BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,933,024	T→C	R256R (CGT→CGC)	fucP →	fucose permease

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,933,024	0	T	C	99.6%	744.5 / NA	233	R256R (CGT→CGC)	fucP	fucose permease
Reads supporting (aligned to +/- strand): ref base T (0/0); major base C (115/117); minor base A (1/0); total (116/117)
Fisher's exact test for biased strand distribution p-value = 4.98e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 245 total aligned reads displayed.

CATGCTGACGAAATTCCCGGCATTGCAGAGTGATAATCACAGTGACGCCAAACAAGGATCGTTCTCCGCATCGCTTTCTCGCCTGGCGCGTATTCGCCACTGGCGCTGGGCGGTATTAGCGCAATTCTGCTATGTCGGCGCACAAACGGCCTGCTGGAGCTATTTGATTCGCTACGCTGT  >  NC_000913/2932934‑2933113

cATGCTGACGAAATTCCCGGCATTGCAGAGTGATAATCACAGTGACGCCAAACAAGGATCGTTCTCCGCATCGCTTTCTCGCCTGGCGCGCAt                                                                                         <  1:4348020/93‑1 (MQ=255)

cATGCTGACGAAATTCCCGGCATTGCAGAGTGATAATCACAGTGACGCCAAACAAGGATCGTTCTCCGCATCGCTTTCTCGCCTGGCGCGCAt                                                                                         <  2:2419428/93‑1 (MQ=255)

 aTGCTGACGAAATTCCCGGCATTGCAGAGTGATAATCACAGTGACGCCAAACAAGGATCGTTCTCCGCATCGCTTTCTCGCCTGGCGCGCAtt                                                                                        <  2:573318/93‑1 (MQ=255)

 aTGCTGACGAAATTCCCGGCATTGCAGAGTGATAATCACAGTGACGCCAAACAAGGATCGTTCTCCGCATCGCTTTCTCGCCTGGCGCGCAtt                                                                                        >  2:138692/1‑93 (MQ=255)

  tGCTGACGAAATTCCCGGCATTGCAGAGTGATAATCACAGTGACGCCAAACAAGGATCGTTCTCCGCATCGCTTTCTCGCCTGGCGCGCATTc                                                                                       <  2:804737/93‑1 (MQ=255)

  tGCTGACGAAATTCCCGGCATTGCAGAGTGATAATCACAGTGACGCCAAACAAGGATCGTTCTCCGCATCGCTTTCTCGCCTGGCGCGCATTc                                                                                       >  1:3371238/1‑93 (MQ=255)

    cTGACGAAATTCCCGGCATTGCAGAGTGATAATCACAGTGACGCCAAACAAGGATCGTTCTCCGCATCGCTTTCTCGCCTGGCGCGCATTCGc                                                                                     >  2:3101859/1‑93 (MQ=255)

     tGACGAAATTCCCGGCATTGCAGAGTGATAATCACAGTGACGCCAAACAAGGATCGTTCTCCGCATCGCTTTCTCGCCTGGCGCNCATTCGcc                                                                                    >  2:3284064/1‑93 (MQ=255)

     tGACGAAATTCCCGGCATTGCAGAGTGATAATCACAGTGACGCCAAACAAGGATCGTTCTCCGCATCGCTTTCTCGCCTGGCGCGCATTCGcc                                                                                    >  1:1185169/1‑93 (MQ=255)

      gACGAAATTCCCGGCATTGCAGAGTGATAATCACAGTGACGCCAAACAAGGATCGTTCTCCGCATCGCTTTCTCGCCTGGCGCGCATTCGCCa                                                                                   >  1:4621401/1‑93 (MQ=255)

      gACGAAATTCCCGGCATTGCAGAGTGATAATCACAGTGACGCCAAACAAGGATCGTTCTCCGCATCGCTTTCTCGCCTGGCGCGCATTCGCCa                                                                                   >  1:1992076/1‑93 (MQ=255)

       aCGAAATTCCCGGCATTGCAGAGTGATAATCACAGTGACGCCAAACAAGGATCGTTCTCCGCATCGCTTTCTCGCCTGGCGCGCATTCGCCAc                                                                                  <  2:2012503/93‑1 (MQ=255)

        cGAAATTCCCGGCATTGCAGAGTGATAATCACAGTGACGCCAAACAAGGATCGTTCTCCGCATCGCTTTCTCGCCTGGCGCGCATTCGCCACt                                                                                 <  2:3327076/93‑1 (MQ=255)

         gAAATTCCCGGCATTGCAGAGTGATAATCACAGTGACGCCAAACAAGGATCGTTCTCCGCATCGCTTTCTCGCCTGGCGCGCATTCGCCACTg                                                                                >  2:1924113/1‑93 (MQ=255)

          aaaTTCCCGGCATTGCAGAGTGATAATCACAGTGGCGCCAAACAAGGATCGTTCTCCGCATCGCTTTCTCGCCTGGCGCGCATTCGCCACTgg                                                                               <  1:527937/93‑1 (MQ=255)

          aaaTTCCCGGCATTGCAGAGTGATAATCACAGTGACGCCAAACAAGGATCGTTCTCCGCATCGCTTTCTCGCCTGGCGCGCATTCGCCACTgg                                                                               >  2:3243094/1‑93 (MQ=255)

           aaTTCCCGGCATTGCAGAGTGATAATCACAGTGACGCCAAACAAGGATCGTTCTCCGCATCGCTTTCTCGCCTGGCGCGCATTCGCCACTGgc                                                                              <  2:4314684/93‑1 (MQ=255)

             ttCCCGGCATTGCAGAGTGATAATCACAGTGACGCCAAACAAGGATCGTTCTCCGCATCGCTTTCTCGCCTGGCGCGCATTCGCCACTGgcgc                                                                            >  1:3086276/1‑93 (MQ=255)

              tCCCGGCATTGCAGAGTGATAATCACAGTGACGCCAAACAAGGATCGTTCTCCGCATCGCTTTCTCGCCTGGCGCGCATTCGCCACTGGCGCt                                                                           >  2:4685784/1‑93 (MQ=255)

                ccGGCATTGCAGAGTGATAATCACAGTGACGCCAATCAAGGATCGTTCTCCGCATCGCTTTCTCGCCTGGCGCGCATTCGCCACTGGCGCTgg                                                                         <  2:3725466/93‑1 (MQ=255)

                ccGGCATTGCAGAGTGATAATCACAGTGACGCCAAACAAGGATCGTTCTCCGCATCGCTTTCTCGCCTGGCGCGCATTCGCCACTGGCGCTgg                                                                         >  2:902095/1‑93 (MQ=255)

                ccGGCATTGCAGAGTGATAATCACAGTGACGCCAAACAAGGATCGTTCTCCGCATCGCTTTCTCGCCTGGCGCGCATTCGCCACTGGCGCTgg                                                                         >  1:1936292/1‑93 (MQ=255)

                  ggCATTGCAGAGTGATAATCACAGTGACGCCAAACAAGGATCGTTCTCCGCATCGCTTTCTCGCCTGGCGCGCATTCGCCACTGGCGCTGGGc                                                                       >  2:1847604/1‑93 (MQ=255)

                  ggCATTGCAGAGTGATAATCACAGTGACGCCAAACAAGGATCGTTCTCCGCATCGCTTTCTCGCCTGGCGCGCATTCGCCACTGGCGCTGGGc                                                                       >  2:1936614/1‑93 (MQ=255)

                  ggCATTGCAGAGTGATAATCACAGTGACGCCAAACAAGGATCGTTCTCCGCATCGCTTTCTCGCCTGGCGCGCATTCGCCACTGGCGCTGGGc                                                                       <  2:3191265/93‑1 (MQ=255)

                   gCATTGCAGAGTGATAATCACAGTGACGCCAAACAAGGATCGTTCTCCGCATCGCTTTCTCGCCTGGCGCGCATTCGCCACTGGCGCTGGGCg                                                                      >  2:2698690/1‑93 (MQ=255)

                      ttGCAGAGTGATAATCCCAGTGACGCCAAACAAGGATCGTTCTCCGCATCGCTTTCTCGCCTGGCGCGCATTCGCCACTGGCGCTGGGCGGTa                                                                   <  2:4378816/93‑1 (MQ=255)

                      ttGCAGAGTGATAATCACAGTGACGCCAAACAAGGATCGTTCTCCGCATCGCTTTCTCGCCTGGCGCGCATTCGCCACTGGCGCTGGGCGGTa                                                                   >  1:4312427/1‑93 (MQ=255)

                         cAGAGTGATAATCACAGTGACGCCAAACAAGGATCGTTCTCCGCATCGCTTTCTCGCCTGGCGCGCATTCGCCACTGGCGCTGGGCGGTATTa                                                                <  1:2792519/93‑1 (MQ=255)

                          agagTGATAATCACAGTGACGCCAAACAAGGATCGTTCTCCGCATCGCTTTCTCGCCTGGCGCGCATTCGCCACTGGCGCTGGGCGGTATTAg                                                               <  2:299909/93‑1 (MQ=255)

                           gagTGATAATCACAGTGACGCCAAACAAGGATCGTTCTCCGCATCGCTTTCTCGCCTGGCGCGCATTCGCCACTGGCGCTGGGCGGTATTAgc                                                              >  1:2018154/1‑93 (MQ=255)

                           gagTGATAATCACAGTGACGCCAAACAAGGATCGTTCTCCGCATCGCTTTCTCGCCTGGCGCGCATTCGCCACTGGCGCTGGGCGGTATTAgc                                                              <  2:2552833/93‑1 (MQ=255)

                               gATAATCACAGTGACGCCAAACAAGGATCGTTCTCCGCATCGCTTTCTCGCCTGGCGCGCATTCGCCACTGGCGCTGGGCGGTATTAGCGCaa                                                          <  2:3430438/93‑1 (MQ=255)

                               gATAATCACAGTGACGCCAAACAAGGATCGTTCTCCGCATCGCTTTCTCGCCTGGCGCGCATTCGCCACTGGCGCTGGGCGGTATTAGCGCaa                                                          <  1:4235371/93‑1 (MQ=255)

                                aTAATCACAGTGACGCCAAACAAGGATCGTTCTCCGCATCGCTTTCTCGCCTGGCGCGCATTCGCCACTGGCGCTGGGCGGTATTAGCGCAAt                                                         >  2:3834775/1‑93 (MQ=255)

                                aTAATCACAGTGACGCCAAACAAGGATCGTTCTCCGCATCGCTTTCTCGCCTGGCGCGCATTCGCCACTGGCGCTGGGCGGTATTAGCGCAAt                                                         >  1:1217340/1‑93 (MQ=255)

                                 tAATCACAGTGACGCCAAACAAGGATCGGTCTCCGCATCGCTTTCTCGCCTGGCGCGCATTCGCCACTGGCGCTGGGCGGTATTAGCGCAAtt                                                        >  1:2489578/1‑93 (MQ=255)

                                     cacaGTGACGCCAAACAAGGATCGTTCTCCGCATCGCTTTCTCGCCTGGCGCGCATTCGCCACTGGCGCTGGGCGGTATTAGCGCAATTCTGc                                                    <  1:1051517/93‑1 (MQ=255)

                                     cacaGTGACGCCAAACAAGGATCGTTCTCCGCATCGCTTTCTCGCCTGGCGCGCATTCGCCACTGGCGCTGGGCGGTATTAGCGCAATTCTGc                                                    >  2:305844/1‑93 (MQ=255)

                                     cacaGTGACGCCAAACAAGGATCGTTCTCCGCATCGCTTTCTCGCCTGGCGCGCATTCGCCACTGGCGCTGGGCGGTATTAGCGCAATTCTGc                                                    >  1:2392062/1‑93 (MQ=255)

                                     cacaGTGACGCCAAACAAGGATCGTTCTCCGCATCGCTTTCTCGCCTGGCGCGCATTCGCCACTGGCGCTGGGCGGTATTAGCGCAATTCTGc                                                    <  1:2307108/93‑1 (MQ=255)

                                      acaGTGACGCCAAACAAGGATCGTTCTCCGCATCGCTTTCTCGCCTGGCGCGCATTCGCCACTGGCGCTGGGCGGTATTAGCGCAATTCTGCt                                                   >  2:693842/1‑93 (MQ=255)

                                       caGTGACGCCAAACAAGGATCGTTCTCCGCATCGCTTTCTCGCCTGGCGCGCATTCGCCACTGGCGCTGGGCGGTATTAGCGCAATTCTGCTa                                                  <  2:2792383/93‑1 (MQ=255)

                                       caGTGACGCCAAACAAGGATCGTTCTCCGCATCGCTTTCTCGCCTGGCGCGCATTCGCCACTGGCGCTGGGCGGTATTAGCGCAATTCTGCTa                                                  <  2:1657000/93‑1 (MQ=255)

                                       caGTGACGCCAAACAAGGATCGTTCTCCGCATCGCTTTCTCGCATGGCGCGCATTCGCCACTGGCGCTGGGCGGTATTAGCGCAATTCTGCTa                                                  <  1:2686089/93‑1 (MQ=255)

                                          tGACGCCAAACAAGGATCGTTCTCCGCATCGCTTTCTCGCCTGGCGCGCATTCGCCACTGGCGCTGGGCGGTATTAGCGCAATTCTGCTATGt                                               <  2:3131783/93‑1 (MQ=255)

                                          tGACGCCAAACAAGGATCGTTCTCCGCATCGCTTTCTCGCCTGGCGCGCATTCGCCACTGGCGCTGGGCGGTATTAGCGCAATTCTGCTATGt                                               >  1:4390318/1‑93 (MQ=255)

                                          tGACGCCAAACAAGGATCGTTCTCCGCATCGCTTTCTCGCCTGGCGCGCATTCGCCACTGGCGCTGGGCGGTATTAGCGCAATTCTGCTATGt                                               <  2:4152162/93‑1 (MQ=255)

                                          tGACGCCAAACAAGGATCGTTCTCCGCATCGCTTTCTCGCCTGGCGCGCATTCGCCACTGGCGCTGGGCGGTATTAGCGCAATTCTGCTATGt                                               <  2:2239052/93‑1 (MQ=255)

                                          tGACACCAAACAAGGATCGTTCTCCTCATCGCTTTCGCGCCTGGCGCGCATTCGCCACTGGCGCTGGGCGGTATTAGCGCAATTCTGCTATGt                                               <  1:3920818/93‑1 (MQ=255)

                                            aCGCCAAACAAGGATCGTTCTCCGCATCGCTTTCTCGCCTGGCGCGCATTCGCCACTGGCGCTGGGCGGTATTAGCGCAATTCTGCTATGTCg                                             >  1:1663332/1‑93 (MQ=255)

                                             cGCCAAACAAGGATCGTTCTCCGCATCGCTTTCTCGCCTGGCGCGCATTCGCCACTGGCGCTGGGCGGTATTAGCGCAATTCTGCTATGTCgg                                            <  1:3440802/93‑1 (MQ=255)

                                                cAAACAAGGATCGTTCTCCACATCGCTTTCTCGCCTGGCGCGCATTCGCCACTGGCGCTGGGCGGTATTAGCGCAATTCTGCTATGTCGgcgc                                         >  2:4526465/1‑93 (MQ=255)

                                                  aaCAAGGATCGTTCTCCGCATCGCTTTCTCGCCTGGCGCGCATTCGCCACTGGCGCTGGGCGGTATTAGCGCAATTCTGCTATGTCGGCGcac                                       >  2:2157865/1‑93 (MQ=255)

                                                  aaCAAGGATCGTTCTCCGCATCGCTTTCTCGCCTGGCGCGCATTCGCCACTGGCGCTGGGCGGTATTAGCGCAATTCTGCTATGTCGGCGcac                                       >  1:3146566/1‑93 (MQ=255)

                                                   aCAAGGATCGTTCTCCGCATCGCTTTCTCGCCTGGCGCGCATTCGCCACTGGCGCTGGGCGGTATTAGCGCAATTCTGCTATGTCGGCGcaca                                      >  1:2098770/1‑93 (MQ=255)

                                                   aCAAGGATCGTTCTCCGCATCGCTTTCTCGCCTGGCGCGCATTCGCCACTGGCGCTGGGCGGTATTAGCGCAATTCTGCTATGTCGGCGcaca                                      >  2:28182/1‑93 (MQ=255)

                                                    cAAGGATCGTTCTCCGCATCGCTTTCTCGCCTGGCGCGCATTCGCCACTGGCGCTGGGCGGTATTAGCGCAATTCTGCTATGTCGGCGCACaa                                     >  2:2712643/1‑93 (MQ=255)

                                                     aaGGATCGTTCTCCGCATCGCTTTCTCGCCTGGCGCGCATTCGCCACTGGCGCTGGGCGGTATTAGCGCAATTCTGCTATGTCGGCGCACaaa                                    <  2:1530843/93‑1 (MQ=255)

                                                     aaGGATCGTTCTCCGCATCGCTTTCTCGCCTGGCGCGCATTCGCCACTGGCGCTGGGCGGTATTAGCGCAATTCTGCTATGTCGGCGCACaaa                                    <  1:79841/93‑1 (MQ=255)

                                                      aGGATCGTTCTCCGCATCGCTTTCTCGCCTGGCGCGCATTCGCCACTGGCGCTGGGCGGTATTAGCGCAATTCTGCTATGTCGGCGCACAAAc                                   >  1:3039372/1‑93 (MQ=255)

                                                      aGGATCGTTCTCCGCATCGCTTTCTCGCCTGGCGCGCATTCGCCACTGGCGCTGGGCGGTATTAGCGCAATTCTGCTATGTCGGCGCACAAAc                                   <  2:4621401/93‑1 (MQ=255)

                                                      aGGATCGTTCTCCGCATCGCTTTCTCGCCTGGCGCGCATTCGCCACTGGCGCTGGGCGGTATTAGCGCAATTCTGCTATGTCGGCGCACAAAc                                   <  1:457737/93‑1 (MQ=255)

                                                      aGGATCGTTCTCCGCATCGCTTTCTCGCCTGGCGCGCATTCACCACTGGCGCTGGGCGGTATTAGCGCAATTCTGCTATGTCGGCGCACAAAc                                   >  1:1761165/1‑93 (MQ=255)

                                                         aTCGTTCTCCGCATCGCTTTCTCGCCTGGCGCGCATTCGCCACTGGCGCTGGGCGGTATTAGCGCAATTCTGCTATGTCGGCGCACAAACGGc                                <  2:2895825/93‑1 (MQ=255)

                                                         aTCGTTCTCCGCATCGCTTTCTCGCCTGGCGCGCATTCGCCACTGGCGCTGGGCGGTATTAGCGCAATTCTGCTATGTCGGCGCACAAACGGc                                <  1:104087/93‑1 (MQ=255)

                                                           cGTTCTCCGCATCGCTTTCTCGCCTGGCGCGCATTCGCCACTGGCGCTGGGCGGTATTAGCGCAATTCTGCTATGTCGGCGCACAAACGGCct                              <  1:1139594/93‑1 (MQ=255)

                                                           cGTTCTCCGCATCGCTTTCTCGCCTGGCGCGCATTCGCCACTGGCGCTGGGCGGTATTAGCGCAATTCTGCTATGTCGGCGCACAAACGGCct                              >  1:4433342/1‑93 (MQ=255)

                                                           cGTTCTCCGCATCGCTTTCTCGCCTGGCGCGCATTCGCCACTGGCGCTGGGCGGTATTAGCGCAATTCTGCTATGTCGGCGCACAAACGGCct                              >  1:159237/1‑93 (MQ=255)

                                                           cGTTCTCCGCATCGCTGTCTCGCCTGGCGCGCATTCGCCACTGGCGCTGGGCGGTATTAGCGCAATTCTGCTATGTCGGCGCACAAACGGCct                              <  1:888975/93‑1 (MQ=255)

                                                           cGTGCTCCGCATCGCTTTCTCGCCTGGCGCGCATTCGCCACTGGCGCTGGGCGGTATTAGCGCAATTCTGCTATGTCGGCGCACAAACGGCct                              <  2:3894640/93‑1 (MQ=255)

                                                            gTTCTCCGCATCGCTTTCTCGCCTGGCGCGAATTCGCCACTGGCGCTGGGCGGTATTAGCGCAATTCTGCTATGTCGGCGCACAAACGGCctg                             >  2:2459632/1‑93 (MQ=255)

                                                              tctcCGCATCGCTTTCTCGCCTGGCGCGCATTCGCCACTGGCGCTGGGCGGTATTAGCGCAATTCTGCTATGTCGGCGCACACACGGCctgct                           >  2:448769/1‑93 (MQ=255)

                                                                tcCGCATCGCTTTCTCGCCTGGCGCGCATTCGCCACTGGCGCTGGGCGGTATTAGCGCAATTCTGCTATGTCGGCGCACAAACGGCCTGCTgg                         <  1:3605809/93‑1 (MQ=255)

                                                                tcCGCATCGCTTTCTCGCCTGGCGCGCATTCGCCACTGGCGCTGGGCGGTATTAGCGCAATTCTGCTATGTCGGCGCACAAACGGCCTGCTgg                         >  1:1369554/1‑93 (MQ=255)

                                                                  ctcgtCGCTTTCTCGCCTGGCGCGCATTCGCCACTGGCGCTGGGCGGTATTAGCGCAATTCTGCTATGTCGGCGCACAAACGGCCTGCTGGAg                       <  1:2933899/89‑1 (MQ=255)

                                                                   gCATCGCTTTCTCGCCTGGCGCGCATTCGCCACTGGCGCTGGGCGGTATTAGCGCAATTCTGCTATGTCGGCGCACAAACGGCCTGCTGGAGc                      >  1:1797213/1‑93 (MQ=255)

                                                                   gCATCGCTTTCTCGCCTGGCGCGCATTCGCCACTGGCGCTGGGCGGTATTAGCGCAATTCTGCTATGTCGGCGCACAAACGGCCTGCTGGAGc                      <  2:148117/93‑1 (MQ=255)

                                                                      tCGCTTTCTCGCCTGGCGCGCATTCGCCACTGGCGCTGGGCGGTATTAGCGCAATTCTGCTATGTCGGCGCACAAACGGCCTGCTGGAGCTAt                   >  2:1200020/1‑93 (MQ=255)

                                                                       cGCTTTCTCGCCTGGCGCGCATTCGCCACTGGCGCTGGGCGGTATTAGCGCAATTCTGCTATGTCGGCGCACAAACGGCCTGCTGGAGCTAtt                  <  1:3966087/93‑1 (MQ=255)

                                                                       cGCTTTCTCGCCTGGCGCGCATTCGCCACTGGCGCTGGGCGGTATTAGCGCAATTCTGCTATGTCGGCGCACAAACGGCCTGCTGGAGCTAtt                  <  1:13004/93‑1 (MQ=255)

                                                                       cGCTTTCTCGCCTGGCGCGCATTCGCCACTGGCGCTGGGCGGTATTAGCGCAATTCTGCTATGTCGGCGCACAAACGGCCTGCTGGAGCTAtt                  <  2:2315863/93‑1 (MQ=255)

                                                                        gCTTTCTCGCCTGGCGCGCATTCGCCACTGGCGCTGGGCGGTATTAGCGCAATTCTGCTATGTCGGCGCACAAACGGCCTGCTGGAGCTAttt                 >  2:350044/1‑93 (MQ=255)

                                                                        gCTTTCTCGCCTGGCGCGCATTCGCCACTGGCGCTGGGCGGTATTAGCGCAATTCTGCTATGTCGGCGCACAAACGGCCTGCTGGAGCTAttt                 <  1:4167810/93‑1 (MQ=255)

                                                                          tttCTCGCCTGGCGCGCATTCGCCACTGGCGCTGGGCGGTATTAGCGCAATTCTGCTATGTCGGCGCACAAACGGCCTGCTGGAGCTATTTGa               <  2:2437199/93‑1 (MQ=255)

                                                                          tttCTCGCCTGGCGCGCATTCGCCACTGGCGCTGGGCGGTATTAGCGCAATTCTGCTATGTCGGCGCACAAACGGCCTGCTGGAGCTATTTGa               >  1:3211896/1‑93 (MQ=255)

                                                                          tttCTCGCCTGGCGCGCATTCGCCACTGGCGCTGGGCGGTATTAGCGCAATTCTGCTATGTCGGCGCACAAACGGCCTGCTGGAGCTATTTGa               >  1:3704782/1‑93 (MQ=255)

                                                                           ttCTCGCCTGGCGCGCATTCGCCACTGGCGCTGGGCGGTATTAGCGCAATTCTGCTATGTCGGCGCACAAACGGCCTGCTGGAGCTATTTGAt              <  2:3608969/93‑1 (MQ=255)

                                                                           ttCTCGCCTGGCGCGCATTCGCCACTGGCGCTGGGCGGTATTAGCGCAATTCTGCTATGTCGGCGCACAAACGGCCTGCTGGAGCTATTTGAt              <  2:4012902/93‑1 (MQ=255)

                                                                             ctcGCCTGGCGCGCATTCGCCACTGGCGCTGGGCGGTATTAGCGCAATTCTGCTATGTCGGCGCACAAACGGCCTGCTGGAGCTATTTGATTc            <  1:4520413/93‑1 (MQ=255)

                                                                                gCCTGGCGCGCATTCGCCACTGGCGCTGGGCGGTATTAGCGCAATTCTGCTATGTCGGCGCACAAACGGCCTGCTGGAGCTATTTGATTCGCt         >  1:3822749/1‑93 (MQ=255)

                                                                                gCCTGGCGCGCATTCGCCACTGGCGCTGGGCGGTATTAGCGCAATTCTGCTATGTCGGCGCACAAACGGCCTGCTGGAGCTATTTGATTCGCt         >  2:4089476/1‑93 (MQ=255)

                                                                                 ccTGGCGCGCATTCGCCACTGGCGCTGGGCGGTATTAGCGCAATTCTGCTATGTCGGCGCACAAACGGCCTGCTGGAGCTATTTGATTCGCTa        <  2:1796097/93‑1 (MQ=255)

                                                                                 ccTGGCGCGCATTCGCCACTGGCGCTGGGCGGTATTAGCGCAATTCTGCTATGTCGGCGCACAAACGGCCTGCTGGAGCTATTTGATTCGCTa        <  2:207105/93‑1 (MQ=255)

                                                                                  cTGGCGCGCATTCGCCACTGGCGCTGGGCGGTATTAGCGCAATTCTGCTATGTCGGCGCACAAACGGCCTGCTGGAGCTATTTGATTCGCTAc       <  1:242741/93‑1 (MQ=255)

                                                                                  cTGGCGCGCATTCGCCACTGGCGCTGGGCGGTATTAGCGCAATTCTGCTATGTCGGCGCACAAACGGCCTGCTGGAGCTATTTGATTCGCTAc       >  2:3946816/1‑93 (MQ=255)

                                                                                   tGGCGCGCATTCGCCACTGGCGCTGGGCGGTATTAGCGCAATTCTGCTATGTCGGCGCACAAACGGCCTGCTGGAGCTATTTGATTCGCTACg      >  1:1313335/1‑93 (MQ=255)

                                                                                    ggCGCGCATTCGCCGCTGGCGCTGGGCGGTATTAGCGCAATTCTGCTATGTCGGCGCACAAACGGCCTGCTGGAGCTATTTGATTCGCTACGc     <  2:1104545/93‑1 (MQ=255)

                                                                                    ggCGCGCATTCGCCACTGGCGCTGGGCGGTATTAGCGCAATTCTGCTATGTCGGCGCGCAAACGGCCTGCTGGAGCTATTTGATTCGCTACGc     <  2:602434/93‑1 (MQ=255)

                                                                                      cgcgCATTCGCCACTGGCGCTGGGCGGTATTAGCGCAATTCTGCTATGTCGGCGCACAAACGGCCTGCTGGAGCTATTTGATTCGCTACGCTg   <  2:3720603/93‑1 (MQ=255)

CATGCTGACGAAATTCCCGGCATTGCAGAGTGATAATCACAGTGACGCCAAACAAGGATCGTTCTCCGCATCGCTTTCTCGCCTGGCGCGTATTCGCCACTGGCGCTGGGCGGTATTAGCGCAATTCTGCTATGTCGGCGCACAAACGGCCTGCTGGAGCTATTTGATTCGCTACGCTGT  >  NC_000913/2932934‑2933113

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`