BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,251,140	G→C	L239V (CTT→GTT)	yhaJ ←	putative transcriptional regulator LYSR‑type

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,251,140	0	G	C	100.0%	288.8 / NA	94	L239V (CTT→GTT)	yhaJ	putative transcriptional regulator LYSR‑type
Reads supporting (aligned to +/- strand): ref base G (0/0); new base C (82/12); total (82/12)

Only 100 of 103 total aligned reads displayed.

TCTCGCTGGTCGATTCCGGGCTGACGACACGCAACCGCCCTTCCGCAATATCTTTTTCGACCATAGGATACGGCATCGTCGCCACGCCAAGCCCCGCCAGTAATGCCTGACGTTTATCTTCAATCGTGCTCACCGTTAAGCGCGGCTGTTTGTCCAGCAGCTGTACGGTCAACACCGG  >  NC_000913/3251050‑3251227

tctcGCTGGTCGATTCCGGGCTGACGACACGCAACCGCCCTTCCGCAATATCTTTTTCGACCATAGGATACGGCATCGTCGCCACGCCAAccc                                                                                       >  2:2060460/1‑93 (MQ=255)

  tcGCTGGTCGATTCCGGGCTGACGACACGCAACCGCCCTTCCGCAATATCTTTTTCGACCATAGGATACGGCATCGTCGCCACGCCAACcccc                                                                                     >  2:3983793/1‑93 (MQ=255)

  tcGCTGGTCGATTCCGGGCTGACGACACGCAACCGCCCTTCCGCAATATCTTTTTCGACCATAGGATACGGCATCGTCGCCACGCCAACcccc                                                                                     <  2:1559576/93‑1 (MQ=255)

   cGCTGGTCGATTCCGGGCTGACGACACGCAACCGCCCTTCCGCAATATCTTTTTCGACCATAGGATACGGCATCGTCGCCACGCCAACCCCCg                                                                                    >  2:4590056/1‑93 (MQ=255)

    gCTGGTCGATTCCGGGCTGACGACACGCAACCGCCCTTCCGCAATATCTTTTTCGACCATAGGATACGGCATCGTCGCCACGCCAACCCCCGc                                                                                   >  1:1246830/1‑93 (MQ=255)

      tGGTCGATTCCGGGCTGACGACACGCAACCGCCCTTCCGCAATATCTTTTTCGACCATAGGATACGGCATCGTCGCCACGCCAACCCCCGCCa                                                                                 >  1:3492971/1‑93 (MQ=255)

      tGGTCGATTCCGGGCTGACGACACGCAACCGCCCTTCCGCAATATCTTTTTCGACCATAGGATACGGCATCGTCGCCACGCCAACCCCCGCCa                                                                                 >  2:4640398/1‑93 (MQ=255)

      tGGTCGATTCCGGGCTGACGACACGCAACCGCCCTTCCGCAATATCTTTTTCGACCATAGGATACGGCATCGTCGCCACGCCAACCCCCGCCa                                                                                 >  1:871400/1‑93 (MQ=255)

        gTCGATTCCGGGCTGACGACACGCAACCGCCCTTCCGCAATATCTTTTTCGACCATAGGATACGGCATCGTCGCCACGCCAACCCCCGCCAGt                                                                               >  1:953792/1‑93 (MQ=255)

           gATTCCGGGCTGACGACACGCAACCGCCCTTCCGCAATATCTTTTTCGACCATAGGATACGGCATCGTCGCCACGCCAACCCCCGCCAGTAAt                                                                            >  1:3081355/1‑93 (MQ=255)

            aTTCCGGGCTGACGACACGCAACCGCCCTTCCGCAATATCTTTTTCGACCATAGGATACGGCATCGTCGCCACGCCAACCCCCGCCAGTAATg                                                                           >  2:1188789/1‑93 (MQ=255)

            aTTCCGGGCTGACGACACGCAACCGCCCTTCCGCAATATCTTTTTCGACCATAGGATACGGCATCGTCGCCACGCCAACCCCCGCCAGTAATg                                                                           >  2:3014356/1‑93 (MQ=255)

            aTTCCGGGCTGACGACACGCAACCGCCCTTCCGCAATATCTTTTTCGACCATAGGATACGGCATCGTCGCCACGCCAACCCCCGCCAGTAATg                                                                           >  1:2418911/1‑93 (MQ=255)

             ttCCGGGCTGACGACACGCAACCGCCCTTCCGCAATATCTTTTTCGACCATAGGATACGGCATCGTCGCCACGCCAACCCCCGCCAGTAATGc                                                                          >  1:4096982/1‑93 (MQ=255)

                  ggCTGACGACACGCAACCGCCCTTCCGCAATATCTTTTTCGACTAAAGGTTACGCCGTCGTCGCCAGCCCAACCCCCCCCCG‑AATGCCTTACg                                                                    >  1:4180201/1‑93 (MQ=255)

                  ggCTGACGACACGCAACCGCCCTTCCGCAATATCTTTTTCGACCATAGGATACGGCATCGTCGCCACGCCAACCCCCGCCAGTAATGCCTGAc                                                                     >  2:4087901/1‑93 (MQ=255)

                  ggCTGACGACACGCAACCGCCCTTCCGCAATATCTTTTTCGACCATAGGATACGGCATCGTCGCCACGCCAACCCCCGCCAGTAATGCCTGAc                                                                     >  1:2394311/1‑93 (MQ=255)

                    cTGACGACACGCAACCGCCCTTCCGCAATATCTTTTTCGACCATAGGATACGGCATCGTCGCCACGCCAACCCCCGCCAGTAATGCCTGACGt                                                                   >  1:4525289/1‑93 (MQ=255)

                    cTGACGACACGCAACCGCCCTTCCGCAATATCTTTTTCGACCATAGGATACGGCATCGTCGCCACGCCAACCCCCGCCAGTAATGCCTGACGt                                                                   >  2:3799043/1‑93 (MQ=255)

                     tGACGACACGCAACCGCCCTTCCGCAATATCTTTTTCGACCATAGGATACGGCATCGTCGCCACGCCAACCCCCGCCAGTAATGCCTGACGtt                                                                  >  2:11506/1‑93 (MQ=255)

                     tGACGACACGCAACCGCCCTTCCGCAATATCTTTTTCGACCATAGGATACGGCATCGTCGCCACGCCAACCCCCGCCAGTAATGCCTGACGtt                                                                  >  1:246408/1‑93 (MQ=255)

                      gacgacACGCAACCGCCCTTCCGCAATATCTTTTTCGACCATAGGATACGGCATCGTCGCCACGCCAACCCCCGCCAGTAATGCCTGACGttt                                                                 >  2:1011539/1‑93 (MQ=255)

                       acgacACGCAACCGCCCTTCCGCAATATCTTTTTCGACCATAGGATACGGCATCGTCGCCACGCCAACCCCCGCCAGTAATGCCTGACGTTTa                                                                >  2:1256682/1‑93 (MQ=255)

                       acgacACGCAACCGCCCTTCCGCAATATCTTTTTCGACCATAGGATACGGCATCGTCGCCACGCCAACCCCCGCCAGTAATGCCTGACGTTTa                                                                <  2:1565702/93‑1 (MQ=255)

                        cgacACGCAACCGCCCTTCCGCAATATCTTTTTCGACCATAGGATACGGCATCGTCGCCACGCCAACCCCCGCCAGTAATGCCTGACGTTTAt                                                               <  2:679270/93‑1 (MQ=255)

                         gacACGCAACCGCCCTTCCGCAATATCTTTTTCGACCATAGGATACGGCATCGTCGCCACGCCAACCCCCGCCAGTAATGCCTGACGTTTATc                                                              >  1:46294/1‑93 (MQ=255)

                          acacGCAACCGCCCTTCCGCAATATCTTTTTCGACCATAGGATACGGCATCGTCGCCACGCCAACCCCCGCCAGTAATGCCTGACGTTTATCt                                                             >  2:2238942/1‑93 (MQ=255)

                          acacGCAACCGCCCTTCCGCAATATCTTTTTCGACCATAGGATACGGCATCGTCGCCACGCCAACCCCCGCCAGTAATGCCTGACGTTTATCt                                                             >  1:4149049/1‑93 (MQ=255)

                           cacGCAACCGCCCTTCCGCAATATCTTTTTCGACCATAGGATACGGCATCGTCGCCACGCCAACCCCCGCCAGTAATGCCTGACGTTTATCtt                                                            >  1:3006909/1‑93 (MQ=255)

                            acGCAACCGCCCTTCCGCAATATCTTTTTCGACCATAGGATACGGCATCGTCGCCACGCCAACCCCCGCCAGTAATGCCTGACGTTTATCTTc                                                           >  2:895098/1‑93 (MQ=255)

                            acGCAACCGCCCTTCCGCAATATCTTTTTCGACCATAGGATACGGCATCGTCGCCACGCCAACCCCCGCCAGTAATGCCTGACGTTTATCTTc                                                           >  1:3879161/1‑93 (MQ=255)

                            acGCAACCGCCCTTCCGCAATATCTTTTTCGACCATAGGATACGGCATCGTCGCCACGCCAACCCCCGCCAGTAATGCCTGACGTTTATCTTc                                                           >  2:800472/1‑93 (MQ=255)

                              gCAACCGCCCTTCCGCAATATCTTTTTCGACCATAGGATACGGCATCGTCGCCACGCCAACCCCCGCCAGTAATGCCTGACGTTTATCTTCaa                                                         >  1:2240467/1‑93 (MQ=255)

                               cAACCGCCCTTCCGCAATATCTTTTTCGACCATAGGATACGGCATCGTCGCCACGCCAACCCCCGCCAGTAATGCCTGACGTTTATCTTCAAt                                                        >  1:4013245/1‑93 (MQ=255)

                                  ccGCCCTTCCGCAATATCTTTTTCGACCATAGGATACGGCATCGTCGCCACGCCAACCCCCGCCAGTAATGCCTGACGTTTATCTTCAATCGt                                                     >  2:752084/1‑93 (MQ=255)

                                  ccGCCCTTCCGCAATATCTTTTTCGACCATAGGATACGGCATCGTCGCCACGCCAACCCCCGCCAGTAATGCCTGACGTTTATCTTCAATCGt                                                     >  1:2029940/1‑93 (MQ=255)

                                    ccccTTCCGCAATATCTTTTTCGACCATAGGATACGGCATCGTCGCCACGCCAACCCCCGCCAGTAATGCCTGACGTTTATCTTCAATCGTGc                                                   <  2:856789/92‑1 (MQ=255)

                                      ccTTCCGCAATATCTTTTTCGACCATAGGATACGGCATCGTCGCCACGCCAACCCCCGCCAGTAATGCCTGACGTTTATCTTCAATCGTGCTc                                                 >  1:4359238/1‑93 (MQ=255)

                                      ccTTCCGCAATATCTTTTTCGACCATAGGATACGGCATCGTCGCCACGCCAACCCCCGCCAGTAATGCCTGACGTTTATCTTCAATCGTGCTc                                                 >  1:4313281/1‑93 (MQ=255)

                                      ccTTCCGCAATATCTTTTTCGACCATAGGATACGGCATCGTCGCCACGCCAACCCCCGCCAGTAATGCCTGACGTTTATCTTCAATCATGCTc                                                 >  2:146671/1‑93 (MQ=255)

                                       cTTCCGCAATATCTTTTTCGACCATAGGATACGGCATCGTCGCCACGCCAACCCCCGCCAGTAATGCCTGACGTTTATCTTCAATCGTGCTcc                                                >  1:4321972/1‑92 (MQ=255)

                                       cTTCCGCAATATCTTTTTCGACCATAGGATACGGCATCGTCGCCACGCCAACCCCCGCCAGTAATGCCTGACGTTTATCTTCAATCGTGCTCa                                                >  1:4260044/1‑93 (MQ=255)

                                       cTTCCGCAATATCTTTTTCGACCATAGGATACGGCATCGTCGCCACGCCAACCCCCGCCAGTAATGCCTGACGTTTATCTTCAATCGTGCTCa                                                <  1:15585/93‑1 (MQ=255)

                                       cTTCCGCAATATCTTTTTCGACCATAGGATACGGCATCGTCGCCACGCCAACCCCCGCCAGTAATGCCTGACGTTTATCTTCAATCGTGCTCa                                                >  1:4538802/1‑93 (MQ=255)

                                       cTTCCGCAATATCTTTTTCGACCATAGGATACGGCATCGTCGCCACGCCAACCCCAGCCAGTAATGCCTGACGTTTATCTTCAATCGTGCTCa                                                >  2:2478762/1‑93 (MQ=255)

                                        ttCCGCAATATCTTTTTGGACCATAGGATACGGCATCGTCGCCACGCCAACCTCCGCCAGTAATGCCTGACGTTTCTCTTCAATCGTGCTCAc                                               >  2:2285802/1‑93 (MQ=255)

                                          ccGCAATATCTTTTTCGACCATAGGATACGGCATCGTCGCCACGCCAACCCCCGCCAGTAATGCCTGACGTTTATCTTCAATCGTGCTCACCg                                             >  1:436134/1‑93 (MQ=255)

                                          ccGCAATATCTTTTTCGACCATAGGATACGGCATCGTCGCCACGCCAACCCCCGCCAGTAATGCCTGACGTTTATCTTCAATCGTGCTCACCg                                             <  2:4524843/93‑1 (MQ=255)

                                            gCAATATCTTTTTCGACCATAGGATACGGCATCGTCGCCACGCCAACCCCCGCCAGTAATGCCTGACGTTTATCTTCAATCGTGCTCACCGtt                                           >  1:3460341/1‑93 (MQ=255)

                                            gCAATATCTTTTTCGACCATAGGATACGGCATCGTCGCCACGCCAACCCCCGCCAGTAATGCCTGACGTTTATCTTCAATCGTGCTCACCGtt                                           >  1:3184072/1‑93 (MQ=255)

                                              aaTATCTTTTTCGACCATAGGATACGGCATCGTCGCCACGCCAACCCCCGCCAGTAATGCCTGACGTTTATCTTCAATCGTGCTCACCGTTaa                                         >  2:368969/1‑93 (MQ=255)

                                              aaTATCTTTTTCGACCATAGGATACGGCATCGTCGCCACGCCAACCCCCGCCAGTAATGCCTGACGTTTATCTTCAATCGTGCTCACCGTTaa                                         <  1:4406310/93‑1 (MQ=255)

                                               atatCTTTTTCGACCATAGGATACGGCATCGTCGCCACGCCAACCCCCGCCAGTAATGCCTGACGTTTATCTTCAATCGTGCTCACCGTTAAg                                        >  2:2737872/1‑93 (MQ=255)

                                               atatCTTTTTCGACCATAGGATACGGCATCGTCGCCACGCCAACCCCCGCCAGTAATGCCTGACGTTTATCTTCAATCGTGCTCACCGTTAAg                                        >  1:3476654/1‑93 (MQ=255)

                                               atatCTTTTTCGACCATAGGATACGGCATCGTCGCCACGCCAACCCCCGCCAGTAATGCCTGACGTTTATCTTCAATCGTGCTCACCGTTAAg                                        >  2:2890316/1‑93 (MQ=255)

                                               atatCTTTTTCGACCATAGGATACGGCATCGTCGCCACGCCAACCCCCGCCAGTAATGCCTGACGATTATCTTCAATCGTGCTCACCGATAAg                                        >  2:1031815/1‑93 (MQ=255)

                                                tatCTTTTTCGACCATAGGATACGGCATCGTCGCCACGCCACCCCCCGCCAGTAATGCCTGACGTTTATCTTCAATCGTGCTCACCGTTAAgc                                       >  1:1040878/1‑93 (MQ=255)

                                                tatCTTTTTCGACCATAGGATACGGCATCGTCGCCACGCCAACCCCCGCCAGTAATGCCTGACGTTTATCTTCAATCGTGCTCACCGTTAAgc                                       >  1:416565/1‑93 (MQ=255)

                                                tatCTTTTTCGACCATAGGATACGGCATCGTCGCCACGCCAACCCCCGCCAGTAATGCCTGACGTTTATCTTCAATCGTGCTCACCGTTAAgc                                       >  1:2917083/1‑93 (MQ=255)

                                                  tCTTTTTCGACCATAGGATACGGCATCGTCGCCACGCCAACCCCCGCCAGTAATGCCTGACGTTTATCTTCAATCGTGCTCACCGTTAAgcgg                                     >  1:290608/1‑92 (MQ=255)

                                                  tCTTTTTCGACCATAGGATACGGCATCGTCGCCACGCCAACCCCCGCCAGTAATGCCTGACGTTTATCTTCAATCGTGCTCACCGTTAAgcgc                                     >  2:3298383/1‑93 (MQ=255)

                                                  tCTTTTTCGACCATAGGATACGGCATCGTCGCCACGCCAACCCCCGCCAGTAATGCCTGACGTTTATCTTCAATCGTGCTCACCGTTAAgcgc                                     >  2:2684627/1‑93 (MQ=255)

                                                   cTTTTTCGACCATAGGATACGGCATCGTCGCCACGCCAACCCCCGCCAGTAATGCCTGACGTTTATCTTCAATCGTGCTCACCGTTAAGcgcg                                    >  2:2864495/1‑93 (MQ=255)

                                                    tttttCGACCATAGGATACGGCATCGTCGCCACGCCAACCCCCGCCAGTAATGCCTGACGTTTATCTTCAATCGTGCTCACCGTTAAGCGCgg                                   >  1:4516158/1‑93 (MQ=255)

                                                    tttttCGACCATAGGATACGGCATCGTCGCCACGCCAACCCCCGCCAGTAATGCCTGACGTTTATCTTCAATCGTGCTCACCGTTAAGCGCgg                                   >  1:3827484/1‑93 (MQ=255)

                                                     ttttCGACCATAGGATACGGCATCGTCGCCACGCCAACCCCCGCCAGTAATGCCTGACGTTTATCTTCAATCGTGCTCACCGTTAAGCGCGGc                                  >  1:1475397/1‑93 (MQ=255)

                                                     ttttCGACCATAGGATACGGCATCGTCGCCACGCCAACCCCCGCCAGTAATGCCTGACGTTTATCTTCAATCGTGCTCACCGTTAAGCGCGGc                                  <  2:3973368/93‑1 (MQ=255)

                                                     ttttCGACCATAGGATACGGCATCGTCGCCACGCCAACCCCCGCCAGTAATGCCTGACGTTTATCTTCAATCGTGCTCACCGTTAACCGCGGc                                  >  1:1475509/1‑93 (MQ=255)

                                                       ttCGACCATAGGATACGGCATCGTCGCCACGCCAACACCCGCCAGTAATGCCTGACGTTTATCTTCCGTCGTGCTCCCCGTTAAGCGCGGCTg                                >  2:3006022/1‑93 (MQ=255)

                                                        tCGACCATAGGATACGGCATCGTCGCCACGCCAACCCCCGCCAGTAATGCCTGACGTTTATCTTCAATCGTGCTCACCGTTAAGCGCGGCTGt                               >  1:521176/1‑93 (MQ=255)

                                                            ccATAGGATACGGCATCGTCGCCACGCCAACCCCCGCCAGTAATGCCTGACGTTTATCTTCAATCGTGCTCACCGTTAAGCGCGGCTGTTTGt                           >  1:3416029/1‑93 (MQ=255)

                                                            ccATAGGATACGGCATCGTCGCCACGCCAACCCCCGCCAGTAATGCCTGACGTTTATCTTCAATCGTGCTCACCGTTAAGCGCGGCTGTTTGt                           >  1:2933496/1‑93 (MQ=255)

                                                             cATAGGATACGGCATCGTCGCCACGCCAACCCCCGCCAGTAATGCCTGACGTTTATCTTCAATCGTGCTCACCGTTAAGCGCGGCTGTTTGTc                          >  2:3701773/1‑93 (MQ=255)

                                                              aTAGGATACGGCATCGTCGCCACGCCAACCCCCGCCAGTAATGCCTGACGTTTATCTTCAATCGTGCTCACCGTTAAGCGCGGCTGTTTGTcc                         >  1:3347972/1‑93 (MQ=255)

                                                               tAGGATACGGCATCGTCGCCACGCCAACCCCCGCCAGTAATGCCTGACGTTTATCTTCAATCGTGCTCACCGTTAAGCGCGGCTGTTTGTCca                        >  1:4398348/1‑93 (MQ=255)

                                                               tAGGATACGGCATCGTCGCCACGCCAACCCCCGCCAGTAATGCCTGACGTTTATCTTCAATCGTGCTCACCGTTAAGCGCGGCTGTTTGTCca                        >  1:3819934/1‑93 (MQ=255)

                                                                aGGATACGGCATCGTCGCCACGCCAACCCCCGCCAGTAATGCCTGACGTTTATCTTCAATCGTGCTCACCGTTAAGCGCGGCTGTTTGTCcag                       >  1:2531868/1‑93 (MQ=255)

                                                                 ggATACGGCATCGTCGCCACGCCAACCCCCGCCAGTAATGCCCGACGTTTATCTTCAATCGTGCTCACCGTTAAGCGCGGCTGTTTTTCcagc                      >  2:2034354/1‑93 (MQ=255)

                                                                  gATACGGCATCGTCGCCACGCCAACCCCCGCCAGTAATGCCTGACGTTTATCTTCAATCGTGCTCACCGTTAAGCGCGGCTGTTTGTCcagca                     >  1:3767184/1‑93 (MQ=255)

                                                                   ggtcGGCGTCGTCTCCACGCCAACCCACGCCAGTAATGCCTGACGTTTATCTTCAATCGTGCTCACCGTTAAGCGCGGCTGTTTGTCcagcag                    <  2:2351110/90‑1 (MQ=255)

                                                                   aTACGGCATCGTCGCCACGCCAACCCCCGCCAGTAATGCCTGACGTTTATCTTCAATCGTGCTCACCGTTAAGCGCGGCTGTTTGTCcagcag                    >  1:289768/1‑93 (MQ=255)

                                                                     aCGGCATCGTCGCCACGCCAACCCCCGCCAGTAATGCCTGACGTTTATCTTCAATCGTGCTCACCGTTAAGCGCGGCTGTTTGTCCAGCAGCt                  >  2:2667176/1‑93 (MQ=255)

                                                                       ggCATCGTCGCCACGCCAACCCCCGCCAGTAATGCCTGACGTTTATCTTCAATCGTGCTCACCGTTAAGCGCGGCTGTTTGTCCAGCAGCTGt                >  2:3728111/1‑93 (MQ=255)

                                                                       ggCATCGTCGCCACGCCAACCCCCGCCAGTAATGCCTGACGTTTATCTTCAATCGTGCTCACCGTTAAGCGCGGCTGTTTGTCCAGCAGCTGt                <  2:2029940/93‑1 (MQ=255)

                                                                          aTCGTCGCCACGCCAACCCCCGCCAGTAATGCCTGACGTTTATCTTCAATCGTGCTCACCGTTAAGCGCGGCTGTTTGTCCAGCAGCTGTACg             >  1:3426350/1‑93 (MQ=255)

                                                                            cgtcgCCACGCCAACCCCCGCCAGTAATGCCTGACGTTTATCTTCAATCGTGCTCACCGTTAAGCGCGGCTGTTTGTCCAGCAGCTGTACGGt           <  2:3121563/93‑1 (MQ=255)

                                                                            cgtcgCCACGCCAACCCCCGCCAGTAATGCCTGACGTTTATCTTCAATCGTGCTCACCGTTAAGCGCGGCTGTTTGTCCAGCAGCTGTACGGt           <  2:1293718/93‑1 (MQ=255)

                                                                            cgtcgCCACGCCAACCCCCGCCAGTAATGCCTGACGTTTATCTTCAATCGTGCTCACCGTTAAGCGCGGCTGTTTGTCCAGCAGCTGTACGGt           <  2:4625302/93‑1 (MQ=255)

                                                                             gtcgCCACGCCAACCCCCGCCAGTAATGCCTGACGTTTATCTTCAATCGTGCTCACCGTTAAGCGCGGCTGTTTGTCCAGCAGCTGTACGGTc          <  1:3681070/93‑1 (MQ=255)

                                                                             gtcgCCACGCCAACCCCCGCCAGTAATGCCTGACGTTTATCTTCAATCGTGCTCACCGTTAAGCGCAGCTGTTTGTCCAGCAGCTGTACGGTc          >  2:3834534/1‑93 (MQ=255)

                                                                              tcgCCACGCCAACCCCCGCCAGTAATGCCTGACGTTTATCTTCAATCGTGCTCACCGTTAAGCGCGGCTGTTTGTCCAGCAGCTGTACGGTCa         >  1:3598131/1‑93 (MQ=255)

                                                                              tcgCCACGCCAACCCCCGCCAGTAATGCCTGACGTTTATCTTCAATCGTGCTCACCGTTAAGCGCGGCTGTTTGTCCAGCAGCTGTACGGTCa         >  1:3807398/1‑93 (MQ=255)

                                                                               cgccacgccaCCCCCCGCCAGTAATGCCTGACGTTTATCTTCAATCGTGCTCACCGTTAAGCGCGGCTGTTTGTCCAGCAGCTGTACGGTCaa        <  2:778192/93‑1 (MQ=255)

                                                                                gccacgccaACCCCCGCCAGTAATGCCTGACGTTTATCTTCAATCGTGCTCACCGTTAAGCGCGGCTGTTTGTCCAGCAGCTGTACGGTCAac       >  2:632650/1‑93 (MQ=255)

                                                                                 ccacgccaACCCCCGCCAGTAATGCCTGACGTTTATCTTCAATCGTGCTCACCGTTAAGCGCGGCTGTTTGTCCAGCAGCTGTACGGTCAaca      >  2:4041444/1‑93 (MQ=255)

                                                                                  cacgccaCCCCCCGCCAGTAATGCCTGACGTTTATCTTCAATCGTGCTCACCGTTAAGCGCGGCTGTTTGTCCAGCAGCTGTACGGTCAacac     <  1:4398791/93‑1 (MQ=255)

                                                                                  cacgccaACCCCCGCCAGTAATGCCTGACGTTTATCTTCAATCATGCTCACCGTTAAGCGCGGCTGTTTGTCCAGCAGCTGTACGGTCAacac     >  1:2734736/1‑93 (MQ=255)

                                                                                    cgccaACCCCCGCCAGTAATGCCTGACGTTTATCTTCAATCGTGCTCACCGTTAAGCGCGGCTGTTTGTCCAGCAGCTGTACGGTCAACACCg   >  1:1165040/1‑93 (MQ=255)

                                                                                    cgccaACCCCCGCCAGTAATGCCTGACGTTTATCTTCAATCGTGCTCACCGTTAAGCGCGGCTGTTTGTCCAGCAGCTGTACAGTCAACACCg   >  2:141299/1‑93 (MQ=255)

                                                                                     gccaACCCCCGCCAGTAATGCCTGACGTTTATCTTCAATCGTGCTCACCGTTAAGCGCGGCTGTTTGTCCAGCAGCTGTACGGTCAACACCgg  >  2:787969/1‑93 (MQ=255)

TCTCGCTGGTCGATTCCGGGCTGACGACACGCAACCGCCCTTCCGCAATATCTTTTTCGACCATAGGATACGGCATCGTCGCCACGCCAAGCCCCGCCAGTAATGCCTGACGTTTATCTTCAATCGTGCTCACCGTTAAGCGCGGCTGTTTGTCCAGCAGCTGTACGGTCAACACCGG  >  NC_000913/3251050‑3251227

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 19 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`