BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,125,293	T→C	G248G (GGA→GGG)	glcD ←	glycolate oxidase subunit D

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,125,293	0	T	C	100.0%	465.6 / NA	150	G248G (GGA→GGG)	glcD	glycolate oxidase subunit D
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (77/73); total (77/73)

Only 100 of 157 total aligned reads displayed.

AAAATCTTCCGCCGCGCGGATCGACAGGTTATCCATCATCTCCAGCCCGCCGGGGATAATGCCATTGGCGATGATGTCACCAACCGCAAGTCCGGCTTTTTCTACCGAGTCAAAGCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGTTTTACCGTCACTTCGGTGGTCAC  >  NC_000913/3125203‑3125382

agaaTCTCCCGCCGCGCGGATCGACAGGTTATCCATCATCTCCCGCCCGCCGGGGATAATGCCATTGGCGATGATGTCACCAACCGCAAGccc                                                                                         <  2:714230/91‑1 (MQ=255)

aaaaTCTTCCGCCGCGCGGATCGACAGGTTATCCATCATCTCCAGCCCGCCGGGGATAATGCCATTGGCGATGATGTCACCAACCGCAAGccc                                                                                         >  2:1302737/1‑93 (MQ=255)

  aaTCTTCCGCCGCGCGGATCGACAGGTTATCCATCATCTCCAGCCCGCCGTGGATAATGCCATTGGCGATGATGTCACCAACCACAAGCCCgg                                                                                       >  2:3873386/1‑93 (MQ=255)

  aaTCTTCCGCCGCGCGGATCGACAGGTTATCCATCATCTCCAGCCCGCCGGGGATAATGCCATTGGCGATGATGTCACCAACCGCAAGCCCgg                                                                                       >  1:4643740/1‑93 (MQ=255)

   aTCTTCCGCCGCGCGGATCGACAGGTTATCCATCATCTCCAGCCCGCCGGGGATAATGCCATTGGCGATGATGTCACCAACCGCAAGCCCGGc                                                                                      <  2:1468885/93‑1 (MQ=255)

    tCTTCCGCCGCGCGGATCGACAGGTTATCCATCATCTCCAGCCCGCCGGGGATAATGCCATTGGCGATGATGTCACCAACCGCAAGCCCGGCt                                                                                     >  2:3662418/1‑93 (MQ=255)

    tCTTCCGCCGCGCGGATCGACAGGTTATCCATCATCTCCAGCCCGCCGGGGATAATGCCATTGGCGATGATGTCACCAACCGCAAGCCCGGCt                                                                                     >  2:1773350/1‑93 (MQ=255)

         cgccgcGCGGATCGACAGGTTATCCATCATCTCCAGCCCGCCGGGGATAATGCCATTGGCGATGATGTCACCAACCGCAAGCCCGGCTTTTTc                                                                                <  2:2601912/93‑1 (MQ=255)

         cgccgcGCGGATCGACAGGTTATCCATCATCTCCAGCCCGCCGGGGATAATGCCATTGGCGATGATGTCACCAACCGCAAGCCCGGCTTTTTc                                                                                >  2:4478566/1‑93 (MQ=255)

          gccgcGCGGATCGACAGGTTATCCATCATCTCCAGCCCGCCGGGGATAATGCCATTGGCGATGATGTCACCAACCGCAAGCCCGGCTTTTTCt                                                                               <  2:4643145/93‑1 (MQ=255)

            cgcgcgGATCGACAGGTTATCCATCATCTCCAGCCCGCCGGGGATAATGCCATTGGCGATGATGTCACCAACCGCAAGCCCGGCTTTTTCTAc                                                                             <  1:1228745/93‑1 (MQ=255)

            cgcgcgGATCGACAGGTTATCCATCATCTCCAGCCCGCCGGGGATAATGCCATTGGCGATGATGTCACCAACCGCAAGCCCGGCTTTTTCTAc                                                                             >  2:4268872/1‑93 (MQ=255)

              cgcgGATCGACAGGTTATCCATCATCTCCAGCCCGCCGGGGATAATGCCATTGGCGATGATGTCACCAACCGCAAGCCCGGCTTTTTCTACCg                                                                           <  1:3115363/93‑1 (MQ=255)

              cgcgGATCGACAGGTTATCCATCATCTCCAGCCCGCCGGGGATAATGCCATTGGCGATGATGTCACCAACCGCAAGCCCGGCTTTTTCTACCg                                                                           <  1:3281143/93‑1 (MQ=255)

               gcgGATCGACAGGTTATCCATCATCTCCAGCCCGCCGGGGATAATGCCATTGGCGATGATGTCACCAACCGCAAGCCCGGCTTTTTCTACCGa                                                                          <  1:1331254/93‑1 (MQ=255)

                 ggATCGACAGGTTATCCATCATCTCCAGCCCGCCGGGGATAATGCCATTGGCGATGATGTCACCAACCGCAAGCCCGGCTTTTTCTACCGCGt                                                                        >  1:2085309/1‑93 (MQ=255)

                 ggATCGACAGGTTATCCATCATCTCCAGCCCGCCGGGGATAATGCCATTGGCGATGATGTCACCAACCGCAAGCCCGGCTTTTTCTACCGAGt                                                                        >  1:3598851/1‑93 (MQ=255)

                 ggATCGACAGGTTATCCATCATCTCCAGCCCGCCGGGGATAATGCCATTGGCGATGATGTCACCAACCGCAAGCCCGGCTTTTTCTACCGAGt                                                                        >  1:2772003/1‑93 (MQ=255)

                 ggATCGACAGGTTATCCATCATCTCCAGCCCGCCGGGGATAATGCCATTGGCGATGATGTCACCAACCGCAAGCCCGGCTTTTTCTACCGAGt                                                                        <  2:1034746/93‑1 (MQ=255)

                  gATCGACAGGTTATCCATCATCTCCAGCCCGCCGGGGATAATGCCATTGGCGATGATGTCACCAACCGCAAGCCCGGCTTTTTCTACCGAGTc                                                                       <  1:2297640/93‑1 (MQ=255)

                  gATCGACAGGTTATCCATCATCCCCAGCCCGCCGGGGATAATGCCATTGGCGATGATGTCACCAACCGCAAGCCCGGCTTTTTCTACCGAGTc                                                                       <  2:3750097/93‑1 (MQ=255)

                   aTCGACAGGTTATCCATCATCTCCAGCCCGCCGGGGATAATGCCATTGGCGATGATGTCACGAACCGCAAGCCCGGCTTTTTCTACCGAGTCa                                                                      >  1:952530/1‑93 (MQ=255)

                     cGACAGGTTATCCATCATCTCCAGCCCGCAGGTGATAATGCCATTGGCGATGATGTCACCAACCGCAAGCCCGGCTTTTTCTACCGAGTCaaa                                                                    <  1:232207/93‑1 (MQ=255)

                      gACAGGTTATCCATCATCTCCAGCCCGCCGGGGATAATGCCATTGGCGATGATGTCACCAACCGCAAGCCCGGCTTTTTCTACCGAGTCAAAg                                                                   <  2:2437850/93‑1 (MQ=255)

                      gACAGGTTATCCATCATCTCCAGCCCGCCGGGGATAATGCCATTGGCGATGATGTCACCAACCGCAAGCCCGGCTTTTTCTACCGAGTCAAAg                                                                   >  1:2218589/1‑93 (MQ=255)

                      gACAGGTTATCCATCATCTCCAGCCCGCCGGGGATAATGCCATTGGCGATGATGTCACCAACCGCAAGCCCGGCTTTTTCTACCGAGTCAAAg                                                                   <  1:1418797/93‑1 (MQ=255)

                      gACAGGTTATCCATCATCTCCAGCCCGCCGGGGATAATGCCATTGGCGATGATGTCACCAACCGCAAGCCCGGCTTTTTCTACCGAGTCAAAg                                                                   <  1:4303623/93‑1 (MQ=255)

                       aCAGGTTATCCATCATCTCCAGCCCGCCGGGGATAATGCCATTGGCGATGATGTCACCAACCGCAAGCCCGGCTTTTTCTACCGAGTCAAAGc                                                                  <  2:795432/93‑1 (MQ=255)

                        cAGGTTATCCATCATCTCCAGCCCGCCGGGGATAATGCCATTGGCGATGATGTCACCAACCGCAAGCCCGGCTTTTTCTACCGAGTCAAAGCt                                                                 >  1:1535392/1‑93 (MQ=255)

                           gTTATCCATCATCTCCAGCCCGCCGGGGATAATGCCATTGGCGATGATGTCACCAACCGCAAGCCCGGCTTTTTCTACCGAGTCAAAGCTgac                                                              >  2:1559311/1‑91 (MQ=255)

                           gTTATCCATCATCTCCAGCCCGCCGGGGATAATGCCATTGGCGATGATGTCACCAACCGCAAGCCCGGCTTTTTCTACCGAGTCAAAGCTGGc                                                              >  2:3397089/1‑93 (MQ=255)

                            ttATCCATCATCTCCAGCCCGCCGGGGATAATGCCATTGGCGATGATGTCACCAACCGCAAGCCCGGCTTTTTCTACCGAGTCAAAGCTGGCt                                                             <  2:4355531/93‑1 (MQ=255)

                            ttATCCATCATCTCCAGCCCGCCGGGGATAATGCCATTGGCGATGATGTCACCAACCGCAAGCCCGGCTTTTTCTACCGAGTCAAAGCTGGCt                                                             <  1:3752414/93‑1 (MQ=255)

                             tATCCATCATCTCCAGCCCGCCGGGGATAATGCCATTGGCGATGATGTCACCAACCGCAAGCCCGGCTTTTTCTACCGAGTCAAAGCTGGCTa                                                            >  2:1355199/1‑93 (MQ=255)

                              aTCCATCATCTCCAGCCCGCCGGGGATAATGCCATTGGCGATGATGTCACCAACCGCAAGCCCGGCTTTTTCTACCGAGTCAAAGCTGGCTaa                                                           >  1:4061682/1‑93 (MQ=255)

                              aTCCATCATCTCCAGCCCGCCGGGGATAATGCCATTGGCGATGATGTCACCAACCGCAAGCCCGGCTTTTTCTACCGAGTCAAAGCTGGCTaa                                                           <  2:189285/93‑1 (MQ=255)

                               tCCATCATCTCCAGCCCGCCGGGGATAATGCCATTGGCGATGATGTCACCAACCGCAAGCCCGGCTTTTTCTACCGAGTCAAAGCTGGCTAAc                                                          <  1:4337568/93‑1 (MQ=255)

                               tCCATCATCTCCAGCCCGCCGGGGATAATGCCATTGGCGATGATGTCACCAACCGCAAGCCCGGCTTTTTCTACCGAGTCAAAGCTGGCTAAc                                                          <  1:4677749/93‑1 (MQ=255)

                               tCCATCATCTCCAGCCCGCCGGGGATAATGCCATTGGCGATGATGTCACCAACCGCAAGCCCGGCTTTTTCTACCGAGTCAAAGCTGGCTAAc                                                          >  2:3483813/1‑93 (MQ=255)

                               tCCATCATCTCCAGCCCGCCGGGGATAATGCCATTGGCGATGATGTCACCAACCGCAAGCCCGGCTTTTTCTACCGAGTCAAAGCTGGCTAAc                                                          >  2:1906782/1‑93 (MQ=255)

                                ccATCATCTCCAGCCCGCCGGGGATAATGCCATTGGCGATGATGTCACCAACCGCAAGCCCGGCTTTTTCTACCGAGTCAAAGCTGGCTAACa                                                         <  1:949677/93‑1 (MQ=255)

                                   tcatcTCCAGCCCGCCGGGGATAATGCCATTGGCGATGATGTCACCAACCGCAAGCCCGGCTTTTTCTACCGAGTCAAAGCTGGCTAACAGaa                                                      >  1:3049091/1‑93 (MQ=255)

                                   tcatcTCCAGCCCGCCGGGGATAATGCCATTGGCGATGATGTCACCAACCGCAAGCCCGGCTTTTTCTACCGAGTCAAAGCTGGCTAACAGaa                                                      >  2:4564582/1‑93 (MQ=255)

                                     atcTCCAGCCCGCCGGGGATAATGCCATTGGCGATGATGTCACCAACCGCAAGCCCGGCTTTTTCTACCGAGTCAAAGCTGGCTAACAGAAcc                                                    >  2:468907/1‑93 (MQ=255)

                                     atcTCCAGCCCGCCGGGGATAATGCCATTGGCGATGATGTCACCAACCGCAAGCCCGGCTTTTTCTACCGAGTCAAAGCTGGCTAACAGAAcc                                                    <  2:225081/93‑1 (MQ=255)

                                     atcTCCAGCCCGCCGGGGATAATGCCATTGGCGATGATGTCACCAACCGCAAGCCCGGCTTTTTCTACCGAGTCAAAGCTGGCTAACAGAAcc                                                    <  1:2768480/93‑1 (MQ=255)

                                     atcTCCAGCCCGCCGGGGATAATGCCATTGGCGATGATGTCACCAACCGCAAGCCCGGCTTTTTCTACCGAGTCAAAGCTGGCTAACAGAAcc                                                    <  1:3924125/93‑1 (MQ=255)

                                      tctcCAGCCCGCCGGGGATAATGCCATTGGCGATGATGTCACCAACCGCAAGCCCGGCTTTTTCTACCGAGTCAAAGCTGGCTAACAGAAcct                                                   >  1:1936528/1‑92 (MQ=255)

                                      tctcCAGCCCGCCGGGGATAATGCCATTGGCGATGATGTCACCAACCGCAAGCCCGGCTTTTTCTACCGAGTCAAAGCTGGCTAACAGAAccc                                                   >  2:3207880/1‑93 (MQ=255)

                                         ccAGCCCGCCGGGGATAATGCCATTGGCGATGATGTCACCAACCGCAAGCCCGGCTTTTTCTACCGAGTCAAAGCTGGCTAACAGAACCcgcc                                                >  1:3476864/1‑92 (MQ=255)

                                          cAGCCCGCCGGGGATAATGCCATTGGCGATGATGTCACCAACCGCAAGCCCGGCTTTTTCTACCGAGTCAAAGCTGGCTAACAGAACCCgcgc                                               <  2:2625699/93‑1 (MQ=255)

                                           aGCCCGCCGGGGATAATGCCATTGGCGATGATGTCACCAACCGCAAGCCCGGCTTTTTCTACCGAGTCAACGCTGGCTAACAGAACCCGCGcc                                              >  2:1814846/1‑93 (MQ=255)

                                           aGCCCGCCGGGGATAATGCCATTGGCGATGATGTCACCAACCGCAAGCCCGGCTTTTTCTACCGAGTCAAAGCTGGCTAACAGAACCCGCGcc                                              >  1:2845215/1‑93 (MQ=255)

                                            gCCCGCCGGGGATAATGCCATTGGCGATGATGTCACCAACCGCAAGCCCGGCTTTTTCTACCGAGTCAAAGCTGGCTAACAGAACCCGCGCCa                                             >  2:877548/1‑93 (MQ=255)

                                              ccgccgGGGATAATGCCATTGGCGATGATGTCACCAACCGCAAGCCCGGCTTTTTCTACCGAGTCAAAGCTGGCTAACAGAAGCCGCGCCACg                                           >  2:2122598/1‑93 (MQ=255)

                                               cgccgGGGATAATGCCATTGGCGATGATGTCACCAACCGCAAGCCCGGCTTTTTCTACCGAGTCAAAGCTGGCTAACAGAACCCGCGCCACgg                                          >  2:198995/1‑93 (MQ=255)

                                                 ccgGGGATAATGCCATTGGCGATGATGTCACCAACCGCAAGCCCGGCTTTTTCTACCGAGTCAAAGCTGGCTAACAGAACCCGCGCCACGggc                                        >  2:1358263/1‑93 (MQ=255)

                                                  cgGGGATAATGCCATTGGCGATGATGTCACCAACCGCAAGCCCGGCTTTTTCTACCGAGTCAAAGCTGGCTAACAGAACCCGCGCCACGggcg                                       >  2:1592388/1‑93 (MQ=255)

                                                    gggATAATGCCATTGGCGATGATGTCACCAACCGCAAGCCCGGCTTTTTCTACCGAGTCAAAGCTGGCTAACAGAACCCGCGCCACGggcggc                                     >  2:3944010/1‑93 (MQ=255)

                                                    gggATAATGCCATTGGCGATGATGTCACCAACCGCAAGCCCGGCTTTTTCTACCGAGTCAAAGCTGGCTAACAGAACCCGCGCCACGggcggc                                     <  1:461889/93‑1 (MQ=255)

                                                    gggATAATGCCATTGGCGATGATGTCACCAACCGCAAGCCCGGCTTTTTCTACCGAGTCAAAGCTGGCTAACAGAACCCGCGCCACGggcggc                                     <  1:622667/93‑1 (MQ=255)

                                                    gggATAATGCCATTGGCGATGATGTCACCAACCGCAAGCCCGGCTTTTTCTACCGAGTCAAAGCTGGCTAACAGAACCCGCGCCACGggcggc                                     >  2:2827306/1‑93 (MQ=255)

                                                     ggATAATGCCATTGGCGATGATGTCCCCAACCGCAAGCCCGGCTTTTTCTACCGAGTCAAAGCTGGCTAACAGAACCCGCGCCACGGGCGGCt                                    <  2:1508020/93‑1 (MQ=255)

                                                     ggATAATGCCATTGGCGATGATGTCACCAACCGCAAGCCCGGCTTTTTCTACCGAGTCAAAGCTGGCTAACAGAACCCGCGCCACGGGCGGCt                                    >  1:1204868/1‑93 (MQ=255)

                                                      gATAATGCCATTGGCGATGATGTCACCAACCGCAAGCCCGGCTTTTTCTACCGAGTCAAAGCTGGCTAACAGAACCCGCGCCACGGGCGGCtt                                   >  1:2646712/1‑93 (MQ=255)

                                                      gATAATGCCATTGGCGATGATGTCACCAACCGCAAGCCCGGCTTTTTCTACCGAGTCAAAGCTGGCTAACAGAACCCGCGCCACGGGCGGCtt                                   <  2:194191/93‑1 (MQ=255)

                                                        tAATGCCATTGGCGATGATGTCACCAACCGCAAGCCCGGCTTTTTCTACCGAGTCAAAGCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCg                                 <  2:2079119/93‑1 (MQ=255)

                                                          aTGCCATTGGCGATGATGTCACCAACCGCAAGCCCGGCTTTTTCTACCGAGTCAAAGCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGgc                               >  2:4117989/1‑93 (MQ=255)

                                                          aTGCCATTGGCGATGATGTCACCAACCGCAAGCCCGGCTTTTTCTACCGAGTCAAAGCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGgc                               <  2:2860480/93‑1 (MQ=255)

                                                          aTGCCATTGGCGATGATGTCACCAACCGCAAGCCCGGCTTTTTCTACCGAGTCAAAGCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGgc                               >  2:2166744/1‑93 (MQ=255)

                                                            gCCATTGGCGATGATGTCACCAACCGCAAGCCCGGCTTTTTCTACCGAGTCAAAGCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGgcag                             >  2:3180924/1‑93 (MQ=255)

                                                             ccATTGGCGATGATGTCACCAACCGCAAGCCCGGCTTTTTCTACCGAGTCAAAGCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGgcagc                            <  1:77410/93‑1 (MQ=255)

                                                             ccATTGGCGATGATGTCACCAACCGCAAGCACGGCTTTTTCTACCGAGTCAAAGCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGgcaga                            >  1:2149212/1‑92 (MQ=255)

                                                              cATTGGCGATGATGTCACCAACCGCAAGCCCGGCTTTTTCTACCGAGTCAAAGCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGgcagca                           <  2:2626467/93‑1 (MQ=255)

                                                              cATTGGCGATGATGTCACCAACCGCAAGCCCGGCTTTTTCTACCGAGTCAAAGCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGgcagca                           <  2:3044589/93‑1 (MQ=255)

                                                              cATTGGCGATGATGTCACCAACCGCAAGCCCGGCTTTTTCTACCGAGTCAAAGCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGgcagca                           <  2:2210193/93‑1 (MQ=255)

                                                               aTTGGCGATGATGTCACCAACCGCAAGCCCGGCTTTTTCTACCGAGTCAAAGCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGcagcag                          <  1:2563254/93‑1 (MQ=255)

                                                                ttGGCGATGATGTCACCAACCGCAAGCCCGGCTTTTTCTACCGAGTCAAAGCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGcagcagc                         <  2:560553/93‑2 (MQ=255)

                                                                 tGGCGATGATGTCACCAACCGCAAGCCCGGCTTTTTCTACCGAGTCAAAGCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGcagcagct                        >  2:807333/1‑91 (MQ=255)

                                                                 tGGCGATGATGTCACCAACCGCAAGCCCGGCTTTTTCTACCGAGTCAAAGCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGcagcagct                        <  1:2585083/93‑3 (MQ=255)

                                                                 tGGCGATGATGTCACCAACCGCAAGCCCGGCTTTTTCTACCGAGTCAAAGCTGGCAAACAGAACCCGCGCCACGGGCGGCTTCGGcagcagct                        <  1:3394040/93‑3 (MQ=255)

                                                                  ggCGATGATGTCACCAACCGCAAGCCCGGCTTTTTCTACCGAGTCAAAGCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGctt                       <  1:999127/93‑1 (MQ=255)

                                                                   gCGATGATGTCACCAACCGCAAGCCCGGCTTTTTCTACCGAGTCAAAGCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGcttt                      >  2:2751024/1‑93 (MQ=255)

                                                                   gCGATGATGTCACCAACCGCAAGCCCGGCTTTTTCTACCGAGTCAAAGCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGcttt                      <  2:457429/93‑1 (MQ=255)

                                                                      atgatgTCACCAACCGCAAGCCCGGCTTTTTCTACCGAGTCAAAGCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTAcc                   >  1:2429782/1‑93 (MQ=255)

                                                                        gatgTCACCAACCGCAAGCCCGGCTTTTTCTACCGAGTCAAAGCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACCGt                 >  2:3679346/1‑93 (MQ=255)

                                                                        gatgTCACCAACCGCAAGCCCGGCTTTTTCTACCGAGTCAAAGCTGGCTAACAGAACCCGCGCCACGGGCGGCGTCGGCAGCAGCTTTACCgc                 >  2:3346746/1‑92 (MQ=255)

                                                                         atgTCACCAACCGCAAGCCCGGCTTTTTCTACCGAGTCAAAGCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACCGTc                <  1:415786/93‑1 (MQ=255)

                                                                          tgTCACCAACCGCAAGCCCGGCTTTTTCTACCGAGTCAAAGCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACCGTCa               >  2:67174/1‑93 (MQ=255)

                                                                            tCACCAACCGCAAGCCCGGCTTTTTCTACCGAGTCAAAGCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACCGTCACt             >  1:145859/1‑93 (MQ=255)

                                                                              aCCAACCGCAAGCCCGGCTTTTTCTACCGAGTCAAAGCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACCGTCACTTc           <  2:1578058/93‑1 (MQ=255)

                                                                              aCCAACCGCAAGCCCGGCTTTTTCTACCGAGTCAAAGCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACCGTCACTTc           <  2:4518957/93‑1 (MQ=255)

                                                                                cAACCGGAAGCCCGGCGTTTACTACGGAGTCAAAGCTGGCTAACAGAGCCCTGGCCCCGGGCGGCTTCGGCAGCATCTTTACCGTCTCGTCgg         <  2:3103442/93‑1 (MQ=255)

                                                                                cAACCGCAAGCCCGGCTTTTTCTACCGAGTCAAAGCTGGCTAACAGAGCCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACCGTCACTTCgg         >  1:4612493/1‑93 (MQ=255)

                                                                                 aaCCGCAAGCCCGGCTTTTTCTACCGAGTCAAAGCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACCGTCACTTCggt        >  2:1238909/1‑93 (MQ=255)

                                                                                 aaCCGCAAGCCCGGCTTTTTCTACCGAGTCAAAGCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACCGTCACTTCggt        >  2:1148572/1‑93 (MQ=255)

                                                                                 aaCCGCAAGCCCGGCTTTTTCTACCGAGTCAAAGCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACCGTCACTTCggg        >  2:3753152/1‑92 (MQ=255)

                                                                                  aCCGCAAGCCCGGCTTTTTCTACCGAGTCAAAGCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACCGTCACTTCggtg       <  2:171016/93‑1 (MQ=255)

                                                                                    cGCAAGCCCGGCTTTTTCTACCGAGTCAAAGCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACCGTCACTTCggtggt     <  1:836227/93‑1 (MQ=255)

                                                                                     gCAAGCCCGGCTTTTTCTACCGAGTCAAAGCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACCGTCACTTCGGTGGTc    >  1:3827164/1‑93 (MQ=255)

AAAATCTTCCGCCGCGCGGATCGACAGGTTATCCATCATCTCCAGCCCGCCGGGGATAATGCCATTGGCGATGATGTCACCAACCGCAAGTCCGGCTTTTTCTACCGAGTCAAAGCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGTTTTACCGTCACTTCGGTGGTCAC  >  NC_000913/3125203‑3125382

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 19 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`