BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,100,603	A→G	F90L (TTC→CTC)	yggH ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,100,603	0	A	G	99.4%	501.2 / NA	162	F90L (TTC→CTC)	yggH	hypothetical protein
Reads supporting (aligned to +/- strand): ref base A (0/0); major base G (86/75); minor base C (1/0); total (87/75)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 169 total aligned reads displayed.

GACACATCACGCGCAGGTTGCTTAAACCTTCTTCATGCGCAGAAGCCAGGCACGCACCAACGCCCGGTGAATGCACTTCAATGCCGAGGAAGTCCTGCTCAGGGCGATCTTTAGCCATTGCCACCAGCGACGCCCCCATGCCAAAACCAATCTCAAGCGTCACCGGCGCTTCACGGCCAA  >  NC_000913/3100513‑3100692

gACACATCACGCGCAGGTTGCTTAAACCTTCTTCATGCGCAGAAGCCAGGCACGCACCAACGCCCGGTGAATGCACTTCAATGCCGAGGAGGt                                                                                         <  2:2032790/93‑1 (MQ=255)

 acacATCACGCGCAGGTTGCTTAAACCTTCTTCATGCGCAGAAGCCAGGCACGCACCAACGCCCGGTGAATGCACTTCAATGCCGAGGAGGTc                                                                                        >  2:560956/1‑93 (MQ=255)

 acacATCACGCGCAGGTTGCTTAAACCTTCTTCATGCGCAGAAGCCAGGCACGCACCAACGCCCGGTGAATGCACTTCAATGCCGAGGAGGTc                                                                                        <  2:4499199/93‑1 (MQ=255)

 acacATCACGCGCAGGTTGCTTAAACCTTCTTCATGCGCAGAAGCCAGGCACGCACCAACGCCCGGTGAATGCACTTCAATGCCGAGGAGGTc                                                                                        <  2:3800332/93‑1 (MQ=255)

 acacATCACGCGCAGGTTGCTTAAACCTTCTTCATGCGCAGAAGCCAGGCACGCACCAACGCCCGGTGAATGCACTTCAATGCCGAGGAGGTc                                                                                        <  2:2392082/93‑1 (MQ=255)

 acacATCACGCGCAGGTTGCTTAAACCTTCTTCATGCGCAGAAGCCAGGCACGCACCAACGCCCGGTGAATGCACTTCAATGCCGAGGAGGTc                                                                                        >  2:2723403/1‑93 (MQ=255)

 acacATCACGCGCAGGTTGCTTAAACCTTCTTCATGCGCAGAAGCCAGGCACGCACCAACGCCCGGTGAATGCACTTCAATGCCGAGGAGGTc                                                                                        >  1:196232/1‑93 (MQ=255)

  cacaTCACGCGCAGGTTGCTTAAACCTTCTTCATGCGCAGAAGCCAGGCACGCACCAACGCCCGGTGAATGCACTTCAATGCCGAGGAGGTcc                                                                                       >  2:1045140/1‑93 (MQ=255)

    caTCACGCGCAGGTTGCTTAAACCTTCTTCATGCGCAGAAGCCAGGCACGCACCAACGCCCGGTGAATGCACTTCAATGCCGAGGAGGTCCTg                                                                                     >  2:2462464/1‑93 (MQ=255)

     aTCACGCGCAGGTTGCTTAAACCTTCTTCATGCGCAGAAGCCAGGCACGCACCAACGCCCGGTGAATGCACTTCAATGCCGAGGAGGTCCTGc                                                                                    <  1:1095398/93‑1 (MQ=255)

     aTCACGCGCAGGTTGCTTAAACCTTCTTCATGCGCAGAAGCCAGGCACGCACCAACGCCCGGTGAATGCACTTCAATGCCGAGGAGGTCCTGc                                                                                    >  1:1891956/1‑93 (MQ=255)

       cACGCGCAGGTTGCTTAAACCTTCTTCATGCGCAGAAGCCAGGCACGCACCAACGCCCGGTGAATGCACTTCAATGCCGAGGAGGTCCTGCTc                                                                                  <  2:3184852/93‑1 (MQ=255)

        aCGCGCAGGTTGCTTAAACCTTCTTCATGCGCAGAAGCCAGGCACGCACCAACGCCCGGTGAATGCACTTCAATGCCGAGGAGGTCCTGCTCa                                                                                 <  1:3210910/93‑1 (MQ=255)

        aCGCGCAGGTTGCTTAAACCTTCTTCATGCGCAGAAGCCAGGCACGCACCAACGCCCGGTGAATGCACTTCAATGCCGAGGAGGTCCTGCTCa                                                                                 <  2:2317078/93‑1 (MQ=255)

        aCGCGCAGGTTGCTTAAACCTTCTTCATGCGCAGAAGCCAGGCACGCACCAACGCCCGGTGAATGCACTTCAATGCCGAGGAGGTCCTGCTCa                                                                                 >  2:2979669/1‑93 (MQ=255)

         cgcgCAGGTTGCTTAAACCTTCTTCATGCGCAGAAGCCAGGCACGCACCAACGCCCGGTGAATGCACTTCAATGCCGAGGAGGTCCTGCTCAg                                                                                >  1:1913143/1‑93 (MQ=255)

          gcgcAGGTTGCTTAAACCTTCTTCATGCGCAGAAGCCAGGCACGCACCAACGCCCGGTGAATGCACTTCAATGCCGAGGAGGTCCTGCTCAgg                                                                               <  1:833176/93‑1 (MQ=255)

            gcAGGTTGCTTAAACCTTCTTCATGCGCAGAAGCCAGGCACGCACCAACGCCCGGTGAATGCACTTCAATGCCGAGGAGGTCCTGCTCAGGGc                                                                             >  2:3661043/1‑93 (MQ=255)

            gcAGGTTGCTTAAACCTTCTTCATGCGCAGAAGCCAGGCACGCACCAACGCCCGGTGAATGCACTTCAATGCCGAGGAGGTCCTGCTCAGGGc                                                                             >  1:3069466/1‑93 (MQ=255)

             cAGGTTGCTTAAACCTTCTTCATGCGCAGAAGCCAGGCACGCACCAACGCCCGGTGAATGCACTTCAATGCCGAGGAGGTCCTGCTCAGGGCg                                                                            >  2:206536/1‑93 (MQ=255)

             cAGGTTGCTTAAACCTTCTTCATGCGCAGAAGCCAGGCACGCACCAACGCCCGGTGAATGCAATTCAATGCCGAGGAGGTCCTGCTCAgggtg                                                                            <  2:2285663/93‑3 (MQ=255)

                 ttGCTTAAACCTTCTTCATGCGCAGAAGCCAGGCACGCACCAACGCCCGGTGAATGCACTTCAATGCCGAGGAGGTCCTGCTCAGGGCGATCt                                                                        <  2:3773727/93‑1 (MQ=255)

                 ttGCTTAAACCTTCTTCATGCGCAGAAGCCAGGCACGCACCAACGCCCGGTGAATGCACTTCAATGCCGAGGAGGTCCTGCTCAGGGCGATCt                                                                        <  1:1446860/93‑1 (MQ=255)

                 ttGCTTAAACCTTCTTCATGCGCAGAAGCCAGGCACGCACCAACGCCCGGTGAATGCACTTCAATGCCGAGGAGGTCCTGCTCAGGGCGATCt                                                                        >  2:3922576/1‑93 (MQ=255)

                 ttGCTTAAACCTTCTTCATGCGCAGAAGCCAGGCACGCACCAACGCCCGGTGAATGCACTTCAATGCCGAGGAGGTCCTGCTCAGGGCGATCt                                                                        <  2:789034/93‑1 (MQ=255)

                     ttAAACCTTCTTCATGCGCAGAAGCCAGGCACGCACCAACGCCCGGTGAATGCACTTCAATGCCGAGGAGGTCCTGCTCAGGGCGATCTTTAg                                                                    >  1:2490732/1‑93 (MQ=255)

                     ttAAACCTTCTTCATGCGCAGAAGCCAGGCACGCACCAACGCCCGGTGAATGCACTTCAATGCCGAGGAGGTCCTGCTCAGGGCGATCTTTAg                                                                    <  1:572727/93‑1 (MQ=255)

                     ttAAACCTTCTTCATGCGCAGAAGCCAGGCACGCACCAACGCCCGGTGAATGCACTTCAATGCCGAGGAGGTCCTGCTCAGGGCGATCTTTAg                                                                    >  2:2198283/1‑93 (MQ=255)

                     ttAAACCTTCTTCATGCGCAGAAGCCAGGCACGCACCAACGCCCGGTGAATGCACTTCAATGCCGAGGAGGTCCTGCTCAGGGCGATCTTTAg                                                                    <  1:2852717/93‑1 (MQ=255)

                     ttAAACCTTCTTCATGCGCAGAAGCCAGGCACGCACCAACGCCCGGTGAATGCACTTCAATGCCGAGGAGGTCCTGCTCAGGGCGATCTTTAg                                                                    >  1:176601/1‑93 (MQ=255)

                      tAAACCTTCTTCATGCGCAGAAGCCAGGCACGCACCAACGCCCGGTGAATGCACTTCAATGCCGAGGAGGTCCTGCTCAGGGCGATCTTTAGc                                                                   >  2:1781600/1‑93 (MQ=255)

                      tAAACCTTCTTCATGCGCAGAAGCCAGGCACGCACCAACGCCCGGTGAATGCACTTCAATGCCGAGGAGGTCCTGCTCAGGGCGATCTTTAGc                                                                   >  1:3855844/1‑93 (MQ=255)

                        aaCCTTCTTCATGCGCAGAAGCCAGGCACGCACCAACGCCCGGTGAATGCACTTCAATGCCGAGGAGGTCCTGCTCAGGGCGATCTTTAGCCa                                                                 >  1:4181298/1‑93 (MQ=255)

                         aCCTTCTTCATGCGCAGAAGCCAGGCACGCACCACCGCCCGGTGAATGCACTTCAATGCCGAGGAGGTCCTGCTCAGGGCGATCTTTAGCCAt                                                                <  1:1042333/93‑1 (MQ=255)

                         aCCTTCTTCATGCGCAGAAGCCAGGCACGCACCAACGCCCGGTGAATGCACTTCAATGCCGAGGAGGTCCTGCTCAGGGCGATCTTTAGCCAt                                                                >  2:1877944/1‑93 (MQ=255)

                          ccTTCTTCATGCGCAGAAGCCAGGCACGCACCAACGCCCGGTGAATGCACTTCAATGCCGAGGAGGTCCTGCTCAGGGCGATCTTTAGCCAtt                                                               <  1:3324504/93‑1 (MQ=255)

                          ccTTCTTCATGCGCAGAAGCCAGGCACGCACCAACGCCCGGTGAATGCACTTCAATGCCGAGGAGGTCCTGCTCAGGGCGATCTTTAGCCAtt                                                               >  1:2746741/1‑93 (MQ=255)

                          ccTTCTTCATGCGCAGAAGCCAGGCACGCACCAACGCCCGGTGAATGCACTTCAATGCCGAGGAGGTCCTGCTCAGGGCGATCTTTAGCCAtt                                                               >  2:3965809/1‑93 (MQ=255)

                          ccTTCTTCATGCGCAGAAGCCAGGCACGCACCAACGCCCGGTGAATGCACTTCAATGCCGAGGAGGTCCTGCTCAGGGCGATCTTTAGCCAtt                                                               >  1:1348381/1‑93 (MQ=255)

                           cttcttCATGCGCAGAAGCCAGGCACGCACCAACGCCCGGTGAATGCACTTCAATGCCGAGGAGGTCCTGCTCAGGGCGATCTTTAGCCATTg                                                              >  1:1185853/1‑93 (MQ=255)

                           cttcttCATGCGCAGAAGCCAGGCACGCACCAACGCCCGGTGAATGCACTTCAATGCCGAGGAGGTCCTGCTCAGGGCGATCTTTAGCCATTg                                                              >  1:30971/1‑93 (MQ=255)

                           cttcttCATGCGCAGAAGCCAGGCACGCACCAACGCCCGGTGAATGCACTTCAATGCCGAGGAGGTCCTGCTCAGGGCGATCTTTAGCCATTg                                                              <  2:3962785/93‑1 (MQ=255)

                             tcttcATGCGCAGAAGCCAGGCACGCACCAACGCCCGGTGAATGCACTTCAATGCCGAGGAGGTCCTGCTCAGGGCGATCTTTAGCCATTGcc                                                            <  1:4623528/93‑1 (MQ=255)

                              cttcATGCGCAGAAGCCAGGCACGCACCAACGCCCGGTGAATGCACTTCAATGCCGAGGAGGGCCTGCTCCGGGCGATCTTTAGCCATTGcca                                                           >  2:429636/1‑93 (MQ=255)

                               ttcATGCGCAGAAGCCAGGCACGCACCAACGCCCGGTGAATGCACTTCAATGCCGAGGAGGTCCTGCTCAGGGCGATCTTTAGCCATTGccac                                                          >  2:2617084/1‑93 (MQ=255)

                               ttcATGCGCAGAAGCCAGGCACGCACCAACGCCCGGTGAATGCACTTCAATGCCGAGGAGGTCCTGCTCAGGGCGATCTTTAGCCATTGccac                                                          >  2:4162563/1‑93 (MQ=255)

                                 cATGCGCAGAAGCCAGGCACGCACCAACGCCCGGTGAATGCACTTCAATGCCGAGGAGGTCCTGCTCAGGGCGATCTTTAGCCATTGccacca                                                        <  2:1995499/93‑1 (MQ=255)

                                   tGCGCAGAAGCCAGGCACGCACCAACGCCCGGTGAATGCACTTCAATGCCGAGGAGGTCCTGCTCAGGGCGATCTTTAGCCATTGCCACCAGc                                                      >  1:657146/1‑93 (MQ=255)

                                     cgcAGAAGCCAGGCACGCACCAACGCCCGGTGAATGCACTTCAATGCCGAGGAGGCCCTGCTCAGGGCGATCTTTAGCCATTGCCACCAGCgc                                                    >  2:1931172/1‑92 (MQ=255)

                                      gcAGAAGCCAGGCACGCACCAACGCCCGGTGAATGCACTTCAATGCCGAGGAGGTCCTGCTCAGGGCGATCTTTAGCCATTGCCACCAGCGAc                                                   >  2:3283777/1‑93 (MQ=255)

                                       cAGAAGCCAGGCACGCACCAACGCCCGGTGAATGCACTTCAATGCCGAGGAGGTCCTGCTCAGGGCGATCTTTAGCCATTGCCACCAGCGACg                                                  >  1:2570793/1‑93 (MQ=255)

                                        aGAAGCCAGGCACGCACCAACGCCCGGTGAATGCACTTCAATGCCGAGGAGGTCCTGCTCAGGGCGATCTTTAGCCATTGCCACCAGCGACGc                                                 <  1:987891/93‑1 (MQ=255)

                                         gAAGCCAGGCACGCACCAACGCCCGGTGAATGCACTTCAATGCCGAGGAGGTCCTGCTCAGGGCGATCTTTAGCCATTGCCACCAGCGACGcc                                                >  1:2116303/1‑93 (MQ=255)

                                         gAAGCCAGGCACGCACCAACGCCCGGTGAATGCACTTCAATGCCGAGGAGGTCCTGCTCAGGGCGATCTTTAGCCATTGCCACCAGCGACGcc                                                >  1:1023749/1‑93 (MQ=255)

                                         gAAGCCAGGCACGCACCAACGCCCGGTGAATGCACTTCAATGCCGAGGAGGTCCTGCTCAGGGCGATCTTTAGCCATTGCCACCAGCGACGcc                                                >  2:1796425/1‑93 (MQ=255)

                                           aGCCAGGCACGCACCAACGCCCGGTGAATGCACTTCAATGCCGAGGAGGTCCTGCTCAGGGCGATCTTTAGCCATTGCCACCAGCGACGcccc                                              >  1:1458676/1‑93 (MQ=255)

                                            gCCAGGCACGCACCAACGCCCGGTGAATGCACTTCAATGCCGAGGAGGTCCTGCTCAGGGCGATCTTTAGCCATTGCCACCAGCGACGccccc                                             >  1:610505/1‑93 (MQ=255)

                                            gCCAGGCACGCACCAACGCCCGGTGAATGCACTTCAATGCCGAGGAGGTCCTGCTCAGGGCGATCTTTAGCCATTGCCACCAGCGACGccccc                                             >  1:1190927/1‑93 (MQ=255)

                                             ccAGGCACGCACCAACGCCCGGTGAATGCACTTCAATGCCGAGGAGGTCCTGCTCAGGGCGATCTTTAGCCATTGCCACCAGCGACGCCCCCa                                            <  1:3072810/93‑1 (MQ=255)

                                             ccAGGCACGCACCAACGCCCGGTGAATGCACTTCAATGCCGAGGAGGTCCTGCTCAGGGCGATCTTTAGCCATTGCCACCAGCGACGCCCCCa                                            <  2:468077/93‑1 (MQ=255)

                                             ccAGGCACGCACCAACGCCCGGTGAATGCACTTCAATGCCGAGGAGGTCCTGCTCAGGGCGATCTTTAGCCATTGCCACCAGCGACGCCCCCa                                            <  1:2382847/93‑1 (MQ=255)

                                             ccAGGCACGCACCAACGCCCGGTGAATGCACTTCAATGCCGAGGAGGTCCTGCTCAGGGCGATCTTTAGCCATTGCCACCAGCGACGCCCCCa                                            <  1:3871509/93‑1 (MQ=255)

                                              cAGGCACGCACCAACGCCCGGTGAATGCACTTCAATGCCGAGGAGGTCCTGCTCAGGGCGATCTTTAGCCATTGCCACCAGCGACGCCCCCAt                                           >  2:3667072/1‑93 (MQ=255)

                                               aGGCACGCACCAACGCCCGGTGAATGCACTTCAATGCCGAGGAGGTCCTGCTCAGGGCGATCTTTAGCCATTGCCACCAGCGACGCCCCCATg                                          >  2:749391/1‑93 (MQ=255)

                                                ggCACGCACCAACACCCGGTGGATGCACTTCAATGCCGAGGACGCCCTGCTCAGCGCGATCTTTAGCCATTGCCACCAGCGACGCCCCCATGc                                         >  2:1561989/1‑93 (MQ=255)

                                                 gcacgcacCAACGCCCGGTGAATGCACTTCAATGCCGAGGAGGTCCTGCTCAGGGCGATCTTTAGCCATTGCCACCAGCGACGCCCCCATGcc                                        <  2:4431712/93‑1 (MQ=255)

                                                    cgcacCAACGCCCGGTGAATGCACTTCAATGCCGAGGAGGTCCTGCTCAGGGCGATCTTTAGCCATTGCCACCAGCGACGCCCCCATGCCaaa                                     <  2:3137210/93‑1 (MQ=255)

                                                      cacCAACGCCCGGTGAATGCACTTCAATGCCGAGGAGGTCCTGCTCAGGGCGATCTTTAGCCATTGCCACCAGCGACGCCCCCATGCCAAAAc                                   >  2:581447/1‑93 (MQ=255)

                                                         cAACGCCCGGTGAATGCACTTCAATGCCGAGGAGGTCCTGCTCAGGGCGATCTTTAGCCATTGCCACCAGCGACGCCCCCATGCCAAAACCaa                                >  2:1523692/1‑93 (MQ=255)

                                                         cAACGCCCGGTGAATGCACTTCAATGCCGAGGAGGTCCTGCTCAGGGCGATCTTTAGCCATTGCCACCAGCGACGCCCCCATGCCAAAACCaa                                <  1:1650488/93‑1 (MQ=255)

                                                           aCGCCCGGTGAATGCACTTCAATGCCGAGGAGGTCCTGCTCAGGGCGATCTTTAGCCATTGCCACCAGCGACGCCCCCATGCCAAAACCAAtc                              >  2:217613/1‑93 (MQ=255)

                                                           aCGCCCGGTGAATGCACTTCAATGCCGAGGAGGTCCTGCTCAGGGCGATCTTTAGCCATTGCCACCAGCGACGCCCCCATGCCAAAACCAAtc                              >  2:3443844/1‑93 (MQ=255)

                                                            cGCCCGGTGAATGCACTTCAATGCCGAGGAGGTCCTGCTCAGGGCGATCTTTAGCCATTGCCACCAGCGACGCCCCCATGCCAAAACCAAtct                             <  1:3187222/93‑1 (MQ=255)

                                                             gCCCGGTGAATGCACTTCAATGCCGAGGAGGTCCTGCTCAGGGCGATCTTTAGCCATTGCCACCAGCGACGCCCCCATGCCAAAACCAAtctc                            <  2:4076838/93‑1 (MQ=255)

                                                               ccGGTGAATGCACTTCAATGCCGAGGAGGTCCTGCTCAGGGCGATCTTTAGCCATTGCCACCAGCGACGCCCCCATGCCAAAACCAATCTCaa                          <  2:3403612/93‑1 (MQ=255)

                                                               ccGGTGAATGCACTTCAATGCCGAGGAGGTCCTGCTCAGGGCGATCTTTAGCCATTGCCACCAGCGACGCCCCCATGCCAAAACCAATCTCaa                          <  2:1449114/93‑1 (MQ=255)

                                                               ccGGTGAATGCACTTCAATGCCGAGGAGGTCCTGCTCAGGGCGATCTTTAGCCATTGCCACCAGCGACGCCCCCATGCCAAAACCAATCTCaa                          <  2:3682994/93‑1 (MQ=255)

                                                               ccGGTGAATGCACTTCAATGCCGAGGAGGTCCTGCTCAGGGCGATCTTTAGCCATTGCCACCAGCGACGCCCCCATGCCAAAACCAATCTCaa                          <  2:2033719/93‑1 (MQ=255)

                                                               ccGGTGAATGCACTTCAATGCCGAGGAGGTCCTGCTCAGGGCGATCTTTAGCCATTGCCACCAGCGACGCCCCCATGCCAAAACCAATCTCaa                          >  2:1990953/1‑93 (MQ=255)

                                                               ccGGTGAATGCACTTCAATGCCGAGGAGGTCCTGCTCAGGGCGATCTTGAGCCATTGCCACCAGCGACGCCCCCATGCCAAAACCAATCTCaa                          <  1:3380185/93‑1 (MQ=255)

                                                                cGGTGAATGCACTTCAATGCCGAGGAGGTCCTGCTCAGGGCGATCTTTAGCCATTGCCACCAGCGACGCCCCCATGCCAAAACCAATCTCAAg                         >  1:3006724/1‑93 (MQ=255)

                                                                 ggTGAATGCACTTCAATGCCGAGGAGGTCCTGCTCAGGGCGATCTTTAGCCATTGCCACCAGCGACGCCCCCATGCCAAAACCAATCTCAAGc                        >  2:3130810/1‑93 (MQ=255)

                                                                 ggTGAATGCACTTCAATGCCGAGGAGGTCCTGCTCAGGGCGATCTTTAGCCATTGCCACCAGCGACGCCCCCATGCCAAAACCAATCTCAAGc                        <  2:2759472/93‑1 (MQ=255)

                                                                  gTGAATGCACTTCAATGCCGAGGAGGTCCTGCTCAGGGCGATCTTTAGCCATTGCCACCAGCGACGCCCCCATGCCAAAACCAATCTCAAGCg                       >  2:2639163/1‑93 (MQ=255)

                                                                    gAATGCACTTCAATGCCGAGGAGGTCCTGCTCAGGGCGATCTTTAGCCATTGCCACCAGCGACGCCCCCATGCCAAAACCAATCTCAAGCGTc                     <  1:3480127/93‑1 (MQ=255)

                                                                      aTGCACTTCAATGCCGAGGAGGTCCTGCTCAGGGCGATCTTTAGCCATTGCCACCAGCGACGCCCCCATGCCAAAACCAATCTCAAGCGTCAc                   <  2:3903516/93‑1 (MQ=255)

                                                                       tGCACTTCAATGCCGAGGAGGTCCTGCTCAGGGCGATCTTTAGCCATTGCCACCAGCGACGCCCCCATGCCAAAACCAATCTCAAGCGTCAcc                  >  1:1782119/1‑93 (MQ=255)

                                                                       tGCACTTCAATGCCGAGGAGGTCCTGCTCAGGGCGATCTTTAGCCATTGCCACCAGCGACGCCCCCATGCCAAAACCAATCTCAAGCGTCAcc                  <  1:3683821/93‑1 (MQ=255)

                                                                           cTTCAATGCCGAGGAGGTCCTGCTCAGGGCGATCTTTAGCCATTGCCACCAGCGACGCCCCCATGCCACAACCAATCTCAAGCGTCACCGgcg              >  2:1280122/1‑93 (MQ=255)

                                                                           cTTCAATGCCGAGGAGGTCCTGCTCAGGGCGATCTTTAGCCATTGCCACCAGCGACGCCCCCATGCCAAAACCAATCTCAAGCGTCACCGgcg              >  2:3340819/1‑93 (MQ=255)

                                                                            ttCAATGCCGAGGAGGTCCTGCTCAGGGCGATCTTTAGCCATTGCCACCAGCGACGCCCCCATGCCAAAACCAATCTCAAGCGTCACCGgcgc             <  1:523045/93‑1 (MQ=255)

                                                                            ttCAATGCCGAGGAGGTCCTGCTCAGGGCGATCTTTAGCCATTGCCACCAGCGACGCCCCCATGCCAAAACCAATCTCAAGCGTCACCGgcgc             <  1:4074236/93‑1 (MQ=255)

                                                                             tCAATGCCGAGGAGGTCCTGCTCAGGGCGATCTTTAGCCATTGCCACCAGCGACGCCCCCATGCCAAAACCAATCTCAAGCGTCACCGGCGCt            <  2:2117208/93‑1 (MQ=255)

                                                                               aaTGCCGAGGAGGTCCTGCTCAGGGCGATCTTTAGCCATTGCCACCAGCGACGCCCCCATGCCAAAACCAATCTCAAGCGTCACCGGCGCTTc          <  2:1082625/93‑1 (MQ=255)

                                                                               aaTGCCGAGGAGGTCCTGCTCAGGGCGATCTTTAGCCATTGCCACCAGCGACGCCCCCATGCCAAAACCAATCTCAAGCGTCACCGGCGCTTc          >  2:4515393/1‑93 (MQ=255)

                                                                                aTGCCGAGGAGGTCCTGCTCAGGGCGATCTTTAGCCATTGCCACCAGCGACGCCCCCATGCCAAAACCAATCTCAAGCGTCACCGGCGCTTCa         >  1:3821168/1‑93 (MQ=255)

                                                                                aTGCCGAGGAGGTCCTGCTCAGGGCGATCTTTAGCCATTGCCACCAGCGACGCCCCCATGCCAAAACCAATCTCAAGCGTCACCGGCGCTTCa         <  2:1777049/93‑1 (MQ=255)

                                                                                 tGCCGAGGAGGTCCTGCTCAGGGCGATCTTTAGCCATTGCCACCAGCGACGCCCCCATGCCAAAACCAATCTCAAGCGTCACCGGCGCTTCAc        <  2:1658356/93‑1 (MQ=255)

                                                                                    cGAGGAGGTCCTGCTCAGGGCGATCTTTAGCCATTGCCACCAGCGACGCCCCCATGCCAAAACCAATCTCAAGCGTCACCGGCGCTTCACGGc     >  1:1507181/1‑93 (MQ=255)

                                                                                       ggAGGTCCTGCTCAGGGCGATCTTTAGCCATTGCCACCAGCGACGCCCCCATGCCAAAACCAATCTCAAGCGTCACCGGCGCTTCACGGCCaa  >  2:1264480/1‑93 (MQ=255)

GACACATCACGCGCAGGTTGCTTAAACCTTCTTCATGCGCAGAAGCCAGGCACGCACCAACGCCCGGTGAATGCACTTCAATGCCGAGGAAGTCCTGCTCAGGGCGATCTTTAGCCATTGCCACCAGCGACGCCCCCATGCCAAAACCAATCTCAAGCGTCACCGGCGCTTCACGGCCAA  >  NC_000913/3100513‑3100692

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 18 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`