BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,026,063	A→G	D129G (GAC→GGC)	ygfR →	putative oxidoreductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,026,063	0	A	G	99.4%	551.1 / ‑4.4	173	D129G (GAC→GGC)	ygfR	putative oxidoreductase
Reads supporting (aligned to +/- strand): ref base A (0/0); major base G (90/82); minor base T (0/1); total (90/83)
Fisher's exact test for biased strand distribution p-value = 4.80e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.19e-01

Only 100 of 176 total aligned reads displayed.

CTACTGGCACCATCCGTGCCGTCGCCGGTCAGGCGAATTTGTTGGATAAAGACAACCAGATCATCAACGGCGGCAAAGCCCTGACCAGTGACTCAGTAAGTTCAATATTCTCCGGCCTGGTGGGCGCAGCGCCCGCAGCGGTTTATATCGAATCAGCGGCAGGAACCGCCGCCGGGGGT  >  NC_000913/3025973‑3026151

ctccTGGCACCATCCGTGCCGTCGCCGGTCAGGCGAATTTGTTGGATAAAGACACCCAGATCATCAACGGCGGCAAAGCCCTGACCAGTGGCt                                                                                        <  2:1729931/90‑1 (MQ=255)

   cTGGCACCATCCGTGCCGTCGCCGGTCAGGCGAATTTGTTGGATAAAGACAACCAGATCATCAACGGCGGCAAAGCCCTGACCAGTGGCTCAg                                                                                     >  2:669405/1‑93 (MQ=255)

   cTGGCACCATCCGTGCCGTCGCCGGTCAGGCGAATTTGTTGGATAAAGACAACCAGATCATCAACGGCGGCAAAGCCCTGACCAGTGGCTCAg                                                                                     >  2:4493845/1‑93 (MQ=255)

   cTGGCACCATCCGTGCCGTCGCCGGTCAGGCGAATTTGTTGGATAAAGACAACCAGATCATCAACGGCGGCAAAGCCCTGACCAGTGGCTCAg                                                                                     <  1:2227057/93‑1 (MQ=255)

      gCACCATCCGTGCCGTCGCCGGTCAGGCGAATTTGTTGGATAAAGACAACCAGATCATCAACGGCGGCAAAGCCCTGACCAGTGGCTCAGTaa                                                                                  >  2:4211804/1‑93 (MQ=255)

        aCCATCCGGGCCGTCGCCGGTCAGGCGAATTTGTTGGATAAACACAACCAGATCATCAACGGCGGCAAAGCCCTGACCAGAGCCTCAGGAAGt                                                                                >  1:1447587/1‑93 (MQ=255)

         ccATCCGTGCCGTCGCCGGTCAGGCGAATTTGTTGGATAAAGACAACCAGATCATCAACGGCGGCAAAGCCCTGACCAGTGGCTCAGTAAGtt                                                                               <  2:3133025/93‑1 (MQ=255)

         ccATCCGTGCCGTCGCCGGTCAGGCGAATTTGTTGGATAAAGACAACCAGATCATCAACGGCGGCAAAGCCCTGACCAGTGGCTCAGTAAGtt                                                                               >  2:1285205/1‑93 (MQ=255)

         ccATCCGTGCCGTCGCCGGTCAGGCGAATTTGTTGGATAAAGACAACCAGATCATCAACGGCGGCAAAGCCCTGACCAGTGGCTCAGTAAGtt                                                                               >  1:1706564/1‑93 (MQ=255)

         ccATCCGTGCCGTCGCCGGTCAGGCGAATTTGTTGGATAAAGACAACCAGATCATCAACGGCGGCAAAGCCCTGACCAGTGGCTCAGTAAGtt                                                                               >  1:4221902/1‑93 (MQ=255)

           aTCCGTGCCGTCGCCGGTCAGGCGAATTTGTTGGATAAAGACAACCAGATCATCAACGGCGGCAAAGCCCTGACCAGTGGCTCAGTAAGTTCa                                                                             >  2:4343916/1‑93 (MQ=255)

            tCCGTGCCGTCGCCGGTCAGGCGAATTTGTTGGATAAAGACAACCAGATCATCAACGGCGGCAAAGCCCTGACCAGTGGCTCAGTAAGTTCaa                                                                            <  1:455128/93‑1 (MQ=255)

            tCCGTGCCGTCGCCGGTCAGGCGAATTTGTTGGATAAAGACAACCAGATCATCAACGGCGGCAAAGCCCTGACCAGTGGCTCAGTAAGTTCaa                                                                            <  2:2052444/93‑1 (MQ=255)

            tCCGTGCCGTCGCCGGTCAGGCGAATTTGTTGGATAAAGACAACCAGATCATCAACGGCGGCAAAGCCCTGACCAGTGGCTCAGGAAGTTCaa                                                                            >  2:2054597/1‑93 (MQ=255)

             ccGTGCCGTCGCCGGTCAGGCGAATTTGTTGGATAAAGACAACCAGATCATCAACGGCGGCAAAGCCCTGACCAGTGGCTCAGTAAGTTCAat                                                                           <  2:399721/93‑1 (MQ=255)

             ccGTGCCGTCGCCGGTCAGGCGAATTTGTTGGATAAAGACAACCAGATCATCAACGGCGGCAAAGCCCTGACCAGTGGCTCAGTAAGTTCAat                                                                           <  1:3671445/93‑1 (MQ=255)

              cGTGCCGTCGCCGGTCAGGCGAATTTGTTGGATAAAGACAACCAGATCATCAACGGCGGCAAAGCCCTGACCAGTGGCTCAGTAAGTTCAata                                                                          <  2:1049105/93‑1 (MQ=255)

              cGTGCCGTCGCCGGTCAGGCGAATTTGTTGGATAAAGACAACCAGATCATCAACGGCGGCAAAGCCCTGACCAGTGGCTCAGTAAGTTCAata                                                                          <  2:3667302/93‑1 (MQ=255)

              cGTGCCGTCGCCGGTCAGGCGAATTTGTTGGATAAAGACAACCAGATCATCAACGGCGGCAAAGCCCTGACCAGTGGCTCAGTAAGTTCAata                                                                          <  2:1510951/93‑1 (MQ=255)

               gTGCCGTCGCCGGTCAGGCGAATTTGTTGGATAAAGACAACCAGATCATCAACGGCGGCAAAGCCCTGACCAGTGGCTCAGTAAGTTCAatat                                                                         <  1:4279348/93‑1 (MQ=255)

               gTGCCGTCGCCGGTCAGGCGAATTTGTTGGATAAAGACAACCAGATCATCAACGGCGGCAAAGCCCTGACCAGTGGCTCAGTAAGTTCAatat                                                                         <  1:1999536/93‑1 (MQ=255)

               gTGCCGTCGCCGGTCAGGCGAATTTGTTGGATAAAGACAACCAGATCATCAACGGCGGCAAAGCCCTGACCAGTGGCTCAGTAAGTTCAatat                                                                         >  2:3640346/1‑93 (MQ=255)

               gTGCCGTCGCCGGTCAGGCGAATTTGTTGGATAAAGACAACCAGATCATCAACGGCGGCAAAGCCCTGACCAGTGGCTCAGTAAGTTCAatat                                                                         <  2:1572465/93‑1 (MQ=255)

                tGCCGTCGCCGGTCAGGCGAATTTGTTGGATAAAGACAACCAGATCATCAACGGCGGCAAAGCCCTGACCAGTGGCTCAGTAAGTTCAATAtt                                                                        >  1:2586860/1‑93 (MQ=255)

                tGCCGTCGCCGGTCAGGCGAATTTGTTGGATAAAGACAACCAGATCATCAACGGCGGCAAAGCCCTGACCAGTGGCTCAGTAAGATCAATAtt                                                                        >  1:1119848/1‑93 (MQ=255)

                  ccGTCGCCGGTCAGGCGAATTTGTTGGATAAAGACAACCAGATCATCAACGGCGGCAAAGCCCTGACCAGTGGCTCAGTAAGTTCAATATtct                                                                      <  1:674850/93‑1 (MQ=255)

                   cGTCGCCGGTCAGGCGAATTTGTTGGATAAAGACAACCAGATCATCAACGGCGGCAAAGCCCTGACCAGTGGCTCAGTAAGTTCAATATtctc                                                                     <  2:1532218/93‑1 (MQ=255)

                   cGTCGCCGGTCAGGCGAATTTGTTGGATAAAGACAACCAGATCATCAACGGCGGCAAAGCCCTGACCAGTGGCTCAGTAAGTTCAATATtctc                                                                     <  2:224628/93‑1 (MQ=255)

                    gTCGCCGGTCAGGCGAATTTGTTGGATAAAGACAACCAGATCATCAACGGCGGCAAAGCCCTGACCAGTGGCTCAGTAAGTTCAATATTCTcc                                                                    <  2:1669548/93‑1 (MQ=255)

                     tCGCCGGTCAGGCGAATTTGTTGGATAAAGACAACCAGATCATCAACGGCGGCAAAGCCCTGACCAGTGGCTCAGTAAGTTCAATATTCTCCg                                                                   <  1:1320643/93‑1 (MQ=255)

                        ccGGTCAGGCGAATTTGTTGGATAAAGACAACCAGATCATCAACGGCGGCAAAGCCCTGACCAGTGGCTCAGTAAGTTCAATATTCTCCGGcc                                                                <  2:641148/93‑1 (MQ=255)

                         cGGTCAGGCGAATTTGTTGGATAAAGACAACCAGATCATCAACGGCGGCAAAGCCCTGACCAGTGGCTCAGTAAGTTCAATATTCTCCGGCCt                                                               >  2:281764/1‑93 (MQ=255)

                         cGGTCAGGCGAATTTGTTGGATAAAGACAACCAGATCATCAACGGCGGCAAAGCCCTGACCAGTGGCTCAGTAAGCTCAATATTCTCCGGCCt                                                               >  1:509330/1‑93 (MQ=255)

                          ggTCAGGCGAATTTGTTGGATAAAGACAACCAGATCATCAACGGCGGCAAAGCCCTGACCAGTGGCTCAGTAAGTTCAATATTCTCCGGCCtg                                                              >  1:2817685/1‑93 (MQ=255)

                           gTCAGGCGAATTTGTTGGATAAAGACAACCAGATCATCAACGGCGGCAAAGCCCTGACCAGGGGCTCAGTAAGTTCAATATTCTCCGGCCtgg                                                             >  1:3977589/1‑93 (MQ=255)

                            tCAGGCGAATTTGTTGGATAAAGACAACCAGATCATCAACGGCGGCAAAGCCCTGACCAGTGGCTCAGTAAGTTCAATATTCTCCGGCCtggt                                                            <  1:3406070/93‑1 (MQ=255)

                            tCAGGCGAATTTGTTGGATAAAGACAACCAGATCATCAACGGCGGCAAAGCCCTGACCAGTGGCTCAGTAAGTTCAATATTCTCCGGCCtggg                                                            >  1:1544871/1‑92 (MQ=255)

                              aGGCGAATTTGTTGGATAAAGACAACCAGATCATCAACGGCTGCAAAGCCCTGACCAGTGGCTCAGTAAGTTCAATATTCTCCGGCCtggtgg                                                          >  2:4181457/1‑93 (MQ=255)

                               ggCGAATTTGTTGGATAAAGACAACCAGATCATCAACGGCGGCAAAGCCCTGACCAGTGGCTCAGTAAGTTCAATATTCTCCGGCCTGGTggg                                                         >  2:2949223/1‑93 (MQ=255)

                                 cGAATTTGTTGGATAAAGACAACCAGATCATCAACGGCGGCAAAGCCCTGACCAGTGGCTCAGTAAGTTCAATATTCTCCGACCTGGTGGgcg                                                       >  1:1547976/1‑93 (MQ=255)

                                   aaTTTGTTGGATAAAGACAACCAGATCATCAACGGCGGCAAAGCCCTGACCAGTGGCTCAGTAAGTTCAATATTCTCCGGCCTGGTGGGCGCa                                                     <  1:1269756/93‑1 (MQ=255)

                                    aTTGGTTGGATAAAGACAACCAGTTCATCAACGGCGGCAAAGCCCTGACCAGTGGCTCAGTAAGTTCAATATTCTCCGGCCTGGTGGGCGCAg                                                    <  2:1335833/93‑1 (MQ=255)

                                     tttGTTGGATAAAGACAACCAGATCATCAACGGCGGCAAAGCCCTGACCAGTGGCTCAGTAAGTTCAATATTCTCCGGCCTGGTGGGCGCAgc                                                   <  1:2180034/93‑1 (MQ=255)

                                     tttGTTGGATAAAGACAACCAGATCATCAACGGCGGCAAAGCCCTGACCAGTGGCTCAGTAAGTTCAATATTCTCCGGCCTGGTGGGCGCAgc                                                   <  2:3451811/93‑1 (MQ=255)

                                      ttgttgGATAAAGACAACCAGATCATCAACGGCGGCAAAGCCCTGACCAGTGGCTCAGTAAGTTCAATATTCTCCGGCCTGGTGGGCGCAgcg                                                  >  2:2552543/1‑93 (MQ=255)

                                      ttgttgGATAAAGACAACCAGATCATCAACGGCGGCAAAGCCCTGACCAGTGGCTCAGTAAGTTCAATATTCTCCGGCCTGGTGGGCGCAgcg                                                  >  2:372291/1‑93 (MQ=255)

                                      ttgttgGATAAAGACAACCAGATCATCAACGGCGGCAAAGCCCTGACCAGTGGCTCAGTAAGTTCAATATTCTCCGGCCTGGTGGGCGCAgcg                                                  >  2:875024/1‑93 (MQ=255)

                                        gttgGATAAAGACAACCAGATCATCAACGGCGGCAAAGCCCTGACCAGTGGCTCAGTAAGTTCAATATTCTCCGGCCTGGTGGGCGCAGCGcc                                                >  1:2689003/1‑93 (MQ=255)

                                         ttgGATAAAGACAACCAGATCATCAACGGCGGCAAAGCCCTGACCAGTGGCTCAGTAAGTTCAATATTCTCCGGCCTGGTGGGCGCAGCGccc                                               >  1:312227/1‑93 (MQ=255)

                                          tgGATAAAGACAACCAGATCATCAACGGAGGCAAAGCCCTGACCAGTGGCTCAGTAAGTTCAATATTCTCCGGCCTGTTGTGCGCAGCGCCCg                                              >  2:2327652/1‑93 (MQ=255)

                                           ggATAAAGACAACCAGATCATCAACGGCGGCAAAGCCCTGACCAGTGGCTCAGTAAGTTCAATATTCTCCGGCCTGGTGGGCGCAGCGCCCGc                                             >  2:4457796/1‑93 (MQ=255)

                                            gATAAAGACAACCAGATCATCAACGGCGGCAAAGCCCTGACCAGTGGCTCAGTAAGTTCAATATTCTCCGGCCTGGTGGGCGCAGCGCCCGCa                                            <  1:114459/93‑1 (MQ=255)

                                             aTAAAGACAACCAGATCATCAACTGCGGCAAAGCCCTGACCAGTGGCTCAGTAAGTTCAATATTCTCCGGCCTGGTGGGCGCAGCGCCCGCAg                                           <  1:938279/93‑1 (MQ=255)

                                              tAAAGACAACCAGATCATCAACGGCGGCAAAGCCCTGACCAGTGTCTCAGTAAGTTCAATATTCTCCGGCCTGGTGGGCGCAGCGCCCGCAGc                                          <  1:1420907/93‑1 (MQ=255)

                                              tAAAGACAACCAGATCATCAACGGCGGCAAAGCCCTGACCAGTGGCTCAGTAAGTTCAATATTCTCCGGCCTGGTGGGCGCAGCGCCCGCAGc                                          >  2:1215533/1‑93 (MQ=255)

                                              tAAAGACAACCAGATCATCAACGGCGGCAAAGCCCTGACCAGTGGCTCAGTAAGTTCAATATTCTCCGGCCTGGTGGGCGCAGCGCCCGCAGc                                          <  2:2748906/93‑1 (MQ=255)

                                                 aGACAACCAGATCATCAACGGCGGCAAAGCCCTGACCAGTGGCTCAGTAAGTTCAATATTCTCCGGCCTGGTGGGCGCAGCGCCCGCAGCGGt                                       >  2:2388088/1‑93 (MQ=255)

                                                  gACAACCAGATCATCAACGGCGGCAAAGCCCTGACCAGTGGCTCAGTAAGTTCAATATTCTCCGGCCTGGTGGGCGCAGCGCCCGCAGCGGtt                                      <  1:2534386/93‑1 (MQ=255)

                                                   aCAACCAGATCATCAACGGCGGCAAAGCCCTGACCAGTGGCTCAGTAAGTTCAATATTCTCCGGCCTGGTGGGCGCAGCGCCCGCAGCGGttt                                     <  2:791085/93‑1 (MQ=255)

                                                      aCCAGATCATCAACGGCGGCAAAGCCCTGACCAGTGGCTCAGTAAGTTCAATATTCTCCGGCCTGGTGGGCGCAGCGCCCGCAGCGGTTtata                                  <  1:2565607/93‑1 (MQ=255)

                                                       ccAGATCATCAACGGCGGCAAAGCCCTGACCAGTGGCTCAGTAAGTTCAATATTCTCCGGCCTGGTGGGCGCAGCGCCCGCAGCGGTTTatat                                 <  1:3010317/93‑1 (MQ=255)

                                                        cAGATCATCAACGGCGGCAAAGCCCTGACCAGTGGCTCAGTAAGTTCAATATTCTCCGGCCTGGTGGGCGCAGCGCCCGCAGCGGTTTATATc                                <  2:3368823/93‑1 (MQ=255)

                                                         aGATCATCAACGGCGGCAAAGCCCTGACCAGTGGCTCAGTAAGTTCAATATTCTCCGGCCTGGTGGGCGCAGCGCCCGCAGCGGTTTATATCg                               >  1:75232/1‑93 (MQ=255)

                                                          gATCATCAACGGCGGCAAAGCCCTGACCAGTGGCTCAGTAAGTTCAATATTCTCCGGCCTGGTGGGCGCAGCGCCCGCAGCGGTTTATATCGa                              >  1:2366759/1‑93 (MQ=255)

                                                          gATCATCAACGGCGGCAAAGCCCTGACCAGTGGCTCAGTAAGTTCAATATTCTCCGGCCTGGTGGGCGCAGCGCCCGCAGCGGTTTATATCGa                              >  2:505492/1‑93 (MQ=255)

                                                           atcatcAACGGCGGCAAAGCCCTGACCAGTGGCTCAGTAAGTTCAATATTCTCCGGCCTGGTGGGCGCAGCGCCCGCAGCGGTTTATATCGaa                             >  2:4385522/1‑93 (MQ=255)

                                                           atcatcAACGGCGGCAAAGCCCTGACCAGTGGCTCAGTAAGTTCAATATTCTCCGGCCTGGTGGGCGCAGCGCCCGCAGCGGTTTATATCGaa                             >  2:4016757/1‑93 (MQ=255)

                                                            tcatcaACGGCGGCAAAGCCCTGACCAGTGGCTCAGTAAGTTCAATATTCTCCGGCCTGTTGGGCGCGGCGCCCGCAGCGGTTTATATCGAAt                            >  1:2052839/1‑93 (MQ=255)

                                                            tcatcaACGGCGGCAAAGCCCTGACCAGTGGCTCAGTAAGTTCAATATTCTCCGGCCTGGTGGGCGCAGCGCCCGCAGCGGTTTATATCgcac                            >  1:1317094/1‑90 (MQ=255)

                                                            tcatcaACGGCGGCAAAGCCCTGACCAGTGGCTCAGTAAGTTCAATATTCTCCGGCCTGGTGGGCGCAGCGCCCGCAGCGGTTTATATCGAAt                            >  1:2941615/1‑93 (MQ=255)

                                                            tcatcaACGGCGGCAAAGCCCTGACCAGTGGCTCAGTAAGTTCAATATTCTCCGGCCTGGTGGGCGCAGCGCCCGCAGCGGTTTATATCGAAt                            >  1:3968749/1‑93 (MQ=255)

                                                             catcaACGGCGGCAAAGCCCTGACCAGTGGCTCAGTAAGTTCAATATTCTCCGGCCTGGTGGGCGCAGCGCCCGCAGCGGTTTATATCGAATc                           <  1:1397726/93‑1 (MQ=255)

                                                             catcaACGGCGGCAAAGCCCTGACCAGTGGCTCAGTAAGTTCAATATTCTCCGGCCTGGTGGGCGCAGCGCCCGCAGCGGTTTATATCGAATc                           <  1:4636823/93‑1 (MQ=255)

                                                               tcaACGGCGGCAAAGCCCTGACCAGTGGCTCAGTAAGTTCAATATTCTCCGGCCTGGTGGGCGCAGCGCCCGCAGCGGTTTATATCGAATCAg                         <  1:2835093/93‑1 (MQ=255)

                                                                caACGGCGGCAAAGCCCTGACCAGTGGCTCAGTAAGTTCAATATTCTCCGGCCTGGTGGGCGCAGCGCCCGCAGCGGTTTATATCGAATCAGc                        <  1:3221848/93‑1 (MQ=255)

                                                                caACGGCGGCAAAGCCCTGACCAGTGGCTCAGTAAGTTCAATATTCTCCGGCCTGGTGGGCGCAGCGCCCGCAGCGGTTTATATCGAATCAGc                        <  1:2539764/93‑1 (MQ=255)

                                                                caACGGCGGCAAAGCCCTGACCAGTGGCTCAGTAAGTTCAATATTCTCCGGCCTGGTGGGCGCAGCGCCCGCAGCGGTTTATATCGAATCAGc                        <  2:1423544/93‑1 (MQ=255)

                                                                 aaCGGCGGCAAAGCCCTGACCAGTGGCTCAGTAAGTTCAATATTCTCCGGCCTGGTGGGCGCAGCGCCCGCAGCGGTTTATATCGAATCAGCg                       >  1:111334/1‑93 (MQ=255)

                                                                  aCGGCGGCAAAGCCCTGACCAGTGGCTCAGTAAGTTCAATATTCTCCGGCCTGGTGGGCGCAGCGCCCGCAGCGGTTTATATCGAATCAGCgg                      <  2:3838778/93‑1 (MQ=255)

                                                                   cggcggCAAAGCCCTGACCAGTGGCTCAGTAAGTTCAATATTCTCCGGCCTGGTGGGCGCAGCGCCCGCAGCGGTTTATATCGAATCAGCGGc                     >  2:2891481/1‑93 (MQ=255)

                                                                   cggcggCAAAGCCCTGACCAGTGGCTCAGTAAGTTCAATATTCTCCGGCCTGGTGGGCGCAGCGCCCGCAGCGGTTTATATCGAATCAGCGGc                     >  1:3039141/1‑93 (MQ=255)

                                                                    ggcggcAAAGCCCTGACCAGTGGCTCAGTAAGTTCAATATTCTCCGGCCTGGTGGGCGCAGCGCCCGCAGCGGTTTATATCGAATCAGCGGCa                    <  1:951122/93‑1 (MQ=255)

                                                                    ggcggcAAAGCCCTGACCAGTGGCTCAGTAAGTTCAATATTCTCCGGCCTGGTGGGCGCAGCGCCCGCAGCGGTTTATATCGAATCAGCGGCa                    <  1:156127/93‑1 (MQ=255)

                                                                      cggcAAAGCCCTGACCAGTGGCTCAGTAAGTTCAATATTCTCCGGCCTGGTGGGCGCAGCGCCCGCAGCGGTTTATATCGAATCAGCGGCAgg                  <  1:1280819/93‑1 (MQ=255)

                                                                        gcAAAGCCCTGACCAGTGGCTCAGTAAGTTCAATATTCTCCGGCCTGGTGGGCGCAGCGCCCGCAGCGGTTTATATCGAATCAGCGGCAGGaa                >  2:4263790/1‑93 (MQ=255)

                                                                         cAAAGCCCTGACCAGTGGCTCAGTAAGTTCAATATTCTCCGGCCTGGTGGGCGCAGCGCCCGCAGCGGTTTATATCGAATCAGCGGCAGGAAc               <  1:4351915/93‑1 (MQ=255)

                                                                         cAAAGCCCTGACCAGTGGCTCAGTAAGTTCAATATTCTCCGGCCTGGTGGGCGCAGCGCCCGCAGCGGTTTATATCGAATCAGCGGCAGGAAc               <  2:4036142/93‑1 (MQ=255)

                                                                           aaGCCCTGACCAGTGGCTCAGTAAGTTCAATATTCTCCGGCCTGGTGGGCGCAGCGCCCGCAGCGGTTTATATCGAATCAGCGGCAGGAAccg             >  1:1168488/1‑93 (MQ=255)

                                                                            aGCCCTGACCAGTGGCTCAGTAAGTTCAATATTCTCCGGCCTGGTGGGCGCAGCGCCCGCAGCGGTTTATATCGAATCAGCGGCAGGAAccgc            >  2:3563963/1‑93 (MQ=255)

                                                                            aGCCCTGACCAGTGGCTCAGTAAGTTCAATATTCTCCGGCCTGGTGGGCGCAGCGCCCGCAGCGGTTTATATCGAATCAGCGGCAGGAAccgc            <  2:266040/93‑1 (MQ=255)

                                                                            aGCCCTGACCAGTGGCTCAGTAAGTTCAATATTCTCCGGCCTGGTGGGCGCAGCGCCCGCAGCGGTTTATATCGAATCAGCGGCAGGAAacgc            >  2:4140774/1‑93 (MQ=255)

                                                                             gCCCTGACCAGTGGCTCAGTAAGTTCAATATTCTCCGGCCTGGTGGGCGCAGCGCCCGCAGCGGTTTATATCGAATCAGCGGCAGGGAccgcc           >  2:2449286/1‑93 (MQ=255)

                                                                               ccTGACCAGTGGCTCAGTAAGTTCAATATTCTCCGGCCTGGTGGGCGCAGCGCCCGCAGCGGTTTATATCGAATCAGCGGCAGGAAccgccgc         <  1:2270438/93‑1 (MQ=255)

                                                                                cTGACCAGTGGCTCAGTAAGTTCAATATTCTCCGGCCTGGTGGGCGCAGCGCCCGCAGCGGTTTATATCGAATCAGCGGCAGGAAccgccgcc        <  2:3057284/93‑1 (MQ=255)

                                                                                   aCCAGTGGCTCAGTAAGTTCAATATTCTCCGGCCTGGTGGGCGCAGCGCCCGCAGCGGTTTATATCGAATCAGCGGCAGGAACCGCCGCCggg     <  1:212965/93‑1 (MQ=255)

                                                                                    ccAGTGGCTCAGTAAGTTCAATATTCTCCGGCCTGGTGGGCGCAGCGCCCGCAGCGGTTTATATCGAATCAGCGGCAGGAACCGCCGCCgggg    >  1:2300426/1‑93 (MQ=255)

                                                                                    ccAGTGGCTCAGTAAGTTCAATATTCTCCGGCCTGGTGGGCGCAGCGCCCGCAGCGGTTTATATCGAATCAGCGGCAGGAACCGCCGCCgggg    <  1:4505364/93‑1 (MQ=255)

                                                                                     cAGTGGCTCAGTAAGTTCAATATTCTCCGGCCTGGTGGGCGCAGCGCCCGCAGCGGTTTATATCGAATCAGCGGCAGGAACCGCCGCCggggg   <  2:4608016/93‑1 (MQ=255)

                                                                                     cAGTGGCTCAGTAAGTTCAATATTCTCCGGCCTGGTGGGCGCAGCGCCCGCAGCGGTTTATATCGAATCAGCGGCAGGAACCGCCGCCggggg   >  1:1820781/1‑93 (MQ=255)

                                                                                      aGTGGCTCAGTAAGTTCAATATTCTCCGGCCTGGTGGGCGCAGCGCCCGCAGCGGTTTATATCGAATCAGCGGCAGGAACCGCCGCCgggggg  >  1:1212526/1‑92 (MQ=255)

CTACTGGCACCATCCGTGCCGTCGCCGGTCAGGCGAATTTGTTGGATAAAGACAACCAGATCATCAACGGCGGCAAAGCCCTGACCAGTGACTCAGTAAGTTCAATATTCTCCGGCCTGGTGGGCGCAGCGCCCGCAGCGGTTTATATCGAATCAGCGGCAGGAACCGCCGCCGGGGGT  >  NC_000913/3025973‑3026151

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`