BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,501,888	+C	coding (618/2496 nt)	b2383 ←	putative PTS system enzyme IIA component, enzyme I

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,501,884	1	.	C	100.0%	453.4 / NA	146	L208V (CTG→GTG)	b2383	putative PTS system enzyme IIA component, enzyme I
Reads supporting (aligned to +/- strand): ref base . (0/0); new base C (86/60); total (86/60)

Only 100 of 167 total aligned reads displayed.

AATGTCGCTAACACGTTCGCGCAGATAATCGCTGGCAGAGGCAGAAAGTTTGGCGCAAACCTGCTCCATATTGCTGATGATCGCCGCCCCCAG‑CCCCTGATGCTGTTCTGTCATCAGGCGACGGATATTGCCTGCAAATTCATCATCCTGAATCAGCGACAAATGGGCGCTGAGGATAGT  >  NC_000913/2501792‑2501971

aaTGTCGCTAACACGTTCGCGCAGATAATCGCTGACAGAGGCAGAAAGTTTGGCGCAAACCTGCTCCATATTGCTGATGATCGCCGCCCCCAg                                                                                          >  2:4629940/1‑93 (MQ=255)

  tGTCGCTAACACGTTCGCGCAGATAATCGCTGGCAGAGGCAGAAAGTTTGGCGCAAACCTGCTCCATATTGCTGATGATCGCCGCCCCCAG‑cc                                                                                       >  2:3775071/1‑93 (MQ=255)

   gTCGCTAACACGTTCGCGCAGATAATCGCTGGCAGAGGCAGAAAGTTTGGCGCAAACCTGCTCCATATTGCTGATGATCGCCGCCCCCAG‑ccc                                                                                      >  2:2906294/1‑93 (MQ=255)

    tCGCTAACACGTTCGCGCAGATAATCGCTGGCAGAGGCAGAAAGTTTGGCGCAAACCTGCTCCATATTGCTGATGATCGCCGCCCCCAG‑cccc                                                                                     <  1:3179365/93‑1 (MQ=255)

     cGCTAACACGTTCGCGCAGATAATCGCTGGCAGAGGCAGAAAGTTTGGCGCAAACCTGCTCCATATTGCTGATGATCGCCGCCCCCAG‑ccccc                                                                                    <  1:4148433/93‑2 (MQ=255)

     cGCTAACACGTTCGCGCAGATAATCGCTGGCAGAGGCAGAAAGTTTGGCGCAAACCTGCTCCATATTGCTGATGATCGCCGCCCCCAG‑ccccc                                                                                    >  1:4576858/1‑92 (MQ=255)

      gCTAACACGTTCGCGCAGATAATCGCTGGCAGAGGCAGAAAGTTTGGCGCAAACCTGCTCCATATTGCTGATGATCGCCGCCCCCAG‑ccccct                                                                                   >  2:2814713/1‑91 (MQ=255)

       cTAACACGTTCGCGCAGATAATCGCTGGCAGAGGCAGAAAGTTTGGCGCAAACCTGCTCCATATTGCTGATGATCGCCGCCCCCAG‑ccccctg                                                                                  >  1:3749110/1‑90 (MQ=255)

       cTAACACGTTCGCGCAGATAATCGCTGGCAGAGGCAGAAAGTTTGGCGCAAACCTGCTCCATATTGCTGATGATCGCCGCCCCCAG‑ccccctg                                                                                  >  1:3751953/1‑90 (MQ=255)

        tAACACGTTCGCGCAGATAATCGCTGGCAGAGGCAGAAAGTTTGGCGCAAACCTGCTCCATATTGCTGATGATCGCCGCCCCCAG‑ccccctga                                                                                 >  2:3443897/1‑89 (MQ=255)

        tAACACGTTCGCGCAGATAATCGCTGGCAGAGGCAGAAAGTTTGGCGCAAACCTGCTCCATATTGCTGATGATCGCCGCCCCCAG‑ccccctga                                                                                 <  2:348586/93‑5 (MQ=255)

        tAACACGTTCGCGCAGATAATCGCTGGCAGAGGCAGAAAGTTTGGCGCAAACCTGCTCCATATTGCTGATGATCGCCGCCCCCAG‑ccccctga                                                                                 <  1:4666418/93‑5 (MQ=255)

        tAACACGTTCGCGCAGATAATCGCTGGCAGAGGCAGAAAGTTTGGCGCAAACCTGCTCCATATTGCTGATGATCGCCGCCCCCAG‑ccccctga                                                                                 <  1:3931012/93‑5 (MQ=255)

          acacGTTCGCGCAGATAATCGCTGGCAGAGGCAGAAAGTTTGGCGCAAACCTGCTCCATATTGCTGATGATCGCCGCCCCCAGCCCCCTGATg                                                                                >  1:3208677/1‑93 (MQ=255)

            acGTTCGCGCAGATAATCGCTGGCAGAGGCAGAAAGTTTGGCGCAAACCTGCTCCATATTGCTGATGATCGCCGCCCCCAGCCCCCTGATGCt                                                                              <  2:3180682/93‑1 (MQ=255)

             cGTTCGCGCAGATAATCGCTGGCAGAGGCAGAAAGTTTGGCGCAAACCTGCTCCATATTGCTGATGATCGCCGCCCCCAGCCCCCTGATGCTg                                                                             >  1:1630823/1‑93 (MQ=255)

               ttCGCGCAGATAATCGCTGGCAGAGGCAGAAAGTTTGGCGCAAACCTGCTCCATATTGCTGATGATCGCCGCCCCCAGCCCCCTGATGCTGtt                                                                           >  2:2667973/1‑93 (MQ=255)

               ttCGCGCAGATAATCGCTGGCAGAGGCAGAAAGTTTGGCGCAAACCTGCTCCATATTGCTGATGATCGCCGCCCCCAGCCCCCTGATGCTGtt                                                                           >  1:2863140/1‑93 (MQ=255)

               ttCGCGCAGATAATCGCTGGCAGAGGCAGAAAGTTTGGCGCAAACCTGCTCCATATTGCTGATGATCGCCGCCCCCAGCCCCCTGATGCTGtt                                                                           >  1:738642/1‑93 (MQ=255)

               ttCGCGCAGATAATCGCTGGCAGAGGCAGAAAGTTTGGCGCAAACCTGCTCCATATTGCTGATGATCGCCGCCCCCAGCCCCCTGATGCTGtt                                                                           >  1:1425474/1‑93 (MQ=255)

                tCGCGCAGATAATCGCTGGCAGAGGCAGAAAGTTTGGCGCAAACCTGCTCCATATTGCTGATGATCGCCGCCCCCAGCCCCCTGATGCTGTTc                                                                          <  1:2809464/93‑1 (MQ=255)

                tCGCGCAGATAATCGCTGGCAGAGGCAGAAAGTTTGGCGCAAACCTGCTCCATATTGCTGATGATCGCCGCCCCCAGCCCCCTGATGCTGTTc                                                                          <  2:4157391/93‑1 (MQ=255)

                 cgcgCAGATAATCGCTGGCAGAGGCAGAAAGTTTGGCGCAAACCTGCTCCATATTGCTGATGATCGCCGCCCCCAGCCCCCTGATGCTGTTCt                                                                         >  2:4260345/1‑93 (MQ=255)

                  gcgcAGATAATCGCTGGCAGAGGCAGAAAGTTTGGCGCAAACCTGCTCCATATTGCTGATGATCGCCGCCCCCAGCCCCCTGATGCTGTTCTg                                                                        >  1:2466712/1‑93 (MQ=255)

                   cgcAGATAATCGCTGGCAGAGGCAGAAAGTTTGGCGCAAACCTGCTCCATATTGCTGATGATCGCCGCCCCCAGCCCCCTGATGCTGTTCTGt                                                                       <  1:1244424/93‑1 (MQ=255)

                   cgcAGATAATCGCTGGCAGAGGCAGAAAGTTTGGCGCAAACCTGCTCCATATTGCTGATGATCGCCGCCCCCAGCCCCCTGATGCTGTTCTGt                                                                       <  1:3671073/93‑1 (MQ=255)

                     cAGATAATCGCTGGCAGAGGCAGAAAGTTTGGCGCAAACCTGCTCCATATTGCTGATGATCGCCGCCCCCAGCCCCCTGATGCTGTTCTGtca                                                                     >  1:3514947/1‑93 (MQ=255)

                     cAGATAATCGCTGGCAGAGGCAGAAAGTTTGGCGCAAACCTGCTCCATATTGCTGATGATCGCCGCCCCCAGCCCCCTGATGCTGTTCTGtca                                                                     <  1:824399/93‑1 (MQ=255)

                      aGATAATCGCTGGCAGAGGCAGAAAGTTTGGCGCAAACCTGCTCCATATTGCTGATGATCGCCGCCCCCAGCCCCCTGATGCTGTTCTGtcat                                                                    <  2:2078883/93‑1 (MQ=255)

                        aTAATCGCTGGCAGAGGCAGAAAGTTTGGCGCAAACCTGCTCCATATTGCTGATGATCGCCGCCCCCAGCCCCCTGATGCTGTTCTGtcatca                                                                  <  1:1019887/93‑1 (MQ=255)

                         tAATCGCTGGCAGAGGCAGAAAGTTTGGCGCAAACCTGCTCCATATTGCTGATGATCGCCGCCCCCAGCCCCCTGATGCTGTTCTGTCATCAg                                                                 >  1:3356403/1‑93 (MQ=255)

                             cGCTGGCAGAGGCAGAAAGTTTGGCGCAAACCTGCTCCATATTGCTGATGATCGCCGCCCCCAGCCCCCTGATGCTGTTCTGTCATCAGGCGa                                                             >  1:119380/1‑93 (MQ=255)

                             cGCTGGCAGAGGCAGAAAGTTTGGCGCAAACCTGCTCCATATTGCTGATGATCGCCGCCCCCAGCCCCCTGATGCTGTTCTGTCATCAGGCGa                                                             <  2:377276/93‑1 (MQ=255)

                             cGCTGGCAGAGGCAGAAAGTTTGGCGCAAACCTGCTCCATATTGCTGATGATCGCCGCCCCCAGCCCCCTGATGCTGTTCTGTCATCAGGCGa                                                             <  2:1772845/93‑1 (MQ=255)

                               cTGGCAGAGGCAGAAAGTTTGGCGCAAACCTGCTCCATATTGCTGATGATCGCCGCCCCCAGCCCCCTGATGCTGTTCTGTCATCAGGCGACg                                                           >  2:2002681/1‑93 (MQ=255)

                               cTGGCAGAGGCAGAAAGTTTGGCGCAAACCTGCTCCATATTGCTGATGATCGCCGCCCCCAGCCCCCTGATGCTGTTCTGTCATCAGGCGACg                                                           >  1:593977/1‑93 (MQ=255)

                                 ggcagaggcagaAAGTTTGGCGCAAACCTGCTCCATATTGCTGATGATCGCCGCCCCCAGCCCCCTGATGCTGTTCTGTCATCAGGCGACGGa                                                         <  1:2428899/93‑1 (MQ=255)

                                   cagaggcagaAAGTTTGGCGCAAACCTGCTCCATATTGCTGATGATCGCCGCCCCCAGCCCCCTGATGCTGTTCTGTCATCAGGCGACGGata                                                       <  1:2874673/93‑1 (MQ=255)

                                    agaggcagaAAGTTTGGCGCAAACCTGCTCCATATTGCTGATGATCGCCGCCCCCAGCCCCCTGATGCTGTTCTGTCATCAGGCGACGGatat                                                      >  1:2289049/1‑93 (MQ=255)

                                    agaggcagaAAGTTTGGCGCAAACCTGCTCCATATTGCTGATGATCGCCGCCCCCAGCCCCCTGATGCTGTTCTGTCATCAGGCGACGGatat                                                      <  2:4467144/93‑1 (MQ=255)

                                     gaggcagaAAGTTTGGCGCAAACCTGCTCCATATTGCTGATGATCGCCGCCCCCAGCCCCCTGATGCTGTTCTGTCATCAGGCGACGGATAtt                                                     >  1:4074977/1‑93 (MQ=255)

                                     gaggcagaAAGTTTGGCGCAAACCTGCTCCATATTGCTGATGATCGCCGCCCCCAGCCCCCTGATGCTGTTCTGTCATCAGGCGACGGATAtt                                                     >  1:235115/1‑93 (MQ=255)

                                     gaggcagaAAGTTTGGCGCAAACCTGCTCCATATTGCTGATGATCGCCGCCCCCAGCCCCCTGATGCTGTTCTGTCATCAGGCGACGGATAtt                                                     >  2:3935330/1‑93 (MQ=255)

                                       ggcagaAAGTTTGGCGCAAACCTGCTCCATATTGCTGATGATCGCCGCCCCCAGCCCCCTGATGCTGTTCTGTCATCAGGCGACGGATATTGc                                                   >  1:2527376/1‑93 (MQ=255)

                                       ggcagaAAGTTTGGCGCAAACCTGCTCCATATTGCTGATGATCGCCGCCCCCAGCCCCCTGATGCTGTTCTGTCATCAGGCGACGGATATTGc                                                   >  2:1657616/1‑93 (MQ=255)

                                       ggcagaAAGTTTGGCGCAAACCTGCTCCATATTGCTGATGATCGCCGCCCCCAGCCCCCTGATGCTGTTCTGTCATCAGGCGACGGATATTGc                                                   <  1:3550425/93‑1 (MQ=255)

                                        gcagaAAGTTTGGCGCAAACCTGCTCCATATTGCTGATGATCGCCGCCCCCAGCCCCCTGATGCTGTTCTGTCATCAGGCGACGGATATTGcc                                                  <  1:310799/93‑1 (MQ=255)

                                        gcagaAAGTTTGGCGCAAACCTGCTCCATATTGCTGATGATCGCCGCCCCCAGCCCCCTGATGCTGTTCTGTCATCAGGCGACGGATATTGcc                                                  >  1:888621/1‑93 (MQ=255)

                                        gcagaAAGTTTGGCGCAAACCTGCTCCATATTGCTGATGATCGCCGCCCCCAGCCCCCTGATGCTGTTCTGTCATCAGGCGACGGATATTGcc                                                  <  1:949393/93‑1 (MQ=255)

                                         cagaAAGTTTGGCGCAAACCTGCTCCATATTGCTGATGATCGCCGCCCCCAGCCCCCTGATGCTGTTCTGTCATCAGGCGACGGATATTGCCt                                                 >  1:1873038/1‑93 (MQ=255)

                                         cagaAAGTTTGGCGCAAACCTGCTCCATATTGCTGATGATCGCCGCCCCCAGCCCCCTGATGCTGTTCTGTCATCAGGCGACGGATATTGCCt                                                 <  2:1134869/93‑1 (MQ=255)

                                         cagaAAGTTTGGCGCAAACCTGCTCCATATTGCTGATGATCGCCGCCCCCAGCCCCCTGATGCTGTTCTGTCATCAGGCGACGGATATTGCCt                                                 >  2:825093/1‑93 (MQ=255)

                                          agaAAGTTTGGCGCAAACCTGCTCCATATTGCTGATGATCGCCGCCCCCAGCCCCCTGATGCTGTTCTGTCATCAGGCGACGGATATTGCCTg                                                >  2:4208024/1‑93 (MQ=255)

                                           gaAAGTTTGGCGCAAACCTGCTCCATATTGCTGATGATCGCCGCCCCCAGCCCCCTGATGCTGTTCTGTCATCAGGCGACGGATATTGCCTGc                                               >  1:243520/1‑93 (MQ=255)

                                           gaAAGTTTGGCGCAAACCTGCTCCATATTGCTGATGATCGCCGCCCCCAGCCCCCTGATGCTGTTCTGTCATCAGGCGACGGATATTGCCTGc                                               >  2:4471960/1‑93 (MQ=255)

                                           gaAAGTTTGGCGCAAACCTGCTCCATATTGCTGATGATCGCCGCCCCCAGCCCCCTGATGCTGTTCTGTCATCAGGCGACGGATATTGCCTGc                                               >  1:770165/1‑93 (MQ=255)

                                           gaAAGTTTGGCGCAAACCTGCTCCATATTGCTGATGATCGCCGCCCCCAGCCCCCTGATGCTGTTCTGTCATCAGGCGACGGATATTGCCTGc                                               >  2:1634696/1‑93 (MQ=255)

                                            aaaGTTTGGCGCAAACCTGCTCCATATTGCTGATGATCGCCGCCCCCAGCCCCCTGATGCTGTTCTGTCATCAGGCGACGGATATTGCCTGCa                                              >  2:2682443/1‑93 (MQ=255)

                                            aaaGTTTGGCGCAAACCTGCTCCATATTGCTGATGATCGCCGCCCCCAGCCCCCTGATGCTGTTCTGTCATCAGGCGACGGATATTGCCTGCa                                              >  2:2529360/1‑93 (MQ=255)

                                              aGTTTGGCGCAAACCTGCTCCATATTGCTGATGATCGCCGCCCCCAGCCCCCTGATGCTGTTCTGTCATCAGGCGACGGATATTGCCTGCaaa                                            >  1:1603224/1‑93 (MQ=255)

                                               gTTTGGCGCAAACCTGCTCCATATTGCTGATGATCGCCGCCCCCAGCCCCCTGATGCTGTTCTGTCATCAGGCGACGGATATTGCCTGCAAAt                                           <  2:2973473/93‑1 (MQ=255)

                                                tttGGCGCAAACCTGCTCCATATTGCTGATGATCGCCGCCCCCAGCCCCCTGATGCTGTTCTGTCATCAGGCGACGGATATTGCCTGCAAAtt                                          <  2:949584/93‑1 (MQ=255)

                                                  tGGCGCAAACCTGCTCCATATTGCTGATGATCGCCGCCCCCAGCCCCCTGATGCTGTTCTGTCATCAGGCGACGGATATTGCCTGCAAATtca                                        >  2:3613504/1‑93 (MQ=255)

                                                     cgcAAACCTGCTCCATATTGCTGATGATCGCCGCCCCCAGCCCCCTGATGCTGTTCTGTCATCAGGCGACGGATATTGCCTGCAAATtcatca                                     <  2:4437053/93‑1 (MQ=255)

                                                        aaaCCTGCTCCATATTGCTGATGATCGCCGCCCCCAGCCCCCTGATGCTGTTCTGTCATCAGGCGACGGATATTGCCTGCAAATTCATCATcc                                  <  1:2663038/93‑1 (MQ=255)

                                                        aaaCCTGCTCAATATTGCTGATGATCGCCGCCCCCAGCCCCCTGATGCTGTTCTGTCATCAGGCGACGGATATTGCCTGCAAATTCATCATcc                                  <  2:150855/93‑1 (MQ=255)

                                                         aaCCTGCTCCATATTGCTGATGATCGCCGCCCCCAGCCCCCTGATGCTGTTCTGTCATCAGGCGACGGATATTGCCTGCAAATTCATCATCCt                                 >  1:4145490/1‑93 (MQ=255)

                                                            cTGCTCCATATTGCTGATGATCGCCGCCCCCAGCCCCCTGATGCTGTTCTGTCATCAGGCGACGGATATTGCCTGCAAATTCATCATCCTGaa                              >  2:2277438/1‑93 (MQ=255)

                                                            cTGCTCCATATTGCTGATGATCGCCGCCCCCAGCCCCCTGATGCTGTTCTGTCATCAGGCGACGGATATTGCCTGCAAATTCATCATCCTGaa                              <  2:2386043/93‑1 (MQ=255)

                                                              tcTCCATATTGCTGATGATCGCCGCCCCCAGCCCCCTGATGCTGTTCTGTCATCAGGCGACGGATATTGCCTGCAAATTCATCATCCTGAATc                            <  1:190936/92‑1 (MQ=255)

                                                                tCCATATTGCTGATGATCGCCGCCCCCAGCCCCCTGATGCTGTTCTGTCATCAGGCGACGGATATTGCCTGCAAATTCATCATCCTGAATCAg                          >  1:1016007/1‑93 (MQ=255)

                                                                tCCATATTGCTGATGATCGCCGCCCCCAGCCCCCTGATGCTGTTCTGTCATCAGGCGACGGATATTGCCTGCAAATTCATCATCCTGAATCAg                          >  1:4521426/1‑93 (MQ=255)

                                                                 ccATATTGCTGATGATCGCCGCCCCCAGCCCCCTGATGCTGTTCTGTCATCAGGCGACGGATATTGCCTGCAAATTCATCATCCTGAATCAGc                         <  1:90408/93‑1 (MQ=255)

                                                                  cATATTGCTGATGATCGCCGCCCCCAGCCCCCTGATGCTGTTCTGTCATCAGGCGACGGATATTGCCTGCAAATTCATCATCCTGAATCAGCg                        >  2:584025/1‑93 (MQ=255)

                                                                  cATATTGCTGATGATCGCCGCCCCCAGCCCCCTGATGCTGTTCTGTCATCAGGCGACGGATATTGCCTGCAAATTCATCATCCTGAATCAGCg                        >  1:1769475/1‑93 (MQ=255)

                                                                   atatTGCTGATGATCGCCGCCCCCAGCCCCCTGATGCTGTTCTGTCATCAGGCGACGGATATTGCCTGCAAATTCATCATCCTGAATCAGCGa                       >  2:3546453/1‑93 (MQ=255)

                                                                    tatTGCTGATGATCGCCGCCCCCAGCCCCCTGATGCTGTTCTGTCATCAGGCGACGGATATTGCCTGCAAATTCATCATCCTGAATCAGCGAc                      >  2:1104228/1‑93 (MQ=255)

                                                                      ttGCTGATGATCGCCGCCCCCAGCCCCCTGATGCTGTTCTGTCATCAGGCGACGGATATTGCCTGCAAATTCATCATCCTGAATCAGCGACaa                    >  1:1968265/1‑93 (MQ=255)

                                                                      ttGCTGATGATCGCCGCCCCCAGCCCCCTGATGCTGTTCTGTCATCAGGCGACGGATATTGCCTGCAAATTCATCATCCTGAATCAGCGACaa                    <  1:2585991/93‑1 (MQ=255)

                                                                       tGCTGATGATCGCCGCCCCCAGCCCCCTGATGCTGTTCTGTCATCAGGCGACGGATATTGCCTGCACAATCATCATCCTGAATCAGCGACaaa                   >  2:3552927/1‑93 (MQ=255)

                                                                       tGCTGATGATCGCCGCCCCCAGCCCCCTGATGCTGTTCTGTCATCAGGCGACGGATATTGCCTGCAAATTCATCATCCTGAATCAGCGACaaa                   >  1:3895505/1‑93 (MQ=255)

                                                                        gCTGATGATCGCCGCCCCCAGCCCCCTGATGCTGTTCTGTCATCAGGCGACGGATATTGCCTGCAAATTCATCATCCTGAATCAGCGACAAAt                  <  1:1801479/93‑1 (MQ=255)

                                                                         cTGATGATCGCCGCCCCCAGCCCCCTGATGCTGTTCTGTCATCAGGCGACGGATATTGCCTGCAAATTCATCATCCTGAATCAGCGACAAATg                 <  1:4341042/93‑1 (MQ=255)

                                                                          tgatgaTCGCCGCCCCCAGCCCCCTGATGCTGTTCTGTCATCAGGCGACGGATATTGCCTGCAAATTCATCATCCTGAATCAGCGACAAATgg                <  1:1052382/93‑1 (MQ=255)

                                                                          tgatgaTCGCCGCCCCCAGCCCCCTGATGCTGTTCTGACATCAGGCGACGGATATTGCCTGCAAATTCATCATCCTGAATCAGCGACAAATgg                >  1:2098922/1‑93 (MQ=255)

                                                                          tgatgaTAGCCGCCCCCAGCCCCCTGATGCTGTTCTGTCATCAGGCGACGGATATTGCCTGCAAATTCATCATCCTGAATAAGCGACAAATgg                >  2:1756501/1‑93 (MQ=255)

                                                                           gatgatCGCCGCCCCCAGCCCCCTGATGCTGTTCTGTCATCAGGCGACGGATATTGCCTGCAAATTCATCATCCTGAATCAGCGACAAATggg               <  1:646303/93‑1 (MQ=255)

                                                                            atgatCGCCGCCCCCAGCCCCCTGATGCTGTTCTGTCATCAGGCGACGGATATTGCCTGCAAATTCATCATCCTGAATCAGCGACAAATGGgc              <  1:2700143/93‑1 (MQ=255)

                                                                               atCGCCGCCCCCAGCCCCCTGATGCTGTTCTGTCATCAGGCGACGGATATTGCCTGCAAATTCATCATCCTGAATCAGCGACAAATGGGCGCt           >  2:4674551/1‑93 (MQ=255)

                                                                                tCGCCGCCCCCAGCCCCCTGATGCTGTTCTGTCATCAGGCGACGGATATTGCCTGCAAATTCATCATCCTGAATCAGCGACAAATGGGCGCTg          >  1:866525/1‑93 (MQ=255)

                                                                                 cgccgcCCCCAGCCCCCTGATGCTGTTCTGTCATCAGGCGACGGATATTGCCTGCAAATTCATCATCCTGAATCAGCGACAAATGGGCGCTGa         <  2:4335832/93‑1 (MQ=255)

                                                                                    cgccCCCAGCCCCCTGATGCTGTTCTGTCATCAGGCGACGGATATTGCCTGCAAATTCATCATCCTGAATCAGCGACAAATGGGCGCTGAGGa      <  2:2572772/93‑1 (MQ=255)

                                                                                     gccCCCAGCCCCCTGATGCTGTTCTGTCATCAGGCGACGGATATTGCCTGCAAATTCATCATCCTGAATCAGCGACAAATGGGCGCTGAGGAt     <  1:1179316/93‑1 (MQ=255)

                                                                                     gccCCCAGCCCCCTGATGCTGTTCTGTCATCAGGCGACGGATATTGCCTGCAAATTCATCATCCTGAATCAGCGACAAATGGGCGCTGAGGAt     <  2:132757/93‑1 (MQ=255)

                                                                                     gccCCCAGCCCCCTGATGCTGTTCTGTCATCAGGCGACGGATATTGCCTGCAAATTCATCATCCTGAATCAGCGACAAATGGGCGCTGAGGAt     <  1:3939730/93‑1 (MQ=255)

                                                                                      cccccAGCCCCCTGATGCTGTTCTGTCATCAGGCGACGGATATTGCCTGCAAATTCATCATCCTGAATCAGCGACAAATGGGCGCTGAGGATa    <  2:4412757/93‑1 (MQ=255)

                                                                                        cccAGCCCCCTGATGCTGTTCTGTCATCAGGCGACGGATATTGCCTGCACATTCATCATCCTGAATCAGCGACAAATGGGCGCTGAGGATAGt  >  1:2546310/1‑93 (MQ=255)

                                                                                        cccAGCCCCCTGATGCTGTTCTGTCATCAGGCGACGGATATTGCCTGCAAATTCATCATCCTGAATCAGCGACAAATGGGCGCTGAGGATAGt  <  1:2254965/93‑1 (MQ=255)

                                                                                        cccAGCCCCCTGATGCTGTTCTGTCATCAGGCGACGGATATTGCCTGCAAATTCATCATCCTGAATCAGCGACAAATGGGCGCTGAGGATAGt  <  2:2508418/93‑1 (MQ=255)

                                                                                        cccAGCCCCCTGATGCTGTTCTGTCATCAGGCGACGGATATTGCCTGCAAATTCATCATCCTGAATCAGCGACAAATGGGCGCTGAGGATAGt  >  2:3503375/1‑93 (MQ=255)

AATGTCGCTAACACGTTCGCGCAGATAATCGCTGGCAGAGGCAGAAAGTTTGGCGCAAACCTGCTCCATATTGCTGATGATCGCCGCCCCCAG‑CCCCTGATGCTGTTCTGTCATCAGGCGACGGATATTGCCTGCAAATTCATCATCCTGAATCAGCGACAAATGGGCGCTGAGGATAGT  >  NC_000913/2501792‑2501971

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 24 ≤` `ATCG/ATCG` `< 31 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`