BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,125,359	T→C	K226K (AAA→AAG)	glcD ←	glycolate oxidase subunit D

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,125,359	0	T	C	100.0%	441.3 / NA	140	K226K (AAA→AAG)	glcD	glycolate oxidase subunit D
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (69/71); total (69/71)

Only 100 of 158 total aligned reads displayed.

GGCGATGATGTCACCAACCGCAAGTCCGGCTTTTTCTACCGAGTCAAAGCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGTTTTACCGTCACTTCGGTGGTCACGCCGAGCATACCTTCCGATCCGGTGAACAGCGCCAGCAGGTCAAAACCAGGTGAATCCAGCGCGTC  >  NC_000913/3125269‑3125448

ggCGATGATGTCACCAACCGCAAGCCCGGCTTTTTCTACCGAGTCAAAGCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGctt                                                                                         <  2:4543203/93‑1 (MQ=255)

 gCGATGATGTCACCAACCGCAAGCCCGGCTTTTTCTACCGAGTCAAAGCTGGCTAACAGAACCCGCGCCACGGTCGGCTTCGGCAGCAGcttt                                                                                        <  1:2816250/93‑1 (MQ=255)

 gCGATGATGTCACCAACCGCAAGCCCGGCTTTTTCTACCGAGTCAAAGCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGcttt                                                                                        <  2:457429/93‑1 (MQ=255)

    atgatgTCACCAACCGCAAGCCCGGCTTTTTCTACCGAGTCAAAGCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTAcc                                                                                     >  2:740973/1‑93 (MQ=255)

     tgatgTCACCAACCGCAAGCCCGGCTTTTTCTACCGAGTCAAAGCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACCg                                                                                    >  1:3880234/1‑93 (MQ=255)

      gatgTCACCAACCGCAAGCCCGGCTTTTTCTACCGAGTCAAAGCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACCGt                                                                                   >  2:3679346/1‑93 (MQ=255)

      gatgTCACCAACCGCAAGCCCGGCTTTTTCTACCGAGTCAAAGCTGGCTAACAGAACCCGCGCCACGGGCGGCGTCGGCAGCAGCTTTACCgc                                                                                   >  2:3346746/1‑92 (MQ=255)

       atgTCACCAACCGCAAGCCCGGCTTTTTCTACCGAGTCAAAGCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACCGTc                                                                                  <  1:415786/93‑1 (MQ=255)

        tgTCACCAACCGCAAGCCCGGCTTTTTCTACCGAGTCAAAGCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACCGTCa                                                                                 >  2:67174/1‑93 (MQ=255)

          tCACCAACCGCAAGCCCGGCTTTTTCTACCGAGTCAAAGCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACCGTCACt                                                                               >  1:145859/1‑93 (MQ=255)

           cACCAACCGCAAGCCCGGCTTTTTCTACCGAGTCAAAGCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACCGTCACtt                                                                              <  2:62087/93‑1 (MQ=255)

            aCCAACCGCAAGCCCGGCTTTTTCTACCGAGTCAAAGCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACCGTCACTTc                                                                             <  2:1578058/93‑1 (MQ=255)

            aCCAACCGCAAGCCCGGCTTTTTCTACCGAGTCAAAGCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACCGTCACTTc                                                                             <  2:4518957/93‑1 (MQ=255)

             ccAACCGCAAGCCCGGCTTTTTCTACCGAGTCAAAGCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACCGTCACTTCg                                                                            <  1:476329/93‑1 (MQ=255)

              cAACCGGAAGCCCGGCGTTTACTACGGAGTCAAAGCTGGCTAACAGAGCCCTGGCCCCGGGCGGCTTCGGCAGCATCTTTACCGTCTCGTCgg                                                                           <  2:3103442/93‑1 (MQ=255)

               aaCCGCAAGCCCGGCTTTTTCTACCGAGTCAAAGCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACCGTCACTTCggt                                                                          >  2:1238909/1‑93 (MQ=255)

               aaCCGCAAGCCCGGCTTTTTCTACCGAGTCAAAGCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACCGTCACTTCggt                                                                          >  2:1148572/1‑93 (MQ=255)

               aaCCGCAAGCCCGGCTTTTTCTACCGAGTCAAAGCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACCGTCACTTCggg                                                                          >  2:3753152/1‑92 (MQ=255)

                aCCGCAAGCCCGGCTTTTTCTACCGAGTCAAAGCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACCGTCACTTCggtg                                                                         <  2:171016/93‑1 (MQ=255)

                 ccGCAAGCCCGGCTTTTTCTACCGAGTCAAAGCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACCGTCACTTCggtgg                                                                        >  2:2131299/1‑93 (MQ=255)

                  cGCAAGCCCGGCTTTTTCTACCGAGTCAAAGCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACCGTCACTTCggtggt                                                                       <  1:836227/93‑1 (MQ=255)

                  cGCAAGCCCGGCTTTTTCTACCGAGTCAAAGCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACCGTCACTTCggtggt                                                                       >  1:4461976/1‑93 (MQ=255)

                     aaGCCCGGCTTTTTCTACCGAGTCAAAGCTGGCTAACAGAACCCGCGCCACGGTCGGCTTCGGCAGCAGCGTTAACGTCACTTCGGTGGTCAc                                                                    >  2:4262210/1‑93 (MQ=255)

                       gcccGGCTTTTTCTACCGAGTCAAAGCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACCGTCACTTCGGTGGTCACGc                                                                  <  2:1094331/91‑1 (MQ=255)

                       gcccGGCTTTTTCTACCGAGTCAAAGCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACCGTCACTTCGGTGGTCACGc                                                                  >  2:809215/3‑93 (MQ=255)

                        cccGGCTTTTTCTACCGAGTCAAAGCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACCGTCACTTCGGTGGTCACGcc                                                                 <  1:3243087/92‑1 (MQ=255)

                         ccGGCTTTTTCTACCGAGTCAAAGCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACCGTCACTTCGGTGGTCACGCCg                                                                >  2:3705448/1‑93 (MQ=255)

                          cGGCTTTTTCTACCGAGTCAAAGCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACCGTCACTTCGGTGGTCACGCCGa                                                               <  1:4419388/93‑1 (MQ=255)

                          cGGCTTTTTCTACCGAGTCAAAGCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACCGTCACTTCGGTGGTCACGCCGa                                                               >  2:3987096/1‑93 (MQ=255)

                           ggCTTTTTCTACCGAGTCAAAGCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACCGTCACTTCGGTGGTCACGCCGAg                                                              >  1:3037277/1‑93 (MQ=255)

                            gCTTTTTCTACCGAGTCAAAGCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACCGTCACTTCGGTGGTCACGCCGAGc                                                             >  1:4126504/1‑93 (MQ=255)

                            gCTTTTTCTACCGAGTCAAAGCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACCGTCACTTCGGTGGTCACGCCGAGc                                                             >  2:3883098/1‑93 (MQ=255)

                             cTTTTTCTACCGAGTCAAAGCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACCGTCACTTCGGTGGTCACGCCGAGCa                                                            <  1:3759402/93‑1 (MQ=255)

                             cTTTTTCTACCGAGTCAAAGCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACCGTCACTTCGGTGGTCACGCCGAGCa                                                            <  1:3759403/93‑1 (MQ=255)

                             cTTTTTCTACCGAGTCAAAGCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACCGTCACTTCGGTGGTCACGCCGAGCa                                                            >  2:4619996/1‑93 (MQ=255)

                              tttttCTACCGAGTCAAAGCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACCGTCACTTCGGTGGTCACGCCGAGCAt                                                           >  2:2804051/1‑93 (MQ=255)

                              tttttCTACCGAGTCAAAGCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACCGTCACTTCGGTGGTCACGCCGAGCAt                                                           <  2:2660743/93‑1 (MQ=255)

                              tttttCTACCGAGTCAAAGCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACCGTCACTTCGGTGGTCACGCCGAGCAt                                                           <  2:602032/93‑1 (MQ=255)

                              tttttCTACCGAGTCAAAGCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACCGTCACTTCGGTGGTCACGCCGAGCAt                                                           >  2:1795607/1‑93 (MQ=255)

                               ttttCTACCGAGTCAAAGCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACCGTCACTTCGGTGGTCACGCCGAGCATa                                                          >  2:1421131/1‑93 (MQ=255)

                                 ttCTACCGAGTCAAAGCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACCGTCACTTCGGTGGTCACGCCGAGCATAcc                                                        <  1:1766804/93‑1 (MQ=255)

                                 ttCTACCGAGTCAAAGCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACCGTCACTTCGGTGGTCACGCCGAGCATAcc                                                        <  1:3119805/93‑1 (MQ=255)

                                 ttCTACCGAGTCAAAGCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACCGTCACTTCGGTGGTCACGCCGAGCATAcc                                                        <  1:1331721/93‑1 (MQ=255)

                                   cTACCGAGTCAAAGCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACCGTCACTTCGGTGGTCACGCCGAGCACACCtt                                                      >  2:4474981/1‑93 (MQ=255)

                                    tACCGAGTCAAAGCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACCGTCACTTCGGTGGTCACGCCGAGCATACCTTc                                                     >  2:3551392/1‑93 (MQ=255)

                                    tACCGAGTCAAAGCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACCGTCACTTCGGTGGTCACGCCGAGCATACCTTc                                                     >  2:1583024/1‑93 (MQ=255)

                                    tACCGAGTCAAAGCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACCGTCACTTCGGTGGTCACGCCGAGCATACCTTc                                                     <  2:2122749/93‑1 (MQ=255)

                                    tACCGAGTCAAAGCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACCGTCACTTCGGTGGTCACGCCGAGCATACCTTc                                                     <  2:2008637/93‑1 (MQ=255)

                                     aCCGAGTCAAAGCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACCGTCACTTCGGTGGTCACGCCGAGCATACCTTcc                                                    <  2:3321591/93‑1 (MQ=255)

                                     aCCGAGTCAAAGCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACCGTCACTTCGGTGGTCACGCCGAGCATACCTTcc                                                    >  2:3633087/1‑93 (MQ=255)

                                       cGAGTCAAAGCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACCGTCACTTCGGTGGTCACGCCGAGCATACCTTCCGa                                                  <  1:2737419/93‑1 (MQ=255)

                                        gAGTCAAAGCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACCGTCACTTCGGTGGTCACGCCGAGCATACCTTCCGac                                                 >  2:1403504/1‑92 (MQ=255)

                                         aGTCAAAGCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACCGTCACTTCGGTGGTCACGCCGAGCATACCTTCCGATc                                                <  1:3853959/93‑1 (MQ=255)

                                         aGTCAAAGCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACCGTCACTTCGGTGGTCACGCCGAGCATACCTTCCGATc                                                <  1:592097/93‑1 (MQ=255)

                                          gTCAAAGCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACCGTCACTTCGGTGGTCACGCCGAGCATACCTTCCGATcg                                               >  1:3155588/1‑92 (MQ=255)

                                            cAAAGCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACCGTCACTTCGGTGGTCACGCCGAGCATACCTTCCGATCCgg                                             >  1:1591475/1‑93 (MQ=255)

                                            cAAAGCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACCGTCACTTCGGTGGTCACGCCGAGCATACCTTCCGATCCgg                                             <  2:21716/93‑1 (MQ=255)

                                              aaGCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACCGTCACTTCGGTGGTCACGCCGAGCATACCTTCCGATCCGGTg                                           >  1:2030584/1‑93 (MQ=255)

                                               aGCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACCGTCACTTCGGCGGTCACGCCGAGCATACCTTCCGATGCGGTGa                                          >  1:4372718/1‑93 (MQ=255)

                                               aGCTGGCAACCGGAGCCCTGGCCACGGGCGGCTCCGGCAGCAGCTTAACCGTCACTTCGGTGGTCACGCCGAGCATACCTTCCGATCCGGTGa                                          <  2:1933521/93‑1 (MQ=255)

                                                gCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACCGTCACTTCGGTGGTCACGCCGAGCATACCTTCCGATCCGGTGaa                                         <  1:1455872/93‑1 (MQ=255)

                                                  tGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACCGTCACTTCGGTGGTCACGCCGAGCATACCTTCCGATCCGGTGAACa                                       >  1:2504397/1‑93 (MQ=255)

                                                  tGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACCGTCACTTCGGTGGTCACGCCGAGCATACCTTCCGATCCGGTGAACa                                       <  1:1976570/93‑1 (MQ=255)

                                                  tGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACCGTCACTTCGGTGGTCACGCCGAGCATACCTTCCGATCCGGTGAACa                                       >  2:4041802/1‑93 (MQ=255)

                                                  tGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACCGTCACTTCGGTGGTCACGCCGAGCATACCTTCCGATCCGGTGAACa                                       >  2:2791257/1‑93 (MQ=255)

                                                  tGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACCGTCACTTCGGTGGTCACGCCGAGCATACCTTCCGATCCGGTGAACa                                       >  1:61818/1‑93 (MQ=255)

                                                  tGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACCGTCACTTCGGTGGTCACGCCGAGCATACCTTCCGATCCGGTGAACa                                       >  1:960738/1‑93 (MQ=255)

                                                      tAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACCGTCACTTCGGTGGTCACGCCGAGCATACCTTCCGATCCGGTGAACAgcgc                                   >  2:3199731/1‑93 (MQ=255)

                                                       aaCAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACCGTCACTTCGGTGGTCACGCCGAGCATACCTTCCGATCCGGTGAACAGCGcc                                  >  1:2328110/1‑93 (MQ=255)

                                                        aCAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACCGTCACTTCGGTGGTCACGCCGAGCATACCTTCCGATCCGGTGAACAGCGCca                                 >  1:2814585/1‑93 (MQ=255)

                                                        aCAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACCGTCACTTCGGTGGTCACGCCGAGCATACCTTCCGATCCGGTGAACAGCGCca                                 <  2:3405528/93‑1 (MQ=255)

                                                            gaCCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACCGTCACTTCGGTGGTCACGCCGAGCAGACCTTCCGATCCGGTGAACAGCGCcagcag                             <  2:2256452/92‑1 (MQ=255)

                                                            aaCCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACCGTCACTTCGGTGGTCACGCCGAGCATACCTTCCGATCCGGTGAACAGCGCcagcag                             >  1:1672117/1‑93 (MQ=255)

                                                            aaCCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACCGTCACTTCGGTGGTCACGCCGAGCATACCTTCCGATCCGGTGAACAGCGCcagcag                             <  1:2248288/93‑1 (MQ=255)

                                                            aaCCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACCGTCACTTCGGTGGTCACGCCGAGCATACCTTCCGATCCGGTGAACAGCGCcagcag                             <  2:2256451/93‑1 (MQ=255)

                                                               ccGCGCCACGGGCGGCTTCGGCAGCAGCTTTACCGTCACTTCGGTGGTCACGCCGAGCATACCTTCCGATCCGGTGAACAGCGCCAGCAGGTc                          >  1:3245433/1‑93 (MQ=255)

                                                               ccGCGCCACGGGCGGCTTCGGCAGCAGCTTTACCGTCACTTCGGTGGTCACGCCGAGCATACCTTCCGATCCGGTGAACAGCGCCAGCAGGTc                          >  2:1726938/1‑93 (MQ=255)

                                                                 gcgcCACGGGCGGCTTCGGCAGCAGCTTTACCGTCACTTCGGTGGTCACGCCGAGCATACCTTCCGATCCGGTGAACAGCGCCAGCAGGTCaa                        <  1:1079740/93‑1 (MQ=255)

                                                                 gcgcCACGGGCGGCTTCGGCAGCAGCTTTACCGTCACTTCGGTGGTCACGCCGAGCATACCTTCCGATCCGGTGAACAGCGCCAGCAGGTCaa                        <  1:1122117/93‑1 (MQ=255)

                                                                   gcCACGGGCGGCTTCGGCAGCAGCTTTACCGTCACTTCGGTGGTCACGCCGAGCATACCTTCCGATCCGGTGAACAGCGCCAGCAGGTCaaaa                      <  2:3024173/93‑1 (MQ=255)

                                                                     cACGGGCGGCTTCGGCAGCAGCTTTACCGTCACTTCGGTGGTCACGCCGAGCATACCTTCCGATCCGGTGAACAGCGCCAGCAGGTCAAAAcc                    >  1:1372226/1‑93 (MQ=255)

                                                                     cACGGGCGGCTTCGGCAGCAGCTTTACCGTCACTTCGGTGGTCACGCCGAGCATACCTTCCGATCCGGTGAACAGAGCCCGCAGGTCACAAca                    >  2:1502798/1‑92 (MQ=255)

                                                                      aCGGGCGGCTTCGGCAGCAGCTTTACCGTCACTTCGGTGGTCACGCCGAGCATACCTTCCGATCCGGTGAACAGCGCCAGCAGGTCAAAACCa                   <  2:2099717/93‑1 (MQ=255)

                                                                         ggcggcTTCGGCAGCAGCTTTACCGTCACTTCGGTGGTCACGCCGAGCATACCTTCCGATCCGGTGAACAGCGCCAGCAGGTCAAAACCAGGt                <  1:4607306/93‑1 (MQ=255)

                                                                         gccggcTTCGGCAGCAGCTTTACCGTCACTTCGGTGGTCACGCCGAGCATACCTTCCGATCCGGTGAACAGCGCCAGCAGGTCAAAACCAGGt                <  1:3645360/91‑1 (MQ=255)

                                                                            ggcTTCGGCAGCAGCTTTACCGTCACTTCGGTGGTCACGCCGAGCATACCTTCCGATCCGGTGAACAGCGCCAGCAGGTCAAAACCAGGTGaa             <  2:2602655/93‑1 (MQ=255)

                                                                            ggcTTCGGCAGCAGCTTTACCGTCACTTCGGTGGTCACGCCGAGCATACCTTCCGATCCGGTGAACAGCGCCAGCAGGTCAAAACCAGGTGaa             <  2:3534584/93‑1 (MQ=255)

                                                                            ggcTTCGGCAGCAGCTTTACCGTCACTTCGGTGGTCACGCCGAGCATACCTTCCGATCCGGTGAACAGCGCCAGCAGGTCAAAACCAGGTGaa             >  1:4239534/1‑93 (MQ=255)

                                                                              cTTCGGCGCTAGCTTTACCGTCACTTCGGTGGTTGCGCCGAGCATACCTTCCGATCCGGTGAACAGCGCCAGCAGGTCAAAACCAGGTGAATc           <  1:980335/93‑1 (MQ=255)

                                                                              cTTCGGCAGCAGCTTTACCGTCACTTCGGTGGTCACGCCGAGCATACCTTCCGATCCGGTGAACAGCGCCAGCAGGTCAAAACCAGGTGAATc           <  1:719464/93‑1 (MQ=255)

                                                                               ttCGGCAGCAGCTTTACCGTCACTTCGGTGGTCACGCCGAGCATCCCTTCCGATCCGGTGAACAGCGCCAGCAGGTCAAAACCAGGTGAATcc          <  2:4268493/93‑1 (MQ=255)

                                                                               ttCGGCAGCAGCGTTACCGTCACTTCGGTGGTCACGCCGAGCATGCCTTCCGATCCGGTGAACAGCGCCAGCAGGTCAAAACCAGGTGAATcc          <  2:3344181/93‑1 (MQ=255)

                                                                                tCGGCAGCAGCTTTACCGTCACTTCGGTGGTCACGCCGAGCATACCTTCCGATCCGGTGAACAGCGCCAGCAGTTCAAAACCAGCTGAATCCa         <  2:4582686/93‑1 (MQ=255)

                                                                                tCGGCAGCAGCTTTACCGTCACTTCGGTGGTCACGCCGAGCATACCTTCCGATCCGGTGAACAGCGCCAGCAGGTCAAAACCAGGTGAATCCa         <  2:2479154/93‑1 (MQ=255)

                                                                                tCGGCAGCAGCTTTACCGTCACTTCGGTGGTCACGCCGAGCATACCTTCCGATCCGGTGAACAGCGCCAGCAGGTCAAAACCAGGTGAATCCa         <  1:2117720/93‑1 (MQ=255)

                                                                                 cGGCAGCAGCTTTACCGTCACTTCGGTGGTCACGCCGAGCATACCTTCCGATCCGGTGAACAGCGCCAGCAGGTCAAAACCAGGTGAATCCAg        <  2:4647644/93‑1 (MQ=255)

                                                                                  ggCAGCAGCTTTACCGTCACTTCGGTGGTCACGCCGAGCATACCTTCCGATCCGGTGAACAGCGCCAGCAGGTCAAAACCAGGTGAATCCAgc       <  2:3148453/93‑1 (MQ=255)

                                                                                     agcagcTTTACCGTCACTTCGGTGGTCACGCCGAGCATACCTTCCGATCCGGTGAACAGCGCCAGCAGGTCAAAACCAGGTGAATCCAGcgcg    >  1:351376/1‑93 (MQ=255)

                                                                                      gcagCTTTACCGTCACTTCGGTGGTCACGCCGAGCATACCTTCCGATCCGGTGAACAGCGCCAGCAGGTCAAAACCAGGTGAATCCAGCGCGt   >  2:4390980/1‑93 (MQ=255)

                                                                                      gcagCTTTACCGTCACTTCGGTGGTCACGCCGAGCATACCTTCCGATCCGGTGAACAGCGCCAGCAGGTCAAAACCAGGCGAATCCAGCGCGt   >  1:3509174/1‑93 (MQ=255)

GGCGATGATGTCACCAACCGCAAGTCCGGCTTTTTCTACCGAGTCAAAGCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGTTTTACCGTCACTTCGGTGGTCACGCCGAGCATACCTTCCGATCCGGTGAACAGCGCCAGCAGGTCAAAACCAGGTGAATCCAGCGCGTC  >  NC_000913/3125269‑3125448

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`