BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	32,257	A→G	I481V (ATC→GTC)	carB →	carbamoyl‑phosphate synthase large subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	32,257	0	A	G	100.0%	501.3 / NA	158	I481V (ATC→GTC)	carB	carbamoyl‑phosphate synthase large subunit
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (78/80); total (78/80)

Only 100 of 167 total aligned reads displayed.

GCGTCTTCAACCTGACCAACATTGACCGCTGGTTCCTGGTACAGATTGAAGAGCTGGTGCGTCTGGAAGAGAAAGTGGCGGAAGTGGGCATCACTGGCCTGAACGCTGACTTCCTGCGCCAGCTGAAACGCAAAGGCTTTGCCGATGCGCGCTTGGCAAAACTGGCGGGCGTACGCGAA  >  NC_000913/32168‑32346

gCGTCTTCAACCTGACCAACATTGACCGCTGGTTCCTGGTACAGATTGAAGAGCTGGTGCGTCTGGAAGAGAAAGTGGCGGAAGTGGGCGTCa                                                                                        >  1:3810400/1‑93 (MQ=255)

gCGTCTTCAACCTGACCAACATTGACCGCTGGTTCCTGGTACAGATTGAAGAGCTGGTGCGTCTGGAAGAGAAAGTGGCGGAAGTGGGCGTCa                                                                                        <  2:2162381/93‑1 (MQ=255)

gCGTCTTCAACCTGACCAACATTGACCGCTGGTTCCTGGTACAGATTGAAGAGCTGGGGCGTCTGGAAGAGAAAGTGGCGGAAGGGGGCGTCa                                                                                        >  1:918350/1‑93 (MQ=255)

 cGTCTTCAACCTGACCAACATTGACCGCTGGTTCCTGGTACAGATTGAAGAGCTGGTGCGTCTGGAAGAGAAAGTGGCGGAAGTGGGCGTCAc                                                                                       <  2:248364/93‑1 (MQ=255)

  gTCTTCAACCTGACCAACATTGACCGCTGGTTCCTGGTACAGATTGAAGAGCTGGTGCGTCTGGAAGAGAAAGTGGCGGAAGTGGGCGTCACt                                                                                      >  1:3344424/1‑93 (MQ=255)

  gTCTTCAACCTGACCAACATTGACCGCTGGTTCCTGGTACAGATTGAAGAGCTGGTGCGTCTGGAAGAGAAAGTGGCGGAAGTGGGCGTCACt                                                                                      <  1:863067/93‑1 (MQ=255)

  gTCTTCAACCTGACCAACATTGACCGCTGGTTCCTGGTACAGATTGAAGAGCTGGTGCGTCTGGAAGAGAAAGTGGCGGAAGTGGGCGTCACt                                                                                      <  1:1301507/93‑1 (MQ=255)

   tCTTCAACCTGACCAACATTGACCGCTGGTTCCTGGTACAGATTGAAGAGCTGGTGCGTCTGGACGAGCAAGTGGCGGAAGTGGGCGTCATTg                                                                                     >  1:689132/1‑93 (MQ=255)

     ttCAACCTGACCAACATTGACCGCTGGTTCCTGGTACAGATTGAAGAGCTGGTGCGTCTGGAAGAGAAAGTGGCGGAAGTGGGCGTCACTGGc                                                                                   >  1:1735070/1‑93 (MQ=255)

      tCAACCTGACCAACATTGACCGCTGGTTCCTGGTACAGATTGAAGAGCTGGTGCGTCTGGAAGAGAAAGTGGCGGAAGTGGGCGTCACTGGcc                                                                                  >  1:1592628/1‑93 (MQ=255)

       cAACCTGACCAACATTGACCGCTGGTTCCTGGTACAGATTGAAGAGCTGGTGCGTCTGGAAGAGAAAGTGGCGGAAGTGGGCGTCACTGGCCt                                                                                 <  1:1576387/93‑1 (MQ=255)

       cAACCTGACCAACATTGACCGCTGGTTCCTGGTACAGATTGAAGAGCTGGTGCGTCTGGAAGAGAAAGTGGCGGAAGAGGGCGTCACTGGCCt                                                                                 >  2:2862879/1‑93 (MQ=255)

        aaCCTGACCAACATTGACCGCTGGTTCCTGGTACAGATTGAAGAGCTGGTGCGTCTGGAAGAGAAAGTGGCGGAAGTGGGCGTCACTGGCCTg                                                                                >  2:2275798/1‑93 (MQ=255)

        aaCCTGACCAACATTGACCGCTGGTTCCTGGTACAGATTGAAGAGCTGGTGCGTCTGGAAGAGAAAGTGGCGGAAGTGGGCGTCACTGGCCTg                                                                                >  2:3230356/1‑93 (MQ=255)

         aCCTGACCAACATTGACCGCTGGTTCCTGGTACAGATTGAAGAGCTGGTGCGTCTGGAAGAGAAAGTGGCGGAAGTGGGCGGCACCGGCCTGa                                                                               >  2:1115085/1‑93 (MQ=255)

          ccTGACCAACATTGACCGCTGGTTCCTGGTACAGATTGAAGAGCTGGTGCGTCTGGAAGAGAAAGTGGCGGAAGTGGGCGTCACTGGCCTGaa                                                                              <  2:3580491/93‑1 (MQ=255)

             gACCAACATTGACCGCTGGTTCCTGGTACAGATTGAAGAGCTGGTGCGTCTGGAAGAGAAAGTGGCGGAAGTGGGCGTCACTGGCCTGAACGc                                                                           <  1:106792/93‑1 (MQ=255)

             gACCAACATTGACCGCTGGTTCCTGGTACAGATTGAAGAGCTGGTGCGTCTGGAAGAGAAAGTGGCGGAAGTGGGCGTCACTGGCCTGAACGc                                                                           <  1:600670/93‑1 (MQ=255)

              aCCAACATTGACCGCTGGTTCCTGGTACAGATTGAAGAGCTGGTGCGTCTGGAAGAGAAAGTGGCGGAAGTGGGCGTCACTGGCCTGAACGCt                                                                          <  1:2912132/93‑1 (MQ=255)

               ccAACATTGACCGCTGGTTCCTGGTACAGATTGAAGAGCTGGTGCGTCTGGAAGAGAAAGTGGCGGAAGTGGGCGTCACTGGCCTGAACGCTg                                                                         <  1:807636/93‑1 (MQ=255)

                cAACATTGACCGCTGGTTCCTGGTACAGATTGAAGAGCTGGTGCGTCTGGAAGAGAAAGTGGCGGAAGTGGGCGTCACTGGCCTGAACGCTGa                                                                        >  1:180715/1‑93 (MQ=255)

                 aaCATTGACCGCTGGTTCCTGGTACAGATTGAAGAGCTGGTGCGTCTGGAAGAGAAAGTGGCGGTAGTGGGCGTCACTGGCCTGAACGCTGAc                                                                       >  1:4510702/1‑93 (MQ=255)

                 aaCATTGACCGCTGGTTCCTGGTACAGATTGAAGAGCTGGTGCGTCTGGAAGAGAAAGTGGCGGAAGTGGGCGTCACTGGCCTGAACGCTGAc                                                                       <  2:3491035/93‑1 (MQ=255)

                 aaCATTGACCGCTGGTTCCTGGTACAGATTGAAGAGCTGGTGCGTCTGGAAGAGAAAGTGGCGGAAGTGGGCGTCACTGGCCTGAACGCTGAc                                                                       >  2:550984/1‑93 (MQ=255)

                 aaCATTGACCGCTGGTTCCTGGTACAGATTGAAGAGCTGGTGCGTCTGGAAGAGAAAGTGGCGGAAGTGGGCGTCACTGGCCTGAACGCCGAc                                                                       >  2:2171054/1‑93 (MQ=255)

                  aCATTGACCGCTGGTTCCTGGTACAGATTGAAGAGCTGGTGCGTCTGGAAGAGAAAGTGGCGGAAGTGGGCGTCACTGGCCTGAACGCTGACt                                                                      >  2:3077571/1‑93 (MQ=255)

                  aCATTGACCGCTGGTTCCTGGTACAGATTGAAGAGCTGGTGCGTCTGGAAGAGAAAGTGGCGGAAGTGGGCGTCACTGGCCTGAACGCTGACt                                                                      <  1:2253953/93‑1 (MQ=255)

                    agtGACCGCTGGTTCCTGGTACAGATTGAAGAGCTGGTGCGTCTGGAAGAGAAAGTGGCGGAAGTGGGCGTCACTGGCCTGAACGCTGACTTc                                                                    >  2:3732921/3‑93 (MQ=255)

                    aTTGACCGCTGGTTCCTGGTACAGATTGAAGAGCTGGTGCGTCTGGAAGAGAAAGTGGCGGAAGTGGGCGTCACTGGCCTGAACGCTGACTTc                                                                    >  1:888073/1‑93 (MQ=255)

                      tGACCGCTGGTTCCTGGTACAGATTGAAGAGCTGGTGCGTCTGGAAGAGAAAGTGGCGGAAGTGGGCGTCACTGGCCTGAACGCTGACTTCCt                                                                  <  1:4544503/93‑1 (MQ=255)

                      tGACCGCTGGTTCCTGGTACAGATTGAAGAGCTGGTGCGTCTGGAAGAGAAAGTGGCGGAAGTGGGCGTCACTGGCCTGAACGCTGACTTCCt                                                                  <  2:2699111/93‑1 (MQ=255)

                       gACCGCTGGTTCCTGGTACAGATTGAAGAGCTGGTGCGTCTGGAAGAGAAAGTGGCGGAAGTGGGCGTCACTGGCCTGAACGCTGACTTCCTg                                                                 <  2:924739/93‑1 (MQ=255)

                       gACCGCTGGTTCCTGGTACAGATTGAAGAGCTGGTGCGTCTGGAAGAGAAAGTGGCGGAAGCGGGCGTCACTGGCCTGAACGCTGACTTCCTg                                                                 >  1:488868/1‑93 (MQ=255)

                        aCCGCTGGTTCCTGGTACAGATTGAAGAGCTGGTGCGTCTGGAAGAGAAAGTGGCGGAAGTGGGCGTCACTGGCCTGAACGCTGACTTCCTgc                                                                <  2:145492/93‑1 (MQ=255)

                          cGCTGGTTCCTGGTACAGATTGAAGAGCTGGTGCGTCTGGAAGAGAAAGTGGCGGAAGTGGGCGTCACTGGCCTGAACGCTGACTTCCTgcgc                                                              <  2:4130912/93‑1 (MQ=255)

                           gCTGGTTCCTGGTACAGATTGAAGAGCTGGTGCGTCTGGAAGAGAAAGTGGCGGAAGTGGGCGTCACTGGCCTGAACGCTGACTTCCTGCGcc                                                             <  1:1239609/93‑1 (MQ=255)

                           gCTGGTTCCTGGTACAGATTGAAGAGCTGGTGCGTCTGGAAGAGAAAGTGGCGGAAGTGGGCGTCACTGGCCTGAACGCTGACTTCCTGCGcc                                                             >  1:3379342/1‑93 (MQ=255)

                            cTGGTTCCTGGTACAGATTGAAGAGCTGGTGCGTCTGGAAGAGAAAGTGGCGGAAGTGGGCGTCACTGGCCTGAACGCTGACTTCCTGCGCCa                                                            >  1:2759970/1‑93 (MQ=255)

                             tGGTTCCTGGTACAGATTGAAGAGCTGGTGCGTCTGGAAGAGAAAGTGGCGGAAGTGGGCGTCACTGGCCTGAACGCTGACTTCCTGCGCCAg                                                           >  1:4331227/1‑93 (MQ=255)

                              ggTTCCTGGTACAGATTGAAGAGCTGGTGCGTCTGGAAGAGAAAGTGGCGGAAGTGGGCGTCACTGGCCTGAACGCTGACTTCCTGCGCCAGc                                                          <  2:3373375/93‑1 (MQ=255)

                              ggTTCCTGGTACAGATTGAAGAGCTGGTGCGTCTGGAAGAGAAAGTGGCGGAAGTGGGCGTCACTGGCCTGAACGCTGACTTCCTGCGCCAGc                                                          <  1:2328749/93‑1 (MQ=255)

                               gTTCCTGGTACAGATTGAAGAGCTGGTGCGTCTGGAAGAGAAAGTGGCGGAAGTGGGCGTCACTGGCCTGAACGCTGACTTCCTGCGCCAGcg                                                         >  1:1078358/1‑92 (MQ=255)

                               gTTCCTGGTACAGATTGAAGAGCTGGTGCGTCTGGAAGAGAAAGTGGCGGAAGTGGGCGTCACTGGCCTGAACGCTGACTTCCTGCGCCAGCt                                                         >  1:372581/1‑93 (MQ=255)

                                   cTGGTACAGATTGAAGAGCTGGTGCGTCTGGAAGAGAAAGTGGCGGAAGTGGGCGTCACTGGCCTGAACGCTGACTTCCTGCGCCAGCTGaaa                                                     <  2:2054688/93‑1 (MQ=255)

                                   cTGGTACAGATTGAAGAGCTGGTGCGTCTGGAAGAGAAAGTGGCGGAAGTGGGCGTCACTGGCCTGAACGCTGACTTCCTGCGCCAGCTGaaa                                                     <  2:3130182/93‑1 (MQ=255)

                                   cTGGTACAGATTGAAGAGCTGGTGCGTCTGGAAGAGAAAGTGGCGGAAGTGGGCGTCACTGGCCTGAACGCTGACTTCCTGCGCCAGCTGaaa                                                     >  1:512477/1‑93 (MQ=255)

                                    tGGTACAGATTGAAGAGCTGGTGCGTCTGGAAGAGAAAGTGGCGGAAGTGGGCGTTACTGGCCTGAACGCTGACTTTCTGCGCCAGCTGAAAc                                                    >  2:274011/1‑93 (MQ=255)

                                    tGGTACAGATTGAAGAGCTGGTGCGTCTGGAAGAGAAAGTGGCGGAAGTGGGCGTCACTGGCCTGAACGCTGACTTCCTGCGCCAGCTGAAAc                                                    <  1:2552656/93‑1 (MQ=255)

                                    tGGTACAGATTGAAGAGCTGGTGCGTCTGGAAGAGAAAGTGGCGGAAGTGGGCGTCACTGGCCTGAACGCTGACTTCCTGCGCCAGCTGAAAc                                                    >  2:829344/1‑93 (MQ=255)

                                    tGGTACAGATTGAAGAGCTGGTGCGTCTGGAAGAGAAAGTGGCGGAAGTGGGCGTCACTGGCCTCAACGCTGACTTCCTGCGCCAGCTGAAAc                                                    <  1:4068165/93‑1 (MQ=255)

                                       tACAGATTGAAGAGCTGGTGCGTCTGGAAGAGAAAGTGGCGGAAGTGGGCGTCACTGGCCTGAACGCTGACTTCCTGCGCCAGCTGAAACGCa                                                 <  1:3151129/93‑1 (MQ=255)

                                        aCAGATTGAAGAGCTGGTGCGTCTGGAAGAGAAAGTGGCGGAAGTGGGCGTCACTGGCCTGAACGCTGACTTCCTGCGCCAGCTGAAACGCaa                                                >  2:4435143/1‑93 (MQ=255)

                                         cAGATTGAAGAGCTGGTGCGTCTGGAAGAGAAAGTGGCGGAAGTGGGCGTCACTGGCCTGAACGCTGACTTCCTGCGCCAGCTGAAACGCaaa                                               <  1:2284780/93‑1 (MQ=255)

                                         cAGATTGAAGAGCTGGTGCGTCTGGAAGAGAAAGTGGCGGAAGTGGGCGTCACTGGCCTGAACGCTGACTTCCTGCGCCAGCTGAAACGCaaa                                               <  1:905170/93‑1 (MQ=255)

                                         cAGATTGAAGAGCTGGTGCGTCTGGAAGAGAAAGTGGCGGAAGTGGGCGTCACTGGCCTGAACGCTGACTTCCTGCGCCAGCTGAAACGCaaa                                               >  1:1128314/1‑93 (MQ=255)

                                           gATTGAAGAGCTGGTGCGTCTGGAAGAGAAAGTGGCGGAAGTGGGCGTCACTGGCCTGAACGCTGACTTCCTGCGCCAGCTGAAACGCAAAgg                                             <  1:941434/93‑1 (MQ=255)

                                           gATTGAAGAGCTGGTGCGTCTGGAAGAGAAAGTGGCGGAAGTGGGCGTCACTGGCCTGAACGCTGACTTCCTGCGCCAGCTGAAACGCAAAgg                                             >  1:3415243/1‑93 (MQ=255)

                                            aTTGAAGAGCTGGTGCGTCTGGAAGAGAAAGTGGCGGAAGTGGGCGTCACTGGCCTGAACGCTGACTTCCTGCGCCAGCTGAAACGCAAAGGc                                            <  2:3636350/93‑1 (MQ=255)

                                            aTTGAAGAGCTGGTGCGTCTGGAAGAGAAAGTGGCGGAAGTGGGCGTCACTGGCCTGAACGCTGACTTCCTGCGCCAGCTGAAACGCAAAGGc                                            <  2:1728072/93‑1 (MQ=255)

                                               gAAGAGCTGGTGCGTCTGGAAGAGAAAGTGGCGGAAGTGGGCGTCACTGGCCTGAACGCTGACTTCCTGCGCCAGCTGAAACGCAAAGGCttt                                         >  2:1172930/1‑93 (MQ=255)

                                                aaGAGCTGGTGCGTCTGGAAGAGAAAGTGGCGGAAGTGGGCGTCACTGGCCTGAACGCTGACTTCCTGCGCCAGCTGAAACGCAAAGGCTTTg                                        >  1:3645273/1‑93 (MQ=255)

                                                aaGAGCTGGTGCGTCTGGAAGAGAAAGTGGCGGAAGTGGGCGTCACTGGCCTGAACGCTGACTTCCTGCGCCAGCTGAAACGCAAAGGCTTTg                                        >  1:3541235/1‑93 (MQ=255)

                                                  gagCTGGTGCGTCTGGAAGAGAAAGTGGCGGAAGTGGGCGTCACTGGCCTGAACGCTGACTTCCTGCGCCAGCTGAAACGCAAAGGCTTTGcc                                      <  2:3523063/93‑1 (MQ=255)

                                                    gCTGGTGCGTCTGGAAGAGAAAGTGGCGGAAGTGGGCGTCACTGGCCTGAACGCTGACTTCCTGCGCCAGCTGAAACGCAAAGGAGTTGGCGa                                    >  2:3980184/1‑93 (MQ=255)

                                                     cTGGTGCGTCTGGAAGAGAAAGTGGCGGAAGTGGGCGTCACTGGCCTGAACGCTGACTTCCTGCGCCAGCTGAAACGCAAAGGCTTTGCCGAt                                   <  2:2896907/93‑1 (MQ=255)

                                                      tGGTGCGTCTGGAAGAGAAAGTGGCGGAAGTGGGCGTCACTGGCCTGAACGCTGACTTCCTGCGCCAGCTGAAACGCAAAGGCTTTGCCGATg                                  >  1:2415585/1‑93 (MQ=255)

                                                       ggTGCGTCTGGAAGAGAAAGTGGCGGAAGTGGGCGTCACTGGCCTGAACGCTGACTTCCTGCGCCAGCTGAAACGCAAAGGCTTTGCCGATgc                                 <  1:4590001/93‑1 (MQ=255)

                                                       ggTGCGTCTGGAAGAGAAAGTGGCGGAAGTGGGCGTCACTGGCCTGAACGCTGACTTCCTGCGCCAGCTGAAACGCAAAGGCTTTGCCGATgc                                 <  1:1209732/93‑1 (MQ=255)

                                                       ggTGCGTCTGGAAGAGAAAGTGGCGGAAGTGGGCGTCACTGGCCTGAACGCTGACTTCCTGCGCCAGCTGAAACGCAAAGGCTTTGCCGATgc                                 >  2:3669509/1‑93 (MQ=255)

                                                       ggTGCGTCTGGAAGAGAAAGTGGCGGAAGTGGGCGTCACTGGCCTGAACGCTGACTTCCTGCGCCAGCTGAAACGCAAAGGCTTTGCCGATgc                                 <  1:3149219/93‑1 (MQ=255)

                                                         tGCGTCTGGAAGAGAAAGTGGCGGAAGTGGGCGTCACTGGCCTGAACGCTGACTTCCTGCGCCAGCTGAAACGCAAAGGCTTTGCCGATgcgc                               <  2:2034317/93‑1 (MQ=255)

                                                          gCGTCTGGAAGAGAAAGTGGCGGAAGTGGGCGTCACTGGCCTGAACGCTGACTTCCTGCGCCAGCTGAAACGCAAAGGCTTTGCCGATgcgcg                              <  1:1642262/93‑1 (MQ=255)

                                                            gTCTGGAAGAGAAAGTGGCGGAAGTGGGCGTCACTGGCCTGAACGCTGACTTCCTGCGCCAGCTGAAACGCAAAGGCTTTGCCGATGCGCGCt                            >  1:1966366/1‑93 (MQ=255)

                                                            gTCTGGAAGAGAAAGTGGCGGAAGTGGGCGTCACTGGCCTGAACGCTGACTTCCTGCGCCAGCTGAAACGCAAAGGCTTTGCCGATGCGCGCt                            >  2:497341/1‑93 (MQ=255)

                                                            gTCTGGAAGAGAAAGTGGCGGAAGTGGGCGTCACTGGCCTGAACGCTGACTTCCTGCGCCAGCTGAAACGCAAAGGCTTTGCCCATGCGCGCt                            >  1:3676093/1‑93 (MQ=255)

                                                            gTCTGGAAGAGAAAGGGGCGGAAGTGGGCGTCACTGGCCTGAACGCTGACTTCCTGCGCCAGCTGAAACGCAAAGGCTTTGCCGATGCGAGCt                            >  1:1392078/1‑93 (MQ=255)

                                                             tCTGGAAGAGAAAGTGGCGGAAGTGGGCGTCACTGGCCTGAACGCTGACTTCCTGCGCCAGCTGAAACGCAAAGGCTTTGCCGATGCGCGCtt                           <  2:1406675/93‑1 (MQ=255)

                                                             tCTGGAAGAGAAAGTGGCGGAAGTGGGCGTCACTGGCCTGAACGCTGACTTCCTGCGCCAGCTGAAACCCAAAAGCTTTGCCGATGCGCGCtt                           >  1:1246031/1‑93 (MQ=255)

                                                              cTGGAAGAGAAAGTGGCGGAAGTGGGCGTCACTGGCCTGAACGCTGACTTCCTGCGCCAGCTGAAACGCAAAGGCTTTGCCGATGCGCGCTTg                          >  2:2901921/1‑93 (MQ=255)

                                                                 gAAGAGAAAGTGGCGGAAGTGGGCGTCACTGGCCTGAACGCTGACTTCCTGCGCCAGCTGAAACGCAAAGGCTTTGCCGATGCGCGCTTGGCa                       >  2:4266138/1‑93 (MQ=255)

                                                                  aaGAGAAAGTGGCGGAAGTGGGCGTCACTGGCCTGAACGCTGACTTCCTGCGCCAGCTGAAACGCAAAGGCTTTGCCGATGCGCGCTTGGCaa                      <  2:3309747/93‑1 (MQ=255)

                                                                  aaGAGAAAGTGGCGGAAGTGGGCGTCACTGGCCTGAACGCTGACTTCCTGCGCCAGCTGAAACGCAAAGGCTTTGCCGATGCGCGCTTGGCaa                      >  1:2442913/1‑93 (MQ=255)

                                                                   agagAAAGTGGCGGAAGTGGGCGTCACTGGCCTGAACGCTGACTTCCTGCGCCAGCTGAAACGCAAAGGCTTTGCCGATGCGCGCTTGGCaaa                     >  1:2700275/1‑93 (MQ=255)

                                                                     agaAAGTGGCGGAAGTGGGCGTCACTGGCCTGAACGCTGACTTCCTGCGCCAGCTGAAACGCAAAGGCTTTGCCGATGCGCGCTTGGCAAAAc                   >  1:407999/1‑93 (MQ=255)

                                                                     agaAAGTGGCGGAAGTGGGCGTCACTGGCCTGAACGCTGACTTCCTGCGCCAGCTGAAACGCAAAGGCTTTGCCGATGCGCGCTTGGCAAAAc                   <  2:1555844/93‑1 (MQ=255)

                                                                       aaaGTGGCGGAAGTGGGCGTCACTGGCCTGAACGCTGACTTCCTGCGCCAGCTGAAACGCAAAGGCTTTGCCGATGCGCGCTTGGCAAAACTg                 <  1:3828570/93‑1 (MQ=255)

                                                                           tGGCGGAAGTGGGCGTCACTGGCCTGAACGCTGACTTCCTGCGCCAGCTGAAACGCAAAGGCTTTGCCGATGCGCGCTTGGCAAAACTggcgg             <  2:153249/93‑1 (MQ=255)

                                                                           tGGCGGAAGTGGGCGTCACTGGCCTGAACGCTGACTTCCTGCGCCAGCTGAAACGCAAAGGCTTTGCCGATGCGCGCTTGGCAAAACTggcgg             <  1:1985102/93‑1 (MQ=255)

                                                                               ggAAGTGGGCGTCACTGGCCTGAACGCTGACTTCCTGCGGCAGCTGAAACGCAAAGGCTTTGCCGATGCGCGCTTGGCAAAACTggcgggcgg         >  2:1653057/1‑92 (MQ=255)

                                                                               ggAAGTGGGCGTCACTGGCCTGAACGCTGACTTCCTGCGCCAGCTGAAACGCAAAGGCTTTGCCGATGCGCGCTTGGCAAAACTGGCGGGCGt         <  2:1168359/93‑1 (MQ=255)

                                                                                gAAGTGGGCGTCACTGGCCTGAACGCTGACTTCCTGCGCCAGCTGAAACGCAAAGGCTTTGCCGATGCGCGCTTGGCAAAACTggcgggcgga        >  1:99334/1‑91 (MQ=255)

                                                                                gAAGTGGGCGTCACTGGCCTGAACGCTGACTTCCTGCGCCAGCTGAAACGCAAAGGCTTTGCCGATGCGCGCTTGGCAAAACTGGCGGGGGtc        >  1:1149372/1‑92 (MQ=255)

                                                                                 aaGTGGGCGTCACTGGCCTGAACGCTGACTTCCTGCGCCAGCTGAAACGCAAAGGCTTTGCCGATGCGCGCTTGGCAAAACTGGCGGGCGTAc       <  1:1861820/93‑1 (MQ=255)

                                                                                  aGTGGGCGTCACTGGCCTGAACGCTGACTTCCTGCGCCAGCTGAAACGCAAAGGCTTTGCCGATGCGCGCTTGGCAAAACTGGCGGGCGTAcg      <  2:3173733/93‑1 (MQ=255)

                                                                                   gTGGGCGTCACTGGCCTGAACGCTGACTTCCTGCGCCAGCTGAAACGCAAAGGCTTTGCCGATGCGCGCTTGGCAAAACTGGCGGGCGTAcgc     <  2:4016988/93‑1 (MQ=255)

                                                                                   gTGGGCGTCACTGGCCTGAACGCTGACTTCCTGCGCCAGCTGAAACGCAAAGGCTTTGCCGATGCGCGCTTGGCAAAACTGGCGGGCGTAcgc     <  2:2080595/93‑1 (MQ=255)

                                                                                   gTGGGCGTCACTGGCCTGAACGCTGACTTCCTGCGCCAGCTGAAACGCAAAGGCTTTGCCGATGCGCGCTTGGCAAAACTGGCGGGCGTAcgc     >  1:208906/1‑93 (MQ=255)

                                                                                    tGGGCGTCACTGGCCTGAACGCTGACTTCCTGCGCCAGCTGAAACGCAAAGGCTTTGCCGATGCGCGCTTGGCAAAACTGGCGGGCGTAcgcg    >  2:4188989/1‑93 (MQ=255)

                                                                                      ggCGTCACTGGCCTGAACGCTGACTTCCTGCGCCAGCTGAAACGCAAAGGCTTTGCCGATGCGCGCTTGGCAAAACTGGCGGGCGTACGCGaa  <  2:1894215/93‑1 (MQ=255)

                                                                                      ggCGTCACTGGCCTGAACGCTGACTTCCTGCGCCAGCTGAAACGCAAAGGCTTTGCCGATGCGCGCTTGGCAAAACTGGCGGGCGTACGCGaa  <  1:3604943/93‑1 (MQ=255)

GCGTCTTCAACCTGACCAACATTGACCGCTGGTTCCTGGTACAGATTGAAGAGCTGGTGCGTCTGGAAGAGAAAGTGGCGGAAGTGGGCATCACTGGCCTGAACGCTGACTTCCTGCGCCAGCTGAAACGCAAAGGCTTTGCCGATGCGCGCTTGGCAAAACTGGCGGGCGTACGCGAA  >  NC_000913/32168‑32346

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 18 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`