BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,273,491	T→C	V190A (GTA→GCA)	yhaG →	putative hydrolase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,273,491	0	T	C	95.5%	517.6 / 7.7	178	V190A (GTA→GCA)	yhaG	putative hydrolase
Reads supporting (aligned to +/- strand): ref base T (4/4); new base C (65/105); total (69/109)
Fisher's exact test for biased strand distribution p-value = 7.13e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.99e-01

Only 100 of 188 total aligned reads displayed.

TGCTACCGAAATACCCGAACGTCGATGGCGTGGTGGGGCTGAATCATTTGTACGGTTGTGGCGTGGCGATTAACGCACCGGCGGCAGTTGTACCTATCCGTACCATTCACAATATTTCGCTGAATCCTAACTTTGGCGGCGAAGTAATGGTGATTGGCCTGGGTTGTGAAAAGTTGCAGCCT  >  NC_000913/3273401‑3273582

tGCTACCGAAATACCCGAACGTCGATGGCGTGGTGGGGCTGAATCATTTGTACGGTTGTGGCGTGGCGATTAACGCACCGGCGGCAGTTGCAc                                                                                           >  1:4175688/1‑93 (MQ=255)

tGCTACCGAAATACCCGAACGTCGATGGCGTGGTGGGGCTGAATCATTTGTACGGTTGTGGCGTGGCGATTAACGCACCGGCGGCAGTTGCAc                                                                                           >  2:4162588/1‑93 (MQ=255)

tGCTACCGAAATACCCGAACGTCGATGGCGTGGTGGGGCTGAATCATTTGTACGGTTGTGGCGTGGCGATTAACGCACCGGCGGCAGTTGCAc                                                                                           >  1:779922/1‑93 (MQ=255)

 gCTACCGAAATACCCGAACGTCGATGGCGTGGTGGGGCTGAATCATTTGTACGGTTGTGGCGTGGCGATTAACGCACCGGCGGCAGTTGCAcc                                                                                          <  2:2097351/93‑1 (MQ=255)

  cTACCGAAATACCCGAACGTCGATGGCGTGGTGGGGCTGAATCATTTGTACGGTTGTGGCGTGGCGATTAACGCACCGGCGGCAGTTGCACCt                                                                                         <  2:1883634/93‑1 (MQ=255)

     ccGAAATACCCGAACGTCGATGGCGTGGTGGGGCTGAATCATTTGTACGGTTGTGGCGTGGCGATTAACGCACCGGCGGCAGTTGCACCTATc                                                                                      <  1:204203/93‑1 (MQ=255)

     ccGAAATACCCGAACGTCGATGGCGTGGTGGGGCTGAATCATTTGTACGGTTGTGGCGTGGCGATTAACGCACCGGCGGCAGTTGCACCTATc                                                                                      >  1:1653739/1‑93 (MQ=255)

      cGAAATACCCGAACGTCGATGGCGTGGTGGGGCTGAATCATTTGTACGGTTGTGGCGTGGCGATTAACGCACCGGCGGCAGTTGCACCTATcc                                                                                     <  1:896255/93‑1 (MQ=255)

          aTACCCGAACGTCGATGGCGTGGTGGGTCTGAATCATTTGTACGGTTGTGGCGTGGCTATTAACGCACCGGCGGCAGTTGCACCTATCCGTAc                                                                                 <  1:3273006/93‑1 (MQ=255)

             cccGAACGTCGATGGCGTGGTGGGGCTGAATCATTTGTACGGTTGTGGCGTGGCGATTAACGCACCGGCGGCCGTTGCACCTATCCGTCCCAt                                                                              >  1:1308145/1‑93 (MQ=255)

              ccGAACGTCGATGGCGTGGTGGGGCTGAATCATTTGTACGGTTGTGGCGTGGCGATTAACGCACCGGCGGGAGTTGCACCTATCCGTACCCtt                                                                             >  2:897598/1‑93 (MQ=255)

              ccGAACGTCGATGGCGTGGTGGGGCTGAATCATTTGTACGGTTGTGGCGTGGCGATTAACGCACCGGCGGCAGTTGCACCTATCCGTACCAtt                                                                             >  1:1926231/1‑93 (MQ=255)

              ccGAACGTCGATGGCGTGGTGGGGCTGAATCATTTGTACGGTTGTGGCGTGGCGATTAACGCACCGGCGGCAGTTGCACCTATCCGTACCAtt                                                                             >  2:3699701/1‑93 (MQ=255)

              ccGAACGTCGATGGCGTGGTGGGGCTGAATCATTTGTACGGTTGTGGCGTGGCGATTAACGCACCGGCGGCAGTTGCACCTATCCGTACCAtt                                                                             <  1:644900/93‑1 (MQ=255)

               cGAACGTCGATGGCGTGGTGGGGCTGAATCATTTGTACGGTTGTGGCGTGGCGATTAACGCACCGGCGGCAGTTGCACCTATCCGTACCATTc                                                                            <  2:117383/93‑1 (MQ=255)

               cGAACGTCGATGGCGTGGTGGGGCTGAATCATTTGTACGGTTGTGGCGTGGCGATTAACGCACCGGCGGCAGTTGCACCTATCCGTACCATTc                                                                            >  2:2332142/1‑93 (MQ=255)

               cGAACGTCGATGGCGTGGTGGGGCTGAATCATTTGTACGGTTGTGGCGTGGCGATTAACGCACCGGCGGCAGTTGCACCTATCCGTACCATTc                                                                            >  2:1450780/1‑93 (MQ=255)

                gAACGTCGATGGCGTGGTGGGGCTGAATCATTTGTACGGTTGTGGCGTGGCGATTAACGCACCGGCGGCAGTTGCACCTATCCGTACCATTca                                                                           <  2:2022057/93‑1 (MQ=255)

                 aaCGTCGATGGCGTGGTGGGGCTGAATCATTTGTACGGTTGTGGCGTGGCGATTAACGCACCGGCGGCAGTTGCACCTATCCGTACCATTcac                                                                          <  1:112137/93‑1 (MQ=255)

                  aCGTCGATGGCGTGGTGGGGCTGAATCATTTGTACGGTTGTGGCGTGGCGATTAACGCACCGGCGGCAGTTGCACCTATCCGTACCATTcaca                                                                         <  1:211712/93‑1 (MQ=255)

                  aCGTCGATGGCGTGGTGGGGCTGAATCATTTGTACGGTTGTGGCGTGGCGATTAACGCACCGGCGGCAGTTGCACCTATCCGTACCATTcaca                                                                         <  2:1759645/93‑1 (MQ=255)

                   cGTCGATGGCGTGGTGGGGCTGAATCATTTGTACGGTTGTGGCGTGGCGATTAACGCACCGGCGGCAGTTGCACCTATCCGTACCATTCACaa                                                                        <  1:4638708/93‑1 (MQ=255)

                    gTCGATGGCGTGGTGGGGCTGAATCATTTGTACGGTTGTGGCGTGGCTATTAACGCACCGGCGGCAGTTGTCCCTATCCGTACCATTCACAat                                                                       <  2:2752570/93‑1 (MQ=255)

                      cGATGGCGTGGTGGGGCTGAATCATTTGTACGGTTGTGGCGTGGCGATTAACGCACCGGCGGCAGTTGCACCTATCCGTACCATTCACAatat                                                                     <  2:4481158/93‑1 (MQ=255)

                       gATGGCGTGGTGGGGCTGAATCATTTGTACGGTTGTGGCGTGGCGATTAACGCACCGGCGGCAGTTGCACCTATCCGTACCATTCACAATAtt                                                                    >  2:2800266/1‑93 (MQ=255)

                       gATGGCGTGGTGGGGCTGAATCATTTGTACGGTTGTGGCGTGGCGATTAACGCACCGGCGGCAGTTGCACCTATCCGTACCATTCACAATAtt                                                                    <  1:933886/93‑1 (MQ=255)

                        aTGGCGTGGTGGGGCTGAATCATTTGTACGGTTGTGGCGTGGCGATTAACGCACCGGCGGCAGTTGCACCTATCCGTACCATTCACAATAttt                                                                   >  1:1732863/1‑93 (MQ=255)

                        aTGGCGTGGTGGGGCTGAATCATTTGTACGGTTGTGGCGGGGCGATTAACGCACCGGCGGCAGTTGCACCTATCCGTACCATTCACAATAttt                                                                   >  2:1865042/1‑93 (MQ=255)

                         tGGCGTGGTGGGGCTGAATCATTTGTACGGTTGTGGCGTGGCGATTAACGCACCGGCGGCAGTTGCACCTATCCGTACCATTCACAATATTTc                                                                  <  1:896997/93‑1 (MQ=255)

                         tGGCGTGGGGGGGCTGAATCATTTGTACGGTTGTGGCGTGGCGATTAAAGCACCGGCGGCAGTTGCACCTATCCGGACCATTCACAATATTTc                                                                  >  2:2494633/1‑93 (MQ=255)

                          ggCGTGGTGGGGCTGAATCATTTGTACGGTTGTGGCGTGGCGATTAACGCACCGGCGGCAGTTGCACCTATCCGTACCATTCACAATATTTCg                                                                 >  1:1636021/1‑93 (MQ=255)

                           gCGTGGTGGGGCTGAATCATTTGTACGGTTGTGGCGTGGCGATTAACGCACCGGCGGCAGTTGCACCTATCCGTACCATTCACAATATTTCGc                                                                <  1:4246421/93‑1 (MQ=255)

                            cGTGGTGGGGCTGAATCATTTGTACGGTTGTGGCGTGGCGATTAACGCACCGGCGGCAGTTGCACCTATCCGTACCATTCACAATATTTCGCt                                                               >  1:983358/1‑93 (MQ=255)

                               gctggGGCTGAATCATTTGTACGGTTGTGGCGTGGCAATTAACGCACCGGCGGCAGTTGCACCTATCCGTACCATTCACAATATTTCGCTGaa                                                            <  1:2478742/91‑1 (MQ=255)

                                gtggGGCTGAATCATTTGTACGGTTGTGGCGTGGCGATTAACGCACCGGCGGCAGTTGCACCTATCCGTACCATTCACAATATTTCGCTGAAt                                                           <  2:4388565/93‑1 (MQ=255)

                                 tggGGCTGAATCATTTGTACGGTTGTGGCGTGGCGATTAACGCACCGGCGGCAGTTGCACCTATCCGTACCATTCACAATATTTCGCTGAATc                                                          >  2:2393278/1‑93 (MQ=255)

                                 tggGGCTGAATCATTTGTACGGTTGTGGCGTGGCGATTAACGCACCGGCGGCAGTTGCACCTATCCGTACCATTCACAATATTTCGCTGAATc                                                          <  2:1431219/93‑1 (MQ=255)

                                  ggggCTGAATCATTTGTACGGTTGTGGCGTGGCGATTAACGCACCGGCGGCAGTTGCACCTATCCGTACCATTCACAATATTTCGCTGAATcc                                                         >  1:2800792/1‑93 (MQ=255)

                                  ggggCTGAATCATTTGTACGGTTGTGGCGTGGCGATTAACGCACCGGCGGCAGTTGCACCTATCCGTACCATTCACAATATTTCGCTGAATcc                                                         <  1:151888/93‑1 (MQ=255)

                                  ggggCTGAATCATTTGTACGGTTGTGGCGTGGCGATTAACGCACCGGCGGCAGTTGCACCTATCCGTACCATTCACAATATTTCGCTGAATcc                                                         <  1:2921579/93‑1 (MQ=255)

                                  ggggCTGAATCACTTGTACGGTTGTGGCGTGGCGATTAACGCACCGGCGGCAGTTGCACCTATCCGTACCATTCACAATATTTCGCTGAATcc                                                         <  1:1983922/93‑1 (MQ=255)

                                       tGAATCATTTGTACGGTTGTGGCGTGGCGATTAACGCACCGGCGGCAGTTGCACCTATCCGTACCATTCACAATATTTCGCTGCATCCTAACt                                                    >  2:4344410/1‑93 (MQ=255)

                                        taaTCGTTTGTACGGTTGTGGCGTGGCGATTAACGGTCCGGCGGCAGTTGCACCTATCCGTACCATTCACAATATTTCGCTGAATCCTAACtt                                                   <  1:1154768/92‑1 (MQ=255)

                                        gAATCATTTGTACGGTTGTGGCGTGGCGATTAACGCACCGGCGGCAGTTGCACCTATCCGTACCATTCACAATATTTCGCTGAATCCTAACtt                                                   >  2:1709139/1‑93 (MQ=255)

                                        gAATCATTTGTACGGTTGTGGCGTGGCGATTAACGCACCGGCGGCAGTTGCACCTATCCGTACCATTCACAATATTTCGCTGAATCCTAACtt                                                   <  2:3344855/93‑1 (MQ=255)

                                        gAATCATTTGTACGGTTGTGGCGTGGCGATTAACGCACCGGCGGCAGTTGCACCTATCCGTACCATTCACAATATTTCGCTGAATCCTAACtt                                                   <  1:2208216/93‑1 (MQ=255)

                                         aaTCATTTGTACGGTTGTGGCGTGGCGATTAACGCACCGGCGGCAGTTGCACCTATCCGTACCATTCACAATATTTCGCTGAATCCTAACttt                                                  <  1:3360911/93‑1 (MQ=255)

                                          aTCATTTGTACGGTTGTGGCGTGGCGATTAACGCACCGGCGGCAGTTGCACCTATCCGTACCATTCACAATATTTCGCTGAATCCTAACTTTg                                                 <  2:3467599/93‑1 (MQ=255)

                                            cATTTGTACGGTTGTGGCGTGGCGATTAACGCACCGGCGGCAGTTGCACCTATCCGTACCATTCACAATATTTCGCTGAATCCTAACTTTggc                                               <  2:4128106/93‑1 (MQ=255)

                                            cATTTGTACGGTTGTGGCGTGGCGATTAACGCACCGGCGGCAGTTGCACCTATCCGTACCATTCACAATATTTCGCTGAATCCTAACTTTggc                                               <  2:3831152/93‑1 (MQ=255)

                                             aTTTGTACGGTTGTGGCGTGGCGATTAACGCACCGGCGGCAGTTGCACCTATCCGTACCATTCACAATATTTCGCTGAATCCTAACTTTggcg                                              >  2:2120561/1‑93 (MQ=255)

                                             aTTTGTACGGTTGTGGCGTGGCGATTAACGCACCGGCGGCAGTTGCACCTATCCGTACCATTCACAATATTTCGCTGAATCCTAACTTTggcg                                              >  1:3503658/1‑93 (MQ=255)

                                             aTTTGTACGGTTGTGGCGTGGCGATTAACGCACCGGCGGCAGTTGCACCTATCCGTACCATTCACAATATTTCGCTGAATCCTAACTTTggcg                                              >  2:1072230/1‑93 (MQ=255)

                                              tttGTACGGTTGTGGCGTGGCGATTAACGCACCGGCGGCAGTTGCACCTATCCGTACCATTCACAATATTTCGCTGAATCCTAACTTTggcgg                                             >  2:3126759/1‑93 (MQ=255)

                                              tttGTACGGTTGTGGCGTGGCGATTAACGCACCGGCGGCAGTTGCACCTATCCGTACCATTCACAATATTTCGCTGAATCCTAACTTTggcgg                                             >  1:3569396/1‑93 (MQ=255)

                                               ttGTACGGTTGTGGCGTGGCGATTAACGCACCGGCGGCAGTTGCACCTATCCGTACCATTCACAATATTTCGCTGAATCCTAACTTTggcggc                                            <  2:3390346/93‑1 (MQ=255)

                                                  tACGGTTGTGGCGTGGCGATTAACGCACCGGCGGCAGTTGCACCTATCCGTACCATTCACAATATTTCGCTGAATCCTAACTTTGGCGGCGaa                                         <  2:70464/93‑1 (MQ=255)

                                                   aCGGTTGTGGCGTGTCGATTAACGCACCGGCGGCAGTTGCACCTATCCGTACCATTCACAATATTTCGCTGAATCCTAACTTTGGCGGCGAAg                                        <  2:2696873/93‑1 (MQ=255)

                                                   aCGGTTGTGGCGTGGCGATTAACGCACCGGCGGCAGTTGCACCTATCCGTACCATTCACAATATTTCGCTGAATCCTAACTTTGGCGGCGAAg                                        <  2:1161970/93‑1 (MQ=255)

                                                    cGGTTGTGGCGTGGCGATTAACGCACCGGCGGCAGTTGCACCTATCCGTACCATTCACAATATTTCGCTGAATCCTAACTTTGGCGGCGAAGt                                       <  1:2335245/93‑1 (MQ=255)

                                                    cGGTTGTGGCGTGGCGATTAACGCACCGGCGGCAGTTGCACCTATCCGTACCATTCACAATATTTCGCTGAATCCTAACTTTGGCGGCGAAGt                                       <  1:3466183/93‑1 (MQ=255)

                                                      gTTGTGGCGTGGCGATTAACGCACCGGCGGCAGTTGCACCTATCCGTACCATTCACAATATTTCGCTGAATCCTAACTTTGGCGGCGAAGTaa                                     <  2:3147111/93‑1 (MQ=255)

                                                       ttGTGGCGTGGCGATTAACGCACCGGCGGCAGTTGCACCTATCCGTACCATTCACAATATTTCGCTGAATCCTAACTTTGGCGGCGAAGTAAt                                    <  1:49005/93‑1 (MQ=255)

                                                       ttGTGGCGTGGCGATTAACGCACCGGCGGCAGTTGCACCTATCCGTACCATTCACAATATTTCGCTGAATCCTAACTTTGGCGGCGAAGTAAt                                    <  1:578823/93‑1 (MQ=255)

                                                       ttGTGGCGTGGCGATTAACGCACCGGCGGCAGTTGCACCTATCCGTACCATTCACAATATTTCGCTGAATCCTAACTTTGGCGGCGAAGTAAt                                    <  1:2394382/93‑1 (MQ=255)

                                                       ttGTGGCGTGGCGATTAACGCACCGGCGGCAGTTGCACCTATCCGTACCATTCACAATATTTCGCTGAATCCTAACTTTGGCGGCGAAGTAAt                                    <  1:3411491/93‑1 (MQ=255)

                                                          tggcgtggcgATTAACGCACCGGCGGCAGTTGCACCTATCCGTACCATTCACAATATTTCGCTGAATCCTAACTTTGGCGGCGAAGTAATGGt                                 >  1:3822403/1‑93 (MQ=255)

                                                          tggcgtggcgATTAACGCACCGGCGGCAGTTGCACCTATCCGTACCATTCACAATATTTCGCTGAATCCTAACTTTGGCGGCGAAGTAATGGt                                 <  1:1105986/93‑1 (MQ=255)

                                                           ggcgtggcgATTAACGCACCGGCGGCAGTTGCACCTATCCGTACCATTCACAATATTTCGCTGAATCCTAACTTTGGCGGCGAAGTAATGGTg                                <  2:2205398/93‑1 (MQ=255)

                                                            gcgtggcgATTAACGCACCGGCGGCAGTTGCACCTATCCGTACCATTCACAATATTTCGCTGAATCCTAACTTTGGCGGCTAAGTAATGGTGa                               <  2:1557906/93‑1 (MQ=255)

                                                            gcgtggcgATTAACGCACCGGCGGCAGTTGCACCTATCCGTACCATTCACAATATTTCGCTGAATCCTAACTTTGGCGGCGAAGTAATGGTGa                               >  1:2993261/1‑93 (MQ=255)

                                                             cgtggcgATTAACGCACCGGCGGCAGTTGCACCTATCCGTACCATTCACAATATTTCGCTGAATCCTAACTTTGGCGGCGAAGTAATGGTGAt                              <  1:13975/93‑1 (MQ=255)

                                                             cgtggcgATTAACGCACCGGCGGCAGTTGCACCTATCCGTACCATTCACAATATTTCGCTGAATCCTAACTTTGGCGGCGAAGTAATGGTGAt                              <  2:3485517/93‑1 (MQ=255)

                                                              gtggcgATTAACGCACCGGCGGCAGTTGCACCTATCCGTACCATTCACAATATTTCGCTGAATCCTAACTTTGGCGGCGAAGTAATGGTGAtt                             <  1:1765974/93‑1 (MQ=255)

                                                               tggcgATTAACGCACCGGCGGCAGTTGCACCTATCCGTACCATTCACAATATTTCGCTGAATCCTAACTTTGGCGGCGAAGTAATGGTGATTg                            >  2:4374402/1‑93 (MQ=255)

                                                               tggcgATTAACGCACCGGCGGCAGTTGCACCTATCCGTACCATTCACAATATTTCGCTGAATCCTAACTTTGGCGGCGAAGTAATGGTGATTg                            <  1:3744936/93‑1 (MQ=255)

                                                                 gcgATTAACGCACCGGCGGCAGTTGCACCTATCCGTACCATTCACAATATTTCGCTGAATCCTAACTTTGGCGGCGAAGTAATGGTGATTGGc                          <  2:2321559/93‑1 (MQ=255)

                                                                  cgATTAACGCACCGGCGGCAGTTGCACCTATCCGTACCATTCACAATATTTCGCTGAATCCTAACTTTGGCGGCGAAGTAATGGTGATTGGcc                         <  2:1901467/93‑1 (MQ=255)

                                                                  cgATTAACGCACCGGCGGCAGTTGCACCTATCCGTACCATTCACAATATTTCGCTGAATCCTAACTTTGGCGGCGAAGTAATGGTGATTGGcc                         <  2:3600401/93‑1 (MQ=255)

                                                                   gATTAACGCACCGGCGGCAGTTGCACCTATCCGTACCATTCACAATATTTCGCTGAATCCTAACTTTGGCGGCGAAGTAATGGTGATTGGCCt                        <  2:3247893/93‑1 (MQ=255)

                                                                   gATTAACGCACCGGCGGCAGTTGCACCTATCCGTACCATTCACAATATTTCGCTGAATCCTAACTTTGGCGGCGAAGTAATGGTGATTGGCCt                        >  1:3601364/1‑93 (MQ=255)

                                                                     ttAACGCACCGGCGGCAGTTGCACCTATCCGTACCATTCACAATATTTCGCTGAATCCTAACTTTGGCGGCGAAGTAATGGTGATTGGCCTgg                      >  2:2027048/1‑93 (MQ=255)

                                                                     ttAACGCACCGGCGGCAGTTGCACCTATCCGTACCATTCACAATATTTCGCTGAATCCTAACTTTGGCGGCGAAGTAATGGTGATTGGCCTgg                      <  2:1212914/93‑1 (MQ=255)

                                                                     ttAACGCACCGGCGGCAGTTGCACCTATCCGTACCATTCACAATATTTCGCTGAATCCTAACTTTGGCGGCGAAGTAATGGTGATTGGCCTgg                      <  1:3134475/93‑1 (MQ=255)

                                                                       aaCGCACCGGCGGCAGTTGCACCTATCCGTACCATTCACAATATTTCGCTGAATCCTAACTTTGGCGGCGAAGTAATGGTGATTGGCCTGGGt                    <  2:4016740/93‑1 (MQ=255)

                                                                        aCGCACCGGCGGCAGTTGCACCTATCCGTACCATTCACAATATTTCGCTGAATCCTAACTTTGGCGGCGAAGTAATGGTGATTGGCCTGGGtt                   <  2:612165/93‑1 (MQ=255)

                                                                          gCACCGGCGGCAGTTGCACCTATCCGTACCATTCACAATATTTCGCTGAATCCTAACTTTGGCGGCGAAGTAATGGTGATTGGCCTGGGTtgt                 <  2:3792658/93‑1 (MQ=255)

                                                                          gCACCGGCGGCAGTTGCACCTATCCGTACCATTCACAATATTTCGCTGAATCCTAACTTTGGCGGCGAAGTAATGGTGATTGGCCTGGGTtgt                 <  1:3984665/93‑1 (MQ=255)

                                                                          gCACCGGCGGCAGTTGCACCTATCCGTACCATTCACAATATTTCGCTGAATCCTAACTTTGGCGGCGAAGTAATGGTGATTGGCCTGGGTtgt                 >  1:4277602/1‑93 (MQ=255)

                                                                            aCCGGCGGCAGTTGCACCTATCCGTACCATTCACAATATTTCGCTGAATCCTAACTTTGGCGGCGAAGTAATGGTGATTGGCCTGGGTTGTGa               >  2:523918/1‑93 (MQ=255)

                                                                             ccGGCGGCAGTTGCACCTATCCGTACCATTCACAATATTTCGCTGAATCCTAACTTTGGCGGCGAAGTAATGGTGATTGGCCTGGGTTGTGaa              >  1:3923234/1‑93 (MQ=255)

                                                                               ggcggcAGTTGCACCTATCCGTACCATTCACAATATTTCGCTGAATCCTAACTTTGGCGGCGAAGTAATGGTGATTGGCCTGGGTTGTGaaaa            >  2:2172413/1‑93 (MQ=255)

                                                                                 cggcAGTTGCACCTATCCGTACCATTCACAATATTTCGCTGAATCCTAACTTTGGCGGCGAAGTAATGGTGATTGGCCTGGGTTGTGAAAAGt          <  2:2845107/93‑1 (MQ=255)

                                                                                  ggcAGTTGCACCTATCCGTACCATTCACAATATTTCGCTGAATCCTAACTTTGGCGGCGAAGTAATGGTGATTGGCCTGGGTTGTGAAAAGtt         <  2:3642005/93‑1 (MQ=255)

                                                                                   gcAGTTGCACCTATCCGTACCATTCACAATATTTCGCTCAATCCTAACTTTGGCGGCGAAGTAATGGTGATTGGCCTGGGTTGTGAAAAGGTg        >  2:1524422/1‑93 (MQ=255)

                                                                                      gTTGCACCTATCCGTACCATTCACAATATTTCGCTGAATCCTAACTTTGGCGGCGAAGTAATGGTGATTGGCCTGGGTTGTGAAAAGTTGCAg     >  2:2443018/1‑93 (MQ=255)

                                                                                      gTTGCACCTATCCGTACCATTCACAATATTTCGCTGAATCCTAACTTTGGCGGCGAAGTAATGGTGATTGGCCTGGGTTGTGAAAAGTTGCAg     >  2:815336/1‑93 (MQ=255)

                                                                                      gTTGCACCTATCCGTACCATTCACAATATTTCGCTGAATCCTAACTTTGGCGGCGAAGTAATGGTGATTGGCCTGGGTTGTGAAAAGATGCAg     >  2:3319516/1‑93 (MQ=255)

                                                                                       ttGCACCTATCCGTACCATTCACAATATTTCGCTGAATCCTAACTTTGGCGGCGAAGTAATGGTGATTGGCCTGGGTTGTGAAAAGTTGCAGc    >  2:3250303/1‑93 (MQ=255)

                                                                                         gTACCTATCCGTACCATTCACAATATTTCGCTGAATCCTAACTTTGGCGGCGAAGTAATGGTGATTGGCCTGGGTTGTGAAAAGTTGCAGCCt  >  2:4242384/1‑93 (MQ=255)

TGCTACCGAAATACCCGAACGTCGATGGCGTGGTGGGGCTGAATCATTTGTACGGTTGTGGCGTGGCGATTAACGCACCGGCGGCAGTTGTACCTATCCGTACCATTCACAATATTTCGCTGAATCCTAACTTTGGCGGCGAAGTAATGGTGATTGGCCTGGGTTGTGAAAAGTTGCAGCCT  >  NC_000913/3273401‑3273582

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 19 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`