Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 284,871 (G)6→7 coding (253/1383 nt) yagG → putative permease

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913284,8651.G96.9% 480.8 / 7.7 159coding (247/1383 nt)yagGputative permease
Reads supporting (aligned to +/- strand):  ref base . (1/4);  new base G (66/88);  total (67/92)
Fisher's exact test for biased strand distribution p-value = 3.98e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.67e-01

Only 100 of 190 total aligned reads displayed.

CCCGCGTGCTCGACGCCGTCACCGACCCGCTGATGGGGCTGCTGGTAGACCGCACCCGCACGCGGCACGGCCAGTTCCGCCCGTTCCTGCTGT‑GGGGGG‑CCATCCCGTTCGGCATCGTCTGCGTGCTGACCTTCTACACGCCGGACTTCTCCGCACAGGGCAAGATCATCTACGCCTGCG  >  NC_000913/284773‑284952
                                                                                             |                                                                                        
 ccGCGTGCTCGACGCCGTCACCGACCCGCTGATGGGGCTGCTGGTAGACCGCACCCGCACGCGGCACGGCCAGTTCCGCCCGTTCCTGCtgt‑g                                                                                         >  2:1270641/1‑93 (MQ=255)
 ccGCGTGCTCGACGCCGTCACCGACCCGCTGATGGGGCTGCTGGTAGACCGCACCCGCACGCGGCACGGCCAGTTCCGCCCGTTCCTACtgt‑g                                                                                         <  2:240909/93‑1 (MQ=255)
  cgcgTGCTCGACGCCGTCACCGACCCGCTGATGGGGCTGCTGGTAGACCGCACCCGCACGCGGCACGGCCAGTTCCGCCCGTTCCTGCTGT‑gg                                                                                        >  1:528965/1‑93 (MQ=255)
    cgTGCTCGACGCCGTCACCGACCCGCTGATGGGGCTGCTGGTAGACCGCACCCGCACGCGGCACGGCCAGTTCCGCCCGTTCCTGCTGT‑gggg                                                                                      <  2:734026/93‑1 (MQ=255)
    cgTGCTCGACGCCGTCACCGACCCGCTGATGGGGCTGCTGGTAGACCGCACCCGCACGCGGCACGGCCAGTTCCGCCCGTTCCTGCTGT‑gggg                                                                                      <  2:4332168/93‑1 (MQ=255)
    cgTGCTCGACGCCGTCACCGACCCGCTGATGGGGCTGCTGGTAGACCGCACCCGCACGCGGCACGGCCAGTTCCGCCCGTTCCTGCTGT‑gggg                                                                                      <  1:1812897/93‑1 (MQ=255)
     gTGCTCGACGCCGTCACCGACCCGCTGATGGGGCTGCTGGTAGACCGCACCCGCACGCGGCACGGCCAGTTCCGCCCGTTCCTGCTGT‑ggggg                                                                                     <  2:4624351/93‑1 (MQ=255)
      tGCTCGACGCCGTCACCGACCCGCTGATGGGGCTGCTGGTAGACCGCACCCGCACGCGGCACGGCCAGTTCCGCCCGTTCCTGCTGT‑gggggg                                                                                    <  1:1476979/93‑1 (MQ=255)
       gCTCGACGCCGTCACCGACCCGCTGATGGGGCTGCTGGTAGACCGCACCCGCACGCGGCACGGCCAGTTCCGCCCGTTCCTGCTGT‑ggggggg                                                                                   <  1:3139803/93‑2 (MQ=255)
        cTCGACGCCGTCACCGACCCGCTGATGGGGCTGCTGGTAGACCGCACCCGCACGCGGCACGGCCAGTTCCGCCCGTTCCTGCTGT‑gggggggc                                                                                  <  1:1078335/93‑3 (MQ=255)
         tCGACGCCGTCACCGACCCGCTGATGGGGCTGCTGGTAGACCGCACCCGCACGCGGCACGGCCAGTTCCGCCCGTTCCTGCTGT‑gggggggcc                                                                                 >  2:1823203/1‑90 (MQ=255)
         tCGACGCCGTCACCGACCCGCTGATGGGGCTGCTGGTAGACCGCACCCGCACGCGGCACGGCCAGTTCCGCCCGTTCCTGCTGT‑gggggggcc                                                                                 >  1:2687583/1‑90 (MQ=255)
            aCGCCGTCTCCGACCCGCTGATGGGGCTGCTGGTAGACCGCACCCGCACGCGGCACGGCCAGTTCCGCCCGTTCCTGCTGTGGGGGGG‑CCATc                                                                              <  2:2799470/93‑1 (MQ=255)
            aCGCCGTCACCGACCCGCTGATGGGGCTGCTGGTAGACCGCACCCGCACGCGGCACGGCCAGTTCCGCCCGTTCCTGCTGTGGGGGGG‑CCATc                                                                              >  2:2571626/1‑93 (MQ=255)
             cGCCGTCACCGACCCGCTGATGGGGCTGCTGGTAGACCGCACCCGCACGCGGCACGGCCAGTTCCGCCCGTTCCTGCTGTGGGGGGG‑CCATcc                                                                             <  1:792358/93‑1 (MQ=255)
                cGTCACCGACCCGCTGATGGGGCTGCTGGTAGACCGCACCCGCACGCGGCACGGCCAGTTCCGCCCGTTCCTGCTGTGGGGGGG‑CCATCCCGt                                                                          <  1:96509/93‑1 (MQ=255)
                cGTCACCGACCCGCTGATGGGGCTGCTGGTAGACCGCACCCGCACGCGGCACGGCCAGTTCCGCCCGTTCCTGCTGTGGGGGGG‑CCATCCCGt                                                                          <  1:4536749/93‑1 (MQ=255)
                 gTCACCGACCCGCTGATGGGGCTGCTGGTAGACCGCACCCGCACGCGGCACGGCCAGTTCCGCCCGTTCCTGCTGTGGGGGGG‑CCATCCCGtt                                                                         <  2:3802591/93‑1 (MQ=255)
                  tCACCGACCCGCTGATGGGGCTGCTGGTAGACCGCACCCGCACGCGGCACGGCCAGTTCCGCCCGTTCCTGCTGTGGGGGGG‑CCATCCCGTTc                                                                        >  2:1392939/1‑93 (MQ=255)
                  tCACCGACCCGCTGATGGGGCTGCTGGTAGACCGCACCCGCACGCGGCACGGCCAGTTCCGCCCGTTCCTGCTGTGGGGGGG‑CCATCCCGTTc                                                                        >  1:555980/1‑93 (MQ=255)
                  gcacccaccCGCTGATGGTGCTGCTGGTAGACCGCACCCGCACGCGGCACGGCCAGTTCCGCCCGTTCCTGCTGTGGGGGGG‑CCATCCCGTTc                                                                        <  1:1302227/92‑1 (MQ=255)
                   cACCGACCCGCTGATGGGGCTGCTGGTAGACCGCACCCGCACGCGGCACGGCCAGTTCCGCCCGTTCCTGCTGTGGGGGGG‑CCATCCCGTTCg                                                                       <  1:3394758/93‑1 (MQ=255)
                     ccGACCCGCTGATGGGGCTGCTGGTAGACCGCACCCGCACGCGGCACGGCCAGTTCCGCCCGTTCCTGCTGTGGGGGGG‑CCATCCCGTTCGGc                                                                     <  2:11548/93‑1 (MQ=255)
                     ccGACCCGCTGATGGGGCTGCTGGTAGACCGCACCCGCACGCGGCACGGCCAGTTCCGCCCGTTCCTGCTGTGGGGGGG‑CCATCCCGTTCGGc                                                                     <  1:2085380/93‑1 (MQ=255)
                       gACCCGCTGATGGGGCTGCTGGTAGACCGCACCCGCACGCGGCACGGCCAGTTCCGCCCGTTCCTGCTGTGGGGGGG‑CCATCCCGTTCGGCAt                                                                   <  1:118893/93‑1 (MQ=255)
                       gACCCGCTGATGGGGCTGCTGGTAGACCGCACCCGCACGCGGCACGGCCAGTTCCGCCCGTTCCTGCTGTGGGGGGG‑CCATCCCGTTCGGCAt                                                                   >  1:3359888/1‑93 (MQ=255)
                        gcccGCTGATGGGGCTGCTGGTAGACCGCACCCGCACGCGGCACGGCCAGTTCCGCCCGTTCCTGCTGTGGGGGGG‑CCATCCCGTTCGGCATc                                                                  <  2:3775126/92‑1 (MQ=255)
                         cccGCTGATGGGGCTGCTGGTAGACCGCACCCGCACGCGGCACGGCCAGTTCCGCCCGTTCATGCTGTGGGGGGG‑CCATCCCGTTCGGCATCg                                                                 <  1:1674832/93‑1 (MQ=255)
                           cGCTGATGGGGCTGCTGGTAGACCGCACCCGCACGCGGCACGGCCAGTTCCGCCCGTTCCTGCTGTGGGGGGG‑CCATCCCGTTCGGCATCGTc                                                               <  1:3686651/93‑1 (MQ=255)
                             cTGATGGGGCTGCTGGTAGACCGCACCCGCACGCGGCACGGCCAGTTCCGCCCGTTCCTGCTGTGGGGGGG‑CCATCCCGTTCGGCATCGTCTg                                                             >  2:3922664/1‑93 (MQ=255)
                             cTGATGGGGCTGCTGGTAGACCGCACCCGCACGCGGCACGGCCAGTTCCGCCCGTTCCTGCTGTGGGGGGG‑CCATCCCGTTCGGCATCGTCTg                                                             >  2:2101503/1‑93 (MQ=255)
                              tGATGGGGCTGCTGGTAGACCGCACCCGCACGCGGCACGGCCAGTTCCGCCCGTTCCTGCTGTGGGGGGG‑CCATCCCGTTCGGCATCGTCTGc                                                            >  2:971843/1‑93 (MQ=255)
                               gATGGGTCTGCTGGTAGACCGCACCCGCACGCGGCACGGCCAGTTCCGCCCGTTCCTGCTGTGGGGGGG‑CCATCCCGTTCGGCATCGTCTGCg                                                           <  2:30308/93‑1 (MQ=255)
                               gATGGGGCTGCTGGTAGACCGCACCCGCACGCGGCACGGCCAGTTCCGCCCGTTCCTGCTGTGGGGGGG‑CCATCCCGTTCGGCATCGTCTGCg                                                           >  1:881977/1‑93 (MQ=255)
                               gATGGGGCTGCTGGTAGACCGCACCCGCACGCGGCACGGCCAGTTCCGCCCGTTCCTGCTGTGGGGGGG‑CCATCCCGTTCGGCATCGTCTGCg                                                           <  2:4170818/93‑1 (MQ=255)
                                aTGGGGCTGCTGGTAGACCGCACCCGCACGCGGCACGGCCAGTTCCGCCCGTTCCTGCTGTGGGGGGG‑CCATCCCGTTCGGCATCGTCTGCGt                                                          >  1:4235150/1‑93 (MQ=255)
                                   gggCTGCTGGTAGACCGCACCCGCACGCGGCACGGCCAGTTCCGCCCGTTCCTGCTGTGGGGGGG‑CCATCCCGTTCGGCATCGTCTGCGTGCt                                                       <  1:293471/93‑1 (MQ=255)
                                    ggCTGCTGGTAGACCGCACCCGCACGCGGCACGGCCAGTTCCGCCCGTTCCTGCTGTGGGGGGG‑CCATCCCGTTCGGCATCGTCTGCGTGCTg                                                      >  1:1661250/1‑93 (MQ=255)
                                     gctgctGGTAGACCGCACCCGCACGGGGCACGGCCAGTTCCGCCCGTTCCTGCTGTGGGGGGG‑CCATCCCGTTCGGCATCGTCTGCGTGCTGa                                                     <  2:308633/93‑1 (MQ=255)
                                       tgctgGTAGACCGCACCCGCACGCGGCACGGCCAGTTCCGCCCGTTCCTGCTGTGGGGGGG‑CCATCCCGTTCGGCATCGTCTGCGTGCTGAcc                                                   >  1:3927853/1‑93 (MQ=255)
                                       tgctgGTAGACCGCACCCGCACGCGGCACGGCCAGTTCCGCCCGTTCCTGCTGT‑GGGGGG‑CCATCCCGTTCGGCATCGTCTGCGTGCTGACCt                                                  >  2:2160353/1‑93 (MQ=255)
                                           ggTAGACCGCACCCGCACGCGGCACGGCCAGTTCCGCCCGTTCCTGCTGTGGGGGGG‑CCATCCCGTTCGGCATCGTCTGCGTGCTGACCTTCt                                               >  1:1927328/1‑93 (MQ=255)
                                            gTAGACCGCACCCGCACGCGGCACGGCCAGTTCCGCCCGTTCCTGCTGTGGGGGGG‑CCATCCCGTTCGGCATCGTCTGCGTGCTGACCTTCTa                                              >  1:3582287/1‑93 (MQ=255)
                                              aGACCGCACCCGCACGCGGCACGGCCAGTTCCGCCCGTTCCTGCTGTGGGGGGG‑CCATCCCGTTCGGCATCGTCTGCGTGCTGACCTTCTaca                                            <  1:3541584/93‑1 (MQ=255)
                                               gACCGCACCCGCACGCGGCACGGCCAGTTCCGCCCGTTCCTGCTGTGGGGGGG‑CCATCCCGTTCGGCATCGTCTGCGTGCTGACCTTCTacac                                           <  2:4025385/93‑1 (MQ=255)
                                                aCCGCACCCGCACGCGGCACGGCCAGTTCCGCCCGTTCCTGCTGTGGGGGGG‑GCATCCCGTTCGGCATCGTCTGCGTGCTGACCGTCTACACg                                          >  1:844375/1‑93 (MQ=255)
                                                 ccgcacccgcacGCGGCACGGCCAGTTCCGCCCGTTCCTGCTGTGGGGGGG‑CCATCCCGTTCGGCATCGTCTGCGTGCTGACCTTCTACACGc                                         <  1:1503647/93‑1 (MQ=255)
                                                  cgcgcccgcagGCGGCACGGCCAGTTCCGCCCGTTCCTGCTGT‑GGGGGGTCCATCCCGTTCGGCATCGTCTGCGGGCTGACCTTCTACACGcc                                        <  2:335847/93‑1 (MQ=255)
                                                  cgcacccgcacGCGGCACGGCCAGTTCCGCCCGTTCCTGCTGTGGGGGGG‑CCATCCCGTTCGGCATCGTCTGCGTGCTGACCTTCTACACGcc                                        <  2:489409/93‑1 (MQ=255)
                                                    cacccgcacGCGGCACGGCCAGTTCCGCCCGTTCCTGCTGTGGGGGGG‑CCATCCCGTTCGGCATCGTCTGCGTGCTGACCTTCTACACGCCgg                                      >  2:4217596/1‑93 (MQ=255)
                                                     acccgcacGCGGCACGGCCAGTTCCGCCCGTTCCTGCTGTGGGGGGG‑CCATCCCGTTCGGCATCGTCTGCGTGCTGACCTTCTACACGCCGGa                                     >  1:3228185/1‑93 (MQ=255)
                                                      cccgcacGCGGCACGGCCAGTTCCGCCCGTTCCTGCTGTGGGGGGG‑CCATCCCGTTCGGCATCGTCTGCGTGCTGACCTTCTACACGCCGGAc                                    <  2:3530181/93‑1 (MQ=255)
                                                      cccgcacGCGGCACGGCCAGTTCCGCCCGTTCCTGCTGTGGGGGGG‑CCATCCCGTTCGGCATCGTCTGCGTGCTGACCTTCTACACGCCGGAc                                    >  2:2641739/1‑93 (MQ=255)
                                                      cccgcacGCGGCACGGCCAGTTCCGCCCGTTCCTGCTGTGGGGGGG‑CCATCCCGTTCGGCATCGTCTGCGTGCTGACCTTCTACACGCCGGAc                                    <  1:3511040/93‑1 (MQ=255)
                                                       ccgcacGCGGCACGGCCAGTTCCGCCCGTTCCTGCTGTGGGGGGG‑CCATCCCGTTCGGCATCGTCTGCGTGCTGACCTTCTACACGCCGGACt                                   <  1:2005300/93‑1 (MQ=255)
                                                       ccgcacGCGGCACGGCCAGTTCCGCCCGTTCCTGCTGTGGGGGGG‑CCATCCCGTTCGGCATCGTCTGCGTGCTGACCTTCTACACGCCGGACt                                   <  2:4529413/93‑1 (MQ=255)
                                                        cgcacGCGGCACGGCCAGTTCCGCCCGTTCCTGCTGTGGGGGGG‑CCATCCCGTTCGGCATCGTCTGCGTGCTGACCTTCTACACGCCGGACtt                                  >  2:1533244/1‑93 (MQ=255)
                                                         gcacGCGGCACGGCCAGTTCCGCCCGTTCCTGCTGTGGGGGGG‑CCATCCCGTTCGGCATCGTCTGCGTGCTGACCTTCTACACGCCGGACTtc                                 <  1:1775481/93‑1 (MQ=255)
                                                         gcacGCGGCACGGCCAGTTCCGCCCGTTCCTGCTGTGGGGGGG‑CCATCCCGTTCGGCATCGTCTGCGTGCTGACCTTCTACACGCCGGACTtc                                 <  2:2269088/93‑1 (MQ=255)
                                                          cgcgcgGCACGGCCAGTTCCGCCCGTTCCTGCTGTGGGGGGG‑CCATCCCGTTCGGCATCGTCTGCGTGCTGACCTTCTACACGCCGGACTtct                                <  2:4132536/91‑1 (MQ=255)
                                                           acGCGGCATGGCAAGTACCGCCCGTTCCTGCTGTGGGGGGG‑CCATCCCGTTCGGCATCGTCTGCGTGCTGACCTTCTACACGCCGGACTtctc                               <  2:3873378/93‑1 (MQ=255)
                                                           acGCGGCACGGCCAGTTCCGCCCGTTCCTGCTGTGGGGGGG‑CCATCCCGTTCGGCATCGTCTGCGTGCTGACCTTCTACACGCCGGACTtctc                               >  1:1517333/1‑93 (MQ=255)
                                                           acGCGGCACGGCCAGTTCCGCCCGTTCCTGCTGTGGGGGGG‑CCATCCCGTTCGGCATCGTCTGCGTGCTGACCTTCTACACGCCGGACTtctc                               >  1:1369718/1‑93 (MQ=255)
                                                            cgcgGCACGGCCAGTTCCGCCCGTGCCTGCTGTGGGGGGG‑CCATCCCGTTCGGCATCGTCTGCGTGCTGACCTTCTACACGCCGGACTTCTcc                              <  1:1162706/93‑1 (MQ=255)
                                                             gcgGCACGGCCAGTTCCGCCCGTTCCTACTGT‑GGGGGGACCATCCCGTTCGGCATCGTCTGCGTGCTGACCTTCTACACGCCGGACTTCTCCg                             <  2:4176502/93‑1 (MQ=255)
                                                                gCACGGCCAGTTCCGCCCGTTCCTGCTGTGGGGGGG‑CCATCCCGTTCGGCATCGTCTGCGTGCTGACCTTCTACACGCCGGACTTCTCCGcac                          <  1:4668111/93‑1 (MQ=255)
                                                                gCACGGCCAGTTCCGCCCGTTCCTGCTGTGGGGGGG‑CCATCCCGTTCGGCATCGTCTGCGTGCTGACCTTCTACACGCCGGACTTCTCCGcac                          <  1:4335243/93‑1 (MQ=255)
                                                                  aCGGCCAGTTCCGCCCGTTCCTGCTGTGGGGGGG‑CCATCCCGTTCGGCATCGTCTGCGTGCTGACCTTCTACACGCCGGACTTCTCCGCACAg                        >  2:1653993/1‑93 (MQ=255)
                                                                  aCGGCCAGTTCCGCCCGTTCCTGCTGTGGGGGGG‑CCATCCCGTTCGGCATCGTCTGCGTGCTGACCTTCTACACGCCGGACTTCTCCGCACAg                        >  2:1715016/1‑93 (MQ=255)
                                                                   cGGCCAGTTCCGCCCGTTCCTGCTGTGGGGGGG‑CCATCCCGTTCGGCATCGTCTGCGTGCTGACCTTCTACACGCCGGACTTCTCCGCACAgg                       <  2:1014045/93‑1 (MQ=255)
                                                                   cGGCCAGTTCCGCCCGTTCCTGCTGTGGGGGGG‑CCATCCCGTTCGGCATCGTCTGCGTGCTGACCTTCTACACGCCGGACTTCTCCGCACAgg                       <  2:2014521/93‑1 (MQ=255)
                                                                   cGGCCAGTTCCGCCCGTTCCTGCTGTGGGGGGG‑CCATCCCGTTCGGCATCGTCTGCGTGCTGACCTTCTACACGCCGGACTTCTCCGCACAgg                       <  2:3812260/93‑1 (MQ=255)
                                                                    ggCCAGTTCCGCCCGTTCCTGCTGTGGGGGGG‑CCATCCCGTTCGGCATCGTCTGCGTGCTGACCTTCTACACGCCGGACTTCTCCGCACAggg                      >  2:2729115/1‑93 (MQ=255)
                                                                       cAGTTCCGCCCGTTCCTGCTGT‑GGGGGGCCCATCCCGTTCGGCATCGTCTGCGTGCTGACCTTCTACACGCCGGACTTCTCCGCACAGGGCaa                   <  1:1262475/93‑1 (MQ=255)
                                                                        aGTTCCGCCCGTTCCTGCTGTGGGGGGG‑CCATCCCGTTCGGCATCGTCTGCGTGCTGACCTTCTACACGCCGGACTTCTCCGCACAGGGCAAg                  >  1:309112/1‑93 (MQ=255)
                                                                         tttCCGCCCGTTCCTGCTGTGGGGGGG‑CCATCCCGTTCGGCATCGTCTGCGTGCTGACCTTCTACACGCCGGACTTCTCCGCACAGGGCAAGa                 >  1:3754302/2‑93 (MQ=255)
                                                                         gTTCCGCCCGTTCCTGCTGTGGGGGGG‑CCATCCCGTTCGGCATCGTCTGCGTGCTGACCTTCTACACGCCGGACTTCTCCGCACAGGGCAAGa                 <  1:4296952/93‑1 (MQ=255)
                                                                         gTTCCGCCCGTTCCTGCTGTGGGGGGG‑CCATCCCGTTCGGCATCGTCTGCGTGCTGACCTTCTACACGCCGGACTTCTCCGCACAGGGCAAGa                 <  1:1409258/93‑1 (MQ=255)
                                                                         gTTCCGCCCGTTCCTGCTGTGGGGGGG‑CCATCCCGTTCGGCATCGTCTGCGTGCTGACCTTCTACACGCCGGACTTCTCCGCACACGGCAAGa                 >  2:4107579/1‑93 (MQ=255)
                                                                          ttCCGCCCGTTCCTGCTGTGGGGGGG‑CCATCCCGTTCGGCATCGTCTGCGTGCTGACCTTCTACACGCCGGACTTCTCCGCACAGGGCAAGat                <  2:2542329/93‑1 (MQ=255)
                                                                            ccGCCCGTTCCTGCTGTGGGGGGG‑CCATCCCGTTCGGCATCGTCTGCGTGCTGACCTTCTACACGCCGGACTTCTCCGCACAGGGCAAGatca              >  1:612274/1‑93 (MQ=255)
                                                                            ccGCCCGTTCCTGCTGTGGGGGGG‑CCATCCCGTTCGGCATCGTCTGCGTGCTGACCTTCTACACGCCGGACTTCTCCGCACAGGGCAAGatca              <  1:4448113/93‑1 (MQ=255)
                                                                             cGCCCGTTCCTGCTGTGGGGGGG‑CCATCCCGTTCGGCATCGTCTGCGTGCTGACCTTCTACACGCCGGACTTCTCCGCACAGGGCAAGatcat             <  1:4122663/93‑1 (MQ=255)
                                                                              gCCCGTTCCTGCTGTGGGGGGG‑CCATCCCGTTCGGCATCGTCTGCGTGCTGACCTTCTACACGCCGGACTTCTCCGCACAGGGCAAGctcatc            >  1:718781/1‑93 (MQ=255)
                                                                              gCCCGTTCCTGCTGTGGGGGGG‑CCATCCCGTTCGGCATCGTCTGCGTGCTGACCTTCTACACGCCGGACTTCTCCGCACAGGGCAAGatcatc            <  1:1018163/93‑1 (MQ=255)
                                                                              gCCCGTTCCTACTGTGGGGGGG‑CCATCCCGTTCGGCATCGTCTGCGTGCTGACCTTCTACACGCCGGACTTCTCCGCACAGGGCAAGatcatc            <  2:299421/93‑1 (MQ=255)
                                                                              ccccGTTCCTGCTGTGGGGGGG‑CCATCCCGTTCGGCATCGTCTGCGTGCTGACCTTCTACACGCCGGACTTCTCCGCACAGGGCAAGatcatc            <  1:1018166/92‑1 (MQ=255)
                                                                               cccGTTCCTGCTGTGGGGGGG‑CCATCCCGTTCGGCATCGTCTGCGTGCTGACCTTCTACACTCCGGACTTCTCCGCACAGGGCAAGATCATCt           >  2:1651671/1‑93 (MQ=255)
                                                                               cccGTTCCTGCTGTGGGGGGG‑CCATCCCGTTCGGCATCGTCTGCGTGCTGACCTTCTACACGCCGGACTTCTCCGCACAGGGCAAGATCATCt           >  2:848390/1‑93 (MQ=255)
                                                                                ccGTTCCTTCTGTGGGGGGG‑CCATCCCGTTCGGCATCGTCTGCGTGCTGACCTTCTACACGCCGGACTTCTCCGCACAGGGCAAGATCATCTa          <  2:1553262/93‑1 (MQ=255)
                                                                                ccGTTCCTGCTGTGGGGGGG‑CCATCCCGTTCGGCATCGTCTGCGTGCTGACCTTCTACACGCCGGACTTCTCCGCACAGGGCAAGATCATCTa          >  1:2050258/1‑93 (MQ=255)
                                                                                 cGTTCCTGCTGTGGGGGGG‑CCATCCCGTTCGGCATCGTCTGCGTGCTGACCTTCTACACGCCGGACTTCTCCGCACAGGGCAAGATCATCTAc         <  1:3079779/93‑1 (MQ=255)
                                                                                  gTTCCTGCTGTGGGGGGG‑CCATCCCGTTCGGCATCGTCTGCGTGCTGACCTTCTACACGCCGTACTTCTCCGCACAGGGCAAGATCATCTACg        <  2:1482029/93‑1 (MQ=255)
                                                                                  cttCCTGCTGTGGGGGGG‑CCATCCCGTTCGGCATCGTCTGCGTGCTGACCTTCTACACGCCGGACTTCTCCGCACAGGGCAAGATCATCTACg        <  1:434990/92‑1 (MQ=255)
                                                                                   ttCCTGCTGTGGGGGGG‑CCATCCCGTTCGGCATCGTCTGCGTGCTGACCTTCTACACGCCGGACTTCTCCGCACAGGGCAAGATCATCTACGc       >  1:2589148/1‑93 (MQ=255)
                                                                                   ttCCTGCTGTGGGGGGG‑CCATCCCGTTCGGCATCGTCTGCGTGCTGACCTTCTACACGCCGGACTTCTCCGCACAGGGCAAGATCATCTACGc       <  2:3586664/93‑1 (MQ=255)
                                                                                   gtCCTGCTGTGGGGGGG‑CCATCCCGTTCGGCATCGTCTGCGTGCTGACCTTCTACACGCCGGACTTCTCCGCACAGGGCAAGATCATCTACGc       <  1:3731285/92‑1 (MQ=255)
                                                                                     ccTGCTGT‑GGGGGGCCCATCCCGTTCGGCATCGTCTGCGTGCTGACCTTCTACACGCCGGACTTCTCCGCACAGGGCAAGATCATCTACGCCt     <  2:3616133/93‑1 (MQ=255)
                                                                                        gctgTGGGGGGG‑CCATCCCGTTCGGCATCGTCTGCGTGCTGACCTTCTACACGCCGGACTTCTCCGCACAGGGCAAGATCATCTACGCCTGCg  >  2:2312439/1‑93 (MQ=255)
                                                                                        gctgTGGGGGGG‑CCATCCCGTTCGGCATCGTCTGCGTGCTGACCTTCTACACGCCGGACTTCTCCGCACAGGGCAAGATCATCTACGCCTGCg  <  1:2786126/93‑1 (MQ=255)
                                                                                             |                                                                                        
CCCGCGTGCTCGACGCCGTCACCGACCCGCTGATGGGGCTGCTGGTAGACCGCACCCGCACGCGGCACGGCCAGTTCCGCCCGTTCCTGCTGT‑GGGGGG‑CCATCCCGTTCGGCATCGTCTGCGTGCTGACCTTCTACACGCCGGACTTCTCCGCACAGGGCAAGATCATCTACGCCTGCG  >  NC_000913/284773‑284952

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑