BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,844,596	A→G	R280R (CGT→CGC)	hycD ←	membrane‑spanning protein of hydrogenase 3

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,844,596	0	A	G	100.0%	448.0 / NA	145	R280R (CGT→CGC)	hycD	membrane‑spanning protein of hydrogenase 3
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (70/75); total (70/75)

Only 100 of 153 total aligned reads displayed.

TTTAATCACGCCGCCAGCAAGGAGACGAACGCTAAAAATGCAAAGCCAAACCCAGCCCAGGTAATGCGCGGAGTAATATCAAGACGCAGACGGGCCATGCTGTTTTCGAACAGCGCGATAACCAGGACGCCGACCACCAGTTTTACGATGGCAATCACCAGCGCCAGCAGCAGTCCACC  >  NC_000913/2844507‑2844685

tttAATCACGCCGCCAGCAAGGAGACGAACGCTAAAAATGCAAAGCCAAACCCAGCCCAGGTAATGCGCGGAGTAATATCAAGACGCAGGCgg                                                                                        >  2:2162043/1‑93 (MQ=255)

   aaTCACGCCGCCAGCAAGGAGACGAACGCTAAAAATGCAAAGCCAAACCCAGCCCAGGTAATGCGCGGAGTAATATCAAGACGCAGGCGGGcc                                                                                     >  2:1009998/1‑93 (MQ=255)

    aTCACGCCGCCAGCAAGGAGACGAACGCTAAAAATGCAAAGCCAAACCCAGCCCAGGTAATGCGCGGAGTAATATCAAGACGCAGGCGGGCCa                                                                                    <  1:3175440/93‑1 (MQ=255)

    aTCACGCCGCCAGCAAGGAGACGAACGCTAAAAATGCAAAGCCAAACCCAGCCCAGGTAATGCGCGGAGTAATATCAAGACGCAGGCGGGCCa                                                                                    >  1:54833/1‑93 (MQ=255)

       aCGCCGCCAGCAAGGAGACGAACGCTAAAAATGCAAAGCCAAACCCAGCCCAGGTAATGCGCGGAGTAATATCAAGACGCAGGCGGGCCATGc                                                                                 <  1:2636155/93‑1 (MQ=255)

       aCGCCGCCAGCAAGGAGACGAAAGCTAAAAATGCAAAGCCAAACCCAGCCCAGGTAATGCGCGGAGTAATATCAAGACGCAGGCGGGCCACGc                                                                                 >  2:2815441/1‑93 (MQ=255)

         gccgccAGCAAGGAGACGAACGCTAAAAATGCAAAGCCAAACCCAGCCCAGGTAATGCGCGGAGTAATATCAAGACGCAGGCGGGCCATGCTg                                                                               <  1:338665/93‑1 (MQ=255)

          ccgccAGCAAGGAGACGAACGCTAAAAATGCAAAGCCAAACCCAGCCCAGGTAATGCGCGGAGTAATATCAAGACGCAGGCGGGCCATGCTGt                                                                              >  2:473328/1‑93 (MQ=255)

            gccAGCAAGGAGACGAACGCTAAAAATGCAAAGCCAAACCCAGCCCAGGTAATGCGCGGAGTAATATCAAGACGCAGGCGGGCCATGCTGttt                                                                            >  2:633212/1‑93 (MQ=255)

            gccAGCAAGGAGACGAACGCTAAAAATGCAAAGCCAAACCCAGCCCAGGTAATGCGCGGAGTAATATCAAGACGCAGGCGGGCCATGCTGttt                                                                            <  2:1656979/93‑1 (MQ=255)

            cccAGCAAGGAGACGAACCCTAAAAATGCAAAGCCACACCCAGCCCAGGTAATGCGCGGAGTAATATCAAGACGCAGGCGGGCCATGCTGttt                                                                            <  1:976800/92‑1 (MQ=255)

             ccAGCAAGGAGACGAACGCTAAAAATGCAAAGCCAAACCCAGCCCAGGTAATGCGCGGAGTAATATCAAGACGCAGGCGGGCCATGCTGtttt                                                                           >  2:1381666/1‑93 (MQ=255)

             ccAGCAAGGAGACGAACGCTAAAAATGCAAAGCCAAACCCAGCCCAGGTAATGCGCGGAGTAATATCAAGACGCAGGCGGGCCATGATGtttt                                                                           >  2:153455/1‑93 (MQ=255)

              cAGCAAGGAGACGAACGCTAAAAATGCAAAGCCAAACCCAGCCCAGGTAATGCGCGGAGTAATATCAAGACGCAGGCGGGCCATGCTGTTTTc                                                                          <  2:2827938/93‑1 (MQ=255)

                gCAAGGAGACGAACGCTAAAAATGCAAAGCCAAACCCAGCCCAGGTAATGCGCGGAGTAATATCAAGACGCAGGCGGGCCATGCTGTTTTCGa                                                                        >  2:2881858/1‑93 (MQ=255)

                  aaGGAGACGAACGCTAAAAATGCAAAGCCAAACCCAGCCCAGGTAATGCGCGGAGTAATATCAAGACGCAGGCGGGCCATGCTGTTTTTGAAc                                                                      >  1:2857804/1‑93 (MQ=255)

                  aaGGAGACGAACGCTAAAAATGCAAAGCCAAACCCAGCCCAGGTAATGCGCGGAGTAATATCAAGACGCAGGCGGGCCATGCTGTTTTCGAAc                                                                      >  1:1364553/1‑93 (MQ=255)

                    ggAGACGAACGCTAAAAATGCAAAGCCAAACCCAGCCCAGGTAATGCGCGGAGTAATATCAAGACGCAGGCGGGCCATGCTGTTTTCGAACAg                                                                    <  1:3753996/93‑1 (MQ=255)

                     gagaCGAACGCTAAAAATGCAAAGCCAAACCCAGCCCAGGTAATGCGCGGAGTAATATCAAGACGCAGGCGGGCCATGCTGTTTTCGAACAgc                                                                   <  1:1202829/93‑1 (MQ=255)

                       gaCGAACGCTAAAAATGCAAAGCCAAACCCAGCCCAGGTAATGCGCGGAGTAATATCAAGACGCAGGCGGGCCATGCTGTTTTCGAACAgcgc                                                                 <  2:3009305/93‑1 (MQ=255)

                        aCGAACGCTAAAAATGCAAAGCCAAACCCAGCCCAGGTAATGCGCGGAGTAATATCAAGACGCAGGCGGGCCATGCTGTTTTCGAACAGcgcg                                                                >  2:190831/1‑93 (MQ=255)

                         cGAACGCTAAAAATGCAAAGCCAAACCCAGCCCAGGTAATGCGCGGAGTAATATCAAGACGCAGGCGGGCCATGCTGTTTTCGAACAGCGCGa                                                               <  1:2639791/93‑1 (MQ=255)

                         cGAACGCTAAAAATGCAAAGCCAAACCCAGCCCAGGTAATGCGCGGAGTAATATCAAGACGCAGGCGGGCCATGCTGTTTTCGAACAGCGCGa                                                               <  1:2936549/93‑1 (MQ=255)

                           aaCGCTAAAAATGCAAAGCCAAACCCAGCCCAGGTAATGCGCGGAGTAATATCAAGACGCAGGCGGGCCATGCTGTTTTCGAACAGCGCGATa                                                             <  2:926835/93‑1 (MQ=255)

                            aCGCTAAAAATGCAAAGCCAAACCCAGCCCAGGTAATGCGCGGAGTAATATCAAGACGCAGGCGGGCCATGCTGTTTTCGAACAGCGCGATaa                                                            <  1:3210696/93‑1 (MQ=255)

                             cGCTAAAAATGCAAAGCCAAACCCAGCCCAGGTAATGCGCGGAGTAATATCAAGACGCAGGCGGGCCATGCTGTTTTCGAACAGCGCGATAAc                                                           <  1:1017485/93‑1 (MQ=255)

                              gCTAAAAATGCAAAGCCAAACCCAGCCCAGGTAATGCGCGGAGTAATATCAAGACGCAGGCGGGCCATGCTGTTTTCGAACAGCGCGATAAcc                                                          >  2:2741505/1‑93 (MQ=255)

                              gCTAAAAATGCAAAGCCAAACCCAGCCCAGGTAATGCGCGGAGTAATATCAAGACGCAGGCGGGCCATGCTGTTTTCGAACAGCGCGATAAcc                                                          >  1:83804/1‑93 (MQ=255)

                               cTAAAAATGCAAAGCCAAACCCAGCCCAGGTAATGCGCGGAGTAATATCAAGACGCAGGCGGGCCATGCTGTTTTCGAACAGCGCGATAACCa                                                         >  1:1197550/1‑93 (MQ=255)

                               cTAAAAATGCAAAGCCAAACCCAGCCCAGGTAATGCGCGGAGTAATATCAAGACGCAGGCGGGCCATGCTGTTTTCGAACAGCGCGATAACCa                                                         <  1:839764/93‑1 (MQ=255)

                               cTAAAAATGCAAAGCCAAACCCAGCCCAGGTAATGCGCGGAGTAATATCAAGACGCAGGCGGGCCATGCTGTTTTCGAACAGCGCGATAACCa                                                         <  1:1497641/93‑1 (MQ=255)

                                tAAAAATGCAAAGCCAAACCCAGCCCAGGTAATGCGCGGAGTAATATCAAGACGCAGGCGGGCCATGCTGTTTTCGAACAGCGCGATAACCAg                                                        <  2:4117946/93‑1 (MQ=255)

                                tAAAAATGCAAAGCCAAACCCAGCCCAGGTAATGCGCGGAGTAATATCAAGACGCAGGCGGGCCATGCTGTTTTCGAACAGCGCGATAACCAg                                                        <  2:1131534/93‑1 (MQ=255)

                                tAAAAATGCAAAGCCAAACCCAGCCCAGGTAATGCGCGGAGTAATATCAAGACGCAGGCGGGCCATGCTGTTTTCGAACAGCGCGATAACCAg                                                        >  2:3117603/1‑93 (MQ=255)

                                tAAAAATGCAAAGCCAAACCCAGCCCAGGTAATGCGCGGAGTAATATCAAGACGCAGGCGGCCCATGCTGTTTTCGAACAGCGCGATAACCAg                                                        <  1:3644464/93‑1 (MQ=255)

                                  aaaaTGCAAAGCCAAACCCAGCCCAGGTAATGCGCGGAGTAATATCAAGACGCAGGCGGGCCATGCTGTTTTCGAACAGCGCGATAACCAGGa                                                      <  2:4253353/93‑1 (MQ=255)

                                  aaaaTGCAAAGCCAAACCCAGCCCAGGTAATGCGCGGAGTAATATCAAGACGCAGGCGGGCCATGCTGTTTTCGAACAGCGCGATAACCAGGa                                                      <  2:3126126/93‑1 (MQ=255)

                                  aaaaTGCAAAGCCAAACCCAGCCCAGGTAATGCGCGGAGTAATATCAAGACGCAGGCGGGCCATGCTGTTTTCGAACAGCGCGATAACCAGGa                                                      <  2:839912/93‑1 (MQ=255)

                                  aaaaTGCAAAGCCAAACCCAGCCCAGGTAATGCGCGGAGTAATATCAAGACGCAGGCGGGCCATGCTGTTTTCGAACAGCGCGATAACCAGGa                                                      <  2:2466788/93‑1 (MQ=255)

                                  aaaaTGCAAAGCCAAACCCAGCCCAGGTAATGCGCGGAGTAATATCAAGACGCAGGCGGGCCATGCTGTTTTCGAACAGCGCGATAACCAGGa                                                      <  2:2477004/93‑1 (MQ=255)

                                   aaaTGCAAAGCCAAACCCAGCCCAGGTAATGCGCGGAGTAATATCAAGACGCAGGCGGGCCATGCTGTTTTCGAACAGCGCGATAACCAGGAc                                                     <  2:844894/93‑1 (MQ=255)

                                     aTGCAAAGCCAAACCCAGCCCAGGTAATGCGCGGAGTAATATCAAGACGCAGGCGGGCCATGCTGTTTTCGAACAGCGCGATAACCAGGACGc                                                   <  1:1583159/93‑1 (MQ=255)

                                     aTGCAAAGCCAAACCCAGCCCAGGTAATGCGCGGAGTAATAGCAGGCCGCAGGCGGGCCATGCTGTTTTCGAACAGCGCGATAACCAGGACGc                                                   <  1:4149091/93‑1 (MQ=255)

                                      tGCAAAGCCAAACCCAGCCCAGGTAATGCGCGGAGTAATATCAAGACGCAGGCGGGCCATGCTGTTTTCGAACAGCGCGATAACCAGGACGcc                                                  <  1:4603315/93‑1 (MQ=255)

                                      tGCAAAGCCAAACCCAGCCCAGGTAATGCGCGGAGTAATATCAAGACGCAGGCGGGCCATGCTGTTTTCGAACAGCGCGATAACCAGGACGcc                                                  <  2:2441061/93‑1 (MQ=255)

                                      tGCAAAGCCAAACCCAGCCCAGGTAATGCGCGGAGTAATATCAAGACGCAGGCGGGCCATGCTGTTTTCGAACAGCGCGATAACCAGGACGcc                                                  <  1:3618803/93‑1 (MQ=255)

                                       gCAAAGCCAAACCCAGACCAGCTAATGCGCGGAGTAATATCAAGACGCAGGCGGGCCATGCTGTTTTCGAACAGCGCGATAACCAGGACGCCg                                                 >  2:1741968/1‑93 (MQ=255)

                                        cAAAGCCAAACCCAGCCCAGGTAATGCGCGGAGTAATATCAAGACGCAGGCGGGCCATGCTGTTTTCGAACAGCGCGATAACCAGGACGCCGa                                                <  2:2426152/93‑1 (MQ=255)

                                              cAAGCCCAGCCAAGGTAATGCGCGGAGTAATATCAAGACGCAGGCGGGCCATGCTGTTTTCGAACAGCGCGATAACCAGGACGCCGAccacca                                          <  2:4513819/93‑1 (MQ=255)

                                              cAAACCCAGCCCAGGTAATGCGCGGAGTAATATCAAGACGCAGGCGGGCCATGCTGTTTTCGAACAGCGCGATAACCAGGACGCCGAccacca                                          >  1:4634153/1‑93 (MQ=255)

                                              cAAACCCAGCCCAGGTAATGCGCGGAGTAATATCAAGACGCAGGCGGGCCATGCTGTTTTCGAACAGCGCGATAACCAGGACGCCGAccacca                                          >  2:3238944/1‑93 (MQ=255)

                                               aaaCCCAGCCCAGGTAATGCGCGGAGTAATATCAAGACGCAGGCGGGCCATGCTGTTTTCGAACAGCGCGATAACCAGGACGCCGACCACCAg                                         >  1:3249742/1‑93 (MQ=255)

                                                    cagcccagGTAATGCGCGGAGTAATATCAAGACGCAGGCGGGCCATGCTGTTTTCGAACAGCGCGATAACCAGGACGCCGACCACCAGTTTTa                                    <  1:1853149/93‑1 (MQ=255)

                                                    cagcccagGTAATGCGCGGAGTAATATCAAGACGCAGGCGGGCCATGCTGTTTTCGAACAGCGCGATAACCAGGACGCCGACCACCAGTTTTa                                    <  2:664579/93‑1 (MQ=255)

                                                    cagcccagGTAATGCGCGGAGTAATATCAAGACGCAGGCGGGCCATGCTGTTTTCGAACAGCGCGATAACCAGGACGCCGACCACCAGTTTTa                                    <  1:4669440/93‑1 (MQ=255)

                                                     agcccagGTAATGCGCGGAGTAATATCAAGACGCAGGCGGGCCATGCTGTTTTCGAACAGCGCGATAACCAGGACGCCGACCACCAGTTTTAc                                   <  1:1937287/93‑1 (MQ=255)

                                                      gcccagGTAATGCGCGGAGTAATATCAAGACGCAGGCGGGCCATGCTGTTTTCGAACAGCGCGATAACCAGGACGCCGACCACCAGTTTTACg                                  >  2:1955939/1‑93 (MQ=255)

                                                       cccagGTAATGCGCGGAGTAATATCAAGACGCAGGCGGGCCATGCTGTTTTCGAACAGCGCGATAACCAGGACGCCGACCACCAGTTTTACGa                                 <  2:4392458/93‑1 (MQ=255)

                                                       cccagGTAATGCGCGGAGTAATATCAAGACGCAGGCGGGCCATGCTGTTTTCGAACAGCGCGATAACCAGGACGCCGACCACCAGTTTTACGa                                 <  2:275301/93‑1 (MQ=255)

                                                       cccagGTAATGCGCGGAGTAATATCAAGACGCAGGCGGGCCATGCTGTTTTCGAACAGCGCGATAACCAGGACGCCGACCACCAGTTTTACGa                                 <  2:656333/93‑1 (MQ=255)

                                                         cagGTAATGCGCGGAGTAATATCAAGACGCAGGCGGGCCATGCTGTTTTCGAACAGCGCGATAACCAGGACGCCGACCACCAGTTTTACGATg                               >  2:3337205/1‑93 (MQ=255)

                                                         cagGTAATGCGCGGAGTAATATCAAGACGCAGGCGGGCCATGCTGTTTTCGAACAGCGCGATAACCAGGACGCCGACCACCAGTTTTACGATg                               >  2:385046/1‑93 (MQ=255)

                                                         cagGTAATGCGCGGAGTAATATCAAGACGCAGGCGGGCCATGCTGTTTTCGAACAGCGCGATAACCAGGACGCCGACCACCAGTTTTACGATg                               >  1:4278873/1‑93 (MQ=255)

                                                         cagGTAATGCGCGGAGTAATATCAAGACGCAGGCGGGCCATGCTGTTTTCGAACAGCGCGATAACCAGGACGCCGACCACCAGTTTTACGATg                               >  2:169873/1‑93 (MQ=255)

                                                          agGTAATGCGCGGAGTAATATCAAGACGCAGGCGGGCCATGCTGTTTTCGAACAGCGCGATAACCAGGACGCCGACCACCAGTTTTACGATgg                              <  1:817898/93‑1 (MQ=255)

                                                          agGTAATGCGCGGAGTAATATCAAGACGCAGGCGGGCCATGCTGTTTTCGAACAGCGCGATAACCAGGACGCCGACCACCAGTTTTACGATgg                              >  1:657941/1‑93 (MQ=255)

                                                           ggTAATGCGCGGAGTAATATCAAGACGCAGGCGGGCCATGCTGTTTTCGAACAGCGCGATAACCAGGACGCCGACCACCAGTTTTACGATGGc                             >  1:2538341/1‑93 (MQ=255)

                                                           ggTAATGCGCGGAGTAATATCAAGACGCAGGCGGGCCATGCTGTTTTCGAACAGCGCGATAACCAGGACGCCGACCACCAGTTTTACGATGGc                             >  1:3117496/1‑93 (MQ=255)

                                                             tAATGCGCGGAGTAATATCAAGACGCAGGCGGGCCATGCTGTTTTCGAACAGCGCGATAACCAGGACGCCGACCACCAGTTTTACGATGGCaa                           <  2:3248567/93‑1 (MQ=255)

                                                             tAATGCGCGGAGTAATATCAAGACGCAGGCGGGCCATGCTGTTTTCGAACAGCGCGATAACCAGGACGCCGACCACCAGTTTTACGATGGCaa                           <  1:1857366/93‑1 (MQ=255)

                                                              aaTGCGCGGAGTAATATCAAGACGCAGGCGGGCCATGCTGTTTTCGAACAGCGCGATAACCAGGACGCCGACCACCAGTTTTACGATGGCAAt                          <  1:2791692/93‑1 (MQ=255)

                                                               aTGCGCGGAGTAATATCAAGACGCAGGCGGGCCATGCTGTTTTCGAACAGCGCGATAACCAGGACGCCGACCACCAGTTTTACGATGGCAATc                         <  1:2959837/93‑1 (MQ=255)

                                                               aTGCGCGGAGTAATATCAAGACGCAGGCGGGCCATGCTGTTTTCGAACAGCGCGATAACCAGGACGCCGACCACCAGTTTTACGATGGCAATc                         <  2:563777/93‑1 (MQ=255)

                                                               aTGCGCGGAGTAATATCAAGACGCAGGCGGGCCATGCTGTTTTCGAACAGCGCGATAACCAGGACGCCGACCACCAGTTTTACGATGGCAATc                         <  2:2387090/93‑1 (MQ=255)

                                                               aTGCGCGGAGTAATATCAAGACGCAGGCGGGCCATGCTGTTTTCGAACAGCGCGATAACCAGGACGCCGACCACCAGTTTTACGATGGCAATc                         <  1:1588951/93‑1 (MQ=255)

                                                               aTGCGCGGAGTAATATCAAGACGCAGGCGGGCCATGCTGTTTTCGAACAGCGCGATAACCAGGACGCCGACCACCAGTTTTACGATGGCAATc                         >  2:1989511/1‑93 (MQ=255)

                                                                 gcgcGGAGTAATATCAAGACGCAGGCGGGCCATGCTGTTTTCGAACAGCGCGATAACCAGGACGCCGACCACCAGTTTTACGATGGCAATCAc                       <  1:1875241/93‑1 (MQ=255)

                                                                     ggAGTAATATCAAGACGCAGGCGGGCCATGCTGTTTTCGAACAGCGCGATAACCAGGACGCCGACCACCAGTTTTACGATGGCACTCACCAgc                   >  2:473319/1‑93 (MQ=255)

                                                                     ggAGTAATATCAAGACGCAGGCGGGCCATGCTGTTTTCGAACAGCGCGATAACCAGGACGCCGACCACCAGTTTTACGATGGCAATCACCAgc                   <  2:3591588/93‑1 (MQ=255)

                                                                     ggAGTAATATCAAGACGCAGGCGGGCCATGCTGTTTTCGAACAGCGCGATAACCAGGACGCCGACCACCAGTTTTACGATGGCAATCACCAgc                   >  1:2111059/1‑93 (MQ=255)

                                                                     ggAGTAATATCAAGACGCAGGCGGGCCATGCTGTTTTCGAACAGCGCGATAACCAGGACGCCGACCACCAGTTTTACGATGGCAATCACCAgc                   >  2:2039099/1‑93 (MQ=255)

                                                                       aGTAATATCAAGACGCAGGCGGGCCATGCTGTTTTCGAACAGCGCGATAACCAGGACGGCGACCACCAGTTTTACGATGGCAATCACCAgcgc                 >  1:828488/1‑93 (MQ=255)

                                                                       aGTAATATCAAGACGCAGGCGGGCCATGCTGTTTTCGAACAGCGCGATAACCAGGACGCCGACCACCAGTTTTACGATGGCAATCACCAgcgc                 <  1:4340441/93‑1 (MQ=255)

                                                                        gTAATATCAAGACGCAGGCGGGCCATGCTGTTTTCGAACAGCGCGATAACCAGGACTCCGACCACCAGATTTACGATGGCAATCACCCgcgca                >  2:2139419/1‑92 (MQ=255)

                                                                        gTAATATCAAGACGCAGGCGGGCCATGCTGTTTTCGAACAGCGCGATAACCAGGACGCCGACCACCAGTTTTACGATGGCAATCACCAGCGcc                >  2:2243347/1‑93 (MQ=255)

                                                                            tatCAAGACGCAGGCGGGCCATGCTGTTTTCGAACAGCGCGATAACCAGGACGCCGACCACCAGTTTTACGATGGCAATCACCAGCGCcagca            >  1:1042959/1‑93 (MQ=255)

                                                                            tatCAAGACGCAGGCGGGCCATGCTGTTTTCGAACAGCGCGATAACCAGGACGCCGACCACCAGTTTTACGATGGCAATCACCAGCGCcagca            >  2:1207112/1‑93 (MQ=255)

                                                                              tCAAGACGCAGGCGGGCCATGCTGTTTTCGAACAGCGCGATAACCAGGACGCCGACCACCAGTTTTACGATGGCAATCACCAGCGCcagcagc          <  2:1675524/93‑1 (MQ=255)

                                                                              tCAAGACGCAGGCGGGCCATGCTGTTTTCGAACAGCGCGATAACCAGGACGCCGACCACCAGTTTTACGATGGCAATCACCAGCGCcagcagc          >  1:3863085/1‑93 (MQ=255)

                                                                               cAAGACGCAGGCGGGCCATGCTGTTTTCGAACAGCGCGATAACCAGGACGCCGACCACCAGTTTTACGATGGCAATCACCAGCGCcagcagca         >  2:4148367/1‑93 (MQ=255)

                                                                                 aGACGCAGGCGGGCCATGCTGTTTTCGAACAGCGCGATAACCAGGACGCCGACCACCAGTTTTACGATGGCAATCACCAGCGCCAGCAGCAGt       >  1:3424914/1‑93 (MQ=255)

                                                                                  gACGCAGGCGGGCCATGCTGTTTTCGAACAGCGCGATAACCAGGACGCCGACCACCAGTTTTACGATGGCAATCACCAGCGCCAGCTGCAGTc      <  2:3902559/93‑1 (MQ=255)

                                                                                  gACGCAGGCGGGCCATGCTGTTTTCGAACAGCGCGATAACCAGGACGCCGACCACCAGTTTTACGATGGCAATCACCAGCGCCAGCAGCAGTc      <  2:3846881/93‑1 (MQ=255)

                                                                                  gACGCAGGCGGGCCATGCTGTTTTCGAACAGCGCGATAACCAGGACGCCGACCACCAGCTTTACGATGGCAATCACCAGCGCCAGCAGCAGTc      >  1:4551549/1‑93 (MQ=255)

                                                                                   aCGCAGGCGGGCCATGCTGTTTTCGAACAGCGCGATAACCAGGACGCCGACCACCAGTTTTACGATGGCAATCACCAGCGCCAGCAGCAGTcc     >  1:2422372/1‑93 (MQ=255)

                                                                                    cGCAGGCGGGCCATGCTGTTTTCGAACAGCGCGATAACCAGGGCGCCGACCACCAGTTTTACGATGGCAATCACCAGCGCCAGCAGCAGTCCa    >  1:2508661/1‑93 (MQ=255)

                                                                                    cGCAGGCGGGCCATGCTGTTTTCGAACAGCGCGATAACCAGGACGCCGACCACCAGTTTTACGATGGCAATCACCAGCGCCAGCAGCAGTCCa    >  2:1973415/1‑93 (MQ=255)

                                                                                    cGCAGGCGGGCCATGCTGTTTTCGAACAGCGCGATAACCAGGACGCCGACCACCAGTTTTACGATGGCAATCACCAGCGCCAGCAGCAGTCCa    <  2:2222870/93‑1 (MQ=255)

                                                                                     gCAGGCGGGCCATGCTGTTTTCGAACAGCGCGATAACCAGGACGCCGACCACCAGTTTTACGATGGCAATCACCAGCGCCAGCAGCAGTCCAc   >  1:219014/1‑93 (MQ=255)

                                                                                      cAGGCGGGCCATGCTGTTTTCGAACAGCGCGATAACCAGGACGCCGACCACCAGTTTTACGATGGCAATCACCAGCGCCAGCAGCAGTCCAcc  <  2:429056/93‑1 (MQ=255)

TTTAATCACGCCGCCAGCAAGGAGACGAACGCTAAAAATGCAAAGCCAAACCCAGCCCAGGTAATGCGCGGAGTAATATCAAGACGCAGACGGGCCATGCTGTTTTCGAACAGCGCGATAACCAGGACGCCGACCACCAGTTTTACGATGGCAATCACCAGCGCCAGCAGCAGTCCACC  >  NC_000913/2844507‑2844685

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 17 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`