BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,078,221	C→T	Q478Q (CAG→CAA)	tktA ←	transketolase 1 isozyme

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,078,221	0	C	T	100.0%	683.2 / NA	190	Q478Q (CAG→CAA)	tktA	transketolase 1 isozyme
Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (93/97); total (93/97)

Only 100 of 195 total aligned reads displayed.

CTCAACACCGTATTTCCACGCGACCGCGGATTCAACCTGGTCACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGCCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCATCAGCGC  >  NC_000913/3078131‑3078310

cTCAACGCCGTATTTCCACGCGACCGCGGATTCAACCTGGTCACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACTTg                                                                                         >  1:3524439/1‑93 (MQ=255)

 tCAACACCGTATTTCCACGCGACCGCGGATTCAACCTGGTCACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGCAGCGACTTGc                                                                                        >  2:396167/1‑93 (MQ=255)

 tCAACACCGTATTTCCACGCGACCGCGGATTCAACCTGGTCACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACTTGc                                                                                        >  1:4423177/1‑93 (MQ=255)

   aaCACCGTATTTCCACGCGACCGCGGATTCAACCTGGTCACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACTTGCTc                                                                                      >  1:1738623/1‑93 (MQ=255)

    acacCGTATTTCCACGCGACCGCGGATTCAACCTGGTCACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACTTGCTCa                                                                                     >  1:209362/1‑93 (MQ=255)

    acacCGTATTTCCACGCGACCGCGGATTCAACCTGGTCACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACTTGCTCa                                                                                     <  1:2696918/93‑1 (MQ=255)

       ccGTATTTCCACGCGACCGCGGATTCAACCTGGTCACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACTTGCTCAAcc                                                                                  >  1:2407575/1‑93 (MQ=255)

         gTATTTCCACGCGACCGCGGATTCAACCTGGTCACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACTTGCTCAACCgg                                                                                >  1:231753/1‑93 (MQ=255)

          tATTTCCACGCGACCGCGGATTCAACCTGGTCACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACTTGCTCAACCGGc                                                                               >  2:807222/1‑93 (MQ=255)

            tttCCACGCGACCGCGGATTCAACCTGGTCACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACTTGCTCAACCGGCTg                                                                             >  1:3169239/1‑93 (MQ=255)

            ttcccACGCGACCGCGGATTCAACGTGGTCACACAGACGCCATGTAGACATGTTCGGGGTTACGCGCAAAGAAGCGACTTGCTCAACCGGCTg                                                                             <  2:2504591/90‑1 (MQ=255)

             ttCCACGCGACCGCGGATTCAACCTGGTCACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACTTGCTCAACCGGCTgg                                                                            <  2:3931100/93‑1 (MQ=255)

             ttCCACGCGACCGCGGATTCAACCTGGTCACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACTTGCTCAACCGGCTgg                                                                            <  2:271748/93‑1 (MQ=255)

              tCCACGCGACCGCGGATTCAACCTGGTCACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACTTGCTCAACCGGCTGGt                                                                           <  2:1017179/93‑1 (MQ=255)

               ccACGCGACCGCGGATTCAACCTGGTCACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACTTGCTCAACCGGCTGGTg                                                                          >  1:3489793/1‑93 (MQ=255)

                 aCGCGACCGCGGATTCAACCTGGTCACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACTTGCTCAACCGGCTGGTGAg                                                                        >  2:551903/1‑93 (MQ=255)

                    cgACCGCGGATTCAACCTGGTCACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACTTGCTCAACCGGCTGGTGAGTCg                                                                     >  2:760200/1‑93 (MQ=255)

                     gACCGCGGATTCAACCTGGTCACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACTTGCTCAACCGGCTGGTGAGTCgg                                                                    >  1:2048460/1‑93 (MQ=255)

                     gACCGCGGATTCAACCTGGTCACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACTTGCTCAACCGGCTGGTGAGTCgg                                                                    <  2:2723296/93‑1 (MQ=255)

                      aCCGCGGATTCAACCTGGTCACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACTTGCTCAACCGGCTGGTGAGTCggg                                                                   <  1:1306683/93‑2 (MQ=255)

                      aCCGCGGATTCAACCTGGTCACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACTTGCTCAACCGGCTGGTGAGTCggg                                                                   >  2:4413025/1‑92 (MQ=255)

                       ccGCGGATTCAACCTGGTCACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACTTGCTCAACCGGCTGGTGAGTCgggc                                                                  <  2:1808176/93‑3 (MQ=255)

                       ccGCGGATTCAACCTGGTCACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACTTGCTCAACCGGCTGGTGAGTCgggc                                                                  <  1:3290281/93‑3 (MQ=255)

                         gcgGATTCAACCTGGTCACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACTTGCTCAACCGGCTGGTGAGTCGGGCCg                                                                >  2:1435597/1‑93 (MQ=255)

                            gATTCAACCTGGTCACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACTTGCTCAACCGGCTGGTGAGTCGGGCCGTCt                                                             >  1:3926155/1‑93 (MQ=255)

                            gATTCAACCTGGTCACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACTTGCTCAACCGGCTGGTGAGTCGGGCCGTCt                                                             >  1:980402/1‑93 (MQ=255)

                            gATTCAACCTGGTCACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACTTGCTCAACCGGCTGGTGAGTCGGGCCGTCt                                                             <  2:3386666/93‑1 (MQ=255)

                             aTTCAACCTGGTCACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACTTGCTCAACCGGCTGGTGAGTCGGGCCGTCtt                                                            <  1:2192547/93‑1 (MQ=255)

                              ttCAACCTGGTCACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACTTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTc                                                           >  2:1843052/1‑93 (MQ=255)

                              ttCAACCTGGTCACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACTTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTc                                                           >  1:95041/1‑93 (MQ=255)

                              ttCAACCTGGTCACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACTTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTc                                                           >  1:1058619/1‑93 (MQ=255)

                               tCAACCTGGTCACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACTTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCg                                                          >  1:1088451/1‑93 (MQ=255)

                                 aaCCTGGTCACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACTTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGcc                                                        >  1:1918935/1‑93 (MQ=255)

                                 aaCCTGGTCACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACTTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGcc                                                        <  2:4359092/93‑1 (MQ=255)

                                 aaCCTGGTCACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACTTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGcc                                                        >  2:3160715/1‑93 (MQ=255)

                                  aCCTGGTCACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACTTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGccc                                                       >  1:3523991/1‑93 (MQ=255)

                                   ccTGGTCACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACTTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCa                                                      >  2:2397640/1‑93 (MQ=255)

                                   ccTGGTCACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACTTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCa                                                      <  1:1586300/93‑1 (MQ=255)

                                   ccTGGTCACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACTTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCa                                                      <  1:798517/93‑1 (MQ=255)

                                    cTGGTCACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACTTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAg                                                     >  2:2821564/1‑93 (MQ=255)

                                     tGGTCACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACTTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGa                                                    >  1:3836053/1‑93 (MQ=255)

                                     tGGTCACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACTTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGa                                                    <  1:4656695/93‑1 (MQ=255)

                                      ggTCACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACTTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGAc                                                   <  2:1481004/93‑1 (MQ=255)

                                      ggTCACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACTTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGAc                                                   <  2:1164795/93‑1 (MQ=255)

                                      ggTCACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACTTGCTCAACCGACTGGTGAGTCGGGCCGTCTTCGCCCAGAc                                                   <  2:3791702/93‑1 (MQ=255)

                                        tCACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACTTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCg                                                 >  2:305684/1‑93 (MQ=255)

                                         cacaCGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACTTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGa                                                <  1:2652065/93‑1 (MQ=255)

                                         cacaCGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACTTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGa                                                <  2:3816571/93‑1 (MQ=255)

                                          acacGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACTTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGAt                                               <  2:688666/93‑1 (MQ=255)

                                           cacGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACTTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATg                                              >  1:1806305/1‑93 (MQ=255)

                                            acGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACTTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGCTgg                                             >  1:3742475/1‑93 (MQ=255)

                                             cGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACTTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGa                                            <  2:644106/93‑1 (MQ=255)

                                              ggACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACTTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAg                                           >  2:4445752/1‑93 (MQ=255)

                                               gACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACTTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGt                                          <  2:1325444/93‑1 (MQ=255)

                                               gACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACTTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGt                                          <  1:1532672/93‑1 (MQ=255)

                                                aCGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACTTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAggc                                         >  1:2970338/1‑91 (MQ=255)

                                                aCGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACTTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTc                                         >  1:3091237/1‑93 (MQ=255)

                                                 cGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACTTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCg                                        >  1:2609517/1‑93 (MQ=255)

                                                  gCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACTTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCgg                                       >  2:936622/1‑92 (MQ=255)

                                                  gCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACTTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGt                                       <  2:2951178/93‑1 (MQ=255)

                                                  gCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACTTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGt                                       >  1:122102/1‑93 (MQ=255)

                                                   ccATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACTTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTg                                      <  1:3773048/93‑1 (MQ=255)

                                                   ccATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACTTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTg                                      >  1:882581/1‑93 (MQ=255)

                                                      tGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACTTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGgt                                   >  2:4061717/1‑93 (MQ=255)

                                                         aGACATGTTCGGGGTTACGCGCAGAGAAGCGACTTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTa                                >  2:2638011/1‑93 (MQ=255)

                                                         aGACATGTTCGGGGTTACGCGCAGAGAAGCGACTTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTa                                <  1:4254285/93‑1 (MQ=255)

                                                           aCATGTTCGGGGTTACGCGCAGAGAAGCGACTTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTaaa                              <  1:663713/93‑1 (MQ=255)

                                                           aCATGTTCGGGGTTACGCGCAGAGAAGCGACTTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTaaa                              <  1:1729795/93‑1 (MQ=255)

                                                            cATGTTCGGGGTTACGCGCAGAGAAGCGACTTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAAc                             <  2:4335465/93‑1 (MQ=255)

                                                            cATGTTCGGGGTTACGCGCAGAGAAGCGACTTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAAc                             >  2:4124217/1‑93 (MQ=255)

                                                            cATGTTCGGGGTTACGCGCAGAGAAGCGACTTGCTCAACCGGCTGGTGAGTCGGGACGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAAc                             >  1:4158178/1‑93 (MQ=255)

                                                             aTGTTCGGGGTTACGCGCAGAGAAGCGACTTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAAcc                            >  2:857279/1‑93 (MQ=255)

                                                               gTTCGGGGTTACGCGCAGAGAAGCGACTTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCAt                          <  2:1741235/93‑1 (MQ=255)

                                                               gTTCGGGGTTACGCGCAGAGAAGCGACTTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCAt                          >  2:497378/1‑93 (MQ=255)

                                                                ttCGGGGTTACGCGCAGAGAAGCGACTTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATc                         <  2:4159457/93‑1 (MQ=255)

                                                                ttCGGGGTTACGCGCAGAGAAGCGACTTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATc                         <  2:2323174/93‑1 (MQ=255)

                                                                   ggggTTACGCGCAGAGAAGCGACTTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCAcc                      <  2:2140114/93‑1 (MQ=255)

                                                                   ggggTTACGCGCAGAGAAGCGACTTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCAcc                      <  2:3211020/93‑1 (MQ=255)

                                                                    gggTTACGCGCAGAGAAGCGACTTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCt                     >  2:3613604/1‑93 (MQ=255)

                                                                    gggTTACGCGCAGAGAAGCGACTTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCt                     >  1:4617560/1‑93 (MQ=255)

                                                                      gTTACGCGCAGAGAAGCGACTTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGa                   <  2:2365290/93‑1 (MQ=255)

                                                                      gTTACGCGCAGAGAAGCGACTTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATAACCTGa                   >  2:3259929/1‑93 (MQ=255)

                                                                         aCGCGCAGAGAAGCGACTTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGc                <  2:1369014/93‑1 (MQ=255)

                                                                         aCGCGCAGAGAAGCGACTTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGc                >  1:1471095/1‑93 (MQ=255)

                                                                         aCGCGCAGAGAAGCGACTTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGc                <  2:2309782/93‑1 (MQ=255)

                                                                         aCGCGCAGAGAAGCGACTTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGc                <  2:2545491/93‑1 (MQ=255)

                                                                          cgcgCAGAGAAGCGACTTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCt               <  1:4047556/93‑1 (MQ=255)

                                                                             gcAGAGAAGCGACTTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTgat            >  1:585826/1‑91 (MQ=255)

                                                                             gcAGAGAAGCGACTTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGtt            <  1:2816731/93‑1 (MQ=255)

                                                                             gcAGAGAAGCGACTTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGtt            <  2:1957718/93‑1 (MQ=255)

                                                                              cAGAGAAGCGACTTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGttt           <  1:4479015/93‑1 (MQ=255)

                                                                               agagAAGCGACTTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTtc          >  2:1590096/1‑93 (MQ=255)

                                                                                gagaAGCGACTTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTtca         <  2:3127714/93‑1 (MQ=255)

                                                                                gagaAGCGACTTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGGCGTGGGTGTAAACCATCACCTGACGCTGTTtca         >  2:4552143/1‑93 (MQ=255)

                                                                                 agaAGCGACTTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTtcat        <  1:1861391/93‑1 (MQ=255)

                                                                                  gaAGCGACTTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCACACAGATGGAGTCGTGCGGGTAAACCATCACCCGCCGCTGTTtcctc       >  2:1645801/1‑93 (MQ=255)

                                                                                   aaGCGACTTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTtcatca      <  2:4648326/93‑1 (MQ=255)

                                                                                   aaGCGACTTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTtcatca      >  2:1979237/1‑93 (MQ=255)

                                                                                     gCGACTTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCAGCTGACGCTGTTTCATCAgc    >  1:4306858/1‑93 (MQ=255)

                                                                                       gACTTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCATCAgcgc  >  2:4207295/1‑93 (MQ=255)

CTCAACACCGTATTTCCACGCGACCGCGGATTCAACCTGGTCACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGCCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCATCAGCGC  >  NC_000913/3078131‑3078310

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 17 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`