BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,732,563	(C)_8→9	coding (492/732 nt)	yfiH ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,732,555	1	.	C	94.9%	404.7 / 13.6	137	coding (500/732 nt)	yfiH	hypothetical protein
Reads supporting (aligned to +/- strand): ref base . (4/3); new base C (68/62); total (72/65)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.49e-01

Only 100 of 184 total aligned reads displayed.

CAAGCTGATAAATATCCGCCAGATACTTATCACCATGCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGA‑A‑CCT‑CCCC‑CCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATATTTTCCGGATTATCAGCAAAACAGGAAACCG  >  NC_000913/2732463‑2732642

cAAGCTGATAAATATCCGCCAGATACTTATCACCATGCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGA‑A‑CCt                                                                                           >  1:1991547/1‑93 (MQ=255)

 aaGCTGATAAATATCCGCCAGATACTTATCACCATGCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGA‑A‑CCT‑c                                                                                         >  2:2619105/1‑93 (MQ=255)

 aaGCTGATAAATATCCGCCAGATACTTATCACCATGCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGA‑A‑CCT‑c                                                                                         <  2:3314883/93‑1 (MQ=255)

 aaGCTGATAAATATCCGCCAGATACTTATCACCATGCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGA‑A‑CCT‑c                                                                                         >  1:2309483/1‑93 (MQ=255)

  aGCTGATAAATATCCGCCAGATACTTATCACCATGCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGA‑A‑CCT‑cc                                                                                        >  2:3882063/1‑93 (MQ=255)

   gCTGATAAATATCCGCCAGATACTTATCACCATGCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGA‑A‑CCT‑ccc                                                                                       <  2:204733/93‑1 (MQ=255)

     tGATAAATATCCGCCAGATACTTATCACCATGCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGA‑A‑CCT‑cccc‑c                                                                                    >  1:1157212/1‑93 (MQ=255)

      gATAAATATCCGCCAGATACTTATCACCATGCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGA‑A‑CCT‑cccc‑cc                                                                                   >  1:1524743/1‑93 (MQ=255)

      gATAAATATCCGCCAGATACTTATCACCATGCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGA‑A‑CCT‑cccc‑cc                                                                                   >  2:591345/1‑93 (MQ=255)

      gATAAATATCCGCCAGATACTTATCACCATGCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGA‑A‑CCT‑cccc‑cc                                                                                   >  1:3088928/1‑93 (MQ=255)

        tAAATATCCGCCAGATACTTATCACCATGCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGA‑A‑CCT‑cccc‑cccc                                                                                 >  2:3713505/1‑93 (MQ=255)

        tAAATATCCCCCAGATACTTATCACCATGCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGA‑A‑CCT‑cccc‑cccc                                                                                 >  1:361209/1‑93 (MQ=255)

            tatCCGCCAGATACTTATCACCATGCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGA‑A‑CCT‑cccc‑cccccact                                                                             >  1:4406700/1‑89 (MQ=255)

                 gCCAGATACTTATCACCATGCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGA‑A‑CCTCCCCC‑CCCCACTTCGaa                                                                         <  2:3488697/93‑1 (MQ=255)

                 gCCAGATACTTATCACCATGCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGA‑A‑CCTCCCCC‑CCCCACTTCGaa                                                                         <  1:4549725/93‑1 (MQ=255)

                  ccAGATACTTATCACCATGCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGA‑A‑CCTCCCCC‑CCCCACTTCGAAc                                                                        <  1:4336408/93‑1 (MQ=255)

                    aGATACTTATCACCATGCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGA‑A‑CCTCCCCC‑CCCCACTTCGAAcgc                                                                      >  1:4472564/1‑93 (MQ=255)

                      atccTTATCACCATGCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGA‑C‑CCTCCCCC‑CCCCACTTCGAAcgcgc                                                                    <  2:3121453/90‑1 (MQ=255)

                      aTACTTATCACCATGCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGA‑A‑CCTCCCCC‑CCCCACTTCGAAcgcgc                                                                    <  1:758391/93‑1 (MQ=255)

                       tACTTATCCCCATGCTGAATTAAAGCTTCACCTGCTTTAGCGGCTACTGCCATAAACGCCCCGCGA‑ACCCC‑CCCC‑CCCCACTTCGAAcgcgcg                                                                   <  1:3152889/93‑1 (MQ=255)

                       tACTTATCACCATGCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGA‑A‑CCTCCCCC‑CCCCACTTCGAAcgcgcg                                                                   >  1:1294884/1‑93 (MQ=255)

                        aCTTATCACCATGCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGA‑A‑CCTCCCCC‑CCCCACTTCGAACGCGCGt                                                                  >  1:4631660/1‑93 (MQ=255)

                        aCTTATCACCATGCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGA‑A‑CCTCCCCC‑CCCCACTTCGAACGCGCGt                                                                  <  1:1395675/93‑1 (MQ=255)

                          ttATCACCATGCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGA‑A‑CCTCCCCC‑CCCCACTTCGAACGCGCGTgg                                                                >  1:2071327/1‑93 (MQ=255)

                          ttATCACCATGCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGA‑A‑CCTCCCCC‑CCCCACTTCGAACGCGCGTgg                                                                <  2:4622568/93‑1 (MQ=255)

                            aTCACCATGCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGA‑A‑CCTCCCCC‑CCCCACTTCGAACGCGCGTGGAc                                                              <  2:4489230/93‑1 (MQ=255)

                            aTCACCATGCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGA‑A‑CCTCCCCC‑CCCCACTTCGAACGCGCGTGGAc                                                              >  1:3891199/1‑93 (MQ=255)

                             tCACCATGCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGA‑A‑CCTACCCC‑CCCCACTTCCACCGCGCGTCGAcc                                                             >  2:1300910/1‑93 (MQ=255)

                               aCCATGCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGA‑A‑CCTCCCCC‑CCCCACTTCGAACGCGCGTGGACCaa                                                           >  1:4361642/1‑93 (MQ=255)

                               aCCATGCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGA‑A‑CCTCCCCC‑CCCCACTTCGAACGCGCGTGGACCaa                                                           >  1:394382/1‑93 (MQ=255)

                               aCCATGCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGA‑A‑CCTCCCCC‑CCCCACTTCGAAACCGCGTGGACCaa                                                           >  1:293639/1‑93 (MQ=255)

                                ccATGCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGA‑A‑CCTCCCCC‑CCCCACTTCGCACGCGCGTGGACCAAt                                                          >  2:4116410/1‑93 (MQ=255)

                                ccATGCTGAATGAAAGCGCCACTTGCTTTAGCGTCTACTGCCATAAACGCCCCGCGA‑A‑CCTCCCCC‑CCCCACTTCGAACGCGCGTGGACCAAt                                                          <  2:1423478/93‑1 (MQ=255)

                                 cATGCTGAATGAAAGCTGCACTTGCTTTGGCGTCTACTGCCATAAACGCCTCGCGA‑A‑CCTCCCCC‑CCCCACTTCGAACGCGCGTGGACCAAtt                                                         <  1:4529813/93‑1 (MQ=255)

                                 cATGCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGA‑A‑CCTCCCCC‑CCCCACTTCGAACGCGCGTGGACCAAtt                                                         <  2:911983/93‑1 (MQ=255)

                                  aTGCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGA‑A‑CCTCCCCC‑CCCCACTTCGAACGGGCGTGGACAAAttc                                                        >  1:3268230/1‑92 (MQ=255)

                                   tGCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGA‑A‑CCTCCCCC‑CCCCACTTCGAACGCGCGTGGACCAATTGc                                                       <  1:3709715/93‑1 (MQ=255)

                                   tGCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGA‑A‑CCTCCCCC‑CCCCACTTCGAACGCGCGTGGACCAATTGc                                                       <  1:1316257/93‑1 (MQ=255)

                                    gCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGA‑A‑CCTCCCCC‑CCCCACTTCGAACGCGCGTGGACCAATTGcc                                                      >  2:4531530/1‑93 (MQ=255)

                                    gCTGAATGAAAGCTGCACTTGCTTTAGCCTCTACTGCCATAAACGCCTCGCGA‑G‑CCT‑CCCCGCCCCACTTCGAACGCGCGTGGACCAATTGcc                                                      <  2:3755203/93‑1 (MQ=255)

                                      tgaatgaaAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGA‑A‑CCTCCCCC‑CCCCACTTCGAACGCGCGTGGACCAATTGCCgg                                                    >  2:661041/1‑93 (MQ=255)

                                       gaatgaaAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGA‑A‑CCTCCCCC‑CCCCACTTCGAACGCGCGTGGACCAATTGCCGGc                                                   >  1:594924/1‑93 (MQ=255)

                                        aatgaaAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGA‑A‑CCTCCCCC‑CCCCACTTCGAACGCGCGTGGACCAATTGCCGGcc                                                  >  2:2163411/1‑93 (MQ=255)

                                          tgaaAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGA‑A‑CCTCCCCC‑CCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCt                                                <  2:4595847/93‑1 (MQ=255)

                                          tgaaAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGA‑A‑CCTCCCCC‑CCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCt                                                >  2:771022/1‑93 (MQ=255)

                                          tgaaAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGA‑A‑CCTCCCCC‑CCCCACCTCGAACGCGCGTGGACCAATTGCCCGCCCt                                                >  1:2854270/1‑93 (MQ=255)

                                          tgaaAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGA‑A‑CCT‑CCCC‑CCCCACTTTCAAACCGCGTGGGCCAAATGCCCGCCCTa                                               >  1:4369939/1‑93 (MQ=255)

                                           gaaAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGA‑A‑CCTCCCCC‑CCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTa                                               >  2:1244453/1‑93 (MQ=255)

                                             aaGCTGCACTTGCTTTAGCGTCTACTGCCATAACCGCCTCGCGA‑A‑CCTCCCCC‑CCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAAc                                             <  1:1739618/93‑1 (MQ=255)

                                             aaGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGA‑A‑CCTCCCCC‑CCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAAc                                             >  2:3377950/1‑93 (MQ=255)

                                             aaGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGA‑A‑CCTCCCCC‑CCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAAc                                             >  2:3808568/1‑93 (MQ=255)

                                             aaGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTAGCGA‑A‑CCTCCCCC‑CCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAAc                                             >  2:3080450/1‑93 (MQ=255)

                                              aGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGA‑A‑CCTCCCCC‑CCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAAcc                                            <  1:1170224/93‑1 (MQ=255)

                                              aGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGA‑A‑CCTCCCCC‑CCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAAcc                                            >  1:2626184/1‑93 (MQ=255)

                                              aGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGA‑A‑CCTCCCCC‑CCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAAcc                                            <  2:1903964/93‑1 (MQ=255)

                                               gCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGA‑A‑CCTCCCCC‑CCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCa                                           <  1:2620892/93‑1 (MQ=255)

                                                cTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGA‑ACCCC‑CCCC‑CCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAg                                          <  2:3203773/93‑1 (MQ=255)

                                                cTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGA‑A‑CCTCCCCC‑CCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAg                                          <  1:638937/93‑1 (MQ=255)

                                                cTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGA‑A‑CCTCCCCC‑CCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAg                                          <  2:4481631/93‑1 (MQ=255)

                                                cTGCACGTGCTTTAGCTTCTACTGCCATAAACGCCTCGCGA‑A‑CCTCCCCC‑CCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAg                                          <  1:3458415/93‑1 (MQ=255)

                                                  tcACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGA‑A‑CCTCCCCC‑CCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGc                                        <  2:2820305/92‑1 (MQ=255)

                                                  gCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGA‑A‑CCTCCCCC‑CCCCACCTCCAACCCGGGGGGACCAAATGGCGGCCCCAAACAggg                                        >  1:1057685/1‑92 (MQ=255)

                                                    aCTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGA‑A‑CCTCCCCC‑CCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCga                                      >  1:3367649/1‑93 (MQ=255)

                                                     cTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGA‑A‑CCTCCCCC‑CCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCgag                                     >  1:251638/1‑93 (MQ=255)

                                                      ttGCTTTAGCGTCTACTGCCATAAACGCCTCGCGA‑A‑CCTCCCCC‑CCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCgaga                                    >  1:1592389/1‑93 (MQ=255)

                                                      ttGCTTTAGCGTCTACTGCCATAAACGCCTCGCGA‑A‑CCTCCCCC‑CCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCgaga                                    >  2:1044861/1‑93 (MQ=255)

                                                      ttGCTTTAGCGTCTACTGCCATAAACGCCTCGCGA‑A‑CCTCCCCC‑CCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCgaga                                    >  1:3318962/1‑93 (MQ=255)

                                                      ttGCTTTAGCGTCTACTGCCATAAACGCCTCGCAA‑A‑CCTCCCCC‑CCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCgaga                                    >  1:2647014/1‑93 (MQ=255)

                                                       tGCTTTAGCGTCTCCTGCCATAAACGCCTCGCGA‑ACCCC‑CCCC‑CCCCACTACGAACGCGCGTGGGCCAATTGCCGGCCCTAACCAGGCGAGaa                                   <  1:4230694/93‑1 (MQ=255)

                                                       tGCTTTAGCGTCTACTGCCATAAACGCCTCGCGA‑A‑CCTCCCCC‑CCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGaa                                   <  2:2434179/93‑1 (MQ=255)

                                                       tGCTTTAGCGTCTACTGCCATAAACGCCTCGCGA‑A‑CCTCCCCC‑CCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGaa                                   <  2:1886240/93‑1 (MQ=255)

                                                        gCTTTAGCGTCTACTGCCATAAACGCCTCGCGA‑A‑CCT‑CCCC‑CCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAata                                 >  1:3694318/1‑93 (MQ=255)

                                                          tttAGCGTCTACTGCCATAAACGCCTCGCGA‑C‑CCTCCCCC‑CCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAatat                                <  2:1520564/93‑1 (MQ=255)

                                                          tttAGCGTCTACTGCCATAAACGCCTCGCGA‑A‑CCTCCCCC‑CCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAaaat                                >  1:2415982/1‑93 (MQ=255)

                                                              gCGTCTACTGCCATAAACGCCTCGCGA‑A‑CCTCCCCC‑CCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATATTTTc                            >  2:943063/1‑93 (MQ=255)

                                                              gCGTCTACTGCCATAAACGCCTCGCGA‑A‑CCTCCCCC‑CCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATATTTTc                            <  2:2111778/93‑1 (MQ=255)

                                                               cGTCTACTGCCATAAACGCCTCGCGA‑A‑CCTCCCCC‑CCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATATTTTcc                           <  2:4328085/93‑1 (MQ=255)

                                                                 tCTACTGCCATAAACGCCTCGCGA‑A‑CCTCCCCC‑CCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATATTTTCCgg                         >  1:2247322/1‑93 (MQ=255)

                                                                 tCTACTGCCATAAACGCCTCGCGA‑A‑CCTCCCCC‑CCCCACTTCCAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATATTTTcctg                         >  2:1733295/1‑91 (MQ=255)

                                                                     cTGCCATAAACGCCTCGCGA‑A‑CCTCCCCC‑CCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATATTTTCCGGATTa                     <  1:805212/93‑1 (MQ=255)

                                                                      tGCCATAAACGCCTCGCGA‑A‑CCTCCCCC‑CCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATATTTTCCGGATTAt                    >  2:2068674/1‑93 (MQ=255)

                                                                      tGCCATAAACGCCTCGCGA‑A‑CCTCCCCC‑CCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATATTTTCCGGATTAt                    >  2:606922/1‑93 (MQ=255)

                                                                       gCCATAAACGCCTCGCGATC‑CCC‑CCCC‑CCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATATTTTCCGGATTATc                   <  1:4488871/93‑1 (MQ=255)

                                                                        ccATAAACGCCTCGCGA‑C‑CCTCCCCC‑CCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATATTTTCCGGATTATCa                  <  1:2104877/93‑1 (MQ=255)

                                                                        ccATAAACGCCTCGCGA‑C‑CCTCCCCC‑CCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATATTTTCCGGATTATCa                  <  2:5484/93‑1 (MQ=255)

                                                                         cATAAACGCCTCGCGA‑A‑CCTCCCCC‑CCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATATTTTCCGGATTATCAg                 >  2:4063764/1‑93 (MQ=255)

                                                                         cATAAACGCCTCGCGA‑A‑CCTCCCCC‑CCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATATTTTCCGGATTATCAg                 <  1:969813/93‑1 (MQ=255)

                                                                          aTAAACGCCTCGCGA‑A‑CCTCCCCC‑CCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATATTTTCCGGATTATCAga                >  2:1355940/1‑92 (MQ=255)

                                                                           tAAACGCCTCGCGA‑A‑CCTCCCCC‑CCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTCACCAGGCGAGAATATTTTTCGTATTATCAGCa               >  1:4281383/1‑93 (MQ=255)

                                                                           tAAACGCCTCGCGA‑A‑CCTCCCCC‑CCCCACTTCGAACGCGCGGGGACCAATTGCCGGCCCTAACCAGCCGAGAATATTTTCGGGATTATCAGCa               >  2:1658008/1‑93 (MQ=255)

                                                                            aaaCGCCTCGCGA‑A‑CCTCCCCC‑CCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATATTTTCCGGATTATCAGCaa              <  2:2979560/93‑1 (MQ=255)

                                                                             aaCGCCTCGCGA‑A‑CCTCCCCC‑CCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATATTTTCCGGATTATCAGCaaa             >  2:3239337/1‑93 (MQ=255)

                                                                                 ccTCGCGACC‑CCC‑CCCC‑CCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATATTTTCCGGATTATCAGCAAAACAg         <  1:933363/93‑1 (MQ=255)

                                                                                 ccTCGCGA‑G‑CCTCCCCC‑CCCCGCTGCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATATTTTCCGGATTATCAGCAAAACAg         <  2:2371602/93‑1 (MQ=255)

                                                                                  cTCGCGA‑A‑CCTCCCCC‑CCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATATTTTCCGGATTATCAGCAAAACAgg        <  1:3721285/93‑1 (MQ=255)

                                                                                   tCGCGA‑A‑CCTCCCCC‑CCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATATTTTCCGGATTATCAGCaa              >  1:2989449/1‑86 (MQ=255)

                                                                                   tCGCGA‑A‑CCTCCCCC‑CCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATATTTTCCGGATTATCAGCAAAACAGGa       >  2:1681830/1‑93 (MQ=255)

                                                                                       gA‑A‑CCTCCCCC‑CCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATATTTTCCGGATTATCAGCAAAACAGGAAAcc   <  1:1243531/93‑1 (MQ=255)

                                                                                        a‑a‑CCTCCCCC‑CCCCACTTCGACCGCGCCTGTACCAATTTACGGCCCTAACCAGGCGATAATATTTTTCGCATTATCCGCAAAACAGGCAAcct  >  2:730819/1‑92 (MQ=255)

                                                                                        a‑a‑CCTCCCCC‑CCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATATTTTCCGGATTATTAGCAAAACAGGAAACCg  >  2:1445621/1‑93 (MQ=255)

CAAGCTGATAAATATCCGCCAGATACTTATCACCATGCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGA‑A‑CCT‑CCCC‑CCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATATTTTCCGGATTATCAGCAAAACAGGAAACCG  >  NC_000913/2732463‑2732642

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 16 ≤` `ATCG/ATCG` `< 24 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`