BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,218,562	G→A	V109V (GTC→GTT)	ygjH ←	putative tRNA synthetase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,218,562	0	G	A	97.1%	699.3 / 2.1	206	V109V (GTC→GTT)	ygjH	putative tRNA synthetase
Reads supporting (aligned to +/- strand): ref base G (4/2); new base A (116/84); total (120/86)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.67e-01

Only 100 of 222 total aligned reads displayed.

TAAGGCGCTGGCGGCCATGCGAGTAAGTGTCGAAGAAGCGTAATATCAAATCGGATGGCGATACGACGTCGCCATCCGATCTTTTTATACGACGCGTACGCCCGCAGGCATCATCCGCTCCGGCGTTAACAGCACGCTTTCACTGCCGTCATCGGTTTCCGCACACAACAGCATGCATTC  >  NC_000913/3218472‑3218651

   ggCGCTGGCGGCCATGCGAGTAAGTGTCGAAGAAGCGTAATATCAAATCGGATGGCGATACGACGTCGCCATCCGATCTTTTTATACAAcgcg                                                                                      <  1:465945/93‑1 (MQ=255)

    gcgcTGGCGGCCATGCGAGTAAGTGTCGAAGAAGCGTAATATCAAATCGGATGGCGATACGACGTCGCCATCCGATCTTTTTATACAACGCGt                                                                                     <  2:4131654/93‑1 (MQ=255)

     ggcTGGCGGCCATGCGAGTAAGTGTCGAAGAAGCGTAATATCAAATCGGATGGCGATACGACGTCGCCATCCGATCTTTTTATACAACGCGTa                                                                                    <  1:1063573/92‑1 (MQ=255)

         ggcggcCATGCGAGTAAGTGTCGAAGAAGCGTAATATCAAATCGGATGGCGATACGACGTCGCCATCCGATCTTTTTATACAACGCGTACGcc                                                                                >  1:2137189/1‑93 (MQ=255)

             gcCATGCGAGTAAGTGTCGAAGAAGCGTAATATCAAATCGGATGGCGATACGACGTCGCCATCCGATCTTTTTATACAACGCGTACGCCCGCa                                                                            >  2:3883650/1‑93 (MQ=255)

             gcCATGCGAGTAAGTGTCGAAGAAGCGTAATATCAAATCGGATGGCGATACGACGTCGCCATCCGATCTTTTTATACAACGCGTACGCCCGCa                                                                            >  1:823816/1‑93 (MQ=255)

              ccATGCGAGTAAGTGTCGAAGAAGCGTAATATCAAATCGGATGGCGATACGACGTCGCCATCCGATCTTTTTATACAACGCGTACGCCCGCAg                                                                           <  2:4481103/93‑1 (MQ=255)

                    gAGTAAGTGTCGAAGAAGCGTAATATCAAATCGGATGGCGATACGACGTCGCCATCCGATCTTTTTATACAACGCGTACGCCCGCAGGcatca                                                                     >  2:2094668/1‑93 (MQ=255)

                    gAGTAAGTGTCGAAGAAGCGTAATATCAAATCGGATGGCGATACGACGTCGCCATCCGATCTTTTTATACAACGCGTACGCCCGCAGGcatca                                                                     >  2:839509/1‑93 (MQ=255)

                      gTAAGTGTCGAAGAAGCGTAATATCAAATCGGATGGCGATACGACGTCGCCATCCGATCTTTTTATACAACGCGTACGCCCGCAGGCatcatc                                                                   >  2:3486414/1‑93 (MQ=255)

                      gTAAGTGTCGAAGAAGCGTAATATCAAATCGGATGGCGATACGACGTCGCCATCCGATCTTTTTATACAACGCGTACGCCCGCAGGCatcatc                                                                   >  1:3751177/1‑93 (MQ=255)

                      gTAAGTGTCGAAGAAGCGTAATATCAAATCGGATGGCGATACGACGTCGCCATCCCATCTTTTTTTACAACGCGTACGCCCCCAGGCatcatc                                                                   >  1:2402847/1‑93 (MQ=255)

                       tAAGTGTCGAAGAAGCGTAATATCAAATCGGATGGCGATACGACGTCGCCATCCGATCTTTTTATACAACGCGTACGCCCGCGGGCATCATcc                                                                  >  2:2649196/1‑93 (MQ=255)

                       tAAGTGTCGAAGAAGCGTAATATCAAATCGGATGGCGATACGACGTCGCCATCCGATCTTTTTATACAACGCGTACGCCCGCAGGCATCATcc                                                                  <  2:1032843/93‑1 (MQ=255)

                         aGTGTCGACGAAGCGTAATATCAAATCGGATGGCGATACGACGTCGCCATCCGATCTTTTTATACAACGCGTACGCCCGCAGGCATCATCCGc                                                                >  2:4570649/1‑93 (MQ=255)

                         aGTGTCGAAGAAGCGTAATATCAAATCGGATGGCGATACGACGTCGCCATCCGATCTTTTTATACAACGCGTACGCCCGCAGGCATCATCCGc                                                                <  1:904778/93‑1 (MQ=255)

                             tCGAAGAAGCGTAATATCAAATCGGATGGCGATACGACGTCGCCATCCGATCTTTTTATACAACGCGTACGCCCGCAGGCATCATCCGCTCCg                                                            >  1:4001812/1‑93 (MQ=255)

                               gaagaaGCGTAATATCAAATCGGATGGCGATACGACGTCGCCATCCGATCTTTTTATACAACGCGTACGCGCGCAGGCATCATCCGCTCCggg                                                          >  1:3723937/1‑92 (MQ=255)

                                aagaagCGTAATATCAAATCGGATGGCGATACGACGTCGCCATCCGATCTTTTTATACAACGCGTACGCCCGCAGGCATCATCCGCTCCGGCg                                                         >  1:2775100/1‑93 (MQ=255)

                                 agaagCGTAATATCAAATCGGATGGCGATACGACGTCGCCATCCGATCTTTTTATACAACGCGTACGCCCGCAGGCATTATCCGCCTCGGCGt                                                        >  2:4608485/1‑93 (MQ=255)

                                 agaagCGTAATATCAAATCGGATGGCGATACGACGTCGCCATCCGATCTTTTTATACAACGCGTACGCCCGCAGGCATCATCCGCTCCGGCGt                                                        >  2:4090426/1‑93 (MQ=255)

                                 agaagCGTAATATCAAATCGGATGGCGATACGACGTCGCCATCCGATCTTTTTATACAACGCGTACGCCCGCAGGCATCATCCGCTCCGGCGt                                                        >  1:3728728/1‑93 (MQ=255)

                                  gaagCGTAATATCAAATCGGATGGCGATACGACGTCGCCATCCGATCTTTTTATACAACGCGTACGCCCGCAGGCATCATCCGCTCCGGCGtt                                                       >  2:1711836/1‑93 (MQ=255)

                                    agCGTAATATCAAATCGGATGGCGATACGACGTCGCCATCCGATCTTTTTATACAACGCGTACGCCCGCAGGCATCATCCGCTCCGGCGTTaa                                                     <  2:1390651/93‑1 (MQ=255)

                                    agCGTAATATCAAATCGGATGGCGATACGACGTCGCCATCCGATCTTTTTATACAACGCGTACGCCCGCAGGCATCATCCGCTCCGGCGTTaa                                                     >  1:1016108/1‑93 (MQ=255)

                                     gCGTAATATCAAATCGGATGGCGATACGACGTCGCCATCCGATCTTTTTATACAACGCGTACGCCCGCAGGCATCATCCGCTCCGGCGGTAAc                                                    >  2:94127/1‑93 (MQ=255)

                                      cGTAATATCAAATCGGATGGCGATACGACGTCGCCATCCGATCTTTTTATACAACGCGTACGCCCGCAGGCATCATCCGCTCCGGCGTTAACa                                                   <  2:1974821/93‑1 (MQ=255)

                                      cGTAATATCAAATCGGATGGCGATACGACGTCGCCATCCGATCTTTTTATACAACGCGTACGCCCGCAGGCATCATCCGCTCCGGCGTTAACa                                                   >  2:1521936/1‑93 (MQ=255)

                                       gTAATATCAAATCGGATGGCGATACGACGTCGCCATCCGATCTTTTTATACAACGCGTACGCCCGCAGGCATCATCCGCTCCGGCGTTAACAg                                                  >  2:3709293/1‑93 (MQ=255)

                                        tAATATCAAATCGGATGGCGATACGACGTCGCCATCCGATCTTTTTATACAACGCGTACGCCCGCAGGCATCATCCGCTCCGGCGTTAACAGc                                                 >  2:3622005/1‑93 (MQ=255)

                                        tAATATCAAATCGGATGGCGATACGACGTCGCCATCCGATCTTTTTATACAACGCGTACGCCCGCAGGCATCATCCGCTCCGGCGTTAACAGc                                                 >  1:1848564/1‑93 (MQ=255)

                                        tAATATCAAATCGGATGGCGATACGACGTCGCCATCCGATCTTTTTATACAACGCGTACGCCCGCAGGCATCATCCGCTCCGGCGTTAACAGc                                                 >  1:761082/1‑93 (MQ=255)

                                        tAATATCAAATCGGATGGCGATACGACGTCGCCATCCGATCTTTTTATAAAACGCGTACGCCCGCAGGCATCATCCGCTCCGGCGTTCACCGc                                                 >  1:192109/1‑93 (MQ=255)

                                         aaTATCAAATCGGATGGCGATACGACGTCGCCATCCGATCTTTTTATACAACGCGTACGCCCGCAGGCATCATCCGCTCCGGCGTTAACAGCa                                                <  1:3133360/93‑1 (MQ=255)

                                         aaTATCAAATCGGATGGCGATACGACGTCGCCATCCGATCTTTTTATACAAAGCGTACGCCCGCAGGCATCATACGCTCCGGCGTTTAAAGCa                                                >  1:3232288/1‑93 (MQ=255)

                                          atatCAAATCGGATGGCGATACGACGTCGCCATCCGATCTTTTTATACAACGCGTACGCCCGCAGGCATCATCCGCTCCGGCGTTAACAGCAc                                               >  2:3081833/1‑93 (MQ=255)

                                          atatCAAATCGGATGGCGATACGACGTCGCCATCCGATCTTTTTATACAACGCGTACGCCCGCAGGCATCATCCGCTCCGGCGTTAACAGCAc                                               <  1:3435666/93‑1 (MQ=255)

                                           tatCAAATCGGATGGCGATACGACGTCGCCATCCGATCTTTTTATACAACGCGTACGCCCGCAGGCATCATCCGCTCCGGCGTTAACAGCACg                                              >  2:1677849/1‑93 (MQ=255)

                                            atCAAATCGGATGGCGATACGACGTCGCCATCCGATCTTTTTATACAACGCGTACGCCCGCAGGCATCATCCGCTCCGGCGTTAACAGCACGc                                             >  2:2971614/1‑93 (MQ=255)

                                             tCAAATCGGATGGCGATACGACGTCGCCATCCGATCTTTTTATACAACGCGTACGCCCGCAGGCATCATCCGCTCCGGCGTTAACAGCACGCt                                            >  1:2115147/1‑93 (MQ=255)

                                               aaaGCGGAGGGCTATACGGCGTCGCCATCCGATCTTTTTATACAACGCGTACGCCCGCAGGCATCATCCGCTCCGGCGTTAACAGCACTCttt                                          <  2:3517138/93‑1 (MQ=255)

                                                  acGGATGGCGATACGACGTCGCCATCCGATCTTTTTATACAACGCGTACGCCCGCAGGCATCATCCGCTCCGGCGTTAACAGCACGCTTTCAc                                       <  1:1581683/92‑1 (MQ=255)

                                                   cGGATGTCCATCCGACGTCGCCATCCGATCTTTTTATACAACGCGTACGCCCGCAGGCATCATCCGCTCCGGCGTTAACAGCACGCTTTCACt                                      <  1:2710548/93‑1 (MQ=255)

                                                   cGGATGGCGATACGACGTCGCCATCCGATCTTTTTATACAACGCGTACGCCCGCAGGCATCATCCGCTCCGGCGTTAACAGCACGCTTTCACt                                      <  2:1299796/93‑1 (MQ=255)

                                                     gATGGCGATACGACGTCGCCATCCGATCTTTTTATACAACGCGTACGCCCGCAGGCATCATCCGCTCCGGCGTTAACAGCACGCTTTCACTGc                                    <  1:3073149/93‑1 (MQ=255)

                                                     gATGGCGATACGACGTCGCCATCCGATCTTTTTATACAACGCGTACGCCCGCAGGCATCATCCGCTCCGGCGTTAACAGCACGCTTTCACTGc                                    <  2:921213/93‑1 (MQ=255)

                                                      aTGGCGATACGACGTCGCCATCCGATCTTTTTATACAACGCGTACGCCCGCAGGCATCATCCGCTCCGGCGTTAACAGCACGCTTTCACTGcc                                   >  2:2817182/1‑93 (MQ=255)

                                                       tGGCGATACGACGTCGCCATCCGATCTTTTTATACAACGCGTACGCCCGCAGGCATCATCCGCTCCGGCGTTAACAGCACGCTTTCACTGCCg                                  >  2:2731615/1‑93 (MQ=255)

                                                       tGGCGATACGACGTCGCCATCCGATCTTTTTATACAACGCGTACGCCCGCAGGCATCATCCGCTCCGGCGTTAACAGCACGCTTTCACTGCCg                                  <  1:2954618/93‑1 (MQ=255)

                                                        ggCGATACGACGTCGCCATCCGATCTTTTTATACAACGCGTACGCCCGCAGGCATCATCCGCTCCGGCGTTAACAGCACGCTTTCACTGCCGt                                 >  1:2363562/1‑93 (MQ=255)

                                                          cGATACGACGTCGCCATCCGATCTTTTTATACAACGCGTACGCCCGCAGGCATCATCCGCTCCGGCGTTAACAGCACGCTTTCACTGCCGTCa                               <  1:3306392/93‑1 (MQ=255)

                                                           gATACGACGTCGCCATCCGATCTTTTTATACAACGCGTACGCCCGCAGGCATCATCCGCTCCGGCGTTAACAGCACGCTTTCACTGCCGTCAt                              >  1:3864427/1‑93 (MQ=255)

                                                            aTACGACGTCGCCATCCGATCTTTTTATACAACGCGTACGCCCGCAGGCATCATCCGCTCCGGCGTTAACAGCACGCTTTCACTGCCGTCATc                             >  2:1378361/1‑93 (MQ=255)

                                                             tACGACGTCGCCATCCGATCTTTTTATACAACGCGTACGCCCGCAGGCATCATCCGCTCCGGCGTTAACAGCACGCTTTCACTGCCGTCATCg                            >  2:1850362/1‑93 (MQ=255)

                                                                gacgTCGCCATCCGATCTTTTTATACAACGCGTACGCCCGCAGGCATCATCCGCTCCGGCGTTAACAGCACGCTTTCACTGCCGTCATCGGtt                         >  1:2920281/1‑93 (MQ=255)

                                                                gacgTCGCCATCCGATCTTTTTATACAACGCGTACGCCCGCAGGCATCATCCGCTCCGGCGTTAACAGCACGCTTTCACTGCCGTCATCGGtt                         <  2:3217182/93‑1 (MQ=255)

                                                                 acgTCGCCATCCGATCTTTTTATACAACGCGTACGCCCGCAGGCATCATCCGCTCCGGCGTTAACAGCACGCTTTCACTGCCGTCATCGGttt                        <  1:358356/93‑1 (MQ=255)

                                                                  cgTCGCCATCCGATCTTTTTATACAACGCGTACGCCCGCAGGCATCATCCGCTCCGGCGTTAACAGCACGCTTTCACTGCCGTCATCGGTTTc                       >  2:3304377/1‑93 (MQ=255)

                                                                  cgTCGCCATCCGATCTTTTTATACAACGCGTACGCCCGCAGGCATCATCCGCTCCGGCGTTAACAGCACGCTTTCACTGCCGTCATCGGTTTc                       <  1:188602/93‑1 (MQ=255)

                                                                    tCGCCATCCGATCTTTTTATACAACGCGTACGCCCGCAGGCATCATCCGCTCCGGCGTTAACAGCACGCTTTCACTGCCGTCATCGGTTTCCg                     <  2:4547247/93‑1 (MQ=255)

                                                                     cGCCATCCGATCTTTTTATACAACGCGTACGCCCGCAGGCATCATCCGCTCCGGCGTTAACAGCACGCTTTCACTGCCGTCATCGGTTTCCGc                    <  1:205993/93‑1 (MQ=255)

                                                                      gCCATCCGATCTTTTTATACGACGCGTACGCCCGCAGGCATCATCCGCTCCGGCGTTAACAGCACGCTTTCACTGCCGTCATCGGTTTCCGca                   >  2:1273923/1‑93 (MQ=255)

                                                                      gCCATCCGATCTTTTTATACAACGCGTACGCCCGCAGGCATCATCCGCTCCGGCGTTAACAGCACGCTTTCACTGCCGTCATCGGTTTCCGca                   >  2:3729680/1‑93 (MQ=255)

                                                                       ccATCCGATCTTTTTATACAACGCGTACGCCCGCAGGCATCATCCGCTCCGGCGTTAACAGCACGCTTTCACTGCCGTCATCGGTTTCCGcac                  <  1:1607112/93‑1 (MQ=255)

                                                                       ccATCCGATCTTTTTATACAACGCGTACGCCCGCAGGCATCATCCGCTCCGGCGTTAACAGCACGCTTTCACTGCCGTCATCGGTTTCCGcac                  >  2:2332426/1‑93 (MQ=255)

                                                                         aTCCGATCTTTTTATACAACGCGTACGCCCGCAGGCATCATCCGCTCCGGCGTTAACAGCACGCTTTCACTGCCGTCATCGGTTTCCGcacac                >  2:2214085/1‑93 (MQ=255)

                                                                         aTCCGATCTTTTTATACAACGCGTACGCCCGCAGGCATCATCCGCTCCGGCGTTAACAGCACGCTTTCACTGCCGTCATCGGTTTCCGcacac                >  2:4438739/1‑93 (MQ=255)

                                                                         aTCCGATCTTTTTATACAACGCGTACGCCCGCAGGCATCATCCGCTCCGGCGTTAACAGCACGCTTTCACTGCCGTCATCGGTTTCCGcacac                >  1:1733476/1‑93 (MQ=255)

                                                                         aTCCGATCTTTTTATACAACGCGTACGCCCGCAGGCATCATCCGCTCCGGCGTTAACAGCACGCTTTCACTGCCGTCATCGGTTTCCGcacac                >  1:4110285/1‑93 (MQ=255)

                                                                          tCCGATCTTTTTATACAACGCGTACGCCCGCAGGCATCATCCGCTCCGGCGTTAACAGCACGCTTTCACTGCCGTCATCGGTTTCCGcacaca               <  2:192035/93‑1 (MQ=255)

                                                                            cGATCTTTTTATACAACGCGTACGCCCGCAGGCATCATCCGCTCCGGCGTTAACAGCACGCTTTCACTGCCGTCATCGGTTTCCGCACacaac             >  2:4226847/1‑93 (MQ=255)

                                                                            cGATCTTTTTATACAACGCGTACGCCCGCAGGCATCATCCGCTCCGGCGTTAACAGCACGCTTTCACTGCCGTCATCGGTTTCCGCACacaac             <  2:1789477/93‑1 (MQ=255)

                                                                            cGATCTTTTTATACAACGCGTACGCCCGCAGGCATCATCCGCTCCGGCGTTAACAGCACGCTTTCACTGCCGTCATCGGTTTCCGCACacaac             >  1:3535076/1‑93 (MQ=255)

                                                                            cGATCTTTTTATACAACGCGTACGCCCGCAGGCATCATCCGCTCCGGCGTTAACAGCACGCTTTCACTGCCGTCATCGGTTTCCGCACacaac             <  1:2974290/93‑1 (MQ=255)

                                                                             gATCTTTTTATACAACGCGTACGCCCGCAGGCATCATCCGCTCCGGCGTTAACAGCACGCTTTCACTGCCGTCATCGGTTTCCGCACacaaca            >  2:747332/1‑93 (MQ=255)

                                                                             gATCTTTTTATACAACGCGTACGCCCGCAGGCATCATCCGCTCCGGCGTTAACAGCACGCTTTCACTGCCGTCATCGGTTTCCGCACacaaca            >  1:4581703/1‑93 (MQ=255)

                                                                             gATCTTTTTATACAACGCGTACGCCCGCAGGCATCATCCGCTCCGGCGTTAACAGCACGCTTTCACTGCCGTCATCGGTTTCCGCACacaaca            >  1:4440820/1‑93 (MQ=255)

                                                                             caTCTTTTTATCCAACGCGTCCGCCCGCAGGCATCATCCGCTCCGGCGTTAACGGCACCCTTCCACTGCCGTCATCGGTTTCCGCACacaaca            <  2:3693381/92‑1 (MQ=255)

                                                                              aTCTTTTTATACGACGCGTACGCCCGCAGGCATCATCCGCTCCGGCGTTAACAGCACGCTTTCACTGCCGTCATCGGTTTCCGCACACAACAg           >  1:694579/1‑93 (MQ=255)

                                                                              aTCTTTTTATACAACGCGTACGCCCGCAGGCATCATCCGCTCCGGCGTTAACAGCACGCTTTGACTGCCGTCATCGGTTTCCGCCCACAACAg           >  2:304082/1‑93 (MQ=255)

                                                                              aTCTTTTTATACAACGCGTACGCCCGCAGGCATCATCCGCTCCGGCGTTAACAGCACGCTTTCACTGCCGTCATCGGTTTCCGCACACAACAg           >  2:1690442/1‑93 (MQ=255)

                                                                              aTCTTTTTATACAACGCGTACGCCCGCAGGCATCATCCGCTCCGGCGTTAACAGCACGCTTTCACTGCCGTCATCGGTTTCCGCACACAACAg           >  1:3780059/1‑93 (MQ=255)

                                                                               tCTTTTTATACAACGCGTACGCCCGCAGGCATCATCCGCTCCGGCGTTAACAGCACGCTTTCACTGCCGTCATCGGTTTCCGCACACAACAgc          <  2:166747/93‑1 (MQ=255)

                                                                               tCTTTTTATACAACGCGTACGCCCGCAGGCATCATCCGCTCCGGCGTTAACAGCACGCTTTCACTGCCGTCATCGGTTTCCGCACACAACAgc          >  2:3984386/1‑93 (MQ=255)

                                                                                cTTTTTATACAACGCGTACGCCCGCAGGCATCATCCGCTCCGGCGTTAACAGCACGCTTTCACTGCCGTCATCGGTTTCCGCACACAACAgca         >  1:639903/1‑93 (MQ=255)

                                                                                cTTTTTATACAACGCGTACGCCCGCAGGCATCATCCGCTCCGGCGTTAACAGCACGCTTTCACTGCCGTCATCGGTTTCCGCACACAACAgca         >  2:2612897/1‑93 (MQ=255)

                                                                                 tttttATACAACGCGTACGCCCGCAGGCATCATCCGCTCCGGCGTTAACAGCACGCTTTCACTGCCGTCATCGGTTTCCGCACACAACAgcat        <  1:1179536/93‑1 (MQ=255)

                                                                                 tttttATACAACGCGTACGCCCGCAGGCATCATCCGCTCCGGCGTTAACAGCACGCTTTCACTGCCGTCATCGGTTTCCGCACACAACAgcat        <  1:4504945/93‑1 (MQ=255)

                                                                                 tttttATACAACGCGTACGCCCGCAGGCATCATCCGCTCCGGCGTTAACAGCACGCTTTCACTGCCGTCATCGGTTTCCGCACACAACAgcat        <  1:181987/93‑1 (MQ=255)

                                                                                 tttttATACAACGCGTACGCCCGCAGGCATCATCCGCTCCGGCGTTAACAGCACGCTTTCACTGCCGTCATCGGTTTCCGCACACAACAgcat        <  1:2206572/93‑1 (MQ=255)

                                                                                 tttttATACAACGCGTACGCCCGCAGGCATCATCCGCTCCGGCGTTAACAGCACGCTTTCACTGCCGTCATCGGTTTCCGCACACAACAgcat        >  2:1876063/1‑93 (MQ=255)

                                                                                  ttttATACAACGCGTACGCCCGCAGGCATCATCCGCTCCGGCGTTAACAGCACGCTTTCACTGCCGTCATCGGTTTCCGCACACAACAgcatg       <  2:2273175/93‑1 (MQ=255)

                                                                                    ttATACAACGCGTACGCCCGCAGGCATCATCCGCTCCGGCGTTAACAGCACGCTTTCACTGCCGTCATCGGTTTCCGCACACAACAgcatgca     <  2:4174311/93‑1 (MQ=255)

                                                                                    ttATACAACGCGTACGCCCGCAGGCATCATCCGCTCCGGCGTTAACAGCACGCTTTCACTGCCGTCATCGGTTTCCGCACACAACAgcatgca     <  1:1449791/93‑1 (MQ=255)

                                                                                    ttATACAACGCGTACGCCCGCAGGCATCATCCGCTCCGGCGTTAACAGCACGCTTTCACTGCCGTCATCGGTTTCCGCACACAACAgcatgca     >  2:2360668/1‑93 (MQ=255)

                                                                                     tataCAACGCGTACGCCCGCAGGCATCATCCGCTCCGGCGTTAACAGCAGGCTTTCACTGCCGTCATCGGTTTCCGCACACAACAgcatgcat    >  1:1113823/1‑93 (MQ=255)

                                                                                      ataCAACGCGTACGCCCGCAGGCATCATCCGCTCCGGCGTTAACAGCACGCTTTCACTGCCGTCATCGGTTTCCGCACACAACAGCATGCAtt   <  1:2558094/93‑1 (MQ=255)

                                                                                       taCAACGCGTACGCCCGCAGGCATCATCCGCTCCGGCGTTAACAGCACGCTTTCACTGCCGTCATCGGTTTCCGCACACAACAGCATGCATTc  <  1:1241595/93‑1 (MQ=255)

                                                                                       taCAACGCGTACGCCCGCAGGCATCATCCGCTCCGGCGTTAACAGCACGCTTTCACTGCCGTCATCGGTTTCCGCACACAACAGCATGCATTc  <  2:1172610/93‑1 (MQ=255)

                                                                                       taCAACGCGTACGCCCGCAGGCATCATCCGCTCCGGCGTTAACAGCACGCTTTCACTGCCGTCATCGGTTTCCGCACACAACAGCATGCATTc  >  2:2486670/1‑93 (MQ=255)

TAAGGCGCTGGCGGCCATGCGAGTAAGTGTCGAAGAAGCGTAATATCAAATCGGATGGCGATACGACGTCGCCATCCGATCTTTTTATACGACGCGTACGCCCGCAGGCATCATCCGCTCCGGCGTTAACAGCACGCTTTCACTGCCGTCATCGGTTTCCGCACACAACAGCATGCATTC  >  NC_000913/3218472‑3218651

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 18 ≤` `ATCG/ATCG` `< 26 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`