BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,223,631	A→G	E90G (GAA→GGA)	ebgC →	evolved beta‑D‑galactosidase beta subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,223,631	0	A	G	100.0%	737.1 / NA	230	E90G (GAA→GGA)	ebgC	evolved beta‑D‑galactosidase beta subunit
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (99/131); total (99/131)

Only 100 of 234 total aligned reads displayed.

CCGGGCATCGACGCTATTTTGAAGTGCATTACTACCTGCAAGGGCAGCAAAAAATTGAATATGCGCCGAAAGAGACATTACAGGTAGTGGAATATTATCGTGATGAAACTGACCGTGAATATTTAAAAGGCTGCGGAGAAACCGTTGAGGTCCACGAAGGGCAAATCGTTATTTGCGATA  >  NC_000913/3223541‑3223720

ccGGGCATCGACGCTATTTTGAAGTGCATTACTACCTGCAAGGGCAGCAAAAAATTGAATATGCGCCGAAAGAGACATTACAGGTAGTGGGat                                                                                         <  2:3205504/93‑1 (MQ=255)

  gggCATCGACGCTATTTTGAATTGCATTACTACCTGCAAGGGCAGCAAAAAATTGAATATGCGCCGAAAGAGACATTACAGGGAGGGGGatat                                                                                       >  1:127062/1‑93 (MQ=255)

   ggCATCGACGCTATTTTGAAGTGCATTACTACCTGCAAGGGCAGCAAAAAATTGAATATGCGCCGAAAGAGACATTACAGGTAGTGGGAtatt                                                                                      <  1:4616801/93‑1 (MQ=255)

    gCATCGACGCTATTTTGAAGTGCATTACTACCTGCAAGGGCAGCAAAAAATTGAATATGCGCCGAAAGAGACATTACAGGTAGTGGGAtatta                                                                                     <  1:3867340/93‑1 (MQ=255)

    gCATCGACGCTATTTTGAAGTGCATTACTACCTGCAAGGGCAGCAAAAAATTGAATATGCGCCGAAAGAGACATTACAGGTAGTGGGAtatta                                                                                     >  1:4488385/1‑93 (MQ=255)

     cATCGACGCTATTTTGAAGTGCATTACTACCTGCAAGGGCAGCAAAAAATTGAATATGCGCCGAAAGAGACATTACAGGTAGTGGGAtattat                                                                                    <  2:795629/93‑1 (MQ=255)

     cATCGACGCTATTTTGAAGTGCATTACTACCTGCAAGGGCAGCAAAAAATTGAATATGCGCCGAAAGAGACATTACAGGTAGTGGGAtattat                                                                                    <  1:1329540/93‑1 (MQ=255)

      aTCGACGCTATTTTGAAGTGCATTACTACCTGCAAGGGCAGCAAAAAATTGAATATGCGCCGAAAGAGACATTACAGGTAGTGGGATATTATc                                                                                   <  2:4321981/93‑1 (MQ=255)

         gACGCTATTTTGAAGTGCATTACTACCTGCAAGGGCAGCAAAAAATTGAATATGCGCCGAAAGAGACATTACAGGTAGTGGGATATTATCGtg                                                                                >  1:4216839/1‑93 (MQ=255)

         gACGCTATTTTGAAGTGCATTACTACCTGCAAGGGCAGCAAAAAATTGAATATGCGCCGAAAGAGACATTACAGGTAGTGGGATATTATCGtg                                                                                >  2:2949100/1‑93 (MQ=255)

         gACGCTATTTTGAAGTGCATTACTACCTGCAAGGGCAGCAAAAAATTGAATATGCGCCGAAAGAGACATTACAGGTAGTGGGATAGTATCGtg                                                                                >  1:2478167/1‑93 (MQ=255)

          aCGCTATTTTGAAGTGCATTACTACCTGCAAGGGCAGCAAAAAATTGAATATGCGCCGAAAGAGACATTACAGGTAGTGGGATATTATCGtga                                                                               <  2:2069658/93‑1 (MQ=255)

           cGCTATTTTGAAGTGCATTACTACCTGCAAGGGCAGCAAAAAATTGAATATGCGCCGAAAGAGACATTACAGGTAGTGGGATATTATCGtgat                                                                              <  2:3840011/93‑1 (MQ=255)

           cGCTATTTTGAAGTGCATTACTACCTGCAAGGGCAGCAAAAAATTGAATATGCGCCGAAAGAGACATTACAGGTAGTGGGATATTATCGtgat                                                                              <  1:3650754/93‑1 (MQ=255)

           cGCTATTTTGAAGTGCATTACTACCTGCAAGGGCAGCAAAAAATTGAATATGCGCCGAAAGAGACATTACAGGTAGTGGGATATTATCGtgat                                                                              <  2:2023456/93‑1 (MQ=255)

            gCTATTTTGAAGTGCATTACTACCTGCAAGGGCAGCAAAAAATTGAATATGCGCCGAAAGAGACATTACAGGTAGTGGGATATTATCGtgatg                                                                             >  1:3700419/1‑93 (MQ=255)

             cTATTTTGAAGTGCATTACTACCTGCAAGGGCAGCAAAAAATTGAATATGCGCCGAAAGAGACATTACAGGTAGTGGGATATTATCGtgatga                                                                            <  2:4140033/93‑1 (MQ=255)

             cTATTTTGAAGTGCATTACTACCTGCAAGGGCAGCAAAAAATTGAATATGCGCCGAAAGAGACATTACAGGTAGTGGGATATTATCGtgatga                                                                            >  2:569945/1‑93 (MQ=255)

              tATTTTGAAGTGCATTACTACCTGCAAGGGCAGCAAAAAATTGAATATGCGCCGAAAGAGACATTACAGGTAGTGGGATATTATCGTGATGaa                                                                           >  2:2585351/1‑93 (MQ=255)

               aTTTTGAAGTGCATTACTACCTGCAAGGGCAGCAAAAAATTGAATATGCGCCGAAAGAGACATTACAGGTAGTGGGATATTATCGTGATGaaa                                                                          <  2:119908/93‑1 (MQ=255)

               aTTTTGAAGTGCATTACTACCTGCAAGGGCAGCAAAAAATTGAATATGCGCCGAAAGAGACATTACAGGTAGTGGGATATTATCGTGATGaaa                                                                          >  1:1813540/1‑93 (MQ=255)

                ttttGAAGTGCATTACTACCTGCAAGGGCAGCAAAAAATTGAATATGCGCCGAAAGAGACATTACAGGTAGTGGGATATTATCGTGATGAAAc                                                                         <  1:1692459/93‑1 (MQ=255)

                   tGAAGTGCATTACTACCTGCAAGGGCAGCAAAAAATTGAATATGCGCCGAAAGAGACATTACAGGTAGTGGGATATTATCGTGATGAAACTGa                                                                      <  2:2720566/93‑1 (MQ=255)

                   tGAAGTGCATTACTACCTGCAAGGGCAGCAAAAAATTGAATATGCGCCGAAAGAGACATTACAGGTAGTGGGATATTATCGTGATGAAACTGa                                                                      <  1:2864080/93‑1 (MQ=255)

                    gAAGTGCATTACTACCTGCAAGGGCAGCAAAAAATTGAATATGCGCCGAAAGAGACATTACAGGTAGTGGGATATTATCGTGATGAAACTGAc                                                                     <  2:524135/93‑1 (MQ=255)

                    gAAGTGCATTACTACCTGCAAGGGCAGCAAAAAATTGAATATGCGCCGAAAGAGACATTACAGGTAGTGGGATATTATCGTGATGAAACTGAc                                                                     >  1:3362422/1‑93 (MQ=255)

                     aaGTGCATTACTACCTGCAAGGGCAGCAAAAAATTGAATATGCGCCGAAAGAGACATTACAGGTAGTGGGATATTATCGTGATGAAACTGAcc                                                                    >  1:4409988/1‑93 (MQ=255)

                      aGTGCATTACTACCTGCAAGGGCAGCAAAAAATTGAATATGCGCCGAAAGAGACATTACAGGTAGTGGGATATTATCGTGATGAAACTGACCg                                                                   <  1:3500112/93‑1 (MQ=255)

                        tGCATTACTACCTGCAAGGGCAGCAAAAAATTGAATATGCGCCGAAAGAGACATTACAGGTAGTGGGATATTATCGTGATGAAACTGACCGTg                                                                 >  1:3344882/1‑93 (MQ=255)

                        tGCATTACTACCTGCAAGGGCAGCAAAAAATTGAATATGCGCCGAAAGAGACATTACAGGTAGTGGGATATTATCGTGATGAAACTGACCGTg                                                                 >  2:3494466/1‑93 (MQ=255)

                          cATTACTACCTGCAAGGGCAGCAAAAAATTGAATATGCGCCGAAAGAGACATTACAGGTAGTGGGATATTATCGTGATGAAACTGACCGTGaa                                                               <  2:3479472/93‑1 (MQ=255)

                           aTTACTACCTGCAAGGGCAGCAAAAAATTGAATATGCGCCGAAAGAGACATTACAGGTAGTGGGATATTATCGTGATGAAACTGACCGTGAat                                                              >  2:3331811/1‑93 (MQ=255)

                            ttACTACCTGCAAGGGCAGCAAAAAATTGAATATGCGCCGAAAGAGACATTACAGGTAGTGGGATATTATCGTGATGAAACTGACCGTGAata                                                             >  1:3938938/1‑93 (MQ=255)

                            ttACTACCTGCAAGGGCAGCAAAAAATTGAATATGCGCCGAAAGAGACATTACAGGTAGTGGGATATTATCGTGATGAAACTGACCGTGAata                                                             <  2:858192/93‑1 (MQ=255)

                               ctacCTGCAAGGGCAGCAAAAAATTGAATATGCGCCGAAAGAGACATTACAGGTAGTGGGATATTATCGTGATGAAACTGACCGTGAATAttt                                                          <  1:4006973/93‑1 (MQ=255)

                                tacCTGCAAGGGCAGCAAAAAATTGAATATGCGCCGAAAGAGACATTACAGGTAGTGGGATATTATCGTGATGAAACTGACCGTGAATATTTa                                                         >  1:1115/1‑93 (MQ=255)

                                tacCTGCAAGGGCAGCAAAAAATTGAATATGCGCCGAAAGAGACATTACAGGTAGTGGGATATTATCGTGATGAAACTGACCGTGAATATTTa                                                         <  1:2398302/93‑1 (MQ=255)

                                tacCTGCAAGGGCAGCAAAAAATTGAATATGCGCCGAAAGAGACATTACAGGTAGTGGGATATTATCGTGATGAAACTGACCGTGAATATTTa                                                         >  2:2439869/1‑93 (MQ=255)

                                 acCTGCAAGGGCAGCAAAAAATTGAATATGCGCCGAAAGAGACATTACAGGTAGTGGGATATTATCGTGATGAAACTGACCGTGAATATTTaa                                                        <  1:4555706/93‑1 (MQ=255)

                                  ccTGCAAGGGCAGCAAAAAATTGAATATGCGCCGAAAGAGACATTACAGGTAGTGGGATATTATCGTGATGAAACTGACCGTGAATATTTaaa                                                       <  1:156579/93‑1 (MQ=255)

                                   cTGCAAGGGCAGCAAAAAATTGAATATGCGCCGAAAGAGACATTACAGGTAGTGGGATATTATCGTGATGAAACTGACCGTGAATATTTaaaa                                                      >  1:3681045/1‑93 (MQ=255)

                                   cTGCAAGGGCAGCAAAAAATTGAATATGCGCCGAAAGAGACATTACAGGTAGTGGGATATTATCGTGATGAAACTGACCGTGAATATTTaaaa                                                      <  1:1163345/93‑1 (MQ=255)

                                   cTGCAAGGGCAGCAAAAAATTGAATATGCGCCGAAAGAGACATTACAGGTAGTGGGATATTATCGTGATGAAACTGACCGTGAATATTTaaaa                                                      <  1:511974/93‑1 (MQ=255)

                                   cTGCAAGGGCAGCAAAAAATTGAATATGCGCCGAAAGAGACATTACAGGTAGTGGGATATTATCGTGATGAAACTGACCGTGAATATTTaaaa                                                      <  2:2276352/93‑1 (MQ=255)

                                    tGCAAGGGCAGCAAAAAATTGAAGATGCGCCGAAAGAGACATTACAGGTAGTCGAACAATATCGTGATGAAACTGACCATGAATATTTAAAAg                                                     <  2:2942215/93‑1 (MQ=255)

                                     gCAAGGGCAGCAAAAAATTGAATATGCGCCGAAAGAGACATTACAGGTAGTGGGATATTATCGTGATGAAACTGACCGTGAATATTTAAAAgg                                                    >  1:723426/1‑93 (MQ=255)

                                     gCAAGGGCAGCAAAAAATTGAATATGCGCCGAAAGAGACATTACAGGTAGTGGGATATTATCGTGATGAAACTGACCGTGAATATTTAAAAga                                                    >  2:2470450/1‑92 (MQ=255)

                                      cAAGGGCAGCAAAAAATTGAATATGCGCCGAAAGAGACATTACAGGTAGTGGGATATTATCGTGATGAAACTGACCGTGAATATTTAAAAGGc                                                   <  2:2748949/93‑1 (MQ=255)

                                      cAAGGGCAGCAAAAAATTGAATATGCGCCGAAAGAGACATTACAGGTAGTGGGATATTATCGTGATGAAACTGACCGTGAATATTTAAAAGGc                                                   <  1:3096656/93‑1 (MQ=255)

                                       aaGGGCAGCAAAAAATTGAATATGCGCCGAAAGAGACATTACAGGTAGTGGGATATTATCGTGATGAAACTGACCGTGAATATTTAAAAGGCt                                                  <  2:3556312/93‑1 (MQ=255)

                                         gggCAGCAAAAAATTGAATATGCGCCGAAAGAGACATTACAGGTAGTGGGATATTATCGTGATGAAACTGACCGTGAATATTTAAAAGGCTGc                                                <  2:4410912/93‑1 (MQ=255)

                                         gggCAGCAAAAAATTGAATATGCGCCGAAAGAGACATTACAGGTAGTGGGATATTATCGTGATGAAACTGACCGTGAATATTTAAAAGGCTGc                                                <  1:267869/93‑1 (MQ=255)

                                          ggCAGCAAAAAATTGAATATGCGCCGAAAGAGACATTACAGGTAGTGGGATATTATCGTGATGAAACTGACCGTGAATATTTAAAAGGCTGCg                                               <  1:2242097/93‑1 (MQ=255)

                                            cagcaAAAAATTGAATATGCGCCGAAAGAGACATTACAGGTAGTGGGATATTATCGTGATGAAACTGACCGTGAATATTTAAAAGGCTGCGga                                             <  1:1087349/93‑1 (MQ=255)

                                            cagcaAAAAATTGAATATGCGCCGAAAGAGACATTACAGGTAGTGGGATATTATCGTGATGAAACTGACCGTGAATATTTAAAAGGCTGCGga                                             <  2:4483407/93‑1 (MQ=255)

                                            cagcaAAAAATTGAATATGCGCCGAAAGAGACATTACAGGTAGTGGGATATTATCGTGATGAAACTGACCGTGAATATTTAAAAGGCTGCGga                                             <  2:1023948/93‑1 (MQ=255)

                                                aaaaaaTTGAATATGCGCCGAAAGAGACATTACAGGTAGTGGGATATTATCGTGATGAAACTGACCGTGAATATTTAAAAGGCTGCGGAGaaa                                         <  1:815244/93‑1 (MQ=255)

                                                 aaaaaTTGAATATGCGCCGAAAGAGACATTACAGGTAGTGGGATATTATCGTGATGAAACTGACCGTGAATATTTAAAAGGCTGCGGAGAAAc                                        <  2:1256894/93‑1 (MQ=255)

                                                 aaaaaTTGAATATGCGCCGAAAGAGACATTACAGGTAGTGGGATATTATCGTGATGAAACTGACCGTGAATATTTAAAAGGCTGCGGAGAAAc                                        <  2:1701161/93‑1 (MQ=255)

                                                 aaaaaTTGAATATGCGCCGAAAGAGACATTACAGGTAGTGGGATATTATCGTGATGAAACTGACCGTGAATATTTAAAAGGCTGCGGAGAAAc                                        <  1:2183197/93‑1 (MQ=255)

                                                  aaaaTTGAATATGCGCCGAAAGAGACATTACAGGTAGTGGGATATTATCGTGATGAAACTGACCGTGAATATTTAAAAGGCTGCGGAGAAAcc                                       >  2:4089823/1‑93 (MQ=255)

                                                   aaaTTGAATATGCGCCGAAAGAGACATTACAGGTAGTGGGATATTATCGTGATGAAACTGACCGTGAATATTTAAAAGGCTGCGGAGAAACCg                                      <  1:2791465/93‑1 (MQ=255)

                                                    aaTTGAATATGCGCCGAAAGAGACATTACAGGTAGTGGGATATTATCGTGATGAAACTGACCGTGAATATTTAAAAGGCTGCGGAGAAACCGt                                     >  2:4625075/1‑93 (MQ=255)

                                                    aaTTGAATATGCGCCGAAAGAGACATTACAGGTAGTGGGATATTATCGTGATGAAACTGACCGTGAATATTTAAAAGGCTGCGGAGAAACCGt                                     <  1:2581170/93‑1 (MQ=255)

                                                    aaTTGAATATGCGCCGAAAGAGACATTACAGGAAGGGGGATATTATCGTGATGAAACTGACCGTGAATATTTACAAGGCTGCGGAGAAACCGt                                     >  2:1513694/1‑93 (MQ=255)

                                                         aaTATGCGCCGAAAGAGACATTACAGGTAGTGGGATATTATCGTGATGAAACTGACCGTGAATATTTAAAAGGCTGCGGAGAAACCGTTGAgg                                <  1:2094539/93‑1 (MQ=255)

                                                         aaTATGCGCCGAAAGAGACATTACAGGTAGTGGGATATTATCGTGATGAAACTGACCGTGAATATTTAAAAGGCTGCGGAGAAACCGTTGAgg                                >  1:3859215/1‑93 (MQ=255)

                                                          atatGCGCCGAAAGAGACATTACAGGTAGTGGGATATTATCGTGATGAAACTGACCGTGAATATTTAAAAGGCTGCGGAGAAACCGTTGAGGt                               <  2:3420474/93‑1 (MQ=255)

                                                          atatGCGCCGAAAGAGACATTACAGGTAGTGGGATATTATCGTGATGAAACTGACCGTGAATATTTAAAAGGCTGCGGAGAAACCGTTGAGGt                               >  1:4058614/1‑93 (MQ=255)

                                                           tatGTGCAGAAAGAGATATTACAGGTAGTGGGATATTATCGTGATGAACCTGACCGTAAATATTTAAAAGGCTGCGGAGAAACCGTTGAGGTc                              <  2:2923656/93‑1 (MQ=255)

                                                           tatGCGCCGAAAGAGACATTACAGGTAGTGGGATATTATCGTGATGAAACTGACCGTGAATATTTAAAAGGCTGCGGAGAAACCGTTGAGGTc                              >  1:4155943/1‑93 (MQ=255)

                                                              gcgcCGAAAGAGACATTACAGGTAGTGGGATATTATCGTGATGAAACTGACCGTGAATATTTAAAAGGCTGCGGAGAAACCGTTGAGGTCCAc                           <  1:3275353/93‑1 (MQ=255)

                                                                 ccGAAAGAGACATTACAGGTAGTGGGATATTATCGTGATGAAACTGACCGTGAATATTTAAAAGGCTGCGGAGAAACCGTTGAGGTCCACGaa                        <  2:1496515/93‑1 (MQ=255)

                                                                   gAAAGAGACATTACAGGTAGTGGGATATTATCGTGATGAAACTGACCGTGAATATTTAAAAGGCTGCGGAGAAACCGTTGAGGTCCACGAAgg                      <  2:2199452/93‑1 (MQ=255)

                                                                   gAAAGAGACATTACAGGTAGTGGGATATTATCGTGATGAAACTGACCGTGAATATTTAAAAGGCTGCGGAGAAACCGTTGAGGTCCACGAAgg                      >  2:4457572/1‑93 (MQ=255)

                                                                   gAAAGAGACATTACAGGTAGTGGGATATTATCGTGATGAAACTGACCGTGAATATTTAAAAGGCTGCGGAGAAACCGTTGAGGTCCACGAAgg                      <  1:3568025/93‑1 (MQ=255)

                                                                    aaaGAGACATTACAGGTAGTGGGATATTATCGTGATGAAACTGACCGTGAATATTTAAAAGGCTGCGGAGAAACCGTTGAGGTCCACGAAggg                     <  2:717335/93‑1 (MQ=255)

                                                                    aaaGAGACATTACAGGTAGTGGGATATTATCGTGATGAAACTGACCGTGAATATTTAAAAGGCTGCGGAGAAACCGTTGAGGTCCACGAAggg                     <  1:638349/93‑1 (MQ=255)

                                                                    aaaGAGACATTACAGGTAGTGGGATATTATCGTGATGAAACTGACCGTGAATATTTAAAAGGCTGCGGAGAAACCGTTGAGGTCCACGAAggg                     >  2:3048751/1‑93 (MQ=255)

                                                                    aaaGAGACATTACAGGTAGTGGGATATTATCGTGATGAAACTGACCGTGAATATTTAAAAGGCTGCGGAGAAACCGTTGAGGTCCACGAAggg                     >  1:1235501/1‑93 (MQ=255)

                                                                    aaaGAGACATTACAGGTAGTGGGATATTATCGTGATGAAACTGACCGTGAATATTTAAAAGGCTGCGGAGAAACCGTTGAGGTCCACGAAggg                     >  1:1744590/1‑93 (MQ=255)

                                                                    aaaGAGACATTACAGGTAGTGGGATATTATCGTGATGAAACTGACCGTGAATATTTAAAAGGCTGCGGAAAAACCGTTGAGGTCCACGAAggg                     >  2:1847933/1‑93 (MQ=255)

                                                                    aaaGAGACATTACAGGTAGTGGGATATTATCGTGATGAAAATGACCGTGAATATTTAAAAGGATGCGGAGAAACCGTTGAGGTCCACGAAggg                     >  2:232827/1‑93 (MQ=255)

                                                                      agagACATTACAGGTAGTGGGATATTATCGTGATGAAACTGACCGTGAATATTTAAAAGGCTGCGGAGAAACCGTTGAGGTCCACGAAGGGCa                   >  2:282789/1‑93 (MQ=255)

                                                                      agagACATTACAGGTAGTGGGATATTATCGTGATGAAACTGACCGTGAATATTTAAAAGGCTGCGGAGAAACCGTTGAGGTCCACGAAGGGCa                   >  1:927312/1‑93 (MQ=255)

                                                                      agagACATTACAGGTAGTGGGATATTATCGTGATGAAACTGACCGTGAATATTTAAAAGGCTGCGGAGAAACCGTTGAGGTCCACGAAGGGCa                   <  2:1366631/93‑1 (MQ=255)

                                                                        agaCATTACAGGTAGTGGGATATTATCGTGATGAAACTGGCCGTGAATATTTAAAAGGCTGCGGAGAAACCGTTGAGGTCCACGAAGGGCaaa                 <  2:4652426/93‑1 (MQ=255)

                                                                         gaCATTACAGGTAGTGGGATATTATCGTGATGAAACTGACCGTGAACATTTAAAAGGCTGCGGAGAAACCGTTGAGGTCCACGAAGGGCAAAt                <  2:3779916/93‑1 (MQ=255)

                                                                          aCATTACAGGTAGTGGGATATTATCGTGATGAAACTGACCGTGAATATTTAAAAGGCTGCGGAGAAACCGTTGAGGTCCACGAAGGGCAAATc               >  2:1801086/1‑93 (MQ=255)

                                                                          aCATTACAGGTAGTGGGATATTATCGTGATGAAACTGACCGTGAATATTTAAAAGGCTGCGGAGAAACCGTTGAGGTCCACGAAGGGCAAATc               <  1:2217337/93‑1 (MQ=255)

                                                                            aTTACAGGTAGTGGGATATTATCGAGATGAAACTGACCGTGAATATTTAAAAGGCTGCGGAGAAACCGTTGAGGTCCACGAAGGGCAAATCGt             >  1:3391385/1‑93 (MQ=255)

                                                                               aCAGGTAGTGGGATATTATCGTGATGAAACTGACCGTGAATATTTAAAAGGCTGCGGAGAAACCGTTGAGGTCCACGAAGGGCAAATCGTTAt          <  2:1425530/93‑1 (MQ=255)

                                                                                cAGGTAGTGGGATATTATCGTGATGAAACTGACCGTGAATATTTAAAAGGCTGCGGAGAAACCGTTGAGGTCCACGAATGGCAAATCGTTAtt         >  2:2864157/1‑93 (MQ=255)

                                                                                 aGGTAGTGGGATATTATCGTGATGAAACTGACCGTGAATATTTAAAAGGCTGCGGAGAAACCGTTGAGGTCCACGAAGGGCAAATCGTTAttt        <  1:153080/93‑1 (MQ=255)

                                                                                 aGGAAGTGGGATATTAGCGTGATGAAACGGACCGGGAAAATTGAAAAGGCTGCGGAGAAACCGTGGAGGTCCAAGAAGGGCAAATCGTTAttt        <  2:1234667/93‑1 (MQ=255)

                                                                                   gTAGTGGGATATTATCGTGATGAAACTGACCGTGAATATTTAAAAGGCTGCGGAGAAACCGTTGAGGTCCACGAAGGGCAAATCGTTATTTGc      >  2:1994691/1‑93 (MQ=255)

                                                                                     aGTGGGATATTATCGTGATGAAACTGACCGTGAATATTTAAAAGGCTGCGGAGAAACCGTTGAGGTCCACGAAGGGCAAATCGTTATTTGCGa    <  1:2960030/93‑1 (MQ=255)

                                                                                      gTGGGATATTATCGTGATGAAACTGACCGTGAATATTTAAAAGGCTGCGGAGAAACCGTTGAGGTCCACGAAGGGCAAATCGTTATTTGCGat   <  2:4015600/93‑1 (MQ=255)

                                                                                      gTGGGATATTATCGTGATGAAACTGACCGTGAATATTTAAAAGGCTGCGGAGAAACCGTTGAGGTCCACGAAGGGCAAATCGTTATTTGCGat   >  1:1858110/1‑93 (MQ=255)

                                                                                       tGGGATATTATCGTGATGAAACTGACCGTGAATATTTAAAAGGCTGCGGAGAAACCGTTGAGGTCCACGAAGGGCAAATCGTTATTTGCGata  <  2:684816/93‑1 (MQ=255)

CCGGGCATCGACGCTATTTTGAAGTGCATTACTACCTGCAAGGGCAGCAAAAAATTGAATATGCGCCGAAAGAGACATTACAGGTAGTGGAATATTATCGTGATGAAACTGACCGTGAATATTTAAAAGGCTGCGGAGAAACCGTTGAGGTCCACGAAGGGCAAATCGTTATTTGCGATA  >  NC_000913/3223541‑3223720

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 23 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`