Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 280,274 (G)5→6 coding (934/1155 nt) yagA ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913280,2691.G97.8% 421.0 / 2.1 137coding (939/1155 nt)yagAhypothetical protein
Reads supporting (aligned to +/- strand):  ref base . (1/2);  new base G (73/61);  total (74/63)
Fisher's exact test for biased strand distribution p-value = 5.94e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.16e-01

Only 100 of 166 total aligned reads displayed.

CTGAACGCCTTGCCTGCGCTCAGACTTACCCCTTTCACGCTCAGCTTTCCGCTGATATCCACTTTCCTGACCATCACCCCTTCATCGTATTCC‑G‑GGGGCGTTGTGTTGCCGCTGTACTGCCGCGCTGACGGCTGATACCGCGAGCCCGGTACCGCCATATCCAGCGCCTCGTGCGGGCGTTCAAG  >  NC_000913/280177‑280361
                                                                                             |                                                                                             
cTGAACGCCTTGCCTGCGCTCAGACTTACCCCTTTCACGCTCAGCTTTCCGCTGATATCCACTTTCCTGACCATCACCCCTTCATCGTATTcc                                                                                                <  1:1177437/93‑1 (MQ=255)
cTGAACGCCTTGCCTGCGCTCAGAATTACCCCTTTCACGCTCAGCTTTCCGCTGATATCCACTTTCCCGAGCATCACCCGTGCACCGGATTcc                                                                                                >  2:3032751/1‑93 (MQ=255)
 tGAACGCCTTGCCTGCGCTCAGACTTACCCCTTTCACGCTCAGCTTTCCGCTGATATCCACTTTCCTGACCATCACCCCTTCATCGTATTCC‑g                                                                                              <  2:3009912/93‑1 (MQ=255)
  gAACGCCTTGCCTGCGCTCAGACTTCCCCCTTTCACGCTCAGCTTTCCGCTGATATCCACTTTCCTGACCATCACCCCTTCATCGTATTCC‑g‑g                                                                                            <  2:2822318/93‑1 (MQ=255)
  gAACGCCTTGCCTGCGCTCAGACTTACCCCTTTCACGCTCAGCTTTCCGCTGATATCCACTTTCCTGACCATCACCCCTTCATCGTATTCC‑g‑g                                                                                            <  2:2151920/93‑1 (MQ=255)
  gAACGCCTTGCCTGCGCTCAGACTTACCCCTTTCACGCTCAGCTTTCCGCTGATATCCACTTTCCTGACCATCACCCCTTCATCGTATTCC‑g‑g                                                                                            <  2:2151919/93‑1 (MQ=255)
  gAACGCCTTGCCTGCGCTCAGACTTACCCCTTTCACGCTCAGCTTTCCGCTGATATCCACTTTCCTGACCATCACCCCTTCATCGTATTCC‑g‑g                                                                                            <  1:3196327/93‑1 (MQ=255)
    aCGCCTTGCCTGCGCTCAGACTTACCCCTTTCACGCTCAGCTTTCCGCTGATATCCACTTTCCTGACCATCACCCCTTCATCGTATTCC‑g‑ggg                                                                                          <  1:1214786/93‑1 (MQ=255)
    aCGCCTTGCCTGCGCTCAGACTTACCCCTTTCACGCTCAGCTTTCCGCTGATATCCACTTTCCTGACCATCACCCCTTCATCGTATTCC‑g‑ggg                                                                                          >  2:1882621/1‑93 (MQ=255)
      gCCTTGCCTGCGCTCAGACTTACCCCTTTCACGCTCAGCTTTCCGCTGATATCCACTTTCCTGACCATCACCCCTTCATCGTATTCC‑g‑ggggg                                                                                        >  2:2404301/1‑92 (MQ=255)
        cTTGCCTGCGCTCAGACTTACCCCTTTCACGCTCAGCTTTCCGCTGATATCCACTTTCCTGACCATCACCCCTTCATCGTATTCC‑g‑gggggcg                                                                                      >  2:3394091/1‑90 (MQ=255)
        cTTGCCTGCGCTCAGACTTACCCCTTTCACGCTCAGCTTTCCGCTGATATCCACTTTCCTGACCATCACCCCTTCATCGTATTCC‑g‑gggggcg                                                                                      >  2:4175207/1‑90 (MQ=255)
         ttGCCTGCGCTCAGACTTCCCCCTTTCACGCTCAGCTTTCCGCTGATATCCACTTTCCTGACCATCACCCCTTCATCGTATTCC‑g‑gggggcgt                                                                                     <  1:1861471/93‑5 (MQ=255)
           gCCTGCGCTCAGACTTACCCCTTTCACGCTCAGCTTTCCGCTGATATCCACTTTCCTGACCATCACCCCTTCATCGTATTCCGG‑GGGGCGTtg                                                                                    <  2:464983/93‑1 (MQ=255)
               gcgcTCAGACTTACCCCTTTCACGCTCAGCTTTCCGCTGATATCCACTTTCCTGACCATCACCCCTTCATCGTATTCCGG‑GGGGCGTTGTGtt                                                                                <  2:3954971/93‑1 (MQ=255)
               gcgcTCAGACTTACCCCTTTCACGCTCAGCTTTCCGCTGATATCCACTTTCCTGACCATCACCCCTTCATCGTATTCCGG‑GGGGCGTTGTGtt                                                                                <  2:1897973/93‑1 (MQ=255)
               gcgcTCAGACTTACACCTTTCACGCTCAGCTTTCCGCTGATATCCACTTTCCTGACCATCACCCCTTCATCGTATTCCGG‑GGGGCGTTGTGtt                                                                                <  1:3950177/93‑1 (MQ=255)
                 gcTCAGACTTACCCCTTTCTCGCTCAGCTTTCCGCTGATATCCACTTTCCTGACCATCACCCCTTCATCGTATTCCGG‑GGGGCGTTGTGTTGc                                                                              >  2:4572266/1‑93 (MQ=255)
                    cAGACTTACCCCTTTCACGCTCAGCTTTCCGCTGATATCCACTTTCCTGACCATCACCCCTTCATCGTATTCCGG‑GGGGCGTTGTGTTGCCGc                                                                           <  2:3809581/93‑1 (MQ=255)
                    cAGACTTACCCCTTTCACGCTCAGCTTTCCGCTGATATCCACTTTCCTGACCATCACCCCTTCATCGTATTCCGG‑GGGGCATTGTGTTGCCGc                                                                           >  2:1418303/1‑93 (MQ=255)
                      gACTTACCCCTTTCACGCTCAGCTTTCCGCTGATATCCACTTTCCTGACCATCACCCCTTCATCGTATTCCGG‑GGGGCGTTGTGTTGCCGCTg                                                                         >  1:1699603/1‑93 (MQ=255)
                      gACTTACCCCTTTCACGCTCAGCTTTCCGCTGATATCCACTTTCCTGACCATCACCCCTTCATCGTATTCCGG‑GGGGCGTTGTGTTGCCGCTg                                                                         >  1:910377/1‑93 (MQ=255)
                         ttACCCCTTTCACGCTCAGCTTTCCGCTGATATCCACTTTCCTGACCATCACCCCTTCATCGTATTCCGG‑GGGGCGTTGTGTTGCCGCTGTAc                                                                      >  1:3052438/1‑93 (MQ=255)
                         ttACCCCTTTCACGCTCAGCTTTCCGCTGATATCCACTTTCCTGACCATCACCCCTTCATCGTATTCCGG‑GGGGCGTTGTGTTGCCGCTGTAc                                                                      <  1:2439080/93‑1 (MQ=255)
                          tACCCCTTTCACGCTCAGCTTTCCGCTGATATCCACTTTCCTGACCATCACCCCTTCATCGTATTCCGG‑GGGGCGTTGTGTTGCCGCTGTACt                                                                     <  1:70213/93‑1 (MQ=255)
                            ccccTTTCACGCTCAGCTTTCCGCTGATATCCACTTTCCTGACCATCACCCCTTCATCGTATTCCGG‑GGGGCGTTGTGTTGCCGCTGTACTGc                                                                   >  2:2037192/1‑93 (MQ=255)
                             cccTTTCACGCTCAGCTTTCCGCTGATATCCCCTTTCCTGACCATCACCCCTTCATCGTATTCC‑GTGGGGCGTTGTGTTGCCGCTGTACTGcc                                                                  >  2:531450/1‑93 (MQ=255)
                             cccTTTCACGCTCAGCTTTCCGCTGATATCCACTTTCCTGACCATCACCCCTTCATCGTATTCCGG‑GGGGCGTTGTGTTGCCGCTGTACTGcc                                                                  >  1:3491278/1‑93 (MQ=255)
                             cccTTTCACGCTCAGCTTTCCGCTGATATCCACTTTCCTGACCATCACCCCTTCATCGTATTCCGG‑GGGGCGTTGTGTTGCCGCTGTACTGcc                                                                  >  1:4559195/1‑93 (MQ=255)
                             cccTTTCACGCTCAGCTTTCCGCTGATATCCACTTTCCTGACCATCACCCCTTCATCGTATTCCGG‑GGGGCGTTGTGTTGCCGCTGTACTGcc                                                                  <  2:3484100/93‑1 (MQ=255)
                              ccTTTCACGCTCAGCTTTCCGCTGATATGCACTTTCCTGACCATCAACCCTTCATCGTATTCC‑GAGGGGCGTTGTGCTGCCGCTGTACTGCcg                                                                 >  2:1309404/1‑93 (MQ=255)
                              ccTTTCACGCTCAGCTTTCCGCTGATATCCACTTTCCTGACCATCACCCCTTCATCGTATTCCGG‑GGGGCGTTGTGTTGCCGCTGTCCTGCcg                                                                 >  2:1034791/1‑93 (MQ=255)
                              ccTTTCACGCTCAGCTTTCCGCTGATATCCACTTTCCTGACCATCACCCCTTCATCGTATTCCGG‑GGGGCGTTGTGTTGCCGCTGTACTGCcg                                                                 <  2:1594731/93‑1 (MQ=255)
                              ccTTTCACGCTCAGCTTTCCGCTGATATCCACTTTCCTGACCATCACCCCTTCATCGTATTCCGG‑GGGGCGTTGTGTTGCCGCTGTACTGCcg                                                                 >  1:1049693/1‑93 (MQ=255)
                               cTTTCACGCTCAGCTTTCCGCTGATATCCACTTTCCTGACCATCACCCCTTCATCGTATTCCGG‑GGGGCGTTGTGTTGCCGCTGTACTGCcgc                                                                <  1:2652085/93‑1 (MQ=255)
                                tttCACGCTCAGCTTTCCGCTGATATCCACTTTCCTGACCATCACCCCTTCATCGTATTCCGG‑GGGGCGTTGTGTTGCCGCTGTACTGCcgcg                                                               >  1:472024/1‑93 (MQ=255)
                                 ttCACGCTCAGCTTTCCGCTGATATCCACTTTCCTGACCATCACCCCTTCATCGTATTCCGG‑GGGGCGTTGTGTTGCCGCTGTACTGCCgcgc                                                              >  2:4540052/1‑93 (MQ=255)
                                     cGCTCAGCTTTCCGCTGATATCCACTTTCCTGACCATCACCCCTTCATCGTATTCCGG‑GGGGCGTTGTGTTGCCGCTGTACTGCCGCGCTGAc                                                          <  2:1435849/93‑1 (MQ=255)
                                     cGCTCAGCTTTCCGCTGATATCCACTTTCCTGACCATCACCCCTTCATCGTATTCCGG‑GGGGCGTTGTGTTGCCGCTGTACTGCCGCGCTGAc                                                          <  2:4135085/93‑1 (MQ=255)
                                      gCTCCGCTTTCCGCTGATATCCACTTTCCTGACCATCACCCCTTCATCGTATTCCGG‑GGGGCGTTGTGTTGCCGCTGTACTTCCGCGCTGACg                                                         >  2:1957078/1‑93 (MQ=255)
                                      gCTCAGCTTTCCGCTGATATCCACTTTCCTGAACATCACCCCTTCATCGTATTCCGG‑GGGGCGTTGTGTTGCCGCTGTACTGCCGCGCTGACg                                                         <  2:2448937/93‑1 (MQ=255)
                                       cTCAGCTTTCCGCTGATATCCACTTTCCTGACCATCACCCCTTCATCGTATTCCGG‑GGGGCGTTGTGTTGCCGCTGTACTGCCGCGCTGACgg                                                        >  1:4631308/1‑93 (MQ=255)
                                        tCAGCTTTCCGCTGATATCCACTTTCCTGACCATCACCCCTTCATCGTATTCCGG‑GGGGCGTTGTGTTGCCGCTGTACCGCCGCGCTGACGGc                                                       <  2:2864090/93‑1 (MQ=255)
                                         cAGCTTTCCGCTGATATCCACTTTCCTGACCATCACCCCTTCATCGTATTCCGG‑GGGGCGTTGTGTTGCCGCTGTACTGCCGCGCTGACGGCt                                                      <  2:3124257/93‑1 (MQ=255)
                                           gCTTTCCGCTGATATCCACTTTCCTGACCATCACCCCTTCATCGTATTCCGG‑GGGGCGTTGTGTTGCCGCTGTACTGCCGCGCTGACGGCTGa                                                    <  2:764638/93‑1 (MQ=255)
                                             tttCCGCTGATATCCACTTTCCTGACCATCACCCCTTCATCGTATTCCGG‑GGGGCGTTGTGTTGCCGCTGTACTGCCGCGCTGACGGCTGATa                                                  <  1:2869776/93‑1 (MQ=255)
                                                ccGCTGATATCCACTTTCCTGACCATCACCCCTTCATCGTATTCCGG‑GGGGCGTTGTGTTGCCGCTGTACTGCCGCGCTGACGGCTGATACcg                                               >  1:527929/1‑93 (MQ=255)
                                                ccGCTGATATCCACTTTCCTGACCATCACCCCTTCATCGTATTCCGG‑GGGGCGTTGTGTTGCCGCTGTACTGCCGCGCTGACGGCTGATACcg                                               >  1:3108832/1‑93 (MQ=255)
                                                ccGCTGATATCCACTTTCCTGACCATCACCCCTTCATCGTATTCCGG‑GGGGCGTTGTGTTGCCGCTGTACTGCCGCGCTGACGGCTGATACcg                                               <  2:534159/93‑1 (MQ=255)
                                                  gCTGATATCCACTTTCCTGACCATCACCCCTTCATCGTATTCCGG‑GGGGCGTTGTGTTGCCGCTGTACTGCCGCGCTGACGGCTGATACcgcg                                             >  1:2414993/1‑93 (MQ=255)
                                                   cTGATATCCACTTTCCTGACCATCACCCCTTCATCGTATTCCGG‑GGGGCGTTGTGTTGCCGCTGTACTGCCGCGCTGACGGCTGATACCGCGa                                            >  1:634292/1‑93 (MQ=255)
                                                     gATATCCACTTTCCTGACCATCACCCCTTCATCGTATTCCGG‑GGGGCGTTGTGTTGCCGCTGTACTGCCGCGCTGACGGCTGATACCGCGAGc                                          >  1:1929329/1‑93 (MQ=255)
                                                     gATATCCACTTTCCTGACCATCACCCCTTCATCGTATTCCGG‑GGGGCGGTGTGTTGCCGCTGTACTGCCGCGCTGACGGCTGATAACGCGAGc                                          >  2:1829934/1‑93 (MQ=255)
                                                     gATATCCACTTTCCTGACCATCACCCCTTCATCGTATTCCGG‑GGGGCGCTGTGTTGCCGCTGTACTGCCGCGCTGACGTCTGATCCCGCGAGc                                          >  2:9383/1‑93 (MQ=255)
                                                       tatCCACTTTCCTGACCATCACCCCTTCATCGTATTCCGG‑GGGGCGTTGTGTTGCCGCTGTACTGCCGCGCTGACGGCTGATACCGCGAGccc                                        <  2:852167/93‑1 (MQ=255)
                                                          ccACTTTCCTGACCATCACCCCTTCATCGTATTCCGG‑GGGGCGTTGTGTTGCCGCTGTACTGCCGCGCTGACGGCTGATACCGCGAGCCCGGt                                     >  1:556322/1‑93 (MQ=255)
                                                           cACTTTCCTGACCATCACCCCTTCATCGTATTCCGG‑GGGGCGTTGTGTTGCCGCTGTACTGCCGCGCTGACGGCTGATACCGCGAGCCCGGTa                                    <  2:941226/93‑1 (MQ=255)
                                                            ccTTTCCTGACCATCACCCCTTCATCGTATTCCGG‑GGGGCGTTGTGTTGCCGCTGTACTGCCGCGCTGACGGCTGATACCGCGAGCCCGGTAc                                   <  1:2343795/92‑1 (MQ=255)
                                                              tttCCTGACCATCACCCCTTCATCGTATTCCGG‑GGGGCGTTGTGTTGCCGCTGTACTGCCGCGCTGCCGGCGGATACCGCGGGCCCGGTACCg                                 >  1:838832/1‑93 (MQ=255)
                                                              tttCCTGACCATCACCCCTTCATCGTATTCCGG‑GGGGCGTTGTGTTGCCGCTGTACTGCCGCGCTGACGGCTGATACCGCGAGCCCGGTACCg                                 <  1:2589276/93‑1 (MQ=255)
                                                              tttCCTGACCATCACCCCTTCATCGTATTCCGG‑GGGGCGTTGTGTTGCCGCTGTACTGCCGCGCTGACGGCTGATACCGCGAGCCCCGCACCg                                 >  1:1174957/1‑93 (MQ=255)
                                                                 ccTGACCATCCCCCCTTCATCGTATTCCGG‑GGGGCGTTGTGTTGCCGCTGTACTGCCGCGCTGACGGCTGATACCGCGAGCCCGGTACCGCCa                              <  1:4098360/93‑1 (MQ=255)
                                                                 ccTGACCATCACCCCTTCATCGTATTCCGG‑GGGGCGTTGTGTTGCCGCTGTACTGCCGCGCTGACGGCTGATACCGCGAGCCCGGTACCGCCa                              >  1:3761383/1‑93 (MQ=255)
                                                                 ccTGACCATCACCCCTTCATCGTATTCCGG‑GGGGCGTTGTGTTGCCGCTGTACTGCCGCGCTGACGGCTGATACCGCGAGCCCGGTACCGCCa                              >  1:2111676/1‑93 (MQ=255)
                                                                 ccTGACCATCACCCCTTCATCGTATTCCGG‑GGGGCGTTGTGTTGCCGCTGTACTGCCGCGCTGACGGCTGATACCGCGAGCCCGGTACCGCCa                              <  1:4335962/93‑1 (MQ=255)
                                                                   tGACCATCACCCCTTCATCGTATTCCGG‑GGGGCGTTGTGTTGCCGCTGTACTGCCGCGCTGACGGCTGATACCGCGAGCCCGGTACCGCCata                            <  2:61726/93‑1 (MQ=255)
                                                                   cgACCATCCCCCCTTCATCGTATTCCGG‑GGGGCGTTGTGTTGCCGCTGTACTGCCGCGCTGACGGCTGATACCGCGAGCCCGGTACCGCCata                            <  2:1316990/92‑1 (MQ=255)
                                                                     aCCATCACCCCTTCATCGTATTCCGG‑GGGGCGTTGTGTTGCCGCTGTACTGCCGCGCTGACGGCTGATACCGCGAGCCCGGTACCGCCATATc                          <  2:960170/93‑1 (MQ=255)
                                                                       cATCACCCCTTCATCGTATTCCGG‑GGGGCGTTGTGTTGCCGCTGTACTGCCGCGCTGACGGCTGATACCGCGAGCCCGGTACCGCCATATCCa                        >  1:3635507/1‑93 (MQ=255)
                                                                       cATCACCCCTTCATCGTATTCCGG‑GGGGCGTTGTGTTGCCGCTGTACTGCCGCGCTGACGGCTGATACCGCGAGCCCGGTACCGCCATATCCa                        >  1:3666489/1‑93 (MQ=255)
                                                                        aTCACCCCTTCATCGTATTCCGG‑GGGGCGTTGTGTTGCCGCTGTACTGCCGCGCTGACGGCTGATACCGCGAGCCCGGTACCGCCATATCCAg                       >  2:191254/1‑93 (MQ=255)
                                                                         tCACCCCTTCATCGTATTCCGG‑GGGGCGTTGTGTTGCCGCTGTACTGCCGCGCTGACGGCTGATACCGCGAGCCCGGTACCGCCATATCCAgc                      <  2:2283986/93‑1 (MQ=255)
                                                                          cACCCCTTCATCGTATTCCGG‑GGGGCGTTGTGTTGCCGCTGTACTGCCGCGCTGACGGCTGATACCGCGAGCCCGGTACCGCCATATCCAgcg                     >  2:1671924/1‑93 (MQ=255)
                                                                          cACCCCTTCATCGTATTCCGG‑GGGGCGTTGTGTTGCCGCTGTACTGCCGCGCTGACGGCTGATACCGCGAGCCCGGTACCGCCATATCCAgcg                     >  2:3292619/1‑93 (MQ=255)
                                                                            ccccTTCATCGTATTCCGG‑GGGGCGTTGTGTTGCCGCTGTACTGCCGCGCTGACGGCTGATACCGCGAGCCCGGTACCGCCATATCCAGCGcc                   <  1:442409/93‑1 (MQ=255)
                                                                             cccTTCATCGTATTCCGG‑GGGGCGTTGTGTTGCCGCTGTACTGCCGCGCTGACGGCTGATACCGCGAGCCCGGTACCGCCATATCCAGCGCCt                  <  2:344108/93‑1 (MQ=255)
                                                                             cccTTCATCGTATTCCGG‑GGGGCGTTGTGTTGCCGCTGTACTGCCGCGCTGACGGCTGATACCGCGAGCCCGGTACCGCCATATCCAGCGCCt                  <  1:1879103/93‑1 (MQ=255)
                                                                             cccTTCATCGTATTCCGG‑GGGGCGTTGTGTTGCCGCTGTACTGCCGCGCTGACGGCTGATACCGCGAGCCCGGTACCGCCATATCCAGAGCCt                  >  2:1921334/1‑93 (MQ=255)
                                                                              ccTTCATCGTATTCCGG‑GGGGCGTTGTGTTGCCGCTGTACTGCCGCGCTGACGGCTGATACCGCGAGCCCGGTACCGCCATATCCAGCGCCTc                 <  1:1415788/93‑1 (MQ=255)
                                                                              ccTTCATCGTATTCCGG‑GGGGCGTTGTGTTGCCGCTGTACTGCCGCGCTGACGGCTGATACCGCGAGCCCGGTACCGCCATATCCAGCGCCTc                 >  1:1494546/1‑93 (MQ=255)
                                                                              ccTTCATCGTATTCCGG‑GGGGCGTTGTGTTGCCGCTGTACTGCCGCGCTGACGGCTGATACCGCGAGCCCGGTACCGCCATATCCAGCGCCTc                 >  1:1758435/1‑93 (MQ=255)
                                                                              ccTTCATCGTATTCCGG‑GGGGCGTTGTGTTGCCGCTGTACTGCCGCGCTGACGGCTGATACCGCGAGCCCGGTACCGCCATATCCAGCGCCTc                 <  2:3636133/93‑1 (MQ=255)
                                                                              ccTTCATCGTATTCCGG‑GGGGCGTTGTGTTGCCGCTGTACTGCCGCGCTGACGGCTGATACCGCGAGCCCGGTACCGCCATATCCAGCGCCTc                 >  2:3900273/1‑93 (MQ=255)
                                                                              ccTTCATCGTATTCCGG‑GGGGCGTTGTGTTGCCGCTGTACTGCCGCGCTGACGGCTGATACCGCGAGCCCGGTACCGCCATATCCAGCGCCTc                 <  2:744527/93‑1 (MQ=255)
                                                                                ttCATCGTATTCCGG‑GGGGCGTTGTGTTGCCGCTGTACTGCCGCGCTGACGGCTGATACCGCGAGCCCGGTACCGCCATATCCAGCGCCTCGt               >  1:2812842/1‑93 (MQ=255)
                                                                                ttCATCGTATTCCGG‑GGGGCGTTGTGTTGCCGCTGTACTGCCGCGCTGACGGCTGATACCGCGAGCCCGGTACCGCCATATCCAGCGCCTCGt               <  2:2776368/93‑1 (MQ=255)
                                                                                ttCATCGTATTCCGG‑GGGGCGTTGTGTTGCCGCTGTACTGCCGCGCTGACGGCTGATACCGCGAGCCCGGTACCGCCATATCCAGCGCCTCGt               >  1:4337350/1‑93 (MQ=255)
                                                                                 tCATCGTATTCCGG‑GGGGCGTTGTGTTGCCGCTGTACTGCCGCGCTGACGGCTGATACCGCGAGCCCGGTACCGCCATATCCAGCGCCTCGTg              <  1:1969692/93‑1 (MQ=255)
                                                                                 tCATCGTATTCCGG‑GGGGCGTTGTGTTGCCGCTGTACTGCCGCGCTGACGGCTGATACCGCGAGCCCGGTACCGCCATATCCAGCGCCTCGTg              >  1:4398187/1‑93 (MQ=255)
                                                                                 tCATCGTATTCCGG‑GGGGCGTTGTGTTGCCGCTGTACTGCCGCGCTGACGGCTGATACCGCGAGCCCGGTACCGCCATATCCAGCGCCTCGTg              >  2:1180952/1‑93 (MQ=255)
                                                                                  cATCGTATTCCGG‑GGGGCGTTGTGTTGCCGCTGTACTGCCGCGCTGACGGCTGATACCGCGAGCCCGGTACCGCCATATCCAGCGCCTCGTGc             <  2:4292358/93‑1 (MQ=255)
                                                                                  cATCGTATTCCGG‑GGGGCGTTGTGTTGCCGCTGTACTGCCGCGCTGACGGCTGATACCGCGAGCCCGGTACCGCCATATCCAGCGCCTCGTGc             <  1:2732423/93‑1 (MQ=255)
                                                                                  cATCGTATTCC‑G‑GGGGCGTTGTGTTGCCGCTGTACTGCCGCGCTGACGGCTGATACCGCGAGCCCGGTACCGCCATATCCAGCGCCTCGTGCg            >  1:2249127/1‑93 (MQ=255)
                                                                                    tCGTATTCCGG‑GGGGCGTTGTGTTGCCGCTGTACTGCCGCGCTGACGGCTGATACCGCGAGCCCGGTACCGCCATATCCAGCGCCTCGTGCgg           >  1:1902276/1‑93 (MQ=255)
                                                                                      gTATTCCGG‑GGGGCGTTGTGTTGCCGCTGTACTGCCGCGCTGACGGCTGATACCGCGAGCCCGGTACCGCCATATCCAGCGCCTCGTGCgggg         >  2:4240808/1‑92 (MQ=255)
                                                                                      gTATTCCGG‑GGGGCGTTGTGTTGCCGCTGTACTGCCGCGCTGACGGCTGATACCGCGAGCCCGGTACCGCCATATCCAGCGCCTCGTGCGGGc         <  1:1114059/93‑1 (MQ=255)
                                                                                       tATTCCGG‑GGGGCGTTGTGTTGCCGCTGTACTGCCGCGCTGACGGCTGATACTGCGAGCCCGGCACCCCCATATCCAGAGCCTCGTCCGGGCg        >  2:3973675/1‑93 (MQ=255)
                                                                                       tATTCCGG‑GGGGCGTTGTGTTGCCGCTGTACTGCCGCGCTGACGGCTGATACCGCGAGCCCGGTACCGCCATATCCAGCGCCTCGTGCGGGCg        <  1:2614812/93‑1 (MQ=255)
                                                                                       tATTCCGG‑GGGGCGTTGTGTTGCCGCTGTACTGCCGCGCTGACGGCTGATACCGCGAGCCCGGTACCGCCATATCCAGCGCCTCGTGCGGGCg        >  2:1511990/1‑93 (MQ=255)
                                                                                            c‑G‑GGGGCGTTGTGTTGCCGCTGTACTGCCGCGCTGACGGCTGATACCGCGAGCCCGGTCCCGCCATATCCAGCGCCTCGTGCGGGCGTTCAAg  <  1:4178231/93‑1 (MQ=255)
                                                                                             |                                                                                             
CTGAACGCCTTGCCTGCGCTCAGACTTACCCCTTTCACGCTCAGCTTTCCGCTGATATCCACTTTCCTGACCATCACCCCTTCATCGTATTCC‑G‑GGGGCGTTGTGTTGCCGCTGTACTGCCGCGCTGACGGCTGATACCGCGAGCCCGGTACCGCCATATCCAGCGCCTCGTGCGGGCGTTCAAG  >  NC_000913/280177‑280361

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑