BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,825,077	T→C	C221C (TGT→TGC)	srlE →	PTS system glucitol/sorbitol‑specific enzyme IIBC component

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,825,077	0	T	C	100.0%	488.8 / NA	154	C221C (TGT→TGC)	srlE	PTS system glucitol/sorbitol‑specific enzyme IIBC component
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (63/91); total (63/91)

Only 100 of 173 total aligned reads displayed.

TATGGCTTCTGGCCTTGGTGACTGGATTGCCCACGGTCTTGCTCCGCTGGCGAGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTGTTCCTTCCCACTGCTTTCACCTTTCTTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTGGTCTCGG  >  NC_000913/2824987‑2825166

 aTGGCTTCTGGCCTTGGTGACTGGATTGCCCACGGTCTTGCTCCGCTGGCGAGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTGctcc                                                                                        <  1:3997970/93‑1 (MQ=255)

  tGGCTTCTGGCCTTGGTGACTGGATTGCCCACGGTCTTGCTCCGCTGGCGAGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTGctcct                                                                                       >  2:195719/1‑93 (MQ=255)

  tGGCTTCTGGCCTTGGTGACTGGATTGCCCACGGTCTTGCTCCGCTGGCGAGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTGctcct                                                                                       <  2:1842265/93‑1 (MQ=255)

  tGGCTTCTGGCCTTGGTGACTGGATTGCCCACGGTCTTGCTCCGCTGGCGAGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTGctcct                                                                                       <  1:239827/93‑1 (MQ=255)

   ggCTTCTGGCCTTGGTGACTGGATTGCCCACGGTCTTGCTCCGCTGGCGAGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTGctcctt                                                                                      >  1:3266012/1‑93 (MQ=255)

    gCTTCTGGCCTTGGTGACTGGATTGCCCACGGTCTTGCTCCGCTGGCGAGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTGctccttc                                                                                     >  2:3046977/1‑93 (MQ=255)

    gCTTCTGGCCTTGGTGACTGGATTGCCCACGGTCTTGCTCCGCTGGCGAGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTGctccttc                                                                                     <  2:1065084/93‑1 (MQ=255)

      ttCTGGCCTTGGTGACTGGATTGCCCACGGTCTTGCTCCGCTTGCGAGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTGCTCCTTccc                                                                                   >  2:464551/1‑93 (MQ=255)

       tCTGGCCTTGGTGACTGGATTGCCCACGGTCTTGCTCCGCTGGCGAGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTGCTCCTTCCCa                                                                                  >  1:730131/1‑93 (MQ=255)

        cTGGCCTTGGTGACTGGATTGCCCACGGTCTTGCTCCGCTGGCGAGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTGCTCCTTCCCAc                                                                                 >  2:3659233/1‑93 (MQ=255)

          ggCCTTGGTGACTGGATTGCCCACGGTCTTGCTCCGCTGGCGAGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTGCTCCTTCCCACTg                                                                               >  1:1956073/1‑93 (MQ=255)

           gCCTTGGTGACTGGATTGCCCACGGTCTTGCTCCGCTGGCGAGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTGCTCCTTCCCACTGc                                                                              >  1:3938910/1‑93 (MQ=255)

              ttGGTGACTGGATTGCCCACGGTCTTGCTCCGCTGGCGAGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCttt                                                                           >  1:2726060/1‑93 (MQ=255)

              ttGGTGACTGGATTGCCCACGGTCTTGCTCCGCTGGCGAGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCttt                                                                           >  1:1832703/1‑93 (MQ=255)

               tGGTGACTGGATTGCCCACGGTCTTGCTCCGCTGGCGAGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTc                                                                          <  2:3376505/93‑1 (MQ=255)

                ggTGACTGGATTGCCCACGGTCTTGCTCCGCTGGCGAGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCa                                                                         <  1:3183183/93‑1 (MQ=255)

                 gTGACTGGATTGCCCACGGTCTTGCTCCGCTGGCGAGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCAc                                                                        <  2:3392589/93‑1 (MQ=255)

                 gTGACTGGATTGCCCACGGTCTTGCTCCGCTGGCGAGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCAc                                                                        <  1:3481640/93‑1 (MQ=255)

                 gTGACTGGATTGCCCACGGTCTTGCTCCGCTGGCGAGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCAc                                                                        >  1:637609/1‑93 (MQ=255)

                  tGACTGGATTGCCCACGGTCTTGCTCCGCTGGCGAGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCAcc                                                                       >  2:1262164/1‑93 (MQ=255)

                   taCTGGATTGCCCACGGTCTTGCTCCGCTGGCGACCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCTCCt                                                                      <  2:2377864/92‑1 (MQ=255)

                   gACTGGATTGCCCACGGTCTTGCTCCGCTGGCGAGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCt                                                                      <  2:1909326/93‑1 (MQ=255)

                   gACTGGATTGCCCACGGTCTTGCTCCGCTGGCGAGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCt                                                                      >  1:1460213/1‑93 (MQ=255)

                   gACTGGATTGCCCACGGTCTTGCTCCGCTGGCGAGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCt                                                                      >  2:3823468/1‑93 (MQ=255)

                    aCTGGATTGCCCACGGTCTTGCTCCGCTGGCGAGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCtt                                                                     <  2:1601725/93‑1 (MQ=255)

                    aCTGGATTGCCCACGGTCTTGCTCCGCTGGCGAGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCtt                                                                     >  2:59495/1‑93 (MQ=255)

                     cTGGATTGCCCACGGTCTTGCTCCGCTGGCGAGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCttt                                                                    >  1:4594026/1‑93 (MQ=255)

                       ggATTGCCCACGGTCTTGCTCGGCTGGCGAGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTttcc                                                                  <  2:874237/93‑2 (MQ=255)

                       ggATTGCCCACGGTCTTGCTCCGCTGGCGAGCCATCCCCTGGGTCTGGTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTttcc                                                                  >  1:4613367/1‑92 (MQ=255)

                       ggATTGCCCACGGTCTTGCTCCGCTGGCGAGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTttcc                                                                  >  2:3450630/1‑92 (MQ=255)

                         aTTCCCCACGGTCTTGCTCCGCTGGCGAGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTGCTCCTTCCCCCTGCTTTCACCTttcctc                                                                <  2:1521475/93‑1 (MQ=255)

                          ttGCCCACGGTCTTGCTCCGCTGGCGAGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCg                                                               <  2:4550054/93‑1 (MQ=255)

                              ccACGGTCTTGCTCCGCTGGCGAGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGccc                                                           >  2:410737/1‑93 (MQ=255)

                               cACGGTCTTGCTCCGCTGGCGAGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCa                                                          <  1:1020367/93‑1 (MQ=255)

                                aCGGTCTTGCTCCGCTGGCGAGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAg                                                         <  2:2676810/93‑1 (MQ=255)

                                aCGGTCTTGCTCCGCTGGCGAGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAg                                                         <  2:312896/93‑1 (MQ=255)

                                aCGGTCTTGCTCCGCTGGCGAGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAg                                                         >  1:2264167/1‑93 (MQ=255)

                                 cGGTCTTGCTCCGCTGGCGAGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAgg                                                        <  1:3109711/93‑1 (MQ=255)

                                  ggTCTTGCTCCGCTGGCGAGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGgc                                                       >  2:2881678/1‑93 (MQ=255)

                                  ggTCTTGCTCCGCTGGCGAGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGgc                                                       <  1:3600211/93‑1 (MQ=255)

                                  ggTCTTGCTCCGCTGGCGAGCCATCCACTGGGTCTGCTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGgc                                                       <  2:3209973/93‑1 (MQ=255)

                                   gTCTTGCTCCGCTGGCGAGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGgcg                                                      >  2:2620383/1‑93 (MQ=255)

                                   gTCTTGCTCCGCTGGCGAGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGgcg                                                      >  2:1572413/1‑93 (MQ=255)

                                    tCTTGCTCCGCTGGCGAGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGgcgc                                                     >  2:2924268/1‑93 (MQ=255)

                                    tCTTGCTCCGCTGGCGAGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGgcgc                                                     <  1:1895180/93‑1 (MQ=255)

                                     cTTGCTCCGCTGGCGAGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCa                                                    >  1:3674835/1‑93 (MQ=255)

                                      ttGCTCCGCTGGCGAGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAg                                                   <  2:4187752/93‑1 (MQ=255)

                                       tGCTCCGCTGGCGAGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGt                                                  <  1:2509088/93‑1 (MQ=255)

                                        gCTCCGCTGGCGAGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGtt                                                 >  2:1126575/1‑93 (MQ=255)

                                         cTCCGCTGGCGAGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTa                                                <  2:2052130/93‑1 (MQ=255)

                                           ccGCTGGCGAGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATc                                              <  1:915152/93‑1 (MQ=255)

                                           ccGCTGGCGAGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATc                                              <  1:194700/93‑1 (MQ=255)

                                              cTGGCGAGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGca                                           <  1:1365619/93‑1 (MQ=255)

                                              cTGGCGAGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGca                                           <  2:2817928/93‑1 (MQ=255)

                                              cTGGCGAGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGca                                           >  1:3363925/1‑93 (MQ=255)

                                               tGGCGAGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGcac                                          <  2:1778430/93‑1 (MQ=255)

                                               tGGCGAGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGcac                                          >  1:1645657/1‑93 (MQ=255)

                                                ggCGAGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGcaca                                         <  2:1673568/93‑1 (MQ=255)

                                                ggCGAGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGcaca                                         <  2:4244127/93‑1 (MQ=255)

                                                ggCGAGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGcaca                                         <  1:1567079/93‑1 (MQ=255)

                                                 gCGAGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAg                                        <  2:2715341/93‑1 (MQ=255)

                                                  ggAGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAgg                                       <  2:2829003/92‑1 (MQ=255)

                                                   gAGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGt                                      <  1:3886634/93‑1 (MQ=255)

                                                     gCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTa                                    <  1:283889/93‑1 (MQ=255)

                                                     gCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTa                                    >  1:3576525/1‑93 (MQ=255)

                                                      ccATCCACTGGGTCTGGTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTAt                                   <  1:245804/93‑1 (MQ=255)

                                                      ccATCCACTGGGTCTGGTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTAt                                   <  1:4362851/93‑1 (MQ=255)

                                                      ccATCCACTGGGTCTGGTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTAt                                   >  1:1320972/1‑93 (MQ=255)

                                                         tCCACTGGGTCTGGTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCgg                                >  1:2118953/1‑93 (MQ=255)

                                                          ccACTGGGTCTGGTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGc                               <  1:4236326/93‑1 (MQ=255)

                                                           cACTGGGTCTGGTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCg                              <  1:4668602/93‑1 (MQ=255)

                                                             cTGGGTCTGGTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCCGTTATCGCACAGGTTATCGGCGTa                            >  2:2797128/1‑93 (MQ=255)

                                                             cTGGGTCTGGTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTa                            <  1:1679047/93‑1 (MQ=255)

                                                              tGGGTCTGGTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTat                           <  2:4532586/93‑1 (MQ=255)

                                                              tGGGTCTGGTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTat                           <  1:1071082/93‑1 (MQ=255)

                                                              tGGGTCTGGTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTat                           <  1:1246557/93‑1 (MQ=255)

                                                              tGGGTCTGGTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTat                           <  1:3359215/93‑1 (MQ=255)

                                                                 gTCTGGTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTattga                        <  2:4300538/93‑1 (MQ=255)

                                                                  tCTGGTCATGCTGGCTCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTattgat                       <  2:764446/93‑1 (MQ=255)

                                                                    tGGTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTattgattg                     <  2:3962641/93‑1 (MQ=255)

                                                                     ggTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTgg                    <  1:2999005/93‑1 (MQ=255)

                                                                     ggTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTgg                    >  2:1139214/1‑93 (MQ=255)

                                                                     ggTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTgg                    <  1:4652816/93‑1 (MQ=255)

                                                                         aTGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTCATTTGCGTg                >  1:3977720/1‑93 (MQ=255)

                                                                          tGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGc               >  2:4065372/1‑93 (MQ=255)

                                                                             tGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGa            <  2:1323683/93‑1 (MQ=255)

                                                                              ggCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGAt           <  2:2118953/93‑1 (MQ=255)

                                                                               gcgcTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGAtt          <  1:4293562/93‑1 (MQ=255)

                                                                                cgcTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTg         <  2:459547/93‑1 (MQ=255)

                                                                                  cTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTGGt       >  2:2531901/1‑93 (MQ=255)

                                                                                  cTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTGGt       <  2:4443043/93‑1 (MQ=255)

                                                                                  cTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTGGt       >  2:1167444/1‑93 (MQ=255)

                                                                                   tCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCTCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTGGtc      <  2:1868329/93‑1 (MQ=255)

                                                                                   tCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTGGtc      <  2:3532691/93‑1 (MQ=255)

                                                                                    cATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTGGtct     <  2:636732/93‑1 (MQ=255)

                                                                                     aTCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTGGtctc    <  1:3721079/93‑1 (MQ=255)

                                                                                     aTCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTGGtctc    <  1:2190477/93‑1 (MQ=255)

                                                                                      tCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTGGTCTCg   >  2:3641885/1‑93 (MQ=255)

                                                                                      tCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTGGTCTCg   >  2:508495/1‑93 (MQ=255)

                                                                                       cTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTGGTCTCgg  <  1:3947094/93‑1 (MQ=255)

TATGGCTTCTGGCCTTGGTGACTGGATTGCCCACGGTCTTGCTCCGCTGGCGAGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTGTTCCTTCCCACTGCTTTCACCTTTCTTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTGGTCTCGG  >  NC_000913/2824987‑2825166

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`