BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,263,328	T→C	V114V (GTA→GTG)	tdcB ←	threonine dehydratase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,263,328	0	T	C	99.6%	749.8 / ‑5.0	237	V114V (GTA→GTG)	tdcB	threonine dehydratase
Reads supporting (aligned to +/- strand): ref base T (0/1); new base C (119/117); total (119/118)
Fisher's exact test for biased strand distribution p-value = 4.98e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.91e-01

Only 100 of 243 total aligned reads displayed.

CATTTCGACAATTTCGCTCACTTTAGCGATAGTGTCGTTGAAGTTATCACCATGCAGAACGACTTCTGCGGAGTAGTCGCACGTTGCCGCTACTTTGGATTTTGGCGCACCTTTTGGCATCACCACTTTACCGTCGATACCCAGCATCGCGCAGGAGAGGGAAACCCCTTGCGCATGGTT  >  NC_000913/3263238‑3263417

cATTTCGACAATTTCGCTCACTTTAGCGATAGTGTCGTTGAAGTTATCACCATGCAGAACGACTTCTGCGGAGTAGTCGCACGTTGCCGCCAc                                                                                         >  1:210816/1‑93 (MQ=255)

cATTTCGACAATTTCGCTCACTTTAGCGATAGTGTCGTTGAAGTTATCACCATGCAGAACGACTTCTGCGGAGTAGTCGCACGTTGCCGCCAc                                                                                         <  2:798067/93‑1 (MQ=255)

  tttCGACAATTTCGCTCACTTTAGCGATAGTGTCGTTGAAGTTATCACCATGCAGAACGACTTCTGCGGAGTAGTCGCACGTTGCCGCCACtt                                                                                       >  2:706717/1‑93 (MQ=255)

  tttCGACAATTTCGCTCACTTTAGCGATAGTGTCGTTGAAGTTATCACCATGCAGAACGACTTCTGCGGAGTAGTCGCACGTTGCCGCCACtt                                                                                       >  1:632874/1‑93 (MQ=255)

   ttCGACAATTTCGCTCACTTTAGCGATAGTGTCGTTGAAGTTATCACCATGCAGAACGACTTCTGCGGAGTAGTCGCACGTTGCCGCCACttt                                                                                      <  2:2571389/93‑1 (MQ=255)

     cGACAATTTCGCTCACTTTAGCGATAGTGTCGTTGAAGTTATCACCATGCAGAACGACTTCTGCGGAGTAGTCGCACGTTGCCGCCACTTTgg                                                                                    <  1:3627823/93‑1 (MQ=255)

      gACAATTTCGCTCACTTTAGCGATAGTGTCGTTGAAGTTATCACCATGCAGAACGACTTCTGCGGAGTAGTCGCACGTTGCCGCCACTTTGGa                                                                                   <  2:3128435/93‑1 (MQ=255)

       aCAATTTCTCTCACTTTAGCGATAGTGTCGTTGAAGTTATCACCATGCAGAACGACTTCTGCGGAGTAGTCGCACGTTGCCGCCACTTTGGAt                                                                                  <  1:4086998/93‑1 (MQ=255)

       aCAATTTCGCTCACTTTAGCGATAGTGTCGTTGAAGTTATCACCATGCAGAACGACTTCTGCGGAGTAGTCGCACGTTGCCGCCACTTTGGAt                                                                                  <  1:367488/93‑1 (MQ=255)

       aCAATTTCGCTCACTTTAGCGATAGTGTCGTTGAAGTTATCACCATGCAGAACGACTTCTGCGGAGTAGTCGCACGTTGCCGCCACTTTGGAt                                                                                  >  1:575778/1‑93 (MQ=255)

         aaTTTCGCTCACTTTAGCGATAGTGTCGTTGAAGTTATCACCATGCAGAACGACTTCTGCGGAGTAGTCGCACGTTGCCGCCACTTTGGAttt                                                                                <  2:227663/93‑1 (MQ=255)

         aaTTTCGCTCACTTTAGCGATAGTGTCGTTGAAGTTATCACCATGCAGAACGACTTCTGCGGAGTAGTCGCACGTTGCCGCCACTTTGGAttt                                                                                <  1:2686900/93‑1 (MQ=255)

          aTTTCGCTCACTTTAGCGATAGTGTCGTTGAAGTTATCACCATGCAGAACGACTTCTGCGGAGTAGTCGCACGTTGCCGCCACTTTGGAtttt                                                                               >  2:4415604/1‑93 (MQ=255)

          aTTTCGCTCACTTTAGCGATAGTGTCGTTGAAGTTATCACCATGCAGAACGACTTCTGCGGAGTAGTCGCACGTTGCCGCCACTTTGGAtttt                                                                               <  1:1996962/93‑1 (MQ=255)

           tttCGCTCACTTTAGCGATAGTGTCGTTGAAGTTATCACCATGCAGAACGACTTCTGCGGAGTAGTCGCACGTTGCCGCCACTTTGGATTTTg                                                                              >  2:3066531/1‑93 (MQ=255)

           tatCGCTCACTTTAGCGATAGTGTCGTTGAAGTTATCACCATGCAGAACGACTTCTGCGGAGTAGTCGCACGTTGCCGCCACTTTGGATTTTg                                                                              >  2:4489565/3‑93 (MQ=255)

               gCTCACTTTAGCGATAGTGTCGTTGAAGTTATCACCATGCAGAACGACTTCTGCGGAGTAGTCGCACGTTGCCGCCACTTTGGATTTTGgcgc                                                                          >  1:100748/1‑93 (MQ=255)

                 tCACTTTAGCGATAGTGTCGTTGAAGTTATCACCATGCAGAACGACTTCTGCGGAGTAGTCGCACGTTGCCGCCACTTTGGATTTTGGCGCAc                                                                        >  2:1004897/1‑93 (MQ=255)

                   ccTTTAGCGATAGTGTCGTTGAAGTTATCACCATGCAGAACGACTTCTGCGGAGTAGTCGCACGTTGCCGCCACTTTGGATTTTGGCGCACCt                                                                      <  1:2233717/92‑1 (MQ=255)

                     tttAGCGATAGTGTCGTTGAAGTTATCACCATGCAGAACGACTTCTGCGGAGTAGTCGCACGTTGCCGCCACTTTGGATTTTGGCGCACCttt                                                                    >  1:3094863/1‑93 (MQ=255)

                     tttAGCGATAGTGTCGTTGAAGTTATCACCATGCAGAACGACTTCTGCGGAGTAGTCGCACGTTGCCGCCACTTTGGATTTTGGCGCACCttt                                                                    >  1:1756971/1‑93 (MQ=255)

                     tttAGCGATAGTGTCGTTGAAGTTATCACCATGCAGAACGACTTCTGCGGAGTAGTCGCACGTTGCCGCCACTTTGGATTTTGGCGCACCttt                                                                    <  1:276093/93‑1 (MQ=255)

                      ttAGCGATAGTGTCGTTGAAGTTATCACCATGCAGAACGACTTCTGCGGAGTAGTCGCACGTTGCCGCCACTTTGGATTTTGGCGCACCtttt                                                                   <  2:2956631/93‑1 (MQ=255)

                      ttAGCGATAGTGTCGTTGAAGTTATCACCATGCAGAACGACTTCTGCGGAGTAGTCGCACGTTGCCGCCACTTTGGATTTTGGCGCACCtttt                                                                   <  1:3328706/93‑1 (MQ=255)

                      ttAGCGATAGTGTCGTTGAAGTTATCACCATGCAGAACGACTTCTGCGGAGGAGTCGCACGTTGCCGCCTCTTTGGATTTTGGCGCACCtttt                                                                   <  2:164830/93‑1 (MQ=255)

                        aGCGATAGTGTCGTTGAAGTTATCACCATGCAGAACGACTTCTGCGGAGTAGTCGCACGTTGCCGCCACTTTGGATTTTGGCGCACCTTTTgg                                                                 <  1:2405122/93‑1 (MQ=255)

                        aGCGATAGTGTCGTTGAAGTTATCACCATGCAGAACGACTTCTGCGGAGTAGTCGCACGTTGCCGCCACTTTGGATTTTGGCGCACCTTTTgg                                                                 >  1:4504274/1‑93 (MQ=255)

                         gCGATAGTGTCGTTGAAGTTATCACCATGCAGAACGACTTCTGCGGAGTAGTTGCACGTTGCCGCCACTTTGGATTTTGGCGCACCTTTTGGc                                                                >  1:2578323/1‑93 (MQ=255)

                         gCGATAGTGTCGTTGAAGTTATCACCATGCAGAACGACTTCTGCGGAGTAGTCGCACGTTGCCGCCACTTTGGATTTTGGCGCACCTTTTGGc                                                                >  2:3364/1‑93 (MQ=255)

                           gATAGTGTCGTTGAAGTTATCACCATGCAGAACGACTTCTGCGGAGTAGTCGCACGTTGCCGCCACTTTGGATTTTGGCGCACCTTTTGGCAt                                                              >  1:3761861/1‑93 (MQ=255)

                            aTAGTGTCGTTGAAGTTATCACCATGCAGAACGACTTCTGCGGAGTAGTCGCACGTTGCCGCCACTTTGGTTTTTGGCGCACCTTTTGGCATc                                                             <  2:3245876/93‑1 (MQ=255)

                             tAGTGTCGTTGAAGTTATCACCATGCAGAACGACTTCTGCGGAGTAGTCGCACGTTGCCGCCACTTTGGATTTTGGCGCACCTTTTGGCATca                                                            >  2:1372602/1‑93 (MQ=255)

                              aGTGTCGTTGAAGTTATCACCATGCAGAACGACTTCTGCGGAGTAGTCGCACGTTGCCGCCACTTTGGATTTTGGCGCACCTTTTGGCATcac                                                           <  2:288786/93‑1 (MQ=255)

                               gtgtCGTTGAAGTTATCACCATGCAGAACGACTTCTGCGGAGTAGTCGCACGTTGCCGCCACTTTGGATTTTGGCGCACCTTTTGGCATcacc                                                          >  1:771809/1‑93 (MQ=255)

                               gtgtCGTTGAAGTTATCACCATGCAGAACGACTTCTGCGGAGTAGTCGCACGTTGCCGCCACTTTGGATTTTGGCGCACCTTTTGGCATcacc                                                          <  1:4324063/93‑1 (MQ=255)

                                tgtCGTTGAAGTTATCACCATGCAGAACGACTTCTGCGGAGTAGTCGCACGTTGCCGCCACTTTGGATTTTGGCGCACCTTTTGGCATcacca                                                         <  1:444576/93‑1 (MQ=255)

                                  tCGTGGAAGTTATCACCATGCAGAACGACTTCTGCGGAGTAGTCGCACGTTGCCGCCACTTTGGATTTTGGCGCACCTTTTGGCATCACCACt                                                       <  2:991166/93‑1 (MQ=255)

                                   cGTTGAAGTTATCACCCTGCAGAACGACTTCTGCGGAGTAGTCGCACGTTGCCGCCGCTTTGGATTTTGGCGCACCTTTTGGCATCACCACtt                                                      >  2:4569692/1‑93 (MQ=255)

                                    gTTGAAGTTATCACCATGCAGAACGACTTCTGCGGAGTAGTCGCACGTTGCCGCTACTTTGGATTTTGGCGCACCTTTTGGCATCACCACttt                                                     <  1:1448501/93‑1 (MQ=255)

                                    gTTGAAGTTATCACCATGCAGAACGACTTCTGCGGAGTAGTCGCACGTTGCCGCCACTTTGGATTTTGGCGCACCTTTTGGCATCACCACttt                                                     <  1:1740116/93‑1 (MQ=255)

                                    gTTGAAGTTATCACCATGCAGAACGACTTCTGCGGAGTAGTCGCACGTTGCCGCCACTTTGGATTTTGGCGCACCTTTTGGCATCACCACttt                                                     <  2:131216/93‑1 (MQ=255)

                                    gTTGAAGTTATCACCATGCAGAACGACTTCTGCGGAGTAGTCGCACGTTGCCGCCACTTTGGATTTTGGCGCACCATTTGGCATCACCACttt                                                     >  1:1281273/1‑93 (MQ=255)

                                     ttGAAGTTATCACCATGCAGAACGACTTCTGCGGAGTAGTCGCACGTTGCCGCCACTTTGGATTTTGGCGCACCTTTTGGCATCACCACTTTa                                                    <  1:1856608/93‑1 (MQ=255)

                                      tGAAGTTATCACCATGCAGAACGACTTCTGCGGAGTAGTCGCACGTTGCCGCCACTTTGGATTTTGGCGCACCTTTTGGCATCACCACTTTAc                                                   >  2:2676647/1‑93 (MQ=255)

                                       gAAGTTATCACCATGCAGAACGACTTCTGCGGAGTAGTCGCACGTTGCCGCCACTTTGGATTTTGGCGCACCTTTTGGCATCACCACTTTAcc                                                  <  1:4554960/93‑1 (MQ=255)

                                       gAAGTTATCACCATGCAGAACGACTTCTGCGGAGTAGTCGCACGTTGCCGCCACTTTGGATTTTGGCGCACCTTTTGGCATCACCACTTTAcc                                                  <  2:2039555/93‑1 (MQ=255)

                                       gAAGTTATCACCATGCAGAACGACTTCTGCGGAGTAGTCGCACGTTGCCGCCACTTTGGATTTTGGCGCACCTTTTGGCATCACCACTTTAcc                                                  <  2:1095500/93‑1 (MQ=255)

                                        aaGTTATCACCATGCAGAACGACTTCTGCGGAGTAGTCGCACGTTGCCGCCACTTTGGATTTTGGCGCACCTTTTGGCATCACCACTTTACCg                                                 <  2:1931033/93‑1 (MQ=255)

                                         aGTTATCACCATGCAGAACGACTTCTGCGGAGTAGTCGCACGTTGCCGCCACTTTGGATTTTGGCGCACCTTTTGGCATCACCACTTTACCGt                                                >  1:2614855/1‑93 (MQ=255)

                                           ttATCACCATGCAGAACGACTTCTGCGGAGTAGTCGCACGTTGCCGCCACTTTGGATTTTGGCGCACCTTTTGGCATCACCACTTTACCGTCg                                              >  1:3150170/1‑93 (MQ=255)

                                            tATCACCATGCAGAACGACTTCTGCGGAGTAGTCGCACGTTGCCGCCACTTTGGATTTTGGCGCACCTTTTGGCATCACCACTTTACCGTCGa                                             <  1:1014065/93‑1 (MQ=255)

                                             aTCACCATGCAGAACGACTTCTGCGGAGTAGTCGCACGTTGCCGCCACTTTGGATTTTGGCGCACCTTTTGGCATCACCACTTTACCGTCGAt                                            >  2:3692726/1‑93 (MQ=255)

                                               cACCATGCAGAACGACTTCTGCGGAGTAGTCGCACGTTGCCGCCACTTTGGATTTTGGCGCACCTTTTGGCATCACCACTTTACCGTCGATAc                                          <  2:1215606/93‑1 (MQ=255)

                                               cACCATGCAGAACGACTTCTGCGGAGTAGTCGCACGTTGCCGCCACTTTGGATTTTGGCGCACCTTTTGGCATCACCACTTTACCGTCGATAc                                          >  2:2975651/1‑93 (MQ=255)

                                                cccATGCAGAACGACTTCTGCGGAGTAGTCGCACGTTGCCGCCACTTTGGATTTTGGCGCACCTTTTGGCATCACCACTTTACCGTCGATAcc                                         <  2:916013/92‑1 (MQ=255)

                                                aCCATGCAGAACGACTTCTGCGGAGTAGTCGCACGTTGCCGCCACTTTGGATTTTGGCGCACCTTTTGGCATCACCACTTTACCGTCGATAcc                                         >  2:725943/1‑93 (MQ=255)

                                                aCCATGCAGAACGACTTCTGCGGAGTAGTCGCACGTTGCCGCCACTTTGGATTTTGGCGCACCTTTTGGCATCACCACTTTACCGTCGATAcc                                         >  2:3098950/1‑93 (MQ=255)

                                                aCCATGCAGAACGACTTCTGCGGAGTAGTCGCACGTTGCCGCCACTTTGGATTTTGGCGCACCTTTTGGCATCACCACTTTACCGTCGATAcc                                         >  1:1621368/1‑93 (MQ=255)

                                                aCCATGCAGAACGACTTCTGCGGAGTAGTCGCACGTTGCCGCCACTTTGGATTTTGGCGCACCTTTTGGCATCACCACTTTACCGTCGATAcc                                         >  2:2933123/1‑93 (MQ=255)

                                                aCCATGCAGAACGACTTCTGCGGAGTAGTCGCACGTTGCCGCCACTTTGGATTTTGGCGCACCTTTTGGCATCACCACTTTACCGTCGATAcc                                         >  2:2318291/1‑93 (MQ=255)

                                                 ccATGCAGAACGACTTCTGCGGAGTAGTCGCACGTTGCCGCCACTTTGGATTTTGGCGCACCTTTTGGCATCACCACTTTACCGTCGATAccc                                        >  1:385244/1‑93 (MQ=255)

                                                  cATGCAGAACTCCTTCTGCGGAGTAGTCGCACGTTGCCGCCACTTTGGATTTTGGCGCACCTTTTGGCATCACCACTTTACCGTCGATACCCa                                       <  1:1561592/93‑1 (MQ=255)

                                                  cATGCAGAACGACTTCTGCGGAGTAGTCGCACGTTGCCGCCACTTTGGATTTTGGCGCACCTTTTGGCATCACCACTTTACCGTCGATACCCa                                       <  2:3546937/93‑1 (MQ=255)

                                                   aTGCAGAACGACTTCTGCGGAGTAGTCGCACGTTGCCGCCACTTTGGATTTTGGCGCACCTTTTGGCATCACCACTTTACCGTCGATACCCAg                                      >  2:4013777/1‑93 (MQ=255)

                                                    tGCAGAACGACTTCTGCGGAGTAGTCGCACGTTGCCGCCACTTTGGATTTTGGCGCACCTTTTGGCATCACCACTTTACCGTCGATACCCAGc                                     >  1:3493427/1‑93 (MQ=255)

                                                     gCAGAACGACTTCTGCGGAGTAGTCGCACGTTGCCGCCACTTTGGATTTTGGCGCACCTTTTGGCATCACCACTTTACCGTCGATACCCAGCa                                    <  2:844622/93‑1 (MQ=255)

                                                      cAGAACGACTTCTGCGGAGTAGTCGCACGTTGCCGCCACTTTGGATTTTGGCGCACCTTTTGGCATCACCACTTTACCGTCGATACCCAGCAt                                   <  2:3332570/93‑1 (MQ=255)

                                                       aGAACGACTTCTGCGGAGTAGTCGCACGTTGCCGCCACTTTGGATTTTGGCGCACCTTTTGGCATCACCACTTTACCGTCGATACCCAGCATc                                  >  2:241618/1‑93 (MQ=255)

                                                       aGAACGACTTCTGCGGAGTAGTCGCACGTTGCCGCCACTTTGGATTTTGGCGCACCTTTTGGCATCACCACTTTACCGTCGATACCCAGCATc                                  <  2:3896624/93‑1 (MQ=255)

                                                       aGAACGACTTCTGCGGAGTAGTCGCACGTTGCCGCCACTTTGGATTTTGGCGCACCTTTTGGCATCACCACTTTACCGTCGATACCCAGCATc                                  >  1:2648190/1‑93 (MQ=255)

                                                       aGAACGACTTCTGCGGAGTAGTCGCACGTTGCCGCCACTTTGGATTTTGGCGCACCTTTTGGCATCACCACTTTACCGTCGATACCCAGCATc                                  <  2:3612748/93‑1 (MQ=255)

                                                        gAACGACTTCTGCGGAGTAGTCGCACGTTGCCGCCACTTTGGATTTTGGCGCACCTTTTGGCATCACCACTTTACCGTCGATACCCAGCATcg                                 >  1:1362830/1‑93 (MQ=255)

                                                          aCGACTTCTGCGGAGTAGTCGCACGTTGCCGCCACTTTGGATTTTGGCGCACCTTTTGGCATCACCACTTTACCGTCGATACCCAGCATcgcg                               >  2:4236270/1‑93 (MQ=255)

                                                           cGACTTCTGCGGAGTAGTCGCACGTTGCCGCCACTTTGGATTTTGGCGCACCTTTTGGCATCACCACTTTACCGTCGATACCCAGCATCgcgc                              <  2:4126447/93‑1 (MQ=255)

                                                            gACTTCGGCGGAGTAGTCGCACGTTGCCGCCACTTTTGATTTTGGCGCACCTTTTGGCATCACCACTTTACCGTCGATACCCAGCATCGCGCa                             <  1:3099723/93‑1 (MQ=255)

                                                             aCTTCTGCGGAGTAGTCGCACGTTGCCGCCACTTTGGATTTTGGCGCACCTTTTGGCATCACCACTTTACCGTCGATACCCAGCATCGCGCAg                            <  2:2021215/93‑1 (MQ=255)

                                                             aCTTCTGCGGAGTAGTCGCACGTTGCCGCCACTTTGGATTTTGGCGCACCTTTTGGCATCACCACTTTACCGTCGATACCCAGCATCGCGCAg                            >  2:561326/1‑93 (MQ=255)

                                                              cTTCTGCGGAGTAGTCGCACGTTGCCGCCACTTTGGATTTTGGCGCACCTTTTGGCATCACCACTTTACCGTCGATACCCAGCATCGCGCAgg                           <  1:934666/93‑1 (MQ=255)

                                                              cTTCTGCGGAGTAGTCGCACGTTGCCGCCACTTTGGATTTTGGCGCACCTTTTGGCATCACCACTTTACCGTCGATACCCAGCATCGCGCAgg                           >  1:4048922/1‑93 (MQ=255)

                                                                  tGCGGAGTAGTCGCACGTTGCCGCCACTTTGGATTTTGGCGCACCTTTTGGCATCACCACTTTACCGTCGATACCCAGCATCGCGCAGGagag                       >  1:2801972/1‑93 (MQ=255)

                                                                  tGCGGAGTAGTCGCACGTTGCCGCCACTTTGGATTTTGGCGCACCTTTTGGCATCACCACTTTACCGTCGATACCCAGCATCGCGCAGGagag                       <  2:2289397/93‑1 (MQ=255)

                                                                  tGCGGAGTAGTCGCACGTTGCCGCCACTTTGGATTTTGGCGCACCTTTTGGCATCACCACTTTACCGTCGATACCCAGCATCGCGCAGGagag                       <  2:4050074/93‑1 (MQ=255)

                                                                    gggAGTAGTCGCACGTTGCCGCCACTTTGGATTTTGGCGCACCTTTTGGCATCACCACTTTACCGTCGATACCCAGCATCGCGCAGGAGAggg                     <  1:75158/92‑1 (MQ=255)

                                                                       ggtagtCGCCGGTTGCCGCCACTTTGGATTTTGGCGCACCTTTTGGCATCACCACTTTACCGTCGATACCCAGCATCGCGCAGGAGAGGGaaa                  <  1:1081170/92‑1 (MQ=255)

                                                                        gtagtCGCACGTTGCCGCCACTTTGGATTTTGGCGCACCTTTTGGCATCACCACTTTACCGTCGATACCCAGCATCGCGCAGGAGAGGGAAAc                 >  2:3778796/1‑93 (MQ=255)

                                                                           gtCGCACGTTGCCGCCACTTTGGATTTTGGCGCACCTTTTGGCATCACCACTTTACCGTCGATACCCAGCATCGCGCAGGAGAGGGAAAcccc              >  1:2925835/1‑93 (MQ=255)

                                                                           gtCGCACGTTGCCGCCACTTTGGATTTTGGCGCACCTTTTGGCATCACCACTTTACCGTCGATACCCAGCATCGCGCAGGAGAGGGAAAcccc              <  1:2707265/93‑1 (MQ=255)

                                                                           gtCGCACGTTGCCGCCACTTTGGATTTTGGCGCACCTTTTGGCATCACCACTTTACCGCCGATACCCAGCATAGCGCAGGAGTGGGAAAcccc              >  2:1165400/1‑93 (MQ=255)

                                                                            tCGCACGTTGCCGCCACTTTGGATTTTGGCGCACCTTTTGGCATCACCACTTTACCGTCGATACCCAGCATCGCGCAGGAGAGGGAAACCCCt             <  1:1249533/93‑1 (MQ=255)

                                                                            tCGCACGTTGCCGCCACTTTGGATTTTGGCGCACCTTTTGGCATCACCACTTTACCGTCGATACCCAGCATCGCGCAGGAGAGGGAAACCCCt             <  2:1633393/93‑1 (MQ=255)

                                                                               cACGTTGCCGCCACTTTGGATTTTGGCGCACCTTTTGGCATCACCACTTTACCGTCGATACCCAGCATCGCGCAGGAGAGGGAAACCCCTTgc          >  2:3164515/1‑93 (MQ=255)

                                                                               cACGTTGCCGCCACTTTGGATTTTGGCGCACCTTTTGGCATCACCACTTTACCGTCGATACCCAGCATCGCGCAGGAGAGGGAAACCCCTTgc          >  2:1892419/1‑93 (MQ=255)

                                                                               cACGTGTCCGCCACTGTGGATTTTGGCGCACCTTTTGGCATCACCACTTTACCGTCGATACCCAGCATCGCGCAGGAGAGGGAAACCCCTTgc          <  1:2320136/93‑1 (MQ=255)

                                                                                atgTTGCCGCCACTTTGGATTTTGGCGCACCTTTTGGCATCACCACTTTACCGTCGATACCCAGCATCGCGCAGGAGAGGGAAACCCCTTgcg         >  2:4667445/3‑93 (MQ=255)

                                                                                aCGTTGCCGCCACTTTGGATTTTGGCGCACCTTTTGGCATCACCACTTTACCGTCGATACCCAGCATCGCGCAGGAGAGGGAAACCCCTTgcg         >  2:1542330/1‑93 (MQ=255)

                                                                                aCGTTGCCGCCACTTTGGATTTTGGCGCACCTTTTGGCATCACCACTTTACCGTCGATACCCAGCATCGCGCAGGAGAGGGAAACCCCTTgcg         >  1:1129677/1‑93 (MQ=255)

                                                                                  gTTGCCGCCACTTTGGATTTTGGCGCACCTTTTGGCATCACCACTTTACCGTCGATACCCAGCATCGCGCAGGAGAGGGAAACCCCTTGCGCa       >  1:1763862/1‑93 (MQ=255)

                                                                                    tGCCGCCACTTTGGATTTTGGCGCACCTTTTGGCATCACCACTTTACCGTCGATACCCAGCATCGCGCAGGAGAGGGAAACCCCTTGCGCATg     <  1:2152875/93‑1 (MQ=255)

                                                                                     gccgccACTTTGGATTTTGGCGCACCTTTTGGCATCACCACTTTACCGTCGATACCCAGCATCGCGCAGGAGAGGGAAACCCCTTGCGCATgg    <  1:2047233/93‑1 (MQ=255)

                                                                                      ccGCCACTTTGGATTTTGGCGCACCTTTTGGCATCACCACTTTACCGTCGATACCCAGCATCGCGCAGGAGAGGGAAACCCCTTGCGCATGGt   <  1:4565319/93‑1 (MQ=255)

CATTTCGACAATTTCGCTCACTTTAGCGATAGTGTCGTTGAAGTTATCACCATGCAGAACGACTTCTGCGGAGTAGTCGCACGTTGCCGCTACTTTGGATTTTGGCGCACCTTTTGGCATCACCACTTTACCGTCGATACCCAGCATCGCGCAGGAGAGGGAAACCCCTTGCGCATGGTT  >  NC_000913/3263238‑3263417

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 19 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`