BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,162,333	A→G	Y553H (TAT→CAT)	parC ←	DNA topoisomerase IV subunit A

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,162,333	0	A	G	100.0%	468.5 / NA	148	Y553H (TAT→CAT)	parC	DNA topoisomerase IV subunit A
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (32/116); total (32/116)

Only 100 of 184 total aligned reads displayed.

AACGGTCGCCCCAGGCGGCAACGTTAATTTGCCGGTGAGCGGCTCGCCCTGACCACGCGCCGACGGCAGCGTAATCGGGTCAATGGCATAGCTACGACCGGTGGAATCAACAAACACTACCGGTTGGTTGCTCTTACCTTTCACCGCCGCTTTGAAGCTATCACCCGCTTTATAATTCA  >  NC_000913/3162244‑3162422

aaCGGTCGCCCCAGGCGGCAACGTTAATTTGCCGGTGAGCGGCTCGCCCTGACCACGCGCCGACGGCAGCGTAATCGGGTCAATGGCATGGCt                                                                                        <  1:1202075/93‑1 (MQ=255)

aaCGGTCGCCCCAGGCGGCAACGTTAATTTGCCGGTGAGCGGCTCGCCCTGACCACGCGCCGACGGCAGCGGAATCGGGTCAATGGCATGGCt                                                                                        >  2:1041946/1‑93 (MQ=255)

 aCGGTCGCCCCAGGCGGCAACGTTAATTTGCCGGTGAGCGGCTCGCCCTGACCCCGCGCCGACGGCAGCGGAATCGGGGCAATGGGATGGCTa                                                                                       >  2:3205424/1‑93 (MQ=255)

 aCGGTCGCCCCAGGCGGCAACGTTAATTTGCCGGTGAGCGGCTCGCCCTGACCACGCGCCGACGGCAGCGTAATCGGGTCAATGGCATGGCTa                                                                                       <  1:4111909/93‑1 (MQ=255)

 aCGGTCGCCCCAGGCGGCAACGTTAATTTGCCGGTGAGCGGCTCGCCCCGGCCCCGCTCCGACGGCGGCGCCATCGGGGCAATGGCATGGCTa                                                                                       >  2:2229609/1‑93 (MQ=255)

  cGGTCGCCCCAGGCGGCAACGTTAATTTGCCGGTGAGCGGCTCGCCCTGACCACGCGCCGACGGCAGCGTAATCGGGTCAATGGCATGGCTAc                                                                                      <  1:1389043/93‑1 (MQ=255)

    gTCGCCCCAGGCGGCAACGTTAATTTGCCGGTGAGCGGCTCGCCCTGACCACGCGCCGACGGCAGCGTAATCGGGTCAATGGCATGGCTACGa                                                                                    <  1:2939959/93‑1 (MQ=255)

    gTCGCCCCAGGCGGCAACGTTAATTTGCCGGTGAGCGGCTCGCCCTGACCACGCGCCGACGGCAGCGTAATCGGGTCAATGGCATGGCTACGa                                                                                    <  1:2783549/93‑1 (MQ=255)

     tCGCCCCAGGCGGCAACGTTAATTTGCCGGTGAGCGGCTCGCCCTGACCACGCGCCGACGGCAGCGTAATCGGGTCAATGGCATGGCTACGAc                                                                                   <  1:4458814/93‑1 (MQ=255)

      cGCCCCAGGCGGCAACGTTAATTTGCCGGTGAGCGGCTCGCCCTGACCACGCGACGACGGCAGCGTAATCGGGGCAATGGCATGGCTACGAcc                                                                                  >  1:4430140/1‑93 (MQ=255)

       gCCCCAGGCGGCAACGTTAATTTGCCGGTGAGCGGCTCCCCCTGACCACGCGCCGACGGCAGCCGAATCCGGTCCATTGCATGGCTACGGCCg                                                                                 >  2:205005/1‑93 (MQ=255)

        ccccAGGCGGCAACGTTAATTTGCCGGTGAGCGGCTCGCCCTGACCACGCGCCGACGGCAGCGTAATCGGGTCAATGGCATGGCTACGACCgg                                                                                <  1:2382304/93‑1 (MQ=255)

        ccccAGGCGGCAACGTTAATTTGCCGGTGAGCGGCTCGCCCTGACCACGCGACGACGGCCGCGTCATCGGGGCAATGGCATGGCTACGAcacg                                                                                >  1:28633/1‑90 (MQ=255)

         cccATGCGCCAGCGTTAATTTGCCGGTGAGCGGCTCGCCCTGACCACGCGCCGACGGCAGCGTAATCGGGTCAATGGCATGGCTACGACCGGt                                                                               <  1:2607591/93‑1 (MQ=255)

         cccAGGCGGCAACGTTAATTTGCCGGTGAGCGGCTCGCCCTGACCACGCGCCGACGGCAGCGTAATCGGGGCAATGGCATGGCTACGACCggg                                                                               >  1:1039838/1‑92 (MQ=255)

           cAGGCGGCAACGTTAATTTGCCGGTGAGCGGCTCGCCCCGACCACGCGCCGACGGCAGCGTAATAGGGGCAATGGCACGGCTACGCCCGGTgg                                                                             >  2:4363119/1‑93 (MQ=255)

              gcggcAACGTTAATTTGCCGGTGAGCGGCTCGCCCTGACCACGCGCCGACGGCAGCGTAATCGGGTCAATGGCATGGCTACGACCGGTGGAAt                                                                          <  1:968601/93‑1 (MQ=255)

               cggcAACGTTAATTTGCCGGTGAGCGGCTCGCCCTGACCACGCGCCGACGGCAGCGTAATCGGGTCAATGGCATGGCTACGACCGGTGGAATc                                                                         <  1:1382938/93‑1 (MQ=255)

                ggcAACGTTAATTTGCCGGTGAGCGGCTCGCCCTGACCACGCGCCGACCGCAGCGTAATCGGGGCAATGGCATGGCTACGCCCCGGGGGATca                                                                        >  1:4271012/1‑93 (MQ=255)

                 gcAACGTTAATTTGCCGGTGAGCGGCTCGCCCTGACCACGCGCCGACGGCAGCGTAATCGGGTCAATGGCATGGCTACGACCGGGGGCATcaa                                                                       >  1:1668125/1‑93 (MQ=255)

                 gcAACGTTAATTTGCCGGTGAGCGGCTCCCCCTGACCACGCGCCGACGGCAGCGGAATCGGGGCAATGGCATGGCTACGACCGGGGGGATcca                                                                       >  1:1509781/1‑91 (MQ=255)

                  cAACGTTAATTTGCCGGTGAGCGGCTCGCCCTGACCGCGCGCCGACGGCAGCGTAATCGGGTCAATGGCATGGCTACGACCGGTGGAATcaac                                                                      <  1:2315927/93‑1 (MQ=255)

                  cAACGTTAATTTGCCGGTGAGCGGCTCGCCCTGACCACGCGCCGACGGCATCGTAATCGGGTCAATGGCATGGCTACGACCGGTGGAATcaac                                                                      <  1:2512530/93‑1 (MQ=255)

                  cAACGTTAATTTGCCGGTGAGCGGCTCGCCCTGACCACGCGCCGACGGCAGCGTAATCGGGGCAATGGCATGGCTACGACCGGTGGAATcaac                                                                      >  2:4045279/1‑93 (MQ=255)

                  cAACGTTAATTTGCCGGTGAGCGGCTCGCCCTGACCACGCGCCGACGGCAGCGGAATCGGGTCAATGGCATGGTTACGACCGGTGGTATcaaa                                                                      >  2:2661940/1‑92 (MQ=255)

                   aaCGTTAATTTGCCGGTGAGCGGCTCGCCCTGACCACGCGCCGACGGCAGCGTAATCGGGTCAATGGCATGGCTACGACCGGTGGAATcaaca                                                                     <  2:3535189/93‑1 (MQ=255)

                   aaCGTTAATTTGCCGGTGAGCGGCTCGCCCTGACCACGCGCCGACGGCAGCGTAATCGGGGCAATGGCATGGCTACGACCCGTGGGATcaaca                                                                     >  1:48697/1‑93 (MQ=255)

                    aCGTTAATTTGCCGGTGAGCGGCTCGCCCTGACCACGCGCCGACGGCAGCGTAATCGGGTCAATGGCATGGCTACGACCGGGGGGATcaacaa                                                                    >  1:4363133/1‑93 (MQ=255)

                    aCGTTAATTTGCCGGTGAGCGGCTCGCCCTGACCACGCGCCGACGGCAGCGTAATCGGGTCAATGGCATGGCTACCACCGGGGGAATcaacaa                                                                    >  2:2817263/1‑93 (MQ=255)

                       ttAATTTGCCGGTGAGCGGCTCGCCCTGACCACGCGCCGACGGCAGCGTAATCGGGTCAATGGCATGGCTACGACCGGTGGAATCaacaaaca                                                                 <  1:3210969/93‑1 (MQ=255)

                        tAATTTGCCGGTGAGCGGCTCGCCCTGACCACGCGCCGACGGCAGCGTAATCGGGTCAATGGCATGGCTACGACCGGTGGAATCAACAAacac                                                                <  1:2623222/93‑1 (MQ=255)

                        tAATTTGCCGGTGAGCGGCTCGCCCTGACCACGCGCCGACGGCAGCGTAATCGGGTCAATGGCATGGCTACGACCGGTGGAATCAACAAacac                                                                <  2:1282196/93‑1 (MQ=255)

                        tAATTTGCCGGTGAGCGGCTCGCCCTGACCACGCGCCGACGGCAGCGTAATCGGGTCAATGGCATGGCTACGACCGGTGGAATCAACAAacac                                                                <  1:757326/93‑1 (MQ=255)

                          atgtGCCGGTGAGCGGCTCGCCCTGACCACGCGCCGACGGCAGCGTAATCGGGTCAATGGCATGGCTACGACCGGTGGAATCAACAAACACTa                                                              <  1:1835932/90‑1 (MQ=255)

                           tttGCCGGTGAGCGGCTCGCCCTGACCACGCGCCGACGGCAGCGTAATCGGGTCAATGGCATGGCTACGACCGGTGGAATCAACAAACACTAc                                                             <  2:4646438/93‑1 (MQ=255)

                           tttGCCGGTGAGCGGCTCGCCCTGACCACGCGCCGACGGCAGCGTAATCGGGTCAATGGCATGGCTACGACCGGTGGAATCAACAAACACTAc                                                             <  1:1432840/93‑1 (MQ=255)

                           tttGCCGGTGAGCGGCTCGCCCTGACCACGCGCCGACGGCAGCGTAATCGGGTCAATGGCATGGCTACGACCGGTGGAATCAACAAACACTAc                                                             <  2:1631244/93‑1 (MQ=255)

                           tttGCCGGTGAGCGGCTCGCCCTGACCACGCGCCGACGGCAGCGTAAACGGGGCAATGGCATGGCTAAGAGCCGGGGGATCAACAAACACTAc                                                             >  2:2519458/1‑93 (MQ=255)

                             tGCCGGTGAGCGGCTCGCCCTGACCACGCGCCGACGGCAGCGTAATCGGGTCAATGGCATGGCTACGACCGGTGGAATCAACAAACACTACCg                                                           <  2:2374570/93‑1 (MQ=255)

                               ccGGTGAGCTGCTCGCCCTGACCACGCGCCGACGGCAGCGTAATCGGGTCAATGGCATGGCTACGACCGGTGGAATCAACAAACACTACCggt                                                         <  2:4076698/93‑1 (MQ=255)

                               ccGGTGAGCGGCTCGCCCTGACCACGCGCCGACGTCAGCGTAATCGGGTCAATGGCATGGCTACGACCGGTGGAATCAACAAACACTACCggt                                                         <  1:1447447/93‑1 (MQ=255)

                                cGGTGAGCGGCTCGCCCTGACCACGCGCCGACGGCAGCGTAATCGGGTCAATGGCATGGCTACGACCGGTGGAATCAACAAACACTACCggtt                                                        <  1:1914997/93‑1 (MQ=255)

                                  gTGAGCGGCTCGCCCTGACCACGCGCCGACGGCAGCGTAATCGGGGCAATGGCATGGCTACGACCGGTGGGATCAACAAAAACTACCcgtttg                                                      >  2:1700921/1‑91 (MQ=255)

                                   tGAGCGGCTCGCCCTGACCACGCGCCGACGGCAGCGTAATCGGGTCAATGGCATGGCTACGACCGGTGGAATCAACAAACACTACCggttggt                                                     <  1:1099679/93‑1 (MQ=255)

                                   tGAGCGGCTCGCCCTGACCACGCGCCGACGGCAGCGTAATCGGGTCAATGGCATGGCTACGACCGGTGGAATCAACAAACACTACCggttggt                                                     <  2:1558313/93‑1 (MQ=255)

                                    gAGCGGCTCGCCCTGACCACGCGCCGACGGCAGCGTAATCGGGTCAATGGCATGGCTACGACCGGTGGAATCAACAAACACTACCggttggtt                                                    >  1:3737029/1‑93 (MQ=255)

                                      gCGGCTCGCCCTGACCACGCGCCGACGGCAGCGTAATCGGGTCAATGGCATGGCTACGACCGGTGGAATCAACAAACACTACCGGTTGGTTGc                                                  <  1:4534034/93‑1 (MQ=255)

                                      gCGGCTCGCCCTGACCACGCGCCGACGGCAGCGTAATCGGGTCAATGGCATGGCTACGACCGGTGGAATCAACAAACACTACCGGTTGGTTGc                                                  <  1:4175421/93‑1 (MQ=255)

                                        ggCTCGCCCTGACCACGCGCCGACGGCAGCGTAATCGGGTCAATGGCATGGCTACGACCGGTGGAATCAACAAACACTACCGGTTGGTTGctc                                                >  2:3297303/1‑93 (MQ=255)

                                        ggCTCGCCCGGACCACGCGCCGACGGCAGCGTAATCGGGTCAATGGCATGGCTACGACCGGTGGAATCAACAAACACTACCGGTTGGTTGctc                                                <  2:2598156/93‑1 (MQ=255)

                                         gCTCGCCCTGACCACGCGCCGACGGCAGCGTAATCGGGTCAATGGCATGGCTACGACCGGTGGAATCAACAAACACTACCGGTTGGTTGctct                                               <  2:3935331/93‑1 (MQ=255)

                                             gCCCTGACCACGCGCCGACGGCAGCGTAATCGGGTCAATGGCATGGCTACGACCGGTGGAATCAACAAACACTACCGGTTGGTTGCTCTTAcc                                           <  2:3895036/93‑1 (MQ=255)

                                             gCCCTGACCACGCGCCGACGGCAGCGTAATCGGGTCAATGGCATGGCTACGACCGGTGGAATCAACAAACACTACCGGTTGGTTGCTCTTAcc                                           >  1:1275022/1‑93 (MQ=255)

                                               ccTGACCACGCGCCGACGGCAGCGTAATCGGGTCAATGGCATGGCTACGACCGGTGGAATCAACAAACACTACCGGTTGGTTGCTCTTACCtt                                         <  2:1919042/93‑1 (MQ=255)

                                               ccTGACCACGCGCCGACGGCAGCGGAATCGGGTCAATGGCATGGCTACGACCGGTGAAATCAACAAACACTACCGGTTGGTTGCTCTTACCtt                                         <  1:2449894/93‑1 (MQ=255)

                                                cTGACCACGCGCCGACGGCAGCGTAATCGGGTCAATGGCATGGCTACGACCGGTGGAATCAACAAACACTACCGGTTGGTTGCTCTTACCttt                                        >  1:4578225/1‑93 (MQ=255)

                                                cTGACCACGCGCCGACGGCAGCGTAATCGGGTCAATGGCATGGCTACGACCGGTGGAATCAACAAACACTACCGGTTGGTTGCTCTTACCttt                                        >  2:4231370/1‑93 (MQ=255)

                                                cTGACCACGCGCCGACGGCAGCGTAATCGGGTCAATGGCATGGCTACGACCGGTGGAATCAACAAACACTACCGGTTGGTTGCTCTTACCttt                                        <  2:3065140/93‑1 (MQ=255)

                                                  gACCACGCGCCGACGGCAGCGTAATCGGGTCAATGGCATGGCTACGACCGGTGGAATCAACAAACACTACCGGTTGGTTGCTCTTACCTTTCa                                      >  1:2296755/1‑93 (MQ=255)

                                                  gACCACGCGCCGACGGCAGCGTAATCGGGTCAATGGCATGGCTACGACCGGTGGAATCAACAAACACTACCGGTTGGTTGCTCTTACCTTTCa                                      <  2:4201187/93‑1 (MQ=255)

                                                   aCCACGCGCCGACGGCAGCGTAATCGGGTCAATGGCATGGCTACGACCGGTGGAATCAACAAACACTACCGGTTGGTTGCTCTTACCTTTCAc                                     <  2:3868612/93‑1 (MQ=255)

                                                    ccACGCGCCGACGGCAGCGTAATCGGGTCAATGGCATGGCTACGACCGGTGGAATCAACAAACACTACCGGTTGGTTGCTCTTACCTTTCAcc                                    <  2:3708448/93‑1 (MQ=255)

                                                      aCGCGCCGCCGGCAGCGTAATCGGGTCAATGGCATGGCTACGACCGGTGGAATCAACAAACACTACCGGTTGGTTGCTCTTACCTTTCAccgc                                  <  2:123558/93‑1 (MQ=255)

                                                         cgcCGACGGCAGCGTAATCGGGTCAATGGCATGGCTACGACCGGTGGAATCAACAAACACTACCGGTTGGTTGCTCTTACCTTTCACcgccgc                               <  2:2005973/93‑1 (MQ=255)

                                                          gcCGACGGCAGCGTAATCGGGTCAATGGCATGGCTACGACCGGTGGAATCAACAAACACTACCGGTTGGTTGCTCTTACCTTTCACCGCCGCt                              <  1:1721045/93‑1 (MQ=255)

                                                           ccGACGGCAGCGTAATCGGGTCAATGGCATGGCTACGACCGGTGGAATCAACAAACACTACCGGTTGGTTGCTCTTACCTTTCACCGCCGCtt                             <  1:1999794/93‑1 (MQ=255)

                                                           ccGACGGCAGCGTAATCGGGTCAATGGCATGGCTACGACCGGTGGAATCAACAAACACTACCGGTTGGTTGCTCTTACCTTTCACCGCCGCtt                             <  1:3332424/93‑1 (MQ=255)

                                                           ccGACGGCAGCGTAATCGGGTCAATGGCATGGCTACGACCGGTGGAATCAACAAACACTACCGGTTGGTTGCTCTTACCTTTCACCGCCGCtt                             <  1:4205308/93‑1 (MQ=255)

                                                            cGACGGCAGCGTAATCGGGTCAATGGCATGGCTACGACCGGTGGAATCAACAAACACTACCGGTTGGTTGCTCTTACCTTTCACCGCCGCttt                            <  1:778018/93‑1 (MQ=255)

                                                            cGACGGCAGCGTAATCGGGTCAATGGCATGGCTACGACCGGTGGAATCAACAAACACTACCGGTTGGTTGCTCTTACCTTTCACCGCCGCttt                            <  2:4505471/93‑1 (MQ=255)

                                                             gACGGCAGCGTAATCGGGTCAATGGCATGGCTACGACCGGTGGAATCAACAAACACTACCGGTTGGTTGCTCTTACCTTTCACCGCCGCTTTg                           >  2:1075572/1‑93 (MQ=255)

                                                             gACGGCAGCGTAATCGGGTCAATGGCATGGCTACGACCGGTGGAATCAACAAACACTACCGGTTGGTTGCTCTTACCTTTCACCGCCGCTTTg                           <  2:2423430/93‑1 (MQ=255)

                                                              aCGGCAGCGTAATCGGGTCAATGGCATGGCTACGACCGGTGGAATCAACAAACACTACCGGTTGGTTGCTCTTACCTTTCACCGCCGCTTTGa                          <  2:2493311/93‑1 (MQ=255)

                                                              aCGGCAGCGTAATCGGGTCAATGGCATGGCTACGACCGGTGGAATCAACAAACACTACCGGTTGGTTGCTCTTACCTTTCACCGCCGCTTTGa                          <  2:575401/93‑1 (MQ=255)

                                                               cGGCAGCGTAATCGGGTCAATGGCATGGCTACGACCGGTGGAATCAACAAACACTACCGGTTGGTTGCTCTTACCTTTCACCGCCGCTTTGaa                         <  2:2306732/93‑1 (MQ=255)

                                                               cGGCAGCGTAATCGGGTCAATGGCATGGCTACGACCGGTGGAATCAACAAACACTACCGGTTGGTTGCTCTTACCTTTCACCGCCGCTTTGaa                         <  2:1932167/93‑1 (MQ=255)

                                                                ggCAGCGTAATCGGGTCAATGGCATGGCTACGACCGGTGGAATCAACAAACACTACCGGTTGGTTGCTCTTACCTTTCACCGCCGCTTTGAAg                        >  1:2665342/1‑93 (MQ=255)

                                                                 gCAGCGTAATCGGGTCAATGGCATGGCTACGACCGGTGGAATCAACAAACACTACCGGTTGGTTGCTCTTACCTTTCACCGCCGCTTTGAAGc                       <  1:3498057/93‑1 (MQ=255)

                                                                 gCAGCGTAATCGGGTCAATGGCATGGCTACGACCGGTGGAATCAACAAACACTACCGGTTGGTTGCTCTTACCTTTCACCGCCGCTTTGAAGc                       <  2:189376/93‑1 (MQ=255)

                                                                 gCAGCGTAATCGGGTCAATGGCATGGCTACGACCGGTGGAATCAACAAACACTACCGGTTGGTTGCTCTTACCTTTCACCGCCGCTTTGAAGc                       <  2:3556649/93‑1 (MQ=255)

                                                                   aGCGTAATCGGGTCAATGGCATGGCTACGACCGGTGGAATCAACAAACACTACCGGTTGGTTGCTCTTACCTTTCACCGCCGCTTTGAAGCTa                     >  2:1165703/1‑93 (MQ=255)

                                                                   aGCGTAATCGGGTCAAGGGCATGGCTACGACCGGTGGAATCAACAAACACTACCGGTTGGTTGCTCTTACCTTTCACCGCCGCTTTGAAGCTa                     <  1:986913/93‑1 (MQ=255)

                                                                    gCGTAATCGGGTCAATGGCATGGCTACGACCGGTGGAATCAACAAACACTACCGGTTGGTTGCTCTTACCTTTCACCGCCGCTTTGAAGCTAt                    <  2:1774321/93‑1 (MQ=255)

                                                                     cGTAATCGGGTCAATGGCATGGCTACGACCGGTGGAATCAACAAACACTACCGGTTGGTTGCTCTTACCTTTCACCGCCGCTTTGAAGCTATc                   <  2:884458/93‑1 (MQ=255)

                                                                     cGTAATCGGGTCAATGGCATGGCTACGACCGGTGGAATCAACAAACACTACCGGTTGGTTGCTCTTACCTTTCACCGCCGCTTTGAAGCTATc                   <  1:1086858/93‑1 (MQ=255)

                                                                       tAATCGGGTCAATGGCATGGCTACGACCGGTGGAATCAACAAACACTACCGGTTGGTTGCTCTTACCTTTCACCGCCGCTTTGAAGCTATCAc                 >  1:3844850/1‑93 (MQ=255)

                                                                        aaTCGGGTCAATGGCATGGCTACGATCGGTGGAATCAACAAACACTACCGGTTGGTTGCTCTTACCTTTCACCGCCGCTTTGAAGCTATCAcc                <  1:4527777/93‑1 (MQ=255)

                                                                             ggTCAATGGCATGGCTACGACCGGTGGAATCAACAAACACTACCGGTTGGTTGCTCTTACCTTTCACCGCCGCTTTGAAGCTATCACCCGCtt           <  2:3818028/93‑1 (MQ=255)

                                                                              gTCAATGTCATGGCTACGACCGGTGGAATCAACAAACACTACCGGTTGGTTGCTCTTACCTTTCACCGCCGCTTTGAAGCTATCACCCGCttt          <  2:2752490/93‑1 (MQ=255)

                                                                              gTCAATGGCATGGCTACGACCGGTGGAATCAACAAACACTACCGGTTGGTTGCTCTTACCTTTCACCGCCGCTTTGAAGCTATCACCCGCttt          <  1:893867/93‑1 (MQ=255)

                                                                              gTCAATGGCATGGCTACGACCGGTGGAATCAACAAACACTACCGGTTGGTTGCTCTTACCTTTCACCGCCGCTTTGAAGCTATCACCCGCttt          <  1:3592445/93‑1 (MQ=255)

                                                                               tCAATGGCATGGCTACGACCGGTGGAATCAACAAACACTACCGGTTGGTTGCTCTTACCTTTCACCGCCGCTTTGAAGCTATCACCCGCTTta         <  1:4601733/93‑1 (MQ=255)

                                                                               tCAATGGCATGGCTACGACCGGTGGAATCAACAAACACTACCGGTTGGTTGCTCTTACCTTTCACCGCCGCTTTGAAGCTATCACCCGCTTta         <  1:3354770/93‑1 (MQ=255)

                                                                               tCAATGGCATGGCTACGACCGGTGGAATCAACAAACACTACCGGTTGGTTGCTCTTACCTTTCACCGCCGCTTTGAAGCTATCACCCGCTTta         <  1:2388737/93‑1 (MQ=255)

                                                                                 aaTGGCATGGCTACGACCGGTGGAATCAACAAACACTACCGGTTGGTTGCTCTTACCTTTCACCGCCGCTTTGAAGCTATCACCCGCTTtata       <  2:393398/93‑1 (MQ=255)

                                                                                 aaTGGCATGGCTACGACCGGTGGAATCAACAAACACTACCGGTTGGTTGCTCTTACCTTTCACCGCCGCTTTGAAGCTATCACCCGCTTtata       <  2:2184554/93‑1 (MQ=255)

                                                                                 aaTGGCATGGCTACGACCGGTGGAATCAACAAACACTACCGGTTGGTTGCTCTTACCTTTCACCGCCGCTTTGAAGCTATCACCCGCTTtata       >  1:1365362/1‑93 (MQ=255)

                                                                                    ggCATGGCTACGACCGGTGGAATCAACAAACACTACCGGTTGGTTGCTCTTACCTTTCACCGCCGCTTTGAAGCTATCACCCGCTTTATAAtt    >  1:3984269/1‑93 (MQ=255)

                                                                                      cATGGCTACGACCGGTGGAATCAACAAACACTACCGGTTGGTTGCTCTTACCTTTCACCGCCGCTTTGAAGCTATCACCCGCTTTATAATTCa  >  2:3732912/1‑93 (MQ=255)

                                                                                      cATGGCTACGACCGGTGGAATCAACAAACACTACCGGTTGGTTGCTCTTACCTTTCACCGCCGCTTTGAAGCTATCACCCGCTTTATAATTCa  <  2:4419530/93‑1 (MQ=255)

AACGGTCGCCCCAGGCGGCAACGTTAATTTGCCGGTGAGCGGCTCGCCCTGACCACGCGCCGACGGCAGCGTAATCGGGTCAATGGCATAGCTACGACCGGTGGAATCAACAAACACTACCGGTTGGTTGCTCTTACCTTTCACCGCCGCTTTGAAGCTATCACCCGCTTTATAATTCA  >  NC_000913/3162244‑3162422

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 19 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`