BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,219,876	G→A	G257D (GGC→GAC)	ebgR →	EbgR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,219,876	0	G	A	99.5%	663.8 / NA	189	G257D (GGC→GAC)	ebgR	EbgR
Reads supporting (aligned to +/- strand): ref base G (0/1); new base A (103/85); total (103/86)
Fisher's exact test for biased strand distribution p-value = 4.55e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 198 total aligned reads displayed.

AAATGCTGGCGCGGGAAGACTATCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGGCCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGATATCCCCACCGCGCGATTTACCTTTCCGCCGCTCTCCACCGTGCGCA  >  NC_000913/3219786‑3219965

aaaTGCTGGCGCGGGAAGACTATCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGAcc                                                                                         >  2:3390372/1‑93 (MQ=255)

 aaTGCTGGCGCGGGAAGACTATCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGACCt                                                                                        <  2:3358632/93‑1 (MQ=255)

  aTGCTGGCGCGGGAAGACTATCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGACCTg                                                                                       <  1:2878763/93‑1 (MQ=255)

  aTGCTGGCGCGGGAAGACTATCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGAACTGCGGGCAATTCATGAACGAGACCTg                                                                                       >  1:3146967/1‑93 (MQ=255)

   tGCTGGCGCGGGAAGACTATCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTATTGCGGGCAATTCATGAACGAGACGTGa                                                                                      >  1:237200/1‑93 (MQ=255)

   tGCTGGCGCGGGAAGACTATCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGa                                                                                      >  1:3371106/1‑93 (MQ=255)

    gCTGGCGCGGGAAGACTATCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGaa                                                                                     <  2:767332/93‑1 (MQ=255)

         cgcgGGAAGACTATCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATcc                                                                                <  2:2634286/93‑1 (MQ=255)

          gcgGGAAGACTATCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATccc                                                                               >  1:26486/1‑93 (MQ=255)

            gggAAGACTATCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCcac                                                                             >  1:1750397/1‑93 (MQ=255)

              gAAGACTATCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAg                                                                           >  2:2871543/1‑93 (MQ=255)

                aGACTATCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGa                                                                         <  1:3056777/93‑1 (MQ=255)

                 gACTATCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGat                                                                        >  1:4274854/1‑93 (MQ=255)

                 gACTATCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGat                                                                        <  1:1876781/93‑1 (MQ=255)

                 gACTATCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGat                                                                        >  2:4116895/1‑93 (MQ=255)

                 gACTATCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGat                                                                        <  2:418558/93‑1 (MQ=255)

                   cTATCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGatat                                                                      <  2:2707367/93‑1 (MQ=255)

                    tATCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATAtt                                                                     >  2:185414/1‑93 (MQ=255)

                    tATCCGAAGGCACTGTTTGTTGCTTCCGATGCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATAtt                                                                     <  2:1060646/93‑1 (MQ=255)

                     aTCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATAttt                                                                    >  2:2998402/1‑93 (MQ=255)

                      tCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTc                                                                   >  1:2255994/1‑93 (MQ=255)

                      tCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTc                                                                   <  2:2371925/93‑1 (MQ=255)

                        cGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTCGc                                                                 >  1:1015295/1‑93 (MQ=255)

                         gAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTAGCt                                                                >  1:1598450/1‑93 (MQ=255)

                          aaGGCACTGTTTGTTGCTTCCGCTTCCATTGCTATCGGCGTACTGCGGGCAATTCATGCACGAGACCTGAACATCCCACAGGATATTTCGCtt                                                               >  2:2515025/1‑93 (MQ=255)

                          aaGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTCGCtt                                                               <  1:150390/93‑1 (MQ=255)

                          aaGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTCGCtt                                                               <  2:1242049/93‑1 (MQ=255)

                            ggCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTCGTTTAt                                                             <  2:1795750/93‑1 (MQ=255)

                            ggCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTCGCTTAt                                                             <  1:2925353/93‑1 (MQ=255)

                             gCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTCGCTTATc                                                            <  1:72250/93‑1 (MQ=255)

                              cACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTCGCTTATCa                                                           <  1:4469771/93‑1 (MQ=255)

                               ccTGTTTGTTGCGTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTCGCTTATCAg                                                          <  1:4404977/92‑1 (MQ=255)

                               aCTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTCGCTTATCAg                                                          <  2:3214046/93‑1 (MQ=255)

                               aCTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTCGCTTATCAg                                                          <  1:22925/93‑1 (MQ=255)

                               aCTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTCGCTTATCAg                                                          <  1:1001835/93‑1 (MQ=255)

                                cTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTCGCTTATCAGc                                                         >  1:677280/1‑93 (MQ=255)

                                 tgtttgttGCTTCCGATTCTATTGCTATCGGCGTACTGCGGGCAATTCATGACCGAGACCTGAACATCCCACAGGTTATTTCGCTTATCAGCg                                                        >  2:4574664/1‑93 (MQ=255)

                                 tgtttgttGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTCGCTTATCAGCg                                                        >  2:88415/1‑93 (MQ=255)

                                 tgtttgttGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTCGCTTATCAGCg                                                        >  2:594590/1‑93 (MQ=255)

                                 tgtttgttGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTCGCTTATCAGCg                                                        >  2:3490927/1‑93 (MQ=255)

                                  gtttgttGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTCGCTTATCAGCGt                                                       >  2:3089297/1‑93 (MQ=255)

                                   tttgttGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACGTCCCACAGGATATTTCGCTTATCAGCGtt                                                      >  2:2222081/1‑93 (MQ=255)

                                     tgttgCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTaa                                                    <  2:4677614/93‑1 (MQ=255)

                                     tgttgCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTaa                                                    <  1:1342862/93‑1 (MQ=255)

                                      gttgCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAAc                                                   >  2:1186527/1‑93 (MQ=255)

                                      gttgCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAAc                                                   >  1:3314577/1‑93 (MQ=255)

                                      gttgCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAAc                                                   >  1:566756/1‑93 (MQ=255)

                                      gttgCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAAc                                                   >  1:499005/1‑93 (MQ=255)

                                      gttgCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAAc                                                   >  2:4507779/1‑93 (MQ=255)

                                       ttgCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACg                                                  >  1:3844564/1‑93 (MQ=255)

                                        tgCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGa                                                 <  1:4232537/93‑1 (MQ=255)

                                         gCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGat                                                <  2:148886/93‑1 (MQ=255)

                                          cTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGata                                               <  1:3507070/93‑1 (MQ=255)

                                          cTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGata                                               >  1:899847/1‑93 (MQ=255)

                                           ttCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGatat                                              >  2:3887275/1‑93 (MQ=255)

                                           ttCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGatat                                              <  1:3261489/93‑1 (MQ=255)

                                             ccGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGATATcc                                            <  1:1982081/93‑1 (MQ=255)

                                              cGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGATATccc                                           <  2:226464/93‑1 (MQ=255)

                                                aTTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGATATCCCCa                                         >  1:482548/1‑93 (MQ=255)

                                                aTTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGATATCCCCa                                         <  1:3532962/93‑1 (MQ=255)

                                                 ttCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGATATCCCCAc                                        >  2:2886855/1‑93 (MQ=255)

                                                 ttCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGATATCCCCAc                                        >  1:2446778/1‑93 (MQ=255)

                                                  tCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGATATCCCCAcc                                       >  1:3570581/1‑93 (MQ=255)

                                                    cATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGATATCCCCACcgc                                     >  1:2581224/1‑93 (MQ=255)

                                                     aTTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGATATCCCCACcgcg                                    >  2:103116/1‑93 (MQ=255)

                                                     aTTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGATATCCCCACcgcg                                    >  1:246744/1‑93 (MQ=255)

                                                       tGCTATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGATATCCCCACcgcgcg                                  <  2:4360668/93‑1 (MQ=255)

                                                        gCTATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGATATCCCCACCGCGCGa                                 <  2:3524973/93‑1 (MQ=255)

                                                         cTATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGATATCCCCACCGCGCGAt                                >  1:4684980/1‑93 (MQ=255)

                                                          tATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGATATCCCCACCGCGCGAtt                               <  2:3688923/93‑1 (MQ=255)

                                                          tATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGATATCCCCACCGCGCGAtt                               >  1:1819049/1‑93 (MQ=255)

                                                          tATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGATATCCCCACCGCGCGAtt                               >  1:2800212/1‑93 (MQ=255)

                                                          tATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGATATCCCCACCGCGCGAtt                               >  1:3701767/1‑93 (MQ=255)

                                                          tATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGATATCCCCACCGCGCGAtt                               >  2:564433/1‑93 (MQ=255)

                                                          tATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGATATCCCCACCGCGCGAtt                               >  2:131169/1‑93 (MQ=255)

                                                          tATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGATATCCCCACCGCGCGAtt                               >  1:4056226/1‑93 (MQ=255)

                                                           aTCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGATATCCCCACCGCGCGAttt                              >  2:2789250/1‑93 (MQ=255)

                                                            tCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGATATCCCCACTGCGCGATTTa                             >  2:2862122/1‑93 (MQ=255)

                                                              ggCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGATATCCCCACCGCGCGATTTAcc                           >  1:3189263/1‑93 (MQ=255)

                                                              ggCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGATATCCCCACCGCGCGATTTAcc                           <  1:621671/93‑1 (MQ=255)

                                                              ggCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGATATCCCCACCGCGCGATTTAcc                           <  2:1180617/93‑1 (MQ=255)

                                                              ggCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGATATCCCCACCGCGCGATTTAcc                           >  1:3087442/1‑93 (MQ=255)

                                                                 gTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGATATCCCCACCGCGCGATTTACCttt                        <  2:1444524/93‑1 (MQ=255)

                                                                  tACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGATATCCCCACCGCGCGCTTTACCTTTc                       >  2:1910620/1‑93 (MQ=255)

                                                                  tACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGATATCCCCACCGCGCGATTTACCTTTc                       <  1:3941966/93‑1 (MQ=255)

                                                                  tACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGATATCCCCACCGCGCGATTTACCTTTc                       <  1:1203155/93‑1 (MQ=255)

                                                                    cTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGATATCCCCACCGCGCGATTTACCTTTccg                     >  2:4026280/1‑93 (MQ=255)

                                                                      gCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTCGCTTAACAGCGTTAACGATATCCCCACCGCGCGATTTACCTTTccgcc                   >  1:1671417/1‑93 (MQ=255)

                                                                        gggCATTTCATGACCGCGACCTGACCATCCCACAGGATATTACGCTTATCAGCGTTAACGATATCCCCACCGCGCGATTTACCTTTCcgccgc                 <  2:4687966/93‑1 (MQ=255)

                                                                         ggCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGATATCCCCACCGCGCGATTTACCTTTCCGCCGct                <  1:1700279/93‑1 (MQ=255)

                                                                            aaTTCATGAACGAGACCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGATATCCCCACCGCGCGATTTACCTTTCCGCCGCtctc             >  1:997365/1‑93 (MQ=255)

                                                                            aaTTCATGAACGAGACCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGATATCCCCACCGCGCGATTTACCTTTCCGCCGCtctc             >  1:2600481/1‑93 (MQ=255)

                                                                             aTTCATGAACGAGACCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGATATCCCCACCGCGCGATTTACCTTTCCGCCGCTCTcc            >  1:2152000/1‑93 (MQ=255)

                                                                              ttCATGAACGAGACCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGATATCCCCACCGCGCGATTTACCTTTCCGCCGCTCTCCa           >  1:4114903/1‑93 (MQ=255)

                                                                                cATGAACGAGACCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGATATCCCCACCGCGCGATTTACCTTTCCGCCGCTCTCCAcc         >  1:4605167/1‑93 (MQ=255)

                                                                                 aTGAACGAGACCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGATATCCCCACCGCGCGATTTACCTTTCCGCCGCTCTCCACCg        >  1:1646467/1‑93 (MQ=255)

                                                                                  tGAACGAGACCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGATATCCCCACCGCGCGATTTACCTTTCCGCCGCTCTCCACCGt       >  1:807440/1‑93 (MQ=255)

                                                                                  tGAACGAGACCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGATATCCCCACCGCGCGATTTACCTTTCCGCCGCTCTCCACCGt       >  2:3610462/1‑93 (MQ=255)

                                                                                    aaCGAGACCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGATATCCCCACCGCGCGATTTACCTTTCCGCCGCTCTCCACCGTgc     <  2:1915666/93‑1 (MQ=255)

                                                                                       gagaCCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGATATCCCCACCGCGCGATTTACCTTTCCGCCGCTCTCCACCGTGCGCa  >  2:3806736/1‑93 (MQ=255)

AAATGCTGGCGCGGGAAGACTATCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGGCCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGATATCCCCACCGCGCGATTTACCTTTCCGCCGCTCTCCACCGTGCGCA  >  NC_000913/3219786‑3219965

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 19 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`