BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,047,558	T→C	V4V (GTA→GTG)	gcvH ←	GcvH

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,047,558	0	T	C	99.6%	784.9 / ‑5.2	249	V4V (GTA→GTG)	gcvH	GcvH
Reads supporting (aligned to +/- strand): ref base T (1/0); new base C (132/116); total (133/116)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.32e-01

Only 100 of 253 total aligned reads displayed.

CTGAGCATGTTCGGTAATACCAACGGTGTAAGTGCCGTCGGCTTCTTTACGCAGCCATTCGTGTTCTTTGCTGTATTTCAGTTCTGCTGGTACGTTGCTCATCAATCAATCTCC‑AAAAAAGTAAATCACGCGA‑CGGCTTTGCCGTT‑ACGCACAAAAACAGGTTTTGTCACTTTAACCGGC  >  NC_000913/3047468‑3047647

cTGAGCATGTTCGGTAATACCAACGGTGTAAGTGCCGTCGGCTTCTTTACGCAGCCATTCGTGTTCTTTGCTGTATTTCAGTTCTGCTGGCAc                                                                                            >  2:1491907/1‑93 (MQ=255)

cTGAGCATGTTCGGTAATACCAACGGTGTAAGTGCCGTCGGCTTCTTTACGCAGCCATTCGTGTTCTTTGCTGTATTTCAGTTCTGCTGGCAc                                                                                            >  2:2852698/1‑93 (MQ=255)

 tGAGCATGTTCGGTAATACCAACGGTGTAAGTGCCGTCGGCTTCTTTACGCAGCCATTCGTGTTCTTTGCTGTATTTCAGTTCTGCTGGCACg                                                                                           >  1:878905/1‑93 (MQ=255)

   aGCATGTTCGGTAATACCAACGGTGTAAGTGCCGTCGGCTTCTTTACGCAGGCATTCGTGTTCTTTGCTGTATTTCAGTTCTGCTGGCACGtt                                                                                         <  1:3468535/93‑1 (MQ=255)

   aGCATGTTCGGTAATACCAACGGTGTAAGTGCCGTCGGCTTCTTTACGCAGCCATTCGTGTTCTTTGCTGTATTTCAGTTCTGCTGGCACGtt                                                                                         <  1:2356536/93‑1 (MQ=255)

   aGCATGTTCGGTAATACCAACGGTGTAAGTGCCGTCGGCTTCTTTACGCAGCCATTCGTGGTCTTTGCTGTATTTCAGTTCTGCTGGCACGtt                                                                                         >  2:1129594/1‑93 (MQ=255)

     cATGTTCGGTAATACCAACGGTGTAAGTGCCGTCGGCTTCTTTACGCAGCCATTCGTGTTCTTTGCTGTATTTCAGTTCTGCTGGCACGTTGc                                                                                       <  1:3058305/93‑1 (MQ=255)

     cATGTTCGGTAATACCAACGGTGTAAGTGCCGTCGGCTTCTTTACGCAGCCATTCGTGTTCTTTGCTGTATTTCAGTTCTGCTGGCACGTTGc                                                                                       <  2:2659887/93‑1 (MQ=255)

     cATGTTCGGTAATACCAACGGTGTAAGTGCCGTCGGCTTCTTTACGCAGCCATTCGTGTTCTTTGCTGTATTTCAGTTCTGCTGGCACGTTGc                                                                                       <  2:1223288/93‑1 (MQ=255)

      aTGTTCGGTAATACCAACGGTGTAAGTGCCGTCGGCTTCTTTACGCAGCCATTCGTGTTCTTTGCTGTATTTCAGTTCTGCTGGCACGTTGCt                                                                                      >  2:348030/1‑93 (MQ=255)

      aTGTTCGGTAATACCAACGGTGTAAGTGCCGTCGGCTTCTTTACGCAGCCATTCGTGTTCTTTGCTGTATTTCAGTTCTGCTGGCACGTTGCt                                                                                      <  2:4328205/93‑1 (MQ=255)

      aTGTTCGGTAATACCAACGGTGTAAGTGCCGTCGGCTTCTTTACGCAGCCATTCGTGTTCTTTGCTGTATTTCAGTTCTGCTGGCACGTTGCt                                                                                      <  1:2252201/93‑1 (MQ=255)

       tGTTCGGTAATACCAACGGTGTAAGTGCCGTCGGCTTCTTTACGCAGCCATTCGTGTTCTTTGCTGTATTTCAGTTCTGCTGGCACGTTGCtc                                                                                     >  2:3994262/1‑93 (MQ=255)

        gTTCGGTAATACCAACGGTGTAAGTGCCGTCGGCTTCTTTACGCAGCCATTCGTGTTCTTTGCTGTATTTCAGTTCTGCTGGCACGTTGCtca                                                                                    <  2:4215205/93‑1 (MQ=255)

        gTTCGGTAATACCAACGGTGTAAGTGCCGTCGGCTTCTTTACGCAGCCATTCGTGTTCTTTGCTGTATTTCAGTTCTGCTGGCACGTTGCtca                                                                                    >  2:897019/1‑93 (MQ=255)

         ttCGGTAATACCAACGGTGTAAGTGCCGTCGGCTTCTTTACGCAGCCATTCGTGTTCTTTGCTGTATTTCAGTTCTGCTGGCACGTTGCtcat                                                                                   <  1:2457484/93‑1 (MQ=255)

              tAATACCAACGGTGTAAGTGCCGTCGGCTTCTTTACGCAGCCATTCGTGTTCTTTGCTGTATTTCAGTTCTGCTGGCACGTTGCTCatcaatc                                                                              <  2:2412771/93‑1 (MQ=255)

                aTACCAACGGTGTAAGTGCCGTCGGCTTCTTTACGCAGCCATTCGTGTTCTTTGCTGTATTTCAGTTCTGCTGGCACGTTGCTCAtcaatcaa                                                                            <  1:3198084/93‑1 (MQ=255)

                aTACCAACGGTGTAAGTGCCGTCGGCTTCTTTACGCAGCCATTCGTGTTCTTTGCTGTATTTCAGTTCTGCTGGCACGTTGCTCAtcaatcaa                                                                            >  1:2882931/1‑93 (MQ=255)

                  aCCAACGGTGTAAGTGCCGTCGGCTTCTTTACGCAGCCATTCGTGTTCTTTGCTGTATTTCAGTTCTGCTGGCACGTTGCTCATCaatcaatc                                                                          <  2:1006668/93‑1 (MQ=255)

                  aCCAACGGTGTAAGTGCCGTCGGCTTCTTTACGCAGCCATTCGTGTTCTTTGCTGTATTTCAGTTCTGCTGGCACGTTGCTCATCaatcaatc                                                                          >  1:808640/1‑93 (MQ=255)

                    cAACGGTGTAAGTGCCGTCGGCTTCTTTACGCAGCCATTCGTGTTCTTTGCTGTATTTCAGTTCTGCTGGCACGTTGCTCATCAATCAAtctc                                                                        >  1:208241/1‑93 (MQ=255)

                     aaCGGTGTAAGTGCCGTCGGCTTCTTTACGCAGCCATTCGTGTTCTTTGCTGTATTTCAGTTCTGCTGGCACGTTGCTCATCAATCAATCTcc                                                                       <  2:2140486/93‑1 (MQ=255)

                     aaCGGTGTAAGTGCCGTCGGCTTCTTTACGCAGCCATTCGTGTTCTTTGCTGTATTTCAGTTCTGCTGGCACGTTGCTCATCAATCAATCTcc                                                                       >  1:180930/1‑93 (MQ=255)

                     aaCGGTGTAAGTGCCGTCGGCTTCTTTACGCAGCCATTCGTGTTCTTTGCTGTATTTCAGTTCTGCTGGCACGTTGCTCATCAATCAATCTcc                                                                       >  1:4078820/1‑93 (MQ=255)

                     aaCGGTGTAAGTGCCGTCGGCTTCTTTACGCAGCCATTCGTGTTCTTTGCTGTATTTCAGTTCTGCTGGCACGTTGCTCATCAATCAATCTcc                                                                       <  1:1476406/93‑1 (MQ=255)

                     aaCGGTGTAAGTGCCGTCGGCTTCTTTACGCAGCCATTCGTGTTCTTTGCTGTATTTCAGTTCTGCTGGCACGTTGCTCATCAATCAATCTcc                                                                       >  1:1486842/1‑93 (MQ=255)

                       cGGTGTAAGTGCCGTCGGCTTCTTTACGCAGCCATTCGTGTTCTTTGCTGTATTTCAGTTCTGCTGGCACGTTGCTCATCAATCAATCTCC‑aa                                                                    <  2:3367044/93‑1 (MQ=255)

                        ggTGTAAGTGCCGTCGGCTTCTTTACGCAGCCATTCGTGTTCTTTGCTGTATTTCAGTTCTGCTGGCACGTTGCTCATCAATCAATCTCC‑aaa                                                                   >  1:3178997/1‑93 (MQ=255)

                        ggTGTAAGTGCCGTCGGCTTCTTTACGCAGCCATTCGTGTTCTTTGCTGTATTTCAGTTCTGCTGGCACGTTGCTCATCAATCAATCTCC‑aaa                                                                   >  2:4475351/1‑93 (MQ=255)

                        ggTGTAAGTGCCGTCGGCTTCTTTACGCAGCCATTCGTGTTCTTTGCTGTATTTCAGTTCTGCTGGCACGTTGCTCATCAATCAATCTAC‑aca                                                                   >  1:1174415/1‑91 (MQ=255)

                         gtgtAAGTGCCGTCGGCTTCTTTACGCAGCCATTCGTGTTCTTTGCTGTATTTCAGTTCTGCTGGCACGTTGCTCATCAATCAATCTCC‑aaaa                                                                  >  1:2824318/1‑93 (MQ=255)

                           gtAAGTGCCGTCGGCTTCTTTACGCAGCCATTCGTGTTCTTTGCTGTATTTCAGTTCTGCTGGCACGTTGCTCATCAATCAATCTCC‑aaaaaa                                                                <  2:3094390/93‑1 (MQ=255)

                           gtAAGTGCCGTCGGCTTCTTTACGCAGCCATTCGTGTTCTTTGCTGTATTTCAGTTCTGCTGGCACGTTGCTCATCAATCAATCTCC‑aaaaaa                                                                >  1:2176287/1‑93 (MQ=255)

                            tAAGTGCCGTCGGCTTCTTTACGCAGCCATTCGTGTTCTTTGCTGTATTTCAGTTCTGCTGGCACGTTGCTCATCAATCAATCTCC‑AAAAAAg                                                               >  2:3299399/1‑93 (MQ=255)

                            tAAGTGCCGTCGGCTTCTTTACGCAGCCATTCGTGTTCTTTGCTGTATTTCAGTTCTGCTGGCACGTTGCTCATCAATCAATCTCC‑AAAAAAg                                                               >  2:3394783/1‑93 (MQ=255)

                              aGTGCCGTCGGCTTCTTTACGCAGCCATTCGTGTTCTTTGCTGTATTTCAGTTCTGCTGGCACGTTGCTCATCAATCAATCTCC‑AAAAAAGTa                                                             <  2:2506339/93‑1 (MQ=255)

                              aGTGCCGTCGGCTTCTTTACGCAGCCATTCGTGTTCTTTGCTGTATTTCAGTTCTGCTGGCACGTTGCTCATCAATCAATCTCC‑AAAAAAGTa                                                             <  2:1311512/93‑1 (MQ=255)

                               gTGCCGTCGGCTTCTTTACGCAGCCATTCGTGTTCTTTGCTGTATTTCAGTTCTGCTGGCACGTTGCTCATCAATCAATCTCC‑AAAAAAGTaa                                                            <  1:3099024/93‑1 (MQ=255)

                               gTGCCGTCGGCTTCTTTACGCAGCCATTCGTGTTCTTTGCTGTATTTCAGTTCTGCTGGCACGTTGCTCATCAATCAATCTCC‑AAAAAAGTaa                                                            <  1:4285201/93‑1 (MQ=255)

                               gTGCCGTCGGCTTCTTTACGCAGCCATTCGTGTTCTTTGCTGTATTTCAGTTCTGCTGGCACGTTGCTCATCAATCAATCTCC‑AAAAAAGTaa                                                            >  1:1278103/1‑93 (MQ=255)

                               gTGCCGTCGGCTTCTTTACGCAGCCATTCGTGTTCTTTGCTGTATTTCAGTTCTGCTGGCACGTTGCTCATCAATCAATCTCC‑AAAAAAGTaa                                                            >  1:2849903/1‑93 (MQ=255)

                                ttccGTCGGCTTCTTTACGCAGCCATTCGTGTTCTTTGCTGTATTTCAGTTCTGCTGGCACGTTGCTCATCAATCAATCTCC‑AAAAAAGTaaa                                                           <  2:1757360/91‑1 (MQ=255)

                                tGCCGTCGGCTTCTTTACGCAGCCATTCGTGTTCTTTGCTGTATTTCAGTTCTGCTGGCACGTTGCTCATCAATCAATCTCC‑AAAAAAGTaaa                                                           <  1:3991907/93‑1 (MQ=255)

                                 gCCGTCGGCTTCTTTACGCAGCCATTCGTGTTCTTTGCTGTATTTCAGTTCTGCTGGCACGTTGCTCATCAATCAATCTCC‑AAAAAAGTAAAt                                                          <  2:1665458/93‑1 (MQ=255)

                                 gCCGTCGGCTTCTTTACGCAGCCATTCGTGTTCTTTGCTGTATTTCAGTTCTGCTGGCACGTTGCTCATCAATCAATCTCC‑AAAAAAGTAAAt                                                          >  2:830168/1‑93 (MQ=255)

                                  ccGTCGGCTTCTTTACGCAGCCATTCGTGTTCTTTGCTGTATTTCAGTTCTGCTGGCACGTTGCTCATCAATCAATCTCC‑AAAAAAGTAAATc                                                         <  1:2664566/93‑1 (MQ=255)

                                   cGTCGGCTTCTTTACGCAGCCATTCGTGTTCTTTGCTGTATTTCAGTTCTGCTGGCACGTTGCTCATCAATCAATCTCC‑AAAAAAGTAAATCa                                                        <  2:2003834/93‑1 (MQ=255)

                                    gTCGGCTTCTTTACGCAGCCATTCGTGTTCTTTGCTGTATTTCAGTTCTGCTGGCACGTTGCTCATCAATCAATCTCC‑AAAAAAGTAAATCAc                                                       <  2:453117/93‑1 (MQ=255)

                                     tCGGCTTCTTTACGCAGCCATTCGTGTTCTTTGCTGTATTTCAGTTCTGCTGGCACGTTGCTCATCAATCAATCTCC‑AAAAAAGTAAATCAcg                                                      >  1:3679366/1‑93 (MQ=255)

                                     tCGGCTTCTTTACGCAGCCATTCGTGTTCTTTGCTGTATTTCAGTTCTGCTGGCACGTTGCTCATCAATCAATCTCC‑AAAAAAGTAAATCAcg                                                      <  2:1442297/93‑1 (MQ=255)

                                        gCTTCTTTACGCAGCCATTCGTGTTCTTTGCTGTATTTCAGTTCTGCTGGCACGTTGCTCATCAATCAATCTCC‑AAAAAAGTAAATCACGCGa                                                   >  2:4085112/1‑93 (MQ=255)

                                          ttcttTACGCAGCCATTCGTGTTCTTTGCTGTATTTCAGTTCTGCTGGCACGTTGCTCATCAATCAATCTCC‑AAAAAAGTAAATCACGCGA‑Cg                                                <  1:4594553/93‑1 (MQ=255)

                                            cttTACGCAGCCATTCGTGTTCTTTGCTGTATTTCAGTTCTGCTGGCACGTTGCTCATCAATCAATCTCC‑AAAAAAGTAAATCACGCGA‑CGGc                                              <  1:270840/93‑1 (MQ=255)

                                             tttACGCAGCCATTCGTGTTCTTTGCTGTATTTCAGTTCTGCTGGCACGTTGCTCATCAATCAATCTCC‑AAAAAAGTAAATCACGCGA‑CGGCt                                             >  1:4610757/1‑93 (MQ=255)

                                             tttACGCAGCCATTCGTGTTCTTTGCTGTATTTCAGTTCTGCTGGCACGTTGCTCATCAATCAATCTCC‑AAAAAAGTAAATCACGCGA‑CGGCt                                             >  2:1824979/1‑93 (MQ=255)

                                             tttACGCAGCCATTCGTGTTCTTTGCTGTATTTCAGTTCTGCTGGCACGTTGCTCATCAATCAATCTCC‑AAAAAAGTAAATCACGCGA‑CGGCt                                             <  1:3644842/93‑1 (MQ=255)

                                              ttACGCAGCCATTCGTGTTCTTTGCTGTATTTCAGTTCTGCTGGCACGTTGCTCATCAATCAATCTCC‑AAAAAAGTAAATCACGCGA‑CGGCtt                                            >  2:2267997/1‑93 (MQ=255)

                                                aCGCAGCCATTCGTGTTCTTTGCTGTATTTCAGTTCTGCTGGCACGTTGCTCATCAATCAATCTCC‑AAAAAAGTAAATCACGCGA‑CGGCTTTg                                          >  2:721600/1‑93 (MQ=255)

                                                 cGCAGCCATTCGTGTTCTTTGCTGTATTTCAGTTCTGCTGGCACGTTGCTCATCAATCAATCTCC‑AAAAAAGTAAATCACGCGA‑CGGCTTTGc                                         <  2:2049356/93‑1 (MQ=255)

                                                    aGCCATTCGTGTTCTTTGCTGTATTTCAGTTCTGCTGGCACGTTGCTCATCAATCAATCTCC‑AAAAAAGTAAATCACGCGA‑CGGCTTTGCCGt                                      <  2:930557/93‑1 (MQ=255)

                                                      ccATTCGTGTTCTTTGCTGTATTTCAGTTCTGCTGGCACGTTGCTCATCAATCAATCTCC‑AAAAAAGTAAATCACGCGA‑CGGCTTTGCCGTT‑a                                   <  1:1009324/93‑1 (MQ=255)

                                                      ccATTCGTGTTCTTTGCTGTATTTCAGTTCTGCTGGCACGTTGCTCATCAATCAATCTCC‑AAAAAAGTAAATCACGCGA‑CGGCTTTGCCGTT‑a                                   <  2:3850443/93‑1 (MQ=255)

                                                      ccATTCGTGTTCTTTGCTGTATTTCAGTTCTGCTGGCACGTTGCTCATCAATCAATCTCC‑AAAAAAGTAAATCACGCGA‑CGGCTTTGCCGTT‑a                                   <  1:3885535/93‑1 (MQ=255)

                                                      ccATTCGTGTTCTTTGCTGTATTTCAGTTCTGCTGGCACGTTGCTCATCAATCAATCTCC‑AAAAAAGTAAATCACGCGA‑CGGCTTTGCCGTT‑a                                   >  2:2187611/1‑93 (MQ=255)

                                                       cATTCGTGTTCTTTGCTGTATTTCAGTTCTGCTGGCACGTTGCTCATCAATCAATCTCC‑AAAAAAGTAAATCACGCGA‑CGGCTTTGCCGTT‑Ac                                  <  1:1351870/93‑1 (MQ=255)

                                                        aTTCGTGTTCTTTGCTGTATTTCAGTTCTGCTGGCACGTTGCTCATCAATCAATCTCC‑AAAAAAGTAAATCACGCGA‑CGGCTTTGTCGTT‑ACg                                 >  1:4212504/1‑93 (MQ=255)

                                                         ttCGTGTTCTTTGCTGTATTTCAGTTCTGCTGGCACGTTGCTCATCAATCAATCTCCAAAAAAAGTAAATCACGCGA‑CGGCTTTGCCGTT‑ACg                                 >  2:495698/1‑93 (MQ=255)

                                                         ttCGTGTTCTTTGCTGTATTTCAGTTCTGCTGGCACGTTGCTCATCAATCAATCTCC‑AAAAAAGTAAATCACGCGA‑CGGCTTTGCCGTT‑ACGc                                >  2:2936852/1‑93 (MQ=255)

                                                          tCGTGTTCTTTGCTGTATTTCAGTTCTGCTGGCACGTTGCTCATCAATCAATCTCC‑AAAAAAGTAAATCACGCGA‑CGGCTTTGCCGTT‑ACGca                               <  2:784452/93‑1 (MQ=255)

                                                            gtgtTCTTTGCTGTATTTCAGTTCTGCTGGCACGTTGCTCATCAATCAATCTCC‑AAAAAAGTAAATCACGCGA‑CGGCTTTGCCGTT‑ACGcaca                             >  2:2094463/1‑93 (MQ=255)

                                                            gtgtTCTTTGCTGTATTTCAGTTCTGCTGGCACGTTGCTCATCAATCAATCTCC‑AAAAAAGTAAATCACGCGA‑CGGCTTTGCCGTT‑ACGcaca                             >  1:1793314/1‑93 (MQ=255)

                                                            gtgtTCTTTGCTGTATTTCAGTTCTGCTGGCACGTTGCTCATCAATCAATCTCC‑AAAAAAGTAAATCACGCGA‑CGGCTTTGCCGTT‑ACGcaca                             >  1:1856965/1‑93 (MQ=255)

                                                             tgtTCTTTGCTGTATTTCAGTTCTGCTGGCACGTTGCTCATCAATCAATCTCC‑AAAAAAGTAAATCACGCGA‑CGGCTTTGCCGTT‑ACGCACaa                            >  2:4106524/1‑93 (MQ=255)

                                                                tCTTTGCTGTATTTCAGTTCTGCTGGTACGTTGCTCATCAATCAATCTCC‑AAAAAAGTAAATCACGCGA‑CGGCTTTGCCGTT‑ACGCACaaaaa                         >  1:2937674/1‑93 (MQ=255)

                                                                 cTTTGCTGTATTTCAGTTCTGCTGGCACGTTGCTCATCAATCAATCTCC‑AAAAAAGTAAATCACGCGA‑CGGCTTTGCCGTT‑ACGCACAAAAAc                        <  2:3790288/93‑1 (MQ=255)

                                                                  tttGCTGTATTTCAGTTCTGCTGGCACGTTGCTCATCAATCAATCTCC‑AAAAAAGTAAATCACGCGA‑CGGCTTTGCCGTT‑ACGCACAAAAACa                       >  1:1214715/1‑93 (MQ=255)

                                                                   ttGCTGTATTTCAGTTCTGCTGGCACGTTGCTCATCAATCAATCTCC‑AAAAAAGTAAATCACGCGA‑CGGCTTTGCCGTT‑ACGCACAAAAACAg                      <  1:276681/93‑1 (MQ=255)

                                                                    tGCTGTATTTCAGTTCTGCTGGCACGTTGCTCATCAATCAATCTCC‑AAAAAAGTAAATCACGCGA‑CGGCTTTGCCGTT‑ACGCACAAAAACAgg                     <  1:4445206/93‑1 (MQ=255)

                                                                     gCTGTATTTCAGTTCTGCTGGCACGTTGCTCATCAATCAATCTCC‑AAAAAAGTAAATCACGCGA‑CGGCTTTGCCGTT‑ACGCACAAAAACAGGt                    <  2:3609345/93‑1 (MQ=255)

                                                                      cTGTATTTCAGTTCTGCTGGCACGTTGCTCATCAATCAATCTCC‑AAAAAAGTAAATCACGCGA‑CGGCTTTGCCGTT‑ACGCACAAAAACAGGtt                   <  1:3582053/93‑1 (MQ=255)

                                                                       tGTATTTCAGTTCTGCTGGCACGTTGCTCATCAATCAATCTCC‑AAAAAAGTAAATCACGCGA‑CGGCTTTGCCGTT‑ACGCACAAAAACAGGttt                  >  1:3787162/1‑93 (MQ=255)

                                                                       tGTATTTCAGTTCTGCTGGCACGTTGCTCATCAATCAATCTCC‑AAAAAAGTAAATCACGCGA‑CGGCTTTGCCGTT‑ACGCACAAAAACAGGttt                  >  1:1154304/1‑93 (MQ=255)

                                                                         tATTTCAGTTCTGCTGGCACGTTGCTCATCAATCAATCTCC‑AAAAAAGTAAATCACGCGA‑CGGCTTTGCCGTT‑ACGCACAAAAACAGGTTTTg                >  2:1403464/1‑93 (MQ=255)

                                                                           tttCAGTTCTGCTGGCACGTTGCTCATCAATCAATCTCC‑AAAAAAGTAAATCACGCGA‑CGGCTTTGCCGTT‑ACGCACAAAAACAGGTTTTGTc              >  1:4669648/1‑93 (MQ=255)

                                                                           tttCAGTTCTGCTGGCACGTTGCTCATCAATCAATCTCC‑AAAAAAGTAAATCACGCGA‑CGGCTTTGCCGTT‑ACGCACAAAAACAGGTTTTGTc              <  2:3635982/93‑1 (MQ=255)

                                                                           tttCAGTTCTGCTGGCACGTTGCTCATCAATCAATCTCC‑AAAAAAGTAAATCACGCGA‑CGGCTTTGCCGTT‑ACGCACAAAAACAGGTTTTGTc              <  1:280547/93‑1 (MQ=255)

                                                                           tttCAGTTCTGCTGGCACGTTGCTCATCAATCAATCTCC‑AAAAAAGTAAAACACGCGACCGGCTTTTCCGTTCACCCAAAAAAAAAAGttttt                >  2:2821543/1‑92 (MQ=255)

                                                                             tCAGTTCTGCTGGCACGTTGCTCATCAATCAATCTCC‑AAAAAAGTAAATCACGCGA‑CGGCTTTGCCGTT‑ACGCACAAAAACAGGTTTTGTCAc            >  1:2549142/1‑93 (MQ=255)

                                                                             tCAGTTCTGCTGGCACGTTGCTCATCAATCAATCTCC‑AAAAAAGTAAATCACGCGA‑CGGCTTTGCCGTT‑ACGCACAAAAACAGGTTTTGTCAc            <  1:1913093/93‑1 (MQ=255)

                                                                               aGTTCTGCTTGCACGTTGCTCATCAATCAATCTCC‑AAAAAAGTAAATCACGCGA‑CGGCTTTGCCGTT‑ACGCACAAAAACAGGTTTTGTCACtt          <  1:3932236/93‑1 (MQ=255)

                                                                               aGTTCTGCTGGCACGTTGCTCATCAATCAATCTCC‑AAAAAAGTAAATCACGCGA‑CGGCTTTGCCGTT‑ACGCACAAAAACAGGTTTTGTCACtt          <  2:662919/93‑1 (MQ=255)

                                                                               aGTTCTGCTGGCACGTTGCTCATCAATCAATCTCC‑AAAAAAGTAAATCACGCGA‑CGGCTTTGCCGTT‑ACGCACAAAAACAGGTTTTGTCACtt          >  1:2335035/1‑93 (MQ=255)

                                                                               aGTTCTGCTGGCACGTTGCTCATCAATCAATCTCC‑AAAAAAGTAAATCACGCGA‑CGGCTTTGCCGTT‑ACGCACAAAAACAGGTTTTGTCACtt          <  1:1301016/93‑1 (MQ=255)

                                                                               aGTTCTGCTGGCACGTTGCTCATCAATCAATCTCC‑AAAAAAGTAAATCACGCGA‑CGGCTTTGCCGTT‑ACGCACAAAAACAGGTTTTGTCACtt          <  1:1131067/93‑1 (MQ=255)

                                                                                attCTGCTGGCACGTTGCTCATCAATCAATCTCC‑AAAAAAGTAAATCACGCGA‑CGGCTTTGCCGTT‑ACGCACAAAAACAGGTTTTGTCACttt         <  2:2339743/92‑1 (MQ=255)

                                                                                 ttCTGCTGGCACGTTGCTCATCAATCAATCTCC‑AAAAAAGTAAATCACGCGA‑CGGCTTTGCCGTT‑ACGCACAAAAACAGGTTTTGTCACTTTa        >  1:4375067/1‑93 (MQ=255)

                                                                                  tCTGCTGGCACGTTGCTCATCAATCAATCTCC‑AAAAAAGTAAATCACGCGA‑CGGCTTTGCCGTT‑ACGCACAAAAACAGGTTTTGTCACTTTaa       <  1:4519824/93‑1 (MQ=255)

                                                                                     gctgGCACGTTGCTCATCAATCAATCTCC‑AAAAAAGTAAATCACGCGA‑CGGCTTTGCCGTT‑ACGCACAAAAACAGGTTTTGTCACTTTAACCg    >  2:2626720/1‑93 (MQ=255)

                                                                                       tgGCACGTTGCTCATCAATCAATCTCC‑AAAAAAGTAAATCACGCGA‑CGGCTTTGCCGTT‑ACGCACAAAAACAGGTTTTGTCACTTTAACCGGc  <  1:3724599/93‑1 (MQ=255)

CTGAGCATGTTCGGTAATACCAACGGTGTAAGTGCCGTCGGCTTCTTTACGCAGCCATTCGTGTTCTTTGCTGTATTTCAGTTCTGCTGGTACGTTGCTCATCAATCAATCTCC‑AAAAAAGTAAATCACGCGA‑CGGCTTTGCCGTT‑ACGCACAAAAACAGGTTTTGTCACTTTAACCGGC  >  NC_000913/3047468‑3047647

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 31 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`