BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,228,614	G→A	A696T (GCT→ACT)	ygjK →	putative isomerase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,228,614	0	G	A	99.5%	684.9 / NA	202	A696T (GCT→ACT)	ygjK	putative isomerase
Reads supporting (aligned to +/- strand): ref base G (0/0); major base A (95/106); minor base T (0/1); total (95/107)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01

Only 100 of 211 total aligned reads displayed.

GCCAATGCCGACGCGGTGGTGAAGGTGATGCTCGATCCTAAAGAGTTCAACACCTTTGTCCCGCTGGGAACGGCGGCGTTAACCAATCCGGCTTTTGGCGCTGATATCTACTGGCGTGGGCGCGTATGGGTGGATCAGTTCTGGTTTGGTCTGAAAGGGATGGAGCGTTACGGTTATCGT  >  NC_000913/3228524‑3228703

gCCAATGCCGACGCGGTGGTGAAGGTGATGCTCGATCCTAAAGAGTTCAACACCTTTGTCCCGCTGGGAACGGCGGCGTTAACCAATCCGACt                                                                                         >  1:3431424/1‑93 (MQ=255)

 ccAATGCCGACGCGGTGGTGAAGGTGATGCTCGATCCTAAAGAGTTCAACACCTTTGTCCCGCTGGGAACGGCGGCGTTAACCAATCCGACtt                                                                                        >  1:4253666/1‑93 (MQ=255)

  cAATGCCGACGCGGTGGTGAAGGTGATGCTCGATCCTAAAGAGTTCAACACCTTTGTCCCGCTGGGAACGGCGGCGTTAACCAATCCGACttt                                                                                       <  2:4655466/93‑1 (MQ=255)

  cAATGCCGACGCGGTGGTGAAGGTGATGCTCGATCCTAAAGAGTTCAACACCTTTGTCCCGCTGGGAACGGCGGCGTTAACCAATCCGACttt                                                                                       >  2:2004177/1‑93 (MQ=255)

   aaTGCCGACGCGGTGGTGAAGGTGATGCTCGATCCTAAAGAGTTCAACACCTTTGTCCCGCTGGGAACGGCGGCGTTAACCAATCCGACtttt                                                                                      <  1:4422858/93‑1 (MQ=255)

    aTGCCGACGCGGTGGTGAAGGTGATGCTCGATCCTAAAGAGTTCAACACCTTTGTCCCGCTGGGAACGGCGGCGTTAACCAATCCGACTTTTg                                                                                     >  2:1245770/1‑93 (MQ=255)

    aTGCCGACGCGGTGGTGAAGGTGATGCTCGATCCTAAAGAGTTCAACACCTTTGTCCCGCTGGGAACGGCGGCGTTAACCAATCCGACTTTTg                                                                                     >  2:3254518/1‑93 (MQ=255)

     tGCCGACGCGGTGGTGAAGGTGATGCTCGATCCTAAAGAGTTCAACACCTTTGTCCCGCTGGGAACGGCGGCGTTAACCAATCCGACTTTTgg                                                                                    <  1:1477591/93‑1 (MQ=255)

      gCCGACGCGGTGGTGAAGGTGATGCTCGATCCTAAAGAGTTCAACACCTTTGTCCCGCTGGGAACGGCGGCGTTAACCAATCCGACTTTTGgc                                                                                   <  2:1172887/93‑1 (MQ=255)

       ccGACGCGGTGGTGAAGGTGATGCTCGATCCTAAAGAGTTCAACACCTTTGTCCCGCTGGGAACGGCGGCGTTAACCAATCCGACTTTTGgcg                                                                                  >  2:265768/1‑93 (MQ=255)

        cGACGCGGTGGTGAAGGTGATGCTCGATCCTAAAGAGTTCAACACCTTTGTCCCGCTGGGAACGGCGGCGTTAACCAATCCGACTTTTGgcgc                                                                                 <  1:917590/93‑1 (MQ=255)

         gACGCGGTGGTGAAGGTGATGCTCGATCCTAAAGAGTTCAACACCTTTGTCCCGCTGGGAACGGCGGCGTTAACCAATCCGACTTTTGGCGCt                                                                                >  1:4476640/1‑93 (MQ=255)

         gACGCGGTGGTGAAGGTGATGCTCGATCCTAAAGAGTTCAACACCTTTGTCCCGCTGGGAACGGCGGCGTTAACCAATCCGACTTTTGGCGCt                                                                                <  1:2920929/93‑1 (MQ=255)

          aCGCGGTGGTGAAGGTGATGCTCGATCCTAAAGAGTTCAACACCTTTGTCCCGCTGGGAACGGCGGCGTTAACCAATCCGACTTTTGGCGCTg                                                                               >  1:1448429/1‑93 (MQ=255)

           cgcgGTGGTGAAGGTGATGCTCGATCCTAAAGAGTTCAACACCTTTGTCCCGCTGGGAACGGCGGCGTTAACCAATCCGACTTTTGGCGCTGa                                                                              <  2:4372573/93‑1 (MQ=255)

           cgcgGTGGTGAAGGTGATGCTCGATCCTAAAGAGTTCAACACCTTTGTCCCGCTGGGAACGGCGGCGTTAACCAATCCGACTTTTGGCGCTGa                                                                              <  2:2089085/93‑1 (MQ=255)

           cgcgGTGGTGAAGGTGATGCTCGATCCTAAAGAGTTCAACACCTTTGTCCCGCTGGGAACGGCGGCGTTAACCAATCCGACTTTTGGCGCTGa                                                                              <  1:4020187/93‑1 (MQ=255)

            gcgGTGGTGAAGGTGATGCTCGATCCTAAAGAGTTCAACACCTTTGTCCCGCTGGGAACGGCGGCGTTAACCAATCCGACTTTTGGCGCTGat                                                                             >  1:4186045/1‑93 (MQ=255)

              ggtggtGAAGGTGATGCTCGATCCTAAAGAGTTCAACACCTTTGTCCCGCTGGGAACGGCGGCGTTAACCAATCCGACTTTTGGCGCTGatat                                                                           >  2:1813252/1‑93 (MQ=255)

               gtggtgAAGGTGATGCTCGATCCTAAAGAGTTCAACACCTTTGTCCCGCTGGGAACGGCGGCGTTAACCAATCCGACTTTTGGCGCTGATATc                                                                          <  1:4561885/93‑1 (MQ=255)

                tggtgAAGGTGATGCTCGATCCTAAAGAGTTCAACACCTTTGTCCCGCTGGGAACGGCGGCGTTAACCAATCCGACTTTTGGCGCTGATATCt                                                                         >  2:345747/1‑93 (MQ=255)

                 ggtgAAGGTGATGCTCGATCCTAAAGAGTTCAACACCTTTGTCCCGCTGGGAACGGCGGCGTTAACCAATCCGACTTTTGGCGCTGATATcca                                                                        <  2:2448244/93‑3 (MQ=255)

                 ggtgAAGGTGATGCTCGATCCTAAAGAGTTCAACACCTTTGTCCCGCTGGGAACGGCGGCGTTAACCAATCCGACTTTTGGCGCTGATATCTa                                                                        <  2:2767279/93‑1 (MQ=255)

                 ggtgAAGGTGATGCTCGATCCTAAAGAGTTCAACACCTTTGTCCCGCTGGGAACGGCGGCGTTAACCAATCCGACTTTTGGCGCTGATATCTa                                                                        >  1:1689431/1‑93 (MQ=255)

                   tgAAGGTGATGCTCGATCCTAAAGAGTTCAACACCTTTGTCCCGCTGGGAACGGCGGCGTTAACCAATCCGACTTTTGGCGCTGATATCTACt                                                                      <  2:1741302/93‑1 (MQ=255)

                     aaGGTGATGCTCGATCCTAAAGAGTTCAACACCTTTGTCCCGCTGGGAACGGCGGCGTTAACCAATCCGACTTTTGGCGCTGATATCTACTgg                                                                    <  1:724948/93‑1 (MQ=255)

                     aaGGTGATGCTCGATCCTAAAGAGTTCAACACCTTTGTCCCGCTGGGAACGGCGGCGTTAACCAATCCGACTTTTGGCGCTGATATCTACTgg                                                                    <  1:1138193/93‑1 (MQ=255)

                        gTGATGGTCGATCCTAAAGAGTTCAACACCTTTGTCCCGCTGGGAACGGCGGCGTTAACCAATCCGACTTTTGGCGCTGATATCTACTGGCgg                                                                 >  2:3900454/1‑92 (MQ=255)

                         tGATGCTCGATCCTAAAGAGTTCAACACCTTTGTCCCGCTGGGAACGGCGGCGTTAACCAATCCGACTTTTGGCGCTGATATCTACTGGCGTg                                                                <  2:500831/93‑1 (MQ=255)

                          gATGCTCGATCCTAAAGAGTTCAACACCTTTGTCCCGCTGGGAACGGCGGCGTTAACCAATCCGACTTTTGGCGCTGATATCTACTGGCGTgg                                                               <  1:1641420/93‑1 (MQ=255)

                          gATGCTCGATCCTAAAGAGTTCAACACCTTTGTCCCGCTGGGAACGGCGGCGTTAACCAATCCGACTTTTGGCGCTGATATCTACTGGCGTgg                                                               <  1:55624/93‑1 (MQ=255)

                               tCGATCCTAAAGAGTTGAACACCTTTGTCCCGCTGGGAACGGCGGCGCTAACCAATCCGACTTTTGTCGCTGATATCTACTGGCGTGGGcgcg                                                          >  1:3557480/1‑93 (MQ=255)

                                 gATCCTAAAGAGTTCAACACCTTTGTCCCGCTGGGAACGGCGGCGTTAACCAATCCGACTTTTGGCGCTGATATCTACTGGCGTGGGCGCGTa                                                        >  1:3067403/1‑93 (MQ=255)

                                  aTCCTAAAGAGTTCAACACCTTTGTCCCGCTGGGAACGGCGGCGTTAACCAATCCGACTTTTGGCGCTGATATCTACTGGCGTGGGCGCGTAt                                                       <  2:1695501/93‑1 (MQ=255)

                                  aTCCTAAAGAGTCCACTACCTTTGTCCCGCTGGGAACGGCGGCGTTAACCAATCCGACTTTTGGCGCTGATATCTACTGGCGTGGGCGCGTAt                                                       <  2:3403664/93‑1 (MQ=255)

                                   tCCTAAAGAGTTCAACACCTTTGTCCCGCTGGGAACGGCGGCGTTAACCAATCCGACTTTTTGCGCTGATATCTACTGGCGTGGGCGCGTATg                                                      >  1:3737427/1‑93 (MQ=255)

                                   tCCTAAAGAGTTCAACACCTTTGTCCCGCTGGGAACGGCGGCGTTAACCAATCCGACTTTTGGCGCTGATATCTACTGGCGTGGGCGCGTATg                                                      >  1:2245363/1‑93 (MQ=255)

                                    ccTAAAGAGTTCAACACCTTTGTCCCGCTGGGAACGGCGGCGTTAACCAATCCGACTTTTGGCGCTGATATCTACTGGCGTGGGCGCGTATgg                                                     <  2:2188993/93‑1 (MQ=255)

                                    ccTAAAGAGTTCAACACCTTTGTCCCGCTGGGAACGGCGGCGTTAACCAATCCGACTTTTGGCGCTGATATCTACTGGCGTGGGCGCGTATgg                                                     <  2:3356639/93‑1 (MQ=255)

                                    ccTAAAGAGTTCAACACCTTTGTCCCGCTGGGAACGGCGGCGTTAACCAATCCGACTTTTGGCGCTGATATCTACTGGCGTGGGCGCGTATgg                                                     <  2:1135196/93‑1 (MQ=255)

                                     cTAAAGAGTTCAACACCTTTGTCCCGCTGGGAACGGCGGCGTTAACCAATCCGACTTTTGGCGCTGGTATCTACTGGCGTAGGCGCGGATggg                                                    >  2:3428024/1‑93 (MQ=255)

                                     cTAAAGAGTTCAACACCTTTGTCCCGCTGGGAACGGCGGCGTTAACCAATCCGACTTTTGGCGCTGATATCTACTGGCGTGGGCGCGTATggg                                                    <  1:4535359/93‑1 (MQ=255)

                                      tAAAGAGTTCAACACCTTTGTCCCGCTGGGAACGGCGGCGTTAACCAATCCGACTTTTGGCGCTGATATCTACTGGCGTGGGCGCGTATgggg                                                   >  1:828227/1‑92 (MQ=255)

                                      tAAAGAGTTCAACACCTTTGTCCCGCTGGGAACGGCGGCGTTAACCAATCCGACTTTTGGCGCTGATATCTACTGGCGTGGGCGCGTATgggg                                                   >  1:1283263/1‑92 (MQ=255)

                                      tAAAGAGTTCAACACCTTTGTCCCGCTGGGAACGGCGGCGTTAACCAATCCGACTTTTGGCGCTGATATCTACTGGCGTGGGCGCGTATGGGt                                                   >  2:919723/1‑93 (MQ=255)

                                       aaaGAGTTCAACACCTTTGTCCCGCTGGGAACGGCGGCGTTAACCAATCCGACTTTTGGCGCTGATATCTACTGGCGTGGGCGCGTATGGGTg                                                  <  2:1653969/93‑1 (MQ=255)

                                        aaGAGTTCAACACCTTTGTCCCGCTGGGAACGGCGGCGTTAACCAATCCGACTTTTGGCGCTGATATCTACTGGCGTGGGCGCGTATGGGTgg                                                 >  1:1011838/1‑93 (MQ=255)

                                        aaGAGTTCAACACCTTTGTCCCGCTGGGAACGGCGGCGTTAACCAATCCGACTTTTGGCGCTGATATCTACTGGCGTGGGCGCGTATGGGCgg                                                 >  1:4066969/1‑93 (MQ=255)

                                         agagTTCAACACCTTTGTCCCGCTGGGAACGGCGGCGTTAACCAATCCGACTTTTGGCGCTGATATCTACTGGCGTGGGCGCGTATGGGTGGa                                                >  2:4168422/1‑93 (MQ=255)

                                         agagTTCAACACCTTTGTCACGCTGGGAACGGCGGCGTTAACCAATCCGACTTTTGGCGCTGATATCTACTGGCGTGGGCGCGTATGGGTGGa                                                <  1:2496792/93‑1 (MQ=255)

                                          gagTTCAACACCTTTGTCCCGCTGGGAACGGCGGCGTTAACCAATCCGACTTTTGGCGCTGATATCTACTGGCGTGGGCGCGTATGGGTGGAt                                               <  1:3675685/93‑1 (MQ=255)

                                          gagTTCAACACCTTTGTCCCGCTGGGAACGGCGGCGTTAACCAATCCGACTTTTGGCGCTGATATCTACTGGCGTGGGCGCGTATGGGTGGAt                                               <  2:398680/93‑1 (MQ=255)

                                          gagTTCAACACCTTTGTCCCGCTGGGAACGGCGGCGTTAACCAATCCGACTTTTGGCGCTGATATCTACTGGCGTGGGCGCGTATGGGTGGAt                                               <  2:3172969/93‑1 (MQ=255)

                                            gTTCAACACCTTTGTCCCGCTGGGAACGGCGGCGTTAACCAATCCGACTTTTGGCGCTGATATCTACTGGCGTGGGCGCGTATGGGTGGATCa                                             <  1:167544/93‑1 (MQ=255)

                                                aaCACCTTTGTCCCGCTGGGAACGGCGGCGTTAACCAATCCGACTTTTGGCGCTGATATCTACTGGCGTGGGCGCGTATGGGTGGATCAGTTc                                         >  1:2697447/1‑93 (MQ=255)

                                                aaCACCTTTGTCCCGCTGGGAACGGCGGCGTTAACCAATCCGACTTTTGGCGCTGATATCTACTGGCGTGGGCGCGTATGGGTGGATCAGTTc                                         <  2:4472568/93‑1 (MQ=255)

                                                aaCACCTTTGTCCCGCTGGGAACGGCGGCGTTAACCAATCCGACTTTTGGCGCTGATATCTACTGGCGTGGGCGCGTATGGGTGGATCAGTTc                                         <  1:2001316/93‑1 (MQ=255)

                                                   acCTTTGTCCCGCTGGGAACGGCGGCGTTAACCAATCCGACTTTTGGCGCTGATATCTACTGGCGTGGGCGCGTATGGGTGGATCAGTTCTgg                                      >  2:4010742/1‑93 (MQ=255)

                                                   acCTTTGTCCCGCTGGGAACGGCGGCGTTAACCAATCCGACTTTTGGCGCTGATATCTACTGGCGTGGGCGCGTATGGGTGGATCAGTTCTgg                                      <  2:854579/93‑1 (MQ=255)

                                                   acCTTTGTCCCGCTGGGAACGGCGGCGTTAACCAATCCGACTTTTGGCGCTGATATCTACTGGCGTGGGCGCGTATGGGTGGATCAGTTCTgg                                      <  1:38437/93‑1 (MQ=255)

                                                   acCTTTGTCCCGCTGGGAACGGCGGCGTTAACCAATCCGACTTTTGGCGCTGATATCTACTGGCGTGGGCGCGTATGGGTGGATCAGTTCTgg                                      <  2:4070883/93‑1 (MQ=255)

                                                   acCTTTGTCCCGCTGGGAACGGCGGCGTTAACCAATCCGACTTTTGGCGCTGATATCTACTGGCGTGGGCGCGTATGGGTGGATCAGTTCTgg                                      >  1:2107657/1‑93 (MQ=255)

                                                    ccTTTGTCCCGCTGGGAACGGCGGCGTTAACCAATCCGACTTTTGGCGCTGATATCTACTGGCGTGGGCGCGTATGGGTGGATCAGTTCTGGt                                     <  2:371935/93‑1 (MQ=255)

                                                    ccTTTGTCCCGCTGGGAACGGCGGCGTTAACCAATCCGACTTTTGGCGCTGATATCTACTGGCGTGGGCGCGTATGGGTGGATCAGTTCTGGt                                     >  2:1012463/1‑93 (MQ=255)

                                                     cTTTGTCCCGCTGGGAACGGCGGCGTTAACCAATCCGACTTTTGGCGCTGATATCTACTGGCGTGGGCGCGTATGGGTGGATCAGTTCTGGtt                                    >  1:1091993/1‑93 (MQ=255)

                                                     cTTTGTCCCGCTGGGAACGGCGGCGTTAACCAATCCGACTTTTGGCGCTGATATCTACTGGCGTGGGCGCGTATGGGGGGATCAGTTCTGGtt                                    >  2:2986742/1‑93 (MQ=255)

                                                       ttGTCCCGCTGGGAACGGCGGCGTTAACCAATCCGACTTTTGGCGCTGATATCTACTGGCGTGGGCGCGTATGGGTGGATCAGTTCTGGTTTg                                  <  2:4481595/93‑1 (MQ=255)

                                                        tGTCCCGCTGGGAACGGCGGCGTTAACCAATCCGACTTTTGGCGCTGATATCTACTGGCGTGGGCGCGTATGGGTGGATCAGTTCTGGTTTgg                                 <  1:28053/93‑1 (MQ=255)

                                                         gTCCCGCTGGGAACGGCGGCGTTAACCAATCCGACTTTTGGCGCTGATATCTACTGGCGTGGGCGCGTATGGGTGGATCAGTTCTGGTTTGGt                                >  1:208608/1‑93 (MQ=255)

                                                          tCCCGCTGGGAACGGCGGCGTTAACCAATCCGACTTTTGGCGCTGATATCTACTGGCGTGGGCGCGTATGGGTGGATCAGTTCTGGTTTGGTc                               >  2:57867/1‑93 (MQ=255)

                                                           cccGCTGGGAACGGCGGCGTTAACCAATCCGACTTTTGGCGCTGATATCTACTGGCGTGGGCGCGTATGGGTGGATCAGTTCTGGTTTGGTCt                              <  2:462477/93‑1 (MQ=255)

                                                            ccGCTGGGAACGGCGGCGTTAACCAATCCGACTTTTGGCGCTGATATCTACTGGCGTGGGCGCGTATGGGTGGATCAGTTCTGGTTTGGTCTg                             >  2:1872591/1‑93 (MQ=255)

                                                             cGCTGGGAACGGCGGCGTTAACCAATCCGACTTTTGGCGCTGATATCTACTGGCGTGGGCGCGTATGGGTGGATCAGTTCTGGTTTGGTCTGa                            <  1:2162206/93‑1 (MQ=255)

                                                               cTGGGAACGGCGGCGTTAACCAATCCGACTTTTGGCGCTGATATCTACTGGCGTGGGCGCGTATGGGTGGATCAGTTCTGGTTTGGTCTGaaa                          >  1:2867623/1‑93 (MQ=255)

                                                               cTGGGAACGGCGGCGTTAACCAATCCGACTTTTGGCGCTGATATCTACTGGCGTGGGCGCGTATGGGTGGATCAGTTCTGGTTTGGTCTGaaa                          >  1:583961/1‑93 (MQ=255)

                                                                tGGGAACGGCGGCGTTAACCAATCCGACTTTTGGCGCTGATATCTACTGGCGTGGGCGCGTATGGGTGGATCAGTTCTGGTTTGGTCTGAAAg                         >  1:3332471/1‑93 (MQ=255)

                                                                tGGGAACGGCGGCGTTAACCAATCCGACTTTTGGCGCTGATATCTACTGGCGTGGGCGCGTATGGGTGGATCAGTTCTGGTTTGGTCTGAAAg                         <  2:3222061/93‑1 (MQ=255)

                                                                 gggAACGGCGGCGTTAACCAATCCGACTTTTGGCGCTGATATCTACTGGCGTGGGCGCGTATGGGTGGATCAGTTCTGGTTTGGTCTGAAAgg                        <  2:676157/93‑1 (MQ=255)

                                                                  ggAACGGCGGCGTTAACCAATCCGACTTTTGGCGCTGATATCTACTGGCGTGGGCGCGTATGGGTGGATCAGTTCTGGTTTGGTCTGAAAggg                       <  2:3528754/93‑1 (MQ=255)

                                                                   gAACGGCGGCGTTAACCAATCCGACTTTTGGCGCTGATATCTACTGGCGTGGGCGCGTATGGGTGGATCAGTTCTGGTTTGGTCTGAAAGGGa                      >  1:705956/1‑93 (MQ=255)

                                                                   gAACGGCGGCGTTAACCAATCCGACTTTTGGCGCTGATATCTACTGGCGTGGGCGCGTATGGGTGGATCAGTTCTGGTTTGGTCTGAAAGGGa                      <  2:1567497/93‑1 (MQ=255)

                                                                   gAACGGCGGCGTTAACCAATCCGACTTTTGGCGCTGATATCTACTGGCGTGGGCGCGTATGGGTGGATCAGTTCTGGTTTGGTCTGAAAGGGa                      <  1:3218583/93‑1 (MQ=255)

                                                                     aCGGCGGCGTTAACCAATCCGACTTTTGGCGCTGATATCTACTGGCGTGGGCGCGTATGGGTGGATCAGTTCTGGTTTGGTCTGAAAGGGATg                    >  1:2052580/1‑93 (MQ=255)

                                                                     aCGGCGGCGTTAACCAATCCGACTTTTGGCGCTGATATCTACTGGCGTGGGCGCGTATGGGTGGATCAGTTCTGGTTTGGTCTGAAAGGGATg                    >  2:3037662/1‑93 (MQ=255)

                                                                      cggcggCGTTAACCAATCCGACTTTTGGCGCTGATATCTACTGGCGTGGGCGCGTATGGGTGGATCAGTTCTGGTTTGGTCTGAAAGGGATgg                   <  1:2314581/93‑1 (MQ=255)

                                                                        gcggcgTTAACCAATCCGACTTTTGGCGCTGATATCTACTGGCGTGGGCGCGTATGGGTGGATCAGTTCTGGTTTGGTCTGAAAGGGATGGAg                 >  1:647849/1‑93 (MQ=255)

                                                                            cgTTAACCAATCCGACTTTTGGCGCTGATATCTACTGGCGTGGGCGCGTATGGGTGGATCAGTTCTGGTTTGGTCTGAAAGGGATGGAGCGtt             <  2:1183933/93‑1 (MQ=255)

                                                                             gTTAACCAATCCGACTTTTGGCGCTGATATCTACTGGCGTGGGCGCGTATGGGTGGATCAGTTCTGGTTTGGTCTGAAAGGGATGGAGCGTTa            >  2:141170/1‑93 (MQ=255)

                                                                              ttAACCAATCCGACTTTTGGCGCTGATATCTACTGGCGTGGGCGCGTATGGGTGGATCAGTTCTGGTTTGGTCTGAAAGGGATGGAGCGTTAc           <  1:1350209/93‑1 (MQ=255)

                                                                              ttAACCAATCCGACTTTTGGCGCTGATATCTACTGGCGTGGGCGCGTATGGGTGGATCAGTTCTGGTTTGGTCTGAAAGGGATGGAGCGTTAc           >  1:853471/1‑93 (MQ=255)

                                                                                aaCCAATCCGACTTTTGGCGCTGATATCTACTGGCGTGGGCGCGTATGGGTGGATCAGTTCTGGTTTGGTCTGAAAGGGATGGAGCGTTACgg         <  2:1512973/93‑1 (MQ=255)

                                                                                 aCCAATCCGACTTTTGGCGCTGATATCTACTGGCGTGGGCGCGTATGGGTGGATCAGTTCTGGTTTGGTCTGAAAGGGATGGAGCGTTACGGt        >  2:428336/1‑93 (MQ=255)

                                                                                 aCCAATCCGACTTTTGGCGCTGATATCTACTGGCGTGGGCGCGTATGGGTGGATCAGTTCTGGTTTGGTCTGAAAGGGATGGAGCGTTACGGt        <  1:3386663/93‑1 (MQ=255)

                                                                                   cAATCCGACTTTTGGCGCTGATATCTACTGGCGTGGGCGCGTATGGGTGGATCAGTTCTGGTTTGGTCTGAAAGGGATGGAGCGTTACGGTTa      <  2:3408103/93‑1 (MQ=255)

                                                                                     aTCCGACTTTTGGCGCTGATATCTACTGGCGTGGGCGCGTATGGGTGGATCAGTTCTGGTTTGGTCTGAAAGGGATGGAGCGTTACGGTTATc    <  2:2373046/93‑1 (MQ=255)

                                                                                      tCCGACTTTTGGCGCTGATATCTACTGGCGTGGGCGCGTATGGGTGGATCAGTTCTGGTTTGGTCTGAAAGGGATGGAGCGTTACGGTTATCg   >  1:257912/1‑93 (MQ=255)

                                                                                      tCCGACTTTTGGCGCTGATATCTACTGGCGTGGGCGCGTATGGGTGGATCAGTTCTGGTTTGGTCTGAAAGGGATGGAGCGTTACGGTTATCg   >  2:3313327/1‑93 (MQ=255)

                                                                                       ccGACTTTTGGCGCTGATATCTACTGGCGTGGGCGCGTATGGGTGGATCAGTTCTGGTTTGGTCTGAAAGGGATGGAGCGTTACGGTTATCGt  <  1:2217990/93‑1 (MQ=255)

                                                                                       ccGACTTTTGGCGCTGATATCTACTGGCGTGGGCGCGTATGGGTGGATCAGTTCTGGTTTGGTCTGAAAGGGATGGAGCGTTACGGTTATCGt  <  1:4491616/93‑1 (MQ=255)

                                                                                       ccGACTTTTGGCGCTGATATCTACTGGCGTGGGCGCGTATGGGTGGATCAGTTCTGGTTTGGTCTGAAAGGGATGGAGCGTTACGGTTATCGt  >  1:4641979/1‑93 (MQ=255)

GCCAATGCCGACGCGGTGGTGAAGGTGATGCTCGATCCTAAAGAGTTCAACACCTTTGTCCCGCTGGGAACGGCGGCGTTAACCAATCCGGCTTTTGGCGCTGATATCTACTGGCGTGGGCGCGTATGGGTGGATCAGTTCTGGTTTGGTCTGAAAGGGATGGAGCGTTACGGTTATCGT  >  NC_000913/3228524‑3228703

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 18 ≤` `ATCG/ATCG` `< 26 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`