BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,825,102	T→C	F230L (TTC→CTC)	srlE →	PTS system glucitol/sorbitol‑specific enzyme IIBC component

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,825,102	0	T	C	100.0%	580.8 / NA	182	F230L (TTC→CTC)	srlE	PTS system glucitol/sorbitol‑specific enzyme IIBC component
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (75/107); total (75/107)

Only 100 of 186 total aligned reads displayed.

ATTGCCCACGGTCTTGCTCCGCTGGCGAGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTGTTCCTTCCCACTGCTTTCACCTTTCTTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTGGTCTCGGCAATATTCCGCCGCATCTGGCTTT  >  NC_000913/2825012‑2825190

aTTGCCCACGGTCTTGCTCCGCTGGCGAGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTttcctc                                                                                        <  2:838805/93‑1 (MQ=255)

aTTCCCCACGGTCTTGCTCCGCTGGCGAGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTGCTCCTTCCCCCTGCTTTCACCTttcctc                                                                                        <  2:1521475/93‑1 (MQ=255)

     ccACGGTCTTGCTCCGCTGGCGAGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGccc                                                                                   >  2:1245127/1‑93 (MQ=255)

     ccACGGTCTTGCTCCGCTGGCGAGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGccc                                                                                   <  2:1865332/93‑1 (MQ=255)

     ccACGGTCTTGCTCCGCTGGCGAGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGccc                                                                                   >  2:410737/1‑93 (MQ=255)

      cACGGTCTTGCTCCGCTGGCGAGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCa                                                                                  <  1:1020367/93‑1 (MQ=255)

       aCGGTCTTGCTCCGCTGGCGAGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAg                                                                                 <  2:312896/93‑1 (MQ=255)

         ggTCTTGCTCCGCTGGCGAGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGgc                                                                               <  1:3687125/93‑1 (MQ=255)

         ggTCTTGCTCCGCTGGCGAGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGgc                                                                               <  1:3600211/93‑1 (MQ=255)

         ggTCTTGCTCCGCTGGCGAGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGgc                                                                               >  2:2881678/1‑93 (MQ=255)

         ggTCTTGCTCCGCTGGCGAGCCATCCACTGGGTCTGCTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGgc                                                                               <  2:3209973/93‑1 (MQ=255)

          gTCTTGCTCCGCTGGCGAGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGgcg                                                                              >  2:1167233/1‑93 (MQ=255)

          gTCTTGCTCCGCTGGCGAGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGgcg                                                                              >  2:1572413/1‑93 (MQ=255)

           tCTTGCTCCGCTGGCGAGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGgcgc                                                                             >  1:3730404/1‑93 (MQ=255)

           tCTTGCTCCGCTGGCGAGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGgcgc                                                                             <  1:1895180/93‑1 (MQ=255)

            cTTGCTCCGCTGGCGAGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCa                                                                            >  1:3674835/1‑93 (MQ=255)

             ttGCTCCGCTGGCGAGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAg                                                                           >  2:4453779/1‑93 (MQ=255)

             ttGCTCCGCTGGCGAGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAg                                                                           <  2:4187752/93‑1 (MQ=255)

                cTCCGCTGGCGAGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTa                                                                        <  1:3230123/93‑1 (MQ=255)

                  ccGCTGGCGAGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATc                                                                      <  1:194700/93‑1 (MQ=255)

                     cTGGCGAGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGca                                                                   <  2:2817928/93‑1 (MQ=255)

                     cTGGCGAGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGca                                                                   >  1:4304751/1‑93 (MQ=255)

                     cTGGCGAGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGca                                                                   >  1:3363925/1‑93 (MQ=255)

                      tGGCGAGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGcac                                                                  >  1:1645657/1‑93 (MQ=255)

                       ggCGAGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGcaca                                                                 <  1:1567079/93‑1 (MQ=255)

                       ggCGAGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGcaca                                                                 >  2:1912393/1‑93 (MQ=255)

                        gCGAGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAg                                                                <  2:2715341/93‑1 (MQ=255)

                            gCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTa                                                            >  1:3576525/1‑93 (MQ=255)

                            gCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTa                                                            <  1:283889/93‑1 (MQ=255)

                            gCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTa                                                            <  2:2922910/93‑1 (MQ=255)

                            gCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTa                                                            >  1:985952/1‑93 (MQ=255)

                             ccATCCACTGGGTCTGGTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTAt                                                           <  1:4362851/93‑1 (MQ=255)

                             ccATCCACTGGGTCTGGTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTAt                                                           >  1:1320972/1‑93 (MQ=255)

                                tCCACTGGGTCTGGTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCgg                                                        >  1:2118953/1‑93 (MQ=255)

                                tCCACTGGGTCTGGTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCgg                                                        >  2:182121/1‑93 (MQ=255)

                                tCCACTGGGTCTGGTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCgg                                                        <  2:347735/93‑1 (MQ=255)

                                 ccACTGGGTCTGGTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGc                                                       <  1:4236326/93‑1 (MQ=255)

                                  cACTGGGTCTGGTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCg                                                      <  1:4668602/93‑1 (MQ=255)

                                    cTGGGTCTGGTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCCGTTATCGCACAGGTTATCGGCGTa                                                    >  2:2797128/1‑93 (MQ=255)

                                    cTGGGTCTGGTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTa                                                    >  2:2961566/1‑93 (MQ=255)

                                     tGGGTCTGGTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTat                                                   <  2:4532586/93‑1 (MQ=255)

                                     tGGGTCTGGTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTat                                                   <  1:1071082/93‑1 (MQ=255)

                                     tGGGTCTGGTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTat                                                   <  1:1246557/93‑1 (MQ=255)

                                      gggTCTGGTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTatt                                                  <  1:782743/93‑1 (MQ=255)

                                       ggTCTGGTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTattg                                                 >  1:3062812/1‑93 (MQ=255)

                                        gTCTGGTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTattga                                                <  2:4300538/93‑1 (MQ=255)

                                         tCTGGTCATGCTGGCTCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTattgat                                               <  2:764446/93‑1 (MQ=255)

                                         tCTGGTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTattgat                                               <  1:1715154/93‑1 (MQ=255)

                                          cTGGTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTattgatt                                              <  2:2006947/93‑1 (MQ=255)

                                           tGGTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGTGTattgattg                                             >  2:1768785/1‑93 (MQ=255)

                                           tGGTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTattgattg                                             <  2:3962641/93‑1 (MQ=255)

                                           tGGTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTattgattg                                             >  1:3901799/1‑93 (MQ=255)

                                            ggTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTgg                                            <  1:2999005/93‑1 (MQ=255)

                                                aTGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTCATTTGCGTg                                        >  1:3977720/1‑93 (MQ=255)

                                                 tGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGc                                       <  2:4533894/93‑1 (MQ=255)

                                                 tGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGc                                       >  2:4065372/1‑93 (MQ=255)

                                                  gCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGTTTGGCGTGCa                                      <  2:2868036/93‑1 (MQ=255)

                                                     ggCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGAt                                   >  1:1401770/1‑93 (MQ=255)

                                                     ggCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGAt                                   <  2:2118953/93‑1 (MQ=255)

                                                      gcgcTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGAtt                                  <  2:1504277/93‑1 (MQ=255)

                                                       cgcTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTg                                 <  2:459547/93‑1 (MQ=255)

                                                         cTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTGGt                               >  2:2531901/1‑93 (MQ=255)

                                                          tCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTGGtc                              <  2:3532691/93‑1 (MQ=255)

                                                          tCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTGGtc                              >  1:2502928/1‑93 (MQ=255)

                                                            aTCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTGGtctc                            <  1:2190477/93‑1 (MQ=255)

                                                             tCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTGGTCTCg                           >  2:508495/1‑93 (MQ=255)

                                                             tCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTGGTCTCg                           >  2:3641885/1‑93 (MQ=255)

                                                             tCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTGGTCTCg                           <  2:4674301/93‑1 (MQ=255)

                                                               tgctccttccCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTGGTCTCGGc                         >  1:4194412/4‑93 (MQ=255)

                                                               tgctccttccCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTGGTCTCGGc                         >  2:1040589/4‑93 (MQ=255)

                                                               tgctccttccCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTGGTCTCGGc                         >  2:1130002/4‑93 (MQ=255)

                                                               tgctccttccCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTGGTCTCGGc                         <  1:1800672/90‑1 (MQ=255)

                                                               tgctccttccCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTGGTCTCGGc                         >  1:4019098/4‑93 (MQ=255)

                                                               tgctccttccCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTGGTCTCGGc                         <  2:3458938/90‑1 (MQ=255)

                                                               tgctccttccCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTGGTCTCGGc                         >  2:655726/4‑93 (MQ=255)

                                                                gctccttccCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTGGTCTCGGCa                        <  2:228286/91‑1 (MQ=255)

                                                                 ctccttccCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTTGCCTCGGCac                       >  2:4238004/2‑92 (MQ=255)

                                                                  tccttccCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTGGTCTCGGCAat                      <  2:1658839/93‑1 (MQ=255)

                                                                  tccttccCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTGGTCTCGGCAat                      <  2:2655093/93‑1 (MQ=255)

                                                                   ccttccCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTGGTCTCGGCAata                     <  1:2927766/93‑1 (MQ=255)

                                                                     ttccCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTGGTCTCGGCAATAtt                   <  2:3753132/93‑1 (MQ=255)

                                                                          aCTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTGGTCTCGGCAATATTccgcc              >  1:2283433/1‑93 (MQ=255)

                                                                          aCTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTGGTCTCGGCAATATTccgcc              >  2:4492356/1‑93 (MQ=255)

                                                                          aCTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTGGTCTCGGCAATATTccgcc              >  1:1725421/1‑93 (MQ=255)

                                                                          aCTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTGGTCTCGGCAATATTcagcc              >  2:2561462/1‑93 (MQ=255)

                                                                             gCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTGGTCTCGGCAATATTCCGCCGCa           <  2:2212144/93‑1 (MQ=255)

                                                                             gCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTGGTCTCGGCAATATTCCGCCGCa           >  1:2396269/1‑93 (MQ=255)

                                                                               tttCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTGGTCTCGGCAATATTCCGCCGCATc         >  1:426764/1‑93 (MQ=255)

                                                                               tttCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTGGTCTCGGCAATATTCCGCCGCATc         <  2:3725742/93‑1 (MQ=255)

                                                                               tttCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTGGTCTCGGCAATATTCCGCCGCATc         <  2:2697825/93‑1 (MQ=255)

                                                                               tttCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTGGTCTCGGCAATATTCCGCCGCATc         <  1:4550633/93‑1 (MQ=255)

                                                                                ttCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTGGTCTCGGCAATATTCCGCCGCATCt        >  2:121859/1‑93 (MQ=255)

                                                                                 tCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTGGTCTCGGCAATATTCCGCCGCATCTg       <  1:4641111/93‑1 (MQ=255)

                                                                                 tCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTGGTCTCGGCAATATTCCGCCGCATCTg       <  1:731191/93‑1 (MQ=255)

                                                                                   aCCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTGGTCTCGGCAATATTCCGCCGCATCTGGc     >  1:381060/1‑93 (MQ=255)

                                                                                    ccTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTGGTCTCGGCAATATTCCGCCGCATCTGGCt    >  1:2278249/1‑93 (MQ=255)

                                                                                    ccTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTGGTCTCGGCAATATTCCGCCGCATCTGGCt    <  1:3514514/93‑1 (MQ=255)

                                                                                     cTTTCCTCGGCCCAGGCGCAGATATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTGGTCTCGGCAATATTCCGCCGCATCTGGCtt   >  1:2616102/1‑93 (MQ=255)

                                                                                      tttCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTGGTCTCGGCAATATTCCGCCGCATCTGGCttt  >  2:3402616/1‑93 (MQ=255)

                                                                                      tttCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTGGTCTCGGCAATATTCCGCCGCATCTGGCttt  <  1:650679/93‑1 (MQ=255)

ATTGCCCACGGTCTTGCTCCGCTGGCGAGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTGTTCCTTCCCACTGCTTTCACCTTTCTTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTGGTCTCGGCAATATTCCGCCGCATCTGGCTTT  >  NC_000913/2825012‑2825190

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 23 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`