BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	297,272	A→G	C227R (TGC→CGC)	yagQ ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	297,272	0	A	G	98.6%	442.1 / ‑3.1	145	C227R (TGC→CGC)	yagQ	hypothetical protein
Reads supporting (aligned to +/- strand): ref base A (1/1); new base G (70/73); total (71/74)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.50e-01

Only 100 of 148 total aligned reads displayed.

GGGTTCGATAGCTGCCCAATGCGCCGAGATAAAAGGGTTTTGCTTCTCGCGCGGCCTGCAACACTGGCAGCTCCCGGTTGAGATCATGGCACAGCAAAATGACCGCCGTATCGGTATCGATCTGAGCGCTGGCTGAGGCCGGAAAAAGATCGAAGATATGGCTGTCATAGCCTGTGGC  >  NC_000913/297182‑297359

 ggTTCGATAGCTGCCCAATGCGCCGAGATAAAAGGGTTTTGCTTCTCGCGCGGCCTGCAACACTGGCAGCTCCCGGTTGAGATCATGGCGCAg                                                                                      <  2:2524034/93‑1 (MQ=255)

  gTTCGATAGCTGCCCAATGCGCCGAGATAAAAGGGTTTTGCTTCTCGCGCGGCCTGCAACACTGGCAGCTCCCGGTTGAGATCATGGCGCAGc                                                                                     <  1:4104329/93‑1 (MQ=255)

      gATAGCTGCCCAATGCGCCGAGATAAAAGGGTTTTGCTTCTCGCGCGGCCTGCAACACTGGCAGCTCCCGGTTGAGATCATGGCGCAGCaaaa                                                                                 >  2:3831991/1‑93 (MQ=255)

       aTAGCTGCCCAATGCGCCGAGATAAAAGGGTTTTGCTTCTCGCGCGGCCTGCAACACTGGCAGCTCCCGGTTGAGATCATGGCGCAGCAAAAt                                                                                <  2:1863094/93‑1 (MQ=255)

       aTAGCTGCCCAATGCGCCGAGATAAAAGGGTTTTGCTTCTCGCGCGGCCTGCAACACTGGCAGCTCCCGGTTGAGATCATGGCGCAGCAAAAt                                                                                <  1:4230907/93‑1 (MQ=255)

       aTAGCTGCCCAATGCGCCGAGATAAAAGGGTTTTGCTTCTCGCGCGGCCTGCAACACTGGCAGCTCCCGGTTGAGATCATGGCGCAGCAAAAt                                                                                >  2:4525244/1‑93 (MQ=255)

        tAGCTGCCCAATGCGCCGAGATAAAAGGGTTTTGCTTCTCGCGCGGCCTGCAACACTGGCAGCTCCCGGTTGAGATCATGGCGCAGCAAAATg                                                                               >  2:628269/1‑93 (MQ=255)

          gCTGCCCAATGCGCCGAGATAAAAGGGTTTTGCTTCTCGCGCGGCCTGCAACACTGGCAGCTCCCGGTTGAGATCATGGCGCAGCAAAATGAc                                                                             >  2:1814616/1‑93 (MQ=255)

          gCTGCCCAATGCGCCGAGATAAAAGGGTTTTGCTTCTCGCGCGGCCTGCAACACTGGCAGCTCCCGGTTGAGATCATGGCGCAGCAAAATGAc                                                                             <  1:2938466/93‑1 (MQ=255)

          gCTGCCCAATGCGCCGAGATAAAAGGGTTTTGCTTCTCGCGCGGCCTGCAACACTGGCAGCTCCCGGTTGAGATCATGGCGCAGCAAAATGAc                                                                             <  2:1871242/93‑1 (MQ=255)

               ccAATGCGCCGAGATAAAAGGGTTTTGCTTCTCGCGCGGCCTGCAACACTGGCAGCTCCCGGTTGAGATCATGGCGCAGCAAAATGAccgcct                                                                        >  1:2088572/1‑92 (MQ=255)

               ccAATGCGCCGAGATAAAAGGGTTTTGCTTCTCGCGCGGCCTGCAACACTGGCAGCTCCCGGTTGAGATCATGGCGCAGCAAAATGAccgccg                                                                        <  2:2408156/93‑1 (MQ=255)

                cAATGCGCCGAGATAAAAGGGTTTTGCTTCTCGCGCGGCCTGCAACACTGGCAGCTCCCGGTTGAGATCATGGCGCAGCAAAATGACCGCCgt                                                                       >  2:3588839/1‑93 (MQ=255)

                 aaTGCGCCGAGATAAAAGGGTTTTGCTTCTCGCGCGGCCTGCAACACTGGCAGCTCCCGGTTGAGATCATGGCGCAGCAAAATGACCGCCgta                                                                      <  1:164176/93‑1 (MQ=255)

                      gcCGAGATAAAAGGGTTTTGCTTCTCGCGCGGCCTGCAACACTGGCAGCTCCCGGTTGAGATCATGGCGCAGCAAAATGACCGCCgtatcggt                                                                 >  2:1591230/1‑93 (MQ=255)

                       ccGAGATAAAAGGGTTTTGCTTCTCGCGCGGCCTGCAACACTGGCAGCTCCCGGTTGAGATCATGGCGCAGCAAAATGACCGCCgtatcggta                                                                <  1:3382722/93‑1 (MQ=255)

                        cGAGATAAAAGGGTTTTGCTTCTCGCGCGGCCTGCAACACTGGCAGCTCCCGGTTGAGATCATGGCGCAGCAAAATGACCGCCgtatcggtat                                                               >  2:3081363/1‑93 (MQ=255)

                         gagaTAAAAGGGTTTTGCTTCTCGCGCGGCCTGCAACACTGGCAGCTCCCGGTTGAGATCATGGCGCAGCAAAATGACCGCCgtatcggtatc                                                              >  2:1207062/1‑93 (MQ=255)

                         gagaTAAAAGGGTTTTGCTTCTCGCGCGGCCTGCAACACTGGCAGCTCCCGGTTGAGATCATGGCGCAGCAAAATGACCGCCgtatcggtatc                                                              >  1:1479404/1‑93 (MQ=255)

                          agaTAAAAGGGTTTTGCTTCTCGCGCGGCCTGCAACACTGGCAGCTCCCGGTTGAGATCATGGCGCAGCAAAATGACCGCCgtatcggtatcg                                                             <  2:900327/93‑1 (MQ=255)

                           gaTAAAAGGGTTTTGCTTCTCGCGCGGCCTGCAACACTGGCAGCTCCCGGTTGAGATCATGGCGCAGCAAAATGACCGCCGTATCGGTATCGa                                                            <  1:4577067/93‑1 (MQ=255)

                            aTAAAAGGGTTTTGCTTCTCGCGCGGCCTGCAACACTGGCAGCTCCCGGTTGAGATCATGGCGCAGCAAAATGACCGCCGTATCGGTATCGAt                                                           <  2:907555/93‑1 (MQ=255)

                             tAAAAGGGTTTTGCTTCTCGCGCGGCCTGCAACACTGGCAGCTCCCGGTTGAGATCATGGCGCAGCAAAATGACCGCCGTATCGGTATCGATc                                                          <  1:2685109/93‑1 (MQ=255)

                               aaaGGGTTTTGCTTCTCGCGCGGCCTGCAACACTGGCAGCTCCCGGTTGAGATCATGGCGCAGCAAAATGACCGCCGTATCGGTATCGATCTg                                                        >  2:2084500/1‑93 (MQ=255)

                                aggggTTTTGCTTCTCGCGCGGCCTGCAACACTGGCAGCTCCCGGTTGAGATCATGGCGCAGCAAAATGACCGCCGTATCGGTATCGATCTGa                                                       <  2:3948747/91‑1 (MQ=255)

                                aaGGGTTTTGCTTCTCGCGCGGCCTGCAACACTGGCAGCTCCCGGTTGAGATCATGGCGCAGCAAAATGACCGCCGTATCGGTATCGATCTGa                                                       >  1:4018799/1‑93 (MQ=255)

                                aaGGGTCTTGCTCCTCGCGCGGCCTGCAACACTGGCAGCTCCCGGTTGAGATCATGGCGCAGCAAAATGTCCGCCGTATCGGTATCGATCTGa                                                       <  1:1023553/93‑1 (MQ=255)

                                 aGGGTTTTGCTTCTCGCGCGGCCTGCAACACTGGCAGCTCCCGGTTGAGATCATGGCGCAGCAAAATGACCGCCGTATCGGTATCGATCTGAg                                                      <  1:4134903/93‑1 (MQ=255)

                                  gggTTTTGCTTCTCGCGCGGCCTGCAACACTGGCAGCTCCCGGTTGAGATCATGGCGCAGCAAAATGACCGCCGTATCGGTATCGATCTGAgc                                                     <  2:4252656/93‑1 (MQ=255)

                                   ggTTTTGCTTCTCGCGCGGCCTGCAACACTGGCAGCTCCCGGTTGAGATCATGGCGCAGCAAAATGACCGCCGTATCGGTATCGATCTGAgcg                                                    >  1:1517632/1‑93 (MQ=255)

                                    gTTTTGCTTCTCGCGCGGCCTGCAACACTGGCAGCTCCCGGTTGAGATCATGGCGCAGCAAAATGACCGCCGTATCGGTATCGATCTGAgcgc                                                   <  1:3667515/93‑1 (MQ=255)

                                    gTTTTGCTTCTCGCGCGGCCTGCAACACTGGCAGCTCCCGGTTGAGATCATGGCGCAGCAAAATGACCGCCGTATCGGTATCGATCTGAgcgc                                                   >  1:73903/1‑93 (MQ=255)

                                     ttttGCTTCTCGCGCGGCCTGCAACACTGGCAGCTCCCGGTTGAGATCATGGCGCAGCAAAATGACCGCCGTATCGGTATCGATCTGAGCgct                                                  >  2:3262541/1‑93 (MQ=255)

                                      tttGCTTCTCGCGCGGCCTGCAACACTGGCAGCTCCCGGTTGAGATCATGGCGCAGCAAAATGACCGCCGTATCGGTATCGATCTGAGCgctg                                                 <  2:1435035/93‑1 (MQ=255)

                                      tttGCTTCTCGCGCGGCCTGCAACACTGGCAGCTCCCGGTTGAGATCATGGCGCAGCAAAATGACCGCCGTATCGGTATCGATCTGAGCgctg                                                 >  1:2722119/1‑93 (MQ=255)

                                        tGCTTCTCGCGCGGCCTGCAACACTGGCAGCTCCCGGTTGAGATCATGGCGCAGCAAAATGACCGCCGTATCGGTATCGATCTGAGCgctggc                                               >  1:3411797/1‑93 (MQ=255)

                                        tGCTTCTCGCGCGCCCTGCAACACTGGCAGCTCCCGGTTGAGATCATGGCGCAGCAAAATGACCGCCGTATCGGTATCGATCTGAGCgctggc                                               <  2:3338021/93‑1 (MQ=255)

                                         gCTTCTCGCGCGGCCTGCAACACTGGCAGCTCCCGGTTGAGATCATGGCGCAGCAAAATGACCGCCGTATCGGTATCGATCTGAGCgctggct                                              >  2:971194/1‑93 (MQ=255)

                                         gCTTCTCGCGCGGCCTGCAACACTGGCAGCTCCCGGTTGAGATCATGGCGCAGCAAAATGACCGCCGTATCGGTATCGATCTGAGCgctggct                                              >  1:579536/1‑93 (MQ=255)

                                          cTTCTCGCGCGGCCTGCAACACTGGCAGCTCCCGGTTGAGATCATGGCGCAGCAAAATGACCGCCGTATCGGTATCGATCTGAGCgctggctg                                             >  2:3358188/1‑93 (MQ=255)

                                          cTTCTCGCGCGGCCTGCAACACTGGCAGCTCCCGGTTGAGATCATGGCGCAGCAAAATGACCGCCGTATCGGTATCGATCTGAGCgctggctg                                             >  2:227290/1‑93 (MQ=255)

                                          cTTCTCGCGCGGCCCGCAACACTGGCAGCTCCCGGTTGAGATCATGGCGCAGCAAAATGACCGCCGTATCGGTATCGATCTGAGCgctggctg                                             >  1:3627303/1‑93 (MQ=255)

                                           ttCTCGCGCGGCCTGCAACACTGGCAGCTCCCGGTTGAGATCATGGCGCAGCAAAATGACCGCCGTATCGGTATCGATCTGAGCGCTGGCTGa                                            >  2:2072737/1‑93 (MQ=255)

                                           ttCTCGCGCGGCCTGCAACACTGGCAGCTCCCGGTTGAGATCATGGCGCAGCAAAATGACCGCCGTATCGGTATCGATCTGAGCGCTGGCTGa                                            <  2:3422974/93‑1 (MQ=255)

                                           ttCTCGCGCGGCCTGCAACACTGGCAGCTCCCGGTTGAGATCATGGCGCAGCAAAATGACCGCCGTATCGGTATCGATCTGAGCGCTGGCTGa                                            >  1:3027289/1‑93 (MQ=255)

                                           ttCTCGCGCGGCCTGCAACACTGGCAGCTCCCGGTTGAGATCATGGCGCAGCAAAATGACCGCCGTATCGGTATCGATCTGAGCGCTGGCTGa                                            <  1:339400/93‑1 (MQ=255)

                                            tctcGCGCGGCCTGCAACACTGGCAGCTCCCGGTTGAGATCATGGCGCAGCAAAATGACCGCCGTATCGGTATCGATCTGAGCGCTGGCTGAg                                           <  2:1997961/93‑1 (MQ=255)

                                            tctcGCGCGGCCTGCAACACTGGCAGCTCCCGGTTGAGATCATGGCGCAGCAAAATGACCGCCGTATCGGTATCGATCTGAGCGCTGGCTGAg                                           <  1:2485077/93‑1 (MQ=255)

                                            tctcGCGCGGCCTGCAACACTGGCAGCTCCCGGTTGAGATCATGGCGCAGCAAAATGACCGCCGTATCGGTATCGATCTGAGCGCTGGCTGAg                                           >  2:1743039/1‑93 (MQ=255)

                                            tctcGCGCGGCCTGCAACACTGGCAGCTCCCGGTTGAGATCATGGCGCAGCAAAATGACCGCCGTATCGGTATCGATCTGAGCGCTGGCTGAg                                           >  1:4148152/1‑93 (MQ=255)

                                            tctcGCGCGGCCTGCAACACTGGCAGCTCCCGGTTGAGATCATGGCGCAGCAAAATGACCGCCGTATCGGTATCGATCTGAGCGCTGGCTGAg                                           >  2:329075/1‑93 (MQ=255)

                                             ctcGCGCTGCCGGCAACACTGGCAGCTCCCGGTTGAGAGCATGGCGCAGCAAAATGACCGCCGTATCGGTATCGATCTGAGCGCTGGCTGAgg                                          <  2:1738710/93‑1 (MQ=255)

                                              tcGCGCGGCCTGCAACACTGGCAGCTCCCGGTTGAGATCATGGCGCAGCAAAATGACCGCCGTATCGGTATCGATCTGAGCGCTGGCTGAGGc                                         >  1:4512914/1‑93 (MQ=255)

                                              tcGCGCGGCCTGCAACACTGGCAGCTCCCGGTTGAGATCATGGCGCAGCAAAATGACCGCCGTATCGGTATCGATCTGAGCGCTGGCTGAGGc                                         >  1:3196882/1‑93 (MQ=255)

                                                gcgcgGCCTGCAACACTGGCAGCTCCCGGTTGAGATCATGGCGCAGCAAAATGCCCGCCGTATCGGTATCGATCTGAGCGCTGGCTGAGGCCg                                       <  2:82919/93‑1 (MQ=255)

                                                gcgcgGCCTGCAACACTGGCAGCTCCCGGTTGAGATCATGGCGCAGCAAAATGACCGCCGTATCGGTATCGATCTGAGCGCTGGCTGAGGCCg                                       <  2:2202502/93‑1 (MQ=255)

                                                 ctcgGCCTGCAACACTGGCAGCTCCCGGTTGAGATCATGGCGCAGCAAAATGACCGCCGTATCGGTATCGATCTGAGCGCTGGCTGAGGCCgg                                      <  2:3928045/91‑1 (MQ=255)

                                                  gcgGCCTGCAACACTGGCGGCTCCCGGTTGAGATCATGGCGCAGCAAAATGACCGCCGTATCGGTATCGATCTGAGCGCTGGCTGAGGCCGGa                                     <  2:4594950/93‑1 (MQ=255)

                                                  gcgGCCTGCAACACTGGCAGCTCCCGGTTGAGATCATGGCGCAGCAAAATGACCGCCGTATCGGTATCGATCTGAGCGCTGGCTGAGGCCGGa                                     >  1:4382856/1‑93 (MQ=255)

                                                   cgGCCTCCAACACTGGCAGCTCCCGGTTGAGATCATGGCGCAGCAAAATGACCGCCGTATCGGTATCGATCTGAGCGCTGGCTGAGGCCGGaa                                    <  2:1397309/93‑1 (MQ=255)

                                                    ggCCTGCAACACTGGCAGCTCCCGGTTGAGATCATGGCGCAGCAAAATGACCGCCGTATCGGTATCGATCTGAGCGCTGGCTGAGGCCGGaaa                                   <  1:1014097/93‑1 (MQ=255)

                                                    ggCCTGCAACACTGGCAGCTCCCGGTTGAGATCATGGCGCAGCAAAATGACCGCCGTATCGGTATCGATCTGAGCGCTGGCTGAGGCCGGaaa                                   >  2:3787898/1‑93 (MQ=255)

                                                      ccTGCAACACTGGCAGCTCCCGGTTGAGATCATGGCGCAGCAAAATGATCGCCGTATCGGTATCGATCTGAGCGCTGGCCGCGGCCGGaaaaa                                 >  2:3787379/1‑93 (MQ=255)

                                                      ccTGCAACACTGGCAGCTCCCGGTTGAGATCATGGCGCAGCAAAATGACCGCCGTATCGGTATCGATCTGAGCGCTGGCTGAGGCCGGaaaaa                                 <  2:848095/93‑1 (MQ=255)

                                                        tGCAACACTGGCAGCTCCCGGTTGAGATCATGGCGCAGCAAAATGACCGCCGTATCGGTATCGATCTGAGCGCTGGCTGAGGCCGGAAAAAGa                               >  1:2564643/1‑93 (MQ=255)

                                                        tGCAACACTGGCAGCTCCCGGTTGAGATCATGGCGCAGCAAAATGACCGCCGTATCGGTATCGATCTGAGCGCTGGCTGAGGCCGGAAAAAGa                               >  2:2402492/1‑93 (MQ=255)

                                                         gCAACACTGGCAGCTCCCGGTTGAGATCATGGCGCAGCAAAATGACCGCCGTATCGGTATCGATCTGAGCGCTGGCTGAGGCCGGAAAAAGAt                              <  2:1774374/93‑1 (MQ=255)

                                                         gCAACACTGGCAGCTCCCGGTTGAGATCATGGCGCAGCAAAATGACCGCCGTATCGGTATCGATCTGAGCGCTGGCTGAGGCCGGAAAAAGAt                              <  2:3978737/93‑1 (MQ=255)

                                                         gCAACACTGGCAGCTCCCGGTTGAGATCATGGCGCAGCAAAATGACCGCCGTATCGGTATCGATCTGAGCGCTGGCTGAGGCCGGAAAAAGAt                              <  2:1479404/93‑1 (MQ=255)

                                                         gCAACACTGGCAGCTCCCGGTTGAGATCATGGCGCAGCAAAATGACCGCCGTATCGGTATCGATCTGAGCGCTGGCTGAGGCCGGAAAAAGAt                              <  1:1099410/93‑1 (MQ=255)

                                                           aaCACTGGCAGCTCCCGGTTGAGATCATGGCGCAGCAAAATGACCGCCGTATCGGTATCGATCTGAGCGCTGGCTGAGGCCGGAAAAAGATCg                            <  1:2373136/93‑1 (MQ=255)

                                                           aaCACTGGCAGCTCCCGGTTGAGATCATGGCGCAGCAAAATGACCGCCGTATCGGTATCGATCTGAGCGCTGGCTGAGGCCGGAAAAAGATCg                            >  1:3845147/1‑93 (MQ=255)

                                                            acacTGGCAGCTCCCGGTTGAGATCATGGCGCAGCAAAATGACCGCCGTATCGGTATCGATCTGAGCGCTGGCTGAGGCCGGAAAAAGATCGa                           >  1:4467889/1‑93 (MQ=255)

                                                              acTGGCAGCTCCCGGTTGAGATCATGGCGCAGCAAAATGACCGCCGTATCGGTATCGATCTGAGCGCTGGCTGAGGCCGGAAAAAGATCGAAg                         >  1:2862490/1‑93 (MQ=255)

                                                              acTGGCAGCTCCCGGTTGAGATCATGGCGCAGCAAAATGACCGCCGTATCGGTATCGATCTGAGCGCTGGCTGAGGCCGGAAAAAGATCGAAg                         <  1:1265950/93‑1 (MQ=255)

                                                                tGGGAGCTCCCGGTTGAGATCATGGCGAAGCAAAATGACCGCCGTATCGGTATCGATCTGAGCGCTGGCTGAGGCCGGAAAAAGATCGAAGat                       <  1:582515/93‑1 (MQ=255)

                                                                 ggCAGCTCCCGGTTGAGATCATGGCGCAGCAAAATGACCGCCGTATCGGTATCGATCTGAGCGCTGGCTGAGGCCGGAAAAAGATCGAAGata                      <  1:2300872/93‑1 (MQ=255)

                                                                 ggCAGCTCCCGGTTGAGATCATGGCGCAGCAAAATGACCGCCGTATCGGTATCGATCTGAGCGCTGGCTGAGGCCGGAAAAAGATCGAAGata                      <  2:2880220/93‑1 (MQ=255)

                                                                 ggCAGCTCCCGGTTGAGATCATGGCGCAGCAAAATGACCGCCGTATCGGTATCGATCTGAGCGCTGGCTGAGGCCGGAAAAAGATCGAAGata                      <  1:2776895/93‑1 (MQ=255)

                                                                  gCAGCTCCCGCTTGAGATCATGGCGCAGCAAAATGACCGCCGTATCGGTATCGATCTGAGCGCTGGCTGAGGCCGGAAAAAGATCGAAGatat                     <  2:2830829/93‑1 (MQ=255)

                                                                   cAGCTCCCGGTTGAGATCATGGCGCAGCAAAATGTCCGCCGTATCGGTATCGATCTGAGCGCTGGCTGAGGCCGGAAAAAGATCGAAGATATg                    <  2:1239228/93‑1 (MQ=255)

                                                                     gCTCCCGGTTGAGATCATGGCGCAGCAAAATGCCCGCCGTATCGGTATCGATCTGAGCGCTGGCTGAGGCCGGAAAAAGATCGAAGATATGGc                  <  2:261542/93‑1 (MQ=255)

                                                                     gCTCCCGGTTGAGATCATGGCGCAGCAAAATGACCGCCGTATCGGTATCGATCTGAGCGCTGGCTGAGGCCGGAAAAAGATCGAAGATATGGc                  >  2:4347238/1‑93 (MQ=255)

                                                                     gCTCCCGGTTGAGATCATGGCGCAGCAAAATGACCGCCGTATCGGTATCGATCTGAGCGCTGGCTGAGGCCGGAAAAAGATCGAAGATATGGc                  >  2:4291445/1‑93 (MQ=255)

                                                                     gCTCCCGGTTGAGATCATGGCGCAGCAAAATGACCGCCGTATCGGTATCGATCTGAGCGCTGGCTGAGGCCGGAAAAAGATCGAAGATATGGc                  <  1:1171058/93‑1 (MQ=255)

                                                                         ccGGTTGAGATCATGGCGCAGCAAAATGACCGCCGTATCGGTATCGATCTGAGCGCTGGCTGAGGCCGGAAAAAGATCGAAGATATGGCTGta              >  1:2377993/1‑92 (MQ=255)

                                                                          cGGTTGAGATCATGGCGCAGCAAAATGACCGCCGTATCGGTATCGATCTGAGCGCTGGCTGAGGCCGGAAAAAGATCGAAGATATGGCTGTCa             <  1:2003183/93‑1 (MQ=255)

                                                                          cGGTTGAGATCATGGCGCAGCAAAATGACCGCCGTATCGGTATCGATCTGAGCGCTGGCTGAGGCCGGAAAAAGATCGAAGATATGGCTGTCa             >  1:3859662/1‑93 (MQ=255)

                                                                           ggTTGAGATCATGGCGCAGCAAAATGACCGCCGTATCGGTATCGATCTGAGCGCTGGCTGAGGCCGGAAAAAGATCGAAGATATGGCTGTCAt            <  2:441926/93‑1 (MQ=255)

                                                                           ggTTGAGATCATGGCGCAGCAAAATGACCGCCGTATCGGTATCGATCTGAGCGCTGGCTGAGGCCGGAAAAAGATCGAAGATATGGCTGTCAt            <  1:1930407/93‑1 (MQ=255)

                                                                           ggTTGAGATCATGGCGCAGCAAAATGACCGCCGTATCGGTATCGATCTGAGCGCTGGCTGAGGCCGGAAAAAGATCGAAGATATGGCTGTCAt            >  1:1694681/1‑93 (MQ=255)

                                                                            gTTGAGATCATGGCGCAGCAAAATGACCGCCGTATCGGTATCGATCTGAGCGCTGGCTGAGGCCGGAAAAAGATCGAAGATATGGCTGTCATa           <  1:2457104/93‑1 (MQ=255)

                                                                            gTTGAGATCATGGCGCAGCAAAATGACCGCCGTATCGGTATCGATCTGAGCGCTGGCTGAGGCCGGAAAAAGATCGAAGATATGGCTGTCATa           >  1:2830305/1‑93 (MQ=255)

                                                                               gagaTCATGGCGCAGCAAAATGACCGCCGTATCGGTATCGATCTGAGCGCTGGCTGAGGCCGGAAAAAGATCGAAGATATGGCTGTCATAGcc        >  2:2013723/1‑93 (MQ=255)

                                                                               gagaTCATGGCGCAGCAAAATGACCGCCGTATCGGTATCGATCTGAGCGCTGGCTGAGGCCGGAAAAAGATCGAAGATATGGCTGGCATAGcc        >  1:328947/1‑93 (MQ=255)

                                                                                 gaTCATGGCGCAGCAAAATGACCGCCGTATCGGTATCGATCTGAGCGCTGGCTGAGGCCGGAAAAAGATCGAAGATATGGCTGTCATAGCCtg      <  2:1527824/93‑1 (MQ=255)

                                                                                  aTCATGGCGCAGCAAAATGACCGCCGTATCGGTATCGATCTGAGCGCTGGCTGAGGCCGGAAAAAGATCGAAGATATGGCTGTCATAGCCtgt     >  2:510452/1‑93 (MQ=255)

                                                                                  aTCATGGCGCAGCAAAATGACCGCCGTATCGGTATCGATCTGAGCGCTGGCTGAGGCCGGAAAAAGATCGAAGATATGGCTGTCATAGCCtgt     <  1:4577242/93‑1 (MQ=255)

                                                                                  aTCATGGCGCAGCAAAATGACCGCCGTATCGGTATCGATCTGAGCGCTGGCTGAGGCCGGAAAAAGATCGAAGATATGGCTGTCATAGCCtgt     <  1:813197/93‑1 (MQ=255)

                                                                                     aTGGCGCAGCAAAATGACCGCCGTATCGGTATCGATCTGAGCGCTGGCTGAGGCCGGAAAAAGATCGAAGATATGGCTGTCATAGCCTGTGgc  <  2:980725/93‑1 (MQ=255)

GGGTTCGATAGCTGCCCAATGCGCCGAGATAAAAGGGTTTTGCTTCTCGCGCGGCCTGCAACACTGGCAGCTCCCGGTTGAGATCATGGCACAGCAAAATGACCGCCGTATCGGTATCGATCTGAGCGCTGGCTGAGGCCGGAAAAAGATCGAAGATATGGCTGTCATAGCCTGTGGC  >  NC_000913/297182‑297359

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 18 ≤` `ATCG/ATCG` `< 26 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`