BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,164,677	T→C	K353R (AAG→AGG)	b3020 ←	putative transport periplasmic protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,164,677	0	T	C	100.0%	595.9 / NA	184	K353R (AAG→AGG)	b3020	putative transport periplasmic protein
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (101/83); total (101/83)

Only 100 of 193 total aligned reads displayed.

AGAACAGCTCAAAGCGTAGCGGATGAGAGGCGTCGTATCCCGCCTGTTTCAGCAAGGCTTTTGCCATCGCGACGCGCTCACTCATTGGCTTTTGCAGTTCATCGAACGTCGTCGCGCTAAAGCCTTTTACCTCTGGCGGCGTCAGCGTGGTTGCGGGCGTTCTCAACCCCAGTACCTTT  >  NC_000913/3164588‑3164766

aGAACAGCTCAAAGCGTAGCGGATGAGAGGCGTCGTATCCCGCCTGTTTCAGCAAGGCTTTTGCCATCGCGACGCGCTCACTCATTGGCcttt                                                                                        <  1:4537031/93‑1 (MQ=255)

 gAACAGCTCAAAGCGTAGCGGATGAGAGGCGTCGTATCCCGCCTGTTTCAGCAAGGCTTTTGCCATCGCGACGCGCTCACTCATTGGCCTTTg                                                                                       <  1:3912704/93‑1 (MQ=255)

 gAACAGCTCAAAGCGTAGCGGATGAGAGGCGTCGTATCCCGCCTGTTTCAGCAAGGCTTTTGCCATCGCGACGCGCTCACTCATTGGCCTTTg                                                                                       <  1:2702078/93‑1 (MQ=255)

   aCAGCTCAAAGCGTAGCGGATGAGAGGCGTCGTATCCCGCCTGTTTCAGCAAGGCTTTTGCCATCGCGACGCGCTCACTCATTGGCCTTTGCa                                                                                     >  2:333662/1‑93 (MQ=255)

   aCAGCTCAAAGCGTAGCGGATGAGAGGCGTCGTATCCCGCCTGTTTCAGCAAGGCTTTTGCCATCGCGACGCGCTCACTCATTGGCCTTTGCa                                                                                     <  1:989510/93‑1 (MQ=255)

    cAGCTCAAAGCGTAGCGGATGAGAGGCGTCGTATCCCGCCTGTTTCAGCAAGGCTTTTGCCATCGCGACGCGCTCACTCATTGGCCTTTGCAg                                                                                    <  1:329210/93‑1 (MQ=255)

    cAGCTCAAAGCGTAGCGGATGAGAGGCGTCGTATCCCGCCTGTTTCAGCAAGGCTTTTGCCATCGCGACGCGCTCACTCATTGGCCTTTGCAg                                                                                    <  2:2604740/93‑1 (MQ=255)

    cAGCTCAAAGCGTAGCGGATGAGAGGCGTCGTATCCCGCCTGTTTCAGCAAGGCTTTTGCCATCGCGACGCGCTCACTCATTGGCCTTTGCAg                                                                                    <  1:2163282/93‑1 (MQ=255)

    cAGCTCAAAGCGTAGCGGATGAGAGGCGTCGTATCCCGCCCGTTTCAGCAAGGCTTTTGCCATCGCGACGCGCTCACTCCTTGGCCTCTGCAg                                                                                    >  1:1235808/1‑93 (MQ=255)

    cAGCTCAAAGCGTAGCGGATGAGAGGCGGCGTATCCCGCCTGTTTCAGCAAGGCTTTTGCCATCGCGACGCGCTCACTCATTGGCCTTTGCAg                                                                                    <  1:3757587/93‑1 (MQ=255)

      gCTCAAAGCGTAGCGGATGAGAGGCGTCGTATCCCGCCTGTTTCAGCAAGGCTTTTGCCATCGCGACGCGCTCACTCATTGGCCTTTGCAGtt                                                                                  >  2:2684398/1‑93 (MQ=255)

      gCTCAAAGCGTAGCGGATGAGAGGCGTCGTATCCCGCCTGTTTCAGCAAGGCTTTTGCCATCGCGACGCGCTCACTCATTGGCCTTTGCAGtt                                                                                  >  2:655016/1‑93 (MQ=255)

       cTCAAAGCGTAGCGGATGAGAGGCGTCGTATCCCGCCTGTTTCAGCAAGGCTTTTGCCATCGCGACGCGCTCACTCATTGGCCTTTGCAGTTc                                                                                 <  1:1482165/93‑1 (MQ=255)

        tCAAAGCGTAGCGGATGAGAGGCGTCGTATCCCGCCTGTTTCAGCAAGGCTTTTGCCATCGCGACGCGCTCACTCATTGGCCTTTGCAGTTCa                                                                                <  1:4287633/93‑1 (MQ=255)

        tCAAAGCGTAGCGGATGAGAGGCGTCGTATCCCGCCTGTTTCAGCAAGGCTTTTGCCATCGCGACGCGCTCACTCATTGGCCTTTGCAGTTCa                                                                                <  1:3400407/93‑1 (MQ=255)

        tCAAAGCGTAGCGGATGAGAGGCGTCGTATCCCGCCTGTTTCAGCAAGGCTTTTGCCATCGCGACGCGCTCAATCATTGGCCTTTGCAGTTCa                                                                                >  2:220538/1‑93 (MQ=255)

         cAAAGCGTAGCGGATGAGAGGCGTCGTATCCCGCCTGTTTCAGCAATGCTTTTGCCATCGCGACGCGCTCACTCATTGGCCTTTGCAGTTCAt                                                                               <  2:3782169/93‑1 (MQ=255)

         cAAAGCGTAGCGGATGAGAGGCGTCGTATCCCGCCTGTTTCAGCAAGGCTTTTGCCATCGCGACGCGCTCACTCATTGGCCTTTGCAGTTCAt                                                                               <  2:3981660/93‑1 (MQ=255)

         cAAAGCGTAGCGGATGAGAGGCGTCGTATCCCGCCTGTTTCAGCAAGGCTTTTGCCATCGCGACGCGCTCACTCATTGGCCTTTGCAGTTCAt                                                                               <  2:2885420/93‑1 (MQ=255)

           aaGCGTAGCGGATGAGAGGCGTCGTATCCCGCCTGTTTCAGCAAGGCTTTTGCCATCGCGACGCGCTCACTCATTGGCCTTTGCAGTTCATcc                                                                             >  1:3531182/1‑92 (MQ=255)

             gCGTAGCGGATGAGAGGCGTCGTATCCCGCCTGTTTCAGCAAGGCTTTTGCCATCGCGACGCGCTCACTCATTGGCCTTTGCAGTTCATCGaa                                                                           <  2:3143676/93‑1 (MQ=255)

             gCGTAGCGGATGAGAGGCGTCGTATCCCGCCTGTTTCAGCAAGGCTTTTGCCATCGCGACGCGCTCACTCATTGGCCTTTGCAGTTCATCGaa                                                                           >  2:3467910/1‑93 (MQ=255)

             gCGTAGCGGATGAGAGGCGTCGTATCCCGCCTGTTTCAGCAAGGCTTTTGCCATCGCGACGCGCTCACTCATTGGCCTTTGCAGTTCATCGaa                                                                           <  1:2607140/93‑1 (MQ=255)

              ggTAGCGGATGAGAGGCGTCGTATCCCGCCTGTTTCAGCAAGGCTTTTGCCATCGCGACGCGCTCACTCATTGGCCTTTGCAGTTCATCGAAc                                                                          <  2:1720104/92‑1 (MQ=255)

               gTAGCGGATGAGAGGCGTCGTATCCCGCCTGTTTCAGCAAGGCTTTTGCCATCGCGACGCGCTCACTCATTGGCCTTTGCAGTTCATCGAAcg                                                                         >  2:3382041/1‑93 (MQ=255)

                           agGCGTCGTATCCCGCCTGTTTCAGCAAGGCTTTTGCCATCGCGACGCGCTCACTCATTGGCCTTTGCAGTTCATCGAACGTCGTCGCGCTaa                                                             <  2:2773857/93‑1 (MQ=255)

                            ggCGTCGTATCCCGCCTGTTTCAGCAAGGCTTTTGCCATCGCGACGCGCTCACTCATTGGCCTTTGCAGTTCATCGAACGTCGTCGCGCTaaa                                                            <  1:3154657/93‑1 (MQ=255)

                            ggCGTCGTATCCCGCCTGTTTCAGCAAGGCTTTTGCCATCGCGACGCGCTCACTCATTGGCCTTTGCAGTTCATCGAACGTCGTCGCGCTaaa                                                            <  1:952252/93‑1 (MQ=255)

                            ggCGTCGTATCCCGCCTGTTTCAGCAAGGCTTTTGCCATCGCGACGCGCTCACTCATTGGCCTTTGCAGTTCATCGAACGTCGTCGCGCTaaa                                                            <  1:2283706/93‑1 (MQ=255)

                            ggCGTCGTATCCCGCCTGTTTCAGCAAGGCTTTTGCCATCGCGACGCGCTCACTCATTGGCCTTTGCAGTTCATCGAACGTCGTCGCGCTaaa                                                            <  2:4258161/93‑1 (MQ=255)

                             gCGTCGTATCCCGCCTGTTTCAGCAAGGCTTTTGCCATCGCGACGCGCTCACTCATTGGCCTTTGCAGTTCATCGAACGTCGTCGCGCTAAAg                                                           <  1:3032724/93‑1 (MQ=255)

                             gCGTCGTATCCCGCCTGTTTCAGCAAGGCTTTTGCCATCGCGACGCGCTCACTCATTGGCCTTTGCAGTTCATCGAACGTCGTCGCGCTAAAg                                                           <  1:506961/93‑1 (MQ=255)

                              cgtcgtATCCCGCCTGTTTCAGCAAGGCTTTTGCCATCGCGACGCGCTCACTCATTGGCCTTTGCAGTTCATCGAACGTCGTCGCGCTAAAGc                                                          >  1:4077147/1‑93 (MQ=255)

                              cgtcgtATCCCGCCTGTTTCAGCAAGGCTTTTGCCATCGCGACGCGCTCACTCATTGGCCTTTGCAGTTCATCGAACGTCGTCGCGCTAAAGc                                                          <  2:4104743/93‑1 (MQ=255)

                               gtcgtATCCCGCCTGTTTCAGCAAGGCTTTTGCCATCGCGACGCGCTCACTCATTGGCCTTTGCAGTTCATCGAACGTCGTCGCGCTAAAGcc                                                         <  2:4324777/93‑1 (MQ=255)

                               gtcgtATCCCGCCTGTTTCAGCAAGGCTTTTGCCATCGCGACGCGCTCACTCATTGGCCTTTGCAGTTCATCGAACGTCGTCGCGCTAAAGcc                                                         <  1:4663626/93‑1 (MQ=255)

                                  gtATCCCGCCTGTTTCAGCAAGGCTTTTGCCATCGCGACGCGCTCACTCATTGGCCTTTGCAGTTCATCGAACGTCGTCGCGCTAAAGCCttt                                                      <  1:2953994/93‑1 (MQ=255)

                                  gtATCCCGCCTGTTTCAGCAAGGCTTTTGCCATCGCGACGCGCTCACTCATTGGCCTTTGCAGTTCATCGAACGTCGTCGCGCTAAAGCCttt                                                      >  2:150447/1‑93 (MQ=255)

                                  gtATCCCGCCTGTTTCAGCAAGGCTTTTGCCATCGCGACGCGCTCACTCATTGGCCTTTGCAGTTCATCGAACGTCGTCGCGCTAAAGCCttt                                                      >  1:4454828/1‑93 (MQ=255)

                                   tATCCCGCCTGTTTCAGCAAGGCTTTTGCCATCGCGACGCGCTCACTCATTGGCCTTTGCAGTTCATCGAACGTCGTCGCGCTAAAGCCtttt                                                     >  1:2724175/1‑93 (MQ=255)

                                    aTCCCGCCTGTTTCAGCAAGGCTTTTGCCATCGCGACGCGCTCACTCATTGGCCTTTGCAGTTCATCGAACGTCGTCGCGCTAAAGCCTTTTa                                                    >  1:3763149/1‑93 (MQ=255)

                                    aTCCCGCCTGTTTCAGCAAGGCTTTTGCCATCGCGACGCGCTCACTCATTGGCCTTTGCAGTTCATCGAACGTCGTCGCGCTAAAGCCTTTTa                                                    <  1:4348420/93‑1 (MQ=255)

                                    aTCCCGCCTGTTTCAGCAAGGCTTTTGCCATCGCGACGCGCTCACTCATTGGCCTTTGCAGTTCATCGAACGTCGTCGCGCTAAAGACTTTTa                                                    >  2:3231911/1‑93 (MQ=255)

                                     tCCCGCCTGTTTCAGCAAGGCTTTTGCCATCGCGACGCGCTCACTCATTGGCCTTTGCAGTTCATCGAACGTCGTCGCGCTAAAGCCTTTTAc                                                   >  1:1282988/1‑93 (MQ=255)

                                     tCCCGCCTGTTTCAGCAAGGCTTTTACCATCGCGACGCGCTCACTCATTGGCCTTTGCAGTTCATCGAACGTCGTCGCGCTAAAGCCTTTTAc                                                   >  2:230402/1‑93 (MQ=255)

                                      cccGCCTGTTTCAGCAAGGCTTTTGCCATCGCGACGCGCTCACTCATTGGCCTTTGCAGTTCATCGAACGTCGTCGCGCTAAAGCCTTTTAcc                                                  >  2:3627209/1‑93 (MQ=255)

                                       ccGCCTGTTTCAGCAAGGCTTTTGCCATCGCGACGCGCTCACTCATTGGCCTTTGCAGTTCATCGAACGTCGTCGCGCTAAAGCCTTTTACct                                                 >  2:3603972/1‑93 (MQ=255)

                                        cGCCTGTTTCAGCAAGGCTTTTGCCATCGCGACGCGCTCACTCATTGGCCTTTGCAGTTCATCGAACGTCGTCGCGCTAAAGCCTTTTACctc                                                >  2:2886425/1‑93 (MQ=255)

                                         gCCTGTTTCAGCAAGGCTTTTGCCATCGCGACGCGCTCACTCATTGGCCTTTGCAGTTCATCGAACGTCGTCGCGCTAAAGCCTTTTACctct                                               <  1:1594622/93‑1 (MQ=255)

                                         cccTGTTTCAGCAAGGCTTTTGCCATCGCGACGCGCTCACTCATTGGCCTTTGCAGTTCATCGAACGTCGTCGCGCTAAAGCCTTTTACctct                                               <  2:2265006/92‑1 (MQ=255)

                                          ccTGTTTCAGCAAGGCTTTTGCCATCGCGACGCGCTCACTCATTGGCCTTTGCAGTTCATCGAACGTCGTCGCGCTAAAGCCTTTTACCTCTg                                              >  1:4495602/1‑93 (MQ=255)

                                          ccTGTTTCAGCAAGGCTTTTGCCATCGCGACGCGCTCACTCATTGGCCTTTGCAGTTCATCGAACGTCGTCGCGCTAAAGCCTTTTACCTCTg                                              >  1:90238/1‑93 (MQ=255)

                                           cTGTTTCAGCAAGGCTTGTGCCATCGCGACGCGCTCACTCATTGGCCTTTGCAGTTCATCGAACGTCGTCGCGCTAAAGCCTTTTACCTCTgg                                             <  1:707533/93‑1 (MQ=255)

                                             gTTTCAGCAAGGCTTTTGCCATCGCGACGCGCTCACTCATTGGCCTTTGCAGTTCATCGAACGTCGTCGCGCTAAAGCCTTTTACCTCTggcg                                           >  2:87593/1‑93 (MQ=255)

                                             gTTTCAGCAAGGCTTTTGCCATCGCGACGCGCTCACTCATTGGCCTTTGCAGTTCATCGAACGTCGTCGCGCTAAAGCCTTTTACCTCTggcg                                           >  2:3430336/1‑93 (MQ=255)

                                             gTTTCAGCAAGGCTTTTGCCATCGCGACGCGCTCACTCATTGGCCTTTGCAGTTCATCGAACGTCGTCGCGCTAAAGCCTTTTACCTCTggcg                                           >  2:2830969/1‑93 (MQ=255)

                                              tttCAGCAAGGCTTTTGCCATCGCGACGCGCTCACTCATTGGCCTTTGCAGTTCATCGAACGTCGTCGGGCTAAAGACTTTTCCCTCTggcgg                                          >  1:3575375/1‑93 (MQ=255)

                                              tttCAGCAAGGCTTTTGCCATCGCGACGCGCTCACTCATTGGCCTTTGCAGTTCATCGAACGTCGTCGCGCTAAAGCCTTTTACCTCTggcgg                                          <  2:868997/93‑1 (MQ=255)

                                              tttCAGCAAGGCTTTTGCCATCGCGACGCGCTCACTCATTGGCCTTTGCAGTTCATCGAACGTCGTCGCGCTAAAGCCTTTTACCTCTggcgg                                          >  2:1809904/1‑93 (MQ=255)

                                                 cAGCAAGGCTTTTGCCATCGCGACGCGCTCACTCATTGGCCTTTGCAGTTCATCGAACGTCGTCGCGCTAAAGCCTTTTACCTCTGGCGGCGt                                       >  2:360007/1‑93 (MQ=255)

                                                  aGCAAGGCTTTTGCCATCGCGACGCGCTCACTCATTGGCCTTTGCAGTTCATCGAACGTCGTCGCGCTAAAGCCTTTTACCTCTGGCGGCGTc                                      >  2:3821392/1‑93 (MQ=255)

                                                   gCAAGGCTTTTGCCATCGCGACGCGCTCACTCATTGGCCTTTGCAGTTCATCGAACGTCGTCGCGCTAAAGCCTTTTACCTCTGGCGGCGTCa                                     <  2:3290050/93‑1 (MQ=255)

                                                     aaGGCTTTTGCCATCGCGACGCGCTCACTCATTGGCCTTTGCAGTTCATCGAACGTCGTCGCGCTAAAGCCTTTTACCTCTGGCGGCGTCAGc                                   >  2:1853408/1‑93 (MQ=255)

                                                      aGGCTTTTGCCATCGCGACGCGCTCACTCATTGGCCTTTGCAGTTCATCGAACGTCGTCGCGCTAAAGCCTTTTACCTCTGGCGGCGTCAGCg                                  >  1:3575439/1‑93 (MQ=255)

                                                      aGGCTTTTGCCATCGCGACGCGCTCACTCATTGGCCTTTGCAGTTCATCGAACGTCGTCGCGCTAAAGCCTTTTACCTCTGGCGGCGTCAGCg                                  >  1:4369349/1‑93 (MQ=255)

                                                       ggCTTTTGCCATCGCGACGCGCTCACTCATTGGCCTTTGCAGTTCATCGAACGTCGTCGCGCTAAAGCCTTTTACCTCTGGCGGCGTCAGCgg                                 >  1:2774743/1‑92 (MQ=255)

                                                           tttGCCATCGCGACGCGCTCACTCATTGGCCTTTGCAGTTCATCGAACGTCGTCGCGCTAAAGCCTTTTACCTCTGGCGGCGTCAGCGTGGtt                             >  1:1713370/1‑93 (MQ=255)

                                                                cATCGCGACGCGCTCACTCTTTGGCCTTTGCAGTTCATCGAACGTCGTCGCGCTAAAGCCTTTTACCTCTGGCGGCGTCAGCGTGGTTGCggg                        >  2:4572178/1‑93 (MQ=255)

                                                                cATCGCGACGCGCTCACTCATTGGCCTTTGCAGTTCATCGAACGTCGTCGCGCTAAAGCCTTTTACCTCTGGCGGCGTCAGCGTGGTTGCggg                        <  1:3210661/93‑1 (MQ=255)

                                                                 aTCGCGACGCGCTCACTCATTGGCCTTTGCAGTTCATCGAACGTCGTCGCGCTAAAGCCTTTTACCTCTGGCGGCGTCAGCGTGGTTGCGGGc                       >  1:2004530/1‑93 (MQ=255)

                                                                 aTCGCGACGCGCTCACTCATTGGCCTTTGCAGTTCATCGAACGTCGTCGCGCTAAAGCCTTTTACCTCTGGCGGCGTCAGCGTGGTTGCGGGc                       >  2:1087449/1‑93 (MQ=255)

                                                                  tCGCGACGCGCTCACTCATTGGCCTTTGCAGTTCATCGAACGTCGTCGCGCTAAAGCCTTTTACCTCTGGCGGCGTCAGCGTGGTTGCGGGCg                      >  2:1330733/1‑93 (MQ=255)

                                                                  tCGCGACGCGCTCACTCATTGGCCTTTGCAGTTCATCGAACGTCGTCGCGCTAAAGCCTTTTACCTCTGGCGGCGTCAGCGTGGTTGCGGGCg                      <  2:1772803/93‑1 (MQ=255)

                                                                    gcgACGCGCTCACTCATTGGCCTTTGCAGTTCATCGAACGTCGTCGCGCTAAAGCCTTTTACCTCTGGCGGCGTCAGCGTGGTTGCGGGCGtt                    >  1:2654450/1‑93 (MQ=255)

                                                                      gACGCGCTCACTCATTGGCCTTTGCAGTTCATCGAACGTCGTCGCGCTACAGCCTTTTACCTCTGGCGGCGTCAGCGTGGTTGCGGGCGTtct                  >  2:2844114/1‑93 (MQ=255)

                                                                      gACGCGCTCACTCATTGGCCTTTGCAGTTCATCGAACGTCGTCGCGCTAAAGCCTTTTACCTCTGGCGGCGTCAGCGTGGTTGCGGGCGTtct                  <  2:1154382/93‑1 (MQ=255)

                                                                       aCGCGCTCACTCATTGGCCTTTGCAGTTCATCGAACGTCGTCGCGCTAAAGCCTTTTACCTCTGGCGGCGTCAGCGTGGTTGCGGGCGTtctc                 >  1:1320461/1‑93 (MQ=255)

                                                                       aCGCGCTCACTCATTGGCCTTTGCAGTTCATCGAACGTCGTCGCGCTAAAGCCTTTTACCTCTGGCGGCGTCAGCGTGGTTGCGGGCGTtctc                 <  2:4401988/93‑1 (MQ=255)

                                                                         gcgcTCACTCATTGGCCTTTGCAGTTCATCGAACGTCGTCGCGCTAAAGCCTTTTACCTCTGGCGGCGTCAGCGTGGTTGCGGGCGTTCTCaa               >  2:2944724/1‑93 (MQ=255)

                                                                         gcgcTCACTCAGTGGCCTTTGCAGTTCATCGAACGTCGTCGCGCTAAAGCCTTTTACCTCTGGCGGCGTCAGCGTGGTTGCGGGCGTTCTCaa               <  2:735656/93‑1 (MQ=255)

                                                                           gcTCACTCTTTGGCCTTTGCGGTTCCTCGCCCGTCGTCGCGCTACCGCCTTTTACCTCTGGCGGCGTCCGCGTGGTTGCGGGCGTTCTCAccc             <  1:4687077/93‑1 (MQ=255)

                                                                            ctcactcaTTGGCCTTTGCAGTTCATCGAACGTCGTCGCGCTAAAGCCTTTTACCTCTGGCGGCGTCAGCGTGGTTGCGGGCGTTCTCAAccc            >  2:2466545/1‑93 (MQ=255)

                                                                             tcactcaTTGGCCTTTGCAGTTCATCGAACGTCGTCGCGCTAAAGCCTTTTACCTCTGGCGGCGTCAGCGTGGTTGCGGGCGTTCTCAAcccc           >  1:142915/1‑93 (MQ=255)

                                                                              cactcaTTGGCCTTTGCAGTTCATCGAACGTCGTCGCGCTAAAGCCTTTTACCTCTGGCGGCGTCAGCGTGGTTGCGGGCGTTCTCAACCCCa          >  1:2922553/1‑93 (MQ=255)

                                                                              cactcaTTGGCCTTTGCAGTTCATCGAACGTCGTCGCGCTAAAGCCTTTTACCTCTGGCGGCGTCAGCGTGGTTGCGGGCGTTCTCAACCCCa          >  1:3620452/1‑93 (MQ=255)

                                                                               actcaTTGGCCTTTGCAGTTCATCGAACGTCGTCGCGCTAAAGCCTTTTACCTCTGGCGGCGTCAGCGTGGTTGCGGGCGTTCTCAACCCCAg         <  1:834270/93‑1 (MQ=255)

                                                                               actcaTTGGCCTTTGCAGTTCATCGAACGTCGTCGCGCTAAAGCCTTTTACCTCTGGCGGCGTCAGCGTGGTTGCGGGCGTTCTCAACCCCAg         <  2:4209514/93‑1 (MQ=255)

                                                                               actcaTTGGCCTTTGCAGTTCATCGAACGTCGTCGCGCTAAAGCCTTTTACCTCTGGCGGCGTCAGCGTGGTTGCGGGCGTTCTCAACCCCAg         >  1:1764442/1‑93 (MQ=255)

                                                                               actcaTTGGCCTTTGCAGTTCATCGAACGTCGTCGCGCTAAAGCCTTTTACCTCTGGCGGCGTCAGCGTGGTTGCGGGCGTTCTCAACCCCAg         >  1:180239/1‑93 (MQ=255)

                                                                               actcaTTGGCCTTTGCAGTTCATCGAACGTCGTCGCGCTAAAGCCTTTTACCTCTGGCGGCGTCAGCGTGGTTGCGGGCGTTCTCAACCCCAg         >  1:3452969/1‑93 (MQ=255)

                                                                                ctcaTTGGCCTTTGCAGTTCATCGAACGTCGTCGCGCTAAAGCCTTTTACCTCTGGCGGCGTCAGCGTGGTTGCGGGCGTTCTCAACCCCAGt        <  2:4267524/93‑1 (MQ=255)

                                                                                ctcaTTGGCCTTTGCAGTTCATCGAACGTCGTCGCGCTAAAGCCTTTTACCTCTGGCGGCGTCAGCGTGGTTGCGGGCGTTCTCAACCCCAGt        >  2:3505536/1‑93 (MQ=255)

                                                                                  caTTGGCCTTTGCAGTTCATCGAACGTCGTCGCGCTAAAGCCTTTTACCTCTGGCGGCGTCAGCGTGGTTGCGGGCGTTCTCAACCCCAGTAc      <  2:2079059/93‑1 (MQ=255)

                                                                                  caTTGGCCTTTGCAGTTCATCGAACGTCGTCGCGCTAAAGCCTTTTACCTCTGGCGGCGTCAGCGTGGTTGCGGGCGTTCTCAACCCCAGTAc      <  2:804797/93‑1 (MQ=255)

                                                                                    ttGGCCTTTGCAGTTCATCGAACGTCGTCGCGCTAAAGCCTTTTACCTCTGGCGGCGTCAGCGTGGTTGCGGGCGTTCTCAACCCCAGTACCt    >  2:1429191/1‑93 (MQ=255)

                                                                                    ttGGCCTTTGCAGTTCATCGAACGTCGTCGCGCTAAAGCCTTTTACCTCTGGCGGCGTCAGCGTGGTTGCGGGCGTTCTCAACCCCAGTACCt    >  1:4147314/1‑93 (MQ=255)

                                                                                    ttGGCCTTTGCAGTTCATCGAACGTCGTCGCGCTAAAGCCTTTTACCTCTGGCGGCGTCAGCGTGGTTGCGGGCGTTCTCAACCCCAGTACCt    <  2:2393671/93‑1 (MQ=255)

                                                                                     tGGCCTTTGCAGTTCATCGAACGTCGTCGCGCTAAAGCCTTTTACCTCTGGCGGCGTCAGCGTGGTTGCGGGCGTTCTCAACCCCAGTACCtt   <  1:1994574/93‑1 (MQ=255)

                                                                                     tGGCCTTTGCAGTTCATCGAACGTCGTCGCGCTAAAGCCTTTTACCTCTGGCGGCGTCAGCGTGGTTGCGGGCGTTCTCAACCCCAGTACCtt   >  1:3106203/1‑93 (MQ=255)

                                                                                      ggCCTTTGCAGTTCATCGAACGTCGTCGCGCTAAAGCCTTTTACCTCTGGCGGCGTCAGCGTGGTTGCGGGCGTTCTCAACCCCAGTACCttt  <  1:4269625/93‑1 (MQ=255)

AGAACAGCTCAAAGCGTAGCGGATGAGAGGCGTCGTATCCCGCCTGTTTCAGCAAGGCTTTTGCCATCGCGACGCGCTCACTCATTGGCTTTTGCAGTTCATCGAACGTCGTCGCGCTAAAGCCTTTTACCTCTGGCGGCGTCAGCGTGGTTGCGGGCGTTCTCAACCCCAGTACCTTT  >  NC_000913/3164588‑3164766

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 19 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`