BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,212,918	A→G	V66A (GTC→GCC)	ygjF ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,212,918	0	A	G	99.4%	488.7 / NA	157	V66A (GTC→GCC)	ygjF	hypothetical protein
Reads supporting (aligned to +/- strand): ref base A (0/0); major base G (77/79); minor base T (0/1); total (77/80)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 169 total aligned reads displayed.

TTTTTTCAATCAGCTTACGCCCGCCTGCGTGTAGCTCCTGCTTTGAAACTTCATTGGCTTGCACCGTTGGACGGTCTACCAGTTTGGTGACGCCACAACGATAATCCAGCAGATGCTGTGCCTCCTGCGGCTTCAACTGACGGTCGGTAAACCCGGCCTGATATATCACCTTCCAGAAG  >  NC_000913/3212829‑3213007

ttttttCAATCAGCTTACGCCCGCCTGCGTGTAGCTCCTGCTTTGAAACTTCATTGGCTTGCACCGTTGGACGGTCTACCAGTTTGGTGgcgc                                                                                        >  2:3086448/1‑93 (MQ=255)

    ttCAATCAGCTTACGCCCGCCTGCGTGTAGCTCCTGCTTTGAAACTTCATTGGCTTGCACCGTTGGACGGTCTACCAGTTTGGTGGCGCcaca                                                                                    <  2:4365546/93‑1 (MQ=255)

     tCAATCAGCTTACGCCCGCCTGCGTGTAGCTCCTGCTTTGAAACTTCATTGGCTTGCACCGTTGGACGGTCTACCAGTTTGGTGGCGCCACaa                                                                                   <  2:1661388/93‑1 (MQ=255)

     tCAATCAGCTTACGCCCGCCTGCGTGTAGCTCCTGCTTTGAAACTTCATTGGCTTGCACCGTTGGACGGTCTACCAGTTTGGTGGCGCCACaa                                                                                   <  1:4373321/93‑1 (MQ=255)

     tCAATCAGCTTACGCCCGCCTGCGTGTAGCTCCTGCTTTGAAACTTCATTGGCTTGCACCGTTGGACGGTCTACCAGTTTGGTGGCGCCACaa                                                                                   <  2:4004067/93‑1 (MQ=255)

     tCAATCAGCTTACGCCCGCCTGCGTGTAGCTCCTGCTTTGAAACTTCATTGGCTTGCACCGTTGGACGGTCTACCAGTTTGGTGGCGCCACaa                                                                                   <  2:4274324/93‑1 (MQ=255)

       aaTCAGCTTACGCCCGCCTGCGTGTAGCTCCTGCTTTGAAACTTCATTGGCTTGCACCGTTGGACGGTCTACCAGTTTGGTGGCGCCACAACg                                                                                 >  1:3409323/1‑93 (MQ=255)

        aTCAGCTTACGCCCGCCTGCGTGTAGCTCCTGCTTTGAAACTTCATTGGCTTGCACCGTTGGACGGTCTACCAGTTTGGTGGCGCCACAACGa                                                                                >  1:747159/1‑93 (MQ=255)

        aTCAGCTTACGCCCGCCTGCGTGTAGCTCCTGCTTTGAAACTTCATTGGCTTGCACCGTTGGACGGTCTACCAGTTTGGTGGCGCCACAACGa                                                                                <  1:2199259/93‑1 (MQ=255)

         tCAGCTTACGCCCGCCTGCGTGTAGCTCCTGCTTTGAAACTTCATTGGCTTGCACCGTTGGACGGTCTACCAGTTTGGTGGCGCCACAACGAt                                                                               >  2:1423887/1‑93 (MQ=255)

         tCAGCTTACGCCCGCCTGCGTGTAGCTCCTGCTTTGAAACTTCATTGGCTTGCACCGTTGGACGGTCTACCAGTTTGGTGGCGCCACAACGAt                                                                               >  2:385987/1‑93 (MQ=255)

         tCAGCTTACGCCCGCCTGCGTGTAGCTCCTGCTTTGAAACTTCATTGGCTTGCACCGTTGGACGGTCTACCAGTTTGGTGGCGCCACAACGAt                                                                               <  1:1951566/93‑1 (MQ=255)

         tCAGCTTACGCCCGCCTGCGTGTAGCTCCTGCTTTGAAACTTCATTGGCTTGCACCGTTGGACGGTCTACCAGTTTGGTGGCGCCACAACGAt                                                                               <  1:206739/93‑1 (MQ=255)

           aGCTTACGCCCGCCTGCGTGTAGCTCCTGCTTTGAAACTTCATTGGCTTGCACCGTTGGACGGTCTACCAGTTTGGTGGCGCCACAACGATaa                                                                             >  2:1744800/1‑93 (MQ=255)

              ttACGCCCGCCTGCGTGTAGCTCCTGCTTTGAAACTTCATTGGCTTGCACCGTTGGACGGTCTACCAGTTTGGTGGCGCCACAACGATAATcc                                                                          >  1:1308894/1‑93 (MQ=255)

              ttACGCCCGCCTGCGTGTAGCTCCTGCTTTGAAACTTCATTGGCTTGCACCGTTGGACGGTCTACCAGTTTGGTGGCGCCACAACGATAATcc                                                                          <  1:2879415/93‑1 (MQ=255)

              ttACGCCCGCCTGCGTGTAGCTCCTGCTTTGAAACTTCATTGGCTTGCACCGTTGGACGGTCTACCAGTTTGGTGGCGCCACAACGATAATcc                                                                          >  1:296268/1‑93 (MQ=255)

              ttACGCCCGCCTGCGTGTAGCTCCTGCTTTGAAACTTCATTGGCTTGCACCGTTGGACGGTCTACCAGTTTGGTGGCGCCACAACGATAATcc                                                                          <  1:3256000/93‑1 (MQ=255)

              ttACGCCCGCCTGCGTGTAGCTCCTGCTTTGAAACTTCATTGGCTTGCACCGTTGGACGGTCTACCAGTTTGGTGGCGCCACAACGATAATcc                                                                          >  2:4557679/1‑93 (MQ=255)

               tACGCCCGCCTGCGTGTAGCTCCTGCTTTGAAACTTCATTGGCTTGCACCGTTGGACGGTCTACCAGTTTGGTGGCGCCACAACGATAATCca                                                                         >  1:2899661/1‑93 (MQ=255)

                  gcccgccTGCGTGTAGCTCCTGCTTTGAAACTTCATTGGCTTGCACCGTTGGACGGTCTACCAGTTTGGTGGCGCCACAACGATAATCcagca                                                                      <  1:4337954/93‑1 (MQ=255)

                  gcccgccTGCGTGTAGCTCCTGCTTTGAAACTTCATTGGCTTGCACCGTTGGACGGTCTACCAGTTTGGTGGCGCCACAACGATAATCcagca                                                                      <  2:2974555/93‑1 (MQ=255)

                   cccgccTGCGTGTAGCTCCTGCTTTGAAACTTCATTGGCTTGCACCGTTGGACGGTCTACCAGTTTGGTGGCGCCACAACGATAATCcagcag                                                                     <  2:2069940/93‑1 (MQ=255)

                   cccgccTGCGTGTAGCTCCTGCTTTGAAACTTCATTGGCTTGCACCGTTGGACGGTCTACCAGTTTGGTGGCGCCACAACGATAATCcagcag                                                                     >  1:3256243/1‑93 (MQ=255)

                   cccgccTGCGTGTAGCTCCTGCTTTGAAACTTCATTGGCTTGCACCGTTGGACGGTCTACCAGTTTGGTGGCGCCACAACGATAATCcagcag                                                                     >  2:3116560/1‑93 (MQ=255)

                    ccgccTGCGTGTAGCTCCTGCTTTGAAACTTCATTGGCTTGCACCGTTGGACGGTCTACCAGTTTGGTGGCGCCACAACGATAATCCAGCAGa                                                                    >  1:3032221/1‑93 (MQ=255)

                    ccgccTGCGTGTAGCTCCTGCTTTGAAACTTCATTGGCTTGCACCGTTGGACGGTCTACCAGTTTGGTGGCGCCACAACGATAATCCAGCAGa                                                                    >  2:4592553/1‑93 (MQ=255)

                    ccgccTGCGTGTAGCTCCTGCTTTGAAACTTCATTGGCTTGCACCGTTGGACGGTCTACCAGTTTGGTGGCGCCACAACGATAATCCAGCAGa                                                                    <  1:368648/93‑1 (MQ=255)

                     cgccTGCGTGTAGCTCCTGCTTTGAAACTTCATTGGCTTGCACCGTTGGACGGTCTACCAGTTTGGTGGCGCCACAACGATAATCCAGCAGAt                                                                   <  2:3381727/93‑1 (MQ=255)

                      gccTGCGTGTAGCTCCTGCTTTGAACCTTCATTGGCTTGCACCGTTGGACGGTCTACCAGTTTGGTGGCGCCACAACGATAATCCAGCAGATg                                                                  <  2:2621257/93‑1 (MQ=255)

                       ccTGCGTGTAGCTCCTGCTTTGAAACTTCATTGGCTTGCACCGTTGGACGGTCTACCAGTTTGGTGGCGCCACAACGATAATCCAGCAGATGc                                                                 <  2:3835691/93‑1 (MQ=255)

                       ccTGCGTGTAGCTCCTGCTTTGAAACTTCATTGGCTTGCACCGTTGGACGGTCTACCAGTTTGGTGGCGCCACAACGATAATCCAGCAGATGc                                                                 >  2:2547796/1‑93 (MQ=255)

                        cTGCGTGTAGCTCCTGCTTTGAAACTTCATTGGCTTGCACCGTTGGACGGTCTACCAGTTTGGTGGCGCCACAACGATAATCCAGCAGATGCt                                                                <  2:2066554/93‑1 (MQ=255)

                         tGCGTGTAGCTCCTGCTTTGAAGCTTCATTGGCTTGCACCGTTGGACGGTCTACCAGTTTGGTGTCGCCACAACGATAATCCAGCAGATGCtg                                                               <  2:1075136/93‑1 (MQ=255)

                         tGCGTGTAGCTCCTGCTTTGAAACTTCATTGGCTTGCACCGTTGGACGGTCTACCAGTTTGGTGGCGCCACAACGATAATCCAGCAGATGCtg                                                               >  1:4070213/1‑93 (MQ=255)

                          gCGTGTAGCTCCTGCTTTGAAACTTCATTGGCTTGCACCGTTGGACGGTCTACCAGTTTGGTGGCGCCACAACGATAATCCAGCAGATGCtgt                                                              >  2:631034/1‑93 (MQ=255)

                             tgtAGCTCCTGCTTTGAAACTTCATTGGCTTGCACCGTTGGACGGTCTACCAGTTTGGTGGCGCCACAACGATAATCCAGCAGATGCTGTGcc                                                           >  1:3131840/1‑93 (MQ=255)

                             tgtAGCTCCTGCTTTGAAACTTCATTGGCTTGCACCGTTGGACGGTCTACCAGTTTGGTGGCGCCACAACGATAATCCAGCAGATGCTGTGcc                                                           >  1:2168326/1‑93 (MQ=255)

                              gtAGCTCCTGCTTTGAAACTTCATTGGCTTGCACCCTTGGACGGTCTACCAGTTTGGTGGCGCCACAACGATAATCCAGCAGATGATGTGcgc                                                          >  2:4100571/1‑91 (MQ=255)

                               tAGCTCCTGCTTTGAAACTTCATTGGCTTGCACCGTTGGACGGTCTACCCGTTTGGTGGCGCCACAACGATAATCCAACAGATGCTGTGcctc                                                         >  2:2379220/1‑93 (MQ=255)

                               tAGCTCCTGCTTTGAAACTTCATTGGCTTGCACCGTTGGACGGTCTACCAGTTTGGTGGCGCCACAACGATAATCCAGCAGATGCTGTGcctc                                                         >  1:521530/1‑93 (MQ=255)

                                 gCTCCTGCTTTGAAACTTCATTGGCTTGCACCGTTGGACGGTCTACCAGTTTGGTGGCGCCACAACGATAATCCAGCAGATGCTGTGcctcct                                                       <  1:1345194/93‑1 (MQ=255)

                                  cTCCTGCTTTGAATCTTCATTGGCTTGCACCGTTGGACGGTCTACCAGTTTGGTGGCGCCACAACGATAATCCAGCAGATGCTGTGGCTCCTg                                                      >  2:107691/1‑93 (MQ=255)

                                  cTCCTGCTTTGAAACTTCATTGGCTTGCACCGTTGGACGGTCTACCAGTTTGGTGGCGCCACAACGATAATCCAGCAGATGCTGTGCCTCCTg                                                      <  1:2707138/93‑1 (MQ=255)

                                  cTCCTGCTTTGAAACTTCATTGGCTTGCACCGTTGGACGGTCTACCAGTTTGGTGGCGCCACAACGATAATCCAGCAGATGCTGTGCCTCCTg                                                      <  1:827940/93‑1 (MQ=255)

                                  cTCCTGCTTTGAAACTTCATTGGCTTGCACCGTTGGACGGTCTACCAGTTTGGTGGCGCCACAACGATAATCCAGCAGATGCTGTGCCTCCTg                                                      <  1:1292613/93‑1 (MQ=255)

                                   tCCTGCTTTGAAACTTCATTGGCTTGCACCGTTGGACGGTCTACCAGTTTGGTGGCGCCACAACGATAATCCAGCAGATGCTGTGCCTCCTgg                                                     >  2:433289/1‑92 (MQ=255)

                                   tCCTGCTTTGAAACTTCATTGGCTTGCACCGTTGGACGGTCTACCAGTTTGGTGGCGCCACAACGATAATCCAGCAGATGCTGTGCCTCCTGc                                                     >  1:3974565/1‑93 (MQ=255)

                                   tCCTGCTTTGAAACTTCATTGGCTTGCACCGTTGGACGGTCTACCAGTTTGGTGGCGCCACAACGATAATCCAGCAGATGCTGTGCCTCCTGc                                                     >  1:1212461/1‑93 (MQ=255)

                                   tCCTGCTTTGAAACTTCATTGGCTTGCACCGTTGGACGGGCTACCCGATTGGTGGCGCCACAACGATAATTCAGCAGACGCTGTGCctgctgg                                                     >  2:3301376/1‑92 (MQ=255)

                                    ccTGCTTTGAAACTTCATTGGCTTGCACCGTTGGACGGTCTACCAGTTTGGTGGCGCCACAACGATAATCCAGCAGATGCTGTGCCTCCTGCg                                                    <  1:4229472/93‑1 (MQ=255)

                                     cTGCTTTGAAACTTCATTGGCTTGCACCGTTGGACGGTCTACCAGTTTGGTGGCGCCACAACGATAATCCAGCAGATGCTGTGCCTCCTGCgg                                                   >  2:3534/1‑93 (MQ=255)

                                     cTGCTTTGAAACTTCATTGGCTTGCACCGTTGGACGGTCTACCAGTTTGGTGGCGCCACAACGATAATCCAGCAGATGCTGTGCCTCCTGCgg                                                   <  2:4016273/93‑1 (MQ=255)

                                      tGCTTTGAAACTTCATTGGCTTGCACCGTTGGACGGTCTACCAGTTTGGTGGCGCCACAACGATAATCCAGCAGATGCTGTGCCTCCTGCGGc                                                  <  2:1011791/93‑1 (MQ=255)

                                      tGCTTTGAAACTTCATTGGCTTGCACCGTTGGACGCTCTACCAGTTTGGTGGCGCCACAACGATAATCCAGCAGATGCTGTGCCTCCTGCGGc                                                  <  2:869374/93‑1 (MQ=255)

                                       gCTTTGAAACTTCATTGGCTTGCACCGTTGGACGGTCTACCAGTTTGGTGGCGCCACAACGATAATCCAGCAGATGCTGTGCCTCCTGCGGCt                                                 >  2:1230215/1‑93 (MQ=255)

                                       gCTTTGAAACTTCATTGGCTTGCACCGTTGGACGGTCTACCAGTTTGGTGGCGCCACAACGATAATCCAGCAGATGCTGTGCCTCCTGCGGCt                                                 >  1:1999963/1‑93 (MQ=255)

                                       gCTTTGAAACTTCATTGGCTTGCACCGTTGGACGGTCTACCAGTTTGGTGGCGCCACAACGATAATCCAGCAGATGCTGTGCCTCCTGCGGCt                                                 <  1:4524592/93‑1 (MQ=255)

                                          ttGAAACTTCATTGGCTTGCACCGTTGGACGGTCTACCAGTTTGGTGGCGCCACAACGATAATCCAGCAGATGCTGTGCCTCCTGCGGCTTCa                                              >  2:2838574/1‑93 (MQ=255)

                                           tGAAACTTCATTGGCTTGCACCGTTGGACGGTCTACCAGTTTGGTGGCGCCACAACGATAATCCAGCAGATGCTGTGCCTCCTGCGGCTTCaa                                             >  1:1912626/1‑93 (MQ=255)

                                            gAAACTTCATTGGCTTGCACCGTTGGACGGTCTACCAGTTTGGTGGCGCCACAACGATAATCCAGCAGATGCTGTGCCTCCTGCGGCTTCAAc                                            >  2:3973999/1‑93 (MQ=255)

                                            gAAACTTCATTGGCTTGCACCGTTGGACGGTCTACCAGTTTGGTGGCGCCACAACGATAATCCAGCAGATGCTGTGCCTCCTGCGGCTTCAAc                                            <  1:4538064/93‑1 (MQ=255)

                                            gAAACTTCATTGGCTTGCACCGTTGGACGGTCTACCAGTTTGGTGGCGCCACAACGATAATCCAGCAGATGCTGTGCCTCCTGCGGCTTCAAc                                            <  2:2341960/93‑1 (MQ=255)

                                            gAAACTTCATTGGCTTGCACCGTTGGACGGTCTACCAGTTTGGTGGCGCCACAACGATAATCCAGCAGATGCTGTGCCTCCTGCGGCTTCAAc                                            <  1:608816/93‑1 (MQ=255)

                                              aaCTTCATTGGCTTGCACCGTTGGACGGTCTACCAGTTTGGTGGCGCCACAACGATAATCCAGCAGATGCTGTGCCTCCTGCGGATTGAACTg                                          >  1:1965043/1‑93 (MQ=255)

                                              aaCGTCATTGGCTTGCACCGTTGGACGGTCTACCAGTTTGGTGGCGCCACAACGATAATCCAGCAGATGCTGTGCCTCCTGCGGCTTCAACTg                                          <  1:1847145/93‑1 (MQ=255)

                                               aCTTCATTGGCTTGCACCGTTGGACGGTCTACCAGTTTGGTGGCGCCACAACGATAATCCAGCAGATGCTGTGCCTCCTGCGGCTTCAACTGa                                         >  2:4427987/1‑93 (MQ=255)

                                                cTTCATTGGCTTGCACCGTTGGACGGTCTACCAGTTTGGTGGCGCCACAACGATAATCCAGCAGATGCTGTGCCTCCTGCGGCTTCAACTGAc                                        <  2:1861517/93‑1 (MQ=255)

                                                  tcgttGGCTTGCGCCGTTGGACGGTCTACCAGTTTGGTGGCGCCACAAAGATAATCCAGCAGAGGCTGTCCCTCCTGCGGCTTCAACTGAcgg                                      <  2:2299906/90‑1 (MQ=255)

                                                  tCATTGGCTTGCACCGTTGGACGGTCTACCAGTTTGGTGGCGCCACAACGATAATCCAGCAGATGCTGTGCCTCCTGCGGCTTCAACTGAcgg                                      <  1:4123925/93‑1 (MQ=255)

                                                   cATTGGCTTGCCCCGTTTGACGGTCTACCAGTTTGGTGGCGCCACAACGATAATCCAGCAGATGCTGGGCCTCCTGCGGCTTCAACTGAcggt                                     <  1:4144737/93‑1 (MQ=255)

                                                   cATTGGCTTGCACCGTTGGACGGTCTACCAGTTTGGTGGCGCCACAACGATAATCCAGCAGATGCTGTGCCTCCTGCGGCTTCAACTGAcggt                                     <  2:2634601/93‑1 (MQ=255)

                                                   cATTGGCTTGCACCGTTGGACGGTCTACCAGTTTGGTGGCGCCACAACGATAATCCAGCAGATGCTGTGCCTCCTGCGGCTTCAACTGAcggt                                     >  1:3681112/1‑93 (MQ=255)

                                                    aTTGGCTTGCACCGTTGGACGGTCTACCAGTTTGGTGGCGCCACAACGATAATCCAGCAGATGCTGTGCCTCCTGCGGCTTCAACTGAcggtc                                    >  1:2313517/1‑93 (MQ=255)

                                                     ttGGCTTGCACCGTTGGACGGTCTACCAGTTTGGTGGCGCCACAACGATAATCCAGCAGATGCTGTGCCTCCTGCGGCTTCAACTGAcggtcg                                   <  2:4141301/93‑1 (MQ=255)

                                                       ggCTTGCACCGTTGGACGGTCTACCAGTTTGGTGGCGCCACAACGATAATCCAGCAGATGCTGTGCCTCCTGCGGCTTCAACTGAcggtcggt                                 >  2:15186/1‑93 (MQ=255)

                                                          ttGCACCGTTGGACGGTCTACCAGTTTGGTGGCGCCACAACGATAATCCAGCAGATGCTGTGCCTCCTGCGGCTTCAACTGACGGTCGGTcaa                              >  2:4545263/1‑93 (MQ=255)

                                                          ttGCACCGTTGGACGGTCTACCAGTTTGGTGGCGCCACAACGATAATCCAGCAGATGCTGTGCCTCCTGCGGCTTCAACTGACGGTCGGTaaa                              >  1:1056658/1‑93 (MQ=255)

                                                          ttGCACCGTTGGACGGTCTACCAGTTTGGTGGCGCCACAACGATAATCCAGCAGATGCTGTGCCTCCTGCGGCTTCAACTGACGGTCGGTaaa                              >  1:2951659/1‑93 (MQ=255)

                                                           tGCACCGTTGGACGGTCTACCAGTTTGGTGGCGCCACAACGATAATCCAGCAGATGCTGTGCCTCCTGCGGCTTCAACTGACGGTCGGTAAAc                             >  2:531304/1‑93 (MQ=255)

                                                            gCACCGTTGGACGGTCTACCAGTTTGGTGGCGCCACAACGATAATCCAGCAGATGCTGTGCCTCCTGCGGCTTCAACTGACGGTCGGTAAAcc                            >  1:2877064/1‑93 (MQ=255)

                                                            gCACCGTTGGACGGTCTACCAGTTTGGTGGCGCCACAACGATAATCCAGCAGATGCTGTGCCTCCTGCGGCTTCAACTGACGGTCGGTAAAcc                            >  2:973748/1‑93 (MQ=255)

                                                            gCACCGTTGGACGGTCTACCAGTTTGGTGGCGCCACAACGATAATCCAGCAGATGCTGTGCCTCCTGCGGCTTCAACTGACGGTCGGTAAAcc                            >  1:65213/1‑93 (MQ=255)

                                                               ccGTTGGACGGTCTACCAGTTTGGTGGCGCCACAACGATAATCCAGCAGATGCTGTGCCTCCTGCGGCTTCAACTGACGGTCGGTAAACACgg                         >  2:684337/1‑93 (MQ=255)

                                                                 gTTGGACGGTCTACCAGTTTGGTGGCGCCACAACGATAATCCAGCAGATGCTGTGCCTCCTGCGGCTTCAACTGACGGTCGGTAAACCCGGcc                       <  1:3718414/93‑1 (MQ=255)

                                                                  ttGGACGGTCTACCAGTTTGGTGGCGCCACAACGATAATCCAGCAGATGCTGTGCCTCCTGCGGCTTCAACTGACGGTCGGTAAACCCGGCCt                      >  1:2998284/1‑93 (MQ=255)

                                                                     gACGGTCTACCAGTTTGGTGGCGCCACAACGATAATCCAGCAGATGCTGTGCCTCCTGCGGCTTCAACTGACGGTCGGTAAACCCGGCCTGat                   <  1:15394/93‑1 (MQ=255)

                                                                      aCGGTCTACCAGTTTGGTGGCGCCACAACGATAATCCAGCAGATGCTGTGCCTCCTGCGGCTTCAACTGACGGTCGGTAAACCCGGCCTGata                  >  2:3842223/1‑93 (MQ=255)

                                                                      aCGGTCTACCAGTTTGGTGGCGCCACAACGATAATCCAGCAGATGCTGTGCCTCCTGCGGCTTCAACTGACGGTCGGTAAACCCGGCCTGata                  <  2:434490/93‑1 (MQ=255)

                                                                         gTCTACCAGTTTGGTGGCGCCACAACGATAATCCAGCAGATGCTGTGCCTCCTGCGGCTTCAACTGACGGTCGGTAAACCCGGCCTGatatat               <  2:3065837/93‑1 (MQ=255)

                                                                         gTCTACCAGTTTGGTGGCGCCACAACGATAATCCAGCAGATGCTGTGCCTCCTGCGGCTTCAACTGACGGTCGGTAAACCCGGCCTGatatat               >  2:2889601/1‑93 (MQ=255)

                                                                           cTACCAGTTTGGTGGCGCCACAACGATAATCCAGCAGATGCTGTGCCTCCTGCGGCTTCAACTGACGGTCGGTAAACCCGGCCTGATATATCa             <  2:2210240/93‑1 (MQ=255)

                                                                            tACCAGTTTGGTGGCGCCACAACGATAATCCAGCAGATGCTGTGCCTCCTGCGGCTTCAACTGACGGTCGGTAAACCCGGCCTGATATATCAc            <  2:2696553/93‑1 (MQ=255)

                                                                              ccAGTTTGGTGGCGCCACAACGATAATCCAGCAGATGCTGTGCCTCCTGCGGCTTCAACTGACGGTCGGTAAACCCGGCCTGATATATCACCt          <  1:3157547/93‑1 (MQ=255)

                                                                              ccAGTTTGGTGGCGCCACAACGATAATCCAGCAGATGCTGTGCCTCCTGCGGCTTCAACTGACGGTCGGTAAACCCGGCCTGATATATCACCt          >  2:4321583/1‑93 (MQ=255)

                                                                               cAGTTTGGTGGCGGCACAACGATAATCCAGCAGATGCTGTGCCTCCTGCGGCTTCAACTGACGGTCGGTAAACCCGGCCTGATATATCACCtt         >  2:4606833/1‑93 (MQ=255)

                                                                                aGTTTGGTGGCGCCACAACGATAATCCAGCAGATGCTGTGCCTCCTGCGGCTTCAACTGACGGTCGGTAAACCCGGCCTGATATATCACCTTc        <  2:1330928/93‑1 (MQ=255)

                                                                                  tttGGTGGCGCCACAACGATAATCCAGCAGATGCTGTGCCTCCTGCGGCTTCAACTGACGGTCGGTAAACCCGGCCTGATATATCACCTTCCa      <  1:3511139/93‑1 (MQ=255)

                                                                                      gTGGCGCCACAACGATAATCCAGCAGATGCTGTGCCTCCTGCGGCTTCAACTGACGGTCGGTAAACCCGGCCTGATATATCACCTTCCAGAAg  <  1:920780/93‑1 (MQ=255)

                                                                                      gTGGCGCCACAACGATAATCCAGCAGATGCTGTGCCTCCTGCGGCTTCAACTGACGGTCGGTAAACCCGGCCTGATATATCACCTTCCAGAAg  <  2:3245487/93‑1 (MQ=255)

TTTTTTCAATCAGCTTACGCCCGCCTGCGTGTAGCTCCTGCTTTGAAACTTCATTGGCTTGCACCGTTGGACGGTCTACCAGTTTGGTGACGCCACAACGATAATCCAGCAGATGCTGTGCCTCCTGCGGCTTCAACTGACGGTCGGTAAACCCGGCCTGATATATCACCTTCCAGAAG  >  NC_000913/3212829‑3213007

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 28 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`