BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	293,176	C→T	R283K (AGA→AAA)	yagM ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	293,176	0	C	T	99.5%	715.0 / NA	196	R283K (AGA→AAA)	yagM	hypothetical protein
Reads supporting (aligned to +/- strand): ref base C (0/0); major base T (114/81); minor base A (1/0); total (115/81)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 196 total aligned reads displayed.

ATAGAGAGTGATTGCCAGCGATACTTTAACTGGTAAAGTAGTATTTTACCTCTCATAATTGTTTGTCCTTCTTTGTTACCTCTTAGGTTCTGCTCTCTTGTGAGTATGGGTTTATAATGATGTCATCCGGTACATTAACACGGTTGGAAACATCTGTTTTGCGGAGCACTGGCAGCATA  >  NC_000913/293087‑293265

aTAGAGAGTGATTGCCAGCGATACTTTAACTGGTAAAGTAGTATTTTACCTCTCATAATTGTTTGTCCTTCTTTGTTACCTCTTAGGTTTTGc                                                                                        >  1:3648012/1‑93 (MQ=255)

aTAGAGAGTGATTGCCAGCGATACTTTAACTGGTAAAGTAGTATTTTACCTCTCATAATTGTTTGTCCTTCTTTGTTACCTCTTAGGTTTTGc                                                                                        >  1:1581995/1‑93 (MQ=255)

 tAGAGAGTGATTGCCAGCGATACTTTAACTGGTAAAGTAGTATTTTACCTCTCATAATTGTTTGTCCTTCTTTGTTACCTCTTAGGTTTTGct                                                                                       >  2:1085204/1‑93 (MQ=255)

 tAGAGAGTGATTGCCAGCGATACTTTAACTGGTAAAGTAGTATTTTACCTCTCATAATTGTTTGTCCTTCTTTGTTACCTCTTAGGTTTTGct                                                                                       <  1:1677969/93‑1 (MQ=255)

    agagTGATTGCCAGCGATACTTTAACTGGTAAAGTAGTATTTTACCTCTCATAATTGTTTGTCCTTCTTTGTTACCTCTTAGGTTTTGctctc                                                                                    <  2:2272637/93‑1 (MQ=255)

    agagTGATTGCCAGCGATACTTTAACTGGTAAAGTAGTATTTTACCTCTCATAATTGTTTGTCCTTCTTTGTTACCTCTTAGGTTTTGctctc                                                                                    <  2:3341054/93‑1 (MQ=255)

     gagTGATTGCCAGCGATACTTTAACTGGTAAAGTAGTATTTTACCTCTCATAATTGTTTGTCCTTCTTTGTTACCTCTTAGGTTTTGctctct                                                                                   >  2:2500808/1‑93 (MQ=255)

     gagTGATTGCCAGCGATACTTTAACTGGTAAAGTAGTATTTTACCTCTCATAATTGTTTGTCCTTCTTTGTTACCTCTTAGGTTTTGctctct                                                                                   >  1:3002374/1‑93 (MQ=255)

     gagTGATTGCCAGCGATACTTTAACTGGTAAAGTAGTATTTTACCTCTCATAATTGTTTGTCCTTCTTTGTTACCTCTTAGGTTTTGctctct                                                                                   <  2:1976757/93‑1 (MQ=255)

        tGATTGCCAGCGATACTTTAACTGGTAAAGTAGTATTTTACCTCTCATAATTGTTTGTCCTTCTTTGTTACCTCTTAGGTTTTGCTCTCTtgt                                                                                <  2:2851626/93‑1 (MQ=255)

          aTTGCCAGCGATACTTTAACTGGTAAAGTAGTATTTTACCTCTCATAATTGTTTGTCCTTCTTTGTTACCTCTTAGGTTTTGCTCTCTTGTGa                                                                              >  2:4100013/1‑93 (MQ=255)

              ccAGCGATACTTTAACTGGTAAAGTAGTATTTTACCTCTCATAATTGTTTGTCCTTCTTTGTTACCTCTTAGGTTTTGCTCTCTTGTGAGTAt                                                                          <  1:4191343/93‑1 (MQ=255)

               cAGCGATACTTTAACTGGTAAAGTAGTATTTTACCTCTCATAATTGTTTGTCCTTCTTTGTTACCTCTTAGGTTTTGCTCTCTTGTGAGTATg                                                                         <  2:2439272/93‑1 (MQ=255)

               cAGCGATACTTTAACTGGTAAAGTAGTATTTTACCTCTCATAATTGTTTGTCCTTCTTTGTTACCTCTTAGGTTTTGCTCTCTTGTGAGTATg                                                                         <  1:544652/93‑1 (MQ=255)

                aGCGATACTTTAACTGGTAAAGTAGTATTTTACCTCTCATAATTGTTTGTCCTTCTTTGTTACCTCTTAGGTTTTGCTCTCTTGTGAGTATgg                                                                        <  2:4656861/93‑1 (MQ=255)

                   gATACTTTAACTGGTAAAGTAGTATTTTACCTCTCATAATTGTTTGTCCTTCTTTGTTACCTCTTAGGTTTTGCTCTCTTGTGAGTATGGGtt                                                                     >  2:4029808/1‑93 (MQ=255)

                     tACTTTAACTGGTAAAGTAGTATTTTACCTCTCATAATTGTTTGTCCTTCTTTGTTACCTCTTAGGTTTTGCTCTCTTGTGAGTATGGGTTta                                                                   >  2:3943526/1‑93 (MQ=255)

                     tACTTTAACTGGTAAAGTAGTATTTTACCTCTCATAATTGTTTGTCCTTCTTTGTTACCTCTTAGGTTTTGCTCTCTTGTGAGTATGGGTTta                                                                   >  1:1564593/1‑93 (MQ=255)

                     tACTTTAACTGGTAAAGTAGTATTTTACCTCTCATAATTGTTTGTCCTTCTTTGTTACCTCTTAGGTTTTGCTCTCTTGTGAGTATGGGTTta                                                                   >  2:1340999/1‑93 (MQ=255)

                     tACTTTAACTGGTAAAGTAGTATTTTACCTCTCATAATTGTTCGTCCTTCTTTGTTACCTCTTAGGTTTTGCTCTCTTGTGAGTATGGGTTta                                                                   >  1:3303374/1‑93 (MQ=255)

                      aCTTTAACTGGTAAAGTAGTATTTTACCTCTCATAATTGTTTGTCCTTCTTTGTTACCTCTTAGGTTTTGCTCTCTTGTGAGTATGGGTTtat                                                                  >  2:2940358/1‑93 (MQ=255)

                      aCTTTAACTGGTAAAGTAGTATTTTACCTCTCATAATTGTTTGTCCTTCTTTGTTACCTCTTAGGTTTTGCTCTCTTGTGAGTATGGGTTtat                                                                  >  1:1054131/1‑93 (MQ=255)

                      aCTTTAACTGGTAAAGTAGTATTTTACCTCTCATAATTGTTTGTCCTTCTTTGTTACCTCTTAGGTTTTGCTCTCTTGTGAGTATGGGTTtat                                                                  >  1:474394/1‑93 (MQ=255)

                      aCTTTAACTGGTAAAGTAGTATTTTACCTCTCATAATTGTTTGTCCTTCTTTGTTACCTCTTAGGTTTTGCTCTCTTGTGAGTATGGGTTtat                                                                  <  1:1179479/93‑1 (MQ=255)

                       cTTTAACTGGTAAAGTAGTATTTTACCTCTCATAATTGTTTGTCCTTCTTTGTTACCTCTTAGGTTTTGCTCTCTTGTGAGTATGGGTTtata                                                                 >  2:977916/1‑93 (MQ=255)

                       cTTTAACTGGTAAAGTAGTATTTTACCTCTCATAATTGTTTGTCCTTCTTTGTTACCTCTTAGGTTTTGCTCTCTTGTGAGTATGGGTTtata                                                                 >  1:3600806/1‑93 (MQ=255)

                        tttAACTGGTAAAGTAGTATTTTACCTCTCATAATTGTTTGTCCTTTTTTGTTACCCCTTGGGTTTTGTTTTTTTGTGAGATTGGGTTtttat                                                                >  1:2622959/1‑92 (MQ=255)

                        tttAACTGGTAAAGTAGTATTTTACCTCTCATAATTGTTTGTCCTTCTTTGTTACCTCTTAGGTTTTGCTCTCTTGTGAGTATGGGTTTATaa                                                                >  1:3869367/1‑93 (MQ=255)

                        tttAACTGGTAAAGTAGTATTTTACCTCTCATAATTGTTTGTCCTTCTTTGTTACCTCTTAGGTTTTGCTCTCTTGTGAGTATGGGTTTATaa                                                                >  1:842457/1‑93 (MQ=255)

                          tAACTGGTAAAGTAGTATTTTTCCTCTCATAATTGTTTGTCCTTCTTTGTTACCTCTTAGGTTTTGCTCTCTTGTGAGTATGGGGTTATAatg                                                              >  2:2218075/1‑93 (MQ=255)

                           aaCTGGTAAAGTAGTATTTTACCTCTCATAATTGTTTGTCCTTCTTTGTTACCTCTTAGGTTTTGCTCTCTTGTGAGTATGGGTTTATAatga                                                             <  1:1833279/93‑1 (MQ=255)

                           aaCTGGTAAAGTAGTATTTTACCTCTCATAATTGTTTGTCCTTCTTTGTTACCTCTTAGGTTTTGCTCTCTTGTGAGTATGGGTTTATAatga                                                             >  2:2155114/1‑93 (MQ=255)

                           aaCTGGTAAAGTAGTATTTTACCTCTCATAATTGTTTGTCCTTCTTTGTTACCTCTTAGGTTTTGCTCTCTTGTGAGTATGGGTTTATAatga                                                             <  2:1376758/93‑1 (MQ=255)

                              tGGTAAAGTAGTATTTTACCTCTCATAATTGTTTGTCCTTCTTTGTTACCTCTTAGGTTTTGCTCTCTTGTGAGTATGGGTTTATAATGATGt                                                          <  1:3173254/93‑1 (MQ=255)

                              tGGTAAAGTAGTATTTTACCTCTCATAATTGTTTGTCCTTCTTTGTTACCTCTTAGGTTTTGCTCTCTTGTGAGTATGGGTTTATAATGATGt                                                          <  1:1018058/93‑1 (MQ=255)

                              tGGTAAAGTAGTATTTTACCTCTCATAATTGTTTGTCCTTCTTTGTTACCTCTTAGGTTTTGCTCTCTTGTGAGTATGGGTTTATAATGATGt                                                          >  1:3170431/1‑93 (MQ=255)

                              tGGTAAAGTAGTATTTTACCTCTCATAATTGTTTGTCCTTCTTTGTTACCTCTTAGGTTTTGCTCTCTTGTGAGTATGGGTTTATAATGATGt                                                          >  1:3570518/1‑93 (MQ=255)

                                  aaaGTAGTATTTTACCTCTCATAATTGTTTGTCCTTCTTTGTTACCTCTTAGGTTTTGCTCTCTTGTGAGTATGGGTTTATAATGATGTCATc                                                      >  1:4281990/1‑93 (MQ=255)

                                   aaGTAGTATTTTACCTCTCATAATTGTTTGTCCTTCTTTGTTACCTCTTAGGTTTTGCTCTCTTGTGAGTATGGGTTTATAATGATGTCATcc                                                     <  1:2802200/93‑1 (MQ=255)

                                    agtagtATTTTACCTCTCATAATTGTTTGTCCTTCTTTGTTACCTCTTAGGTTTTGCTCTCTTGTGAGTATGGGTTTATAATGATGTCATCCg                                                    <  2:3995177/93‑1 (MQ=255)

                                    agtagtATTTTACCTCTCATAATTGTTTGTCCTTCTTTGTTACCTCTTAGGTTTTGCTCTCTTGTGAGTATGGGTTTATAATGATGTCATCCg                                                    >  1:3111787/1‑93 (MQ=255)

                                    agtagtATTTTACCTCTCATAATTGTTTGTCCTTCTTTGTTACCTCTTAGGTTTTGCTCTCTTGTGAGTATGGGTTTATAATGATGTCATCCg                                                    >  1:1960791/1‑93 (MQ=255)

                                      tagtaTTTTACCTCTCATAATTGTTTGTCCTTCTTTGTTACCTCTTAGGTTTTGCTCTCTTGTGAGTATGGGTTTATAATGATGTCATCCGGt                                                  >  2:1127051/1‑93 (MQ=255)

                                        gtaTTTTACCTCTCATAATTGTTTGTCCTTCTTTGTTACCTCTTAGGTTTTGCTCTCTTGTGAGTATGGGTTTATAATGATGTCATCCGGTAc                                                >  1:2713791/1‑93 (MQ=255)

                                         taTTTTACCTCTCATAATTGTTTGTCCTTCTTTGTTACCTCTTAGGTTTTGCTCTCTTGTGAGTATGGGTTTATAATGATGTCATCCGGTACa                                               <  1:4336272/93‑1 (MQ=255)

                                         taTTTTACCTCTCATAATTGTTTGTCCTTCTTTGTTACCTCTTAGGTTTTGCTCTCTTGTGAGTATGGGTTTATAATGATGTCATCCGGTACa                                               <  1:696934/93‑1 (MQ=255)

                                         taTTTTACCTCTCATAATTGTTTGTCCTTCTTTGTTACCTCTTAGGTTTTGCTCTCTTGTGAGTATGGGTTTATAATGATGTCATCCGGTACa                                               >  2:4340277/1‑93 (MQ=255)

                                          aTTTTACCTCTCATAATTGTTTGTCCTTCTTTGTTACCTCTTAGGTTTTGCTCTCTTGTGTGTATGGGTTTATAATGATGTCATCCGGTACAt                                              >  2:3479635/1‑93 (MQ=255)

                                          aTTTTACCTCTCATAATTGTTTGTCCTTCTTTGTTACCTCTTAGGTTTTGCTCTCTTGTGAGTATGGGTTTATAATGATGTCATCCGGTACAt                                              >  2:2637733/1‑93 (MQ=255)

                                           ttttACCTCTCATAATTGTTTGTCCTTCTTTGTTACCTCTTAGGTTTTGCTCTCTTGTGAGTATGGGTTTATAATGATGTCATCCGGTACAtt                                             <  1:1917384/93‑1 (MQ=255)

                                           ttttACCTCTCATAATTGTTTGTCCTTCTTTGTTACCTCTTAGGTTTTGCTCTCTTGTGAGTATGGGTTTATAATGATGTCATCCGGTACAtt                                             >  1:2582309/1‑93 (MQ=255)

                                           ttttACCTCTCATAATTGTTTGTCCTTCTTTGTTACCTCTTAGGTTTTGCTCTCTTGTGAGTATGGGTTTATAATGATGTCATCCGGTACAtt                                             <  2:605699/93‑1 (MQ=255)

                                           ttttACCTCTCATAATTGTTTGTCCTTCTTTGTTACCTCTTAGGTTTTGCTCTCTTGTGAGTATGGGTTTATAATGATGTCATCCGGTACAtt                                             <  1:2397377/93‑1 (MQ=255)

                                            tttACCTCTCATAATTGTTTGTCCTTCTTTGTTACCTCTTAGGTTTTGCTCTCTTGTGAGTATGGGTTTATAATGATGTCATCCGGTACATTa                                            <  1:2173048/93‑1 (MQ=255)

                                             ttACCTCTCATAATTGTTTGTCCTTCTTTGTTACCTCTTAGGTTTTGCTCTCTTGTGAGTATGGGTTTATAATGATGTCATCCGGTACATTaa                                           <  1:3351906/93‑1 (MQ=255)

                                               aCCTCTCATAATTGTTTGTCCTTCTTTGTTACCTCTTAGGTTTTGCTCTCTTGTGAGTATGGGTTTATAATGATGTCATCCGGTACATTAaca                                         >  1:1751361/1‑93 (MQ=255)

                                                ccTCTCATAATTGTTTGTCCTTCTTTGTTACCTCTTAGGTTTTGCTCTCTTGTGAGTATGGGTTTATAATGATGTCATCCGGTACATTAacac                                        >  1:4186158/1‑93 (MQ=255)

                                                ccTCTCATAATTGTTTGTCCTTCTTTGTTACCTCTTAGGTTTTGCTCTCTTGTGAGTATGGGTTTATAATGATGTCATCCGGTACATTAacac                                        <  2:3266949/93‑1 (MQ=255)

                                                ccTCTCATAATTGTTTGTCCTTCTTTGTTACCTCTTAGGTTTTGCTCTCTTGTGAGTATGGGTTTATAATGATGTCATCCGGTACATTAacac                                        <  2:3304959/93‑1 (MQ=255)

                                                ccTCTCATAATTGTTTGTCCTTCTTTGTTACCTCTTAGGTTTTGCTCTCTTGTGAGTATGGGTTTATAATGATGTCATCCGGTACATTAacac                                        >  1:2346369/1‑93 (MQ=255)

                                                 ctctCATAATTGTTTGTCCTTCTTTGTTACCTCTTAGGTTTTGCTCTTTTGTGAGTATGGGTTTATAATGATGTCATCCGGTACATTAACACg                                       <  2:1940877/93‑1 (MQ=255)

                                                 ctctCATAATTGTTTGTCCTTCTTTGTTACCTCTTAGGTTTTGCTCTCTTGTGAGTATGGGTTTATAATGATGTCATCCGGTACATTAACACg                                       >  2:4165419/1‑93 (MQ=255)

                                                 ctctCATAATTGTTTGTCCTTCTTTGTTACCTCTTAGGTTTTGCTCTCTTGTGAGTATGGGTTTATAATGATGTCATCCGGTACATTAACACg                                       >  2:3813284/1‑93 (MQ=255)

                                                 ctctCATAATTGTTTGTCCTTCTTTGTTACCTCTTAGGTTTTGCTCTCTTGTGAGTATGGGTTTATAATGATGTCATCCGGTACATTAACACg                                       >  1:3687558/1‑93 (MQ=255)

                                                  tctcATAATTGTTTGTCCTTCTTTGTTACCTCTTAGGTTTTGCTCTCTTGTGAGTATGGGTTTATAATGATGTCATCCGGTACATTAACACgg                                      >  1:2958844/1‑93 (MQ=255)

                                                  tctcATAATTGTTTGTCCTTCTTTGTTACCTCTTAGGTTTTGCTCTCTTGTGAGTATGGGTTTATAATGATGTCATCCGGTACATTAACACgg                                      >  2:1889175/1‑93 (MQ=255)

                                                   ctcATAATTGTTTGTCCTTCTTTGTTACCTCTTAGGTTTTGCTCTCTTGTGAGTATGGGTTTATAATGATGTCATCCGGTACATTAACACGGt                                     >  1:3802672/1‑93 (MQ=255)

                                                   ctcATAATTGTTTGTCCTTCTTTGTTACCTCTTAGGTTTTGCTCTCTTGTGAGTATGGGTTTATAATGATGTCATCCGGTACATTAACACGGt                                     <  1:4485031/93‑1 (MQ=255)

                                                   ctcATAATTGTTTGTCCTTCTTTGTTACCTCTTAGGTTTTGCTCTCTTGTGAGTATGGGTTTATAATGATGTCATCCGGTACATTAACACGGt                                     <  1:3474495/93‑1 (MQ=255)

                                                    tcATAATTGTTTGTCCTTCTTTGTTACCTCTTAGGTTTTGCTCTCTTGTGAGTATGGGTTTATAATGATGTCATCCGGTACATTAACACGGtt                                    <  2:2338466/93‑1 (MQ=255)

                                                    tcATAATTGTTTGTCCTTCTTTGTTACCTCTTAGGTTTTGCTCTCTTGTGAGTATGGGTTTATAATGATGTCATCCGGTACATTAACACGGtt                                    >  2:3358972/1‑93 (MQ=255)

                                                    tcATAATTGTTTGTCCTTCTTTGTTACCTCTTAGGTTTTGCTCTCTTGTGAGTATGGGTTTATAATGATGTCATCCGGTACATTAACACGGtt                                    <  2:1736995/93‑1 (MQ=255)

                                                       tAATTGTTTGTCCTTCTTTGTTACCTCTTAGGTTTTGCTCTCTTGTGAGTATGGGTTTATAATGATGTCATCCGGTACATTAACACGGTTGGa                                 >  1:4068195/1‑93 (MQ=255)

                                                         aTTGTTTGTCCTTCTTTGTTACCTCTTAGGTTTTGCTCTCTTGTGAGTATGGGTTTATAATGATGTCATCCGGTACATTAACACGGTTGGaaa                               >  1:149692/1‑93 (MQ=255)

                                                             tttgtCCTTCTTTGTTACCTCTTAGGTTTTGCTCTCTTGTGAGTATGGGTTTATAATGATGTCATCCGGTACATTAACACGGTTGGAAACATc                           <  2:4226283/93‑1 (MQ=255)

                                                              ttgtCCTTCTTTGTTACCTCTTAGGTTTTGCTCTCTTGTGAGTATGGGTTTATAATGATGTCATCCGGTACATTAACACGGTTGGAAACATCt                          <  2:3176258/93‑1 (MQ=255)

                                                               tgtCCTTCTTTGTTACCTCTTAGGTTTTGCTCTCTTGTGAGTATGGGTTTATAATGATGTCATCCGGTACATTAACACGGTTGGAAACATCTg                         <  2:832815/93‑1 (MQ=255)

                                                                gtCCTTCTTTGTTACCTCTTAGGTTTTGCTCTCTTGTGAGTATGGGTTTATAATGATGTCATCCGGTACATTAACACGGTTGGAAACATCTGt                        >  2:1111790/1‑93 (MQ=255)

                                                                gtCCTTCTTTGTTACCTCTTAGGTTTTGCTCTCTTGTGAGTATGGGTTTATAATGATGTCATCCGGTACATTAACACGGTTGGAAACATCTGt                        <  2:4447120/93‑1 (MQ=255)

                                                                 tCCTTCTTTGTTACCTCTTAGGTTTTGCTCTCTTGTGAGTATGGGTTTATAATGATGTCATCCGGTACATTAACACGGTTGGAAACATCTGtt                       >  2:4116792/1‑93 (MQ=255)

                                                                  ccTTCTTTGTTACCTCTTAGGTTTTGCTCTCTTGTGAGTATGGGTTTATAATGATGTCATCCGGTACATTAACACGGTTGGAAACATCTGttt                      <  2:3753311/93‑1 (MQ=255)

                                                                  ccTTCTTTGTTACCTCTTAGGTTTTGCTCTCTTGTGAGTATGGGTTTATAATGATGTCATCCGGTACATTAACACGGTTGGAAACATCTGttt                      >  1:1567237/1‑93 (MQ=255)

                                                                   cttcttTGTTACCTCTTAGGTTTTGCTCTCTTGTGAGTATGGGTTTATAATGATGTCATCCGGTACATTAACACGGTTGGAAACATCTGtttt                     <  2:2613507/93‑1 (MQ=255)

                                                                   cttcttTGTTACCTCTTAGGTTTTGCTCTCTTGTGAGTATGGGTTTATAATGATGTCATCCGGTACATTAACACGGTTGGAAACATCTGtttt                     >  1:972854/1‑93 (MQ=255)

                                                                    ttcttTGTTACCTCTTAGGTTTTGCTCTCTTGTGAGTATGGGTTTATAATGATGTCATCCGGTACATTAACACGGTTGGAAACATCTGTTTTg                    <  2:4521500/93‑1 (MQ=255)

                                                                      cttTGTTACCTCTTAGGTTTTGCTCTCTTGTGAGTATGGGTTTATAATGATGTCATCCGGTACATTAACACGGTTGGAAACATCTGTTTTGCg                  >  2:1225039/1‑93 (MQ=255)

                                                                       tttGTTACCTCTTAGGTTTTGCTCTCTTGTGAGTATGGGTTTATAATGATTTCATCCGGTACATTAACACGGTTGGAAACATCTGTTTTGCgg                 >  2:2723580/1‑93 (MQ=255)

                                                                         tGTTACCTCTTAGGTTTTGCTCTCTTGTGAGTATGGGTTTATAATGATGTGATCCGGTACATTAACACGGTTGGAAACATCTGTTTTGCGGAg               <  1:2515871/93‑1 (MQ=255)

                                                                            tACCTCTTAGGTTTTGCTCTCTTGTGAGTATGGGTTTATAATGATGTCATCCGGTACATTAACACGGTTGGAAACATCTGTTTTGCGGAGCAc            >  2:377293/1‑93 (MQ=255)

                                                                             aCCTCTTAGGTTTTGCTCTCTTGTGAGTATGGGTTTATAATGATGTCATCCGGTACATTAACACGGTTGGAAACATCTGTTTTGCGGAGCACt           <  1:1725294/93‑1 (MQ=255)

                                                                             aCCTCTTAGGTTTTGCTCTCTTGTGAGTATGGGTTTATAATGATGTCATCCGGTACATTAACACGGTTGGAAACATCTGTTTTGCGGAGCACt           <  1:4547155/93‑1 (MQ=255)

                                                                              ccTCTTAGGTTTTGCTCTCTTGTGAGTATGGGTTTATAATGATGTCATCCGGTACATTAACACGGTTGGAAACATCTGTTTTGCGGAGCACTg          <  2:4647123/93‑1 (MQ=255)

                                                                              ccTCTTAGGTTTTGCTCTCTTGTGAGTATGGGTTTATAATGATGTCATCCGGTACATTAACACGGTTGGAAACATCTGTTTTGCGGAGCACTg          >  1:492309/1‑93 (MQ=255)

                                                                               ctctTAGGTTTTGCTCTCTTGTGAGTATGGGTTTATAATGATGTCATCCGGTACATTAACACGGTTGGAAACATCTGTTTTGCGGAGCACTgg         >  1:2898295/1‑93 (MQ=255)

                                                                               ctctTAGGTTTTGCTCTCTTGTGAGTATGGGTTTATAATGATGTCATCCGGTACATTAACACGGTTGGAAACATCTGTTTTGCGGAGCACTgg         <  1:3200097/93‑1 (MQ=255)

                                                                                tctTAGGTTTTGCTCTCTTGTGAGTATGGGTTTATAATGATGTCATCCGGTACATTAACACGGTTGGAAACATCTGTTTTGCGGAGCACTGgc        >  2:2987146/1‑93 (MQ=255)

                                                                                tctTAGGTTTTGCTCTCTTGTGAGTATGGGTTTATAATGATGTCATCCGGTACATTAACACGGTTGGAAACATCTGTTTTGCGGAGCACTGgc        >  2:2532955/1‑93 (MQ=255)

                                                                                 ctTAGGTTATGCTCTCTTGTGAGTATGGGTTTATAATGATGTCATCCGGTACATTAACACGGTTGGAAACATCTGTTTTGCGGAGCACTGgca       >  2:4573580/1‑93 (MQ=255)

                                                                                  ttAGGTTTTGCTCTCTTGTGAGTATGGGTTTATAATGATGTCATCCGGTACATTAACACGGTTGGAAACATCTGTTTTGCGGAGCACTGgcag      <  2:2072519/93‑1 (MQ=255)

                                                                                      gTTTTGCTCTCTTGTGAGTATGGGTTTATAATGATGTCATCCGGTACATTAACACGGTTGGAAACATCTGTTTTGCGGAGCACTGGCAGCATa  >  2:1657249/1‑93 (MQ=255)

ATAGAGAGTGATTGCCAGCGATACTTTAACTGGTAAAGTAGTATTTTACCTCTCATAATTGTTTGTCCTTCTTTGTTACCTCTTAGGTTCTGCTCTCTTGTGAGTATGGGTTTATAATGATGTCATCCGGTACATTAACACGGTTGGAAACATCTGTTTTGCGGAGCACTGGCAGCATA  >  NC_000913/293087‑293265

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 31 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`