255)
                              ccAGTTGCGCTTCGCCAAACGCATCACGGCGGGCAATAACCGCCTGCATTGCCTGTTCCGCCAGG‑G‑CGCGGGATTTCAACGCACCAGGGCTgg                                                           <  1:302651/93‑1 (MQ=255)
                               cAGTTGCGCTTCGCCAAACGCATCACGGCGGGCAATAACCGCCTGCATTGCCTGTTCCGCCAGG‑G‑CGCGGGATTTCAACGCACCAGGGCTGGc                                                          >  2:4309491/1‑93 (MQ=255)
                                 gTTGCGCTTCGCCAAACGCATCACGGCGGGCAATAACCGCCTGCATTGCCTGTTCCGCCAGG‑G‑CGCGGGATTTCAACGCACCAGGGCTGGCGt                                                        >  2:1538621/1‑93 (MQ=255)
                                 gTTGCGCTTCGCCAAACGCATCACGGCGGGCAATAACCGCCTGCATTGCCTGTTCCGCCAGG‑G‑CGCGGGATTTCAACGCACCAGGGCTGGCGt                                                        >  1:4517419/1‑93 (MQ=255)
                                  ttGCGCTTCGCCAAACGCATCACGGCGGGCAATAACCGCCTGCATTGCCTGTTCCGCCAGG‑G‑CGCGGGATTTCAACGCACCAGGGCTGGCGtt                                                       <  1:4639999/93‑1 (MQ=255)
                                  ttGCGCTTCGCCAAACGCATCACGGCGGGCAATAACCGCCTGCATTGCCTGTTCCGCCAGG‑G‑CGCGGGATTTCAACGCACCAGGGCTGGCGtt                                                       >  1:2990986/1‑93 (MQ=255)
                                    gcgcTTCGCCAAACGCATCACGGCGGGCAATAACCGCCTGCATTGCCTGTTCCGCCAGG‑G‑CGCGGGATTTCAACGCACCAGGGCTGGCGtttt                                                     >  1:1012357/1‑93 (MQ=255)
                                      gcTTCGCCAAACGCATCACGGCGGGCAATAACCGCCTGCATTGCCTGTTCCGCCAGG‑G‑CGCGGGATTTCAACGCACCAGGGCTGGCGTTTTTc                                                   <  2:4159119/93‑1 (MQ=255)
                                      gcTTCGCCAAACGCATCACGGCGGGCAATAACCGCCTGCATTGCCTGTTCCGCCAGG‑G‑CGCGGGATTTCAACGCACCAGGGCTGGCGTTTTTc                                                   <  1:946334/93‑1 (MQ=255)
                                      gcTTCGCCAAACGCATCACGGCGGGAAATAACCGCCTGCATTGCCTGTTCCGCCA‑G‑GCCGCGGGATTTCAACGCACCAGGGCTGGCGTTTTTc                                                   >  2:3364686/1‑93 (MQ=255)
                                         tCGCCAAACGCATCACGGCGGGCAATAACCGCCTGCATTGCCTGTTCCGCCAGG‑G‑CGCGGGATTTCAACGCACCAGGGCTGGCGTTTTTCTGc                                                >  1:3571089/1‑93 (MQ=255)
                                         tCGCCAAACGCATCACGGCGGGCAATAACCGCCTGCATTGCCTGTTCCGCCAGG‑G‑CGCGGGATTTCAACGCACCAGGGCTGGCGTTTTTCTGc                                                <  2:787421/93‑1 (MQ=255)
                                           gCCAAACGCATCACGGCGGGCAATAACCGCCTGCATTGCCTGTTCCGCCAGG‑G‑CGCGGGATTTCAACGCACCAGGGCTGGCGTTTTTCTGCCg                                              >  1:3077297/1‑93 (MQ=255)
                                             cAAACGCATCACGGCGGGCAATAACCGCCTGCATTGCCTGTTCCGCCAGG‑G‑CGCGGGATTTCAACGCACCAGGGCTGGCGTTTTTCTGCCGGa                                            <  2:2351209/93‑1 (MQ=255)
                                             cAAACGCATCACGGCGGGCAATAACCGCCTGCATTGCCTGTTCCGCCAGG‑G‑CGCGGGATTTCAACGCACCAGGGCTGGCGTTTTTCTGCCGGa                                            <  1:751767/93‑1 (MQ=255)
                                              aaaCGCATCACGGCGGGCAATAACCGCCTGCATTGCCTGTTCCGCCAGG‑G‑CGCGGGATTTCAACGCGCCAGGGCTGGCGTTTTTCTGCCGGAt                                           <  1:1275113/93‑1 (MQ=255)
                                              aaaCGCATCACGGCGGGCAATAACCGCCTGCATTGCCTGTTCCGCCAGG‑G‑CGCGGGATTTCAACGCACCAGGGCTGGCGTTTTTCTGCCGGAt                                           <  1:4117032/93‑1 (MQ=255)
                                               aaCGCATCACGGCGGGCAATAACCGCCTGCATTGCCTGTTCCGCCAGG‑G‑CGCGGGATTTCAACGCACCAGGGCTGGCGTTTTTCTGCCGGATg                                          <  2:4203460/93‑1 (MQ=255)
                                                 cGCATCACGGCGGGCAATAACCGCCTGCATTGCCTGTTCCGCCAGG‑G‑CGCGGGATTTCAACGCACCAGGGCTGGCGTTTTTCTGCCGGATGCa                                        <  2:1073229/93‑1 (MQ=255)
                                                   cATCACGGCGGGCAATAACCGCCTGCATTGCCTGTTCCGCCAGG‑G‑CGCGGGATTTCAACGCACCAGGGCTGGCGTTTTTCTGCCGGATGCAGc                                      <  1:1795200/93‑1 (MQ=255)
                                                   cATCACGGCGGGCAATAACCGCCTGCATTGCCTGTTCCGCCAGG‑G‑CGCGGGATTTCAACGCACCAGGGCTGGCGTTTTTCTGCCGGATGCAGc                                      <  1:3739888/93‑1 (MQ=255)
                                                   cATCACGGCGGGCAATAACCGCCTGCATTGCCTGTTCCGCCAGG‑G‑CGCGGGATTTCAACGCACCAGGGCTGGCGTTTTTCTGCCGGATGCAGc                                      <  1:3089771/93‑1 (MQ=255)
                                                   cATCACGGCGGGCAATAACCGCCTGCATTGCCTGTTCCGCCAGG‑G‑CGCGGGATTTCAACGCACCAGGGCTGGCGTTTTTCTGCCGGATGCAGc                                      >  1:4090317/1‑93 (MQ=255)
                                                   cATCACGGCGGGCAATAACCGCCTGCATTGCCTGTTCCGCCAGG‑G‑CGCGGGATTTCAACGCACCAGGGCTGGCGTTTTTCTGCCGGATGCAGc                                      <  1:3810953/93‑1 (MQ=255)
                                                      cACGGCGGGCAATAACCGCCTGCATTGCCTGTTCCGCCAGG‑G‑CGCGGGATTTCAACGCACCAGGGCTGGCGTTTTTCTGCCGGATGCAGCTcc                                   <  1:2718654/93‑1 (MQ=255)
                                                      cACGGCGGGCAATAACCGCCTGCATTGCCTGTTCCGCCAGG‑G‑CGCGGGATTTCAACGCACCAGGGCTGGCGTTTTTCTGCCGGATGCAGCTcc                                   >  2:4610284/1‑93 (MQ=255)
                                                      cACGGCGGGCAATAACCGCCTGCATTGCCTGTTCCGCCAGG‑G‑CGCGGGATTTCAACGCACCAGGGCTGGCGTTTTTCTGCCGGATGCAGCTcc                                   <  2:2169468/93‑1 (MQ=255)
                                                      cACGGCGGGCAATAACCGCCTGCATTGCCTGTTCCGCCAGG‑G‑CGCGGGATTTCAACGCACCAGGGCTGGCGTTTTTCTGCCGGATGCAGCTcc                                   <  1:1723318/93‑1 (MQ=255)
                                                       aCGGCGGGCAATAACCGCCTGCATTGCCTGTTCCGCCAGG‑G‑CGCGGGATTTCAACGCACCAGGGCTGGCGTTTTTCTGCCGGATGCAGCTcca                                  <  1:3437402/93‑1 (MQ=255)
                                                       aCGGCGGGCAATAACCGCCTGCATTGCCTGTTCCGCCAGG‑G‑CGCGGGATTTCAACGCACCAGGGCTGGCGTTTTTCTGCCGGATGCAGCTcca                                  >  2:2120918/1‑93 (MQ=255)
                                                         ggcggGCAATAACCGCCTGCATTGCCTGTTCCGCCAGG‑G‑CGCGGGATTTCAACGCACCAGGGCTGGCGTTTTTCTGCCGGATGCAGCTccagc                                <  1:847424/93‑1 (MQ=255)
                                                            gggCAATAACCGCCTGCATTGCCTGTTCCGCCAGG‑G‑CGCGGGATTTCAACGCACCAGGGCTGGCGTTTTTCTGCCGGATGCAGCTccagccag                             >  2:1634439/1‑93 (MQ=255)
                                                            gggCAATAACCGCCTGCATTGCCTGTTCCGCCAGG‑G‑CGCGGGATTTCAACGCACCAGGGCTGGCGTTTTTCTGCCGGATGCAGCTccagccag                             <  2:2804285/93‑1 (MQ=255)
                                                              gCAATAACCGCCTGCATTGCCTGTTCCGCCAGG‑G‑CGCGGGATTTCAACGCACCAGGGCTGGCGTTTTTCTGCCGGATGCAGCTCCAGCCAGTc                           >  2:4570822/1‑93 (MQ=255)
                                                                aaTAACCGCCTGCATTGCCTGTTCCGCCAGG‑G‑CGCGGGATTTCAACGCACCAGGGCTGGCGTTTTTCTGCCGGATGCAGCTCCAGCCAGTCgg                         <  1:2068782/93‑1 (MQ=255)
                                                                    aCCGCCTGCATTGCCTGTTCCGCCAGG‑G‑CGCGGGATTTCAACGCACCAGGGCTGGCGTTTTTCTGCCGGATGCAGCTCCAGCCAGTCGGCAat                     >  1:945473/1‑93 (MQ=255)
                                                                    aCCGCCTGCATTGCCTGTTCCGCCAGG‑G‑CGCGGGATTTCAACGCACCAGGGCTGGCGTTTTTCTGCCGGATGCAGCTCCAGCCAGTCGGCAat                     >  1:4381457/1‑93 (MQ=255)
                                                                      cGCCTGCATTGCCTGTTCCGCCAGG‑G‑CGCGGGATTTCAACGCACCAGGGCTGGCGTTTTTCTGCCGGATGCAGCTCCAGCCAGTCGGCAatat                   <  2:1837885/93‑1 (MQ=255)
                                                                      cGCCTGCATTGCCTGTTCCGCCAGG‑G‑CGCGGGATTTCAACGCACCAGGGCTGGCGTTTTTCTGCCGGATGCAGCTCCAGCCAGTCGGCAatat                   <  2:361642/93‑1 (MQ=255)
                                                                      cGCCTGCATTGCCTGTTCCGCCAGG‑G‑CGCGGGATTTCAACGCACCAGGGCTGGCGTTTTTCTGCCGGATGCAGCTCCAGCCAGTCGGCAatat                   <  1:4244162/93‑1 (MQ=255)
                                                                       gCCTGCATTGCCTGTTCCGCCAGG‑G‑CGCGGGATTTCAACGCACCAGGGCTGGCGTTTTTCTGCCGGATGCAGCTCCAGCCAGTCGGCAATAtt                  <  1:3920439/93‑1 (MQ=255)
                                                                       gCCTGCATTGCCTGTTCCGCCAGG‑G‑CGCGGGATTTCAACGCACCAGGGCTGGCGTTTTTCTGCCGGATGCAGCTCCAGCCAGTCGGCAATAtt                  <  1:1934306/93‑1 (MQ=255)
                                                                       gCCTGCATTGCCTGTTCCGCCAGG‑G‑CGCGGGATTTCAACGCACCAGGGCTGGCGTTTTTCTGCCGGATGCAGCTCCAGCCAGTCGGCAATAtt                  >  2:253987/1‑93 (MQ=255)
                                                                        ccTGCATTGCCTGTTCCGCCAGG‑G‑CGCGGGATTTCAACGCACCAGGGCTGGCGTTTTTCTGCCGGATGCAGCTCCAGCCAGTCGGCAATATTg                 <  1:4534531/93‑1 (MQ=255)
                                                                        ccTGCATTGCCTGTTCCGCCAGG‑G‑CGCGGGATTTCAACGCACCAGGGCTGGCGTTTTTCTGCCGGATGCAGCTCCAGCCAGTCGGCAATATTg                 <  2:1617198/93‑1 (MQ=255)
                                                                         cTGCATTGCCTGTTCCGCCAGG‑G‑CGCGGGATTTCAACGCACCAGGGCTGGCGTTTTTCTGCCGGATGCAGCTCCAGCCAGTCGGCAATATTgg                <  2:2028826/93‑1 (MQ=255)
                                                                          tGCATTGCCTGTTCCGCCAGG‑G‑CGCGGGATTTCAACGCACCAGGGCTGGCGTTTTTCTGCCGGATGCAGCTCCAGCCAGTCGGCAATATTGGc               >  1:1332605/1‑93 (MQ=255)
                                                                           gCATTGCCTGTTCCGCCAGG‑G‑CGCGGGATTTCAACGCACCAGGGCTGGCGTTTTTCTGCCGGATGCAGCTCCAGCCAGTCGGCAATATTGGCa              >  2:2714295/1‑93 (MQ=255)
                                                                           gCATTGCCTGTTCCGCCAGG‑G‑CGCGGGATTTCAACGCACCAGGGCTGGCGTTTTTCTGCCGGATGCAGCTCCAGCCAGTCGGCAATATTGGCa              <  1:3576529/93‑1 (MQ=255)
                                                                            cATTGCCTGTTCCGCCAGG‑G‑CGCGGGATTTCAACGCACCAGGGCTGGCGTTTTTCTGCCGGATGCAGCTCCAGCCAGTCGGCAATATTGGCaa             >  1:3364790/1‑93 (MQ=255)
                                                                             aTTGCCTGTTCCGCCAGG‑G‑CGCGGGATTTCAACGCACCAGGGCTGGCGTTTTTCTGCCGGATGCAGCTCCAGCCAGTCGGCAATATTAGCAAt            >  1:2691568/1‑93 (MQ=255)
                                                                              ttGCCTGTTCCGCCAGG‑G‑CGCGGGATTTCAACGCACCAGGGCTGGCGTTTTTCTGCCGGATGCAGCTCCAGCCAGTCGGCAATATTGGCAAtt           >  1:4160179/1‑93 (MQ=255)
                                                                              ttGCCTGTTCCGCCAGG‑G‑CGCGGGATTTCAACGCACCAGGGCTGGCGTTTTTCTGCCGGATGCAGCTCCAGCCAGTCGGCAATATTGGCAAtt           >  1:1829496/1‑93 (MQ=255)
                                                                               tGCCTGTTCCGCCAGG‑G‑CGCGGGATTTCAACGCACCAGGGCTGGCGTTTTTCTGCCGGATGCAGCTCCAGCCAGTCGGCAATATTGGCAATTa          <  2:4328880/93‑1 (MQ=255)
                                                                               tGCCTGTTCCGCCAGG‑G‑CGCGGGATTTCAACGCACCAGGGCTGGCGTTTTTCTGCCGGATGCAGCTCCAGCCAGTCGGCAATATTGGCAATTa          <  2:2442843/93‑1 (MQ=255)
                                                                                gCCTGTTCCGCCAGG‑G‑CGCGGGATTTCAACGCACCAGGGCTGGCGTTTTTCTGCCGGATGCAGCTCCAGCCAGTCGGCAATATTGGCAATTaa         >  1:2871645/1‑93 (MQ=255)
                                                                                 ccTGTTCCGCCAGG‑G‑CGCGGGATTTCAACGCACCAGGGCTGGCGTTTTTCTGCCGGATGCAGCTCCAGCCAGTCGGCAATATTGGCAATTAAg        >  2:3306908/1‑93 (MQ=255)
                                                                                  cTGTTCCGCCAGG‑G‑CGCGGGATTTCAACGCACCAGGGCTGGCGTTTTTCTGCCGGATGCAGCTCCAGCCAGTCGGCAATATTGGCAATTAAGc       >  1:202229/1‑93 (MQ=255)
                                                                                  cTGTTCCGCCAGG‑G‑CGCGGGATTTCAACGCACCAGGGCTGGCGTTTTTCTGCCGGATGCAGCTCCAGCCAGTCGGCAATATTGGCAATTAAGc       <  1:3238431/93‑1 (MQ=255)
                                                                                  cTGTTCCGCCAGG‑G‑CGCGGGATTTCAACGCACCAGGGCTGGCGTTTTTCTGCCGGATGCAGCTCCAGCCAGTCGGCAATATTGGCAATTAAGc       <  1:1949912/93‑1 (MQ=255)
                                                                                  cTGTTCCGCCAGG‑G‑CGCGGGATTTCAACGCACCAGGGCTGGCGTTTTTCTGCCGGATGCAGCTCCAGCCAGTCGGCAATATTGGCAATTAAGc       >  2:4427041/1‑93 (MQ=255)
                                                                                   tGTTCCGCCAGG‑G‑CGCGGGATTTCAACGCACCAGGGCTGGCGTTTTTCTGCCGGATGCAGCTCCAGCCAGTCGGCAATATTGGCAATTAAGCg      >  1:2492939/1‑93 (MQ=255)
                                                                                    gTTCCGCCAGG‑G‑CGCGGGATTTCAACGCACCAGGGCTGGCGTTTTTCTGCCGGATGCAGCTCCAGCCAGTCGGCAATATTGGCAATTAAGCGa     <  2:4564919/93‑1 (MQ=255)
                                                                                    gTTCCGCCAGG‑G‑CGCGGGATTTCAACGCACCAGGGCTGGCGTTTTTCTGCCGGATGCAGCTCCAGCCAGTCGGCAATATTGGCAATTAAGCGa     <  1:4450346/93‑1 (MQ=255)
                                                                                     ttCCGCCAGG‑G‑CGCGGGATTTCAACGCACCAGGGCTGGCGTTTTTCTGCCGGATGCAGCTCCAGCCAGTCGGCAATATTGGCAATTAAGCGAc    <  2:2844662/93‑1 (MQ=255)
                                                                                      tCCGCCAGG‑G‑CGCGGGATTTCAACGCACCAGGGCTGGCGTTTTTCTGCCGGATGCAGCTCCAGCCAGTCGGCAATATTGGCAATTAAGCGACg   <  1:4216047/93‑1 (MQ=255)
                                                                                       ccGCCAGG‑G‑CGCGGGATTTCAACGCACCAGGGCTGGCGTTTTTCTGCCGGATGCAGCTCCAGCCAGTCGGCAATATTGGCAATTAAGCGACGt  <  1:1595973/93‑1 (MQ=255)
                                                                                             |                                                                                        
GTTCAGGCAGATAGTCGCAGATGCGATGCGCCAGTTGCGCTTCGCCAAACGCATCACGGCGGGCAATAACCGCCTGCATTGCCTGTTCCGCCA‑G‑G‑CGCGGGATTTCAACGCACCAGGGCTGGCGTTTTTCTGCCGGATGCAGCTCCAGCCAGTCGGCAATATTGGCAATTAAGCGACGT  >  NC_000913/2376133‑2376311

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑